Lag0002951 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002951
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	chr4: 46914561 .. 46915773 (-)
RNA-Seq Expression	Lag0002951
Synteny	Lag0002951

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCCAAATTAGTCGAGGCTTTCAAATCCCACCCGCTTCACCTCCCCATCCCCGCCAAAGACCTCGACTTCGACTCCCTCAAAGAACTCCCCGATTCCTATGCTTGGATTCAACCCGACTCCTTCCCCTCCCCCAACGATTCCATTTCCCTCTCCGAGTCCATCCCCTTGATCGACCTTTCCCTCCCCAACGCCCCCCACCTCATCGCCAACGCCCTACGAACCTGGGGCGCATTCCAGCTCATCAACCATGCCCTCCCCATCTCCCTCCTTCACTCCCTTGAGTCCTGCGCCGATACCCTCTTCTCCCTCCCCCCTCCTCACAAGCTCAAGGTCGCTCGCTCCCCCGACGGCATCTCCGGCTACGGCATCGTTCGTATCTCCTCCTTCTTCCCTAAACACATGTGGTCCGAAGGTTTCACCATCGTCGGCTCCCCTCGAGACCATTTCCGCCAACTTTGGCCTCACGACTACGCCAAATACTGGTACTTACCACCATATTAATTTAATTATACTATTGCATTCAAATATTCAAATATTCAAATATTCAAATATTCAAATATTCAAATGTTTTTGTAGTGATATAATGGAGGAATATGACCGAGAGATGAAGAGTCTATGTGGAAGGCTGATCTGGATGGCGTTGGGTGAATTGGGCATAACCCGAGAAGACGTCAAATGGGCGGGTCCGAATGGGGATTTCAAGACCAGTCATGCAGCCACCCAGTTGAACTCCTACCCGATTTGTCCGGATCCGGATCGGGCCATGGGGCTCGGGCCCCACACCGACACCAGCCTCTTGACCATCGTCTACCAGAACAACACCAGAGGCTTACAGGTTTTGCGAGAGGGAAACAAGTGGGTCACGGTGGAGCCGGTAGCCGGTGGACTGGTGGTCCAGGTCGGAGACCTGCTCCACATTATGACCAACGGGTTGTTCAAGCCTTCGCTTCATCAAGCGGTGGTGAACCGGACCCGACAACGCATCTCGGTGGCTTACCTTTTCGGGCCACCTGACAACGTGGAAATTTCACCGGTTAAGAAACTTTTGAGCCCAACTCAGCCGCCGATTTACCGTCCGGTAACTTGGACGGAGTACCTCGGTAAAAAGCCGAGCATTTCAACAACGCATTGTCGTCTGTTCGTCTTTGTGCTCCTCTCACTGGAGGACTCTTGGAAGTCAACGATCACAATAATCAAGTCAAAGTAG

mRNA sequence

ATGGCCTCCAAATTAGTCGAGGCTTTCAAATCCCACCCGCTTCACCTCCCCATCCCCGCCAAAGACCTCGACTTCGACTCCCTCAAAGAACTCCCCGATTCCTATGCTTGGATTCAACCCGACTCCTTCCCCTCCCCCAACGATTCCATTTCCCTCTCCGAGTCCATCCCCTTGATCGACCTTTCCCTCCCCAACGCCCCCCACCTCATCGCCAACGCCCTACGAACCTGGGGCGCATTCCAGCTCATCAACCATGCCCTCCCCATCTCCCTCCTTCACTCCCTTGAGTCCTGCGCCGATACCCTCTTCTCCCTCCCCCCTCCTCACAAGCTCAAGGTCGCTCGCTCCCCCGACGGCATCTCCGGCTACGGCATCGTTCGTATCTCCTCCTTCTTCCCTAAACACATGTGGTCCGAAGGTTTCACCATCGTCGGCTCCCCTCGAGACCATTTCCGCCAACTTTGGCCTCACGACTACGCCAAATACTGTGATATAATGGAGGAATATGACCGAGAGATGAAGAGTCTATGTGGAAGGCTGATCTGGATGGCGTTGGGTGAATTGGGCATAACCCGAGAAGACGTCAAATGGGCGGGTCCGAATGGGGATTTCAAGACCAGTCATGCAGCCACCCAGTTGAACTCCTACCCGATTTGTCCGGATCCGGATCGGGCCATGGGGCTCGGGCCCCACACCGACACCAGCCTCTTGACCATCGTCTACCAGAACAACACCAGAGGCTTACAGGTTTTGCGAGAGGGAAACAAGTGGGTCACGGTGGAGCCGGTAGCCGGTGGACTGGTGGTCCAGGTCGGAGACCTGCTCCACATTATGACCAACGGGTTGTTCAAGCCTTCGCTTCATCAAGCGGTGGTGAACCGGACCCGACAACGCATCTCGGTGGCTTACCTTTTCGGGCCACCTGACAACGTGGAAATTTCACCGGTTAAGAAACTTTTGAGCCCAACTCAGCCGCCGATTTACCGTCCGGTAACTTGGACGGAGTACCTCGGTAAAAAGCCGAGCATTTCAACAACGCATTGTCGTCTGTTCGTCTTTGTGCTCCTCTCACTGGAGGACTCTTGGAAGTCAACGATCACAATAATCAAGTCAAAGTAG

Coding sequence (CDS)

ATGGCCTCCAAATTAGTCGAGGCTTTCAAATCCCACCCGCTTCACCTCCCCATCCCCGCCAAAGACCTCGACTTCGACTCCCTCAAAGAACTCCCCGATTCCTATGCTTGGATTCAACCCGACTCCTTCCCCTCCCCCAACGATTCCATTTCCCTCTCCGAGTCCATCCCCTTGATCGACCTTTCCCTCCCCAACGCCCCCCACCTCATCGCCAACGCCCTACGAACCTGGGGCGCATTCCAGCTCATCAACCATGCCCTCCCCATCTCCCTCCTTCACTCCCTTGAGTCCTGCGCCGATACCCTCTTCTCCCTCCCCCCTCCTCACAAGCTCAAGGTCGCTCGCTCCCCCGACGGCATCTCCGGCTACGGCATCGTTCGTATCTCCTCCTTCTTCCCTAAACACATGTGGTCCGAAGGTTTCACCATCGTCGGCTCCCCTCGAGACCATTTCCGCCAACTTTGGCCTCACGACTACGCCAAATACTGTGATATAATGGAGGAATATGACCGAGAGATGAAGAGTCTATGTGGAAGGCTGATCTGGATGGCGTTGGGTGAATTGGGCATAACCCGAGAAGACGTCAAATGGGCGGGTCCGAATGGGGATTTCAAGACCAGTCATGCAGCCACCCAGTTGAACTCCTACCCGATTTGTCCGGATCCGGATCGGGCCATGGGGCTCGGGCCCCACACCGACACCAGCCTCTTGACCATCGTCTACCAGAACAACACCAGAGGCTTACAGGTTTTGCGAGAGGGAAACAAGTGGGTCACGGTGGAGCCGGTAGCCGGTGGACTGGTGGTCCAGGTCGGAGACCTGCTCCACATTATGACCAACGGGTTGTTCAAGCCTTCGCTTCATCAAGCGGTGGTGAACCGGACCCGACAACGCATCTCGGTGGCTTACCTTTTCGGGCCACCTGACAACGTGGAAATTTCACCGGTTAAGAAACTTTTGAGCCCAACTCAGCCGCCGATTTACCGTCCGGTAACTTGGACGGAGTACCTCGGTAAAAAGCCGAGCATTTCAACAACGCATTGTCGTCTGTTCGTCTTTGTGCTCCTCTCACTGGAGGACTCTTGGAAGTCAACGATCACAATAATCAAGTCAAAGTAG

Protein sequence

MASKLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSISLSESIPLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSLCGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSLLTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTRQRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKKPSISTTHCRLFVFVLLSLEDSWKSTITIIKSK

Homology

[Close]

BLAST of Lag0002951 vs. NCBI nr
Match: XP_004135830.1 (gibberellin 3-beta-dioxygenase 1 [Cucumis sativus] >KGN45321.1 hypothetical protein Csa_015611 [Cucumis sativus] >CBX88045.1 gibberellin 3-oxidase, partial [Cucumis sativus])

HSP 1 Score: 584.7 bits (1506), Expect = 5.5e-163
Identity = 274/338 (81.07%), Postives = 306/338 (90.53%), Query Frame = 0


Query: 4   KLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSIS-LSESIPLIDLS 63
           K+ E FKSHP+H+PI  K+LDFDSL ELPDSY WIQPDSFPS + S + LS+SIPLIDLS
Sbjct: 6   KITEVFKSHPVHIPI-HKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLS 65

Query: 64  LPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGISG 123
           LPNAP LI NA RTWGAFQ+INH +PISLLHS+ES A++LFSLPPPHKLK AR  DGISG
Sbjct: 66  LPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISG 125

Query: 124 YGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSLCGRLIW 183
           YG+VRISSFFPK MWSEGFTIVGSP +HFR+LWPHDY +YCDI+EEYDREMKSLCGRL+W
Sbjct: 126 YGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMW 185

Query: 184 MALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSLLTIVYQ 243
           +ALGELGITREDV WAGPNGDFKTS+AATQLNSYP+CPDPDRAMGLG HTDTSLLTIVYQ
Sbjct: 186 VALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQ 245

Query: 244 NNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTRQRISVA 303
           NNTRGLQVLREGN+WVTVEPV G LVVQVGDLLHI+TNGL+   +HQAVVNRTR+R+SVA
Sbjct: 246 NNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVA 305

Query: 304 YLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           Y+FGPP++ EISP+KKLL PTQPP+YR VTWTEYL KK
Sbjct: 306 YVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKK 342

[Close]

BLAST of Lag0002951 vs. NCBI nr
Match: XP_038898776.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 583.6 bits (1503), Expect = 1.2e-162
Identity = 271/340 (79.71%), Postives = 305/340 (89.71%), Query Frame = 0


Query: 4   KLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPS---PNDSISLSESIPLID 63
           K+ E FKSHP+H+P   K+LDFDSL ELPDSY WIQPDSFPS    N+ I LS+S+PLID
Sbjct: 5   KITEVFKSHPVHIP-AHKNLDFDSLHELPDSYDWIQPDSFPSLNLSNNLIPLSDSVPLID 64

Query: 64  LSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGI 123
           LSLPNAPHLI NA RTWGAFQ+INH +PISLLHS+ES A++LFSLPPPHKLK AR PDGI
Sbjct: 65  LSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPPDGI 124

Query: 124 SGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSLCGRL 183
           SGYG+VRISSFFPK MWSEGFTI+GSP +HF++LWPHDY +YCDI+EEYDREMKSLCGRL
Sbjct: 125 SGYGLVRISSFFPKRMWSEGFTILGSPLEHFKKLWPHDYTRYCDIIEEYDREMKSLCGRL 184

Query: 184 IWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSLLTIV 243
           +W+ALGELGITREDV WAG NGDFKT +AATQLNSYP+CPDPDRAMGLG HTDTSLLTIV
Sbjct: 185 MWLALGELGITREDVNWAGLNGDFKTCNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIV 244

Query: 244 YQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTRQRIS 303
           YQNNTRGLQVLREGN+WVTVEPV G LVVQVGDLLHI+TNGL+   +HQAVVNRTR+R+S
Sbjct: 245 YQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLS 304

Query: 304 VAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           VAY+FGPP++ EISP+KKLL PTQPP+YR VTWTEYL KK
Sbjct: 305 VAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKK 343

[Close]

BLAST of Lag0002951 vs. NCBI nr
Match: XP_023514884.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 582.0 bits (1499), Expect = 3.6e-162
Identity = 271/344 (78.78%), Postives = 308/344 (89.53%), Query Frame = 0


Query: 1   MASKLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSISL----SESI 60
           MA+ + + FKS P+H+P   K+LDFDSL ELPDSYAWIQPDSFP+     S+    S+S+
Sbjct: 1   MATTIADVFKSFPVHIP-AHKNLDFDSLHELPDSYAWIQPDSFPTHKHHNSILDSNSDSV 60

Query: 61  PLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARS 120
           PLIDLSLPNA  LI NA R+WGAFQ+INH +PISLL S+ES ADTLFSLPP HKLK AR+
Sbjct: 61  PLIDLSLPNAATLIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAART 120

Query: 121 PDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSL 180
           PDGISGYG+VRISSFFPK MWSEGFTIVGSP DHFRQLWPHDY K+C+I+EEYDREM+SL
Sbjct: 121 PDGISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMRSL 180

Query: 181 CGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSL 240
           CGRL+W+ LGELGITR+D+KWAGP+GDFKTS AATQ NSYP+CPDPDRAMGLGPHTDTSL
Sbjct: 181 CGRLMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDTSL 240

Query: 241 LTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTR 300
           LTIVYQNNTRGLQVLREGN+WVTVEPVAGGLVVQVGDLLHI+TNGL+  +LHQAVVNRTR
Sbjct: 241 LTIVYQNNTRGLQVLREGNRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNRTR 300

Query: 301 QRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           +R+SVAY+FGPP++ EISP+KKLL PTQPP+YRPVTWTEYLGKK
Sbjct: 301 KRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKK 343

[Close]

BLAST of Lag0002951 vs. NCBI nr
Match: XP_023004184.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima] >CAC83089.1 gibberellin 3-oxidase [Cucurbita maxima])

HSP 1 Score: 580.9 bits (1496), Expect = 8.0e-162
Identity = 273/346 (78.90%), Postives = 311/346 (89.88%), Query Frame = 0


Query: 1   MASKLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSP----NDSI--SLSE 60
           MA+ + + FKS P+H+P   K+LDFDSL ELPDSYAWIQPDSFPSP    ++SI  S S+
Sbjct: 1   MATTIADVFKSFPVHIP-AHKNLDFDSLHELPDSYAWIQPDSFPSPTHKHHNSILDSDSD 60

Query: 61  SIPLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVA 120
           S+PLIDLSLPNA  LI NA R+WGAFQ+INH +PISLL S+ES ADTLFSLPP HKLK A
Sbjct: 61  SVPLIDLSLPNAAALIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAA 120

Query: 121 RSPDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMK 180
           R+PDGISGYG+VRISSFFPK MWSEGFTIVGSP DHFRQLWPHDY K+C+I+EEYDREM+
Sbjct: 121 RTPDGISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMR 180

Query: 181 SLCGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDT 240
           SLCGRL+W+ LGELGITR+D+KWAGP+GDFKTS AATQ NSYP+CPDPDRAMGLGPHTDT
Sbjct: 181 SLCGRLMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDT 240

Query: 241 SLLTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNR 300
           SLLTIVYQ+NTRGLQVLREG +WVTVEPVAGGLVVQVGDLLHI+TNGL+  +LHQAVVNR
Sbjct: 241 SLLTIVYQSNTRGLQVLREGKRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNR 300

Query: 301 TRQRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           TR+R+SVAY+FGPP++ EISP+KKLL PTQPP+YRPVTWTEYLGKK
Sbjct: 301 TRKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKK 345

[Close]

BLAST of Lag0002951 vs. NCBI nr
Match: KAG7025755.1 (Gibberellin 3-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 580.5 bits (1495), Expect = 1.0e-161
Identity = 270/344 (78.49%), Postives = 307/344 (89.24%), Query Frame = 0


Query: 1   MASKLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSISL----SESI 60
           MA+ + + FKS P+H+P   K+ DFDSL ELPDSYAWIQPDSFP+     S+    S+S+
Sbjct: 16  MATTIADVFKSFPVHIP-AHKNFDFDSLHELPDSYAWIQPDSFPTHKHHNSILDSDSDSV 75

Query: 61  PLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARS 120
           PLIDLSLPNA  LI NA R+WGAFQ+INH +PISLL S+ES ADTLFSLPP HKLK AR+
Sbjct: 76  PLIDLSLPNAATLIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAART 135

Query: 121 PDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSL 180
           PDGISGYG+VRISSFFPK MWSEGFTIVGSP DHFRQLWPHDY K+C+I+EEYDREM+SL
Sbjct: 136 PDGISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMRSL 195

Query: 181 CGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSL 240
           CGRL+W+ LGELGITR+D+KWAGP+GDFKTS AATQ NSYP+CPDPDRAMGLGPHTDTSL
Sbjct: 196 CGRLMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDTSL 255

Query: 241 LTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTR 300
           LTIVYQNNTRGLQVLREGN+WVTVEPVAGGLVVQVGDLLHI+TNGL+  +LHQAVVNRTR
Sbjct: 256 LTIVYQNNTRGLQVLREGNRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNRTR 315

Query: 301 QRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           +R+SVAY+FGPP++ EISP+KKLL PTQPP+YRPVTWTEYLGKK
Sbjct: 316 KRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKK 358

[Close]

BLAST of Lag0002951 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 2.4e-116
Identity = 202/372 (54.30%), Postives = 261/372 (70.16%), Query Frame = 0


Query: 5   LVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSISLSES---IPLIDL 64
           L EA+++HP+H  +  K  DF+SL+ELP+SY W   D     + +  + ES   +P+IDL
Sbjct: 4   LSEAYRAHPVH--VNHKHPDFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDL 63

Query: 65  SLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGIS 124
           + PNA  LI  A +TWG +Q++NH +P+SLL  ++    TLFSLP   K K  RSPDG+S
Sbjct: 64  NDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVS 123

Query: 125 GYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSLCGRLI 184
           GYGI RISSFFPK MW EGFTIVGSP DHFR+LWP DY ++CDI+ +YD  MK L G L+
Sbjct: 124 GYGIARISSFFPKLMWYEGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLM 183

Query: 185 WMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSLLTIVY 244
            + L  LGIT+ED+KWAG    F+ + AA QLNSYP CPDPD AMGL PHTD++ LTI+ 
Sbjct: 184 CLMLDSLGITKEDIKWAGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILS 243

Query: 245 QNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTRQRISV 304
           QN+  GLQV REG+ W+TV P+ GGLVV VGDL HI++NGL+   LH+ +VNRTRQR SV
Sbjct: 244 QNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQRFSV 303

Query: 305 AYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK-----PSISTTHCRLFVFVLL 364
           AYL+GPP NVEI P  KL+ PT+PP+YR VTW EYLG K      ++S+      +  L 
Sbjct: 304 AYLYGPPSNVEICPHAKLIGPTKPPLYRSVTWNEYLGTKAKHFNKALSSVRLCTPINGLF 363

Query: 365 SLEDSWKSTITI 369
            + DS K+++ +
Sbjct: 364 DVNDSNKNSVQV 373

[Close]

BLAST of Lag0002951 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 399.4 bits (1025), Expect = 4.3e-110
Identity = 189/345 (54.78%), Postives = 242/345 (70.14%), Query Frame = 0


Query: 1   MASKLVEAFKSHPLHLP---IPAKDLDFDSLKELPDSYAWIQPDS--FPSPNDSISLSES 60
           M + L + F+ HP+HLP   IP    DF SL+ELPDSY W   D   F +     +  E+
Sbjct: 1   MPAMLTDVFRGHPIHLPHSHIP----DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGEN 60

Query: 61  IPLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVAR 120
           IPLIDL  P+A + I +A RTWGAFQ+ NH +P+ LL  +E    +LF LP   KLK AR
Sbjct: 61  IPLIDLDHPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSAR 120

Query: 121 SPDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKS 180
           S  G+SGYG+ RI+SFF K MWSEGFTI GSP + FR+LWP  +  YCDI+EEY+  MK 
Sbjct: 121 SETGVSGYGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKK 180

Query: 181 LCGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTS 240
           L  +L+W+AL  LG++ ED++WA  + D   + AA QLN YP+CP+PDRAMGL  HTD++
Sbjct: 181 LASKLMWLALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDST 240

Query: 241 LLTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRT 300
           LLTI+YQNNT GLQV R+   WVTV P  G LVV VGDL HI++NGLFK  LH+A VN+T
Sbjct: 241 LLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQT 300

Query: 301 RQRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           R R+SVA+L+GP  +++ISPV KL+SP + P+Y+ VTW EYL  K
Sbjct: 301 RARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTK 341

[Close]

BLAST of Lag0002951 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 385.2 bits (988), Expect = 8.5e-106
Identity = 181/345 (52.46%), Postives = 237/345 (68.70%), Query Frame = 0


Query: 1   MASKLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSISLS-----ES 60
           M+S L + F+SHP+H+P+ +   DF   K LPDSY W       +P D +  S     E+
Sbjct: 1   MSSTLSDVFRSHPIHIPL-SNPPDF---KSLPDSYTW-------TPKDDLLFSASASDET 60

Query: 61  IPLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVAR 120
           +PLIDLS  +   L+ +A  TWGAFQ+ NH +P  LL  +E    +LF LP   KLK AR
Sbjct: 61  LPLIDLSDIHVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAAR 120

Query: 121 SPDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKS 180
           S +G+SGYG+ RI+SFF K MWSEGFT++GSP   FR+LWP  + KYC+I+EEY+  M+ 
Sbjct: 121 SENGVSGYGVARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQK 180

Query: 181 LCGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTS 240
           L  +L+W ALG LG+  +D++WAGPN DF+ + A  QLN YP CP+PDRAMGL  HTD++
Sbjct: 181 LAAKLMWFALGSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDST 240

Query: 241 LLTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRT 300
           L+TI+YQNNT GLQV R+   WVT  PV G LVV VGDLLHI+TNG+F   LH+A VN  
Sbjct: 241 LMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV 300

Query: 301 RQRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           R R S+AYL+GPP ++ ISP+ KL+ P Q P+Y  +TW +YL  K
Sbjct: 301 RSRFSMAYLWGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATK 334

[Close]

BLAST of Lag0002951 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.0e-90
Identity = 164/320 (51.25%), Postives = 217/320 (67.81%), Query Frame = 0


Query: 27  SLKELPDSYAW--IQPDSFPSPNDSIS---LSESIPLIDLSLPNAPHLIANALRTWGAFQ 86
           SL+ LP+S+ W     DS   P+ ++S   + ESIP+IDLS P+   LI +A +TWGAFQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 87  LINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGISGYGIVRISSFFPKHMWSEGF 146
           + NH +   LL  +ES + TLF +P   KL+ A S  G+SGYG  RIS FF K MWSEGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 147 TIV-GSPRDHFRQLWPHDYAKYCDIMEEYDREMKSLCGRLIWMALGELGITREDVKWAG- 206
           TI   S R+HF  LWPHD+ KYC I++EY  EM+ L  RL++  LG LG+T ED++WA  
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194

Query: 207 -PNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSLLTIVYQNNTRGLQVLREGNKWV 266
                 K    A +LN YP+CP+P+RAMGL  HTD+++LTI++Q+NT GLQV RE + WV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254

Query: 267 TVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTRQRISVAYLF-GPPDNVEISPVK 326
           TVEP  G LVV +GDL HI++NG     +H+A VN TR RIS+AYL+ GP  +V+I+P+ 
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314

Query: 327 KLLSPTQPPIYRPVTWTEYL 338
           KL  P +P +YR +TW EYL
Sbjct: 315 KLTGPAEPSLYRSITWKEYL 334

[Close]

BLAST of Lag0002951 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.6e-83
Identity = 158/347 (45.53%), Postives = 218/347 (62.82%), Query Frame = 0


Query: 3   SKLVEAFKSHPLHLP--IPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSISLSESIPLID 62
           S + + FK++P++    IP   LDF + K LPDS+ W +P       +  + S  IP+I 
Sbjct: 2   SSVTQLFKNNPVNRDRIIP---LDFTNTKTLPDSHVWSKP-------EPETTSGPIPVIS 61

Query: 63  LSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGI 122
           LS P    L+  A   WG F + +H +  SLLH+++     LFSLP   K+   RSPD  
Sbjct: 62  LSNPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDES 121

Query: 123 SGYGIVRISSFFPKHMWSEGFTIVGSP-RDHFRQLWPHDYAKYCDIMEEYDREMKSLCGR 182
           +GYG+VRIS F+ K MWSEGF+++GS  R H   LWP D+A++C++MEEY + M  L  R
Sbjct: 122 TGYGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHR 181

Query: 183 LIWMALGELGITREDVKWAGPN---GDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSL 242
           LI M +G LG+T ED+ W  P+       +  +  QLNSYP+CPDP  AMGL PHTD+SL
Sbjct: 182 LISMLMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSL 241

Query: 243 LTIVYQNNTRGLQV---LREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVN 302
           LTI+YQ N  GL++     EG++W+ VEP+ G LVV +GDL HI++NG F+ ++H+AVVN
Sbjct: 242 LTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVN 301

Query: 303 RTRQRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           +T  R+S AY  GPP N++I P+        PPIYR + W EYL  K
Sbjct: 302 KTHHRVSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAK 336

[Close]

BLAST of Lag0002951 vs. ExPASy TrEMBL
Match: K7ZTU0 (Gibberellin 3-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga3ox1 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.7e-163
Identity = 274/338 (81.07%), Postives = 306/338 (90.53%), Query Frame = 0


Query: 4   KLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSIS-LSESIPLIDLS 63
           K+ E FKSHP+H+PI  K+LDFDSL ELPDSY WIQPDSFPS + S + LS+SIPLIDLS
Sbjct: 6   KITEVFKSHPVHIPI-HKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPLIDLS 65

Query: 64  LPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGISG 123
           LPNAP LI NA RTWGAFQ+INH +PISLLHS+ES A++LFSLPPPHKLK AR  DGISG
Sbjct: 66  LPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSDGISG 125

Query: 124 YGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSLCGRLIW 183
           YG+VRISSFFPK MWSEGFTIVGSP +HFR+LWPHDY +YCDI+EEYDREMKSLCGRL+W
Sbjct: 126 YGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCGRLMW 185

Query: 184 MALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSLLTIVYQ 243
           +ALGELGITREDV WAGPNGDFKTS+AATQLNSYP+CPDPDRAMGLG HTDTSLLTIVYQ
Sbjct: 186 VALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQ 245

Query: 244 NNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTRQRISVA 303
           NNTRGLQVLREGN+WVTVEPV G LVVQVGDLLHI+TNGL+   +HQAVVNRTR+R+SVA
Sbjct: 246 NNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVA 305

Query: 304 YLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           Y+FGPP++ EISP+KKLL PTQPP+YR VTWTEYL KK
Sbjct: 306 YVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKK 342

[Close]

BLAST of Lag0002951 vs. ExPASy TrEMBL
Match: Q8GT57 (Gibberellin 3-oxidase OS=Cucurbita maxima OX=3661 GN=ga3ox PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.9e-162
Identity = 273/346 (78.90%), Postives = 311/346 (89.88%), Query Frame = 0


Query: 1   MASKLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSP----NDSI--SLSE 60
           MA+ + + FKS P+H+P   K+LDFDSL ELPDSYAWIQPDSFPSP    ++SI  S S+
Sbjct: 1   MATTIADVFKSFPVHIP-AHKNLDFDSLHELPDSYAWIQPDSFPSPTHKHHNSILDSDSD 60

Query: 61  SIPLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVA 120
           S+PLIDLSLPNA  LI NA R+WGAFQ+INH +PISLL S+ES ADTLFSLPP HKLK A
Sbjct: 61  SVPLIDLSLPNAAALIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAA 120

Query: 121 RSPDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMK 180
           R+PDGISGYG+VRISSFFPK MWSEGFTIVGSP DHFRQLWPHDY K+C+I+EEYDREM+
Sbjct: 121 RTPDGISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMR 180

Query: 181 SLCGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDT 240
           SLCGRL+W+ LGELGITR+D+KWAGP+GDFKTS AATQ NSYP+CPDPDRAMGLGPHTDT
Sbjct: 181 SLCGRLMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDT 240

Query: 241 SLLTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNR 300
           SLLTIVYQ+NTRGLQVLREG +WVTVEPVAGGLVVQVGDLLHI+TNGL+  +LHQAVVNR
Sbjct: 241 SLLTIVYQSNTRGLQVLREGKRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNR 300

Query: 301 TRQRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           TR+R+SVAY+FGPP++ EISP+KKLL PTQPP+YRPVTWTEYLGKK
Sbjct: 301 TRKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKK 345

[Close]

BLAST of Lag0002951 vs. ExPASy TrEMBL
Match: A0A6J1H974 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460769 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.9e-161
Identity = 269/344 (78.20%), Postives = 307/344 (89.24%), Query Frame = 0


Query: 1   MASKLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSISL----SESI 60
           MA+ + + FKS P+H+P   K+LDFDSL ELPDSYAWIQPDSFP+     S+    ++S+
Sbjct: 1   MATTIADVFKSFPVHIP-AHKNLDFDSLHELPDSYAWIQPDSFPTHKHHNSILDSDADSV 60

Query: 61  PLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARS 120
           PLIDLSLPNA  LI NA R+WGAFQ+INH +PISLL S+ES ADTLFSLPP HKLK AR+
Sbjct: 61  PLIDLSLPNAATLIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAART 120

Query: 121 PDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSL 180
           PDGISGYG+VRISSFFPK MWSEGFTIVGSP DHFRQLWPHDY K+C+I+EEYDREM+SL
Sbjct: 121 PDGISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMRSL 180

Query: 181 CGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSL 240
           CGRL+W+ LGELGITR+D+KWAGP+GDFKTS AATQ NSYP+CPDPDRAMGLGPHTDTSL
Sbjct: 181 CGRLMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDTSL 240

Query: 241 LTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTR 300
           LTIVYQNNTRGLQVLREG +WVTVEPVAGGLVVQVGDLLHI+TNGL+  +LHQAVVNRTR
Sbjct: 241 LTIVYQNNTRGLQVLREGKRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNRTR 300

Query: 301 QRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           +R+SVAY+FGPP++ EISP+KKLL PTQPP+YRPVTWTEYLGKK
Sbjct: 301 KRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKK 343

[Close]

BLAST of Lag0002951 vs. ExPASy TrEMBL
Match: A0A1S3CDD3 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103499758 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 5.6e-161
Identity = 270/340 (79.41%), Postives = 304/340 (89.41%), Query Frame = 0


Query: 4   KLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSIS---LSESIPLID 63
           K+ E FKSHP+H+PI  K+ DFDSL+ELPDSY WIQPDSF  PN S+S   LS+SIPLID
Sbjct: 6   KITEVFKSHPVHIPI-HKNFDFDSLQELPDSYDWIQPDSF--PNSSLSNNHLSDSIPLID 65

Query: 64  LSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGI 123
           LSLPNAPHLI NA RTWGAFQ+INH +PISLLHS+ES A++LFSLP PHKLK AR  DGI
Sbjct: 66  LSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGI 125

Query: 124 SGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSLCGRL 183
           SGYG+VRISSFFPK MWSEGFTIVGSP +HFR+LWPHDY +YCDI+EEYD+EMKSLCGRL
Sbjct: 126 SGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRL 185

Query: 184 IWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSLLTIV 243
           +W+ALGELGITREDV WAGPNGDF TS+AATQ NSYP+CPDP+RAMGLG HTDTSLLTIV
Sbjct: 186 MWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIV 245

Query: 244 YQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTRQRIS 303
           YQNNTRGLQVLREGN+WVTVEPV G LVVQVGDLLHI+TNGL+   +HQAVVNRTR+R+S
Sbjct: 246 YQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLS 305

Query: 304 VAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           VAY+FGPP++ EISP+KKLL PTQPP+YR VTWTEYL KK
Sbjct: 306 VAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKK 342

[Close]

BLAST of Lag0002951 vs. ExPASy TrEMBL
Match: A0A5A7US98 (Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002410 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 9.6e-161
Identity = 270/340 (79.41%), Postives = 303/340 (89.12%), Query Frame = 0


Query: 4   KLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSIS---LSESIPLID 63
           K+ E FKSHP+H+PI  K+ DFDSL ELPDSY WIQPDSF  PN S+S   LS+SIPLID
Sbjct: 6   KITEVFKSHPVHIPI-HKNFDFDSLHELPDSYDWIQPDSF--PNSSLSNNHLSDSIPLID 65

Query: 64  LSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGI 123
           LSLPNAPHLI NA RTWGAFQ+INH +PISLLHS+ES A++LFSLP PHKLK AR  DGI
Sbjct: 66  LSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGI 125

Query: 124 SGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKSLCGRL 183
           SGYG+VRISSFFPK MWSEGFTIVGSP +HFR+LWPHDY +YCDI+EEYD+EMKSLCGRL
Sbjct: 126 SGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRL 185

Query: 184 IWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSLLTIV 243
           +W+ALGELGITREDV WAGPNGDF TS+AATQ NSYP+CPDP+RAMGLG HTDTSLLTIV
Sbjct: 186 MWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIV 245

Query: 244 YQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTRQRIS 303
           YQNNTRGLQVLREGN+WVTVEPV G LVVQVGDLLHI+TNGL+   +HQAVVNRTR+R+S
Sbjct: 246 YQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLS 305

Query: 304 VAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           VAY+FGPP++ EISP+KKLL PTQPP+YR VTWTEYL KK
Sbjct: 306 VAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKK 342

[Close]

BLAST of Lag0002951 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 399.4 bits (1025), Expect = 3.1e-111
Identity = 189/345 (54.78%), Postives = 242/345 (70.14%), Query Frame = 0


Query: 1   MASKLVEAFKSHPLHLP---IPAKDLDFDSLKELPDSYAWIQPDS--FPSPNDSISLSES 60
           M + L + F+ HP+HLP   IP    DF SL+ELPDSY W   D   F +     +  E+
Sbjct: 1   MPAMLTDVFRGHPIHLPHSHIP----DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGEN 60

Query: 61  IPLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVAR 120
           IPLIDL  P+A + I +A RTWGAFQ+ NH +P+ LL  +E    +LF LP   KLK AR
Sbjct: 61  IPLIDLDHPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSAR 120

Query: 121 SPDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKS 180
           S  G+SGYG+ RI+SFF K MWSEGFTI GSP + FR+LWP  +  YCDI+EEY+  MK 
Sbjct: 121 SETGVSGYGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKK 180

Query: 181 LCGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTS 240
           L  +L+W+AL  LG++ ED++WA  + D   + AA QLN YP+CP+PDRAMGL  HTD++
Sbjct: 181 LASKLMWLALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDST 240

Query: 241 LLTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRT 300
           LLTI+YQNNT GLQV R+   WVTV P  G LVV VGDL HI++NGLFK  LH+A VN+T
Sbjct: 241 LLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQT 300

Query: 301 RQRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           R R+SVA+L+GP  +++ISPV KL+SP + P+Y+ VTW EYL  K
Sbjct: 301 RARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTK 341

[Close]

BLAST of Lag0002951 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 385.2 bits (988), Expect = 6.0e-107
Identity = 181/345 (52.46%), Postives = 237/345 (68.70%), Query Frame = 0


Query: 1   MASKLVEAFKSHPLHLPIPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSISLS-----ES 60
           M+S L + F+SHP+H+P+ +   DF   K LPDSY W       +P D +  S     E+
Sbjct: 1   MSSTLSDVFRSHPIHIPL-SNPPDF---KSLPDSYTW-------TPKDDLLFSASASDET 60

Query: 61  IPLIDLSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVAR 120
           +PLIDLS  +   L+ +A  TWGAFQ+ NH +P  LL  +E    +LF LP   KLK AR
Sbjct: 61  LPLIDLSDIHVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAAR 120

Query: 121 SPDGISGYGIVRISSFFPKHMWSEGFTIVGSPRDHFRQLWPHDYAKYCDIMEEYDREMKS 180
           S +G+SGYG+ RI+SFF K MWSEGFT++GSP   FR+LWP  + KYC+I+EEY+  M+ 
Sbjct: 121 SENGVSGYGVARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQK 180

Query: 181 LCGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTS 240
           L  +L+W ALG LG+  +D++WAGPN DF+ + A  QLN YP CP+PDRAMGL  HTD++
Sbjct: 181 LAAKLMWFALGSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDST 240

Query: 241 LLTIVYQNNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRT 300
           L+TI+YQNNT GLQV R+   WVT  PV G LVV VGDLLHI+TNG+F   LH+A VN  
Sbjct: 241 LMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV 300

Query: 301 RQRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           R R S+AYL+GPP ++ ISP+ KL+ P Q P+Y  +TW +YL  K
Sbjct: 301 RSRFSMAYLWGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATK 334

[Close]

BLAST of Lag0002951 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 335.1 bits (858), Expect = 7.1e-92
Identity = 164/320 (51.25%), Postives = 217/320 (67.81%), Query Frame = 0


Query: 27  SLKELPDSYAW--IQPDSFPSPNDSIS---LSESIPLIDLSLPNAPHLIANALRTWGAFQ 86
           SL+ LP+S+ W     DS   P+ ++S   + ESIP+IDLS P+   LI +A +TWGAFQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 87  LINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGISGYGIVRISSFFPKHMWSEGF 146
           + NH +   LL  +ES + TLF +P   KL+ A S  G+SGYG  RIS FF K MWSEGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 147 TIV-GSPRDHFRQLWPHDYAKYCDIMEEYDREMKSLCGRLIWMALGELGITREDVKWAG- 206
           TI   S R+HF  LWPHD+ KYC I++EY  EM+ L  RL++  LG LG+T ED++WA  
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194

Query: 207 -PNGDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSLLTIVYQNNTRGLQVLREGNKWV 266
                 K    A +LN YP+CP+P+RAMGL  HTD+++LTI++Q+NT GLQV RE + WV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254

Query: 267 TVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVNRTRQRISVAYLF-GPPDNVEISPVK 326
           TVEP  G LVV +GDL HI++NG     +H+A VN TR RIS+AYL+ GP  +V+I+P+ 
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314

Query: 327 KLLSPTQPPIYRPVTWTEYL 338
           KL  P +P +YR +TW EYL
Sbjct: 315 KLTGPAEPSLYRSITWKEYL 334

[Close]

BLAST of Lag0002951 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 311.2 bits (796), Expect = 1.1e-84
Identity = 158/347 (45.53%), Postives = 218/347 (62.82%), Query Frame = 0


Query: 3   SKLVEAFKSHPLHLP--IPAKDLDFDSLKELPDSYAWIQPDSFPSPNDSISLSESIPLID 62
           S + + FK++P++    IP   LDF + K LPDS+ W +P       +  + S  IP+I 
Sbjct: 2   SSVTQLFKNNPVNRDRIIP---LDFTNTKTLPDSHVWSKP-------EPETTSGPIPVIS 61

Query: 63  LSLPNAPHLIANALRTWGAFQLINHALPISLLHSLESCADTLFSLPPPHKLKVARSPDGI 122
           LS P    L+  A   WG F + +H +  SLLH+++     LFSLP   K+   RSPD  
Sbjct: 62  LSNPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDES 121

Query: 123 SGYGIVRISSFFPKHMWSEGFTIVGSP-RDHFRQLWPHDYAKYCDIMEEYDREMKSLCGR 182
           +GYG+VRIS F+ K MWSEGF+++GS  R H   LWP D+A++C++MEEY + M  L  R
Sbjct: 122 TGYGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHR 181

Query: 183 LIWMALGELGITREDVKWAGPN---GDFKTSHAATQLNSYPICPDPDRAMGLGPHTDTSL 242
           LI M +G LG+T ED+ W  P+       +  +  QLNSYP+CPDP  AMGL PHTD+SL
Sbjct: 182 LISMLMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSL 241

Query: 243 LTIVYQNNTRGLQV---LREGNKWVTVEPVAGGLVVQVGDLLHIMTNGLFKPSLHQAVVN 302
           LTI+YQ N  GL++     EG++W+ VEP+ G LVV +GDL HI++NG F+ ++H+AVVN
Sbjct: 242 LTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVN 301

Query: 303 RTRQRISVAYLFGPPDNVEISPVKKLLSPTQPPIYRPVTWTEYLGKK 341
           +T  R+S AY  GPP N++I P+        PPIYR + W EYL  K
Sbjct: 302 KTHHRVSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAK 336

[Close]

BLAST of Lag0002951 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.2e-43
Identity = 98/298 (32.89%), Postives = 154/298 (51.68%), Query Frame = 0


Query: 52  LSESIPLIDLSLPNAPH---------LIANALRTWGAFQLINHALPISLLHSLESCADTL 111
           L   IP+IDLS  + P           ++ A   WG FQ+INH + + ++  +E  A   
Sbjct: 51  LHHQIPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEF 110

Query: 112 FSLPPPHKLKVARSPDGISGYGIVRISSFFPKHMWSEGFTI-VGSPRDHFRQLWPHDYAK 171
           F +P   K K    P  + GYG   I S   K  W   F + V  P+    +LWP   A+
Sbjct: 111 FDMPLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPAR 170

Query: 172 YCDIMEEYDREMKSLCGRLIWMALGELGITREDVKWAGPNGDFKTSHAATQLNSYPICPD 231
           + + +E Y +E++ LC RL+      LG+  E  +       F  +  A ++N YP C  
Sbjct: 171 FSESLEGYSKEIRELCKRLLKYIAISLGLKEERFEEM-----FGEAVQAVRMNYYPPCSS 230

Query: 232 PDRAMGLGPHTDTSLLTIVYQ--NNTRGLQVLREGNKWVTVEPVAGGLVVQVGDLLHIMT 291
           PD  +GL PH+D S LT++ Q  N+  GLQ+L++ N WV V+P+   LV+ +GD + +++
Sbjct: 231 PDLVLGLSPHSDGSALTVLQQSKNSCVGLQILKD-NTWVPVKPLPNALVINIGDTIEVLS 290

Query: 292 NGLFKPSLHQAVVNRTRQRISVAYLFGPPDNVEISPVKKLL-SPTQPPIYRPVTWTEY 337
           NG +K   H+AV NR ++R+++   + P   VEI P+ +L+   T P  YR     +Y
Sbjct: 291 NGKYKSVEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDY 342

The following BLAST results are available for this feature:

BLAST of Lag0002951 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135830.1	5.5e-163	81.07	gibberellin 3-beta-dioxygenase 1 [Cucumis sativus] >KGN45321.1 hypothetical prot...	[more]
XP_038898776.1	1.2e-162	79.71	gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida]	[more]
XP_023514884.1	3.6e-162	78.78	gibberellin 3-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023004184.1	8.0e-162	78.90	gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima] >CAC83089.1 gibberellin...	[more]
KAG7025755.1	1.0e-161	78.49	Gibberellin 3-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of Lag0002951 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	2.4e-116	54.30	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q39103	4.3e-110	54.78	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9ZT84	8.5e-106	52.46	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q9C971	1.0e-90	51.25	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9SVS8	1.6e-83	45.53	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]

BLAST of Lag0002951 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
K7ZTU0	2.7e-163	81.07	Gibberellin 3-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga3ox1 PE=2 SV=1	[more]
Q8GT57	3.9e-162	78.90	Gibberellin 3-oxidase OS=Cucurbita maxima OX=3661 GN=ga3ox PE=2 SV=1	[more]
A0A6J1H974	1.9e-161	78.20	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460...	[more]
A0A1S3CDD3	5.6e-161	79.41	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103499758 PE...	[more]
A0A5A7US98	9.6e-161	79.41	Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of Lag0002951 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15550.1	3.1e-111	54.78	gibberellin 3-oxidase 1	[more]
AT1G80340.1	6.0e-107	52.46	gibberellin 3-oxidase 2	[more]
AT1G80330.1	7.1e-92	51.25	gibberellin 3-oxidase 4	[more]
AT4G21690.1	1.1e-84	45.53	gibberellin 3-oxidase 3	[more]
AT3G21420.1	1.2e-43	32.89	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 20..344 e-value: 5.6E-76 score: 257.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 56..157 e-value: 4.0E-14 score: 53.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 211..306 e-value: 3.0E-24 score: 85.4
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 2..349
None	No IPR available	PANTHER	PTHR47991:SF87	GIBBERELLIN 3-BETA-DIOXYGENASE 1-LIKE	coord: 2..349
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 20..340
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 206..307 score: 12.502746

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002951.1	Lag0002951.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding