Lag0002765 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002765
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDNA-directed RNA polymerases II, IV and V subunit 11-like
Locationchr4: 45430298 .. 45432222 (-)
RNA-Seq ExpressionLag0002765
SyntenyLag0002765
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGTCCCCGATCGCTACGAGCGCTTCGTCGTCCCCGAGGGCGCCAAGAAGTAAATTCTCGAAACCCTAGCCTTTTCATCATCTCTTTCATTTTAGGACCGATTTTGCTTTCTTTCTATATGCACCACCACCGTCTCTGTTGCGATTTCTATACGGACGTCCATTTTGTTGAACATTGTAGGGTTTCATACGAGAGAGACACGAAGATCATGAACGCGGCGACCTTTATATTAGAGAGAGAGGATCACACCATTGGAAACATCATCCGCATGTAGGGTTTTCTGTTAATTGGAGAGCTTCTGGCGATTTTGATTCATTTCTTCGTTCTTGTTTATTGACGTGTGTGTGTACATGTATTTGTTCTGGCGTGAATAATCAGGCAACTTCACAGAGACGATAATGTGCTGTTTGCTGCGTATCAGCTTCCTCATCCTCTAAAGTACAGAATACTTGTCCGGGTTAGTACTTCGATTGTTTTTCCGTTGTGCAGGCGTTGTTCTATTGCATGAATGGTTAATTAAACAGCATTACGAGAATCTTTGCAATGCAATGTTGCTGTTTGGTAATGGCAGATTATACTCTGGAGGAGAACGGCTGATTGAATTTTTAATGTAACTAGCGTTAATTTATTCCCAGGTTTATCTGTTATTAGAGCTAGTTTAGAGATTGCTAAGTTATGCTTGGGGGCTAACATCATAGGGAGGGAGTCAAACTGGAATGAGAGTTTGCTCGACTGGCACGGTAGAGCCAAAAAATTTCATAGGAAATGGAAAATTTTACCACCAACTTTTTTATGATGAAAACTTGAAATCGTTAACTTCTGTTTCTATAGTTTTCTCAATATAATATGAACTGAGGGGAATTGTCCCTCCTTTACTCTTCACTTCACCCTTGCGACTGGAGTTCAAATAAACAATTTGCCCTGGAGGAAGTGAAATGTAGTTGAATGCTAATTTCCTTTTCCAAGCAGCGCAACATTTTCATTTTTCAATTTTCCCCACAGAAAAATCATGAACATTAAAGATTTTGAAATTCCTTGAGGATGGAGATATTTTGCAGATACTGATTGGTTTTGAATACTACAATTCTAGTAGCTTTATTATGGTCTGTTCTTGATTGAATTACAATCGACCATAAAGTTCTGATTGAAGTTCTTTTATCATTATTCTTTCAATATTACTGTTCTGCAGATACACACGGCTAGCCAGTCTTCACCGATGCAGGCATATAATCAGGCTATTAATGATTTGGACAAGGAGCTTGACCTTTTGAAATTTGAGTTCGAGGTAATGCTTTCACTCCCCCTTGACCATTTGTCTGCAATTTGGATGATGTGAGTATGATTTTGTTCTTTTTCTTATTCATTGTGATGGAATCCAGTCCTCTGCTTATTTTGCTCTTGAAGGCTTACACCTTTTTTTTTCCCCTTGATGCTAATCCTTGAATTTTGCAAAAAATGTATGGTCTTGTTAAATGTTCACATTACATTTAATATAGATGTCAGATCACTTATTTTTAAACTCTATAACCTAATGGGATAAATGTAGTTTACAGTTCAAGTTCATTTCAAATTATTTGAAGTTTTTGTTAAATGTACATAAGAATTTAATAGTTAAAGATTAAAAAATAAAGGGCAACAAGAAAAAGAAAAAAAAGGGGATTTTTATGCAAAATTTAAGGGATAAAAAGTTACAAGTCTAAAAATTTAACAGGGAAGAAAAAAAAAAAGATCATGACATTATTACCATTGTTATTTACATTCGTGAATTGCTTGATGCAGGGCGAACTTTCAAGATTCTCGAGGACCTATTAGGTTTGCCTTCAAAGAAAACCAGGTGGAAGTTCTGTTATCTGACCATTATAGAATTTCGATTCTTTGCCAACTCAATAAATTTGAGTTGGAAATGGAATGCCAAAAATAA

mRNA sequence

ATGAATGTCCCCGATCGCTACGAGCGCTTCGTCGTCCCCGAGGGCGCCAAGAAGGTTTCATACGAGAGAGACACGAAGATCATGAACGCGGCGACCTTTATATTAGAGAGAGAGGATCACACCATTGGAAACATCATCCGCATGCAACTTCACAGAGACGATAATGTGCTGTTTGCTGCGTATCAGCTTCCTCATCCTCTAAAGTACAGAATACTTGTCCGGATACACACGGCTAGCCAGTCTTCACCGATGCAGGCATATAATCAGGCTATTAATGATTTGGACAAGGAGCTTGACCTTTTGAAATTTGAGTTCGAGGTAATGCTTTCACTCCCCCTTGACCATTTGTCTGCAATTTGGATGATGGCGAACTTTCAAGATTCTCGAGGACCTATTAGGTTTGCCTTCAAAGAAAACCAGGTGGAAGTTCTGTTATCTGACCATTATAGAATTTCGATTCTTTGCCAACTCAATAAATTTGAGTTGGAAATGGAATGCCAAAAATAA

Coding sequence (CDS)

ATGAATGTCCCCGATCGCTACGAGCGCTTCGTCGTCCCCGAGGGCGCCAAGAAGGTTTCATACGAGAGAGACACGAAGATCATGAACGCGGCGACCTTTATATTAGAGAGAGAGGATCACACCATTGGAAACATCATCCGCATGCAACTTCACAGAGACGATAATGTGCTGTTTGCTGCGTATCAGCTTCCTCATCCTCTAAAGTACAGAATACTTGTCCGGATACACACGGCTAGCCAGTCTTCACCGATGCAGGCATATAATCAGGCTATTAATGATTTGGACAAGGAGCTTGACCTTTTGAAATTTGAGTTCGAGGTAATGCTTTCACTCCCCCTTGACCATTTGTCTGCAATTTGGATGATGGCGAACTTTCAAGATTCTCGAGGACCTATTAGGTTTGCCTTCAAAGAAAACCAGGTGGAAGTTCTGTTATCTGACCATTATAGAATTTCGATTCTTTGCCAACTCAATAAATTTGAGTTGGAAATGGAATGCCAAAAATAA

Protein sequence

MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAAYQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEFEVMLSLPLDHLSAIWMMANFQDSRGPIRFAFKENQVEVLLSDHYRISILCQLNKFELEMECQK
Homology
[Close]
BLAST of Lag0002765 vs. NCBI nr
Match: TYK10426.1 (DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. makuwa])

HSP 1 Score: 241.5 bits (615), Expect = 5.2e-60
Identity = 128/170 (75.29%), Postives = 136/170 (80.00%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNI+RMQLHRDDNVLFA 
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAG 60

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEFEVMLSLPLD---HLS 120
           YQLPHPLKYRILVRI TASQSSPMQAYNQAINDLDKELD+LK+E EVMLSLPLD    LS
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELEVMLSLPLDFFFSLS 120

Query: 121 AIWMMANFQDSRGPIRFAFKENQVEVLLSDHYRISILCQLNKFELEMECQ 168
           AI +   FQ SRGPI F  K+ + +             + N  EL MECQ
Sbjct: 121 AIGVRVRFQGSRGPISFPSKKTKGKF------------EFNNDELGMECQ 158

[Close]
BLAST of Lag0002765 vs. NCBI nr
Match: KAA0058617.1 (DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. makuwa])

HSP 1 Score: 219.9 bits (559), Expect = 1.6e-53
Identity = 108/114 (94.74%), Postives = 111/114 (97.37%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNI+RMQLHRDDNVLFA 
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAG 60

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEFEVMLSLPLD 115
           YQLPHPLKYRILVRI TASQSSPMQAYNQAINDLDKELD+LK+E EVMLSLPLD
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELEVMLSLPLD 114

[Close]
BLAST of Lag0002765 vs. NCBI nr
Match: XP_022150995.1 (DNA-directed RNA polymerases II, IV and V subunit 11-like [Momordica charantia])

HSP 1 Score: 209.9 bits (533), Expect = 1.7e-50
Identity = 102/110 (92.73%), Postives = 106/110 (96.36%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHT+GNI+RMQLHRDDNVLFAA
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTVGNILRMQLHRDDNVLFAA 60

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEFEVMLS 111
           YQLPHPLKYRIL+RIHTASQSSPMQAYNQA+NDLDKELD LKFE E  LS
Sbjct: 61  YQLPHPLKYRILIRIHTASQSSPMQAYNQAVNDLDKELDHLKFELESELS 110

[Close]
BLAST of Lag0002765 vs. NCBI nr
Match: XP_023004699.1 (DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 206.8 bits (525), Expect = 1.4e-49
Identity = 101/106 (95.28%), Postives = 104/106 (98.11%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFI+EREDHTIGNIIRMQLHRDDNVLFAA
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFIVEREDHTIGNIIRMQLHRDDNVLFAA 60

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEFE 107
           YQLPHPLKYRILVRI TASQSSPMQAYNQAINDLDKELD+LK+E E
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELE 106

[Close]
BLAST of Lag0002765 vs. NCBI nr
Match: KAG6593293.1 (DNA-directed RNA polymerases II, IV and V subunit 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 206.8 bits (525), Expect = 1.4e-49
Identity = 101/106 (95.28%), Postives = 104/106 (98.11%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFI+EREDHTIGNIIRMQLHRDDNVLFAA
Sbjct: 16  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFIVEREDHTIGNIIRMQLHRDDNVLFAA 75

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEFE 107
           YQLPHPLKYRILVRI TASQSSPMQAYNQAINDLDKELD+LK+E E
Sbjct: 76  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELE 121

[Close]
BLAST of Lag0002765 vs. ExPASy Swiss-Prot
Match: Q38859 (DNA-directed RNA polymerases II, IV and V subunit 11 OS=Arabidopsis thaliana OX=3702 GN=NRPB11 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.8e-47
Identity = 89/106 (83.96%), Postives = 99/106 (93.40%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MN P+RYERFVVPEG KKVSY+RDTKI+NAA+F +EREDHTIGNI+RMQLHRD+NVLFA 
Sbjct: 1   MNAPERYERFVVPEGTKKVSYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAG 60

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEFE 107
           YQLPHPLKY+I+VRIHT SQSSPMQAYNQAINDLDKELD LK +FE
Sbjct: 61  YQLPHPLKYKIIVRIHTTSQSSPMQAYNQAINDLDKELDYLKNQFE 106

[Close]
BLAST of Lag0002765 vs. ExPASy Swiss-Prot
Match: Q32P79 (DNA-directed RNA polymerase II subunit RPB11 OS=Bos taurus OX=9913 GN=POLR2J PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-24
Identity = 51/109 (46.79%), Postives = 75/109 (68.81%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MN P  +E F++ EG KK++  +DTK+ NA  F + +EDHT+GNII+ QL +D  VLFA 
Sbjct: 1   MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAG 60

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEFEVML 110
           Y++PHPL+++I++R+ T    SP +A+  AI DL  EL LL+  F V +
Sbjct: 61  YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAI 109

[Close]
BLAST of Lag0002765 vs. ExPASy Swiss-Prot
Match: P52435 (DNA-directed RNA polymerase II subunit RPB11-a OS=Homo sapiens OX=9606 GN=POLR2J PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-24
Identity = 51/109 (46.79%), Postives = 75/109 (68.81%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MN P  +E F++ EG KK++  +DTK+ NA  F + +EDHT+GNII+ QL +D  VLFA 
Sbjct: 1   MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAG 60

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEFEVML 110
           Y++PHPL+++I++R+ T    SP +A+  AI DL  EL LL+  F V +
Sbjct: 61  YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAI 109

[Close]
BLAST of Lag0002765 vs. ExPASy Swiss-Prot
Match: Q86JJ5 (DNA-directed RNA polymerase II subunit rpb11 OS=Dictyostelium discoideum OX=44689 GN=polr2j PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.1e-24
Identity = 50/105 (47.62%), Postives = 77/105 (73.33%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MN PDR+E FV+P+GAKKV+  RDTKI NA+ F + +EDHTIGN+IRMQL  D +++FA 
Sbjct: 1   MNAPDRFELFVLPDGAKKVTMVRDTKIPNASMFTILKEDHTIGNLIRMQLIADQDIIFAG 60

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEF 106
           Y++PHPL++ + +RI T + ++P++   +++  L +E   L+  F
Sbjct: 61  YRMPHPLEHNVNIRIQTNNNTNPLECVQKSMECLSREFTSLENSF 105

[Close]
BLAST of Lag0002765 vs. ExPASy Swiss-Prot
Match: O08740 (DNA-directed RNA polymerase II subunit RPB11 OS=Mus musculus OX=10090 GN=Polr2j PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.7e-24
Identity = 50/105 (47.62%), Postives = 73/105 (69.52%), Query Frame = 0 

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNIIRMQLHRDDNVLFAA 60
           MN P  +E F++ EG KK++  +DTK+ NA  F + +EDHT+GNII+ QL +D  VLFA 
Sbjct: 1   MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAG 60

Query: 61  YQLPHPLKYRILVRIHTASQSSPMQAYNQAINDLDKELDLLKFEF 106
           Y++PHPL+++I++R+ T    SP +A+  AI DL  EL LL+  F
Sbjct: 61  YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERF 105

The following BLAST results are available for this feature:
BLAST of Lag0002765 vs. NCBI nr
Match NameE-valueIdentityDescription
TYK10426.15.2e-6075.29DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. mak... [more]
KAA0058617.11.6e-5394.74DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. mak... [more]
XP_022150995.11.7e-5092.73DNA-directed RNA polymerases II, IV and V subunit 11-like [Momordica charantia][more]
XP_023004699.11.4e-4995.28DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1 [Cucurbita ... [more]
KAG6593293.11.4e-4995.28DNA-directed RNA polymerases II, IV and V subunit 11, partial [Cucurbita argyros... [more]
BLAST of Lag0002765 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q388591.8e-4783.96DNA-directed RNA polymerases II, IV and V subunit 11 OS=Arabidopsis thaliana OX=... [more]
Q32P791.3e-2446.79DNA-directed RNA polymerase II subunit RPB11 OS=Bos taurus OX=9913 GN=POLR2J PE=... [more]
P524351.3e-2446.79DNA-directed RNA polymerase II subunit RPB11-a OS=Homo sapiens OX=9606 GN=POLR2J... [more]
Q86JJ52.1e-2447.62DNA-directed RNA polymerase II subunit rpb11 OS=Dictyostelium discoideum OX=4468... [more]
O087403.7e-2447.62DNA-directed RNA polymerase II subunit RPB11 OS=Mus musculus OX=10090 GN=Polr2j ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 84..104
NoneNo IPR availablePANTHERPTHR13946:SF16DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11coord: 1..107
NoneNo IPR availablePANTHERPTHR13946DNA-DIRECTED RNA POLYMERASE I,II,IIIcoord: 1..107
IPR036603RNA polymerase, RBP11-like subunitGENE3D3.30.1360.10coord: 1..111
e-value: 2.9E-45
score: 154.7
IPR036603RNA polymerase, RBP11-like subunitSUPERFAMILY55257RBP11-like subunits of RNA polymerasecoord: 1..106
IPR009025DNA-directed RNA polymerase, RBP11-like dimerisation domainPFAMPF13656RNA_pol_L_2coord: 31..101
e-value: 2.3E-22
score: 78.6
IPR008193DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved sitePROSITEPS01154RNA_POL_L_13KDcoord: 35..66
IPR037685RNA polymerase RBP11CDDcd06926RNAP_II_RPB11coord: 13..105
e-value: 2.84765E-46
score: 144.657

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002765.1Lag0002765.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006366 transcription by RNA polymerase II
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005665 RNA polymerase II, core complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0001055 RNA polymerase II activity
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity