Lag0002753 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002753 vs. NCBI nr
Match: XP_031745107.1 (putative disease resistance protein RGA4 [Cucumis sativus])

HSP 1 Score: 615.1 bits (1585), Expect = 7.2e-172
Identity = 419/1043 (40.17%), Postives = 510/1043 (48.90%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRH 60
            MAE +LF +AGEILMKL+S A +RLGML G + DLNKL + +S I+ VL DAE   TK H
Sbjct: 1    MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 61   SLKDCLEKLGEVLYDADDVLDEFSCEALHREVMT-GGKNAKQLNKIF------------- 120
             L++ L KL E LYDA+DVLDE S EAL RE+MT   KNAKQ+   F             
Sbjct: 61   LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120

Query: 121  --------------------------EPRIQHDSSHQIGTKWETHSSLCGEEVIGRDDDK 180
                                      E R Q+ S  +I    ET SS   EEVIGRDDD 
Sbjct: 121  RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180

Query: 181  MHLKSLLLD--ENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSEQF 240
              +K  LLD   NV  NVSFIAI GMGGIGKTTLAK LYND EVS  F+LK+WVWVS+QF
Sbjct: 181  KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240

Query: 241  DEKIIVKKIIESATDSQPNAQ--------------------------------------- 300
            + +++ +K+IESAT + P+ +                                       
Sbjct: 241  EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300

Query: 301  ------------------------------------EMDPWILYN--------------- 360
                                                E + W+L++               
Sbjct: 301  LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360

Query: 361  ----------------------------------VSFK---------------------- 420
                                              +SFK                      
Sbjct: 361  IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420

Query: 421  ------------------------------------------------------------ 480
                                                                        
Sbjct: 421  NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480

Query: 481  -------SDEYNFDDRVHMHDVMRELSKKVAGMKLYVHGNPNNDYDVSEQTRHISFD-KI 540
                   SDE N +D V MHDVM E  +KVAG KLYV GNPNNDY VSEQT HISFD  I
Sbjct: 481  RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540

Query: 541  ESWQAVLSKLRKAKGLRTFLLLHSYYEEWNE----------------------------- 600
            +SWQ VLSKL KAKGLRT LLL   YE+ N+                             
Sbjct: 541  QSWQDVLSKLCKAKGLRTILLLFRPYEKMNKIDKAILDELFSSFPRLRVLDLHFSQISVV 600

Query: 601  -------------------------------------LTTCLSLKELPRNTSNLINLRHL 660
                                                 LT C  LKELPR+  NL+NLRHL
Sbjct: 601  PKSIKKLRHLRYLDLSENDMELIPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLRHL 660

Query: 661  DFS-SKKIRHMPKGMEKLTCLQTISLFELDCKRFSKLSELNKFNNLSAKSLRIIGLEQLR 696
             F    ++    +GMEKLTCLQTISLF  DCK+ +KL ELN  + L+ + L+IIGLE+LR
Sbjct: 661  TFEPCMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSYLTGE-LKIIGLEKLR 720

BLAST of Lag0002753 vs. NCBI nr
Match: KAE8646395.1 (hypothetical protein Csa_016171 [Cucumis sativus])

HSP 1 Score: 615.1 bits (1585), Expect = 7.2e-172
Identity = 419/1043 (40.17%), Postives = 510/1043 (48.90%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRH 60
            MAE +LF +AGEILMKL+S A +RLGML G + DLNKL + +S I+ VL DAE   TK H
Sbjct: 1    MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 61   SLKDCLEKLGEVLYDADDVLDEFSCEALHREVMT-GGKNAKQLNKIF------------- 120
             L++ L KL E LYDA+DVLDE S EAL RE+MT   KNAKQ+   F             
Sbjct: 61   LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120

Query: 121  --------------------------EPRIQHDSSHQIGTKWETHSSLCGEEVIGRDDDK 180
                                      E R Q+ S  +I    ET SS   EEVIGRDDD 
Sbjct: 121  RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180

Query: 181  MHLKSLLLD--ENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSEQF 240
              +K  LLD   NV  NVSFIAI GMGGIGKTTLAK LYND EVS  F+LK+WVWVS+QF
Sbjct: 181  KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240

Query: 241  DEKIIVKKIIESATDSQPNAQ--------------------------------------- 300
            + +++ +K+IESAT + P+ +                                       
Sbjct: 241  EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300

Query: 301  ------------------------------------EMDPWILYN--------------- 360
                                                E + W+L++               
Sbjct: 301  LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360

Query: 361  ----------------------------------VSFK---------------------- 420
                                              +SFK                      
Sbjct: 361  IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420

Query: 421  ------------------------------------------------------------ 480
                                                                        
Sbjct: 421  NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480

Query: 481  -------SDEYNFDDRVHMHDVMRELSKKVAGMKLYVHGNPNNDYDVSEQTRHISFD-KI 540
                   SDE N +D V MHDVM E  +KVAG KLYV GNPNNDY VSEQT HISFD  I
Sbjct: 481  RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540

Query: 541  ESWQAVLSKLRKAKGLRTFLLLHSYYEEWNE----------------------------- 600
            +SWQ VLSKL KAKGLRT LLL   YE+ N+                             
Sbjct: 541  QSWQDVLSKLCKAKGLRTILLLFRPYEKMNKIDKAILDELFSSFPRLRVLDLHFSQISVV 600

Query: 601  -------------------------------------LTTCLSLKELPRNTSNLINLRHL 660
                                                 LT C  LKELPR+  NL+NLRHL
Sbjct: 601  PKSIKKLRHLRYLDLSENDMELIPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLRHL 660

Query: 661  DFS-SKKIRHMPKGMEKLTCLQTISLFELDCKRFSKLSELNKFNNLSAKSLRIIGLEQLR 696
             F    ++    +GMEKLTCLQTISLF  DCK+ +KL ELN  + L+ + L+IIGLE+LR
Sbjct: 661  TFEPCMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSYLTGE-LKIIGLEKLR 720

BLAST of Lag0002753 vs. NCBI nr
Match: XP_008461332.2 (PREDICTED: disease resistance protein RGA2 [Cucumis melo])

HSP 1 Score: 602.8 bits (1553), Expect = 3.7e-168
Identity = 417/1054 (39.56%), Postives = 516/1054 (48.96%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRH 60
            MAEG+L  +AGEIL KL S  S   GML G +DDL++LKS +S IQ+ L DA+  PT   
Sbjct: 255  MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNLPTGLD 314

Query: 61   SLKDCLEKLGEVLYDADDVLDEFSCEALHREVMTGGKNAKQLNKIFEPRIQHDSSHQIGT 120
            S+   LEKL  VL+DA+D++DEF  E  HREVMT  K+AK++   F    Q   ++++  
Sbjct: 315  SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 374

Query: 121  K------------------W------------------------ETHSSLCGEEVIGRDD 180
            +                  W                        ETHSSL  E VIGRDD
Sbjct: 375  RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRMRMSLSRETHSSLNKERVIGRDD 434

Query: 181  DKMHLKSLLL--DENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSE 240
            DK +LKSLLL  D ++KDNVS IAIVGMGGIGKTTLAK LYND +VS  FE+KMW+WVS+
Sbjct: 435  DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSKHFEIKMWIWVSK 494

Query: 241  QFDEKIIVKKIIESATDSQPNAQEMD---------------------------------- 300
            QF EKI+VKKI+ESAT   P  QE+D                                  
Sbjct: 495  QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 554

Query: 301  -----------------------------------------PWILY-NVSFK-------- 360
                                                      W L+  V+F+        
Sbjct: 555  DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDRSWSLFREVAFEGNLPNPIN 614

Query: 361  ------------------------------------------------------------ 420
                                                                        
Sbjct: 615  PKLELMGKEISSRCGGVPLAIRHMGRFLCSKRCEEEWMSSLSDKLLEVTQQNNDVASILE 674

Query: 421  ------------------------------------------------------------ 480
                                                                        
Sbjct: 675  LSYNDLPLHLKQCFAYSSLFPKVHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFDE 734

Query: 481  ----------SDEYNFDDRVHMHDVMRELSKKVAGMKLYVHGNPNNDYDVSEQTRHISFD 540
                      SDE NFDD V+MHDVM EL++KVA  KLYV G+ NN+Y VSEQT H SFD
Sbjct: 735  LCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLYVCGDINNNYVVSEQTLHFSFD 794

Query: 541  -KIESWQAVLSKLRKAKGLRTFLLLHSYYEEWNE-------------------------- 600
             KI+SWQ VLSKL KAKGLRTF+ LHS YEE NE                          
Sbjct: 795  YKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKNEVNEANLDELFSSFPRLRVLLLGGSNI 854

Query: 601  ----------------------------------------LTTCLSLKELPRNTSNLINL 660
                                                    LT C +LKE+PR+ + L+NL
Sbjct: 855  HVLPNSIKKLKLLRYLDLSENNMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKLVNL 914

Query: 661  RHLDF-SSKKIRHM-PKGMEKLTCLQTISLFELDCKRFSKLSELNKFNNLSAKSLRIIGL 701
            RHLD     +I HM P+GMEKL+CLQTISLF  DC + +KL ELN+ NNL+ + L+I GL
Sbjct: 915  RHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKTNKLRELNELNNLTGE-LKITGL 974

BLAST of Lag0002753 vs. NCBI nr
Match: KAA0058606.1 (disease resistance protein RGA2 [Cucumis melo var. makuwa])

HSP 1 Score: 602.4 bits (1552), Expect = 4.8e-168
Identity = 417/1055 (39.53%), Postives = 516/1055 (48.91%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRH 60
            MAEG+L  +AGEIL KL S  S   GML G +DDL++LKS +S IQ+ L DA+  PT   
Sbjct: 1    MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNWPTGLD 60

Query: 61   SLKDCLEKLGEVLYDADDVLDEFSCEALHREVMTGGKNAKQLNKIFEPRIQHDSSHQIGT 120
            S+   LEKL  VL+DA+D++DEF  E  HREVMT  K+AK++   F    Q   ++++  
Sbjct: 61   SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 120

Query: 121  K------------------W------------------------ETHSSLCGEEVIGRDD 180
            +                  W                        ETHSSL  E VIGRDD
Sbjct: 121  RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRMRMSSSRETHSSLNKERVIGRDD 180

Query: 181  DKMHLKSLLL--DENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSE 240
            DK +LKSLLL  D ++KDNVS IAIVGMGGIGKTTLAK LYND +VS+ FE+KMW+WVS+
Sbjct: 181  DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSEHFEIKMWIWVSK 240

Query: 241  QFDEKIIVKKIIESATDSQPNAQEMD---------------------------------- 300
            QF EKI+VKKI+ESAT   P  QE+D                                  
Sbjct: 241  QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 300

Query: 301  -----------------------------------------PWILY-------------- 360
                                                      W L+              
Sbjct: 301  DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDSSWSLFREVAFEENLPKPIN 360

Query: 361  ---------------------------------------NVSFK---------------- 420
                                                   ++S+K                
Sbjct: 361  PKLELMGKEISSICGGVPLAIRHMGRFLCSKRCEEEWMSSLSWKKHLEVAQQNNDVASIL 420

Query: 421  ------------------------------------------------------------ 480
                                                                        
Sbjct: 421  ELSYNDLPLHLKQCFAYSSLFPKGHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFD 480

Query: 481  -----------SDEYNFDDRVHMHDVMRELSKKVAGMKLYVHGNPNNDYDVSEQTRHISF 540
                       SDE NFDD V+MHDVM EL++KVA  KL+V G+ NN+Y VSEQT H S 
Sbjct: 481  ELCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLFVCGDINNNYVVSEQTLHFSL 540

Query: 541  D-KIESWQAVLSKLRKAKGLRTFLLLHSYYEEWNE------------------------- 600
            D KI+SWQ VLSKL KAKGLRTF+ LHS YEE NE                         
Sbjct: 541  DYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKNEVNEANLDELFSSFPRLRVLLLGGSN 600

Query: 601  -----------------------------------------LTTCLSLKELPRNTSNLIN 660
                                                     LT C +LKE+PR+ + L+N
Sbjct: 601  IHVLPNSIKKLKLLRYLDLSENNMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKLVN 660

Query: 661  LRHLDF-SSKKIRHM-PKGMEKLTCLQTISLFELDCKRFSKLSELNKFNNLSAKSLRIIG 701
            LRHLD     +I HM P+GMEKL+CLQTISLF  DC + +KL ELN+ NNL+ + L+I G
Sbjct: 661  LRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKTNKLRELNELNNLTGE-LKITG 720

BLAST of Lag0002753 vs. NCBI nr
Match: XP_038897566.1 (putative disease resistance protein RGA4 [Benincasa hispida])

HSP 1 Score: 586.6 bits (1511), Expect = 2.8e-163
Identity = 420/1043 (40.27%), Postives = 506/1043 (48.51%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRH 60
            MAE +LF IAGEILMKL+S A +RLGML G +D+LNKL + +S I+ VL DAE   TK H
Sbjct: 1    MAEAILFDIAGEILMKLSSQAFQRLGMLFGLKDNLNKLTATVSTIKDVLLDAEGRQTKGH 60

Query: 61   SLKDCLEKLGEVLYDADDVLDEFSCEALHREVMTGGKNAKQLNKIF-------------- 120
             L++ L+KL E LYDA+DVLDE S EALHREVMT  KNAKQ+   F              
Sbjct: 61   LLENWLQKLEEALYDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQIAFNYSMAR 120

Query: 121  -------------------------EPRIQHDSSHQIGTKWETHSSLCGEEVIGRDDDKM 180
                                     E + ++ S  QI T  E+ S   GEEVIGRDDDK 
Sbjct: 121  QIKKIWERLDAIDAEKKQFHLHENCESKARYGSFDQIMTGRESWSFSNGEEVIGRDDDKK 180

Query: 181  HLKSLLLD--ENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSEQFD 240
             +K LLLD   NV  NVS +AIVGMGGIGKTTLAK LYND ++S  FELK+W+WVS+QF+
Sbjct: 181  KIKDLLLDVNMNVTHNVSIVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWIWVSDQFE 240

Query: 241  EKIIVKKIIESATDSQPNAQEM---------------------DPW-------------- 300
             K +VKKIIESAT S P+   M                     D W              
Sbjct: 241  IKAVVKKIIESATKSNPHVMGMEALQAELQKVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300

Query: 301  ------------------------------------------------------------ 360
                                                                        
Sbjct: 301  MSGAKGSKILITKRDRKVAAEIESMTSLFSLEGLPESKSWSLFSKVAFKEGKELENPNLI 360

Query: 361  ----------------------ILYN-------VSFKSDEY------------------- 420
                                  +LY+       +SFK +E                    
Sbjct: 361  QTGKEILMKCGGVPLVIRHIGRLLYSRTTEEEWMSFKDNELLEIIQQDNDMTSVLKLSYN 420

Query: 421  ------------------------------------------------------------ 480
                                                                        
Sbjct: 421  HLPPNLKQCFAYLSLFPKRKSLKINDLIRQWMALGFIETSNGSKSMEETGKGYFKELCWR 480

Query: 481  ----------NFDDRVHMHDVMRELSKKVAGMKLYVHGNPNNDYDVSEQTRHISFD-KIE 540
                      NFD+ VHMHDVMR+L+  VAG K YV  N N DY  SE+TRH+SFD KI+
Sbjct: 481  FFLENSMNECNFDNDVHMHDVMRDLATNVAGKK-YVRENINCDYAFSEKTRHVSFDCKIK 540

Query: 541  SWQAVLSKLRKAKGLRTFLLLHSYYE-----EWNE------------------------- 600
            SW  VLSKL KAKGLRTF L  S +E     E NE                         
Sbjct: 541  SWSDVLSKLHKAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSPM 600

Query: 601  --------------------------------------LTTCLSLKELPRNTSNLINLRH 660
                                                  L  C  LKELPR+TSNL+NLRH
Sbjct: 601  VPNSIRRLRHLRYLDLSENNMESLPNSITELRNLQTLNLMYCGDLKELPRDTSNLVNLRH 660

Query: 661  LDFSSKKIRHMPKGMEKLTCLQTISLFELDCKRFSKLSELNKFNNLSAKSLRIIGLEQLR 696
            LDF++  + HMP+GM KL+CLQT+S F LDCKR ++LSEL+   +L+   LRIIGLEQLR
Sbjct: 661  LDFNTSILTHMPEGMGKLSCLQTLSYFVLDCKRSNQLSELDVLIHLNG-YLRIIGLEQLR 720

BLAST of Lag0002753 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 3.5e-52
Identity = 255/1002 (25.45%), Postives = 391/1002 (39.02%), Query Frame = 0

Query: 13  ILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRHSLKDCLEKLGEV 72
           +L  LT      LG++ GFE +  KL S  S IQAVL DA+E   K  ++K+ L+KL   
Sbjct: 9   LLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVA 68

Query: 73  LYDADDVLDEFSCEALH-REVMTG-------------GKNAKQ----LNKIFEPRIQHDS 132
            Y+ DD+LD+   EA   ++ + G             GK  K+    L+ I E R     
Sbjct: 69  AYEVDDILDDCKTEAARFKQAVLGRYHPRTITFCYKVGKRMKEMMEKLDAIAEERRNFHL 128

Query: 133 SHQI----GTKWETHSSLCGEEVIGRDDDKMHLKSLLLDE-NVKDNVSFIAIVGMGGIGK 192
             +I      + +T   L   +V GR+ ++  +  +L++  +  + V  + I+GMGG+GK
Sbjct: 129 DERIIERQAARRQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLGK 188

Query: 193 TTLAKFLYNDSEVSDCFELKMWVWVSEQFDEKIIVKKIIESATDS----------QPNAQ 252
           TTLA+ ++ND  +++ F LK+WV VS+ FDEK ++K I+ES              Q   Q
Sbjct: 189 TTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAPLQKKLQ 248

Query: 253 EM-----------DPW---------------------------------------ILYNV 312
           E+           D W                                        LY +
Sbjct: 249 ELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLEKIGSIMGTLQLYQL 308

Query: 313 SFKSDE---YNFDDRVHMH---------DVMRELSKKVAGMKL----------------- 372
           S  S E     F  R   H         ++ +E+ KK  G+ L                 
Sbjct: 309 SNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKREESE 368

Query: 373 -------YVHGNPNNDYDVSEQTR----HISFD---------------KIES------WQ 432
                   +   P ++  V    R    H+  D               KIE       W 
Sbjct: 369 WEHVRDSEIWNLPQDENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEYLIALWM 428

Query: 433 A---VLSK-----------------LR------KAKGLRTFLLLHSYYEE---------- 492
           A   +LSK                 LR      + K  +T+  +H    +          
Sbjct: 429 AHSFLLSKGNMELEDVGNEVWNELYLRSFFQEIEVKSGKTYFKMHDLIHDLATSMFSASA 488

Query: 493 ------------------------------WNELTTCLS-------------------LK 552
                                         ++E+ +  S                    +
Sbjct: 489 SSRSIRQINVKDDEDMMFIVTNYKDMMSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFE 548

Query: 553 ELPRNTSNLINLRHLDFSSKKIRHMPKGMEKLTCLQTISLF---ELDC--KRFSKLS--- 612
           +LP +  +L++LR+LD S  KI  +PK + KL  LQT+ L+    L C  K+ SKL    
Sbjct: 549 QLPSSVGDLVHLRYLDLSGNKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLR 608

Query: 613 ------------------------------------ELNKFNNLSAK-SLRIIGLEQLRS 672
                                               +L +  NL+ + ++ I  LE++++
Sbjct: 609 NLVLDHCPLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNLNLRGAISITHLERVKN 668

Query: 673 TSFEVSSVNLKEKTNFRRLILEWE----LSADECELVK-------------LRYSGVGVP 700
              E    NL  K N   L + W+      ++E ++++             + + G  +P
Sbjct: 669 -DMEAKEANLSAKANLHSLSMSWDRPNRYESEEVKVLEALKPHPNLKYLEIIDFCGFCLP 728

BLAST of Lag0002753 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.9e-50
Identity = 249/985 (25.28%), Postives = 381/985 (38.68%), Query Frame = 0

Query: 13  ILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRHSLKDCLEKLGEV 72
           +L  LTS     L +L GF+D+  +L S  S IQAVL DA+E       L++ L+KL   
Sbjct: 9   LLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAA 68

Query: 73  LYDADDVLDEFSCEAL------------------HREVMTGGKNAKQLNKIFEPRIQHDS 132
            Y+ DD+LDE+  +A                   H+      +  K+L  I E R     
Sbjct: 69  TYEVDDILDEYKTKATRFSQSEYGRYHPKVIPFRHKVGKRMDQVMKKLKAIAEERKNFHL 128

Query: 133 SHQI----GTKWETHSSLCGEEVIGRDDDKMHLKSLLLDENVKD--NVSFIAIVGMGGIG 192
             +I      + ET S L   +V GRD +K  +  +L++ NV D  ++S + I+GMGG+G
Sbjct: 129 HEKIVERQAVRRETGSVLTEPQVYGRDKEKDEIVKILIN-NVSDAQHLSVLPILGMGGLG 188

Query: 193 KTTLAKFLYNDSEVSDCFELKMWVWVSEQFDEKIIVKKIIESATDSQPNAQEMD------ 252
           KTTLA+ ++ND  V++ F  K+W+ VSE FDEK ++K I+ES  + +P   EMD      
Sbjct: 189 KTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVES-IEGRPLLGEMDLAPLQK 248

Query: 253 -----------------------------------------------------------P 312
                                                                      P
Sbjct: 249 KLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGASGASVLTTTRLEKVGSIMGTLQP 308

Query: 313 WILYNVSFKSDEYNFDDRVHMHD---------VMRELSKKVAGMKL-------------- 372
           + L N+S +     F  R   H          + +E+ KK  G+ L              
Sbjct: 309 YELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKRE 368

Query: 373 -----YVHGNP--NNDYDVSE--QTRHISFDKIE-------SWQAVL---SKLRKAKGLR 432
                +V  +P  N   D S       +S+ ++        ++ AV    +K+ K K + 
Sbjct: 369 ERAWEHVRDSPIWNLPQDESSILPALRLSYHQLPLDLKQCFAYCAVFPKDAKMEKEKLIS 428

Query: 433 TF------------------------LLLHSYYEE---------------WNELTTCL-- 492
            +                        L L S+++E                ++L T L  
Sbjct: 429 LWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQEIEVKDGKTYFKMHDLIHDLATSLFS 488

Query: 493 --------------------------------------------------SLKELPRNTS 552
                                                             +  +LP +  
Sbjct: 489 ANTSSSNIREINKHSYTHMMSIGFAEVVFFYTLPPLEKFISLRVLNLGDSTFNKLPSSIG 548

Query: 553 NLINLRHLDFSSKKIRHMPKGMEK------------------------------------ 612
           +L++LR+L+     +R +PK + K                                    
Sbjct: 549 DLVHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGS 608

Query: 613 ------------LTCLQTISLFELDCKRFSKLSELNKFNNLSAKSLRIIGLEQLRSTSFE 672
                       LTCL+T+  F +  K+  +L EL   N     S++I  LE++++   +
Sbjct: 609 QSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNLNLYG--SIKISHLERVKNDK-D 668

Query: 673 VSSVNLKEKTNFRRLILEW----------------ELSADECELVKLR---YSGVGVPNW 700
               NL  K N   L + W                E       L  L+   + G+ +P W
Sbjct: 669 AKEANLSAKGNLHSLSMSWNNFGPHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEW 728

BLAST of Lag0002753 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 6.2e-49
Identity = 246/988 (24.90%), Postives = 379/988 (38.36%), Query Frame = 0

Query: 13  ILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRHSLKDCLEKLGEV 72
           +L  LTS     L +L GF+D+  +L S  S IQAVL DA+E       L++ L+KL   
Sbjct: 9   VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68

Query: 73  LYDADDVLDEFSCEAL------------------HREVMTGGKNAKQLNKIFEPR----I 132
            Y+ DD+LDE+  +A                   H+      +  K+LN I E R    +
Sbjct: 69  TYEVDDILDEYKTKATRFLQSEYGRYHPKVIPFRHKVGKRMDQVMKKLNAIAEERKKFHL 128

Query: 133 QHDSSHQIGTKWETHSSLCGEEVIGRDDDKMHLKSLLLD-ENVKDNVSFIAIVGMGGIGK 192
           Q     +     ET S L   +V GRD +K  +  +L++  +    +S + I+GMGG+GK
Sbjct: 129 QEKIIERQAATRETGSVLTEPQVYGRDKEKDEIVKILINTASDAQKLSVLPILGMGGLGK 188

Query: 193 TTLAKFLYNDSEVSDCFELKMWVWVSEQFDEKIIVKKIIESATDS----------QPNAQ 252
           TTL++ ++ND  V++ F  K+W+ +S+ F+EK ++K I+ES              Q   Q
Sbjct: 189 TTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKSLSDMDLAPLQKKLQ 248

Query: 253 E-----------------------------------------------------MDPWIL 312
           E                                                     + P+ L
Sbjct: 249 ELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVLTTTRLEKVGSIMGTLQPYEL 308

Query: 313 YNVSFKSDEYNFDDRVHMHD---------VMRELSKKVAGMKL----------------- 372
            N+S +   + F  R   H          + +E+ KK  G+ L                 
Sbjct: 309 SNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKREERE 368

Query: 373 --YVHGNP--NNDYDVSE-------QTRHISFDKIES--WQAVLSKLRK--AKGLRTFLL 432
             +V  +P  N   D S           H+  D  +   + AV  K  K   + L  F +
Sbjct: 369 WEHVRDSPIWNLPQDESSILPALRLSYHHLPLDLRQCFVYCAVFPKDTKMAKENLIAFWM 428

Query: 433 LHSYY-------------EEWNE---------------------------LTTCL----- 492
            H +              E WNE                           L T L     
Sbjct: 429 AHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEVESGKTYFKMHDLIHDLATSLFSANT 488

Query: 493 -----------------------------------------------SLKELPRNTSNLI 552
                                                          +L +LP +  +L+
Sbjct: 489 SSSNIREINANYDGYMMSIGFAEVVSSYSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLV 548

Query: 553 NLRHLDFSSK-KIRHMPKGMEK-------------------------------------- 612
           +LR+LD S   +IR++PK + K                                      
Sbjct: 549 HLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSL 608

Query: 613 ---------LTCLQTISLFELDCKRFSKLSELNKFNNLSAKSLRIIGLEQLRSTSFEVSS 672
                    LTCL+++S F +  ++  +L EL   N     S+ I  L++++  + +   
Sbjct: 609 TSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNLNLYG--SISITKLDRVKKDT-DAKE 668

Query: 673 VNLKEKTNFRRLILEWEL------SADECELVK----LRY------SGVGVPNWVSFSLL 700
            NL  K N   L L W+L       ++  E +K    L+Y       G+ +P+W++ S+L
Sbjct: 669 ANLSAKANLHSLCLSWDLDGKHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVL 728

BLAST of Lag0002753 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 9.8e-47
Identity = 238/998 (23.85%), Postives = 388/998 (38.88%), Query Frame = 0

Query: 13  ILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRHSLKDCLEKLGEV 72
           +L  LTS    +L ++ GFE +  KL S  S IQAVL DA+E   K  ++++ L+KL   
Sbjct: 9   LLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSA 68

Query: 73  LYDADDVLDEFSCEALHREVMTGG------------------KNAKQLNKIFEPRIQHDS 132
            Y+ DD+L E   EA+  E    G                  +  ++L+ I E R +   
Sbjct: 69  AYEVDDILGECKNEAIRFEQSRLGFYHPGIINFRHKIGRRMKEIMEKLDAISEERRKFHF 128

Query: 133 SHQIGTKW------ETHSSLCGEEVIGRDDDKMHLKSLLLDE-NVKDNVSFIAIVGMGGI 192
             +I  +       ET   L   +V GRD ++  +  +L++  NV + +    I+GMGG+
Sbjct: 129 LEKITERQAAAATRETGFVLTEPKVYGRDKEEDEIVKILINNVNVAEELPVFPIIGMGGL 188

Query: 193 GKTTLAKFLYNDSEVSDCFELKMWVWVSEQFDEKIIVKKIIESATDSQPNAQE------- 252
           GKTTLA+ ++ND  V+  F  K+WV VS+ FDEK ++K II +   S P+ ++       
Sbjct: 189 GKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQKK 248

Query: 253 --------------------------------------------------------MDPW 312
                                                                   + P+
Sbjct: 249 LQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGASILATTRLEKVGSIMGTLQPY 308

Query: 313 ILYNVSFKSDEYNFDDRV---------HMHDVMRELSKKVAGMKL--------------- 372
            L N+S       F  R          ++  + +E+ KK  G+ L               
Sbjct: 309 HLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKREE 368

Query: 373 ----YVHGN-----PNNDYDVSEQTR----HISFD--KIESWQAVLSKLRK--AKGLRTF 432
               +V  N     P ++  +    R    H+  D  +  ++ AV  K  K   + L T 
Sbjct: 369 SEWEHVRDNEIWSLPQDESSILPALRLSYHHLPLDLRQCFAYCAVFPKDTKMIKENLITL 428

Query: 433 LLLHSYY-------------EEWNEL----------------------------TTCLS- 492
            + H +              E WNEL                            T+  S 
Sbjct: 429 WMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEAKSGNTYFKIHDLIHDLATSLFSA 488

Query: 493 --------------------------------------------------LKELPRNTSN 552
                                                             L++LP +  +
Sbjct: 489 SASCGNIREINVKDYKHTVSIGFAAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSSIGD 548

Query: 553 LINLRHLDFSSKKIRHMPKGMEKLTCLQTISL---FELDC--KRFSKLS----------- 612
           L++LR+LD S    R +P+ + KL  LQT+ +   + L+C  K+ SKLS           
Sbjct: 549 LLHLRYLDLSCNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCP 608

Query: 613 ----------------------------ELNKFNNLS-AKSLRIIGLEQLRSTSFEVSSV 672
                                       +L +  NL+   S+ I  LE++++ +   +  
Sbjct: 609 LTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNLNLCGSISITHLERVKNDT--DAEA 668

Query: 673 NLKEKTNFRRLILEWE------LSADECELVK-------------LRYSGVGVPNWVSFS 700
           NL  K N + L + W+        + E ++++             + + G   P+W++ S
Sbjct: 669 NLSAKANLQSLSMSWDNDGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHS 728

BLAST of Lag0002753 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.5e-23
Identity = 108/424 (25.47%), Postives = 179/424 (42.22%), Query Frame = 0

Query: 316  LTTCLSLKELPRNTSNLINLRHLDFSSKKIRHMPKGMEKLTCLQTISLFELDCKRFSKLS 375
            L+ C SLKELP + SNLINLR+LD    K+R MP+   +L  LQT++ F +     S++S
Sbjct: 632  LSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRIS 691

Query: 376  ELNKFNNLSAKSLRIIGLEQLRSTSFEVSSVNLKEKTNFRRLILEWELSADECE------ 435
            EL   ++L  K L+I+ L+++   + + +  NL  K + R +   W   +   E      
Sbjct: 692  ELGGLHDLHGK-LKIVELQRVVDVA-DAAEANLNSKKHLREIDFVWRTGSSSSENNTNPH 751

Query: 436  --------------------LVKLRYSGVGVPNWVSF-SLLKLTKITIANCHRLQHLPQF 495
                                L   RY G   P+W+S  S  ++  I +  C     LP  
Sbjct: 752  RTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSL 811

Query: 496  SHLEALEFLYLKDLRCLKFIDTNDPYSSSVF-------FPSLKKLYLEDMPNLKGWWELG 555
              L  L+ L++  +  L+ I     +S           F SL+ L  +++P+ + W ++ 
Sbjct: 812  GQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVR 871

Query: 556  ESRAGETENKCSPFIPELECLHIRHCPKLT-SMPPFIPTISVILCDVGVQLLSTIGPLSH 615
             +R            P L+ L I  CP+LT ++P F+P++  +                H
Sbjct: 872  VTR--------GDLFPSLKKLFILRCPELTGTLPTFLPSLISL----------------H 931

Query: 616  VEHLKLAEMKDLKYLPDEFHQNLLSLGTSTISSPIRPGVLQIEECPNLMSLPEWIGNLTS 675
            +    L     L + PD    +  +L T +I S           C  L+  P  + +  +
Sbjct: 932  IYKCGL-----LDFQPDHHEYSYRNLQTLSIKS----------SCDTLVKFP--LNHFAN 991

Query: 676  LHLLFIEGCSKLKSLP---EGMRKLKSLQCLSIRDCPQLSERCKEGGEDWPKISHIPNVA 702
            L  L ++ C+ L SL    E +R   +L+ L I DC  L        +  PK++ +P   
Sbjct: 992  LDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNL--------QLLPKLNALPQNL 1004

HSP 2 Score: 109.4 bits (272), Expect = 1.7e-22
Identity = 74/227 (32.60%), Postives = 111/227 (48.90%), Query Frame = 0

Query: 32  EDDLNKLKSNLSAIQAVLFDAEEHPTKRHSLKDCLEKLGEVLYDADDVLDEFSCEALHRE 91
           E+ L +L + L  I AVL DAEE       ++  + +L +V+Y A+D LD+ + EAL   
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  VMTGGKNAKQLNKI----------------FEPRIQH---------DSSHQIGTK----- 151
           +     ++ +L ++                 E R++             + +G K     
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155

Query: 152 -----WETHSSLCGEEVIGRDDDKMHLKSLLLDENVKDN-VSFIAIVGMGGIGKTTLAKF 211
                  T S +   EV GRDDDK  +   L+ EN KDN ++ +AIVG+GG+GKTTL++ 
Sbjct: 156 IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQL 215

Query: 212 LYNDSEVSDCFELKMWVWVSEQFDEKIIVKKIIESATDSQPNAQEMD 223
           LYND  V   F  K+W  VSE+FD   I KK+ ES T       ++D
Sbjct: 216 LYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLD 262

BLAST of Lag0002753 vs. ExPASy TrEMBL
Match: A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 1.8e-168
Identity = 417/1054 (39.56%), Postives = 516/1054 (48.96%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRH 60
            MAEG+L  +AGEIL KL S  S   GML G +DDL++LKS +S IQ+ L DA+  PT   
Sbjct: 255  MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNLPTGLD 314

Query: 61   SLKDCLEKLGEVLYDADDVLDEFSCEALHREVMTGGKNAKQLNKIFEPRIQHDSSHQIGT 120
            S+   LEKL  VL+DA+D++DEF  E  HREVMT  K+AK++   F    Q   ++++  
Sbjct: 315  SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 374

Query: 121  K------------------W------------------------ETHSSLCGEEVIGRDD 180
            +                  W                        ETHSSL  E VIGRDD
Sbjct: 375  RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRMRMSLSRETHSSLNKERVIGRDD 434

Query: 181  DKMHLKSLLL--DENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSE 240
            DK +LKSLLL  D ++KDNVS IAIVGMGGIGKTTLAK LYND +VS  FE+KMW+WVS+
Sbjct: 435  DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSKHFEIKMWIWVSK 494

Query: 241  QFDEKIIVKKIIESATDSQPNAQEMD---------------------------------- 300
            QF EKI+VKKI+ESAT   P  QE+D                                  
Sbjct: 495  QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 554

Query: 301  -----------------------------------------PWILY-NVSFK-------- 360
                                                      W L+  V+F+        
Sbjct: 555  DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDRSWSLFREVAFEGNLPNPIN 614

Query: 361  ------------------------------------------------------------ 420
                                                                        
Sbjct: 615  PKLELMGKEISSRCGGVPLAIRHMGRFLCSKRCEEEWMSSLSDKLLEVTQQNNDVASILE 674

Query: 421  ------------------------------------------------------------ 480
                                                                        
Sbjct: 675  LSYNDLPLHLKQCFAYSSLFPKVHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFDE 734

Query: 481  ----------SDEYNFDDRVHMHDVMRELSKKVAGMKLYVHGNPNNDYDVSEQTRHISFD 540
                      SDE NFDD V+MHDVM EL++KVA  KLYV G+ NN+Y VSEQT H SFD
Sbjct: 735  LCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLYVCGDINNNYVVSEQTLHFSFD 794

Query: 541  -KIESWQAVLSKLRKAKGLRTFLLLHSYYEEWNE-------------------------- 600
             KI+SWQ VLSKL KAKGLRTF+ LHS YEE NE                          
Sbjct: 795  YKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKNEVNEANLDELFSSFPRLRVLLLGGSNI 854

Query: 601  ----------------------------------------LTTCLSLKELPRNTSNLINL 660
                                                    LT C +LKE+PR+ + L+NL
Sbjct: 855  HVLPNSIKKLKLLRYLDLSENNMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKLVNL 914

Query: 661  RHLDF-SSKKIRHM-PKGMEKLTCLQTISLFELDCKRFSKLSELNKFNNLSAKSLRIIGL 701
            RHLD     +I HM P+GMEKL+CLQTISLF  DC + +KL ELN+ NNL+ + L+I GL
Sbjct: 915  RHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKTNKLRELNELNNLTGE-LKITGL 974

BLAST of Lag0002753 vs. ExPASy TrEMBL
Match: A0A5A7UWP6 (Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00750 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 2.3e-168
Identity = 417/1055 (39.53%), Postives = 516/1055 (48.91%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRH 60
            MAEG+L  +AGEIL KL S  S   GML G +DDL++LKS +S IQ+ L DA+  PT   
Sbjct: 1    MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNWPTGLD 60

Query: 61   SLKDCLEKLGEVLYDADDVLDEFSCEALHREVMTGGKNAKQLNKIFEPRIQHDSSHQIGT 120
            S+   LEKL  VL+DA+D++DEF  E  HREVMT  K+AK++   F    Q   ++++  
Sbjct: 61   SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 120

Query: 121  K------------------W------------------------ETHSSLCGEEVIGRDD 180
            +                  W                        ETHSSL  E VIGRDD
Sbjct: 121  RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRMRMSSSRETHSSLNKERVIGRDD 180

Query: 181  DKMHLKSLLL--DENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSE 240
            DK +LKSLLL  D ++KDNVS IAIVGMGGIGKTTLAK LYND +VS+ FE+KMW+WVS+
Sbjct: 181  DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSEHFEIKMWIWVSK 240

Query: 241  QFDEKIIVKKIIESATDSQPNAQEMD---------------------------------- 300
            QF EKI+VKKI+ESAT   P  QE+D                                  
Sbjct: 241  QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 300

Query: 301  -----------------------------------------PWILY-------------- 360
                                                      W L+              
Sbjct: 301  DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDSSWSLFREVAFEENLPKPIN 360

Query: 361  ---------------------------------------NVSFK---------------- 420
                                                   ++S+K                
Sbjct: 361  PKLELMGKEISSICGGVPLAIRHMGRFLCSKRCEEEWMSSLSWKKHLEVAQQNNDVASIL 420

Query: 421  ------------------------------------------------------------ 480
                                                                        
Sbjct: 421  ELSYNDLPLHLKQCFAYSSLFPKGHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFD 480

Query: 481  -----------SDEYNFDDRVHMHDVMRELSKKVAGMKLYVHGNPNNDYDVSEQTRHISF 540
                       SDE NFDD V+MHDVM EL++KVA  KL+V G+ NN+Y VSEQT H S 
Sbjct: 481  ELCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLFVCGDINNNYVVSEQTLHFSL 540

Query: 541  D-KIESWQAVLSKLRKAKGLRTFLLLHSYYEEWNE------------------------- 600
            D KI+SWQ VLSKL KAKGLRTF+ LHS YEE NE                         
Sbjct: 541  DYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKNEVNEANLDELFSSFPRLRVLLLGGSN 600

Query: 601  -----------------------------------------LTTCLSLKELPRNTSNLIN 660
                                                     LT C +LKE+PR+ + L+N
Sbjct: 601  IHVLPNSIKKLKLLRYLDLSENNMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKLVN 660

Query: 661  LRHLDF-SSKKIRHM-PKGMEKLTCLQTISLFELDCKRFSKLSELNKFNNLSAKSLRIIG 701
            LRHLD     +I HM P+GMEKL+CLQTISLF  DC + +KL ELN+ NNL+ + L+I G
Sbjct: 661  LRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKTNKLRELNELNNLTGE-LKITG 720

BLAST of Lag0002753 vs. ExPASy TrEMBL
Match: A0A6J1D168 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111016078 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 6.0e-156
Identity = 413/1058 (39.04%), Postives = 505/1058 (47.73%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEH-PTKR 60
            MAEGVLF +AGEILMKL S AS+RLGM+ G E+DL KL S +S I+ VL DAEEH  TK 
Sbjct: 1    MAEGVLFHVAGEILMKLGSIASQRLGMVRGVENDLTKLTSTVSVIKNVLLDAEEHCQTKT 60

Query: 61   -HSLKDCLEKLGEVLYDADDVLDEFSCEALHREVMTGGKNAKQLNKIF------------ 120
             H+L+D L+KL E LYDA+DVLDE S E LHREV+T GKNAKQ+   F            
Sbjct: 61   DHTLQDWLQKLEEALYDAEDVLDELSSETLHREVVTKGKNAKQVRIFFSGSNQIAFNYRV 120

Query: 121  ---------------------------EPRIQHDSSHQI--GTKWETHSSLCGEEVIGRD 180
                                       + R  + S  QI  G K ET S    EEVIGRD
Sbjct: 121  ARNIKKIWERLDAIDAERKQFNLTENCQQRTLYGSFDQIMMGRK-ETCSFPNVEEVIGRD 180

Query: 181  DDKMHLKSLLLD--ENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVS 240
             DK  LK LLLD   NV+ NVSFI I GMGGIGKTTLAK LY+D EVS+CF+  +W+W+S
Sbjct: 181  YDKKKLKDLLLDINMNVRHNVSFIVIAGMGGIGKTTLAKSLYHDREVSECFDSTIWIWIS 240

Query: 241  EQFDEKIIVKKIIESATDSQPNA------------------------------------- 300
            +QF+ +++V+KIIESAT  +PN                                      
Sbjct: 241  DQFEVRVVVEKIIESATKRKPNVEGMEALNSELQEVIGGKKYLLVMDDVWKETEEKWHEL 300

Query: 301  --------------------------------------QEMDPWILYN------------ 360
                                                  QE + W L+             
Sbjct: 301  KPLLMGGASGSKILITKRDSKVATEIESMTSLFTLKGLQESNSWSLFRKVAFKEAKDPAN 360

Query: 361  -------------------------------------VSFK------------------- 420
                                                 +SFK                   
Sbjct: 361  SNLIKLGKEILAKCGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLKVIQQTDKNDGLTP 420

Query: 421  ------------------------------------------------------------ 480
                                                                        
Sbjct: 421  ILNLSYNHLPPNLKQCFAYLSLFPKGTRLEIKDLIRQWMAHGFIESSNGTANSMEEVGKS 480

Query: 481  --------------SDEYNFDDRVHMHDVMRELSKKVAGMKLYVHGNPNNDYDVSEQTRH 540
                          SDE NFDD V MHDVMRE++  VAG K YVHGN N D+ VSE+TRH
Sbjct: 481  YFKELCWRFFFENSSDECNFDDDVDMHDVMREVAMNVAGEK-YVHGNLNCDFVVSEKTRH 540

Query: 541  ISFD-KIESWQAVLSKLRKAKGLRTFLLLHSYYEEWN----------------------- 600
            I FD KI+ WQ VL KL+KAKGLRTFL L SY+   N                       
Sbjct: 541  ILFDSKIKLWQDVLLKLQKAKGLRTFLFL-SYFPFKNRLEDNIEAVLEELCSSFPRLRVL 600

Query: 601  ----------------------------------------------ELTTCLSLKELPRN 660
                                                          +L  C  L ELPR+
Sbjct: 601  GLNHSKIHVVPNSIKNLRHLRYLNLSRNYMESLPTSITKLQNLQSLDLKDCYKLNELPRD 660

Query: 661  TSNLINLRHL---DFSSKKIRHMPKGMEKLTCLQTISLFELDCKRFSKLSELNKFNNLSA 695
            TSNL+NLRHL   D     + HM +GM KL+CL+T+  F LDCKR +  SELN  N  + 
Sbjct: 661  TSNLVNLRHLVLPDHLDTPL-HMLEGMRKLSCLRTLGYFVLDCKRSNMQSELNVPNYFNG 720

BLAST of Lag0002753 vs. ExPASy TrEMBL
Match: A0A5D3CJL8 (Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00520 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 9.0e-152
Identity = 389/1022 (38.06%), Postives = 499/1022 (48.83%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRH 60
            MAEG+L  +AGEIL KL S  S   GML G +DDL++LKS +S IQ+ L DA+  PT   
Sbjct: 1    MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNWPTGLD 60

Query: 61   SLKDCLEKLGEVLYDADDVLDEFSCEALHREVMTGGKNAKQLNKIFEPRIQHDSSHQIGT 120
            S+   LEKL  VL+DA+D++DEF  E  HREVMT  K+AK++   F    Q   ++++  
Sbjct: 61   SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 120

Query: 121  K------------------W------------------------ETHSSLCGEEVIGRDD 180
            +                  W                        ETHSSL  E VIGRDD
Sbjct: 121  RVKRIRERLDVIDKEKSYLWSHGFVQGRQQDVPSPIRMRMSSSRETHSSLNKERVIGRDD 180

Query: 181  DKMHLKSLLL--DENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSE 240
            DK +LKSLLL  D ++KDNVS IAIVGMGGIGKTTLAK LYND +VS  FE+KMW+WVS+
Sbjct: 181  DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSKHFEIKMWIWVSK 240

Query: 241  QFDEKIIVKKIIESATDSQPNAQEMD---------------------------------- 300
            QF EKI+VKKI+ESAT   P  QE+D                                  
Sbjct: 241  QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 300

Query: 301  -----------------------------------------PWILY-NVSFKSD------ 360
                                                      W L+  V+F+ +      
Sbjct: 301  DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDSSWSLFREVAFEENLPKPIN 360

Query: 361  ------------------------------------------------------------ 420
                                                                        
Sbjct: 361  PKLELMGKEISSICGGVPLAIRHMGRFLCSKRCEEEWMSSLSWKKHLEVAQQNNDVASIL 420

Query: 421  EYNFDD-------------------RVHMHDVMR--------ELSKKVAGMK-------- 480
            E +++D                   ++ +++++R        E SK    M+        
Sbjct: 421  ELSYNDLPLHLKQCFAYSSLFPKGHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFD 480

Query: 481  ------LYVHGNPNNDYDVSEQTRHISFDKIESWQAVLSKLRKAKGLRTFLLLHSYYEEW 540
                   Y + +  N++D       + ++     + VLSKL KAKGLRTF+ LHS YEE 
Sbjct: 481  ELCWRFFYENSSDENNFDDYVYMHDVMYELAR--KDVLSKLCKAKGLRTFMFLHSPYEEK 540

Query: 541  NE---------------------------------------------------------- 600
            NE                                                          
Sbjct: 541  NEVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSENNMKSLPNSITE 600

Query: 601  --------LTTCLSLKELPRNTSNLINLRHLDF-SSKKIRHM-PKGMEKLTCLQTISLFE 660
                    LT C +LKE+PR+ + L+NLRHLD     +I HM P+GMEKL+CLQTISLF 
Sbjct: 601  LQDLQTLKLTGCYALKEMPRDINKLVNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFV 660

Query: 661  LDCKRFSKLSELNKFNNLSAKSLRIIGLEQLRSTSFEVSSVNLKEKTNFRRLILEWEL-- 701
             DC + +KL ELN+ NNL+ + L+I GL+QLRST +EVS VNLK K   R L LEW++  
Sbjct: 661  FDCTKTNKLRELNELNNLTGE-LKITGLQQLRSTPYEVSLVNLKGKKCLRSLKLEWKMGL 720

BLAST of Lag0002753 vs. ExPASy TrEMBL
Match: A0A6J1GPK9 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111456321 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 9.6e-146
Identity = 394/1103 (35.72%), Postives = 491/1103 (44.51%), Query Frame = 0

Query: 1    MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRH 60
            MAEGVLF +A +I+ KL+S AS++LGML G +D+L +LKS++SAI+AV+ DAEE  TK H
Sbjct: 1    MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60

Query: 61   SLKDCLEKLGEVLYDADDVLDEFSCEALHREVMTGGKNAKQ------------------- 120
             LKD LEKL E LYDA+DVLDE S EA+ R     G+ AKQ                   
Sbjct: 61   FLKDWLEKLKEALYDAEDVLDEISGEAVRR-----GRKAKQVRIFFSRYNPIAFDYKMTR 120

Query: 121  --------------------LNKIFEPRIQHDSSHQIGTKWETHSSLCGEEVIGRDDDKM 180
                                L +I   + QH S  QI    ETHS    EEVIGRDD K 
Sbjct: 121  QIKNVRERLGVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKR 180

Query: 181  HLKSLLLDENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSEQFDEK 240
             +K LL D NVKDN+SFIAIVGM GIGKTTLA+ L+NDS+VS CF+LK+WVWVS+ F+ K
Sbjct: 181  VIKDLLQDMNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEVK 240

Query: 241  IIVKKIIESATDSQPNA------------------------------------------- 300
            I+V+KIIESAT+S+PN                                            
Sbjct: 241  IVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLMG 300

Query: 301  --------------------------------QEMDPWILYN------------------ 360
                                             E D W L+                   
Sbjct: 301  GARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQL 360

Query: 361  -------------------------------VSFK------------------------- 420
                                           +SFK                         
Sbjct: 361  GKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLSYNHL 420

Query: 421  ------------------------------------------------------------ 480
                                                                        
Sbjct: 421  PSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELCWRFF 480

Query: 481  ----SDEYNFDDRVHMHDVMRELSKKVAGMKLYVHGNPNNDYDVSEQTRHISFDK-IESW 540
                +DE+NF+D V++HDVM +L++ VAG + YV GN N+D+ V E TRHI FD+ I  W
Sbjct: 481  YENFNDEHNFEDDVYIHDVMHDLARMVAGNE-YVGGNQNHDHVVGESTRHILFDREIGIW 540

Query: 541  QAVLSKLRKAKGLRTFLLLHSYYEEWNE-------------------------------- 600
            + V SKLRKAKGLRTF+L +   +  N+                                
Sbjct: 541  EHVPSKLRKAKGLRTFILSNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRVPDS 600

Query: 601  ----------------------------------LTTCLSLKELPRNTSNLINLRHLDFS 660
                                              L  C +LK+LPR T NL+NLRHL+ S
Sbjct: 601  IKRLTHLRYLDLSGNYMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHLNIS 660

Query: 661  -SKKIRHMPKGMEKLTCLQTISLFELDC---KRFSKLSELNKFNNLSAKSLRIIGLEQLR 696
             S  + HMP+GM KLTCLQT+S F LD    +  SKL ELN  N+L   SL IIGLE L+
Sbjct: 661  DSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRG-SLEIIGLENLK 720

BLAST of Lag0002753 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-24
Identity = 108/424 (25.47%), Postives = 179/424 (42.22%), Query Frame = 0

Query: 316  LTTCLSLKELPRNTSNLINLRHLDFSSKKIRHMPKGMEKLTCLQTISLFELDCKRFSKLS 375
            L+ C SLKELP + SNLINLR+LD    K+R MP+   +L  LQT++ F +     S++S
Sbjct: 632  LSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRIS 691

Query: 376  ELNKFNNLSAKSLRIIGLEQLRSTSFEVSSVNLKEKTNFRRLILEWELSADECE------ 435
            EL   ++L  K L+I+ L+++   + + +  NL  K + R +   W   +   E      
Sbjct: 692  ELGGLHDLHGK-LKIVELQRVVDVA-DAAEANLNSKKHLREIDFVWRTGSSSSENNTNPH 751

Query: 436  --------------------LVKLRYSGVGVPNWVSF-SLLKLTKITIANCHRLQHLPQF 495
                                L   RY G   P+W+S  S  ++  I +  C     LP  
Sbjct: 752  RTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSL 811

Query: 496  SHLEALEFLYLKDLRCLKFIDTNDPYSSSVF-------FPSLKKLYLEDMPNLKGWWELG 555
              L  L+ L++  +  L+ I     +S           F SL+ L  +++P+ + W ++ 
Sbjct: 812  GQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVR 871

Query: 556  ESRAGETENKCSPFIPELECLHIRHCPKLT-SMPPFIPTISVILCDVGVQLLSTIGPLSH 615
             +R            P L+ L I  CP+LT ++P F+P++  +                H
Sbjct: 872  VTR--------GDLFPSLKKLFILRCPELTGTLPTFLPSLISL----------------H 931

Query: 616  VEHLKLAEMKDLKYLPDEFHQNLLSLGTSTISSPIRPGVLQIEECPNLMSLPEWIGNLTS 675
            +    L     L + PD    +  +L T +I S           C  L+  P  + +  +
Sbjct: 932  IYKCGL-----LDFQPDHHEYSYRNLQTLSIKS----------SCDTLVKFP--LNHFAN 991

Query: 676  LHLLFIEGCSKLKSLP---EGMRKLKSLQCLSIRDCPQLSERCKEGGEDWPKISHIPNVA 702
            L  L ++ C+ L SL    E +R   +L+ L I DC  L        +  PK++ +P   
Sbjct: 992  LDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNL--------QLLPKLNALPQNL 1004

HSP 2 Score: 109.4 bits (272), Expect = 1.2e-23
Identity = 74/227 (32.60%), Postives = 111/227 (48.90%), Query Frame = 0

Query: 32  EDDLNKLKSNLSAIQAVLFDAEEHPTKRHSLKDCLEKLGEVLYDADDVLDEFSCEALHRE 91
           E+ L +L + L  I AVL DAEE       ++  + +L +V+Y A+D LD+ + EAL   
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  VMTGGKNAKQLNKI----------------FEPRIQH---------DSSHQIGTK----- 151
           +     ++ +L ++                 E R++             + +G K     
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155

Query: 152 -----WETHSSLCGEEVIGRDDDKMHLKSLLLDENVKDN-VSFIAIVGMGGIGKTTLAKF 211
                  T S +   EV GRDDDK  +   L+ EN KDN ++ +AIVG+GG+GKTTL++ 
Sbjct: 156 IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQL 215

Query: 212 LYNDSEVSDCFELKMWVWVSEQFDEKIIVKKIIESATDSQPNAQEMD 223
           LYND  V   F  K+W  VSE+FD   I KK+ ES T       ++D
Sbjct: 216 LYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLD 262

BLAST of Lag0002753 vs. TAIR 10
Match: AT3G46730.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 90.5 bits (223), Expect = 5.8e-18
Identity = 67/235 (28.51%), Postives = 105/235 (44.68%), Query Frame = 0

Query: 9   IAGEILMKLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRHSLKDCLEK 68
           + G +L K+  +    +  L G +DDL +LK+ L+ I   L D E    +    K+  + 
Sbjct: 5   VTGFVLNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEAREREDEVSKEWTKL 64

Query: 69  LGEVLYDADDVLDEFSCEALHREVMTG--------GKNAKQLNKIFEPRIQHDSSHQIGT 128
           + ++ YD +DVLD +  +   R +  G        GK     N + + R        I  
Sbjct: 65  VLDIAYDIEDVLDTYFLKLEERSLRRGLLRLTNKIGKKRDAYNIVEDIRTLKRRILDITR 124

Query: 129 KWET-----HSSLCGEE--------------------VIGRDDDKMHLKSLLLDENVKDN 188
           K ET      +   GE                     V+G +DD   L   LL +N KD 
Sbjct: 125 KRETFGIGSFNEPRGENITNVRVRQLRRAPPVDQEELVVGLEDDVKILLVKLLSDNEKDK 184

Query: 189 VSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSEQFDEKIIVKKIIES 211
              I+I GMGG+GKT LA+ LYN  +V   F+ + W +VS+++  + I+ +II S
Sbjct: 185 SYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILIRIIRS 239

BLAST of Lag0002753 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 90.1 bits (222), Expect = 7.5e-18
Identity = 74/260 (28.46%), Postives = 121/260 (46.54%), Query Frame = 0

Query: 1   MAEGVLFTIAGEILMKLTSHASRRLGMLCGFEDD---LNKLKSNLSAIQAVLFDAEEHPT 60
           MA   L + A  ++ ++  + S+ L  LC  +     L +LK  L     VL DA++   
Sbjct: 1   MANSYLSSCANVMVERI--NTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAE 60

Query: 61  KRHSLKDCLEKLGEVLYDADDVLDEFSCEALHREVMT-----GG------KNAKQLNKIF 120
               +K  L  + +  + A+D+LDE   EAL R V+      GG         + + K  
Sbjct: 61  HVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQKKI 120

Query: 121 EPR-------IQHDSSH--QIGTK---------WETHS-----SLCGEEVIGRDDDKMHL 180
           EP+       ++H   H   IG K         W   S      L    ++GR +DK+ L
Sbjct: 121 EPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQASRSRPDDLPQGRLVGRVEDKLAL 180

Query: 181 KSLLL--DENVKDNVSFIAIVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSEQFDEK 222
            +LLL  DE      + I++VGM G+GKTTL + ++ND  V++ FE+KMW+     F+  
Sbjct: 181 VNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVF 240

BLAST of Lag0002753 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 89.7 bits (221), Expect = 9.8e-18
Identity = 107/384 (27.86%), Postives = 167/384 (43.49%), Query Frame = 0

Query: 316  LTTCLSLKELPRNTSNLINLRHLDFSS-KKIRHMPKGMEKLTCLQTISLFELDCKRFSKL 375
            L+ C SL ELP +  NLINL+ LD S    +  +P  +  L  LQ + L E      S L
Sbjct: 867  LSGCSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIGNLINLQELYLSEC-----SSL 926

Query: 376  SEL-NKFNNLSAKSLRIIGLEQLRSTSFEVSSVNLKEKTNFRRLILEWELSADECELVKL 435
             EL +   NL   +L+ + L +  S     SS+          LI   EL   EC  +  
Sbjct: 927  VELPSSIGNLI--NLKTLNLSECSSLVELPSSIG--------NLINLQELYLSECSSLVE 986

Query: 436  RYSGVGVPNWVSFSLLKLTKITIANCHRLQHLP-QFSHLEALEFLYLKDLRCLKFIDTND 495
              S +G       +L+ L K+ ++ C  L  LP    +L  L+ L L +  C   ++   
Sbjct: 987  LPSSIG-------NLINLKKLDLSGCSSLVELPLSIGNLINLKTLNLSE--CSSLVELPS 1046

Query: 496  PYSSSVFFPSLKKLYLEDMPNLKGWWELGESRAGETENK------CSPFIP--------- 555
               + +   +L++LYL +  +L    EL  S       K      CS  +          
Sbjct: 1047 SIGNLI---NLQELYLSECSSLV---ELPSSIGNLINLKKLDLSGCSSLVELPLSIGNLI 1106

Query: 556  ELECLHIRHCPKLTSMPPFIPTISVILCDVG-----VQLLSTIGPLSHVEHLKLAEMKDL 615
             L+ L++  C  L  +P  I  +++   D+      V+L S+IG L +++ L L+    L
Sbjct: 1107 NLKTLNLSGCSSLVELPSSIGNLNLKKLDLSGCSSLVELPSSIGNLINLKKLDLSGCSSL 1166

Query: 616  KYLPDEFHQNLLSLGTSTISSPIRPGVLQIEECPNLMSLPEWIGNLTSLHLLFIEGCSKL 675
              LP       LS+G     + I    L + EC +L+ LP  IGNL +L  L++  CS L
Sbjct: 1167 VELP-------LSIG-----NLINLQELYLSECSSLVELPSSIGNLINLQELYLSECSSL 1208

Query: 676  KSLPEGMRKLKSLQCLSIRDCPQL 677
              LP  +  L +L+ L +  C +L
Sbjct: 1227 VELPSSIGNLINLKKLDLNKCTKL 1208

BLAST of Lag0002753 vs. TAIR 10
Match: AT1G58400.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 85.1 bits (209), Expect = 2.4e-16
Identity = 58/235 (24.68%), Postives = 115/235 (48.94%), Query Frame = 0

Query: 16  KLTSHASRRLGMLCGFEDDLNKLKSNLSAIQAVLFDAEEHPTKRHSLKDCLEKLGEVLYD 75
           KL    ++      G ED + +LKSNL+ +++ L DAE        ++ C+E++ E++YD
Sbjct: 12  KLWDRLTQEYEQFQGVEDRIAELKSNLNLLKSFLKDAEAKKNTSQMVRHCVEEIKEIVYD 71

Query: 76  ADDVLDEF-------------------SCEALHREVMTG--GKNAKQLNKIFEPR----I 135
            +++++ F                   +C  +HR       G  +K+++K+ +      +
Sbjct: 72  TENMIETFILKEAARKRSGIIRRITKLTCIKVHRWEFASDIGGISKRISKVIQDMHSFGV 131

Query: 136 QH------DSSHQIGTK----WETHSSLCGEEVIGRDDDKMHLKSLLLDENVKDNVSFIA 195
           Q        SSH +  +     +T S     + +G + +   L   L++E   D++  ++
Sbjct: 132 QQMISDGSQSSHLLQEREREMRQTFSRGYESDFVGLEVNVKKLVGYLVEE---DDIQIVS 191

Query: 196 IVGMGGIGKTTLAKFLYNDSEVSDCFELKMWVWVSEQFDEKIIVKKIIESATDSQ 216
           + GMGG+GKTTLA+ ++N  +V   F+   WV VS++F  K + + I+++ T  +
Sbjct: 192 VTGMGGLGKTTLARQVFNHEDVKHQFDRLAWVCVSQEFTRKNVWQMILQNLTSRE 243