Lag0002956 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002956
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Auxin-responsive protein
Location	chr4: 46984915 .. 46985979 (-)
RNA-Seq Expression	Lag0002956
Synteny	Lag0002956

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAAGAAGCCCTTGGACTCGAGATCACCGAGCTTCGTCTCGGTCTCCCCGGCAACAGCGACAAAAATGAGAAGAAACGAGTCTATGCTGACATCGTTGGTGGAGACGAGCCGACCAAGAACGATGTCGGTAGGAAGAATAATGCGAGTAAGGGACAAGTCGTGGGGTGGCCACCGGTGTGTTCGTATCGGAAAAAGAATAGTTTTGGAGAGAAAGAGTCGTCGTCGTCGTTGCATGAGAATGAGAATTCGAAAATGTATGTGAAGATTAGCATGGATGGAGCTCCATTTCTGCGTAAACTTGACTTGGGTTCTCATAAAGGATACTCGGATCTTGCCCTTGCTCTTGAAAAGCTCTTTGCTTGCTTTGGAACTGGTAAATTTAGAGCTCTCTATCTCTCCTTTAATTTGTTATTAAACTTTTTTTAGGGTTTATGTAGTAACGTGCTTAATAAGTTAATATTGCATGGTAAATTATATGAGAGATTTATAATCATTTAATTAGTAGTAATATTTCGAGGAATTTGATGTGTAAATAAAAGAGTAACATATTTGGAAGACTAAATTCTTACTGATTTATTGAGTTTTGTAAAAATAATTGAGTTAGTCAAAAATAGGATGGACCCAAGAAAATTTTATGAGAAATGAAGCAAAATTGTTATATACATAATTTTCCAATTTTTGAAAAATTTAAGGGACAAATCAGAAGAAGTATAAGATTTTGATTTTTATATATATATTTTTTAACTTAACAAAGTTAAAATAGAGCAATAGATCATCTCATTGATGATAGGGGAAATTGTAAATCTAAAAATGAGATAAAATTTAGAAAAATAAGTGGAGTAAAAAAAAATACATAATATTAGATGCATATTTGTTTATTTATTTTTAAATAATAAAAAAAAAAGTATATTTATGTAGAAAAATATAATGAGAAAGTAGTGGCATATTTGTAAAATGACAGGGAAGACATTAAAGCATGGAGAAAGTTCTGATTACGTTCCCATTTATGAGGACAAGGATGGGGACTGGATGCTTGTTGGAGACGTCCCTTGGGAGTAA

mRNA sequence

ATGGCAAAAGAAGCCCTTGGACTCGAGATCACCGAGCTTCGTCTCGGTCTCCCCGGCAACAGCGACAAAAATGAGAAGAAACGAGTCTATGCTGACATCGTTGGTGGAGACGAGCCGACCAAGAACGATGTCGGTAGGAAGAATAATGCGAGTAAGGGACAAGTCGTGGGGTGGCCACCGGTGTGTTCGTATCGGAAAAAGAATAGTTTTGGAGAGAAAGAGTCGTCGTCGTCGTTGCATGAGAATGAGAATTCGAAAATGTATGTGAAGATTAGCATGGATGGAGCTCCATTTCTGCGTAAACTTGACTTGGGTTCTCATAAAGGATACTCGGATCTTGCCCTTGCTCTTGAAAAGCTCTTTGCTTGCTTTGGAACTGGGAAGACATTAAAGCATGGAGAAAGTTCTGATTACGTTCCCATTTATGAGGACAAGGATGGGGACTGGATGCTTGTTGGAGACGTCCCTTGGGAGTAA

Coding sequence (CDS)

ATGGCAAAAGAAGCCCTTGGACTCGAGATCACCGAGCTTCGTCTCGGTCTCCCCGGCAACAGCGACAAAAATGAGAAGAAACGAGTCTATGCTGACATCGTTGGTGGAGACGAGCCGACCAAGAACGATGTCGGTAGGAAGAATAATGCGAGTAAGGGACAAGTCGTGGGGTGGCCACCGGTGTGTTCGTATCGGAAAAAGAATAGTTTTGGAGAGAAAGAGTCGTCGTCGTCGTTGCATGAGAATGAGAATTCGAAAATGTATGTGAAGATTAGCATGGATGGAGCTCCATTTCTGCGTAAACTTGACTTGGGTTCTCATAAAGGATACTCGGATCTTGCCCTTGCTCTTGAAAAGCTCTTTGCTTGCTTTGGAACTGGGAAGACATTAAAGCATGGAGAAAGTTCTGATTACGTTCCCATTTATGAGGACAAGGATGGGGACTGGATGCTTGTTGGAGACGTCCCTTGGGAGTAA

Protein sequence

MAKEALGLEITELRLGLPGNSDKNEKKRVYADIVGGDEPTKNDVGRKNNASKGQVVGWPPVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGYSDLALALEKLFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE

Homology

[Close]

BLAST of Lag0002956 vs. NCBI nr
Match: XP_038899110.1 (auxin-induced protein AUX22-like [Benincasa hispida])

HSP 1 Score: 288.9 bits (738), Expect = 2.7e-74
Identity = 141/159 (88.68%), Postives = 150/159 (94.34%), Query Frame = 0


Query: 1   MAKEALGLEITELRLGLPGNSDKNEKKRVYADIVGGDEPTKNDVGRKNNAS-KGQVVGWP 60
           MAKE+LGLEITELRLGLPGN DKNEKKRVYADIV  DEP ++DV +KN+ + KGQVVGWP
Sbjct: 1   MAKESLGLEITELRLGLPGNDDKNEKKRVYADIVNEDEPARDDVEQKNSMTIKGQVVGWP 60

Query: 61  PVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGYSDLALALEK 120
           PVCSYRKKNSFGEKE  S+LHE+ENSKMYVKISMDGAPFLRKLDLGSHKGYSDLALAL+K
Sbjct: 61  PVCSYRKKNSFGEKE--STLHESENSKMYVKISMDGAPFLRKLDLGSHKGYSDLALALQK 120

Query: 121 LFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 159
           LFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE
Sbjct: 121 LFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 157

[Close]

BLAST of Lag0002956 vs. NCBI nr
Match: KAG7014105.1 (Auxin-induced protein AUX22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 287.0 bits (733), Expect = 1.0e-73
Identity = 141/159 (88.68%), Postives = 148/159 (93.08%), Query Frame = 0


Query: 1   MAKEALGLEITELRLGLPGNSDKNEKKRVYADIVGGDEPTKNDVGRKNN-ASKGQVVGWP 60
           MA E+LGLEITELRLGLPGN+DKNEKKR YADIV  DEPT++DVGRKNN  SKGQVVGWP
Sbjct: 56  MANESLGLEITELRLGLPGNNDKNEKKRAYADIVDEDEPTRDDVGRKNNTTSKGQVVGWP 115

Query: 61  PVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGYSDLALALEK 120
           PVCSYRKKNSFGEKE   SL +NENSKMYVKISMDGAPFLRKLDLGSH+GYSDLA+ALEK
Sbjct: 116 PVCSYRKKNSFGEKE---SLLKNENSKMYVKISMDGAPFLRKLDLGSHEGYSDLAVALEK 175

Query: 121 LFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 159
           LFACFGTGKT KHGESSDYVPIYEDKDGDWMLVGDVPWE
Sbjct: 176 LFACFGTGKTSKHGESSDYVPIYEDKDGDWMLVGDVPWE 211

[Close]

BLAST of Lag0002956 vs. NCBI nr
Match: KAG6575564.1 (Auxin-induced protein AUX22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 285.0 bits (728), Expect = 3.9e-73
Identity = 140/159 (88.05%), Postives = 148/159 (93.08%), Query Frame = 0


Query: 1   MAKEALGLEITELRLGLPGNSDKNEKKRVYADIVGGDEPTKNDVGRKNN-ASKGQVVGWP 60
           MA E+LGLEITELRLGLPGN+DKNEKKR YADIV  DEPT++DVGRKNN  SKGQVVGWP
Sbjct: 1   MANESLGLEITELRLGLPGNNDKNEKKRAYADIVDEDEPTRDDVGRKNNTTSKGQVVGWP 60

Query: 61  PVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGYSDLALALEK 120
           PVCSYRKKNSFGEKE   SL +NENSKMYVKISMDGAPFLRKLDLGSH+GYSDLA+ALEK
Sbjct: 61  PVCSYRKKNSFGEKE---SLLKNENSKMYVKISMDGAPFLRKLDLGSHEGYSDLAVALEK 120

Query: 121 LFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 159
           LFACFGTGKT KHGESS+YVPIYEDKDGDWMLVGDVPWE
Sbjct: 121 LFACFGTGKTSKHGESSNYVPIYEDKDGDWMLVGDVPWE 156

[Close]

BLAST of Lag0002956 vs. NCBI nr
Match: XP_022954061.1 (auxin-induced protein AUX22 [Cucurbita moschata])

HSP 1 Score: 282.7 bits (722), Expect = 1.9e-72
Identity = 139/159 (87.42%), Postives = 146/159 (91.82%), Query Frame = 0


Query: 1   MAKEALGLEITELRLGLPGNSDKNEKKRVYADIVGGDEPTKNDVGRKNN-ASKGQVVGWP 60
           MA E+LGLEITELRLGLPGN+DKNEKKR YADIV  DEPT++DV RKNN  SKGQVVGWP
Sbjct: 1   MANESLGLEITELRLGLPGNNDKNEKKRAYADIVDEDEPTRDDVDRKNNTTSKGQVVGWP 60

Query: 61  PVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGYSDLALALEK 120
           PVCSYRKKNSFGEKE   SL +NENSKMYVKISMDGAPFLRKLDLGSH+GYSDLA+ALEK
Sbjct: 61  PVCSYRKKNSFGEKE---SLLKNENSKMYVKISMDGAPFLRKLDLGSHEGYSDLAVALEK 120

Query: 121 LFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 159
           LF CFGTGKT KHGESSDYVPIYEDKDGDWMLVGDVPWE
Sbjct: 121 LFGCFGTGKTSKHGESSDYVPIYEDKDGDWMLVGDVPWE 156

[Close]

BLAST of Lag0002956 vs. NCBI nr
Match: XP_004135828.1 (auxin-induced protein IAA6 [Cucumis sativus])

HSP 1 Score: 282.3 bits (721), Expect = 2.5e-72
Identity = 144/167 (86.23%), Postives = 150/167 (89.82%), Query Frame = 0


Query: 1   MAKEALGLEITELR--LGLPGNSDKNEKKRVYADIVGGD-EPTK----NDVGRKNN--AS 60
           MAKE+LG EITELR  LGLPGNS+KNEKKR + DIV GD EPT+    N VGRKN+   S
Sbjct: 1   MAKESLGFEITELRLGLGLPGNSNKNEKKRAFDDIVNGDHEPTRDNDDNRVGRKNSNTTS 60

Query: 61  KGQVVGWPPVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGYS 120
           KGQVVGWPPVCSYRK+NSFGEKESSS LHENENSKMYVKISMDGAPFLRKLDLGSHKGYS
Sbjct: 61  KGQVVGWPPVCSYRKRNSFGEKESSSLLHENENSKMYVKISMDGAPFLRKLDLGSHKGYS 120

Query: 121 DLALALEKLFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 159
           DLALALEKLFACFGTGKTLKHGES DYVPIYEDKDGDWMLVGDVPWE
Sbjct: 121 DLALALEKLFACFGTGKTLKHGESCDYVPIYEDKDGDWMLVGDVPWE 167

[Close]

BLAST of Lag0002956 vs. ExPASy Swiss-Prot
Match: P13088 (Auxin-induced protein AUX22 OS=Glycine max OX=3847 GN=AUX22 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 5.6e-51
Identity = 104/168 (61.90%), Postives = 121/168 (72.02%), Query Frame = 0


Query: 1   MAKEALGLEITELRLGLPGN-------SDKNEKKRVYADI---VGGDEPTKNDVGRKNNA 60
           MAKE LGLEITELRLGLP         + KNEKKR +++I   VG +  +     RK   
Sbjct: 1   MAKEGLGLEITELRLGLPDAEHQVSVVNKKNEKKRAFSEIDDGVGDENSSSGGGDRKMET 60

Query: 61  SKGQVVGWPPVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGY 120
           +K QVVGWPPVCSYRKKNS  E            SKMYVK+SMDGAPFLRK+DLG HKGY
Sbjct: 61  NKSQVVGWPPVCSYRKKNSMNEGA----------SKMYVKVSMDGAPFLRKIDLGLHKGY 120

Query: 121 SDLALALEKLFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 159
           SDLALAL+KLF C+G  + LK+ ++S++VPIYEDKDGDWMLVGDVPWE
Sbjct: 121 SDLALALDKLFGCYGMVEALKNADNSEHVPIYEDKDGDWMLVGDVPWE 158

[Close]

BLAST of Lag0002956 vs. ExPASy Swiss-Prot
Match: P32293 (Auxin-induced protein 22A OS=Vigna radiata var. radiata OX=3916 GN=AUX22A PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 3.1e-49
Identity = 101/168 (60.12%), Postives = 119/168 (70.83%), Query Frame = 0


Query: 1   MAKEALGLEITELRLGLP--------GNSDKNEKKRVYADI--VGGDEPTKNDVGRKNNA 60
           MAKE LGLEITELRLGLP          + + + KRV+++I  VG +  +    G +   
Sbjct: 1   MAKEGLGLEITELRLGLPDAEHVAVANKNGEKKNKRVFSEIDDVGDENSSSGGGGDRKME 60

Query: 61  SKGQVVGWPPVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGY 120
           +K QVVGWPPVCSYRKKNS            NE SKMYVK+SMDGAPFLRK+DLG HKGY
Sbjct: 61  NKNQVVGWPPVCSYRKKNSV-----------NEASKMYVKVSMDGAPFLRKMDLGMHKGY 120

Query: 121 SDLALALEKLFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 159
           SDLA ALEKLF C+G  + LK+ E+ ++VPIYEDKDGDWMLVGDVPWE
Sbjct: 121 SDLAFALEKLFGCYGMVEALKNVENGEHVPIYEDKDGDWMLVGDVPWE 157

[Close]

BLAST of Lag0002956 vs. ExPASy Swiss-Prot
Match: P49680 (Auxin-induced protein IAA6 OS=Pisum sativum OX=3888 GN=IAA6 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 6.9e-49
Identity = 100/159 (62.89%), Postives = 115/159 (72.33%), Query Frame = 0


Query: 1   MAKEALGLEITELRLGLP-GNSDKNEKKRVYADIVGGDEPTKNDVGRKNNASKGQVVGWP 60
           MA+E LGLEITELRLGL  G   KNEKKR++++I GG E       RK+   K QVVGWP
Sbjct: 1   MAREGLGLEITELRLGLSCGEPKKNEKKRMFSEIDGGVEENGGSGDRKSVDKKNQVVGWP 60

Query: 61  PVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGYSDLALALEK 120
           PVCSYRKKN             NE SKMY+K+SMDGAP+LRK+DL  HKGY +LALALEK
Sbjct: 61  PVCSYRKKNM------------NEGSKMYMKVSMDGAPYLRKIDLCLHKGYLELALALEK 120

Query: 121 LFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 159
           LF C G  + LK  E+ ++VPIYEDKDGDWMLVGDVPWE
Sbjct: 121 LFDCCGIEEALKDAENCEHVPIYEDKDGDWMLVGDVPWE 147

[Close]

BLAST of Lag0002956 vs. ExPASy Swiss-Prot
Match: O24541 (Auxin-induced protein 22C OS=Vigna radiata var. radiata OX=3916 GN=AUX22C PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 4.5e-48
Identity = 100/166 (60.24%), Postives = 121/166 (72.89%), Query Frame = 0


Query: 1   MAKEALGLEITELRLGLPG-----NSDKNE---KKRVYADIVGGDEPTKNDVGRKNNASK 60
           M KE LGLEITELRLGLPG     N+DK++   KKRV++DI G +  ++ D G+K   +K
Sbjct: 1   MEKEDLGLEITELRLGLPGAGGENNTDKDKNKNKKRVFSDIEGENSSSEED-GKKE--TK 60

Query: 61  GQVVGWPPVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLRKLDLGSHKGYSD 120
            QVVGWPPVCSYRKKN+  E             K+YVK+SMDGAPFLRK+DL  HKGYSD
Sbjct: 61  NQVVGWPPVCSYRKKNTVNE------------PKLYVKVSMDGAPFLRKIDLAMHKGYSD 120

Query: 121 LALALEKLFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPWE 159
           LA AL+K F C+G  + LK  E++++VPIYEDKDGDWMLVGDVPWE
Sbjct: 121 LAFALDKFFGCYGICEALKDAENAEHVPIYEDKDGDWMLVGDVPWE 151

[Close]

BLAST of Lag0002956 vs. ExPASy Swiss-Prot
Match: O24409 (Auxin-responsive protein IAA19 OS=Arabidopsis thaliana OX=3702 GN=IAA19 PE=1 SV=2)

HSP 1 Score: 168.3 bits (425), Expect = 6.9e-41
Identity = 95/177 (53.67%), Postives = 114/177 (64.41%), Query Frame = 0


Query: 1   MAKEALGLEITELRLGLPGN--SDKNEKKRVYADI------------------VGGDEPT 60
           M KE LGLEITELRLGLPG   ++K  KKR + ++                   GGD   
Sbjct: 1   MEKEGLGLEITELRLGLPGRDVAEKMMKKRAFTEMNMTSSGSNSDQCESGVVSSGGDAEK 60

Query: 61  KNDVGRKNNASKGQVVGWPPVCSYRKKNSFGEKESSSSLHENENSKMYVKISMDGAPFLR 120
            ND    + A+K QVVGWPPVCSYRKKNS   KE+S++    +    YVK+SMDG P+LR
Sbjct: 61  VND----SPAAKSQVVGWPPVCSYRKKNSC--KEASTT----KVGLGYVKVSMDGVPYLR 120

Query: 121 KLDLGSHKGYSDLALALEKLFACFGTGKTLKHGESSDYVPIYEDKDGDWMLVGDVPW 158
           K+DLGS +GY DLA AL+KLF   G G  LK G++ +YV IYEDKDGDWML GDVPW
Sbjct: 121 KMDLGSSQGYDDLAFALDKLFGFRGIGVALKDGDNCEYVTIYEDKDGDWMLAGDVPW 167

The following BLAST results are available for this feature:

BLAST of Lag0002956 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899110.1	2.7e-74	88.68	auxin-induced protein AUX22-like [Benincasa hispida]	[more]
KAG7014105.1	1.0e-73	88.68	Auxin-induced protein AUX22, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
KAG6575564.1	3.9e-73	88.05	Auxin-induced protein AUX22, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022954061.1	1.9e-72	87.42	auxin-induced protein AUX22 [Cucurbita moschata]	[more]
XP_004135828.1	2.5e-72	86.23	auxin-induced protein IAA6 [Cucumis sativus]	[more]

BLAST of Lag0002956 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P13088	5.6e-51	61.90	Auxin-induced protein AUX22 OS=Glycine max OX=3847 GN=AUX22 PE=2 SV=1	[more]
P32293	3.1e-49	60.12	Auxin-induced protein 22A OS=Vigna radiata var. radiata OX=3916 GN=AUX22A PE=2 S...	[more]
P49680	6.9e-49	62.89	Auxin-induced protein IAA6 OS=Pisum sativum OX=3888 GN=IAA6 PE=2 SV=1	[more]
O24541	4.5e-48	60.24	Auxin-induced protein 22C OS=Vigna radiata var. radiata OX=3916 GN=AUX22C PE=2 S...	[more]
O24409	6.9e-41	53.67	Auxin-responsive protein IAA19 OS=Arabidopsis thaliana OX=3702 GN=IAA19 PE=1 SV=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR033389	AUX/IAA domain	PFAM	PF02309	AUX_IAA	coord: 46..158 e-value: 4.1E-38 score: 131.6
None	No IPR available	GENE3D	3.10.20.90		coord: 80..158 e-value: 6.1E-29 score: 102.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..53
None	No IPR available	PANTHER	PTHR31734:SF87	AUXIN-RESPONSIVE PROTEIN IAA6	coord: 4..158
None	No IPR available	SUPERFAMILY	54277	CAD & PB1 domains	coord: 76..158
IPR003311	AUX/IAA protein	PANTHER	PTHR31734	AUXIN-RESPONSIVE PROTEIN IAA17	coord: 4..158
IPR000270	PB1 domain	PROSITE	PS51745	PB1	coord: 86..158 score: 21.417372

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002956.1	Lag0002956.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009734	auxin-activated signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding