Lag0002842 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002842
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAspartyl protease family protein
Locationchr4: 46162631 .. 46164227 (+)
RNA-Seq ExpressionLag0002842
SyntenyLag0002842
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATTGCAAAATCTCTTCATTCTCCTATTCTCCTCCTCCTTCTTCTCCTTCTCCTCTTCGCGGTCGACTCTCGTTCGATCGGCGCCCGTAATCGCCATCAGAATAAGAAGCTTCTTCTTAACCTTCGGAACCTTCCATGGAGCCAGAATAAGAAAAGAGCACCAACTGATTGCACCTTTCAGAAGCCAAGTGAGTGTTCCAAAAAAATCCCATTAAAAAATGAGGGAAAATTCTCTCTTAATATTAATCTGCATTCTTGTTATATTAATTAATTCTGGGATCTGTTTTTTTTTTTTTTTTTTTTTTTGAATTGCAGAGGTGGAGAATGGAGCAACCACATTGCAAATGAAAGAAAGAGACGACTGTTCAGGCAAAGTCATAGATTGGGACGAGAATCTCCAGAACCGCGTACTCCGCGACGCCATTCACGTCAAAGCCCTCCAATCCCGAGTCAGATCCGCCATTTCTCACAACAAACCCCTGGGAATCTCCGAGTCCCAAATCCCCATATCCCTCGGCCAGCCCTTCCAAACCCTCAACTACGTCGTCACCGTCGGAATCGGCGGCCAGAATTTAACCCTCATCGTCGACACCGGCAGCGACATCACCTGGGTCCAGTGCCGCCCTTGCCGCAAATGTTACACCCAACAAGAACCCATTTTCGATCCCTCAAATTCCACTTCCTTCCTCTCCCTTCCTTGCAATTCCACCACCTGCTCCTCCCTCGTCTACGGCCACTGTGCGCCTGGGACTAAGAATTCAAGCCCCTGTAATTATGAGATTAGCTATGGCGATGGATCTTATTCCAAAGGGGACTTTGCATACGAGAGGCTGACTTTGGGGATGATTACCGTCGACAAGTTTATAATTGGATGTGGTCAAACTAATGAGGGCTTGTACGGCGAAACTTCTGGGTTGATGGGTTTCGACAGAGATAAATTATCTATCGTTTCTCAAACATCCTCTGTTTTTGGTGGGATTTTTTCTTACTGTTTGCCTGCAACAGGGGATGGAACTTCAGGTTCTTTAACGATGGGGGGTGGAGATTTTTCCAGTTTCAGAAACGTCTCTCCAATTTCCTACACTAGAATGATCTCAGATCCTGAGATGCCGAGTTTTTACCTTTTGAACGTGACCGGAATTAGCATCGGCGGGGTGAAATTGAACACCCCGCGTTTCGCTTCTTCGGGTAATGGGGTTACGAGTTTACTCGATTCTGGAACTACTATTACCAGGCTGCCTCCTTCGGTTTACGATGCTTTCAAAGCGGAATTTGAGAAGCAATGTCCTGAATTGAGAAAAGTGCCAGGATATTCGATTTTGGAGGCTTGTTATAATCTTACAGAGGTTAAGGAATTCCAACTTCCGTCTGTGAAATTTTACTTTGAAGGTGATGCAGTGCTGACGGTTGATGTTCAAGGGATGCTTTACTTTTTGAAATCTGATTATTCTCAGACCTGTTTGGCGTTTGCGAGATTGTCTGGTGAGGATCAGTTGGGGATTATCGGGAGTTACCAGCAGAAGAATCAGAGGGTCCTTTATAATACCCAGGAATCGAAGGTGGGTTTTGCAGTGGAGACTTGCAGTTTCTAG

mRNA sequence

ATGGAGATTGCAAAATCTCTTCATTCTCCTATTCTCCTCCTCCTTCTTCTCCTTCTCCTCTTCGCGGTCGACTCTCGTTCGATCGGCGCCCGTAATCGCCATCAGAATAAGAAGCTTCTTCTTAACCTTCGGAACCTTCCATGGAGCCAGAATAAGAAAAGAGCACCAACTGATTGCACCTTTCAGAAGCCAAAGGTGGAGAATGGAGCAACCACATTGCAAATGAAAGAAAGAGACGACTGTTCAGGCAAAGTCATAGATTGGGACGAGAATCTCCAGAACCGCGTACTCCGCGACGCCATTCACGTCAAAGCCCTCCAATCCCGAGTCAGATCCGCCATTTCTCACAACAAACCCCTGGGAATCTCCGAGTCCCAAATCCCCATATCCCTCGGCCAGCCCTTCCAAACCCTCAACTACGTCGTCACCGTCGGAATCGGCGGCCAGAATTTAACCCTCATCGTCGACACCGGCAGCGACATCACCTGGGTCCAGTGCCGCCCTTGCCGCAAATGTTACACCCAACAAGAACCCATTTTCGATCCCTCAAATTCCACTTCCTTCCTCTCCCTTCCTTGCAATTCCACCACCTGCTCCTCCCTCGTCTACGGCCACTGTGCGCCTGGGACTAAGAATTCAAGCCCCTGTAATTATGAGATTAGCTATGGCGATGGATCTTATTCCAAAGGGGACTTTGCATACGAGAGGCTGACTTTGGGGATGATTACCGTCGACAAGTTTATAATTGGATGTGGTCAAACTAATGAGGGCTTGTACGGCGAAACTTCTGGGTTGATGGGTTTCGACAGAGATAAATTATCTATCGTTTCTCAAACATCCTCTGTTTTTGGTGGGATTTTTTCTTACTGTTTGCCTGCAACAGGGGATGGAACTTCAGGTTCTTTAACGATGGGGGGTGGAGATTTTTCCAGTTTCAGAAACGTCTCTCCAATTTCCTACACTAGAATGATCTCAGATCCTGAGATGCCGAGTTTTTACCTTTTGAACGTGACCGGAATTAGCATCGGCGGGGTGAAATTGAACACCCCGCGTTTCGCTTCTTCGGGTAATGGGGTTACGAGTTTACTCGATTCTGGAACTACTATTACCAGGCTGCCTCCTTCGGTTTACGATGCTTTCAAAGCGGAATTTGAGAAGCAATGTCCTGAATTGAGAAAAGTGCCAGGATATTCGATTTTGGAGGCTTGTTATAATCTTACAGAGGTTAAGGAATTCCAACTTCCGTCTGTGAAATTTTACTTTGAAGGTGATGCAGTGCTGACGGTTGATGTTCAAGGGATGCTTTACTTTTTGAAATCTGATTATTCTCAGACCTGTTTGGCGTTTGCGAGATTGTCTGGTGAGGATCAGTTGGGGATTATCGGGAGTTACCAGCAGAAGAATCAGAGGGTCCTTTATAATACCCAGGAATCGAAGGTGGGTTTTGCAGTGGAGACTTGCAGTTTCTAG

Coding sequence (CDS)

ATGGAGATTGCAAAATCTCTTCATTCTCCTATTCTCCTCCTCCTTCTTCTCCTTCTCCTCTTCGCGGTCGACTCTCGTTCGATCGGCGCCCGTAATCGCCATCAGAATAAGAAGCTTCTTCTTAACCTTCGGAACCTTCCATGGAGCCAGAATAAGAAAAGAGCACCAACTGATTGCACCTTTCAGAAGCCAAAGGTGGAGAATGGAGCAACCACATTGCAAATGAAAGAAAGAGACGACTGTTCAGGCAAAGTCATAGATTGGGACGAGAATCTCCAGAACCGCGTACTCCGCGACGCCATTCACGTCAAAGCCCTCCAATCCCGAGTCAGATCCGCCATTTCTCACAACAAACCCCTGGGAATCTCCGAGTCCCAAATCCCCATATCCCTCGGCCAGCCCTTCCAAACCCTCAACTACGTCGTCACCGTCGGAATCGGCGGCCAGAATTTAACCCTCATCGTCGACACCGGCAGCGACATCACCTGGGTCCAGTGCCGCCCTTGCCGCAAATGTTACACCCAACAAGAACCCATTTTCGATCCCTCAAATTCCACTTCCTTCCTCTCCCTTCCTTGCAATTCCACCACCTGCTCCTCCCTCGTCTACGGCCACTGTGCGCCTGGGACTAAGAATTCAAGCCCCTGTAATTATGAGATTAGCTATGGCGATGGATCTTATTCCAAAGGGGACTTTGCATACGAGAGGCTGACTTTGGGGATGATTACCGTCGACAAGTTTATAATTGGATGTGGTCAAACTAATGAGGGCTTGTACGGCGAAACTTCTGGGTTGATGGGTTTCGACAGAGATAAATTATCTATCGTTTCTCAAACATCCTCTGTTTTTGGTGGGATTTTTTCTTACTGTTTGCCTGCAACAGGGGATGGAACTTCAGGTTCTTTAACGATGGGGGGTGGAGATTTTTCCAGTTTCAGAAACGTCTCTCCAATTTCCTACACTAGAATGATCTCAGATCCTGAGATGCCGAGTTTTTACCTTTTGAACGTGACCGGAATTAGCATCGGCGGGGTGAAATTGAACACCCCGCGTTTCGCTTCTTCGGGTAATGGGGTTACGAGTTTACTCGATTCTGGAACTACTATTACCAGGCTGCCTCCTTCGGTTTACGATGCTTTCAAAGCGGAATTTGAGAAGCAATGTCCTGAATTGAGAAAAGTGCCAGGATATTCGATTTTGGAGGCTTGTTATAATCTTACAGAGGTTAAGGAATTCCAACTTCCGTCTGTGAAATTTTACTTTGAAGGTGATGCAGTGCTGACGGTTGATGTTCAAGGGATGCTTTACTTTTTGAAATCTGATTATTCTCAGACCTGTTTGGCGTTTGCGAGATTGTCTGGTGAGGATCAGTTGGGGATTATCGGGAGTTACCAGCAGAAGAATCAGAGGGTCCTTTATAATACCCAGGAATCGAAGGTGGGTTTTGCAGTGGAGACTTGCAGTTTCTAG

Protein sequence

MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKKRAPTDCTFQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISHNKPLGISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTSFLSLPCNSTTCSSLVYGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYERLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPRFASSGNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLPSVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNTQESKVGFAVETCSF
Homology
BLAST of Lag0002842 vs. NCBI nr
Match: KAG6575642.1 (Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 615.9 bits (1587), Expect = 3.0e-172
Identity = 317/495 (64.04%), Postives = 376/495 (75.96%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNK-KLLLNLRNLPWSQNKKRAPTDC 60
           MEI+KSL   +LLLLLLLLLF VD     A N    K   LL+L+ LPW Q ++ A  +C
Sbjct: 1   MEISKSLCFFLLLLLLLLLLFFVDQARSDAINGDSEKLHRLLHLQKLPWKQ-QEEAVINC 60

Query: 61  TFQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISHNKP 120
            FQKP+V  G TTL+MKERD CSGKV DW +NLQNR++ DAIHV++LQSR++SAI     
Sbjct: 61  IFQKPRVREGITTLEMKERDYCSGKVTDWQKNLQNRLISDAIHVQSLQSRIKSAIFSGDT 120

Query: 121 LGISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQEPI 180
             IS+SQIP+S G   QTLNY+VTV +GG++ TLIVDTGSD+TWVQCRPCR CY QQEP+
Sbjct: 121 HQISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPL 180

Query: 181 FDPSNSTSFLSLPCNSTTCSSLV-----YGHCAPGTKNSSPCNYEISYGDGSYSKGDFAY 240
           FDPSNS+SFLSL CNS TC +L       G C  G  NSS C YEI+YGDGSYS+G+  +
Sbjct: 181 FDPSNSSSFLSLSCNSPTCLALPPATGNSGLCGYG--NSSSCGYEINYGDGSYSRGELGF 240

Query: 241 ERLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPAT 300
           ERL LG I +D FI GCG+ N+GL+G  SGLMG  R KLS+VSQTSSVF GIFSYCLP+T
Sbjct: 241 ERLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPST 300

Query: 301 GDGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPRFAS 360
           G G SGSLTMGGGDFS+FRNVSPISYTRM+S+P+MP+FY LN+TGI+IGGV L       
Sbjct: 301 GAGASGSLTMGGGDFSNFRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV----- 360

Query: 361 SGNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQL 420
           S NG  SL+DSGT ITRL PS+Y AFKAEFEKQ    +  PG+SIL  C+NLT  KE  +
Sbjct: 361 SNNGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNI 420

Query: 421 PSVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYN 480
           P+VKFYFEG+A +TVDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKNQRV+YN
Sbjct: 421 PTVKFYFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYN 480

Query: 481 TQESKVGFAVETCSF 490
           ++ES VGFA E C F
Sbjct: 481 SKESTVGFAAEPCGF 487

BLAST of Lag0002842 vs. NCBI nr
Match: KAG7014194.1 (Aspartyl protease family protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 614.4 bits (1583), Expect = 8.6e-172
Identity = 313/494 (63.36%), Postives = 374/494 (75.71%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKKRAPTDCT 60
           MEI+KSL   +LLLLLLLL F   +RS       +    LL+L+ LPW Q ++ A  +C 
Sbjct: 1   MEISKSLCFFLLLLLLLLLFFVDQARSDAINGDSEKLHRLLHLQKLPWKQ-QEEAVINCI 60

Query: 61  FQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISHNKPL 120
           FQKP+V  G TTL+MKERD CSGKV DW +NLQNR++ DAIHV++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKERDYCSGKVTDWQKNLQNRLISDAIHVQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G   QTLNY+VTV +GG++ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSLPCNSTTCSSLV-----YGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYE 240
           DPSNS+SFLSL CNS TC +L       G C  G  NSS C YEI+YGDGSYS+G+  +E
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPPATGNSGLCGYG--NSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG I +D FI GCG+ N+GL+G  SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPRFASS 360
            G SGSLTMGGGDFS+FRNVSPISYTRM+S+P+MP+FY LN+TGI+IGGV L       S
Sbjct: 301 AGASGSLTMGGGDFSNFRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV-----S 360

Query: 361 GNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLP 420
            NG  SL+DSGT ITRL PS+Y AFKAEFEKQ    +  PG+SIL  C+NLT  KE  +P
Sbjct: 361 NNGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIP 420

Query: 421 SVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNT 480
           +VKFYFEG+A +TVDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKNQRV+YN+
Sbjct: 421 TVKFYFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNS 480

Query: 481 QESKVGFAVETCSF 490
           +ES VGFA E C F
Sbjct: 481 KESTVGFAAEPCGF 486

BLAST of Lag0002842 vs. NCBI nr
Match: XP_023547381.1 (aspartyl protease family protein At5g10770 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609.8 bits (1571), Expect = 2.1e-170
Identity = 311/494 (62.96%), Postives = 372/494 (75.30%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKKRAPTDCT 60
           MEI+KSL     LLLLLLL F   +RS       +    LL+L+ LPW Q ++ A  +C 
Sbjct: 1   MEISKSL--CFFLLLLLLLFFVDQARSAAINGDSEKLHRLLHLQKLPWKQ-QEEAVINCI 60

Query: 61  FQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISHNKPL 120
           FQKP+V  G TTL+MKERD CSGKV DW +NLQNR++ DAIHV++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKERDYCSGKVTDWQKNLQNRLISDAIHVQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G   QTLNY+VTV +GG++ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSLPCNSTTCSSLV-----YGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYE 240
           DPSNS+SFLSL CNS TC +L       G C  G  NSS C YEI+YGDGSYS+G+  +E
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPSATGNSGLCGYG--NSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG I +D FI GCG+ N+GL+G  SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPRFASS 360
            G SGSLTMGGGDFS+FRNVSPISYTRM+S+P+MP+FY LN+TGI+IGGV L  P     
Sbjct: 301 AGASGSLTMGGGDFSNFRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGVP----- 360

Query: 361 GNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLP 420
            NG  SL+DSGT ITRL PS+Y AFKAEFEKQ    +  PG+SIL  C+NLT  KE  +P
Sbjct: 361 NNGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIP 420

Query: 421 SVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNT 480
           +VKFYFEG+A +TVDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKNQRV+YN+
Sbjct: 421 TVKFYFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNS 480

Query: 481 QESKVGFAVETCSF 490
           +ES +GFA E C F
Sbjct: 481 KESTLGFAAEPCGF 484

BLAST of Lag0002842 vs. NCBI nr
Match: XP_022991886.1 (aspartyl protease family protein At5g10770 [Cucurbita maxima])

HSP 1 Score: 605.1 bits (1559), Expect = 5.2e-169
Identity = 309/494 (62.55%), Postives = 372/494 (75.30%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKKRAPTDCT 60
           MEI+KSL    LLLLLLLL F  ++RS       +    LL+L+ LPW Q ++ A  +C 
Sbjct: 1   MEISKSL-CFFLLLLLLLLFFVDEARSDAINGDSEKLHRLLHLQKLPWKQ-QEEAVVNCI 60

Query: 61  FQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISHNKPL 120
           FQKP+V  G TTL+MKERD CSGKV DW  NLQNR++ DAI +++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKERDYCSGKVTDWQNNLQNRLIFDAIRLQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G   QTLNY+VTV +GG++ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSLPCNSTTCSSLV-----YGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYE 240
           DPSNS+SFLSL CNS TC +L       G C  G  NSS C YEI+YGDGSYS+G+  +E
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPPATGNSGLC--GNGNSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG I +D FI GCG+ N+GL+G  SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPRFASS 360
            G SGSLTMGGGDFS++RNVSPISYTRM+S+P+MP+FY LN+TGI+IGGV L  P     
Sbjct: 301 AGASGSLTMGGGDFSNYRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGVP----- 360

Query: 361 GNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLP 420
            NG  SL+DSGT ITRL PS+Y AFKAEFEKQ    +  PG+SIL  C+NLT  KE  +P
Sbjct: 361 NNGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIP 420

Query: 421 SVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNT 480
           +VKF+FEG+A +TVDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKNQRV+YN+
Sbjct: 421 TVKFFFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNS 480

Query: 481 QESKVGFAVETCSF 490
           +ES VGFA E C F
Sbjct: 481 KESTVGFAAEPCGF 485

BLAST of Lag0002842 vs. NCBI nr
Match: XP_022953875.1 (aspartyl protease family protein At5g10770 [Cucurbita moschata])

HSP 1 Score: 602.8 bits (1553), Expect = 2.6e-168
Identity = 308/494 (62.35%), Postives = 370/494 (74.90%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKKRAPTDCT 60
           MEI+KSL      LLLLLL F   +RS       +    LL+L+  PW Q ++ A  +C 
Sbjct: 1   MEISKSL---CFFLLLLLLFFVDQARSDAINGDSEKLHRLLHLQKRPWKQ-QEEAVINCI 60

Query: 61  FQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISHNKPL 120
           FQKP+V  G TTL+MKE+D CSG+V DW +NLQNR++ DAIHV++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKEKDYCSGEVTDWQKNLQNRLISDAIHVQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G   QTLNY+VTV +GG++ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSLPCNSTTCSSLV-----YGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYE 240
           DPSNS+SFLSL CNS TC +L       G C  G  NSS C YEI+YGDGSYS+G+  +E
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPPATGNSGLC--GNGNSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG I +D FI GCG+ N+GL+G  SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPRFASS 360
            G SGSLTMGGGDFS+FRNVSPISYTRM+S+P+MP+FY LN+TGI+IGGV L       S
Sbjct: 301 AGASGSLTMGGGDFSNFRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV-----S 360

Query: 361 GNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLP 420
            NG  SL+DSGT ITRL PS+Y AFKAEFEKQ    +  PG+SIL  C+NLT  KE  +P
Sbjct: 361 NNGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIP 420

Query: 421 SVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNT 480
           +VKFYFEG+A +TVDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKNQRV+YN+
Sbjct: 421 TVKFYFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNS 480

Query: 481 QESKVGFAVETCSF 490
           +ES VGFA E C F
Sbjct: 481 KESTVGFAAEPCGF 483

BLAST of Lag0002842 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.1e-71
Identity = 185/498 (37.15%), Postives = 264/498 (53.01%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKKRAPTDCT 60
           M I ++L + I++L + L L   D    GA+ R  +   +     LP S       + C 
Sbjct: 1   MSINRNLLNIIIILCICLNLGCND----GAQERETDSHTIQVSSLLPSSS------SSCV 60

Query: 61  FQKPKVENGATTLQMKER-DDCS----GKVIDWDENLQNRVLR-DAIHVKALQSRVRSAI 120
              P+     ++L +  R   CS    GK    D      +LR D   V ++ S++   +
Sbjct: 61  L-SPRASTTKSSLHVTHRHGTCSRLNNGKATSPD---HVEILRLDQARVNSIHSKLSKKL 120

Query: 121 SHNKPLGISESQIPISLGQPFQTLNYVVTVGIG--GQNLTLIVDTGSDITWVQCRPC-RK 180
           + +       + +P   G    + NY+VTVG+G    +L+LI DTGSD+TW QC+PC R 
Sbjct: 121 ATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSLIFDTGSDLTWTQCQPCVRT 180

Query: 181 CYTQQEPIFDPSNSTSFLSLPCNSTTCSSLVYGHCAPGTKNSSPCNYEISYGDGSYSKGD 240
           CY Q+EPIF+PS STS+ ++ C+S  C SL       G+ ++S C Y I YGD S+S G 
Sbjct: 181 CYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCSASNCIYGIQYGDQSFSVGF 240

Query: 241 FAYERLTLGMITV-DKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYC 300
            A E+ TL    V D    GCG+ N+GL+   +GL+G  RDKLS  SQT++ +  IFSYC
Sbjct: 241 LAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRDKLSFPSQTATAYNKIFSYC 300

Query: 301 LPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTP 360
           LP++   T G LT G    S     +PIS    I+D    SFY LN+  I++GG KL  P
Sbjct: 301 LPSSASYT-GHLTFGSAGISRSVKFTPIS---TITDG--TSFYGLNIVAITVGGQKLPIP 360

Query: 361 RFASSGNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVK 420
               S  G  +L+DSGT ITRLPP  Y A ++ F+ +  +     G SIL+ C++L+  K
Sbjct: 361 STVFSTPG--ALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTSGVSILDTCFDLSGFK 420

Query: 421 EFQLPSVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQR 480
              +P V F F G AV+ +  +G+ Y  K   SQ CLAFA  S +    I G+ QQ+   
Sbjct: 421 TVTIPKVAFSFSGGAVVELGSKGIFYVFK--ISQVCLAFAGNSDDSNAAIFGNVQQQTLE 474

Query: 481 VLYNTQESKVGFAVETCS 489
           V+Y+    +VGFA   CS
Sbjct: 481 VVYDGAGGRVGFAPNGCS 474

BLAST of Lag0002842 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.6e-62
Identity = 136/406 (33.50%), Postives = 211/406 (51.97%), Query Frame = 0

Query: 92  LQNRVLRDAIHVKALQSRVRSAI--SHNKPLGISESQIPISLGQPFQTLNYVVTVGIGG- 151
           L  R+ RD   V A+  R+   +  S +    +++    I  G    +  Y V +G+G  
Sbjct: 81  LHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSP 140

Query: 152 -QNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTSFLSLPCNSTTCSSLVYGHCA 211
            ++  +++D+GSD+ WVQC+PC+ CY Q +P+FDP+ S S+  + C S+ C  +    C 
Sbjct: 141 PRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGC- 200

Query: 212 PGTKNSSPCNYEISYGDGSYSKGDFAYERLTLGMITVDKFIIGCGQTNEGLYGETSGLMG 271
               +S  C YE+ YGDGSY+KG  A E LT     V    +GCG  N G++   +GL+G
Sbjct: 201 ----HSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLG 260

Query: 272 FDRDKLSIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPI--SYTRMIS 331
                +S V Q S   GG F YCL + G  ++GSL  G       R   P+  S+  ++ 
Sbjct: 261 IGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFG-------REALPVGASWVPLVR 320

Query: 332 DPEMPSFYLLNVTGISIGGVKLNTP----RFASSGNGVTSLLDSGTTITRLPPSVYDAFK 391
           +P  PSFY + + G+ +GGV++  P        +G+G   ++D+GT +TRLP + Y AF+
Sbjct: 321 NPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV-VMDTGTAVTRLPTAAYVAFR 380

Query: 392 AEFEKQCPELRKVPGYSILEACYNLTEVKEFQLPSVKFYFEGDAVLTVDVQGMLYFLKSD 451
             F+ Q   L +  G SI + CY+L+     ++P+V FYF    VLT+  +  L  +  D
Sbjct: 381 DGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPV-DD 440

Query: 452 YSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNTQESKVGFAVETC 488
               C AFA  +    L IIG+ QQ+  +V ++     VGF    C
Sbjct: 441 SGTYCFAFA--ASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Lag0002842 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 7.8e-59
Identity = 139/408 (34.07%), Postives = 221/408 (54.17%), Query Frame = 0

Query: 86  IDWDENLQNRVLRDAIHVKALQSRVRSAISHNKPLGISESQIPISLGQPFQTLNYVVTVG 145
           +D DE ++    RD   V+++ S++ S  S N+      +++P   G    + NY+VT+G
Sbjct: 83  VDHDEIIR----RDQARVESIYSKL-SKNSANEVSEAKSTELPAKSGITLGSGNYIVTIG 142

Query: 146 IG--GQNLTLIVDTGSDITWVQCRPC-RKCYTQQEPIFDPSNSTSFLSLPCNSTTCSSLV 205
           IG    +L+L+ DTGSD+TW QC PC   CY+Q+EP F+PS+S+++ ++ C+S  C    
Sbjct: 143 IGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCED-- 202

Query: 206 YGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYERLTL-GMITVDKFIIGCGQTNEGLYGE 265
                  + ++S C Y I YGD S+++G  A E+ TL     ++    GCG+ N+GL+  
Sbjct: 203 -----AESCSASNCVYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDG 262

Query: 266 TSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPISYT 325
            +GL+G    KLS+ +QT++ +  IFSYCLP+    ++G LT G    S     +PIS  
Sbjct: 263 VAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNSTGHLTFGSAGISESVKFTPIS-- 322

Query: 326 RMISDPEMPSF--YLLNVTGISIGGVKLNTPRFASSGNGVTSLLDSGTTITRLPPSVYDA 385
                   PS   Y +++ GIS+G  +L     + S  G  +++DSGT  TRLP  VY  
Sbjct: 323 ------SFPSAFNYGIDIIGISVGDKELAITPNSFSTEG--AIIDSGTVFTRLPTKVYAE 382

Query: 386 FKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLPSVKFYFEGDAVLTVDVQGMLYFLK 445
            ++ F+++    +   GY + + CY+ T +     P++ F F G  V+ +D  G+   +K
Sbjct: 383 LRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPIK 442

Query: 446 SDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNTQESKVGFAVETC 488
              SQ CLAFA    +D   I G+ QQ    V+Y+    +VGFA   C
Sbjct: 443 --ISQVCLAFA--GNDDLPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of Lag0002842 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 7.8e-59
Identity = 139/414 (33.57%), Postives = 218/414 (52.66%), Query Frame = 0

Query: 89  DENLQNRVLRDAIHVKALQSRVRSAISHN-----KPLGISESQIPISLGQPFQTLNYVVT 148
           DE   +R+ RD+  VK++ +        N     +P G S S +    G    +  Y   
Sbjct: 89  DELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVS---GLSQGSGEYFTR 148

Query: 149 VGIG--GQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTSFLSLPCNSTTCSSL 208
           +G+G   + + +++DTGSDI W+QC PCR+CY+Q +PIFDP  S ++ ++PC+S  C  L
Sbjct: 149 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRL 208

Query: 209 VYGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYERLTLGMITVDKFIIGCGQTNEGLYGE 268
               C    K    C Y++SYGDGS++ GDF+ E LT     V    +GCG  NEGL+  
Sbjct: 209 DSAGCNTRRKT---CLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVG 268

Query: 269 TSGLMGFDRDKLSIVSQTSSVFGGIFSYCL-PATGDGTSGSLTMGGGDFSSFRNVSPISY 328
            +GL+G  + KLS   QT   F   FSYCL   +      S+  G    S     +P   
Sbjct: 269 AAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTP--- 328

Query: 329 TRMISDPEMPSFYLLNVTGISIGGVKL-----NTPRFASSGNGVTSLLDSGTTITRLPPS 388
             ++S+P++ +FY + + GIS+GG ++     +  +    GNG   ++DSGT++TRL   
Sbjct: 329 --LLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGV-IIDSGTSVTRLIRP 388

Query: 389 VYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLPSVKFYFEGDAVLTVDVQGML 448
            Y A +  F      L++ P +S+ + C++L+ + E ++P+V  +F G     V +    
Sbjct: 389 AYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRG---ADVSLPATN 448

Query: 449 YFLKSDYS-QTCLAFARLSGEDQLGIIGSYQQKNQRVLYNTQESKVGFAVETCS 489
           Y +  D + + C AFA   G   L IIG+ QQ+  RV+Y+   S+VGFA   C+
Sbjct: 449 YLIPVDTNGKFCFAFAGTMG--GLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of Lag0002842 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 9.3e-44
Identity = 124/384 (32.29%), Postives = 188/384 (48.96%), Query Frame = 0

Query: 125 SQIPISLGQPFQTLNYVVTVGIG--GQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDP 184
           S  P++ GQ     +YVV  G+G   Q L L +DT +D TW  C PC  C      +F P
Sbjct: 66  SSAPVASGQ--APPSYVVRAGLGSPSQQLLLALDTSADATWAHCSPCGTC--PSSSLFAP 125

Query: 185 SNSTSFLSLPCNSTTCSSLVYGHCAP---GTKNSSP-------CNYEISYGDGSYSKGDF 244
           +NS+S+ SLPC+S+ C  L  G   P   G  +++P       C +   + D S+ +   
Sbjct: 126 ANSSSYASLPCSSSWC-PLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADASF-QAAL 185

Query: 245 AYERLTLGMITVDKFIIGC--GQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYC 304
           A + L LG   +  +  GC    T         GL+G  R  ++++SQ  S++ G+FSYC
Sbjct: 186 ASDTLRLGKDAIPNYTFGCVSSVTGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYC 245

Query: 305 LPA-TGDGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIG--GVKL 364
           LP+      SGSL +G G          + YT M+ +P   S Y +NVTG+S+G   VK+
Sbjct: 246 LPSYRSYYFSGSLRLGAGG----GQPRSVRYTPMLRNPHRSSLYYVNVTGLSVGHAWVKV 305

Query: 365 NTPRFA-SSGNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNL 424
               FA  +  G  +++DSGT ITR    VY A + EF +Q             + C+N 
Sbjct: 306 PAGSFAFDAATGAGTVVDSGTVITRWTAPVYAALREEFRRQVAAPSGYTSLGAFDTCFNT 365

Query: 425 TEVKEFQLPSVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARL--SGEDQLGIIGSY 484
            EV     P+V  + +G   L + ++  L    S     CLA A    +    + +I + 
Sbjct: 366 DEVAAGGAPAVTVHMDGGVDLALPMENTLIH-SSATPLACLAMAEAPQNVNSVVNVIANL 425

Query: 485 QQKNQRVLYNTQESKVGFAVETCS 489
           QQ+N RV+++   S+VGFA E+C+
Sbjct: 426 QQQNIRVVFDVANSRVGFAKESCN 438

BLAST of Lag0002842 vs. ExPASy TrEMBL
Match: A0A6J1JS16 (aspartyl protease family protein At5g10770 OS=Cucurbita maxima OX=3661 GN=LOC111488391 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 2.5e-169
Identity = 309/494 (62.55%), Postives = 372/494 (75.30%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKKRAPTDCT 60
           MEI+KSL    LLLLLLLL F  ++RS       +    LL+L+ LPW Q ++ A  +C 
Sbjct: 1   MEISKSL-CFFLLLLLLLLFFVDEARSDAINGDSEKLHRLLHLQKLPWKQ-QEEAVVNCI 60

Query: 61  FQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISHNKPL 120
           FQKP+V  G TTL+MKERD CSGKV DW  NLQNR++ DAI +++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKERDYCSGKVTDWQNNLQNRLIFDAIRLQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G   QTLNY+VTV +GG++ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSLPCNSTTCSSLV-----YGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYE 240
           DPSNS+SFLSL CNS TC +L       G C  G  NSS C YEI+YGDGSYS+G+  +E
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPPATGNSGLC--GNGNSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG I +D FI GCG+ N+GL+G  SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPRFASS 360
            G SGSLTMGGGDFS++RNVSPISYTRM+S+P+MP+FY LN+TGI+IGGV L  P     
Sbjct: 301 AGASGSLTMGGGDFSNYRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGVP----- 360

Query: 361 GNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLP 420
            NG  SL+DSGT ITRL PS+Y AFKAEFEKQ    +  PG+SIL  C+NLT  KE  +P
Sbjct: 361 NNGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIP 420

Query: 421 SVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNT 480
           +VKF+FEG+A +TVDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKNQRV+YN+
Sbjct: 421 TVKFFFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNS 480

Query: 481 QESKVGFAVETCSF 490
           +ES VGFA E C F
Sbjct: 481 KESTVGFAAEPCGF 485

BLAST of Lag0002842 vs. ExPASy TrEMBL
Match: A0A6J1GQV9 (aspartyl protease family protein At5g10770 OS=Cucurbita moschata OX=3662 GN=LOC111456281 PE=3 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 1.3e-168
Identity = 308/494 (62.35%), Postives = 370/494 (74.90%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKKRAPTDCT 60
           MEI+KSL      LLLLLL F   +RS       +    LL+L+  PW Q ++ A  +C 
Sbjct: 1   MEISKSL---CFFLLLLLLFFVDQARSDAINGDSEKLHRLLHLQKRPWKQ-QEEAVINCI 60

Query: 61  FQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISHNKPL 120
           FQKP+V  G TTL+MKE+D CSG+V DW +NLQNR++ DAIHV++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKEKDYCSGEVTDWQKNLQNRLISDAIHVQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G   QTLNY+VTV +GG++ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSLPCNSTTCSSLV-----YGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYE 240
           DPSNS+SFLSL CNS TC +L       G C  G  NSS C YEI+YGDGSYS+G+  +E
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPPATGNSGLC--GNGNSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG I +D FI GCG+ N+GL+G  SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPRFASS 360
            G SGSLTMGGGDFS+FRNVSPISYTRM+S+P+MP+FY LN+TGI+IGGV L       S
Sbjct: 301 AGASGSLTMGGGDFSNFRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV-----S 360

Query: 361 GNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLP 420
            NG  SL+DSGT ITRL PS+Y AFKAEFEKQ    +  PG+SIL  C+NLT  KE  +P
Sbjct: 361 NNGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIP 420

Query: 421 SVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNT 480
           +VKFYFEG+A +TVDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKNQRV+YN+
Sbjct: 421 TVKFYFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNS 480

Query: 481 QESKVGFAVETCSF 490
           +ES VGFA E C F
Sbjct: 481 KESTVGFAAEPCGF 483

BLAST of Lag0002842 vs. ExPASy TrEMBL
Match: A0A0A0K8J2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G431320 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 3.1e-167
Identity = 307/501 (61.28%), Postives = 378/501 (75.45%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLL---LLLFAVDSRS----IGARNRHQNKKLLLNLRNLPWSQNKK 60
           MEI+KSLH P+ LLLLL   LL   VD+RS    +G  + H+ K LL   +N PW ++ +
Sbjct: 1   MEISKSLHFPLSLLLLLLLPLLSIGVDARSSSFNLGNGDNHE-KGLLQLFQNFPWKEHGE 60

Query: 61  RAPTDCTFQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSA 120
            A  +C FQKPK+  G TTL+MK+RD CSGK+ DW++  QNR++ DAI+V +L S  +SA
Sbjct: 61  -AVVNCIFQKPKITKGITTLEMKQRDYCSGKITDWEKIFQNRIILDAINVNSLFSHFKSA 120

Query: 121 ISHNKPLGISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCY 180
           I   +   +S+SQIPIS G   QTLNY+VTVGIGGQN TLIVDTGSD+TWVQC PCR CY
Sbjct: 121 IFPGQTHQLSDSQIPISSGARLQTLNYIVTVGIGGQNSTLIVDTGSDLTWVQCLPCRLCY 180

Query: 181 TQQEPIFDPSNSTSFLSLPCNSTTCSSL-----VYGHCAPGTKNSSPCNYEISYGDGSYS 240
            QQEP+F+PSNS+SFLSLPCNS TC +L       G C+   KNS+ C+Y+I YGDGSYS
Sbjct: 181 NQQEPLFNPSNSSSFLSLPCNSPTCVALQPTAGSSGLCS--NKNSTSCDYQIDYGDGSYS 240

Query: 241 KGDFAYERLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFS 300
           +G+  +E+LTLG   +D FI GCG+ N+GL+G  SGLMG  R +LS+VSQTSS+FG +FS
Sbjct: 241 RGELGFEKLTLGKTEIDNFIFGCGRNNKGLFGGASGLMGLARSELSLVSQTSSLFGSVFS 300

Query: 301 YCLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLN 360
           YCLP TG G+SGSLT+GG DFS+F+N+SPISYTRMI +P+M +FY LN+TGISIGGV LN
Sbjct: 301 YCLPTTGVGSSGSLTLGGADFSNFKNISPISYTRMIQNPQMSNFYFLNLTGISIGGVNLN 360

Query: 361 TPRFASSGNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTE 420
            PR  SS  GV SLLDSGT ITRL PS+Y AFKAEFEKQ    R  PG+SIL  C+NLT 
Sbjct: 361 VPRL-SSNEGVLSLLDSGTVITRLSPSIYKAFKAEFEKQFSGYRTTPGFSILNTCFNLTG 420

Query: 421 VKEFQLPSVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKN 480
            +E  +P+VKF FEG+A + VDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKN
Sbjct: 421 YEEVNIPTVKFIFEGNAEMIVDVEGVFYFVKSDASQICLAFASLGYEDQTMIIGNYQQKN 480

Query: 481 QRVLYNTQESKVGFAVETCSF 490
           QRV+YN++ESKVGFA E CSF
Sbjct: 481 QRVIYNSKESKVGFAGEPCSF 496

BLAST of Lag0002842 vs. ExPASy TrEMBL
Match: A0A1S3CDQ0 (aspartyl protease family protein At5g10770 OS=Cucumis melo OX=3656 GN=LOC103499859 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 4.0e-167
Identity = 307/498 (61.65%), Postives = 375/498 (75.30%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLL---LLLFAVDSR-SIGARNRHQNKKLLLNLRNLPWSQNKKRAP 60
           ME++KSLH P+ LL LL   LL   VD+R S+G    +  K LL   +N PW ++ + A 
Sbjct: 3   MEVSKSLHFPLSLLFLLLLPLLFIIVDARSSVGNGGNYHEKGLLQLFQNFPWKEHGE-AV 62

Query: 61  TDCTFQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISH 120
            +C FQKPK+  G TTL+MK+RD CSGK+ D ++  QNR++ DAI+V +L S V+SAI  
Sbjct: 63  VNCIFQKPKITKGITTLEMKQRDYCSGKITDLEKIFQNRIILDAINVNSLLSHVKSAIFP 122

Query: 121 NKPLGISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQ 180
            +   +S+SQIPIS G   QTLNY+VTVGIGGQN TLIVDTGSD+TWVQC PCR CY QQ
Sbjct: 123 GQTHQLSDSQIPISSGARLQTLNYIVTVGIGGQNSTLIVDTGSDLTWVQCLPCRLCYNQQ 182

Query: 181 EPIFDPSNSTSFLSLPCNSTTCSSL-----VYGHCAPGTKNSSPCNYEISYGDGSYSKGD 240
           EP+F+PSNS+SFLSLPC+S TC +L       G C+   KNS+ C+Y+I YGDGSYS+G+
Sbjct: 183 EPLFNPSNSSSFLSLPCSSPTCLALQPTAGSSGLCS--NKNSTSCDYQIDYGDGSYSRGE 242

Query: 241 FAYERLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCL 300
             YE+LTLG   +D FI GCG+ N+GL+G  SGLMG  R +LS+VSQTSSVFG IFSYCL
Sbjct: 243 LGYEKLTLGKTEIDNFIFGCGRNNKGLFGGASGLMGLARSELSLVSQTSSVFGSIFSYCL 302

Query: 301 PATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPR 360
           P TG G+SGSLT+GG DFSSF+N+SPISYTRMI +P+M +FY LN+TGISIGGV LN PR
Sbjct: 303 PTTGVGSSGSLTLGGTDFSSFKNISPISYTRMIQNPQMSNFYFLNLTGISIGGVNLNVPR 362

Query: 361 FASSGNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKE 420
             SS  GV SLLDSGT ITRL PS+Y AFKAEFEKQ    R  PG+SIL  C+NLT  +E
Sbjct: 363 L-SSNEGVLSLLDSGTVITRLSPSIYKAFKAEFEKQFSGYRTTPGFSILNTCFNLTGYEE 422

Query: 421 FQLPSVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRV 480
             +P+VKF FEG+A + VDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKNQRV
Sbjct: 423 VNIPTVKFIFEGNAEMIVDVEGVFYFVKSDASQICLAFASLGYEDQTMIIGNYQQKNQRV 482

Query: 481 LYNTQESKVGFAVETCSF 490
           +YN++ESKVGFA E CSF
Sbjct: 483 VYNSKESKVGFAGEPCSF 496

BLAST of Lag0002842 vs. ExPASy TrEMBL
Match: A0A5A7UYY6 (Aspartyl protease family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001610 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 1.2e-166
Identity = 309/499 (61.92%), Postives = 378/499 (75.75%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFA--VDSR--SIGARNRHQNKKLLLNL-RNLPWSQNKKRA 60
           ME++KSLH P+ LL LLL L +  VD+R  S G  N   ++K LL L +N PW ++ + A
Sbjct: 3   MEVSKSLHFPLSLLFLLLPLLSIIVDARSSSFGVGNGSNHEKGLLQLFQNFPWKEHGE-A 62

Query: 61  PTDCTFQKPKVENGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAIS 120
             +C FQKPK+  G TTL+MK+RD CSGK+ D ++  QNR++ DAI+V +L S V+SAI 
Sbjct: 63  VVNCIFQKPKITKGITTLEMKQRDYCSGKITDLEKIFQNRIILDAINVNSLLSHVKSAIF 122

Query: 121 HNKPLGISESQIPISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQ 180
             +   +S+SQIPIS G   QTLNY+VTVGIGGQN TLIVDTGSD+TWVQC PCR CY Q
Sbjct: 123 PGQTHQLSDSQIPISSGARLQTLNYIVTVGIGGQNSTLIVDTGSDLTWVQCLPCRLCYNQ 182

Query: 181 QEPIFDPSNSTSFLSLPCNSTTCSSL-----VYGHCAPGTKNSSPCNYEISYGDGSYSKG 240
           QEP+F+PSNS+SFLSLPC+S TC +L       G C+   KNS+ C+Y+I YGDGSYS+G
Sbjct: 183 QEPLFNPSNSSSFLSLPCSSPTCLALQPTAGSSGLCS--NKNSTSCDYQIDYGDGSYSRG 242

Query: 241 DFAYERLTLGMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYC 300
           +  YE+LTLG   +D FI GCG+ N+GL+G  SGLMG  R +LS+VSQTSSVFG IFSYC
Sbjct: 243 ELGYEKLTLGKTEIDNFIFGCGRNNKGLFGGASGLMGLARSELSLVSQTSSVFGSIFSYC 302

Query: 301 LPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTP 360
           LP TG G+SGSLT+GG DFSSF+N+SPISYTRMI +P+M +FY LN+TGISIGGV LN P
Sbjct: 303 LPTTGVGSSGSLTLGGTDFSSFKNISPISYTRMIQNPQMSNFYFLNLTGISIGGVNLNVP 362

Query: 361 RFASSGNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVK 420
           R  SS  GV SLLDSGT ITRL PS+Y AFKAEFEKQ    R  PG+SIL  C+NLT  +
Sbjct: 363 RL-SSNEGVLSLLDSGTVITRLSPSIYKAFKAEFEKQFSGYRTTPGFSILNTCFNLTGYE 422

Query: 421 EFQLPSVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQR 480
           E  +P+VKF FEG+A + VDV+G+ YF+KSD SQ CLAFA L  EDQ  IIG+YQQKNQR
Sbjct: 423 EVNIPTVKFIFEGNAEMIVDVEGVFYFVKSDASQICLAFASLGYEDQTMIIGNYQQKNQR 482

Query: 481 VLYNTQESKVGFAVETCSF 490
           V+YN++ESKVGFA E CSF
Sbjct: 483 VVYNSKESKVGFAGEPCSF 497

BLAST of Lag0002842 vs. TAIR 10
Match: AT1G79720.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 438.3 bits (1126), Expect = 7.9e-123
Identity = 227/488 (46.52%), Postives = 321/488 (65.78%), Query Frame = 0

Query: 9   SPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKK-RAPTDCTFQKPKVE 68
           +P+LL+ L LL   V            ++K +L++ N  WS  K   A T C  +     
Sbjct: 12  APLLLVFLFLLSCVVHG---------VDEKKILSVHNNIWSPKKSYEASTSCFSRSLGKG 71

Query: 69  NGATTLQMKERDDCSGKVIDWDENLQNRVLRDAIHVKALQSRVRSAISHNKPLGISESQI 128
             +TTL+MK R+ CSGK ID  + ++  ++ D I V++LQ ++++  S      +SE+QI
Sbjct: 72  RESTTLEMKHRELCSGKTIDLGKKMRRALVLDNIRVQSLQLKIKAMTSSTTEQSVSETQI 131

Query: 129 PISLGQPFQTLNYVVTVGIGGQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTS 188
           P++ G   ++LNY+VTV +GG+N++LIVDTGSD+TWVQC+PCR CY QQ P++DPS S+S
Sbjct: 132 PLTSGIKLESLNYIVTVELGGKNMSLIVDTGSDLTWVQCQPCRSCYNQQGPLYDPSVSSS 191

Query: 189 FLSLPCNSTTCSSLV-----YGHCAPGTKN---SSPCNYEISYGDGSYSKGDFAYERLTL 248
           + ++ CNS+TC  LV      G C  G  N    +PC Y +SYGDGSY++GD A E + L
Sbjct: 192 YKTVFCNSSTCQDLVAATSNSGPC--GGNNGVVKTPCEYVVSYGDGSYTRGDLASESILL 251

Query: 249 GMITVDKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDGTS 308
           G   ++ F+ GCG+ N+GL+G +SGLMG  R  +S+VSQT   F G+FSYCLP+  DG S
Sbjct: 252 GDTKLENFVFGCGRNNKGLFGGSSGLMGLGRSSVSLVSQTLKTFNGVFSYCLPSLEDGAS 311

Query: 309 GSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTPRFASSGNGV 368
           GSL+  G D S + N + +SYT ++ +P++ SFY+LN+TG SIGGV+L +  F   G G+
Sbjct: 312 GSLSF-GNDSSVYTNSTSVSYTPLVQNPQLRSFYILNLTGASIGGVELKSSSF---GRGI 371

Query: 369 TSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLPSVKF 428
             L+DSGT ITRLPPS+Y A K EF KQ       PGYSIL+ C+NLT  ++  +P +K 
Sbjct: 372 --LIDSGTVITRLPPSIYKAVKIEFLKQFSGFPTAPGYSILDTCFNLTSYEDISIPIIKM 431

Query: 429 YFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNTQESK 488
            F+G+A L VDV G+ YF+K D S  CLA A LS E+++GIIG+YQQKNQRV+Y+T + +
Sbjct: 432 IFQGNAELEVDVTGVFYFVKPDASLVCLALASLSYENEVGIIGNYQQKNQRVIYDTTQER 482

BLAST of Lag0002842 vs. TAIR 10
Match: AT5G10770.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 272.3 bits (695), Expect = 7.5e-73
Identity = 185/498 (37.15%), Postives = 264/498 (53.01%), Query Frame = 0

Query: 1   MEIAKSLHSPILLLLLLLLLFAVDSRSIGARNRHQNKKLLLNLRNLPWSQNKKRAPTDCT 60
           M I ++L + I++L + L L   D    GA+ R  +   +     LP S       + C 
Sbjct: 1   MSINRNLLNIIIILCICLNLGCND----GAQERETDSHTIQVSSLLPSSS------SSCV 60

Query: 61  FQKPKVENGATTLQMKER-DDCS----GKVIDWDENLQNRVLR-DAIHVKALQSRVRSAI 120
              P+     ++L +  R   CS    GK    D      +LR D   V ++ S++   +
Sbjct: 61  L-SPRASTTKSSLHVTHRHGTCSRLNNGKATSPD---HVEILRLDQARVNSIHSKLSKKL 120

Query: 121 SHNKPLGISESQIPISLGQPFQTLNYVVTVGIG--GQNLTLIVDTGSDITWVQCRPC-RK 180
           + +       + +P   G    + NY+VTVG+G    +L+LI DTGSD+TW QC+PC R 
Sbjct: 121 ATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSLIFDTGSDLTWTQCQPCVRT 180

Query: 181 CYTQQEPIFDPSNSTSFLSLPCNSTTCSSLVYGHCAPGTKNSSPCNYEISYGDGSYSKGD 240
           CY Q+EPIF+PS STS+ ++ C+S  C SL       G+ ++S C Y I YGD S+S G 
Sbjct: 181 CYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCSASNCIYGIQYGDQSFSVGF 240

Query: 241 FAYERLTLGMITV-DKFIIGCGQTNEGLYGETSGLMGFDRDKLSIVSQTSSVFGGIFSYC 300
            A E+ TL    V D    GCG+ N+GL+   +GL+G  RDKLS  SQT++ +  IFSYC
Sbjct: 241 LAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRDKLSFPSQTATAYNKIFSYC 300

Query: 301 LPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISDPEMPSFYLLNVTGISIGGVKLNTP 360
           LP++   T G LT G    S     +PIS    I+D    SFY LN+  I++GG KL  P
Sbjct: 301 LPSSASYT-GHLTFGSAGISRSVKFTPIS---TITDG--TSFYGLNIVAITVGGQKLPIP 360

Query: 361 RFASSGNGVTSLLDSGTTITRLPPSVYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVK 420
               S  G  +L+DSGT ITRLPP  Y A ++ F+ +  +     G SIL+ C++L+  K
Sbjct: 361 STVFSTPG--ALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTSGVSILDTCFDLSGFK 420

Query: 421 EFQLPSVKFYFEGDAVLTVDVQGMLYFLKSDYSQTCLAFARLSGEDQLGIIGSYQQKNQR 480
              +P V F F G AV+ +  +G+ Y  K   SQ CLAFA  S +    I G+ QQ+   
Sbjct: 421 TVTIPKVAFSFSGGAVVELGSKGIFYVFK--ISQVCLAFAGNSDDSNAAIFGNVQQQTLE 474

Query: 481 VLYNTQESKVGFAVETCS 489
           V+Y+    +VGFA   CS
Sbjct: 481 VVYDGAGGRVGFAPNGCS 474

BLAST of Lag0002842 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-63
Identity = 136/406 (33.50%), Postives = 211/406 (51.97%), Query Frame = 0

Query: 92  LQNRVLRDAIHVKALQSRVRSAI--SHNKPLGISESQIPISLGQPFQTLNYVVTVGIGG- 151
           L  R+ RD   V A+  R+   +  S +    +++    I  G    +  Y V +G+G  
Sbjct: 81  LHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSP 140

Query: 152 -QNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTSFLSLPCNSTTCSSLVYGHCA 211
            ++  +++D+GSD+ WVQC+PC+ CY Q +P+FDP+ S S+  + C S+ C  +    C 
Sbjct: 141 PRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGC- 200

Query: 212 PGTKNSSPCNYEISYGDGSYSKGDFAYERLTLGMITVDKFIIGCGQTNEGLYGETSGLMG 271
               +S  C YE+ YGDGSY+KG  A E LT     V    +GCG  N G++   +GL+G
Sbjct: 201 ----HSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLG 260

Query: 272 FDRDKLSIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPI--SYTRMIS 331
                +S V Q S   GG F YCL + G  ++GSL  G       R   P+  S+  ++ 
Sbjct: 261 IGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFG-------REALPVGASWVPLVR 320

Query: 332 DPEMPSFYLLNVTGISIGGVKLNTP----RFASSGNGVTSLLDSGTTITRLPPSVYDAFK 391
           +P  PSFY + + G+ +GGV++  P        +G+G   ++D+GT +TRLP + Y AF+
Sbjct: 321 NPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV-VMDTGTAVTRLPTAAYVAFR 380

Query: 392 AEFEKQCPELRKVPGYSILEACYNLTEVKEFQLPSVKFYFEGDAVLTVDVQGMLYFLKSD 451
             F+ Q   L +  G SI + CY+L+     ++P+V FYF    VLT+  +  L  +  D
Sbjct: 381 DGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPV-DD 440

Query: 452 YSQTCLAFARLSGEDQLGIIGSYQQKNQRVLYNTQESKVGFAVETC 488
               C AFA  +    L IIG+ QQ+  +V ++     VGF    C
Sbjct: 441 SGTYCFAFA--ASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Lag0002842 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 236.5 bits (602), Expect = 4.5e-62
Identity = 146/411 (35.52%), Postives = 223/411 (54.26%), Query Frame = 0

Query: 95  RVLRDAIHVKALQSRVRSAISH-----NKPLGI------SESQIPISLGQPFQTLNYVVT 154
           R+ RD   VK+L +R+  AI++      KP+         + + P+  G    +  Y   
Sbjct: 92  RLNRDTARVKSLITRLDLAINNISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTR 151

Query: 155 VGIG--GQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTSFLSLPCNSTTCSSL 214
           VGIG   + + +++DTGSD+ W+QC PC  CY Q EPIF+PS+S+S+  L C++  C++L
Sbjct: 152 VGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNAL 211

Query: 215 VYGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYERLTLGMITVDKFIIGCGQTNEGLYGE 274
               C   T     C YE+SYGDGSY+ GDFA E LT+G   V    +GCG +NEGL+  
Sbjct: 212 EVSECRNAT-----CLYEVSYGDGSYTVGDFATETLTIGSTLVQNVAVGCGHSNEGLFVG 271

Query: 275 TSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPISYT 334
            +GL+G     L++ SQ ++     FSYCL    D  S S    G   S    V+P    
Sbjct: 272 AAGLLGLGGGLLALPSQLNTT---SFSYCL-VDRDSDSASTVDFGTSLSPDAVVAP---- 331

Query: 335 RMISDPEMPSFYLLNVTGISIGGVKLNTPRFA----SSGNGVTSLLDSGTTITRLPPSVY 394
            ++ + ++ +FY L +TGIS+GG  L  P+ +     SG+G   ++DSGT +TRL   +Y
Sbjct: 332 -LLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSG-GIIIDSGTAVTRLQTEIY 391

Query: 395 DAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLPSVKFYFEGDAVLTVDVQGMLYF 454
           ++ +  F K   +L K  G ++ + CYNL+     ++P+V F+F G  +L +  +   Y 
Sbjct: 392 NSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKN--YM 451

Query: 455 LKSDYSQT-CLAFARLSGEDQLGIIGSYQQKNQRVLYNTQESKVGFAVETC 488
           +  D   T CLAFA  +    L IIG+ QQ+  RV ++   S +GF+   C
Sbjct: 452 IPVDSVGTFCLAFAPTA--SSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

BLAST of Lag0002842 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 229.6 bits (584), Expect = 5.5e-60
Identity = 139/414 (33.57%), Postives = 218/414 (52.66%), Query Frame = 0

Query: 89  DENLQNRVLRDAIHVKALQSRVRSAISHN-----KPLGISESQIPISLGQPFQTLNYVVT 148
           DE   +R+ RD+  VK++ +        N     +P G S S +    G    +  Y   
Sbjct: 89  DELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVS---GLSQGSGEYFTR 148

Query: 149 VGIG--GQNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTSFLSLPCNSTTCSSL 208
           +G+G   + + +++DTGSDI W+QC PCR+CY+Q +PIFDP  S ++ ++PC+S  C  L
Sbjct: 149 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRL 208

Query: 209 VYGHCAPGTKNSSPCNYEISYGDGSYSKGDFAYERLTLGMITVDKFIIGCGQTNEGLYGE 268
               C    K    C Y++SYGDGS++ GDF+ E LT     V    +GCG  NEGL+  
Sbjct: 209 DSAGCNTRRKT---CLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVG 268

Query: 269 TSGLMGFDRDKLSIVSQTSSVFGGIFSYCL-PATGDGTSGSLTMGGGDFSSFRNVSPISY 328
            +GL+G  + KLS   QT   F   FSYCL   +      S+  G    S     +P   
Sbjct: 269 AAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTP--- 328

Query: 329 TRMISDPEMPSFYLLNVTGISIGGVKL-----NTPRFASSGNGVTSLLDSGTTITRLPPS 388
             ++S+P++ +FY + + GIS+GG ++     +  +    GNG   ++DSGT++TRL   
Sbjct: 329 --LLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGV-IIDSGTSVTRLIRP 388

Query: 389 VYDAFKAEFEKQCPELRKVPGYSILEACYNLTEVKEFQLPSVKFYFEGDAVLTVDVQGML 448
            Y A +  F      L++ P +S+ + C++L+ + E ++P+V  +F G     V +    
Sbjct: 389 AYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRG---ADVSLPATN 448

Query: 449 YFLKSDYS-QTCLAFARLSGEDQLGIIGSYQQKNQRVLYNTQESKVGFAVETCS 489
           Y +  D + + C AFA   G   L IIG+ QQ+  RV+Y+   S+VGFA   C+
Sbjct: 449 YLIPVDTNGKFCFAFAGTMG--GLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6575642.13.0e-17264.04Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
KAG7014194.18.6e-17263.36Aspartyl protease family protein [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023547381.12.1e-17062.96aspartyl protease family protein At5g10770 [Cucurbita pepo subsp. pepo][more]
XP_022991886.15.2e-16962.55aspartyl protease family protein At5g10770 [Cucurbita maxima][more]
XP_022953875.12.6e-16862.35aspartyl protease family protein At5g10770 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8S9J61.1e-7137.15Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9LHE32.6e-6233.50Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LEW37.8e-5934.07Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Q9LNJ37.8e-5933.57Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q6F4N59.3e-4432.29Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JS162.5e-16962.55aspartyl protease family protein At5g10770 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GQV91.3e-16862.35aspartyl protease family protein At5g10770 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A0A0K8J23.1e-16761.28Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G43132... [more]
A0A1S3CDQ04.0e-16761.65aspartyl protease family protein At5g10770 OS=Cucumis melo OX=3656 GN=LOC1034998... [more]
A0A5A7UYY61.2e-16661.92Aspartyl protease family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT1G79720.17.9e-12346.52Eukaryotic aspartyl protease family protein [more]
AT5G10770.17.5e-7337.15Eukaryotic aspartyl protease family protein [more]
AT3G20015.11.8e-6333.50Eukaryotic aspartyl protease family protein [more]
AT1G25510.14.5e-6235.52Eukaryotic aspartyl protease family protein [more]
AT1G01300.15.5e-6033.57Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 361..372
score: 34.45
coord: 144..164
score: 44.28
coord: 300..313
score: 32.03
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 13..488
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 118..305
e-value: 2.0E-46
score: 160.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 311..488
e-value: 9.2E-42
score: 144.9
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 133..487
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 333..483
e-value: 6.7E-28
score: 97.5
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 140..306
e-value: 2.3E-46
score: 158.2
NoneNo IPR availablePANTHERPTHR13683:SF827SUBFAMILY NOT NAMEDcoord: 13..488
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 153..164
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 140..483
score: 40.521667

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002842.1Lag0002842.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity