Lag0002902 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002902
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cucumber peeling cupredoxin-like
Location	chr4: 46580066 .. 46581153 (-)
RNA-Seq Expression	Lag0002902
Synteny	Lag0002902

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGGCGGAGTTGCTTTGGTTTTGGCTTTAATTGCGGTGGTTTTTGTTCAACATGCCGCCGCTCAGAGGGAGCTTGTTGTCGGAGACTCCACCGGCTGGATGGTTCCGCAGGGCAACCCGACTCTGTATTCCGACTGGGCTGCTAGAAACAGCTTCGCCGTTGGCGATTCTCTCAGTAAGATTTTTTTCTTCTCTTCATCTTCTTAAATATAGATTATTATTATATTCTTAATTTCCTTCTTCATTTTTAAGATCTTTTTTAATTTTACTTCTAATGTTTTAAAAAAATTAAATAGAAACAACCCTTACGTTTGTGTCTATTTTAGTCTCAAACTTTCAAAAAGTTTCTCAAAATTTCCTGAACTCTCAATTTTGTGTCCAGAATGTTTTCGAGCTTTAAAAAAAAACTCTCAATTTTTGTGTCCAGAATCTTCCTCAACTTTAAAGAAAATTTTCTATTTTGTATCTAATAAGTTTTAAATTTTCAAGTTTAGGTTTAATAGATCACTGACATATTTTTAAAAATTCACATCATCTACAATTGAATTACCCAAAAGTCATTTTGGCTACTGTCGATGATTTTTTTTTAGAATATACTCTATCACAAATACAAGATGGGAATTGAAACGTAGAAACATAGACTTCTTCAAATTAGAAGTAGAGATGTCTTAATCATTTAACTATATCGAAGTAGGCAATAACAATAATTTGTAATTGAGTTCAAATACTTTATTTTTACAGGATTTAACTTTGAAACCGGCCGACATGACGTATTTGAAGTATCAAAAGAATCGTTCGACGCATGTCGTTTCGACAACACTGAAGATGACATCATCAGGACAGGTCCCGCGACGGTGCGTCTCAACGAGACAGGAATGCATTACTTTATTTGCACCATCGGACAACACTGTTTGGGAGGCCAAAAGTTATCCATCAATGTCGTAGCCACTTCGGCCGGTGGTCCGATGTCGCCTTCTTCACCGCAGCCGCCTTCTTCTCTACCACCACCATCGCCTTCCTCCTCTAGTGCAGTGATGGCTAGCCTTTATGTCACTTTGTCCGCCATTGTTATGAGCTTGTTTTAG

mRNA sequence

ATGGGCGGCGGAGTTGCTTTGGTTTTGGCTTTAATTGCGGTGGTTTTTGTTCAACATGCCGCCGCTCAGAGGGAGCTTGTTGTCGGAGACTCCACCGGCTGGATGGTTCCGCAGGGCAACCCGACTCTGTATTCCGACTGGGCTGCTAGAAACAGCTTCGCCGTTGGCGATTCTCTCAGATTTAACTTTGAAACCGGCCGACATGACGTATTTGAAGTATCAAAAGAATCGTTCGACGCATGTCGTTTCGACAACACTGAAGATGACATCATCAGGACAGGTCCCGCGACGGTGCGTCTCAACGAGACAGGAATGCATTACTTTATTTGCACCATCGGACAACACTGTTTGGGAGGCCAAAAGTTATCCATCAATGTCGTAGCCACTTCGGCCGGTGGTCCGATGTCGCCTTCTTCACCGCAGCCGCCTTCTTCTCTACCACCACCATCGCCTTCCTCCTCTAGTGCAGTGATGGCTAGCCTTTATGTCACTTTGTCCGCCATTGTTATGAGCTTGTTTTAG

Coding sequence (CDS)

ATGGGCGGCGGAGTTGCTTTGGTTTTGGCTTTAATTGCGGTGGTTTTTGTTCAACATGCCGCCGCTCAGAGGGAGCTTGTTGTCGGAGACTCCACCGGCTGGATGGTTCCGCAGGGCAACCCGACTCTGTATTCCGACTGGGCTGCTAGAAACAGCTTCGCCGTTGGCGATTCTCTCAGATTTAACTTTGAAACCGGCCGACATGACGTATTTGAAGTATCAAAAGAATCGTTCGACGCATGTCGTTTCGACAACACTGAAGATGACATCATCAGGACAGGTCCCGCGACGGTGCGTCTCAACGAGACAGGAATGCATTACTTTATTTGCACCATCGGACAACACTGTTTGGGAGGCCAAAAGTTATCCATCAATGTCGTAGCCACTTCGGCCGGTGGTCCGATGTCGCCTTCTTCACCGCAGCCGCCTTCTTCTCTACCACCACCATCGCCTTCCTCCTCTAGTGCAGTGATGGCTAGCCTTTATGTCACTTTGTCCGCCATTGTTATGAGCTTGTTTTAG

Protein sequence

MGGGVALVLALIAVVFVQHAAAQRELVVGDSTGWMVPQGNPTLYSDWAARNSFAVGDSLRFNFETGRHDVFEVSKESFDACRFDNTEDDIIRTGPATVRLNETGMHYFICTIGQHCLGGQKLSINVVATSAGGPMSPSSPQPPSSLPPPSPSSSSAVMASLYVTLSAIVMSLF

Homology

[Close]

BLAST of Lag0002902 vs. NCBI nr
Match: XP_038896386.1 (cucumber peeling cupredoxin-like [Benincasa hispida])

HSP 1 Score: 221.1 bits (562), Expect = 7.5e-54
Identity = 114/175 (65.14%), Postives = 137/175 (78.29%), Query Frame = 0


Query: 1   MGGGVALVLALIAVVFVQHAAAQRELVVGDSTGWMV-PQGNPTLYSDWAARNSFAVGDSL 60
           M  GV  VL L+A+VF+ HA AQ    VG +TGW V P G P  YSDWAA   F VGDSL
Sbjct: 1   MAAGVGFVLGLVALVFLHHATAQTVHDVGGTTGWTVPPSGGPAFYSDWAANKIFRVGDSL 60

Query: 61  RFNFETGRHDVFEVSKESFDACRFDNTEDDIIRTGPATVRLNETGMHYFICTIGQHCLGG 120
           RFNF+T  HDVFEVSKESFD+C F+ T+DDIIR GPATV LN+T MHYF+CT+G HC  G
Sbjct: 61  RFNFQTNNHDVFEVSKESFDSCTFNPTDDDIIRAGPATVTLNDTEMHYFVCTVGPHCSLG 120

Query: 121 QKLSINVVATSAGGPMSPSSPQPPSS-LPPPSPSSSSAVMASLYVTLSAIVMSLF 174
           QKLSINVV+ +AGGP+SPSSP PPS+ LPPPSPSSS+++ A+LY++ SAI+M+ F
Sbjct: 121 QKLSINVVSAAAGGPISPSSPAPPSTVLPPPSPSSSNSLSATLYLSFSAILMTFF 175

[Close]

BLAST of Lag0002902 vs. NCBI nr
Match: KAG7014139.1 (hypothetical protein SDJN02_24312, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 208.0 bits (528), Expect = 6.6e-50
Identity = 109/167 (65.27%), Postives = 129/167 (77.25%), Query Frame = 0


Query: 8   VLALIAVVFVQHAAAQRELVVGDSTGWMVPQGNPTLYSDWAARNSFAVGDSLRFNFETGR 67
           +  L+AV+F+QHA AQ    VG STGW VP G    YSDWAARN+F+VGDSLRF F TG 
Sbjct: 13  LFCLLAVLFLQHATAQTTHDVGGSTGWTVPTGGAAFYSDWAARNTFSVGDSLRFAFATGS 72

Query: 68  HDVFEV-SKESFDACRFDNTEDDIIRTGPATVRLNETGMHYFICTIGQHCLGGQKLSINV 127
           H V +V SKESF+ CRF+N + DI+ TGPATV LN TGMHYFICTIG HC  GQK++INV
Sbjct: 73  HSVLKVSSKESFEGCRFENGDSDIMTTGPATVALNSTGMHYFICTIGTHCSLGQKVAINV 132

Query: 128 VATSAGGPMSPSSPQPPSSLPPPSPSSSSAVMASLYVTLSAIVMSLF 174
            AT+AGGPMSP  P P S  PPPSPSS++AV A+LY+T SA+VM+LF
Sbjct: 133 GATAAGGPMSP--PMPASQSPPPSPSSANAVTATLYLTFSAVVMALF 177

[Close]

BLAST of Lag0002902 vs. NCBI nr
Match: BAI52948.1 (blue copper protein precursor [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 207.2 bits (526), Expect = 1.1e-49
Identity = 111/174 (63.79%), Postives = 130/174 (74.71%), Query Frame = 0


Query: 1   MGGGVALVLALIAVVFVQHAAAQRELVVGDSTGWMVPQGNPTLYSDWAARNSFAVGDSLR 60
           M  G+  VL  +AVVF+QHA AQ   VVGD+TGW VPQ  P  YS WAA  +F VGDSL 
Sbjct: 1   MAAGLGFVLGFLAVVFLQHATAQTVHVVGDNTGWTVPQDGPAFYSGWAANKNFRVGDSLT 60

Query: 61  FNFETGRHDVFEVSKESFDACRFDNTEDDIIRTGPATVRLNETGMHYFICTIGQHCLGGQ 120
           FNF+TG HDV +VSKESFD C F   +DDIIRTGP TVRL+ET MHYF  TI  HC  GQ
Sbjct: 61  FNFQTGSHDVLKVSKESFDRCNFTGDDDDIIRTGPTTVRLHETDMHYFYWTIRTHCSLGQ 120

Query: 121 KLSINVVATSAGGPMSPSSPQPPSSL-PPPSPSSSSAVMASLYVTLSAIVMSLF 174
           KLSINVVA +AGGPMSPS   PPSS+ PPPSPSSS+A++A+L +  SA++M+ F
Sbjct: 121 KLSINVVAATAGGPMSPS---PPSSVFPPPSPSSSNALIATLCLNFSALLMAFF 171

[Close]

BLAST of Lag0002902 vs. NCBI nr
Match: KAG6575597.1 (hypothetical protein SDJN03_26236, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 206.5 bits (524), Expect = 1.9e-49
Identity = 108/167 (64.67%), Postives = 128/167 (76.65%), Query Frame = 0


Query: 8   VLALIAVVFVQHAAAQRELVVGDSTGWMVPQGNPTLYSDWAARNSFAVGDSLRFNFETGR 67
           +  L+AV+F+QHA AQ    VG STGW VP G    YSDWAARN+F+VGDSLRF F TG 
Sbjct: 11  LFCLLAVLFLQHATAQTTHDVGGSTGWTVPTGGAAFYSDWAARNTFSVGDSLRFAFATGS 70

Query: 68  HDVFEV-SKESFDACRFDNTEDDIIRTGPATVRLNETGMHYFICTIGQHCLGGQKLSINV 127
           H V +V SKESF+ CRF+N + DI+ TGP TV LN TGMHYFICTIG HC  GQK++INV
Sbjct: 71  HSVLKVSSKESFEGCRFENGDSDIMTTGPVTVALNSTGMHYFICTIGTHCSLGQKVAINV 130

Query: 128 VATSAGGPMSPSSPQPPSSLPPPSPSSSSAVMASLYVTLSAIVMSLF 174
            AT+AGGPMSP  P P S  PPPSPSS++AV A+LY+T SA+VM+LF
Sbjct: 131 GATAAGGPMSP--PMPASQSPPPSPSSANAVTATLYLTFSAVVMALF 175

[Close]

BLAST of Lag0002902 vs. NCBI nr
Match: XP_022151423.1 (cucumber peeling cupredoxin-like [Momordica charantia])

HSP 1 Score: 201.1 bits (510), Expect = 8.0e-48
Identity = 113/197 (57.36%), Postives = 130/197 (65.99%), Query Frame = 0


Query: 2   GGGVALVLALIAVVFVQHAAAQRELVVGDSTGWMVPQGNPTLYSDWAARNSFAVGDSLRF 61
           GG +ALVL  +A V  Q AAAQ   VVGD+TGW VPQG  T YSDWAAR +F  GDSL F
Sbjct: 3   GGVIALVLGFVAAVLAQQAAAQTVHVVGDATGWTVPQGGATFYSDWAARRNFVAGDSLTF 62

Query: 62  NFETGRHDVFEVSKESFDACRFDNTEDDIIRTGPATVRLNETGMHYFICTIGQHCLGGQK 121
            F T  HDV EV+KESFDAC  DN   ++I TGPATV+LN  G+HY+ICT+G+HCLGGQK
Sbjct: 63  TFGTNAHDVLEVTKESFDACSSDNAIGNVITTGPATVKLNAAGVHYYICTVGRHCLGGQK 122

Query: 122 LSINVVATSAGGPMSPSSPQP-------------------PSSLPP--------PSPSSS 172
           LSI V AT  G   SP++P+P                   PSS PP        P PSSS
Sbjct: 123 LSITVSATPGGAGPSPNTPRPPPATTPSHANDDACAPAGSPSSSPPNGGGSQSAPPPSSS 182

[Close]

BLAST of Lag0002902 vs. ExPASy Swiss-Prot
Match: P29602 (Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3)

HSP 1 Score: 146.7 bits (369), Expect = 2.4e-34
Identity = 75/133 (56.39%), Postives = 93/133 (69.92%), Query Frame = 0


Query: 27  VVGDSTGWMVPQGNPTLYSDWAARNSFAVGDSLRFNFETGRHDVFEV-SKESFDACRFDN 86
           +VGD+TGW VP  +P  YS WAA  +F VGDSL+FNF    H+V E+ +K+SFDAC F N
Sbjct: 6   IVGDNTGWSVP-SSPNFYSQWAAGKTFRVGDSLQFNFPANAHNVHEMETKQSFDACNFVN 65

Query: 87  TEDDIIRTGPATVRLNETGMHYFICTIGQHCLGGQKLSINVVATSAGGPMSPSSPQPPSS 146
           +++D+ RT P   RL+E GMHYF+CT+G HC  GQKLSINVVA +A   M P S  PPSS
Sbjct: 66  SDNDVERTSPVIERLDELGMHYFVCTVGTHCSNGQKLSINVVAANATVSMPPPSSSPPSS 125

Query: 147 ------LPPPSPS 153
                 +PPPSPS
Sbjct: 126 VMPPPVMPPPSPS 137

[Close]

BLAST of Lag0002902 vs. ExPASy Swiss-Prot
Match: Q07488 (Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 2.1e-19
Identity = 58/152 (38.16%), Postives = 78/152 (51.32%), Query Frame = 0


Query: 4   GVALVLALIAVVFVQHAAAQRELVVGDSTGWMVPQGNPTLYSDWAARNSFAVGDSLRFNF 63
           GV   +  + +VF        +  VGD T W  P  +P  Y+ WA   +F VGD L F+F
Sbjct: 3   GVFKTVTFLVLVFAAVVVFAEDYDVGDDTEWTRPM-DPEFYTTWATGKTFRVGDELEFDF 62

Query: 64  ETGRHDVFEVSKESFDACRFDNTEDDIIRTGPATVRLNETGMHYFICTIGQHCLGGQKLS 123
             GRHDV  VS+ +F+ C  +      +   P  + LN TG  YFICT+G HC  GQKLS
Sbjct: 63  AAGRHDVAVVSEAAFENCEKEKPISH-MTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLS 122

Query: 124 INVVATSAGGPMSPSSPQPPSSLPPPSPSSSS 156
           I VVA  A G  +P +   P+    PS   ++
Sbjct: 123 ITVVAAGATGGATPGAGATPAPGSTPSTGGTT 152

[Close]

BLAST of Lag0002902 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.1e-19
Identity = 52/113 (46.02%), Postives = 66/113 (58.41%), Query Frame = 0


Query: 28  VGDSTGWMVPQGNPTLYSDWAARNSFAVGDSLRFNFETGRHDVFEVSKESFDACRFDNTE 87
           VG    W  P  +P  Y  WA   +F VGD L F+F  G HDV  V+K++FD C+ +N  
Sbjct: 5   VGGDMEWKRP-SDPKFYITWATGKTFRVGDELEFDFAAGMHDVAVVTKDAFDNCKKENPI 64

Query: 88  DDIIRTGPATVRLNETGMHYFICTIGQHCLGGQKLSINVV-ATSAGGPMSPSS 140
              + T P  + LN TG  Y+ICT+G HC  GQKLSINVV A  AGG  +P +
Sbjct: 65  SH-MTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINVVGAGGAGGGATPGA 115

[Close]

BLAST of Lag0002902 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.9e-16
Identity = 63/160 (39.38%), Postives = 86/160 (53.75%), Query Frame = 0


Query: 6   ALVLA-LIAVVFVQHAAAQRELVVGDSTGWMVPQGNPTLYSDWAARNSFAVGDSLRFNFE 65
           ALVL  L+A++ +   +      VGD++GW++       YS WA+  +FAVGDSL FN+ 
Sbjct: 6   ALVLCFLLAIINMALPSLATVYTVGDTSGWVIGGD----YSTWASDKTFAVGDSLVFNYG 65

Query: 66  TGRHDVFEVSKESFDACRFDNTEDDIIRTGPATVRLNETGMHYFICTIGQHCLGGQKLSI 125
            G H V EV +  + +C   N+      TG  T+ L + G HYFIC +  H  GG KLSI
Sbjct: 66  AGAHTVDEVKESDYKSCTSGNS-ISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSI 125

Query: 126 NVVATSAGGPMSPSSPQPPSSLPPPSPSSSSAVMASLYVT 165
            V A S+G   +PS+   PSS    SPSS     A+   T
Sbjct: 126 KVKA-SSGSSAAPSA--TPSSSGKGSPSSDDTPAATTTTT 157

[Close]

BLAST of Lag0002902 vs. ExPASy Swiss-Prot
Match: Q9SK27 (Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 3.9e-13
Identity = 52/167 (31.14%), Postives = 87/167 (52.10%), Query Frame = 0


Query: 6   ALVLALIAVVFVQHAAAQRELVVGDSTG-WMVPQGNPTLYSDWAARNSFAVGDSLRFNFE 65
           +L +A+ +++F+   AA  E+ VG  +G W +P  +   +++WA +  F VGD + F +E
Sbjct: 11  SLHVAIFSLIFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYE 70

Query: 66  TGRHDVFEVSKESFDACRFDNTEDDIIRTGPATVRLNETGMHYFICTIGQHCLGGQKLSI 125
           +G+  V EV+KE++++C   N   +    G   V+L+ +G  YFI     HC  GQKLS+
Sbjct: 71  SGKDSVLEVTKEAYNSCNTTNPLAN-YTDGETKVKLDRSGPFYFISGANGHCEKGQKLSL 130

Query: 126 NVVATSAGGPMSPSSPQPPSSLP--PPSPSSSSAVMASLYVTLSAIV 170
            V++          SP      P   P+P S S  +   YV L  ++
Sbjct: 131 VVISPRHSVISPAPSPVEFEDGPALAPAPISGSVRLGGCYVVLGLVL 176

The following BLAST results are available for this feature:

BLAST of Lag0002902 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896386.1	7.5e-54	65.14	cucumber peeling cupredoxin-like [Benincasa hispida]	[more]
KAG7014139.1	6.6e-50	65.27	hypothetical protein SDJN02_24312, partial [Cucurbita argyrosperma subsp. argyro...	[more]
BAI52948.1	1.1e-49	63.79	blue copper protein precursor [Citrullus lanatus subsp. vulgaris]	[more]
KAG6575597.1	1.9e-49	64.67	hypothetical protein SDJN03_26236, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022151423.1	8.0e-48	57.36	cucumber peeling cupredoxin-like [Momordica charantia]	[more]

BLAST of Lag0002902 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P29602	2.4e-34	56.39	Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3	[more]
Q07488	2.1e-19	38.16	Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2	[more]
P42849	2.1e-19	46.02	Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1	[more]
Q41001	4.9e-16	39.38	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
Q9SK27	3.9e-13	31.14	Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 34..120 e-value: 2.3E-21 score: 75.6
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 24..128 score: 37.541161
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 22..130 e-value: 9.9E-34 score: 117.8
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 25..127
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 131..151
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 136..150
None	No IPR available	PANTHER	PTHR33021:SF190	UMECYANIN-LIKE	coord: 5..158
None	No IPR available	CDD	cd13920	Stellacyanin	coord: 27..126 e-value: 6.06928E-48 score: 149.385
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 5..158
IPR028871	Blue (type 1) copper protein, binding site	PROSITE	PS00196	COPPER_BLUE	coord: 104..120

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002902.1	Lag0002902.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0009055	electron transfer activity