Lag0002909 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002909
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Epidermal patterning factor-like protein
Location	chr4: 46629248 .. 46630025 (-)
RNA-Seq Expression	Lag0002909
Synteny	Lag0002909

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTTCTCATTCTCTCTTCCTTGTTCCTTTAAACTGGCTCTCACTGTTGCTTTGTTGTTCTTCTTCTCATTTTGTCCTTCTAAATCAGGTATTGAGAGTCTCTTTCTTAACCCCACTTGCTTGTTTTGGCATCGTGAAACTTGGGTTTTGTTCTTTTGCTTTCTCATTTGGTTACTTCCAAGACAGAGGCTAGCTAGTTTTTTAAGTTTGATATTTTAACTCTGTTTTCTTGTTTGAAACCTGTTGGGGCAGAGCTAGAGATTTTCAAAAAGTTGAAAACTTTGATGCTGTGACATTGAAAGTAAGAAGAAAACTTTGAGATTTTAAAAGGGTAATTGCTCATTTGCCTTTTTTTCTTTTAGTTCAAGTGATTTCGAATCAAATTTTTACGTTTTTGGAGATAAATTAAGAAGAAAAGTTGTAAACAGAGTAATTGCAACCTGCTTCCTTCTTCACCTGTTCTTGATAAAAATTTCATCTTTTTTCTTTTTGTTCAGTTGGGTTGTTTTCGTCGAGGAGAGGCAAAGGGGAAAAAGAAATGAAATATTTGGAAGAGGCTGAAACTGAAAGAGAAAGTTATGTGGAAGATGAAAAGAAAATGGTGATCGGTTCGAGGCCGCCGGGATGTGAGAACAAGTGCAAGAGTTGCAGGCCGTGCATGGCGGCGGTGGTGGTGCCGGTGCACAGGATGAAGGCTTCGACTAGAGAAGAAGAGGATAGCTATTATCTTCTCTCATGGAAATGCCGATGTGGGAATAAGATCTATCAGCCCTAG

mRNA sequence

ATGGCTTTCTCATTCTCTCTTCCTTGTTCCTTTAAACTGGCTCTCACTGTTGCTTTGTTGTTCTTCTTCTCATTTTGTCCTTCTAAATCAGTTGGGTTGTTTTCGTCGAGGAGAGGCAAAGGGGAAAAAGAAATGAAATATTTGGAAGAGGCTGAAACTGAAAGAGAAAGTTATGTGGAAGATGAAAAGAAAATGGTGATCGGTTCGAGGCCGCCGGGATGTGAGAACAAGTGCAAGAGTTGCAGGCCGTGCATGGCGGCGGTGGTGGTGCCGGTGCACAGGATGAAGGCTTCGACTAGAGAAGAAGAGGATAGCTATTATCTTCTCTCATGGAAATGCCGATGTGGGAATAAGATCTATCAGCCCTAG

Coding sequence (CDS)

ATGGCTTTCTCATTCTCTCTTCCTTGTTCCTTTAAACTGGCTCTCACTGTTGCTTTGTTGTTCTTCTTCTCATTTTGTCCTTCTAAATCAGTTGGGTTGTTTTCGTCGAGGAGAGGCAAAGGGGAAAAAGAAATGAAATATTTGGAAGAGGCTGAAACTGAAAGAGAAAGTTATGTGGAAGATGAAAAGAAAATGGTGATCGGTTCGAGGCCGCCGGGATGTGAGAACAAGTGCAAGAGTTGCAGGCCGTGCATGGCGGCGGTGGTGGTGCCGGTGCACAGGATGAAGGCTTCGACTAGAGAAGAAGAGGATAGCTATTATCTTCTCTCATGGAAATGCCGATGTGGGAATAAGATCTATCAGCCCTAG

Protein sequence

MAFSFSLPCSFKLALTVALLFFFSFCPSKSVGLFSSRRGKGEKEMKYLEEAETERESYVEDEKKMVIGSRPPGCENKCKSCRPCMAAVVVPVHRMKASTREEEDSYYLLSWKCRCGNKIYQP

Homology

[Close]

BLAST of Lag0002909 vs. NCBI nr
Match: KAG6593393.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 148.7 bits (374), Expect = 3.3e-32
Identity = 86/125 (68.80%), Postives = 91/125 (72.80%), Query Frame = 0


Query: 6   SLPCSFKLALTVALLFFF-SFCPSKSVGLFSSRRGKGEKEMKYLEEAETERESYVEDE-K 65
           SLP SFKL L +   FFF +F PSKS GLF  R G G KE   LE+   ERESYV DE K
Sbjct: 2   SLPSSFKLPLLLFFFFFFLTFLPSKS-GLFWPRSGGGGKETGNLEKG--ERESYVVDEQK 61

Query: 66  KMVIGSRPPGCENKCKSCRPCMAAVVVPVHRMK------ASTREEEDSYYLLSWKCRCGN 123
           KMVIGSRPPGCENKCKSCRPCMAA VVP HR K       S+  E+DSYYLLSWKCRCGN
Sbjct: 62  KMVIGSRPPGCENKCKSCRPCMAAAVVPAHRKKWFKAFVPSSGVEDDSYYLLSWKCRCGN 121

[Close]

BLAST of Lag0002909 vs. NCBI nr
Match: KAG7025740.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 147.9 bits (372), Expect = 5.7e-32
Identity = 86/127 (67.72%), Postives = 91/127 (71.65%), Query Frame = 0


Query: 6   SLPCSFKLALTVALLFFF---SFCPSKSVGLFSSRRGKGEKEMKYLEEAETERESYVEDE 65
           SLP SFKL L +   FFF   +F PSKS GLF  R G G KE   LE+   ERESYV DE
Sbjct: 2   SLPSSFKLPLLLFFFFFFFFLTFLPSKS-GLFWPRSGGGGKETGNLEKG--ERESYVVDE 61

Query: 66  -KKMVIGSRPPGCENKCKSCRPCMAAVVVPVHRMK------ASTREEEDSYYLLSWKCRC 123
            KKMVIGSRPPGCENKCKSCRPCMAA VVP HR K       S+  E+DSYYLLSWKCRC
Sbjct: 62  QKKMVIGSRPPGCENKCKSCRPCMAAAVVPAHRKKWFKAFVPSSGVEDDSYYLLSWKCRC 121

[Close]

BLAST of Lag0002909 vs. NCBI nr
Match: KAE8646441.1 (hypothetical protein Csa_015844 [Cucumis sativus])

HSP 1 Score: 143.3 bits (360), Expect = 1.4e-30
Identity = 81/129 (62.79%), Postives = 91/129 (70.54%), Query Frame = 0


Query: 1   MAFSFSLPCSFKLALTVALLFFFSFCPSKSVGLFSSRRGKGEKEMKYLEEAETERESYVE 60
           MA   SL  S KLALT+A   FFSF P  SVGL  SR G+ E     L +AE +    +E
Sbjct: 3   MALPISLSTSLKLALTLAA--FFSFLPPTSVGLVLSRSGRSE-----LGKAENDEMKNLE 62

Query: 61  DEKKMVIGSRPPGCENKCKSCRPCMAAVVVPVHRMK-------ASTREEEDSYYLLSWKC 120
           +  KMVIGSRPPGC+NKC +CRPCMAA VVPVHRMK       +S+REEEDSYYLLSWKC
Sbjct: 63  E--KMVIGSRPPGCQNKCMNCRPCMAAAVVPVHRMKGKAFQSFSSSREEEDSYYLLSWKC 122

Query: 121 RCGNKIYQP 123
           RCGNKIYQP
Sbjct: 123 RCGNKIYQP 122

[Close]

BLAST of Lag0002909 vs. NCBI nr
Match: XP_008461115.1 (PREDICTED: EPIDERMAL PATTERNING FACTOR-like protein 4 [Cucumis melo])

HSP 1 Score: 142.9 bits (359), Expect = 1.8e-30
Identity = 79/129 (61.24%), Postives = 92/129 (71.32%), Query Frame = 0


Query: 1   MAFSFSLPCSFKLALTVALLFFFSFCPSKSVGLFSSRRGKGEKEMKYLEEAETERESYVE 60
           MA   SL  S KLALT+A   FFSF P  SVGL  SR G+ E       +A+ +   ++E
Sbjct: 3   MALPISLSTSLKLALTLAA--FFSFLPPTSVGLVLSRSGRSE------GKADNDEMKFLE 62

Query: 61  DEKKMVIGSRPPGCENKCKSCRPCMAAVVVPVHRMK-------ASTREEEDSYYLLSWKC 120
           D+ KMVIGSRPPGC+NKC +CRPCMAA VVPVHRMK       +S+REEEDSYYLLSWKC
Sbjct: 63  DQ-KMVIGSRPPGCQNKCMNCRPCMAAAVVPVHRMKGKAFQSFSSSREEEDSYYLLSWKC 122

Query: 121 RCGNKIYQP 123
           +CGNKIYQP
Sbjct: 123 QCGNKIYQP 122

[Close]

BLAST of Lag0002909 vs. NCBI nr
Match: KAA0058756.1 (EPIDERMAL PATTERNING FACTOR-like protein 4 [Cucumis melo var. makuwa] >TYK10550.1 EPIDERMAL PATTERNING FACTOR-like protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 139.4 bits (350), Expect = 2.0e-29
Identity = 81/134 (60.45%), Postives = 88/134 (65.67%), Query Frame = 0


Query: 1   MAFSFSLPCSFKLALTVALLFFFSFCPSKSVGLFSSRRGKGE-----KEMKYLEEAETER 60
           MA   SL  S KLALT+A   FFSF P  SVGL  SR G+ E      EMK LE      
Sbjct: 3   MALPISLSTSLKLALTLAA--FFSFLPPTSVGLVLSRSGRSEGKADNDEMKILE------ 62

Query: 61  ESYVEDEKKMVIGSRPPGCENKCKSCRPCMAAVVVPVHRMK-------ASTREEEDSYYL 120
                  +KMVIGSRPPGC NKC +CRPCMAA VVPVHRMK       +S+REEEDSYYL
Sbjct: 63  ------NQKMVIGSRPPGCRNKCMNCRPCMAAAVVPVHRMKGKAFQSFSSSREEEDSYYL 122

Query: 121 LSWKCRCGNKIYQP 123
           LSWKC+CGNKIYQP
Sbjct: 123 LSWKCQCGNKIYQP 122

[Close]

BLAST of Lag0002909 vs. ExPASy Swiss-Prot
Match: Q9LUH9 (EPIDERMAL PATTERNING FACTOR-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=EPFL5 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 8.6e-07
Identity = 38/109 (34.86%), Postives = 55/109 (50.46%), Query Frame = 0


Query: 15  LTVALLFFFSFCPSKSVGLFSSRRGKGEKEMKYLEEAETERESYVEDEKKM-VIGSRPPG 74
           +  A L FFS   + S+   S   G+G+KE+     A +     + D+K++   GS PP 
Sbjct: 9   IVYAFLLFFSSSSAASLQRPSGGLGQGKKEI-----ARSGLPGQIVDQKRLGGPGSVPPM 68

Query: 75  CENKCKSCRPCMAAVVVPVHRMKASTREEEDSYYLLSWKCRCGNKIYQP 123
           C  KC  C PC  AV VP+        E    YY  +W+C+CGNK++ P
Sbjct: 69  CRLKCGKCEPC-KAVHVPIQPGLIMPLE----YYPEAWRCKCGNKLFMP 107

[Close]

BLAST of Lag0002909 vs. ExPASy Swiss-Prot
Match: Q1PEY6 (EPIDERMAL PATTERNING FACTOR-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=EPFL6 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.5e-06
Identity = 26/56 (46.43%), Postives = 32/56 (57.14%), Query Frame = 0


Query: 67  IGSRPPGCENKCKSCRPCMAAVVVPVHRMKASTREEEDSYYLLSWKCRCGNKIYQP 123
           +GS PP C +KC  C PC   V VPV      T E    YY  +W+C+CGNK+Y P
Sbjct: 106 LGSSPPRCSSKCGRCTPC-KPVHVPVPPGTPVTAE----YYPEAWRCKCGNKLYMP 156

[Close]

BLAST of Lag0002909 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.5e-06
Identity = 25/59 (42.37%), Postives = 32/59 (54.24%), Query Frame = 0


Query: 64  KMVIGSRPPGCENKCKSCRPCMAAVVVPVHRMKASTREEEDSYYLLSWKCRCGNKIYQP 123
           ++++GSRPP C NKC  C PC A +V+ VHR+          YY   W C    KIY P
Sbjct: 463 RILLGSRPPNCTNKCHGCSPCKAKLVI-VHRIM------PQEYYPQRWMCSRHGKIYHP 514

[Close]

BLAST of Lag0002909 vs. ExPASy Swiss-Prot
Match: Q1G3V9 (EPIDERMAL PATTERNING FACTOR-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=EPFL8 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.9e-06
Identity = 21/62 (33.87%), Postives = 40/62 (64.52%), Query Frame = 0


Query: 63  KKMVIGSRPPGCENKCKSCRPCMAAVVVPVHRMKASTREEEDS--YYLLSWKCRCGNKIY 122
           K+ V+GS PP C  KC++C+PC+      +  ++ +  +++DS  YY + W CRC ++++
Sbjct: 42  KESVMGSEPPVCATKCRNCKPCLPY----LFDIRGAHDDDDDSEPYYPVKWICRCRDRVF 99

[Close]

BLAST of Lag0002909 vs. ExPASy Swiss-Prot
Match: Q2V3I3 (EPIDERMAL PATTERNING FACTOR-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=EPFL4 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 9.5e-06
Identity = 22/55 (40.00%), Postives = 29/55 (52.73%), Query Frame = 0


Query: 68  GSRPPGCENKCKSCRPCMAAVVVPVHRMKASTREEEDSYYLLSWKCRCGNKIYQP 123
           GS PP C +KC  C+PC      PVH            YY  +W+C+CGNK++ P
Sbjct: 60  GSSPPTCRSKCGKCQPCK-----PVHVPIQPGLSMPLEYYPEAWRCKCGNKLFMP 109

The following BLAST results are available for this feature:

BLAST of Lag0002909 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6593393.1	3.3e-32	68.80	Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7025740.1	5.7e-32	67.72	Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAE8646441.1	1.4e-30	62.79	hypothetical protein Csa_015844 [Cucumis sativus]	[more]
XP_008461115.1	1.8e-30	61.24	PREDICTED: EPIDERMAL PATTERNING FACTOR-like protein 4 [Cucumis melo]	[more]
KAA0058756.1	2.0e-29	60.45	EPIDERMAL PATTERNING FACTOR-like protein 4 [Cucumis melo var. makuwa] >TYK10550....	[more]

BLAST of Lag0002909 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LUH9	8.6e-07	34.86	EPIDERMAL PATTERNING FACTOR-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=EP...	[more]
Q1PEY6	1.5e-06	46.43	EPIDERMAL PATTERNING FACTOR-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=EP...	[more]
P43212	1.5e-06	42.37	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q1G3V9	1.9e-06	33.87	EPIDERMAL PATTERNING FACTOR-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=EP...	[more]
Q2V3I3	9.5e-06	40.00	EPIDERMAL PATTERNING FACTOR-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=EP...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF17181	EPF	coord: 66..122 e-value: 4.1E-21 score: 74.7
None	No IPR available	PANTHER	PTHR33109:SF57	EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN	coord: 9..122
IPR039455	EPIDERMAL PATTERNING FACTOR-like protein	PANTHER	PTHR33109	EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 4	coord: 9..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002909.1	Lag0002909.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010052	guard cell differentiation
biological_process	GO:0010374	stomatal complex development
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane