Lag0002949 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002949
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr4: 46888292 .. 46890697 (+)
RNA-Seq Expression	Lag0002949
Synteny	Lag0002949

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCCCAAGCAAAAGCCAAAATCCCCACAATAGATCTGTCCAAAGAGAAATTGAAGGCAGCTGGCACAGAAGAATGGGCCTTGAGCTGCAAAGAGATGAGAGTTGCTTTAGAAGAATTTGGTTGCTTTTTGGTGGTTTATGATGAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTCTTTGATCTGCCATTAGAGAGTAGGATTCAAAACACGAGTGAGAAGCCTTATCATGGCTACTTTGGTCGATATTCATTTCTTCCTCTCTATGAATCCATGGCCATTGATAATCCTACTGACCTTCAAGCAGCTCAAGCTTTTGCCAATCTCATGTGGCCTGCTGGAAATAATCACTTCTGGTAATTAATATATTATTGTTCTTTGTTTAGTTTTATTTTACTTCATTTATTGCTTAAACTCCACAAATCATTCCTAAACGTTTTAAGAATATTGCATCAGATGTAGAGGTGGGGATGATCTAATAATTTTATAAGTTTTAATTTAGTTCTTGTGATTTTAGTTTCATTTTCCTTCTCATGATTTTAAAATTTTCAATTTGGTTCTTATGATTTGGTCAAACCCAATAAACTAATTTTTTTTATTAGAAATATTATTTTTTTGAAGTATTGATAAAAGATTAAATTTACCTATTCAAACTTTTGGCAGTTGACTCATCATAAATCAACCCAAAAGTTTAAGCTGATAGGTAATGATTAGTTTAACACTCTCCCTCATTTATTTCATGTTCAAACCTTTGTAACGTCGTTTATTCTCCAATTAATATTGATTTCCACTAATTGGATTTTCTACAAAAATTTTCAAGCTCATAAGTGAAAGAAAATATTAAATTTACTATTAACCTATCAACTTAAGATTTTGGGTTCATCGGTGACTTAACAGGTTCGAACACAAGACCTTTTGGCCTAATACCTGTTAAATAATCAATCAACTCAAAAGCATAAGCTTTTGATTTCATTAGTAATTTGACAACACAACTATATCACATGTGAGGTAAGAGAATTTGAACCTTACTCTCGGTTGGAAGCAGAAATGTCTTACTTGATCAACTATCCTCGTGTTGGCATTTCCTAGACAATTTTTTACACATTCAAAGAAACCCATTCATTTTTTTTTTTTGGTACTAAACCCAAATCCTAGAACAATCAATCCAACAAAAAATCAGCTCAGCTCATTGCCACAATGATCAAAAACATAATTTATCTTCTTAACAATTGAACTTTAACTACATCTAAAAGGGGCCAAATTAATGTCAATTATTTCTAACATTTTTGCTTTTCAAAAGTGAAAAGTTGGGTGTTTGTTTGATTCCCTTCCCTGAAACTGAACCAGTGAAACTGTTCAAAATTTCTCAACCATGGTGGCGAAACTGGACGAGATGGTAACAAGAATGGTGTTCGAAAGCTATGGAGTACCGAAGCTGAGTGACTCAAACATCGAATCCACCAACCATCTTCTTCGATGCTTCAAATACAGAGTTCCTGAGAAAGACGAAACCGATCTGGGTTTGCACTCTCATACAGATCTGACTTTCTTTTCCATAATTCATCAGCATCTCATCGGAGGTCTGCAAATACAATCACTTGATGATGAATGGATCGATGTCGAGCCCTCGGGGCACTGCTCGTTTATCGTCATGGCTGGCGATGCAATCATGGTACGAACATTCTCGAAGATAGATCTTTCAACTTTAAAAAATGTCTAATCAATCTTTAAGTTTTCAACCATGTTGATAATAGGTCATCGATTTTAGTATAGTTTTTGAAATTTTAATATTGCATCTAATAGGCCTATGATATATTTGACGATTTTTTAAATTCACAGGCCTAATTTAGTCCTATTCTTTTTAATTCTCTATCAGATATGTTTACAAATTCTTAAAAATGTTGAATAGGTTGGAAACATATTAAATACATAACTAAGAGTTTGTGAGACCTATTAAATAGAGTATAACTGAAAGTTTGTAATTTAACGTATATATATTTTAGTTTTTATTTGATTTGGTTTTATAAAGAACTTAACTTAAACCCAAATCAATATTTAAAAAATAAAATTGAAACTTTTGAAACATAAAAAGAAAAAAGAAAAAAGAAAAGGGATTTAGAAGGTTTTCATCGATGGGTGTAGGGTTGGAGCAACGAGAGGATAAGGGCTTGCAGGCACCGAGTGATAATGAGAGGCAACGAAAGAAGATACTCAGTTGGGTTATTTTCGTTCAAAAGTGGAACAATTGGTGTGCCTGAGGAACTTGTGGACGACGCCAACCCACTGCGATACAAGCCCTTTGATCACTTCCAGTTCCTTAGCTATGACAAAGCCAATGCTTCCCATAAAACTGTCTCTCGTATTAAAGATTATTGCGGCCTTTGA

mRNA sequence

ATGGGTTCCCAAGCAAAAGCCAAAATCCCCACAATAGATCTGTCCAAAGAGAAATTGAAGGCAGCTGGCACAGAAGAATGGGCCTTGAGCTGCAAAGAGATGAGAGTTGCTTTAGAAGAATTTGGTTGCTTTTTGGTGGTTTATGATGAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTCTTTGATCTGCCATTAGAGAGTAGGATTCAAAACACGAGTGAGAAGCCTTATCATGGCTACTTTGGTCGATATTCATTTCTTCCTCTCTATGAATCCATGGCCATTGATAATCCTACTGACCTTCAAGCAGCTCAAGCTTTTGCCAATCTCATGTGGCCTGCTGGAAATAATCACTTCTGTGAAACTGTTCAAAATTTCTCAACCATGGTGGCGAAACTGGACGAGATGGTAACAAGAATGGTGTTCGAAAGCTATGGAGTACCGAAGCTGAGTGACTCAAACATCGAATCCACCAACCATCTTCTTCGATGCTTCAAATACAGAGTTCCTGAGAAAGACGAAACCGATCTGGGTTTGCACTCTCATACAGATCTGACTTTCTTTTCCATAATTCATCAGCATCTCATCGGAGGTCTGCAAATACAATCACTTGATGATGAATGGATCGATGTCGAGCCCTCGGGGCACTGCTCGTTTATCGTCATGGCTGGCGATGCAATCATGGGTTGGAGCAACGAGAGGATAAGGGCTTGCAGGCACCGAGTGATAATGAGAGGCAACGAAAGAAGATACTCAGTTGGGTTATTTTCGTTCAAAAGTGGAACAATTGGTGTGCCTGAGGAACTTGTGGACGACGCCAACCCACTGCGATACAAGCCCTTTGATCACTTCCAGTTCCTTAGCTATGACAAAGCCAATGCTTCCCATAAAACTGTCTCTCGTATTAAAGATTATTGCGGCCTTTGA

Coding sequence (CDS)

ATGGGTTCCCAAGCAAAAGCCAAAATCCCCACAATAGATCTGTCCAAAGAGAAATTGAAGGCAGCTGGCACAGAAGAATGGGCCTTGAGCTGCAAAGAGATGAGAGTTGCTTTAGAAGAATTTGGTTGCTTTTTGGTGGTTTATGATGAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTCTTTGATCTGCCATTAGAGAGTAGGATTCAAAACACGAGTGAGAAGCCTTATCATGGCTACTTTGGTCGATATTCATTTCTTCCTCTCTATGAATCCATGGCCATTGATAATCCTACTGACCTTCAAGCAGCTCAAGCTTTTGCCAATCTCATGTGGCCTGCTGGAAATAATCACTTCTGTGAAACTGTTCAAAATTTCTCAACCATGGTGGCGAAACTGGACGAGATGGTAACAAGAATGGTGTTCGAAAGCTATGGAGTACCGAAGCTGAGTGACTCAAACATCGAATCCACCAACCATCTTCTTCGATGCTTCAAATACAGAGTTCCTGAGAAAGACGAAACCGATCTGGGTTTGCACTCTCATACAGATCTGACTTTCTTTTCCATAATTCATCAGCATCTCATCGGAGGTCTGCAAATACAATCACTTGATGATGAATGGATCGATGTCGAGCCCTCGGGGCACTGCTCGTTTATCGTCATGGCTGGCGATGCAATCATGGGTTGGAGCAACGAGAGGATAAGGGCTTGCAGGCACCGAGTGATAATGAGAGGCAACGAAAGAAGATACTCAGTTGGGTTATTTTCGTTCAAAAGTGGAACAATTGGTGTGCCTGAGGAACTTGTGGACGACGCCAACCCACTGCGATACAAGCCCTTTGATCACTTCCAGTTCCTTAGCTATGACAAAGCCAATGCTTCCCATAAAACTGTCTCTCGTATTAAAGATTATTGCGGCCTTTGA

Protein sequence

MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPAGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVPEKDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKANASHKTVSRIKDYCGL

Homology

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BLAST of Lag0002949 vs. NCBI nr
Match: XP_022145687.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Momordica charantia])

HSP 1 Score: 525.8 bits (1353), Expect = 2.6e-145
Identity = 250/319 (78.37%), Postives = 285/319 (89.34%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MG+QAK+KIPTI+LS+EKLK  G+EEW L  KE+RVALEEFGCFLVVY++VSLELHNSIF
Sbjct: 1   MGTQAKSKIPTIELSEEKLK-VGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPA 120
           EATKELF LP+E+RIQNTSEKPYHGYFGRYSFLPLYESMA+DNPT LQA Q F NLMWPA
Sbjct: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPA 120

Query: 121 GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPK-LSDSNIESTNHLLRCFKYRVPEKD 180
           GN  FCETV +FST+VAKL+E VTRMV ESYGV K + +S+IEST HLLRCFKYRVP++D
Sbjct: 121 GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQD 180

Query: 181 ETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDD----EWIDVEPSGHCSFIVMAGDAIMG 240
           ETDLGLHSHTDLTF SI+HQHLIGGLQ++SLD+    EWID++P G  SFIVMAGDA+M 
Sbjct: 181 ETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDALMA 240

Query: 241 WSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSY 300
           WSN+RIR CRHRV+MRG+E RYS+GLFSFKSGTI VPEELV+D +PL+YKPFDHF+FL Y
Sbjct: 241 WSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRY 300

Query: 301 DKANASHKTVSRIKDYCGL 315
           DKANASHKT SRIKDYCG+
Sbjct: 301 DKANASHKTCSRIKDYCGI 318

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BLAST of Lag0002949 vs. NCBI nr
Match: XP_038898684.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-144
Identity = 246/317 (77.60%), Postives = 282/317 (88.96%), Query Frame = 0


Query: 1   MGSQAKAKI-PTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSI 60
           + S+AKAK+ PTIDLS  KL+A GTEEW L+CKEMR+ LEEFGCF VVYD VSLELHNSI
Sbjct: 2   VNSEAKAKVLPTIDLS--KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSI 61

Query: 61  FEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWP 120
           FEATKELF LPLE+RI+NTSEKPYHGYFG YS LPLYESM IDNP +LQA Q+FANLMWP
Sbjct: 62  FEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWP 121

Query: 121 A-GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPKL-SDSNIESTNHLLRCFKYRVPE 180
              NNHFC TV+ FST+VA+L++MVT+MV ESYG+ KL ++ N+ES N+LLRCFKYR PE
Sbjct: 122 TKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPE 181

Query: 181 KDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWS 240
           KDETD+GLHSHTDLTFFSIIHQHLI GLQ+QSLDD+WID++PSGHC FIVMAGDA+M WS
Sbjct: 182 KDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWS 241

Query: 241 NERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDK 300
           NERIR+CRHRVIMRG E RYS+G+FSFK+GTI VPEELVDDANPLRYKPF+H+ FL+YDK
Sbjct: 242 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDK 301

Query: 301 ANASHKTVSRIKDYCGL 315
           A ASH+ VSRIKDYCG+
Sbjct: 302 AKASHQNVSRIKDYCGI 316

[Close]

BLAST of Lag0002949 vs. NCBI nr
Match: XP_022992484.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 499.6 bits (1285), Expect = 2.0e-137
Identity = 233/316 (73.73%), Postives = 272/316 (86.08%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MGS+ +A++P IDLSK +   AGTE W  +CK MRV LEEFGCF VV++EVSLELHNS F
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPA 120
            A  ELF LP+E+RI+NTSEK YHGYFGRYSFLPLYESMAI+NPT L AAQ+FANLMWPA
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 121 -GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPK-LSDSNIESTNHLLRCFKYRVPEK 180
            GNNHF ET+Q FST++AKLD+++TRMV ESYGV K L D+NI+ST +LLR FKYRVPE+
Sbjct: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180

Query: 181 DETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSN 240
            ETD+GLH+HTDLTFFSIIHQHLIGGLQIQSLDD+WID+EPSGHCSF+VMAGDA+M WSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240

Query: 241 ERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKA 300
           +RIR+CRHRV M+  E RYS G+FSFK+GTI VPEELVDDANPLRYKPF+HF FL+YDKA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300

Query: 301 NASHKTVSRIKDYCGL 315
             S K + RI+DYC +
Sbjct: 301 KGSKKFLYRIRDYCSI 316

[Close]

BLAST of Lag0002949 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 499.2 bits (1284), Expect = 2.6e-137
Identity = 238/315 (75.56%), Postives = 274/315 (86.98%), Query Frame = 0


Query: 3   SQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEA 62
           +++K  IPTIDLSK ++   GT+EW L+CKEMR  LEEFGCF VVYD+VSLELHNSI EA
Sbjct: 5   AKSKVVIPTIDLSKLEV---GTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEA 64

Query: 63  TKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPA-G 122
           TKELF LP+E+R++NTSEKPYHGYFG YSFLPLYESMAIDNP  L++ Q+FANLMWP   
Sbjct: 65  TKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRD 124

Query: 123 NNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNI-ESTNHLLRCFKYRVPEKDE 182
           NNHFC TV+ FST+VAKL++MVT+MV ESYGV K+ +  I ESTN+LLR FKYRV EKDE
Sbjct: 125 NNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDE 184

Query: 183 TDLGLHSHTDLTFFSIIHQHLIGGLQIQSL-DDEWIDVEPSGHCSFIVMAGDAIMGWSNE 242
           TD+GLHSHTDLTFFSIIHQHLI GLQIQSL DD+W+DV PS HCSFIVMAGDA+M WSNE
Sbjct: 185 TDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNE 244

Query: 243 RIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKAN 302
           RIR+CRHRVIMRG E RYS+G+FSFK+GTI VP+  VDDANPLRYKPF+H+ FL+YDKAN
Sbjct: 245 RIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKAN 304

Query: 303 ASHKTVSRIKDYCGL 315
           ASH T+SRIKDYCGL
Sbjct: 305 ASHNTISRIKDYCGL 316

[Close]

BLAST of Lag0002949 vs. NCBI nr
Match: XP_022953943.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 489.6 bits (1259), Expect = 2.1e-134
Identity = 231/316 (73.10%), Postives = 268/316 (84.81%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MGS+ +A +P IDLSK +   AGT+ W  +CK MRV LEEFGCF VV++EVSLELHNS F
Sbjct: 1   MGSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWP- 120
            A  ELF LP+E+RI+NTSEK YHGYFGRYSFLPLYESMAIDNPT L AAQ+FANLMWP 
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 121 AGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPK-LSDSNIESTNHLLRCFKYRVPEK 180
            GNN F ET+Q FST+VAKLD++VTRMV ESYGV K L D+NI+ST +LLR FKYRVPE+
Sbjct: 121 QGNNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQ 180

Query: 181 DETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSN 240
            ETD+GLH+HTDLTFFSIIHQHLIGGLQIQSLDD+WID+EPS HCSFIVMAGDA+M WSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240

Query: 241 ERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKA 300
           +RIR+CRHRV M+  E RYS G+FSFK+GTI VPEELVDDANPLRYKPF+H+ FL++DKA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKA 300

Query: 301 NASHKTVSRIKDYCGL 315
             S K + RI+DYC +
Sbjct: 301 KGSKKFLYRIRDYCSI 316

[Close]

BLAST of Lag0002949 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.0e-60
Identity = 124/301 (41.20%), Postives = 183/301 (60.80%), Query Frame = 0


Query: 8   KIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEATKELF 67
           ++P ID S + LK  G+ +W     ++  ALE++GCF   +D++S+EL+ S+FEA ++LF
Sbjct: 13  QLPVIDFSDQNLK-PGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLF 72

Query: 68  DLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWP-AGNNHFC 127
           +LP+ ++ +N S KP+HGY        LYES+ ID+   L+    F   +WP  GN    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 128 ETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVPEKD-----ET 187
           ET+  FS  + +LD MV RM+ ES+G+    D ++ ST +L R  KY  P  D     ET
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 188 DLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSNERI 247
            LGL SHTD    +I+HQ+ + GL++++ DD+WI V+PS   S +VM GD++    N R+
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGRL 252

Query: 248 RACRHRVIMRGNERRYSVGLFSF-KSGT-IGVPEELVDDANPLRYKPFDHFQFLSYDKAN 301
            +  HRVIM G + RYS GLFS  K+G  I  PEELVD  +P  +KPF++  FL + +  
Sbjct: 253 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 307

[Close]

BLAST of Lag0002949 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 7.7e-60
Identity = 123/302 (40.73%), Postives = 183/302 (60.60%), Query Frame = 0


Query: 8   KIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEATKELF 67
           ++P ID S + LK  G+ +W     ++  ALE++GCF   +D++S+EL+ S+FEA ++LF
Sbjct: 13  QLPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 68  DLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWP-AGNNHFC 127
           +LP+ ++ +N S KP+HGY        LYES+ I++   L+    F   +WP  GN    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 128 ETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVPEKD------E 187
           ET+  FS  + +LD MV RM+ ES+G+    D ++ ST +L R  KY  P  D      E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 188 TDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSNER 247
           T LGL SHTD    +I+HQ+ + GL++++ DD+WI V+PS   S +VM GD++    N R
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGR 252

Query: 248 IRACRHRVIMRGNERRYSVGLFSF-KSGT-IGVPEELVDDANPLRYKPFDHFQFLSYDKA 301
           + +  HRVIM G + RYS GLFS  K+G  I  PEELVD  +P  +KPF++  FL + + 
Sbjct: 253 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 308

[Close]

BLAST of Lag0002949 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-59
Identity = 124/311 (39.87%), Postives = 175/311 (56.27%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MGS A  ++P IDLS + LK  GT  W     ++R ALEE+GCF  V D VS EL  ++ 
Sbjct: 1   MGSTAPLRLPVIDLSMKNLK-PGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVC 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPA 120
               EL +LPLE+++ N ++  Y G+    S   L E M +   TDL+  + F NLMWP 
Sbjct: 61  NKGHELLNLPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWPE 120

Query: 121 GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVPEKDE 180
           GN  FCETV ++   +A++D ++  MVFES+G+ K  DS  ESTN+LL   +Y+ P KD 
Sbjct: 121 GNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDG 180

Query: 181 TDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSNER 240
               L  H D +  +I++Q+ + GL+ ++ D EWI      H   IV+ GD  M WSN R
Sbjct: 181 RSPALSLHKDKSILTIVNQNDVKGLEFETKDGEWILPTADNH---IVLLGDCFMAWSNGR 240

Query: 241 IRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKANA 300
           + +  HRV +  N+ R S   FSF    I  P ELVD+ +PL + PF+  + L+Y     
Sbjct: 241 LHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKE 300

Query: 301 SHKTVSRIKDY 312
             K V  +K Y
Sbjct: 301 GAKAVCDLKQY 304

[Close]

BLAST of Lag0002949 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.7e-59
Identity = 123/300 (41.00%), Postives = 183/300 (61.00%), Query Frame = 0


Query: 8   KIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEATKELF 67
           ++P ID S + LK  G+ +W     ++  ALE++GCF   +D++S+EL+ S+FEA ++LF
Sbjct: 6   QLPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 65

Query: 68  DLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWP-AGNNHFC 127
           +LP+ ++ +N S K +HGY        LYES+ I++   L+    F   +WP  GN    
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 128 ETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVPEKD----ETD 187
           ET+  FS  + +LD MV RM+ ES+G+ K  D ++ ST +L R  KY  P  D    ET 
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 188 LGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSNERIR 247
           LGL SHTD    +I+HQ+ + GL++++ DD+WI V+PS   S +VM GD++    N R+ 
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGRLH 245

Query: 248 ACRHRVIMRGNERRYSVGLFSF-KSGT-IGVPEELVDDANPLRYKPFDHFQFLSYDKANA 301
           +  HRVIM G + RYS GLFS  K+G  I  PEELVD  +P  +KPF++  FL + +  A
Sbjct: 246 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 299

[Close]

BLAST of Lag0002949 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 8.6e-43
Identity = 121/435 (27.82%), Postives = 191/435 (43.91%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MGS    ++P I+L+ + L+  G+ +WA    ++R ALE+FGCF   YD+VSLEL  SI 
Sbjct: 1   MGS-CSLQLPLINLADKTLE-PGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIM 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWP- 120
           +  +ELF LP+E++ +N   KPY GY    +   L ES+ I N   L+    F   +WP 
Sbjct: 61  KTMEELFALPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPD 120

Query: 121 -AGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVP-- 180
             GN +  +T+Q F+  + ++D MV RMV ES+G+ K  D +++ST + +R  KY  P  
Sbjct: 121 GDGNENISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GDANTTVDDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVND 240

Query: 241 ------------------------------------------------------EKDETD 300
                                                                 + +E  
Sbjct: 241 NLNVDAEANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKK 300

Query: 301 LGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSNERIR 315
           LGL  HTD   F+++ QH I GL++++ D++WI V+PS + +FIV+AGD++    N RIR
Sbjct: 301 LGLPCHTDKNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPN-TFIVIAGDSLCALMNGRIR 360

[Close]

BLAST of Lag0002949 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 250/319 (78.37%), Postives = 285/319 (89.34%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MG+QAK+KIPTI+LS+EKLK  G+EEW L  KE+RVALEEFGCFLVVY++VSLELHNSIF
Sbjct: 1   MGTQAKSKIPTIELSEEKLK-VGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPA 120
           EATKELF LP+E+RIQNTSEKPYHGYFGRYSFLPLYESMA+DNPT LQA Q F NLMWPA
Sbjct: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPA 120

Query: 121 GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPK-LSDSNIESTNHLLRCFKYRVPEKD 180
           GN  FCETV +FST+VAKL+E VTRMV ESYGV K + +S+IEST HLLRCFKYRVP++D
Sbjct: 121 GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQD 180

Query: 181 ETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDD----EWIDVEPSGHCSFIVMAGDAIMG 240
           ETDLGLHSHTDLTF SI+HQHLIGGLQ++SLD+    EWID++P G  SFIVMAGDA+M 
Sbjct: 181 ETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDALMA 240

Query: 241 WSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSY 300
           WSN+RIR CRHRV+MRG+E RYS+GLFSFKSGTI VPEELV+D +PL+YKPFDHF+FL Y
Sbjct: 241 WSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRY 300

Query: 301 DKANASHKTVSRIKDYCGL 315
           DKANASHKT SRIKDYCG+
Sbjct: 301 DKANASHKTCSRIKDYCGI 318

[Close]

BLAST of Lag0002949 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 9.6e-138
Identity = 233/316 (73.73%), Postives = 272/316 (86.08%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MGS+ +A++P IDLSK +   AGTE W  +CK MRV LEEFGCF VV++EVSLELHNS F
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPA 120
            A  ELF LP+E+RI+NTSEK YHGYFGRYSFLPLYESMAI+NPT L AAQ+FANLMWPA
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 121 -GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPK-LSDSNIESTNHLLRCFKYRVPEK 180
            GNNHF ET+Q FST++AKLD+++TRMV ESYGV K L D+NI+ST +LLR FKYRVPE+
Sbjct: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180

Query: 181 DETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSN 240
            ETD+GLH+HTDLTFFSIIHQHLIGGLQIQSLDD+WID+EPSGHCSF+VMAGDA+M WSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240

Query: 241 ERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKA 300
           +RIR+CRHRV M+  E RYS G+FSFK+GTI VPEELVDDANPLRYKPF+HF FL+YDKA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300

Query: 301 NASHKTVSRIKDYCGL 315
             S K + RI+DYC +
Sbjct: 301 KGSKKFLYRIRDYCSI 316

[Close]

BLAST of Lag0002949 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.3e-137
Identity = 238/315 (75.56%), Postives = 274/315 (86.98%), Query Frame = 0


Query: 3   SQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEA 62
           +++K  IPTIDLSK ++   GT+EW L+CKEMR  LEEFGCF VVYD+VSLELHNSI EA
Sbjct: 5   AKSKVVIPTIDLSKLEV---GTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEA 64

Query: 63  TKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPA-G 122
           TKELF LP+E+R++NTSEKPYHGYFG YSFLPLYESMAIDNP  L++ Q+FANLMWP   
Sbjct: 65  TKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRD 124

Query: 123 NNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNI-ESTNHLLRCFKYRVPEKDE 182
           NNHFC TV+ FST+VAKL++MVT+MV ESYGV K+ +  I ESTN+LLR FKYRV EKDE
Sbjct: 125 NNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDE 184

Query: 183 TDLGLHSHTDLTFFSIIHQHLIGGLQIQSL-DDEWIDVEPSGHCSFIVMAGDAIMGWSNE 242
           TD+GLHSHTDLTFFSIIHQHLI GLQIQSL DD+W+DV PS HCSFIVMAGDA+M WSNE
Sbjct: 185 TDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNE 244

Query: 243 RIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKAN 302
           RIR+CRHRVIMRG E RYS+G+FSFK+GTI VP+  VDDANPLRYKPF+H+ FL+YDKAN
Sbjct: 245 RIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKAN 304

Query: 303 ASHKTVSRIKDYCGL 315
           ASH T+SRIKDYCGL
Sbjct: 305 ASHNTISRIKDYCGL 316

[Close]

BLAST of Lag0002949 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 9.9e-135
Identity = 231/316 (73.10%), Postives = 268/316 (84.81%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MGS+ +A +P IDLSK +   AGT+ W  +CK MRV LEEFGCF VV++EVSLELHNS F
Sbjct: 1   MGSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWP- 120
            A  ELF LP+E+RI+NTSEK YHGYFGRYSFLPLYESMAIDNPT L AAQ+FANLMWP 
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 121 AGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPK-LSDSNIESTNHLLRCFKYRVPEK 180
            GNN F ET+Q FST+VAKLD++VTRMV ESYGV K L D+NI+ST +LLR FKYRVPE+
Sbjct: 121 QGNNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQ 180

Query: 181 DETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSN 240
            ETD+GLH+HTDLTFFSIIHQHLIGGLQIQSLDD+WID+EPS HCSFIVMAGDA+M WSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240

Query: 241 ERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKA 300
           +RIR+CRHRV M+  E RYS G+FSFK+GTI VPEELVDDANPLRYKPF+H+ FL++DKA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKA 300

Query: 301 NASHKTVSRIKDYCGL 315
             S K + RI+DYC +
Sbjct: 301 KGSKKFLYRIRDYCSI 316

[Close]

BLAST of Lag0002949 vs. ExPASy TrEMBL
Match: A0A6P5SM26 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Prunus avium OX=42229 GN=LOC110760070 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 3.8e-110
Identity = 185/314 (58.92%), Postives = 245/314 (78.03%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MGS+ +AK+P +DLS E LK  GTE W L+ K+++ ALEE+GCF  V+ +V L+LHNSIF
Sbjct: 1   MGSETQAKLPVVDLSNENLK-PGTEAWVLASKQVKYALEEYGCFEAVFHKVPLQLHNSIF 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPA 120
              ++LFDLP+E++ Q TSE+PYH YFG+YSFLPLYES+ +DNPT ++ AQ F  +MWPA
Sbjct: 61  SMVEDLFDLPIETKRQQTSERPYHSYFGQYSFLPLYESLGVDNPTTVEGAQGFTKIMWPA 120

Query: 121 GNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVPEKDE 180
           GN+HFCE+ Q+FS +VA+LD+MVTRMVF+ YGV +L DS++ ST +LLRCFKYR  +++E
Sbjct: 121 GNDHFCESAQSFSKLVAELDQMVTRMVFDIYGVERLYDSHMASTTYLLRCFKYREQKENE 180

Query: 181 TDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSNER 240
           T++GL  H D TF SI+HQ  + GLQI+S D +WID EPS   SF+VMAGDA M WSN+R
Sbjct: 181 TNVGLRPHKDKTFTSILHQSQVHGLQIRSKDGQWIDAEPS-PSSFLVMAGDAFMAWSNDR 240

Query: 241 IRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKANA 300
           +R+C H+VIM+ N+ RYS+GLFSF SG + VPEEL+DD +PL YKPFDHF FL +D+   
Sbjct: 241 VRSCDHQVIMKENKTRYSLGLFSFNSGILEVPEELIDDQHPLMYKPFDHFGFLRFDQTPE 300

Query: 301 SHKTVSRIKDYCGL 315
           + K+   IK YCG+
Sbjct: 301 AKKSECPIKAYCGV 312

[Close]

BLAST of Lag0002949 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 311.2 bits (796), Expect = 9.3e-85
Identity = 142/307 (46.25%), Postives = 204/307 (66.45%), Query Frame = 0


Query: 8   KIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIFEATKELF 67
           K+P +DL+ ++     T  W    +E   ALEE+GCFL VYD V+ +L +SIF A +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  DLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPAGNNHFCE 127
           DLP E++ +N +EKPYHGY G+   +PL+E + +D  T+ + AQ F +LMWP GN+ FC 
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRFCN 128

Query: 128 TVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVPEKDETDLGLHS 187
           TV  FS  VA+LD +V RM+FE+YGV K  +S++ S  +LL+  KY  P +  +      
Sbjct: 129 TVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMPAFPQ 188

Query: 188 HTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSNERIRACRHR 247
           HTD TF SI+HQ+ + GL+++S D EWI ++     S++VMAGD  MGWSN+RIR+C HR
Sbjct: 189 HTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPK-SYVVMAGDISMGWSNDRIRSCEHR 248

Query: 248 VIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKANASHKTVSR 307
           V M G++ RY++GLFSF +  + +PEELVDD +PL YKPFD+   +++       +  S 
Sbjct: 249 VTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANST 308

Query: 308 IKDYCGL 315
           +K YCG+
Sbjct: 309 LKAYCGI 314

[Close]

BLAST of Lag0002949 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 300.1 bits (767), Expect = 2.1e-81
Identity = 139/307 (45.28%), Postives = 209/307 (68.08%), Query Frame = 0


Query: 8   KIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLV-VYDEVSLELHNSIFEATKEL 67
           KIPT+D S+E LK  GT+ W  + + +R ALEE+GCF++ + D+  L+L + +F +  +L
Sbjct: 4   KIPTLDFSREDLK-PGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDL 63

Query: 68  FDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLMWPAGNNHFC 127
           FDLP +++++N  +KP +GY G+   LPL+ES+ IDN T L+A ++F  LMWP GN HF 
Sbjct: 64  FDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNEHFS 123

Query: 128 ETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVPEKDETDLGLH 187
           E +  ++   A+LD+MVTRMVF+SY V K  D  IEST +LLR  K R P  +   LG  
Sbjct: 124 ECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGFV 183

Query: 188 SHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWSNERIRACRH 247
           +HTD +F +I+HQ  + GL++++ + E I++  S    F+V+AGDA+M WSN+R+ + RH
Sbjct: 184 THTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPRH 243

Query: 248 RVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDKANASHKTVS 307
           +V++ G   RYS+G+F+F +GT+ VPEEL+D  +PL YKPFDH   L + + +  +K+  
Sbjct: 244 QVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSEC 303

Query: 308 RIKDYCG 314
            IK YCG
Sbjct: 304 PIKAYCG 309

[Close]

BLAST of Lag0002949 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 7.9e-76
Identity = 135/319 (42.32%), Postives = 208/319 (65.20%), Query Frame = 0


Query: 1   MGSQA---KAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHN 60
           MGS+      ++P ID S + LK  G  EW L+  +++ AL+++G F   +D +  EL  
Sbjct: 1   MGSETPLLPLRLPVIDFSNKNLK-PGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK 60

Query: 61  SIFEATKELFDLPLESRIQNTSEKPYHGYFGRYSFLPLYESMAIDNPTDLQAAQAFANLM 120
           S+F A +ELFDLPL+++++N S+KP+HGY G+Y  +PLYESM ID+    +   AF   +
Sbjct: 61  SVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKL 120

Query: 121 WPAGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVPE 180
           WP GN  F  T+Q+FS  +++LD  + RM+ ES+G+ K  D ++ STN+LLR  KY+ P+
Sbjct: 121 WPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPD 180

Query: 181 KDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWS 240
            +ET +GL++HTD    +I++Q+ + GL++Q+ D  WI V+P+   SF VM GD++    
Sbjct: 181 TEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPT-QDSFTVMIGDSLYALL 240

Query: 241 NERIRACRHRVIMRGNERRYSVGLFSF-KSG-TIGVPEELVDDANPLRYKPFDHFQFLSY 300
           N R+ +  HRV+M G E RYS+GLFS  K+G  +  P+ELVD+ +P  +KPFDH +FL +
Sbjct: 241 NGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQF 300

Query: 301 DKANASHKTVSRIKDYCGL 315
               A  ++ S +K YCG+
Sbjct: 301 YYTEAGQRSQSALKTYCGI 317

[Close]

BLAST of Lag0002949 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 7.9e-68
Identity = 138/321 (42.99%), Postives = 188/321 (58.57%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           MG     ++P I+LS + LK   TE W+ +   +R A+E  G F+  Y+     LH SI 
Sbjct: 1   MGGNETRELPIINLSDKNLK-QNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSIL 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGRYS-FLPLYESMAIDNPTDLQAAQAFANLMWP 120
           EA KEL DLP+E +++N + K  HGY    S   P++E + ID   D+Q  + F+ LMWP
Sbjct: 61  EAAKELLDLPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWP 120

Query: 121 ---AGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGVPKLSDSNIESTNHLLRCFKYRVP 180
                N+ FCETV  ++ M A+L+++V RM+FESY V K ++  I  T +LLR  KYR  
Sbjct: 121 DDHDDNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRL 180

Query: 181 EKDETDLGLHSHTDLTFFSIIHQHLIGGLQIQS-LDDEWIDVEPSGHCSFIVMAGDAIMG 240
              E +    SHTD +F SI+HQ+ I GL ++S  +D W    PS    F+V+AGDAIM 
Sbjct: 181 PNGEPNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPS-PTRFVVIAGDAIMA 240

Query: 241 WSNERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSY 300
           WSN+RI+AC H+V M   E RYS+G FSF+ G I  PEE+VD  +PL Y PF H   L Y
Sbjct: 241 WSNDRIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEY 300

Query: 301 DKANASHKTVSR--IKDYCGL 315
            +    H    R   K YCG+
Sbjct: 301 YETLEVHLKAHRTMTKAYCGI 319

[Close]

BLAST of Lag0002949 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 5.1e-67
Identity = 126/320 (39.38%), Postives = 197/320 (61.56%), Query Frame = 0


Query: 1   MGSQAKAKIPTIDLSKEKLKAAGTEEWALSCKEMRVALEEFGCFLVVYDEVSLELHNSIF 60
           M S  +  +P +D S +KL   GT  W  +  ++R A+E  G F+  +  VS +L +++ 
Sbjct: 1   MDSHEEQCLPILDFSSDKL-VRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLL 60

Query: 61  EATKELFDLPLESRIQNTSEKPYHGYFGR-YSFLPLYESMAIDNPTDLQAAQAFANLMWP 120
              KE++ LP + +I+N + K  HGY         ++ES+ ID  T+LQA + F+ L+WP
Sbjct: 61  AGMKEMYYLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP 120

Query: 121 AGNNHFCETVQNFSTMVAKLDEMVTRMVFESYGV--PKLSDSNIESTNHLLRCFKYRVPE 180
            GN+ FC+T   ++  +A+LD+ V RM++ESYG+   K S S+ EST +LLR   YR  +
Sbjct: 121 QGNDPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQ 180

Query: 181 KDETDLGLHSHTDLTFFSIIHQHLIGGLQIQSLDDEWIDVEPSGHCSFIVMAGDAIMGWS 240
             E + G  SHTD +F SI+HQ+ +GGLQ++++  +W+   PS    F+V++G  +  WS
Sbjct: 181 NGEANTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPS-PTRFVVLSGMGLTAWS 240

Query: 241 NERIRACRHRVIMRGNERRYSVGLFSFKSGTIGVPEELVDDANPLRYKPFDHFQFLSYDK 300
           N+RI+AC H+V+M  +E RYS+G FSF  GTI  PEELVDD +PLRY PF+H   L + +
Sbjct: 241 NDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYE 300

Query: 301 A---NASHKTVSRIKDYCGL 315
           +   +    +   ++ YCG+
Sbjct: 301 SYINSLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:

BLAST of Lag0002949 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145687.1	2.6e-145	78.37	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Momordica charantia]	[more]
XP_038898684.1	1.3e-144	77.60	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
XP_022992484.1	2.0e-137	73.73	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima]	[more]
XP_008461057.1	2.6e-137	75.56	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi...	[more]
XP_022953943.1	2.1e-134	73.10	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]

BLAST of Lag0002949 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	2.0e-60	41.20	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	7.7e-60	40.73	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
A0A0N9HQ36	1.3e-59	39.87	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q944Z5	1.7e-59	41.00	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
Q945B5	8.6e-43	27.82	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Lag0002949 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXE5	1.3e-145	78.37	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367...	[more]
A0A6J1JXN6	9.6e-138	73.73	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A1S3CDV2	1.3e-137	75.56	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX...	[more]
A0A6J1GPR2	9.9e-135	73.10	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6P5SM26	3.8e-110	58.92	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Prunus avium OX=42229 GN=L...	[more]

BLAST of Lag0002949 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52800.1	9.3e-85	46.25	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	2.1e-81	45.28	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	7.9e-76	42.32	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	7.9e-68	42.99	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	5.1e-67	39.38	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 166..264 e-value: 1.0E-15 score: 58.0
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 9..88 e-value: 2.1E-11 score: 44.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..302 e-value: 4.6E-58 score: 199.0
None	No IPR available	PANTHER	PTHR47990:SF169	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE	coord: 5..313
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 5..313
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 7..294
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 163..267 score: 10.353865

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002949.1	Lag0002949.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding