Lag0002830 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002830 vs. NCBI nr
Match: XP_022953208.1 (4-coumarate--CoA ligase 2-like [Cucurbita moschata])

HSP 1 Score: 966.1 bits (2496), Expect = 1.3e-277
Identity = 487/542 (89.85%), Postives = 513/542 (94.65%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN AT  VYSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKK+DV+MNLLPNSPEFVFTFLAASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAANAKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P VE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY   DV+LCVLP FH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEI+SLL+L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSL FAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANS+GEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGFVDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG PDE AGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA+G AI EED+KQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lag0002830 vs. NCBI nr
Match: XP_023547628.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.5 bits (2492), Expect = 3.8e-277
Identity = 487/542 (89.85%), Postives = 512/542 (94.46%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN AT  V+SY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVHSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKK DV+MNLLPNSPEFVFTFLAASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAANAKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P VE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL+L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSL FAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANS+GEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG PDE AGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA+G AI EED+KQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lag0002830 vs. NCBI nr
Match: XP_022991872.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 964.5 bits (2492), Expect = 3.8e-277
Identity = 486/542 (89.67%), Postives = 511/542 (94.28%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN AT  +YSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKK DV+MNLLPNSPEFVFTFL ASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAANAKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH PPVE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL+L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSL FAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANS+GEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG PDE AGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA+G AI EED+KQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lag0002830 vs. NCBI nr
Match: KAG6593348.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 962.2 bits (2486), Expect = 1.9e-276
Identity = 487/542 (89.85%), Postives = 506/542 (93.36%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQN +KFGSRPCLIN AT  VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LT+RRVASGLH LGIKK DV+MNLLPNSPEFVFTFL ASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAAN KLIVTMACFYD+VKDLAE+GVKIVCVD AVE CLH+SVLSGADESH P V+  A
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVS LQLVEKYKVSIM IVPPI LAIAKSPE EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPELEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELE+AVR KFP+A+LGQGYGMTEAGPVLTMSL FAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIG PDE AGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA+G AI EED+KQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of Lag0002830 vs. NCBI nr
Match: XP_022959958.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 959.1 bits (2478), Expect = 1.6e-275
Identity = 485/542 (89.48%), Postives = 506/542 (93.36%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQN +KFGSRPCLIN AT  VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LT+RRVASGLH LGIKK DV+MNLLPNSPEFVFTFL ASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAAN KLIVTMACFYD+VKDLAE+GVKIVCVD AVE CLH+SVLSGADESH P V+  A
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVS LQLVEKYKVSIM IVPPI LAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELE+AVR KFP+A+LGQGYGMTEAGPVLTMSL FAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGF+D+DDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIG PDE AGEVPV
Sbjct: 421 IGFIDNDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA+G AI EED+KQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of Lag0002830 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 9.1e-226
Identity = 391/543 (72.01%), Postives = 455/543 (83.79%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           M  +T Q  + IFRSKLPDI+IPNHLPLH Y F+N ++F SRPCLIN A   +Y+Y DV+
Sbjct: 1   MEKDTKQV-DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVE 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SR+VA+GLH  GI+  D IM LLPNSPEFVF F+ ASY GAI T ANP  T  E+ KQ
Sbjct: 61  LNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLA-EDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEID 180
           AKA++AK+IVT AC  +KVKD A E+ VKI+C+D A EGCLHFSVL+ A+E   P VEI 
Sbjct: 121 AKASSAKIIVTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQ 180

Query: 181 AEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY   +DV+LCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIY 240

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDV 300
           SLNS+LLCGLR GAAILIMQKF+IVS L+L+++YKV+I   VPPI+LAIAKSP  + YD+
Sbjct: 241 SLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDL 300

Query: 301 SSVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGA 360
           SSVR + SG APLGKELE+ VRAKFP+A LGQGYGMTEAGPVL M L FAKEPF +K+GA
Sbjct: 301 SSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGA 360

Query: 361 CGTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N SGEICIRGDQIMKGYLND E+T RTIDKEGWL+TG
Sbjct: 361 CGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTG 420

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVP 480
           DIG++DDDDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+   DE AGEVP
Sbjct: 421 DIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 480

Query: 481 VAFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VAFVV+++G  I E+++K FISKQV+FYKR+ RVFFV+ IPK+PSGKILRK+LRAKLA+G
Sbjct: 481 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 LPN 542

BLAST of Lag0002830 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 7.7e-225
Identity = 384/543 (70.72%), Postives = 454/543 (83.61%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           M  ETNQ  + IFRSKLPDI+IP HLPLH Y F+N ++F SRPCLIN A  H+Y+Y DV+
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LTSR+VA+GL+ LGI++ D IM LLPNSPEFVF F+ ASY GAI T ANP  T  E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDKVKDLA-EDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEID 180
           AKA+NAKLI+T ACF +KVKD A ++ + ++C+D A EGC+HFS L+ ADE   P V+I 
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 AEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIY 240
           ++DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG N NLY   +DV++CVLPLFHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDV 300
           SLNS+LLCGLR GAAILIMQKF+IV   +L+EKYKV+I   VPPI+LAIAKSP  + YD+
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGA 360
           SSVR + SG APLGKELE+AVR KFP+A LGQGYGMTEAGPVL M L FAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND  +T RTIDKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVP 480
           DIG++D+DDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+   DE AGEVP
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VAFVV+++G  I E+++K F+SKQV+FYKR+ RVFFV T+PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 VPN 544

BLAST of Lag0002830 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 3.8e-224
Identity = 383/540 (70.93%), Postives = 451/540 (83.52%), Query Frame = 0

Query: 4   ETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQLTS 63
           ET Q+ + IFRSKLPDI+IP HLPLH Y F+N ++F SRPCLIN A   +Y+Y +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKA 123
           R+VA GL+ LGI++ D IM LLPNSPEFVF F+ ASY GAI T ANP  T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDKVKDLA-EDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDAED 183
           ++AK+I+T +CF  KVKD A E+ VK++C+D A EGCLHFS L+ +DE   P V+I  +D
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG N NLY   +DV++CVLPLFHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVSSV 303
           SILLCGLR GAAILIMQKF+I   L+L++KYKVSI   VPPI+LAIAKSP  + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGACGT 363
           R + SG APLGKELE+AVR KFP+A LGQGYGMTEAGPVL M L FAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPVAF 483
           F+D+DDELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+   DE AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGAYN 543
           VV+++G AI E+++K FISKQV+FYKR+ RVFFV T+PK+PSGKILRK+LRA+LA+G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of Lag0002830 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 8.5e-224
Identity = 395/546 (72.34%), Postives = 454/546 (83.15%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           +A+E  Q  + IFRSKLPDI+IP +LPLH Y F+N +KF SRPCLIN AT  +++Y DV+
Sbjct: 5   VAIE-EQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVE 64

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRV SGL  LGIK+ D IM LLPNSPEFVF FL AS+ G+I T ANPF T+ E+ KQ
Sbjct: 65  LISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 124

Query: 121 AKAANAKLIVTMACFYDKVKDLA-EDGVKIVCVDLA-----VEGCLHFSVLSGADESHAP 180
           AKA+NAKLI+T  C+ DKVKD A E+GVKI+ +D           LHFS L+GADE+  P
Sbjct: 125 AKASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMP 184

Query: 181 PVEIDAEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLP 240
            VEI  + VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY   DDV+LCVLP
Sbjct: 185 KVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLP 244

Query: 241 LFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEF 300
           LFHIYSLNS+LLCGLRAG+ ILIMQKFEIV  L+L++KYKV+I   VPPI+LAIAKS   
Sbjct: 245 LFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVV 304

Query: 301 EKYDVSSVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFP 360
           + YD+SSVR + SG APLGKELE+AVRAKFP+A LGQGYGMTEAGPVL M L FAKEPF 
Sbjct: 305 DNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 364

Query: 361 VKAGACGTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEG 420
           +K+GACGTVVRNAEMKIVDPETG+SLP N  GEICIRGDQIMKGYLND E+T RTIDKEG
Sbjct: 365 IKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEG 424

Query: 421 WLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEH 480
           WLHTGDIG++DDDDELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+   DE 
Sbjct: 425 WLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEA 484

Query: 481 AGEVPVAFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRA 540
           AGEVPVAFVVK++G  I E++IKQFISKQV+FYKR+NRVFFV  IPKAPSGKILRK+LRA
Sbjct: 485 AGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRA 544

BLAST of Lag0002830 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 6.1e-222
Identity = 381/543 (70.17%), Postives = 449/543 (82.69%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           M +ET Q+ + IFRSKLPDI+IP HLPLH Y F+N ++F SRPCLI+ A   +Y+Y +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LTSR+VA GL+ LGI++ D IM LLPN PEFVF F+ ASY GAI T ANP  T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDKVKDLA-EDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEID 180
           AKA++AK+++T ACF  KVKD A E+ +K++CVD A EGC+HFS L  +DE   P V+I 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 AEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG N NLY   DDV++CVLPLFHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDV 300
           SLNS+LLC LR GAAILIMQKF+I   L+L+ K+KV+I   VPPI+LAIAKSP    YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGA 360
           SSVR + SG APLGKELE+AVRAKFP+A LGQGYGMTEAGPVL M L FAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVP 480
           DIGF+DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+   DE AGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VAFVV+++G  I E+++K FISKQV+FYKR+ RVFFV T+PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of Lag0002830 vs. ExPASy TrEMBL
Match: A0A6J1GMS9 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455820 PE=4 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 6.2e-278
Identity = 487/542 (89.85%), Postives = 513/542 (94.65%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN AT  VYSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKK+DV+MNLLPNSPEFVFTFLAASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAANAKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P VE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY   DV+LCVLP FH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEI+SLL+L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSL FAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANS+GEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGFVDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG PDE AGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA+G AI EED+KQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lag0002830 vs. ExPASy TrEMBL
Match: A0A6J1JN39 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488383 PE=4 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.8e-277
Identity = 486/542 (89.67%), Postives = 511/542 (94.28%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN AT  +YSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKK DV+MNLLPNSPEFVFTFL ASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAANAKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH PPVE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL+L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSL FAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANS+GEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG PDE AGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA+G AI EED+KQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lag0002830 vs. ExPASy TrEMBL
Match: A0A6J1H7E7 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460855 PE=4 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 7.6e-276
Identity = 485/542 (89.48%), Postives = 506/542 (93.36%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQN +KFGSRPCLIN AT  VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LT+RRVASGLH LGIKK DV+MNLLPNSPEFVFTFL ASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAAN KLIVTMACFYD+VKDLAE+GVKIVCVD AVE CLH+SVLSGADESH P V+  A
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVS LQLVEKYKVSIM IVPPI LAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELE+AVR KFP+A+LGQGYGMTEAGPVLTMSL FAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGF+D+DDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIG PDE AGEVPV
Sbjct: 421 IGFIDNDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA+G AI EED+KQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of Lag0002830 vs. ExPASy TrEMBL
Match: A0A6J1KT15 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497966 PE=4 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 6.5e-275
Identity = 483/542 (89.11%), Postives = 505/542 (93.17%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQN +K GSRPCLIN AT  VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LT+RRVASGLH LGIKK +V+MNLLPNSPEFVFTFL ASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAAN KLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P V+  A
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIM IVPPI LAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELE+AVR KFP+A+LGQGYGMTEAGPVLTMSL FAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGFVDD++ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIG PDE AGEVPV
Sbjct: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA+G AI EED+ QFIS+QVVFYKRL RVFFVN IPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Lag0002830 vs. ExPASy TrEMBL
Match: A0A6J1KVH9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497965 PE=4 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.7e-273
Identity = 476/542 (87.82%), Postives = 504/542 (92.99%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQ 60
           MA E NQT EFIFRSKLPDIHIPNHLPLHEY+FQN   +GSRPCLIN AT HVYSYDDVQ
Sbjct: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60

Query: 61  LTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LT+RRVASGLH LGIKK DV+MNLLPNSPEFVFTFL ASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAANAKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P V+  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYY R+DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL+LVEKYK SI+ IVPPILLAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300

Query: 301 SVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGK+LE+AVR KFP+A+LGQGYGMTEAGPVLTMSL FAKEPFPVK GAC
Sbjct: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+DPETG SLPANS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIG PDE AGE+PV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480

Query: 481 AFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVV A+G  I EED+KQFIS+QVVFYKRL RVFFVN IPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Lag0002830 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 758.1 bits (1956), Expect = 5.0e-219
Identity = 373/539 (69.20%), Postives = 456/539 (84.60%), Query Frame = 0

Query: 8   TNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQLTSRRVA 67
           +N+ IFRS+LPDI+IPNHLPLH+Y+F+N ++F ++PCLIN  T  VY+Y DV +TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  SGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKAANAK 127
           +GLH LG+K+ DV+M LLPNSPE V TFLAAS+ GAI T+ANPF T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDKVKDLAEDGVKIVCV--DLAVEGCLHFSVLSGADESHAP--PVEIDAEDV 187
           LIVT + + DK+K+L  DGV IV    D   E CL FS L+ ++E      P +I  EDV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY++RDDV+LCVLP+FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVSSVR 307
           I+LC LR GA ILIM KFEI  LL+ +++ KV++ ++VPPI+LAIAKSPE EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGACGTV 367
           ++KSG APLGKELE+A+ AKFP+A LGQGYGMTEAGPVL MSLGFAKEPFPVK+GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGF 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  TIDK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 VDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPVAFV 487
           +DDDDELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+   +E AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGAYN 543
           V++    I+E++IKQF+SKQVVFYKR+N+VFF ++IPKAPSGKILRK+LRA+LA+G  N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of Lag0002830 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 747.7 bits (1929), Expect = 6.7e-216
Identity = 375/543 (69.06%), Postives = 447/543 (82.32%), Query Frame = 0

Query: 6   NQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQLTSRR 65
           N  ++ IFRSKLPDI+IPNHL LH+Y+FQN ++F ++PCLIN  T HVY+Y DV + SR+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKAAN 125
           +A+  H LG+ + DV+M LLPN PEFV +FLAAS+RGA  TAANPF T  EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 AKLIVTMACFYDKVKDLA-EDGVKIVCVD-----LAVEGCLHFSVLSGADESHA---PPV 185
            KLI+T A + DK+K L  +DGV IVC+D        EGCL F+ L+ +    +     V
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 EIDAEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLF 245
           EI  +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY+  DDV+LCVLP+F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LL+L+++ KV++  +VPPI+LAIAKS E EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDVSSVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVK 365
           YD+SS+RV+KSG APLGKELE+AV AKFP+A LGQGYGMTEAGPVL MSLGFAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGACGTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWL 425
           +GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAG 485
           HTGDIG +DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+   +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 EVPVAFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKL 540
           EVPVAFVVK+    ++E+D+KQF+SKQVVFYKR+N+VFF  +IPKAPSGKILRK+LRAKL
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of Lag0002830 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 690.3 bits (1780), Expect = 1.3e-198
Identity = 347/508 (68.31%), Postives = 414/508 (81.50%), Query Frame = 0

Query: 6   NQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQLTSRR 65
           N  ++ IFRSKLPDI+IPNHL LH+Y+FQN ++F ++PCLIN  T HVY+Y DV + SR+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKAAN 125
           +A+  H LG+ + DV+M LLPN PEFV +FLAAS+RGA  TAANPF T  EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 AKLIVTMACFYDKVKDLA-EDGVKIVCVD-----LAVEGCLHFSVLSGADESHA---PPV 185
            KLI+T A + DK+K L  +DGV IVC+D        EGCL F+ L+ +    +     V
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 EIDAEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLF 245
           EI  +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY+  DDV+LCVLP+F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LL+L+++ KV++  +VPPI+LAIAKS E EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDVSSVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVK 365
           YD+SS+RV+KSG APLGKELE+AV AKFP+A LGQGYGMTEAGPVL MSLGFAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGACGTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWL 425
           +GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAG 485
           HTGDIG +DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+   +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 EVPVAFVVKADGDAIAEEDIKQFISKQV 505
           EVPVAFVVK+    ++E+D+KQF+SKQV
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Lag0002830 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 669.1 bits (1725), Expect = 3.0e-192
Identity = 329/546 (60.26%), Postives = 422/546 (77.29%), Query Frame = 0

Query: 4   ETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFG----SRPCLINAATAHVYSYDDV 63
           E   +++FIFRSKLPDI IPNHLPL +YVFQ  +  G    S  C+I+ AT  + +Y DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTSRRVASGLHTLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAK 123
           Q   RR+A+G+H LGI+  DV+M LLPNSPEF  +FLA +Y GA+ T ANPF T  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDKVKDLAEDGVKIVCVD---------LAVEGCLHFSVLSGADE 183
           QAKA+ AK+I+T  C  DK+ +L  DGV IVC+D          + +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SHAPPVEIDAEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVL 243
           +     +I  ED VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG NPNL +  +DV+L
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPLFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAK 303
           C LP+FHIY+L++++L  +R GAA+LI+ +FE+  +++L+++YKV+++ + PP++LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPEFEKYDVSSVRVLKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAK 363
           SPE E+YD+SSVR++ SG A L KELE+AVR KFP+A+ GQGYGMTE+G V   SL FAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFPVKAGACGTVVRNAEMKIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTI 423
            PF  K+GACGTV+RNAEMK+VD ETG SLP N SGEIC+RG Q+MKGYLND E+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGK 483
           DK+GWLHTGDIGFVDDDDE+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+  
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDEHAGEVPVAFVVKADGDAIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRK 537
            DE A EVPVAFV ++ G  + E+D+K +++KQVV YKR+  VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of Lag0002830 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 657.1 bits (1694), Expect = 1.2e-188
Identity = 329/527 (62.43%), Postives = 416/527 (78.94%), Query Frame = 0

Query: 12  IFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATAHVYSYDDVQLTSRRVASGLH 71
           IFRSKLPDI IPNHLPLH Y F+  +    +PCLI  +T   Y+Y +  L  RRVASGL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  TLGIKKADVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKAANAKLIVT 131
            LGI+K DVIM LL NS EFVF+F+ AS  GA+ T ANPF T+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESH--APPVEIDAEDVVALPYS 191
            + + DK+K+L E+   I   +   E CL FS L   DE++     V+I  +D  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYDRDDVVLCVLPLFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY   +DV+LCVLPLFHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RAGAAILIMQKFEIVSLLQLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVSSVRVLKSGG 311
           R+GA +L+M KFEI +LL L+++++V+I  +VPP+++A+AK+P    YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLGFAKEPFPVKAGACGTVVRNAEM 371
           APLGKEL++++R + P A+LGQGYGMTEAGPVL+MSLGFAKEP P K+G+CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDPETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDE 431
           K+V  ET  SL  N  GEICIRG QIMK YLND E+T  TID+EGWLHTGDIG+VD+DDE
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKPDEHAGEVPVAFVVKADGD 491
           +FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+ + DE AGEVPVAFVV+++G+
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 AIAEEDIKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKL 537
            I EED+K++++KQVVFYKRL++VFFV +IPK+PSGKILRK+L+AKL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560