Lag0002921 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002921
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionMAPK kinase substrate protein At1g80180-like
Locationchr4: 46724261 .. 46724611 (-)
RNA-Seq ExpressionLag0002921
SyntenyLag0002921
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGTTTGCAGAGATCTGTGGTGTCCTTCCGGCGGCAGGGATCTTCCGGTCTTGTCTGGGACGATAAGTTCATCTCCGAGGAGCTCAACAAGCCGCCGCAAAACGACGGAGAATCCAGCGCGCCGGAGGAGGTGACGACGGGAAGATATATTCCGCCGATCAAGACGATTTCCCGGAGCCGATCCAACCGCGCCGGAGGACGGGGGTTCCGGAGTAAAGAGACGGCAGCGCCGGCGGTGGAGCCGCCGTCTCCGAGAGTCTCTGCTTGTGGGCTCTGCAGCGCTTTCGGGAAATCCGGCGCCAAGAAACAGAGGGCGACGGCGACCGGAAAGCGCCGATCACGGTAG

mRNA sequence

ATGGCTGGTTTGCAGAGATCTGTGGTGTCCTTCCGGCGGCAGGGATCTTCCGGTCTTGTCTGGGACGATAAGTTCATCTCCGAGGAGCTCAACAAGCCGCCGCAAAACGACGGAGAATCCAGCGCGCCGGAGGAGGTGACGACGGGAAGATATATTCCGCCGATCAAGACGATTTCCCGGAGCCGATCCAACCGCGCCGGAGGACGGGGGTTCCGGAGTAAAGAGACGGCAGCGCCGGCGGTGGAGCCGCCGTCTCCGAGAGTCTCTGCTTGTGGGCTCTGCAGCGCTTTCGGGAAATCCGGCGCCAAGAAACAGAGGGCGACGGCGACCGGAAAGCGCCGATCACGGTAG

Coding sequence (CDS)

ATGGCTGGTTTGCAGAGATCTGTGGTGTCCTTCCGGCGGCAGGGATCTTCCGGTCTTGTCTGGGACGATAAGTTCATCTCCGAGGAGCTCAACAAGCCGCCGCAAAACGACGGAGAATCCAGCGCGCCGGAGGAGGTGACGACGGGAAGATATATTCCGCCGATCAAGACGATTTCCCGGAGCCGATCCAACCGCGCCGGAGGACGGGGGTTCCGGAGTAAAGAGACGGCAGCGCCGGCGGTGGAGCCGCCGTCTCCGAGAGTCTCTGCTTGTGGGCTCTGCAGCGCTTTCGGGAAATCCGGCGCCAAGAAACAGAGGGCGACGGCGACCGGAAAGCGCCGATCACGGTAG

Protein sequence

MAGLQRSVVSFRRQGSSGLVWDDKFISEELNKPPQNDGESSAPEEVTTGRYIPPIKTISRSRSNRAGGRGFRSKETAAPAVEPPSPRVSACGLCSAFGKSGAKKQRATATGKRRSR
Homology
BLAST of Lag0002921 vs. NCBI nr
Match: XP_022991595.1 (MAPK kinase substrate protein At1g80180-like [Cucurbita maxima])

HSP 1 Score: 192.2 bits (487), Expect = 2.5e-45
Identity = 100/116 (86.21%), Postives = 104/116 (89.66%), Query Frame = 0

Query: 1   MAGLQRSVVSFRRQGSSGLVWDDKFISEELNKPPQNDGESSAPEEVTTGRYIPPIKTISR 60
           MAGLQRSVVSFRRQGSSG+VWDDKFISEELNK   +DGESSA  EV TGR IPPIKTI R
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWDDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60

Query: 61  SRSNRAGGRGFRSKETAAPAVEPPSPRVSACGLCSAFGKSGAKKQRATATGKRRSR 117
           +RSNR GGRGFRSK+ AAPAVEPPSPRVSACGLCSAFGKSGAKKQRA A GKRRSR
Sbjct: 61  ARSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAGKRRSR 116

BLAST of Lag0002921 vs. NCBI nr
Match: XP_023549291.1 (MAPK kinase substrate protein At1g80180-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 189.5 bits (480), Expect = 1.6e-44
Identity = 101/117 (86.32%), Postives = 104/117 (88.89%), Query Frame = 0

Query: 1   MAGLQRSVVSFRRQGSSGLVWDDKFISEELNKPPQNDGESSAPEEVTTGRYIPPIKTISR 60
           MAGLQRSVVSFRRQGSSG+VWDDKFISEELNK   NDGESSA  EV TGR IPPIKTI R
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWDDKFISEELNKAAPNDGESSATPEVKTGRNIPPIKTIDR 60

Query: 61  SRSNRAGGRGFRSKETAAPAVEPPSPRVSACGLCSAFGKSGAKKQR-ATATGKRRSR 117
           +RSNR GGRGFRSK+ AAPAVEPPSPRVSACGLCSAFGKSGAKKQR A A GKRRSR
Sbjct: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAGKRRSR 117

BLAST of Lag0002921 vs. NCBI nr
Match: KAG6575586.1 (MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 186.0 bits (471), Expect = 1.8e-43
Identity = 99/117 (84.62%), Postives = 104/117 (88.89%), Query Frame = 0

Query: 1   MAGLQRSVVSFRRQGSSGLVWDDKFISEELNKPPQNDGESSAPEEVTTGRYIPPIKTISR 60
           MAGLQRSVVSFRRQGSSG+VW+DKFISEELNK   +DGESSA  EV TGR IPPIKTI R
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60

Query: 61  SRSNRAGGRGFRSKETAAPAVEPPSPRVSACGLCSAFGKSGAKKQR-ATATGKRRSR 117
           +RSNR GGRGFRSK+ AAPAVEPPSPRVSACGLCSAFGKSGAKKQR A A GKRRSR
Sbjct: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAGKRRSR 117

BLAST of Lag0002921 vs. NCBI nr
Match: KAG7014127.1 (MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 185.7 bits (470), Expect = 2.3e-43
Identity = 99/118 (83.90%), Postives = 104/118 (88.14%), Query Frame = 0

Query: 1   MAGLQRSVVSFRRQGSSGLVWDDKFISEELNKPPQNDGESSAPEEVTTGRYIPPIKTISR 60
           MAGLQRSVVSFRRQGSSG+VW+DKFISEELNK   +DGESSA  EV TGR IPPIKTI R
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60

Query: 61  SRSNRAGGRGFRSKETAAPAVEPPSPRVSACGLCSAFGKSGAKKQR--ATATGKRRSR 117
           +RSNR GGRGFRSK+ AAPAVEPPSPRVSACGLCSAFGKSGAKKQR  A A GKRRSR
Sbjct: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 118

BLAST of Lag0002921 vs. NCBI nr
Match: XP_023000641.1 (MAPK kinase substrate protein At1g80180-like [Cucurbita maxima])

HSP 1 Score: 177.6 bits (449), Expect = 6.4e-41
Identity = 93/117 (79.49%), Postives = 100/117 (85.47%), Query Frame = 0

Query: 1   MAGLQRSVVSFRRQGSSGLVWDDKFISEELNKPPQNDGESS-APEEVTTGRYIPPIKTIS 60
           MAGLQRSVVSFRRQGSSGL+WDDKFISEEL K PQND ESS A EE+ TGR IPPIKT+ 
Sbjct: 1   MAGLQRSVVSFRRQGSSGLIWDDKFISEELIKAPQNDAESSTAAEELNTGRNIPPIKTVD 60

Query: 61  RSRSNRAGGRGFRSKETAAPAVEPPSPRVSACGLCSAFGKSGAKKQRATATGKRRSR 117
           R+RSNR   RGFRSK+TAA AVEPPSPR+SACG+CSAF  SG KKQR TA GKRRSR
Sbjct: 61  RTRSNRGEARGFRSKDTAAAAVEPPSPRISACGICSAFRNSGGKKQRTTAAGKRRSR 117

BLAST of Lag0002921 vs. ExPASy Swiss-Prot
Match: Q9SSC1 (MAPK kinase substrate protein At1g80180 OS=Arabidopsis thaliana OX=3702 GN=At1g80180 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.3e-17
Identity = 66/139 (47.48%), Postives = 83/139 (59.71%), Query Frame = 0

Query: 1   MAGLQRSVVSFRRQGSSGLVWDDKFISEELNKPPQND--GE-----------SSAPEEVT 60
           MAGLQRS +SFRRQGSSG+VWDD+ I+ EL++   ND  GE           +S  ++ T
Sbjct: 1   MAGLQRSTISFRRQGSSGIVWDDRLIA-ELSQQAANDRKGETLQQDEQAKLITSEVQDQT 60

Query: 61  T----GRYIPPIKT---ISRSRSNRAGG-RGFRSKETAAPAVEPPSPRVSACGLCSAFGK 117
           T    G  + PI+T   + RSRSN  G  R  R+    +PAV+PPSPR+SA G CSAFGK
Sbjct: 61  TKPIAGEGLKPIRTDGGMERSRSNGGGAIRHHRNTGRVSPAVDPPSPRLSAFGCCSAFGK 120

BLAST of Lag0002921 vs. ExPASy Swiss-Prot
Match: Q9XI29 (Uncharacterized protein At1g15400 OS=Arabidopsis thaliana OX=3702 GN=At1g15400 PE=1 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 2.5e-16
Identity = 64/141 (45.39%), Postives = 79/141 (56.03%), Query Frame = 0

Query: 1   MAGLQRSVVSFRRQGSSGLVWDDKFISEELNKPPQND-------GESSAPEEVTTGRYIP 60
           M GLQRS +SFRRQGSSG+V+DD+ I+ ELNK   N+        E   P   ++ +  P
Sbjct: 1   MEGLQRSTISFRRQGSSGIVFDDRLIA-ELNKSGNNEQKDESQRDEQPKPMSESSEQVKP 60

Query: 61  --------PIKT-------ISRSRSNRAGG-RGFRSKETAAPAVEPPSPRVSACGLCSAF 117
                   PIKT       I RSRSN  G  R  R+    +PAV+PPSPR+S+CG CSAF
Sbjct: 61  IDEKDKLRPIKTGGGAPGGIERSRSNGGGAQRHHRTTGRVSPAVDPPSPRISSCGCCSAF 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022991595.12.5e-4586.21MAPK kinase substrate protein At1g80180-like [Cucurbita maxima][more]
XP_023549291.11.6e-4486.32MAPK kinase substrate protein At1g80180-like [Cucurbita pepo subsp. pepo][more]
KAG6575586.11.8e-4384.62MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7014127.12.3e-4383.90MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_023000641.16.4e-4179.49MAPK kinase substrate protein At1g80180-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SSC12.3e-1747.48MAPK kinase substrate protein At1g80180 OS=Arabidopsis thaliana OX=3702 GN=At1g8... [more]
Q9XI292.5e-1645.39Uncharacterized protein At1g15400 OS=Arabidopsis thaliana OX=3702 GN=At1g15400 P... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031421Protein of unknown function DUF4666PFAMPF15697DUF4666coord: 1..105
e-value: 2.8E-31
score: 108.5
IPR031421Protein of unknown function DUF4666PANTHERPTHR33730OS05G0542732 PROTEIN-RELATEDcoord: 1..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..87
NoneNo IPR availablePANTHERPTHR33730:SF4OS05G0542732 PROTEINcoord: 1..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002921.1Lag0002921.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity