Lag0002771 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002771 vs. NCBI nr
Match: XP_038899303.1 (polygalacturonase QRT2-like [Benincasa hispida])

HSP 1 Score: 794.7 bits (2051), Expect = 4.3e-226
Identity = 395/448 (88.17%), Postives = 415/448 (92.63%), Query Frame = 0

Query: 12  LLLLLCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLH-YEYSSEFSSMLKTKL 71
           LLLL  L+ + +  FFS C GSYADI D P SISSGD +DHD H YEYSSEFSSML+T+L
Sbjct: 13  LLLLSFLITMAISEFFSTCSGSYADI-DPPPSISSGDKEDHDQHYYEYSSEFSSMLRTRL 72

Query: 72  EKMAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDRTYHLKP 131
           EKMAPSPV+S +EIFNVDDYGA+GDGEDDSEAFKEAWK+ACSSTN IFLVP DR YHLKP
Sbjct: 73  EKMAPSPVAS-SEIFNVDDYGAMGDGEDDSEAFKEAWKDACSSTNAIFLVPCDRIYHLKP 132

Query: 132 ITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLWW 191
           ITFSGPCNSPL+FKIEGTIKASPHISDYEKDRRHWIIFQN+S+F VEGKGIINGNGR WW
Sbjct: 133 ITFSGPCNSPLLFKIEGTIKASPHISDYEKDRRHWIIFQNISNFRVEGKGIINGNGRKWW 192

Query: 192 LNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYAP 251
            NSCKVNKTL       AVTFY+CTNLRVEG+RFRNAQ+MHLSFQKCNNVKALNLWIYAP
Sbjct: 193 SNSCKVNKTLPCKEAPTAVTFYECTNLRVEGIRFRNAQRMHLSFQKCNNVKALNLWIYAP 252

Query: 252 GNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGA 311
           GNSPNTDGIHVT TQ IVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGA
Sbjct: 253 GNSPNTDGIHVTGTQSIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGA 312

Query: 312 GKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYC 371
           GKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYC
Sbjct: 313 GKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYC 372

Query: 372 DQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQD 431
           DQKEPCTQQADAVQVSNV+YQNIRGTSASEVAVKFDCSKSFPCQG++LQDINLV +GK D
Sbjct: 373 DQKEPCTQQADAVQVSNVMYQNIRGTSASEVAVKFDCSKSFPCQGILLQDINLVRKGKND 432

Query: 432 DKSAQAEASCKNVKLKNRGRVSPQCAGR 452
           DKS QAEASCKNVKLKNRGRVSPQCAGR
Sbjct: 433 DKSTQAEASCKNVKLKNRGRVSPQCAGR 458

BLAST of Lag0002771 vs. NCBI nr
Match: XP_022991888.1 (polygalacturonase QRT2-like [Cucurbita maxima])

HSP 1 Score: 778.5 bits (2009), Expect = 3.2e-221
Identity = 388/455 (85.27%), Postives = 413/455 (90.77%), Query Frame = 0

Query: 7   KCSHNLLLLLCLLI-VVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYE-YSSEFSS 66
           +C   LLLLL  LI +VVF FFS C  SYAD+ D P+ I+ GDGKD D  Y  YSS+FSS
Sbjct: 9   RCRCRLLLLLPFLIAIVVFEFFSLCSSSYADV-DPPLLINRGDGKDRDQQYHGYSSKFSS 68

Query: 67  MLKTKLEKMAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDR 126
           ML T+LEKMAPSPV+S +EIFNVDDYGA+GDGEDDSEAFKEAWKEACSSTN IFLVP DR
Sbjct: 69  MLTTRLEKMAPSPVAS-SEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDR 128

Query: 127 TYHLKPITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIING 186
           TYHLKPITFSGPCNSP  FKIEGTIKASPHISDYEKDRRHWIIF+N+++FTVEGKGIING
Sbjct: 129 TYHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLTEFTVEGKGIING 188

Query: 187 NGRLWWLNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALN 246
           NGR WWLNSCKVNKTL       AVTFYQCTNLRVEGLRFRNAQKMHLSFQKC+NVKALN
Sbjct: 189 NGRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALN 248

Query: 247 LWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS 306
           LWIYAPGNSPNTDGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS
Sbjct: 249 LWIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS 308

Query: 307 IGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPII 366
           IGSLGAGKSEAEVS+VVVDTAKF+GTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPII
Sbjct: 309 IGSLGAGKSEAEVSDVVVDTAKFTGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPII 368

Query: 367 IDQNYCDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLV 426
           IDQNYCDQKEPCTQQA+AVQVSNV+YQNIRGTS SEVAVKFDCSKSFPC  ++LQDI+LV
Sbjct: 369 IDQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCLEILLQDIDLV 428

Query: 427 HRGKQDDKSAQAEASCKNVKLKNRGRVSPQCAGRR 453
           HR K DDKS QAEASCKN +LKNRGRVSPQC GRR
Sbjct: 429 HRDKTDDKSTQAEASCKNARLKNRGRVSPQCPGRR 461

BLAST of Lag0002771 vs. NCBI nr
Match: XP_022953263.1 (polygalacturonase QRT2-like [Cucurbita moschata])

HSP 1 Score: 775.0 bits (2000), Expect = 3.5e-220
Identity = 385/449 (85.75%), Postives = 408/449 (90.87%), Query Frame = 0

Query: 12  LLLLLCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHD-LHYEYSSEFSSMLKTKL 71
           LLLL  L+ +VVFGFFS C  SYAD+ D P  I  GDGKD D  HY YSS+FSSML T+L
Sbjct: 13  LLLLPFLVAIVVFGFFSVCSSSYADV-DPPPLIIRGDGKDRDHQHYGYSSKFSSMLTTRL 72

Query: 72  EKMAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDRTYHLKP 131
           EKMA SPV+S +EIFNVDDYGA+GDGEDDSEAFKEAWKEACSSTN IFLVP DRTYHLKP
Sbjct: 73  EKMASSPVAS-SEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPIDRTYHLKP 132

Query: 132 ITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLWW 191
           ITFSGPCNSP  FKIEGTIKASPHISDYEKDRRHWIIF+N+S+FTVEGKG INGNGR WW
Sbjct: 133 ITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEGKGTINGNGRKWW 192

Query: 192 LNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYAP 251
           LNSCKVNKTL       AVTFYQCTNLRVEGLRFRNAQKMHLSFQKC+NVKALNLWIYAP
Sbjct: 193 LNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALNLWIYAP 252

Query: 252 GNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGA 311
           GNSPNTDGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGA
Sbjct: 253 GNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGA 312

Query: 312 GKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYC 371
           GKSEAEVSNVVVDTAKF+GTSNGVRIKTWQGGKGYA+NIIFQNIVMDNVTNPIIIDQNYC
Sbjct: 313 GKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYC 372

Query: 372 DQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQD 431
           DQKEPCTQQA+AVQVSNV+YQNIRGTS SEVAVKFDCS+SFPCQ ++LQDI+LVHR K D
Sbjct: 373 DQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSESFPCQEILLQDIDLVHRDKAD 432

Query: 432 DKSAQAEASCKNVKLKNRGRVSPQCAGRR 453
           DK  QAEASCKN +LKNRGRVSPQC GRR
Sbjct: 433 DKPTQAEASCKNARLKNRGRVSPQCPGRR 459

BLAST of Lag0002771 vs. NCBI nr
Match: XP_023547692.1 (polygalacturonase QRT2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 772.7 bits (1994), Expect = 1.7e-219
Identity = 380/443 (85.78%), Postives = 405/443 (91.42%), Query Frame = 0

Query: 18  LLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHD-LHYEYSSEFSSMLKTKLEKMAPS 77
           L+ +VVF FFSAC  SYAD+ D P  I+ GDGKD D  HY YSS+FSSML T+LEKMA S
Sbjct: 15  LIAIVVFEFFSACSSSYADV-DPPPLINRGDGKDRDHQHYGYSSKFSSMLTTRLEKMASS 74

Query: 78  PVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDRTYHLKPITFSGP 137
           PV+S +EIFNVDDYGA+GDGEDDS+AFKEAWKEACSSTN IFLVP DRTYHLKPITFSGP
Sbjct: 75  PVAS-SEIFNVDDYGAMGDGEDDSQAFKEAWKEACSSTNAIFLVPNDRTYHLKPITFSGP 134

Query: 138 CNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLWWLNSCKV 197
           CNSP VFKIEGTIKASPHISDYEKDRRHWI+F+N+++FTVEGKG INGNGR WWLNSCKV
Sbjct: 135 CNSPFVFKIEGTIKASPHISDYEKDRRHWIVFENLAEFTVEGKGTINGNGRKWWLNSCKV 194

Query: 198 NKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYAPGNSPNT 257
           NKTL       AVTFYQCTNLRVEGLRFRNAQKMHLSFQKC+NVKALNLWIYAPGNSPNT
Sbjct: 195 NKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALNLWIYAPGNSPNT 254

Query: 258 DGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAE 317
           DGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAE
Sbjct: 255 DGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAE 314

Query: 318 VSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYCDQKEPC 377
           VSNVVVDTAKF GTSNGVRIKTWQGGKGYA+NIIFQNIVMDNVTNPIIIDQNYCDQKEPC
Sbjct: 315 VSNVVVDTAKFKGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPC 374

Query: 378 TQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQDDKSAQA 437
           TQQA+AVQVSNV+YQNIRGTS SEVAVKFDCSKSFPCQ ++LQDI+LVHR K DDK  QA
Sbjct: 375 TQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVHRDKADDKPTQA 434

Query: 438 EASCKNVKLKNRGRVSPQCAGRR 453
           EASCKN +LKNRGRVSPQC GRR
Sbjct: 435 EASCKNARLKNRGRVSPQCPGRR 455

BLAST of Lag0002771 vs. NCBI nr
Match: XP_004135935.1 (polygalacturonase QRT2 isoform X2 [Cucumis sativus] >KAE8646400.1 hypothetical protein Csa_016612 [Cucumis sativus])

HSP 1 Score: 768.5 bits (1983), Expect = 3.3e-218
Identity = 375/446 (84.08%), Postives = 404/446 (90.58%), Query Frame = 0

Query: 13  LLLLCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYEYSSEFSSMLKTKLEK 72
           LL    ++VV+F   S C GSY D+ +   SISSGD +    HY+YSSEFSSML+T+LEK
Sbjct: 14  LLTFLFVVVVIFDILSTCSGSYPDVDNPLPSISSGDKEVDGQHYKYSSEFSSMLRTRLEK 73

Query: 73  MAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDRTYHLKPIT 132
           MA SP++SP EIFNVDDYGA+GDGEDDSEAFKE WK+ACSSTN IFLVP DR YHLKPIT
Sbjct: 74  MASSPLASP-EIFNVDDYGAMGDGEDDSEAFKETWKDACSSTNAIFLVPCDRVYHLKPIT 133

Query: 133 FSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLWWLN 192
           F+GPCNSPL+FKIEGTIKASPHISDYEKDRRHWIIFQN+    VEGKGIINGNGR WWLN
Sbjct: 134 FTGPCNSPLLFKIEGTIKASPHISDYEKDRRHWIIFQNLLGLRVEGKGIINGNGRKWWLN 193

Query: 193 SCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYAPGN 252
           SCKVNKTL       AVTF+QCTNLRVEGLRFRNAQ+MHLSFQKCNNVKALNLWI++PGN
Sbjct: 194 SCKVNKTLPCKEAPTAVTFFQCTNLRVEGLRFRNAQQMHLSFQKCNNVKALNLWIHSPGN 253

Query: 253 SPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGK 312
           SPNTDGIHVT TQFIVIKNCLIMTGDDC+SIVSGSKNVRAKGITCGPGHGISIGSLGAGK
Sbjct: 254 SPNTDGIHVTGTQFIVIKNCLIMTGDDCLSIVSGSKNVRAKGITCGPGHGISIGSLGAGK 313

Query: 313 SEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYCDQ 372
           +EAEVSNVVVDTAKFSGT+NGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYCDQ
Sbjct: 314 TEAEVSNVVVDTAKFSGTTNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYCDQ 373

Query: 373 KEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQDDK 432
           KEPCTQQADAV+VSNV+YQNIRGTSASEVAVKF+CSKS PCQG++LQDINLVH+GK DDK
Sbjct: 374 KEPCTQQADAVEVSNVVYQNIRGTSASEVAVKFNCSKSIPCQGILLQDINLVHKGKNDDK 433

Query: 433 SAQAEASCKNVKLKNRGRVSPQCAGR 452
           SAQAEASCKNVKLKNRGRVSP C GR
Sbjct: 434 SAQAEASCKNVKLKNRGRVSPLCGGR 458

BLAST of Lag0002771 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.1e-127
Identity = 222/378 (58.73%), Postives = 276/378 (73.02%), Query Frame = 0

Query: 78  VSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDRTYHLKPITFSGPC 137
           V   ++  NVDD+GA GDG DD++AF++AWK ACSST+   L+   + Y ++PI+FSGPC
Sbjct: 83  VLDASKTVNVDDFGAKGDGRDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPC 142

Query: 138 NSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLWWLNSCKVN 197
            S L  +I GTI+AS   SDY KD RHW++F ++ +  VEG G INGNG++WW NSCK N
Sbjct: 143 KSGLTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTN 202

Query: 198 KTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYAPGNSPNTD 257
           K L       A+TFY+  ++ V+ L+  NAQ++H+SF  C NV+A NL + AP NSPNTD
Sbjct: 203 KALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTD 262

Query: 258 GIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEV 317
           GIHVT TQ I I +C+I TGDDCISIV+GS+ VR   ITCGPGHGISIGSLG G SEA V
Sbjct: 263 GIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHV 322

Query: 318 SNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYCDQKEPCT 377
           S+VVV+ AK  GT+NGVRIKTWQGG G A NI FQN+ M NV NPIIIDQNYCDQ +PC 
Sbjct: 323 SDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQ 382

Query: 378 QQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQDDKSAQAE 437
           +Q+ AVQV NV+YQNI+GT AS VA+ FDCSK FPCQG++L+D++L   G      A A+
Sbjct: 383 EQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEG-----GAAAK 442

Query: 438 ASCKNVKLKNRGRVSPQC 449
           A C NV+L   G VSP C
Sbjct: 443 ALCNNVELSETGVVSPHC 455

BLAST of Lag0002771 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 451.8 bits (1161), Expect = 9.0e-126
Identity = 238/446 (53.36%), Postives = 312/446 (69.96%), Query Frame = 0

Query: 16  LCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYEYSSEFSSMLKTK----LE 75
           + +L V +  F  +C  SY      P +         +++YE + +      T+      
Sbjct: 5   IIILSVFLLTFLPSCFSSY------PFNHRDDLFMSSNVYYETNRQHQHGHNTRNSHLKN 64

Query: 76  KMAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLV-PWDRTYHLKP 135
           +   +P SSP   FNV+ +GA  +G DDS+AF +AW+ ACSST  +++V P +R Y LK 
Sbjct: 65  RHGYAPRSSPRS-FNVNTFGAKANGNDDSKAFMKAWEAACSSTGIVYIVAPKNRDYMLKA 124

Query: 136 ITFSGPCNSPL-VFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLW 195
           +TFSGPC S L +FKI G I+A  + SDY K+RRHWI+F+N+++  VEG G I+GNG +W
Sbjct: 125 VTFSGPCKSSLIIFKIYGRIEAWENPSDY-KERRHWIVFENVNNLRVEGGGRIDGNGHIW 184

Query: 196 WLNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYA 255
           W  SCK+N  L       AVTF +C NLRV  +R  NAQ+MHL+FQ C NVKALNL + +
Sbjct: 185 WPKSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTS 244

Query: 256 PGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLG 315
           P +SPNTDGIHV+ TQ I+I++ ++ TGDDCISIVSGS+NVRA GITCGPGHGISIGSLG
Sbjct: 245 PADSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLG 304

Query: 316 AGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNY 375
              SEA VSNVVV+ A   GT+NGVRIKTWQGG G A+NIIFQ+I+M NVTNPIII+Q+Y
Sbjct: 305 EDNSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDY 364

Query: 376 CDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQ 435
           CD+ E C +Q  AVQVSNV+Y+NI+GTS+  +AVKF CSK+ PC+G+ +Q++ LV + +Q
Sbjct: 365 CDRVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQ 424

Query: 436 DDKSAQAEASCKNVKLKNRGRVSPQC 449
           D     ++ASC NVKL  RG VSP C
Sbjct: 425 D----VSKASCSNVKLDTRGNVSPLC 438

BLAST of Lag0002771 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 3.1e-118
Identity = 224/449 (49.89%), Postives = 301/449 (67.04%), Query Frame = 0

Query: 18  LLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYEYSSEFSSML---------KT 77
           LL++++F    + C S     +L   +      D+ L  E++ +F + L           
Sbjct: 9   LLLIIIFASSISTCRSNVIDDNLFKQV-----YDNILEQEFAHDFQAYLSYLSKNIESNN 68

Query: 78  KLEKMAPSPVSSPAEIFNVDDYGALGDGED-DSEAFKEAWKEACSSTNGI-FLVPWDRTY 137
            ++K+  + +    ++ NV  +GA GDG+  D+ AF++AW EACSS   + F+VP ++ Y
Sbjct: 69  NIDKVDKNGI----KVINVLSFGAKGDGKTYDNIAFEQAWNEACSSRTPVQFVVPKNKNY 128

Query: 138 HLKPITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNG 197
            LK ITFSGPC S +  KI G+++AS  ISDY KDRR WI F ++ +  V G G INGNG
Sbjct: 129 LLKQITFSGPCRSSISVKIFGSLEASSKISDY-KDRRLWIAFDSVQNLVVGGGGTINGNG 188

Query: 198 RLWWLNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLW 257
           ++WW +SCK+NK+L       A+TF+ C NL+V  L+ +NAQ++H+ F+ C NV A NL 
Sbjct: 189 QVWWPSSCKINKSLPCRDAPTALTFWNCKNLKVNNLKSKNAQQIHIKFESCTNVVASNLM 248

Query: 258 IYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIG 317
           I A   SPNTDG+HV+ TQ+I I + +I TGDDCISIVSGS+NV+A  ITCGPGHGISIG
Sbjct: 249 INASAKSPNTDGVHVSNTQYIQISDTIIGTGDDCISIVSGSQNVQATNITCGPGHGISIG 308

Query: 318 SLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIID 377
           SLG+G SEA VSNV V+ AK  G  NGVRIKTWQGG G A NI F N+ M +V  PIIID
Sbjct: 309 SLGSGNSEAYVSNVTVNEAKIIGAENGVRIKTWQGGSGQASNIKFLNVEMQDVKYPIIID 368

Query: 378 QNYCDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHR 437
           QNYCD+ EPC QQ  AVQV NV+Y+NI+GTSA++VA+KFDCS +FPC+G+I+++INLV  
Sbjct: 369 QNYCDRVEPCIQQFSAVQVKNVVYENIKGTSATKVAIKFDCSTNFPCEGIIMENINLV-- 428

Query: 438 GKQDDKSAQAEASCKNVKLKNRGRVSPQC 449
               +    +EA+CKNV   N   V+P C
Sbjct: 429 ---GESGKPSEATCKNVHFNNAEHVTPHC 442

BLAST of Lag0002771 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.2e-114
Identity = 234/444 (52.70%), Postives = 290/444 (65.32%), Query Frame = 0

Query: 12  LLLLLCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYEY----SSEFSSMLK 71
           L  LL  + ++ F F+S+      D   L + ++   G D   +  Y      EFSS++ 
Sbjct: 3   LTRLLLPISILWFCFYSSHTILQKD--PLLICVNGDPGFDQRAYPTYFGPILDEFSSIMG 62

Query: 72  -----TKLEKMAP--SPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLV 131
                  LE+  P   P +SP    +VDD+GA GDG DD++AF++AWK+ACSS   + +V
Sbjct: 63  FEPSILSLERFNPVGGPETSPDTDISVDDFGARGDGTDDTKAFEKAWKDACSS-GSVLIV 122

Query: 132 PWDRTYHLKPITFSGPCNSPLVFKIEGTIKASPHISDY-EKDRRHWIIFQNMSDFTVEGK 191
           P ++ Y LK ITFSGPC S L  KI GTI+AS   SD+   +R+ WI F+++S+ T+EG 
Sbjct: 123 PENKNYLLKQITFSGPCKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGG 182

Query: 192 GIINGNGRLWWLNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNN 251
           G  NGNG  WW +SCK  K+L       A+TF  C NL V  L  +++QKMHLSF KC +
Sbjct: 183 GTSNGNGETWWDSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQD 242

Query: 252 VKALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGP 311
           V A NL + AP +SPNTDGIH+T TQ I + N +I TGDDCISI SGSK V A  ITCGP
Sbjct: 243 VIASNLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGP 302

Query: 312 GHGISIGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNV 371
           GHGISIGSLG   SEA VS V+VD      T+NG+RIKTWQGG G A+NI FQNIVM NV
Sbjct: 303 GHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNV 362

Query: 372 TNPIIIDQNYCDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQ 431
           TNPIIIDQ YCD K+PC +Q  AV+VSNV Y NIRGTSASEVAVKFDCSKS PCQG I+ 
Sbjct: 363 TNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVG 422

Query: 432 DINLVHRGKQDDKSAQAEASCKNV 437
           +INLV  G       +   SC N+
Sbjct: 423 NINLVGNG-----GKETTMSCSNI 438

BLAST of Lag0002771 vs. ExPASy Swiss-Prot
Match: P48978 (Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 9.6e-112
Identity = 209/379 (55.15%), Postives = 261/379 (68.87%), Query Frame = 0

Query: 79  SSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDRTYHLKPITFSGPCN 138
           S+PA+  +VDD+GA G+G DD++AF +AWK ACSS+  + LV   + Y ++PI FSGPC 
Sbjct: 93  SAPAKTISVDDFGAKGNGADDTQAFVKAWKAACSSSGAMVLVVPQKNYLVRPIEFSGPCK 152

Query: 139 SPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLWWLNSCKVN- 198
           S L  +I GTI+AS   S Y KD  HW+IF N+ +  V G G INGNG +WW NSCK+  
Sbjct: 153 SQLTLQIYGTIEASEDRSIY-KDIDHWLIFDNVQNLLVVGPGTINGNGNIWWKNSCKIKP 212

Query: 199 -------KTLAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYAPGNSPNTD 258
                     AVTF +C NL V+ L  ++AQ++H+ FQ C NV+A  L + AP +SPNTD
Sbjct: 213 QPPCGTYAPTAVTFNRCNNLVVKNLNIQDAQQIHVIFQNCINVQASCLTVTAPEDSPNTD 272

Query: 259 GIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEV 318
           GIHVT TQ I I + +I TGDDCISIVSGS+ V+A  ITCGPGHGISIGSLG   SE  V
Sbjct: 273 GIHVTNTQNITISSSVIGTGDDCISIVSGSQRVQATDITCGPGHGISIGSLGEDGSEDHV 332

Query: 319 SNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYCDQK-EPC 378
           S V V+ AK SGTSNG+RIKTW+GG G A NI+FQN+ M++VTNPIIIDQNYCD K + C
Sbjct: 333 SGVFVNGAKLSGTSNGLRIKTWKGGSGSATNIVFQNVQMNDVTNPIIIDQNYCDHKTKDC 392

Query: 379 TQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQDDKSAQA 438
            QQ  AVQV NV+YQNIRGTSAS  A+  +CS+S PCQG++LQ + L             
Sbjct: 393 KQQKSAVQVKNVLYQNIRGTSASGDAITLNCSQSVPCQGIVLQSVQL----------QNG 452

Query: 439 EASCKNVKLKNRGRVSPQC 449
            A C NV+   +G VSP+C
Sbjct: 453 RAECNNVQPAYKGVVSPRC 460

BLAST of Lag0002771 vs. ExPASy TrEMBL
Match: A0A6J1JXJ9 (polygalacturonase QRT2-like OS=Cucurbita maxima OX=3661 GN=LOC111488393 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.5e-221
Identity = 388/455 (85.27%), Postives = 413/455 (90.77%), Query Frame = 0

Query: 7   KCSHNLLLLLCLLI-VVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYE-YSSEFSS 66
           +C   LLLLL  LI +VVF FFS C  SYAD+ D P+ I+ GDGKD D  Y  YSS+FSS
Sbjct: 9   RCRCRLLLLLPFLIAIVVFEFFSLCSSSYADV-DPPLLINRGDGKDRDQQYHGYSSKFSS 68

Query: 67  MLKTKLEKMAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDR 126
           ML T+LEKMAPSPV+S +EIFNVDDYGA+GDGEDDSEAFKEAWKEACSSTN IFLVP DR
Sbjct: 69  MLTTRLEKMAPSPVAS-SEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDR 128

Query: 127 TYHLKPITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIING 186
           TYHLKPITFSGPCNSP  FKIEGTIKASPHISDYEKDRRHWIIF+N+++FTVEGKGIING
Sbjct: 129 TYHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLTEFTVEGKGIING 188

Query: 187 NGRLWWLNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALN 246
           NGR WWLNSCKVNKTL       AVTFYQCTNLRVEGLRFRNAQKMHLSFQKC+NVKALN
Sbjct: 189 NGRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALN 248

Query: 247 LWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS 306
           LWIYAPGNSPNTDGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS
Sbjct: 249 LWIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS 308

Query: 307 IGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPII 366
           IGSLGAGKSEAEVS+VVVDTAKF+GTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPII
Sbjct: 309 IGSLGAGKSEAEVSDVVVDTAKFTGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPII 368

Query: 367 IDQNYCDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLV 426
           IDQNYCDQKEPCTQQA+AVQVSNV+YQNIRGTS SEVAVKFDCSKSFPC  ++LQDI+LV
Sbjct: 369 IDQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCLEILLQDIDLV 428

Query: 427 HRGKQDDKSAQAEASCKNVKLKNRGRVSPQCAGRR 453
           HR K DDKS QAEASCKN +LKNRGRVSPQC GRR
Sbjct: 429 HRDKTDDKSTQAEASCKNARLKNRGRVSPQCPGRR 461

BLAST of Lag0002771 vs. ExPASy TrEMBL
Match: A0A6J1GMX8 (polygalacturonase QRT2-like OS=Cucurbita moschata OX=3662 GN=LOC111455865 PE=3 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 1.7e-220
Identity = 385/449 (85.75%), Postives = 408/449 (90.87%), Query Frame = 0

Query: 12  LLLLLCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHD-LHYEYSSEFSSMLKTKL 71
           LLLL  L+ +VVFGFFS C  SYAD+ D P  I  GDGKD D  HY YSS+FSSML T+L
Sbjct: 13  LLLLPFLVAIVVFGFFSVCSSSYADV-DPPPLIIRGDGKDRDHQHYGYSSKFSSMLTTRL 72

Query: 72  EKMAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDRTYHLKP 131
           EKMA SPV+S +EIFNVDDYGA+GDGEDDSEAFKEAWKEACSSTN IFLVP DRTYHLKP
Sbjct: 73  EKMASSPVAS-SEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPIDRTYHLKP 132

Query: 132 ITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLWW 191
           ITFSGPCNSP  FKIEGTIKASPHISDYEKDRRHWIIF+N+S+FTVEGKG INGNGR WW
Sbjct: 133 ITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEGKGTINGNGRKWW 192

Query: 192 LNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYAP 251
           LNSCKVNKTL       AVTFYQCTNLRVEGLRFRNAQKMHLSFQKC+NVKALNLWIYAP
Sbjct: 193 LNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALNLWIYAP 252

Query: 252 GNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGA 311
           GNSPNTDGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGA
Sbjct: 253 GNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGA 312

Query: 312 GKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYC 371
           GKSEAEVSNVVVDTAKF+GTSNGVRIKTWQGGKGYA+NIIFQNIVMDNVTNPIIIDQNYC
Sbjct: 313 GKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYC 372

Query: 372 DQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQD 431
           DQKEPCTQQA+AVQVSNV+YQNIRGTS SEVAVKFDCS+SFPCQ ++LQDI+LVHR K D
Sbjct: 373 DQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSESFPCQEILLQDIDLVHRDKAD 432

Query: 432 DKSAQAEASCKNVKLKNRGRVSPQCAGRR 453
           DK  QAEASCKN +LKNRGRVSPQC GRR
Sbjct: 433 DKPTQAEASCKNARLKNRGRVSPQCPGRR 459

BLAST of Lag0002771 vs. ExPASy TrEMBL
Match: O81246 (Polygalacturonase OS=Cucumis melo OX=3656 GN=MPG3 PE=2 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.6e-216
Identity = 379/456 (83.11%), Postives = 404/456 (88.60%), Query Frame = 0

Query: 4   GFEKCSHNLLLLLCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHY--EYSSE 63
           G   C  +LL  L  ++VV F  FS C GSY D+ +   SISSGD +D+  HY    SSE
Sbjct: 7   GRRPCCLSLLTFL-FVVVVNFDTFSTCFGSYPDVDNPLPSISSGDKEDYGQHYYEHSSSE 66

Query: 64  FSSMLKTKLEKMAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVP 123
           FSSML+T+LEKM  SP++SP EIFNVDDYGA+GDGEDD+EAFKE WK+ACSSTN IFLVP
Sbjct: 67  FSSMLRTRLEKMVSSPLASP-EIFNVDDYGAMGDGEDDTEAFKETWKDACSSTNAIFLVP 126

Query: 124 WDRTYHLKPITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGI 183
            DR YHLKPITFSGPCNSPL+F+IEGT+KASPHISDYEKDRRHWIIFQN+    VEGKGI
Sbjct: 127 CDRVYHLKPITFSGPCNSPLLFQIEGTVKASPHISDYEKDRRHWIIFQNLLGLRVEGKGI 186

Query: 184 INGNGRLWWLNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVK 243
           INGNGR WWLNSCKVNKTL       AVTFYQCTNLRVEGLRFRNAQ+M LSFQ+CNNVK
Sbjct: 187 INGNGRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQQMLLSFQRCNNVK 246

Query: 244 ALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGH 303
           ALNLWIYAPGNSPNTDGIHVT TQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGH
Sbjct: 247 ALNLWIYAPGNSPNTDGIHVTGTQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGH 306

Query: 304 GISIGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTN 363
           GISIGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTN
Sbjct: 307 GISIGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTN 366

Query: 364 PIIIDQNYCDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDI 423
           PIII+QNYCDQKEPCTQQADAV VSNV+YQNIRGTSASEVAVKFDCSKS PCQG++LQDI
Sbjct: 367 PIIINQNYCDQKEPCTQQADAVAVSNVMYQNIRGTSASEVAVKFDCSKSVPCQGILLQDI 426

Query: 424 NLVHRGKQDDKSAQAEASCKNVKLKNRGRVSPQCAG 451
           NLVH+GK DDKSAQAEASCKNVK KNRGRVSPQC G
Sbjct: 427 NLVHKGKNDDKSAQAEASCKNVKWKNRGRVSPQCGG 460

BLAST of Lag0002771 vs. ExPASy TrEMBL
Match: A0A6J1CWK1 (polygalacturonase QRT2 OS=Momordica charantia OX=3673 GN=LOC111015052 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.4e-216
Identity = 381/451 (84.48%), Postives = 407/451 (90.24%), Query Frame = 0

Query: 12  LLLLLCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYEYSSEF--SSMLKTK 71
           LLLL  L+ V+V   +S    S+A + D P S SSGDG DHD HYEYSSE   S +L TK
Sbjct: 10  LLLLPLLVTVLVSDLYST---SHAAV-DPPPSFSSGDGVDHDRHYEYSSELLDSFVLTTK 69

Query: 72  LEKMAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDRTYHLK 131
           LE+ APSPV+SP EIFNVDD+GA+GDGEDDSEAFKEAWKEACSSTNGI LVP++RTYHLK
Sbjct: 70  LERGAPSPVASP-EIFNVDDFGAMGDGEDDSEAFKEAWKEACSSTNGILLVPYERTYHLK 129

Query: 132 PITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLW 191
           PITFSGPCNSPLVFKI+GTIKASPH+SDYEKDRRHWI+FQNM DFTV+G G INGNGR W
Sbjct: 130 PITFSGPCNSPLVFKIDGTIKASPHLSDYEKDRRHWIVFQNMEDFTVKGDGTINGNGRKW 189

Query: 192 WLNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYA 251
           WLNSCKVNKTL       AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYA
Sbjct: 190 WLNSCKVNKTLPCKDAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYA 249

Query: 252 PGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLG 311
           PGNSPNTDGIHVT TQFIVI+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLG
Sbjct: 250 PGNSPNTDGIHVTGTQFIVIENCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLG 309

Query: 312 AGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNY 371
           AGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVM+NV+NPIIIDQNY
Sbjct: 310 AGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMNNVSNPIIIDQNY 369

Query: 372 CDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQ 431
           CD+KE C QQADAVQVSNV Y+NIRGTSASEVAVKFDCSKS PCQG++LQD+NLVHRGK 
Sbjct: 370 CDRKERCKQQADAVQVSNVKYRNIRGTSASEVAVKFDCSKSLPCQGILLQDVNLVHRGKG 429

Query: 432 DDKSAQAEASCKNVKLKNRGRVSPQCAGRRK 454
           D KSA+AEASCKNVKLKNRG VSPQCAGRR+
Sbjct: 430 DHKSAEAEASCKNVKLKNRGSVSPQCAGRRR 455

BLAST of Lag0002771 vs. ExPASy TrEMBL
Match: A0A0A0K6J9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G427620 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.2e-178
Identity = 310/372 (83.33%), Postives = 334/372 (89.78%), Query Frame = 0

Query: 13  LLLLCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYEYSSEFSSMLKTKLEK 72
           LL    ++VV+F   S C GSY D+ +   SISSGD +    HY+YSSEFSSML+T+LEK
Sbjct: 14  LLTFLFVVVVIFDILSTCSGSYPDVDNPLPSISSGDKEVDGQHYKYSSEFSSMLRTRLEK 73

Query: 73  MAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLVPWDRTYHLKPIT 132
           MA SP++SP EIFNVDDYGA+GDGEDDSEAFKE WK+ACSSTN IFLVP DR YHLKPIT
Sbjct: 74  MASSPLASP-EIFNVDDYGAMGDGEDDSEAFKETWKDACSSTNAIFLVPCDRVYHLKPIT 133

Query: 133 FSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLWWLN 192
           F+GPCNSPL+FKIEGTIKASPHISDYEKDRRHWIIFQN+    VEGKGIINGNGR WWLN
Sbjct: 134 FTGPCNSPLLFKIEGTIKASPHISDYEKDRRHWIIFQNLLGLRVEGKGIINGNGRKWWLN 193

Query: 193 SCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYAPGN 252
           SCKVNKTL       AVTF+QCTNLRVEGLRFRNAQ+MHLSFQKCNNVKALNLWI++PGN
Sbjct: 194 SCKVNKTLPCKEAPTAVTFFQCTNLRVEGLRFRNAQQMHLSFQKCNNVKALNLWIHSPGN 253

Query: 253 SPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGK 312
           SPNTDGIHVT TQFIVIKNCLIMTGDDC+SIVSGSKNVRAKGITCGPGHGISIGSLGAGK
Sbjct: 254 SPNTDGIHVTGTQFIVIKNCLIMTGDDCLSIVSGSKNVRAKGITCGPGHGISIGSLGAGK 313

Query: 313 SEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYCDQ 372
           +EAEVSNVVVDTAKFSGT+NGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYCDQ
Sbjct: 314 TEAEVSNVVVDTAKFSGTTNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNYCDQ 373

Query: 373 KEPCTQQADAVQ 378
           KEPCTQQADAV+
Sbjct: 374 KEPCTQQADAVE 384

BLAST of Lag0002771 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 451.8 bits (1161), Expect = 6.4e-127
Identity = 238/446 (53.36%), Postives = 312/446 (69.96%), Query Frame = 0

Query: 16  LCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYEYSSEFSSMLKTK----LE 75
           + +L V +  F  +C  SY      P +         +++YE + +      T+      
Sbjct: 5   IIILSVFLLTFLPSCFSSY------PFNHRDDLFMSSNVYYETNRQHQHGHNTRNSHLKN 64

Query: 76  KMAPSPVSSPAEIFNVDDYGALGDGEDDSEAFKEAWKEACSSTNGIFLV-PWDRTYHLKP 135
           +   +P SSP   FNV+ +GA  +G DDS+AF +AW+ ACSST  +++V P +R Y LK 
Sbjct: 65  RHGYAPRSSPRS-FNVNTFGAKANGNDDSKAFMKAWEAACSSTGIVYIVAPKNRDYMLKA 124

Query: 136 ITFSGPCNSPL-VFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEGKGIINGNGRLW 195
           +TFSGPC S L +FKI G I+A  + SDY K+RRHWI+F+N+++  VEG G I+GNG +W
Sbjct: 125 VTFSGPCKSSLIIFKIYGRIEAWENPSDY-KERRHWIVFENVNNLRVEGGGRIDGNGHIW 184

Query: 196 WLNSCKVNKTL-------AVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWIYA 255
           W  SCK+N  L       AVTF +C NLRV  +R  NAQ+MHL+FQ C NVKALNL + +
Sbjct: 185 WPKSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTS 244

Query: 256 PGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLG 315
           P +SPNTDGIHV+ TQ I+I++ ++ TGDDCISIVSGS+NVRA GITCGPGHGISIGSLG
Sbjct: 245 PADSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLG 304

Query: 316 AGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQNY 375
              SEA VSNVVV+ A   GT+NGVRIKTWQGG G A+NIIFQ+I+M NVTNPIII+Q+Y
Sbjct: 305 EDNSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDY 364

Query: 376 CDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRGKQ 435
           CD+ E C +Q  AVQVSNV+Y+NI+GTS+  +AVKF CSK+ PC+G+ +Q++ LV + +Q
Sbjct: 365 CDRVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQ 424

Query: 436 DDKSAQAEASCKNVKLKNRGRVSPQC 449
           D     ++ASC NVKL  RG VSP C
Sbjct: 425 D----VSKASCSNVKLDTRGNVSPLC 438

BLAST of Lag0002771 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 395.6 bits (1015), Expect = 5.4e-110
Identity = 217/459 (47.28%), Postives = 302/459 (65.80%), Query Frame = 0

Query: 7   KCSHNLLLLLCLLIVVVFGFFSACCGSYADIMDLPVSISSGDGKDHDLHYEYSSEFSSML 66
           +C  +L + LC+L+++      + C + +  +D        DG  H+   + S E  S+L
Sbjct: 3   RCCRHLAVFLCVLLML------SLCKALSSNVD--------DGYGHE---DGSFESDSLL 62

Query: 67  KTK----LEKMAPSPVSSPAEIFNVDDYGALGDGE-DDSEAFKEAWKEACSSTNGI--FL 126
           K      L  ++    +  A   +V ++GA GDG+ DD++AFK+AWK+AC STNG+  FL
Sbjct: 63  KLNNDDVLSLISSDETTLEASTVSVSNFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFL 122

Query: 127 VPWDRTYHLKPITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEG- 186
           VP  +TY LK   F GPC S   F+I GT+ AS   SDY KD+ HW+I +++++ +++G 
Sbjct: 123 VPKGKTYLLKSTRFRGPCKSLRNFQILGTLSASTKRSDY-KDKNHWLILEDVNNLSIDGG 182

Query: 187 -KGIINGNGRLWWLNSCKVNKT-------LAVTFYQCTNLRVEGLRFRNAQKMHLSFQKC 246
             GIINGNG+ WW NSCK++K+        A+T Y   NL V+ LR +NAQ++ +S +KC
Sbjct: 183 STGIINGNGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKC 242

Query: 247 NNVKALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITC 306
           N V+  N+ I APG+SPNTDGIH+T TQ I + N  I TGDDCISI  G++N++   +TC
Sbjct: 243 NKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTC 302

Query: 307 GPGHGISIGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMD 366
           GPGHGISIGSLG   S+A VS + VD AKFS + NGVRIKT+QGG G A+NI FQNI M+
Sbjct: 303 GPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRME 362

Query: 367 NVTNPIIIDQNYCDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVI 426
           NV NPIIIDQ+YCD K+ C  Q  AVQV NV+Y+NI GTSA++VA+  +CS+ +PCQG++
Sbjct: 363 NVKNPIIIDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIV 422

Query: 427 LQDINLVHRGKQDDKSAQAEASCKNVKLKNRGRVSPQCA 450
           L+++          K     ASCKN  +KN+G VSP+C+
Sbjct: 423 LENV----------KIKGGTASCKNANVKNQGTVSPKCS 431

BLAST of Lag0002771 vs. TAIR 10
Match: AT2G41850.1 (polygalacturonase abscission zone A. thaliana )

HSP 1 Score: 385.6 bits (989), Expect = 5.6e-107
Identity = 203/392 (51.79%), Postives = 264/392 (67.35%), Query Frame = 0

Query: 68  TKLEKMAPSPVSSPAEIFNVDDYGALGDGE-DDSEAFKEAWKEACSSTNGI-FLVPWDRT 127
           T L     + + +P  + +V D+GA GDG+ DD++AF  AWK+ACSS   +  LVP   T
Sbjct: 52  TGLRSFVRASLRTPTTV-SVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNT 111

Query: 128 YHLKPITFSGPCNSPLVFKIEGTIKASPHISDYEKDRRHWIIFQNMSDFTVEG--KGIIN 187
           Y LK I  +GPCNS L  +I GT+ AS   SDY KD   WI+F  +++ +V+G   G+++
Sbjct: 112 YLLKSIQLTGPCNSILTVQIFGTLSASQKRSDY-KDISKWIMFDGVNNLSVDGGDTGVVD 171

Query: 188 GNGRLWWLNSCKVNKT-------LAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKAL 247
           GNG  WW NSCK NK         A+TFY   +L V+ L+ RNAQ++ +S +KC+NV+  
Sbjct: 172 GNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVS 231

Query: 248 NLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGI 307
           N+ + AP +SPNTDGIH+T TQ I +   +I TGDDCISI SGS+NV+   ITCGPGHGI
Sbjct: 232 NVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGI 291

Query: 308 SIGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPI 367
           SIGSLG   S+A VS V VD AK SGT NGVRIKT+QGG G A NIIFQNI MDNV NPI
Sbjct: 292 SIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPI 351

Query: 368 IIDQNYCDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINL 427
           IIDQ+YCD K  CT +  AVQV NV+Y++I GTSASE A+ F+CSK++PCQG++L  +N+
Sbjct: 352 IIDQDYCD-KSKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNI 411

Query: 428 VHRGKQDDKSAQAEASCKNVKLKNRGRVSPQC 449
                        +A+C N  + ++G V PQC
Sbjct: 412 ----------KGGKATCTNANVVDKGAVLPQC 430

BLAST of Lag0002771 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 3.0e-92
Identity = 178/398 (44.72%), Postives = 244/398 (61.31%), Query Frame = 0

Query: 59  SSEFSSMLKTKLEKMAPSPVSSPAEIFNVDDYGALGDG-EDDSEAFKEAWKEACSS-TNG 118
           ++ F S+L+    + + S       + +V ++GA G+G  DD++AF +AWK ACSS    
Sbjct: 28  ANSFESLLQLP-RRQSRSTRPRSERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKT 87

Query: 119 IFLVPWDRTYHLKPITFSGPCNSPLVFKIEGTIKASPHISDYE-KDRRHWIIFQNMSDFT 178
             LVP + T  L+PI  SGPC + L  +I GTI A      +E  +RR W+ F  +S  T
Sbjct: 88  RILVPENYTCLLRPIDLSGPCKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLT 147

Query: 179 VEGKGIINGNGRLWWLNSCKVNKT-------LAVTFYQCTNLRVEGLRFRNAQKMHLSFQ 238
           VEG G +NG G+ WW  SCK N +        A+TF++C N+RVE L   ++Q+MH++  
Sbjct: 148 VEGGGTVNGMGQEWWERSCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALT 207

Query: 239 KCNNVKALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGI 298
            C  V    L + AP  SPNTDGIH++ ++ IVI N  + TGDDCISIV  S  +    I
Sbjct: 208 SCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNI 267

Query: 299 TCGPGHGISIGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIV 358
            CGPGHGISIGSLG  KS  EV ++ VDTA  S T+NGVRIKTWQGG G    IIF+NI 
Sbjct: 268 ICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIK 327

Query: 359 MDNVTNPIIIDQNYCDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQG 418
           M+NV+NPIIIDQ YCD ++PC  Q  A+ + N+ + ++RGTSAS+ A+K  CS S PC+ 
Sbjct: 328 MNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRN 387

Query: 419 VILQDINLVHRGKQDDKSAQAEASCKNVKLKNRGRVSP 447
           ++LQDI+L    +  +     E+ C      + G+V P
Sbjct: 388 ILLQDIDL----EPSNGDGFTESFCWEAYGSSSGQVYP 420

BLAST of Lag0002771 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 335.1 bits (858), Expect = 8.7e-92
Identity = 179/446 (40.13%), Postives = 258/446 (57.85%), Query Frame = 0

Query: 12  LLLLLCLLIVVVFGFFSACCGSYADIMDL--PVSISSGDGKDHDLHYEYSSEFSSMLKTK 71
           ++ L  LL+V  +G          DI+     + +   D +D    +++SS F+S    K
Sbjct: 6   IISLFALLMVASYGATGKMLFKELDILGKLENLDVPEDDIEDDVTFFDFSS-FTSQYSGK 65

Query: 72  LEKMAPSPVSSPAEIFNVDDYGALGDG-EDDSEAFKEAWKEACSSTNGIFLVPWDRTYHL 131
                         + NVD + A GDG  DD++AF  AW  ACS+ N + LVP  R+Y +
Sbjct: 66  -------------NLVNVDSFNASGDGVSDDTQAFIRAWTMACSAPNSVLLVPQGRSYLV 125

Query: 132 KPITFSGPCNSPLVFKIEGTIKASPHISDYE-KDRRHWIIFQNMSDFTVEGKGIINGNGR 191
               F GPC   L+ +I+GTI A    S ++ K  R+W+ F  +     +G G+I+G+G 
Sbjct: 126 NATKFDGPCQEKLIIQIDGTIIAPDEPSQWDPKFPRNWLQFSKLQGVVFQGNGVIDGSGT 185

Query: 192 LWWLNSCKVNKT-------LAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNLWI 251
            WW  SCK NK+        A+T Y  +N+ V GL  RN+Q+MHL  Q+   V+   + +
Sbjct: 186 KWWAASCKKNKSNPCVGAPTALTIYSSSNVYVRGLTIRNSQQMHLIIQRSTTVRISRVMV 245

Query: 252 YAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGS 311
            +PG+SPNTDGIH+TA+  +V+++  I TGDDC+SIV+GS  ++ K I CGPGHGISIGS
Sbjct: 246 TSPGDSPNTDGIHITASTDVVVQDSKISTGDDCVSIVNGSAKIKMKRIYCGPGHGISIGS 305

Query: 312 LGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIIIDQ 371
           LG G S+  V+ VV++TA    T+NG+RIKTWQGG GY + + F+N+VM +V NPIIIDQ
Sbjct: 306 LGQGHSKGTVTAVVLETAFLKNTTNGLRIKTWQGGNGYVKGVRFENVVMQDVANPIIIDQ 365

Query: 372 NYCDQKEPCTQQADAVQVSNVIYQNIRGTSASEVAVKFDCSKSFPCQGVILQDINLVHRG 431
            YCD    C  Q  AV +S ++Y+NI GT+ S  A+ F CS + PC  ++L +INL    
Sbjct: 366 FYCDSPSTCQNQTSAVHISEIMYRNITGTTKSSKAINFKCSDAVPCSHIVLNNINL---- 425

Query: 432 KQDDKSAQAEASCKNVKLKNRGRVSP 447
             +    + EA C + +    G V P
Sbjct: 426 --EGNDGKVEAYCNSAEGFGYGVVHP 431