Lag0002751 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002751
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptiondisease resistance protein RGA2
Locationchr4: 45320765 .. 45321976 (+)
RNA-Seq ExpressionLag0002751
SyntenyLag0002751
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGAAGGATTTCTCTCCACTCTTGCTGCGGAGATCTTGATGAAGCTAACCTCTCAAGCTTCACAACGGCTTGAAATGCTAAGCGGACTCAAGGATGATCTTAACAAACTCGAGAGCAATGTTTCAGCCATTCAGGCTGTACTTCTTGATGCCGAGGAGCAACCAACTAAAAGTCACTCCCTAAAGGATTGGCTCGAAAAGTTGGGAGATGCTCTTTATGATGCAGATGATGTGCTTGATGAATTATCTGGTGAGGCTCTCCATCGTGAAGTGATGACAGGAGGAAAAAATGCAAAACAGGTAAGGATCTTTTTCTCCAGATCTAACCAACTTGCATTTAATTATAGGATGGCACGTGAAGTAAAGAAAATTAGGGAGAGGTTAGATGTTATTTCTTCTGAAAAGGATAATTTTCATTTGACTAATGAAACATCTAGATCAAGGATACATCATGATTTATCTCATCAAATTGGCACGAAACGGGATACTCACTCTTCTTTAAAAGGAAAGGAAGTGATCGGAAGGGATGATGATAAGATGCACCTAAAAAGCCTTTTACTAGATATGAATGTCGAAGGCAACGTTTCATTCATTGCAATAGTTGGAATGGGGGGGATAGGCAAGACAACTCTAGCTAAATTTCTCTACAATGACAATGAAGTATCAAACTTTTTCGAGCTAAAAATATGGATTTGGGTTTCTGAACAATTTGATGCAAAAATAGTAGTGGAAAAAATTATAGAATCGGCAACTGGTAGCAAACCTAATGTGCAAGAGATGGATCCTTTACAACGTGACCTTCAAGAAGTGATCGGAGAAAAGAAGTATTTGTTGGTTATGGATGATATATGGAATGAAAGTGGAGAAAAATGGGAGGACTTGAAACCGTTGTTAATGGGTGGAGCGAGAGGGAGTAAGATAATGATCACAAAGCGCGATAATAAAGTAGCTGCAGAAATTGGAAGAATGACTACGTTGTTCACTTTAAAAGGCTTACATGAAAACGATTCTTGGCCATTGTTTAGAGAAGTGGCATTCAAAGATGGCAAAGAGCCAGCAAATCCAAACCTGATACAATTGGGAAAAGAAATTTTAGCAAAATGTGGAGGTGTTCCTCTTGTAATAAGACACATAGGAGGCTTGCTATACTCTAAAACTTCAGAAGAAGAGTGGACATCCTTCAAGAATAACGAACTTTTGAAAGTATGA

mRNA sequence

ATGGCCGAAGGATTTCTCTCCACTCTTGCTGCGGAGATCTTGATGAAGCTAACCTCTCAAGCTTCACAACGGCTTGAAATGCTAAGCGGACTCAAGGATGATCTTAACAAACTCGAGAGCAATGTTTCAGCCATTCAGGCTGTACTTCTTGATGCCGAGGAGCAACCAACTAAAAGTCACTCCCTAAAGGATTGGCTCGAAAAGTTGGGAGATGCTCTTTATGATGCAGATGATGTGCTTGATGAATTATCTGGTGAGGCTCTCCATCGTGAAGTGATGACAGGAGGAAAAAATGCAAAACAGGTAAGGATCTTTTTCTCCAGATCTAACCAACTTGCATTTAATTATAGGATGGCACGTGAAGTAAAGAAAATTAGGGAGAGGTTAGATGTTATTTCTTCTGAAAAGGATAATTTTCATTTGACTAATGAAACATCTAGATCAAGGATACATCATGATTTATCTCATCAAATTGGCACGAAACGGGATACTCACTCTTCTTTAAAAGGAAAGGAAGTGATCGGAAGGGATGATGATAAGATGCACCTAAAAAGCCTTTTACTAGATATGAATGTCGAAGGCAACGTTTCATTCATTGCAATAGTTGGAATGGGGGGGATAGGCAAGACAACTCTAGCTAAATTTCTCTACAATGACAATGAAGTATCAAACTTTTTCGAGCTAAAAATATGGATTTGGGTTTCTGAACAATTTGATGCAAAAATAGTAGTGGAAAAAATTATAGAATCGGCAACTGGTAGCAAACCTAATGTGCAAGAGATGGATCCTTTACAACGTGACCTTCAAGAAGTGATCGGAGAAAAGAAGTATTTGTTGGTTATGGATGATATATGGAATGAAAGTGGAGAAAAATGGGAGGACTTGAAACCGTTGTTAATGGGTGGAGCGAGAGGGAGTAAGATAATGATCACAAAGCGCGATAATAAAGTAGCTGCAGAAATTGGAAGAATGACTACGTTGTTCACTTTAAAAGGCTTACATGAAAACGATTCTTGGCCATTGTTTAGAGAAGTGGCATTCAAAGATGGCAAAGAGCCAGCAAATCCAAACCTGATACAATTGGGAAAAGAAATTTTAGCAAAATGTGGAGGTGTTCCTCTTGTAATAAGACACATAGGAGGCTTGCTATACTCTAAAACTTCAGAAGAAGAGTGGACATCCTTCAAGAATAACGAACTTTTGAAAGTATGA

Coding sequence (CDS)

ATGGCCGAAGGATTTCTCTCCACTCTTGCTGCGGAGATCTTGATGAAGCTAACCTCTCAAGCTTCACAACGGCTTGAAATGCTAAGCGGACTCAAGGATGATCTTAACAAACTCGAGAGCAATGTTTCAGCCATTCAGGCTGTACTTCTTGATGCCGAGGAGCAACCAACTAAAAGTCACTCCCTAAAGGATTGGCTCGAAAAGTTGGGAGATGCTCTTTATGATGCAGATGATGTGCTTGATGAATTATCTGGTGAGGCTCTCCATCGTGAAGTGATGACAGGAGGAAAAAATGCAAAACAGGTAAGGATCTTTTTCTCCAGATCTAACCAACTTGCATTTAATTATAGGATGGCACGTGAAGTAAAGAAAATTAGGGAGAGGTTAGATGTTATTTCTTCTGAAAAGGATAATTTTCATTTGACTAATGAAACATCTAGATCAAGGATACATCATGATTTATCTCATCAAATTGGCACGAAACGGGATACTCACTCTTCTTTAAAAGGAAAGGAAGTGATCGGAAGGGATGATGATAAGATGCACCTAAAAAGCCTTTTACTAGATATGAATGTCGAAGGCAACGTTTCATTCATTGCAATAGTTGGAATGGGGGGGATAGGCAAGACAACTCTAGCTAAATTTCTCTACAATGACAATGAAGTATCAAACTTTTTCGAGCTAAAAATATGGATTTGGGTTTCTGAACAATTTGATGCAAAAATAGTAGTGGAAAAAATTATAGAATCGGCAACTGGTAGCAAACCTAATGTGCAAGAGATGGATCCTTTACAACGTGACCTTCAAGAAGTGATCGGAGAAAAGAAGTATTTGTTGGTTATGGATGATATATGGAATGAAAGTGGAGAAAAATGGGAGGACTTGAAACCGTTGTTAATGGGTGGAGCGAGAGGGAGTAAGATAATGATCACAAAGCGCGATAATAAAGTAGCTGCAGAAATTGGAAGAATGACTACGTTGTTCACTTTAAAAGGCTTACATGAAAACGATTCTTGGCCATTGTTTAGAGAAGTGGCATTCAAAGATGGCAAAGAGCCAGCAAATCCAAACCTGATACAATTGGGAAAAGAAATTTTAGCAAAATGTGGAGGTGTTCCTCTTGTAATAAGACACATAGGAGGCTTGCTATACTCTAAAACTTCAGAAGAAGAGTGGACATCCTTCAAGAATAACGAACTTTTGAAAGTATGA

Protein sequence

MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSHSLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAREVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDKMHLKSLLLDMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFDAKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKPLLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLKV
Homology
BLAST of Lag0002751 vs. NCBI nr
Match: XP_038897566.1 (putative disease resistance protein RGA4 [Benincasa hispida])

HSP 1 Score: 525.4 bits (1352), Expect = 4.3e-145
Identity = 278/405 (68.64%), Postives = 326/405 (80.49%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAE  L  +A EILMKL+SQA QRL ML GLKD+LNKL + VS I+ VLLDAE + TK H
Sbjct: 1   MAEAILFDIAGEILMKLSSQAFQRLGMLFGLKDNLNKLTATVSTIKDVLLDAEGRQTKGH 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAR 120
            L++WL+KL +ALYDA+DVLDELS EALHREVMT  KNAKQVRIFFS+SNQ+AFNY MAR
Sbjct: 61  LLENWLQKLEEALYDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQIAFNYSMAR 120

Query: 121 EVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDK 180
           ++KKI ERLD I +EK  FHL +E   S+  +    QI T R++ S   G+EVIGRDDDK
Sbjct: 121 QIKKIWERLDAIDAEKKQFHL-HENCESKARYGSFDQIMTGRESWSFSNGEEVIGRDDDK 180

Query: 181 MHLKSLLLD--MNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQF 240
             +K LLLD  MNV  NVS +AIVGMGGIGKTTLAK LYND ++S +FELKIWIWVS+QF
Sbjct: 181 KKIKDLLLDVNMNVTHNVSIVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWIWVSDQF 240

Query: 241 DAKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKPL 300
           + K VV+KIIESAT S P+V  M+ LQ +LQ+VIG KKYLLVMDD+WNES EKW  LKPL
Sbjct: 241 EIKAVVKKIIESATKSNPHVMGMEALQAELQKVIGGKKYLLVMDDVWNESEEKWHGLKPL 300

Query: 301 LMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNL 360
           LM GA+GSKI+ITKRD KVAAEI  MT+LF+L+GL E+ SW LF +VAFK+GKE  NPNL
Sbjct: 301 LMSGAKGSKILITKRDRKVAAEIESMTSLFSLEGLPESKSWSLFSKVAFKEGKELENPNL 360

Query: 361 IQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLKV 404
           IQ GKEIL KCGGVPLVIRHIG LLYS+T+EEEW SFK+NELL++
Sbjct: 361 IQTGKEILMKCGGVPLVIRHIGRLLYSRTTEEEWMSFKDNELLEI 404

BLAST of Lag0002751 vs. NCBI nr
Match: XP_022147051.1 (putative disease resistance protein RGA4 [Momordica charantia])

HSP 1 Score: 514.6 bits (1324), Expect = 7.6e-142
Identity = 273/407 (67.08%), Postives = 321/407 (78.87%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEE--QPTK 60
           MAEG L  +A EILMKL S ASQRL M+ G+++DL KL S VS I+ VLLDAEE  Q   
Sbjct: 1   MAEGVLFHVAGEILMKLGSIASQRLGMVRGVENDLTKLTSTVSVIKNVLLDAEEHCQTKT 60

Query: 61  SHSLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRM 120
            H+L+DWL+KL +ALYDA+DVLDELS E LHREV+T GKNAKQVRIFFS SNQ+AFNYR+
Sbjct: 61  DHTLQDWLQKLEEALYDAEDVLDELSSETLHREVVTKGKNAKQVRIFFSGSNQIAFNYRV 120

Query: 121 AREVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDD 180
           AR +KKI ERLD I +E+  F+LT    +  ++      +  +++T S    +EVIGRD 
Sbjct: 121 ARNIKKIWERLDAIDAERKQFNLTENCQQRTLYGSFDQIMMGRKETCSFPNVEEVIGRDY 180

Query: 181 DKMHLKSLLLD--MNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSE 240
           DK  LK LLLD  MNV  NVSFI I GMGGIGKTTLAK LY+D EVS  F+  IWIW+S+
Sbjct: 181 DKKKLKDLLLDINMNVRHNVSFIVIAGMGGIGKTTLAKSLYHDREVSECFDSTIWIWISD 240

Query: 241 QFDAKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLK 300
           QF+ ++VVEKIIESAT  KPNV+ M+ L  +LQEVIG KKYLLVMDD+W E+ EKW +LK
Sbjct: 241 QFEVRVVVEKIIESATKRKPNVEGMEALNSELQEVIGGKKYLLVMDDVWKETEEKWHELK 300

Query: 301 PLLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANP 360
           PLLMGGA GSKI+ITKRD+KVA EI  MT+LFTLKGL E++SW LFR+VAFK+ K+PAN 
Sbjct: 301 PLLMGGASGSKILITKRDSKVATEIESMTSLFTLKGLQESNSWSLFRKVAFKEAKDPANS 360

Query: 361 NLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLKV 404
           NLI+LGKEILAKCGGVPLVIRHIG LLYSKTSEEEW SFKNNELLKV
Sbjct: 361 NLIKLGKEILAKCGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLKV 407

BLAST of Lag0002751 vs. NCBI nr
Match: XP_022953917.1 (putative disease resistance protein RGA4 [Cucurbita moschata])

HSP 1 Score: 507.3 bits (1305), Expect = 1.2e-139
Identity = 265/403 (65.76%), Postives = 321/403 (79.65%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAEG L  LAA+I+ KL+S ASQ+L ML G+KD+L +L+S+VSAI+AV+LDAEE+ TKSH
Sbjct: 1   MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAR 120
            LKDWLEKL +ALYDA+DVLDE+SGEA+ R     G+ AKQVRIFFSR N +AF+Y+M R
Sbjct: 61  FLKDWLEKLKEALYDAEDVLDEISGEAVRR-----GRKAKQVRIFFSRYNPIAFDYKMTR 120

Query: 121 EVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDK 180
           ++K +RERL VI++E+ NFHLT E    +  H    QI    +THS    +EVIGRDD K
Sbjct: 121 QIKNVRERLGVIAAERKNFHLT-EIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHK 180

Query: 181 MHLKSLLLDMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFDA 240
             +K LL DMNV+ N+SFIAIVGM GIGKTTLA+ L+ND++VS  F+LKIW+WVS+ F+ 
Sbjct: 181 RVIKDLLQDMNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEV 240

Query: 241 KIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKPLLM 300
           KIVVEKIIESAT S+PNV  M+ LQ  LQ+++  KKYLLVMDDIWNES +KW +LK LLM
Sbjct: 241 KIVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLM 300

Query: 301 GGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQ 360
           GGARGSKIMITKRD+ +A EI  MT+L TLKGL E+DSW LF++VAF DG EP NP LIQ
Sbjct: 301 GGARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQ 360

Query: 361 LGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLKV 404
           LGKEIL KCGGVPLVIRHIG LLY + SEEEW SFKNN+LL+V
Sbjct: 361 LGKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEV 397

BLAST of Lag0002751 vs. NCBI nr
Match: XP_031745107.1 (putative disease resistance protein RGA4 [Cucumis sativus])

HSP 1 Score: 506.9 bits (1304), Expect = 1.6e-139
Identity = 270/406 (66.50%), Postives = 320/406 (78.82%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAE  L  +A EILMKL+SQA QRL ML GLK DLNKL + VS I+ VLLDAE + TKSH
Sbjct: 1   MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMT-GGKNAKQVRIFFSRSNQLAFNYRMA 120
            L++WL KL +ALYDA+DVLDELS EAL RE+MT   KNAKQVRIFFS+SNQ+AFNYRMA
Sbjct: 61  LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120

Query: 121 REVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDD 180
           R++K I ERLD I +EK  FHL  E   SR  +    +I   R+T SS   +EVIGRDDD
Sbjct: 121 RQIKNIWERLDAIDAEKTQFHL-RENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDD 180

Query: 181 KMHLKSLLLD--MNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQ 240
              +K  LLD  MNV  NVSFIAI GMGGIGKTTLAK LYND EVS FF+LKIW+WVS+Q
Sbjct: 181 IKEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQ 240

Query: 241 FDAKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKP 300
           F+ ++V EK+IESAT + P+V+ M+ LQ  LQ+VIGE+KYLLVMDD+WNES EKW  LK 
Sbjct: 241 FEVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKS 300

Query: 301 LLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPN 360
           LLMGGARGSK++ITKRD KVA EI  MT+LFTL+GL E++SW LF +VAFK+GKE  +P+
Sbjct: 301 LLMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPS 360

Query: 361 LIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLKV 404
            I LGKEIL +CGGVPLVIRH+G +LYSKTS+EEW SFK+NELL+V
Sbjct: 361 TIHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEV 405

BLAST of Lag0002751 vs. NCBI nr
Match: KAE8646395.1 (hypothetical protein Csa_016171 [Cucumis sativus])

HSP 1 Score: 506.9 bits (1304), Expect = 1.6e-139
Identity = 270/406 (66.50%), Postives = 320/406 (78.82%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAE  L  +A EILMKL+SQA QRL ML GLK DLNKL + VS I+ VLLDAE + TKSH
Sbjct: 1   MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMT-GGKNAKQVRIFFSRSNQLAFNYRMA 120
            L++WL KL +ALYDA+DVLDELS EAL RE+MT   KNAKQVRIFFS+SNQ+AFNYRMA
Sbjct: 61  LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120

Query: 121 REVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDD 180
           R++K I ERLD I +EK  FHL  E   SR  +    +I   R+T SS   +EVIGRDDD
Sbjct: 121 RQIKNIWERLDAIDAEKTQFHL-RENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDD 180

Query: 181 KMHLKSLLLD--MNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQ 240
              +K  LLD  MNV  NVSFIAI GMGGIGKTTLAK LYND EVS FF+LKIW+WVS+Q
Sbjct: 181 IKEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQ 240

Query: 241 FDAKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKP 300
           F+ ++V EK+IESAT + P+V+ M+ LQ  LQ+VIGE+KYLLVMDD+WNES EKW  LK 
Sbjct: 241 FEVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKS 300

Query: 301 LLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPN 360
           LLMGGARGSK++ITKRD KVA EI  MT+LFTL+GL E++SW LF +VAFK+GKE  +P+
Sbjct: 301 LLMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPS 360

Query: 361 LIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLKV 404
            I LGKEIL +CGGVPLVIRH+G +LYSKTS+EEW SFK+NELL+V
Sbjct: 361 TIHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEV 405

BLAST of Lag0002751 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.6e-70
Identity = 156/401 (38.90%), Postives = 233/401 (58.10%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAE FL  L    L  LTS    +L ++ G + +  KL S  S IQAVL DA+E+  K  
Sbjct: 1   MAEAFLQVL----LENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAR 120
           ++++WL+KL  A Y+ DD+L E   EA+  E         Q R+ F     + F +++ R
Sbjct: 61  AIENWLQKLNSAAYEVDDILGECKNEAIRFE---------QSRLGFYHPGIINFRHKIGR 120

Query: 121 EVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDK 180
            +K+I E+LD IS E+  FH   + +  +            R+T   L   +V GRD ++
Sbjct: 121 RMKEIMEKLDAISEERRKFHFLEKITERQ-------AAAATRETGFVLTEPKVYGRDKEE 180

Query: 181 MHL-KSLLLDMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFD 240
             + K L+ ++NV   +    I+GMGG+GKTTLA+ ++ND  V+  F  KIW+ VS+ FD
Sbjct: 181 DEIVKILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFD 240

Query: 241 AKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKPLL 300
            K +++ II +   S P+V+++   Q+ LQE++  K+YLLV+DD+WN+  EKW  L+ +L
Sbjct: 241 EKRLIKTIIGNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL 300

Query: 301 MGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLI 360
             GARG+ I+ T R  KV + +G +   + L  L  +DS  LF + AF   KE ANPNL+
Sbjct: 301 TVGARGASILATTRLEKVGSIMGTLQP-YHLSNLSPHDSLLLFMQRAFGQQKE-ANPNLV 360

Query: 361 QLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNEL 401
            +GKEI+ KCGGVPL  + +GGLL  K  E EW   ++NE+
Sbjct: 361 AIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEI 379

BLAST of Lag0002751 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 257.7 bits (657), Expect = 2.2e-67
Identity = 148/401 (36.91%), Postives = 231/401 (57.61%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAE FL  L    L  LT      L ++ G + +  KL S  S IQAVL DA+E+  K  
Sbjct: 1   MAEAFLQVL----LDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAR 120
           ++K+WL+KL  A Y+ DD+LD+   EA            KQ  +       + F Y++ +
Sbjct: 61  AIKNWLQKLNVAAYEVDDILDDCKTEA---------ARFKQAVLGRYHPRTITFCYKVGK 120

Query: 121 EVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDK 180
            +K++ E+LD I+ E+ NFHL            +  +   +R T   L   +V GR+ ++
Sbjct: 121 RMKEMMEKLDAIAEERRNFHLDER---------IIERQAARRQTGFVLTEPKVYGREKEE 180

Query: 181 MHL-KSLLLDMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFD 240
             + K L+ +++    V  + I+GMGG+GKTTLA+ ++ND  ++  F LKIW+ VS+ FD
Sbjct: 181 DEIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFD 240

Query: 241 AKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKPLL 300
            K +++ I+ES  G      ++ PLQ+ LQE++  K+Y LV+DD+WNE  EKW++L+ +L
Sbjct: 241 EKRLIKAIVESIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVL 300

Query: 301 MGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLI 360
             GA G+ I+IT R  K+ + +G +  L+ L  L + D W LF++ AF    E  +P L+
Sbjct: 301 KIGASGASILITTRLEKIGSIMGTL-QLYQLSNLSQEDCWLLFKQRAFCHQTE-TSPKLM 360

Query: 361 QLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNEL 401
           ++GKEI+ KCGGVPL  + +GGLL  K  E EW   +++E+
Sbjct: 361 EIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEI 377

BLAST of Lag0002751 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 4.2e-66
Identity = 150/403 (37.22%), Postives = 234/403 (58.06%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAE F+  L    L  LTS     L +L G +D+  +L S  S IQAVL DA+E+   + 
Sbjct: 1   MAEAFIQVL----LDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNK 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAR 120
            L++WL+KL  A Y+ DD+LDE   +A        G+   +V         + F +++ +
Sbjct: 61  PLENWLQKLNAATYEVDDILDEYKTKATRFSQSEYGRYHPKV---------IPFRHKVGK 120

Query: 121 EVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDK 180
            + ++ ++L  I+ E+ NFHL         H  +  +   +R+T S L   +V GRD +K
Sbjct: 121 RMDQVMKKLKAIAEERKNFHL---------HEKIVERQAVRRETGSVLTEPQVYGRDKEK 180

Query: 181 MHL-KSLLLDMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFD 240
             + K L+ +++   ++S + I+GMGG+GKTTLA+ ++ND  V+  F  KIWI VSE FD
Sbjct: 181 DEIVKILINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFD 240

Query: 241 AKIVVEKIIESATGSKPNVQEMD--PLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKP 300
            K +++ I+ES  G +P + EMD  PLQ+ LQE++  K+YLLV+DD+WNE  +KW +L+ 
Sbjct: 241 EKRLIKAIVESIEG-RPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRA 300

Query: 301 LLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPN 360
           +L  GA G+ ++ T R  KV + +G +   + L  L + D W LF + AF   +E  NPN
Sbjct: 301 VLKVGASGASVLTTTRLEKVGSIMGTLQP-YELSNLSQEDCWLLFMQRAF-GHQEEINPN 360

Query: 361 LIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNEL 401
           L+ +GKEI+ K GGVPL  + +GG+L  K  E  W   +++ +
Sbjct: 361 LVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPI 378

BLAST of Lag0002751 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 9.6e-63
Identity = 141/401 (35.16%), Postives = 227/401 (56.61%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAE F+      +L  LTS     L +L G +D+  +L S  S IQAVL DA+E+     
Sbjct: 1   MAEAFIQV----VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDK 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAR 120
            L++WL+KL  A Y+ DD+LDE   +A        G+   +V         + F +++ +
Sbjct: 61  PLENWLQKLNAATYEVDDILDEYKTKATRFLQSEYGRYHPKV---------IPFRHKVGK 120

Query: 121 EVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDK 180
            + ++ ++L+ I+ E+  FHL  +         +  Q  T R+T S L   +V GRD +K
Sbjct: 121 RMDQVMKKLNAIAEERKKFHLQEKI--------IERQAAT-RETGSVLTEPQVYGRDKEK 180

Query: 181 MHLKSLLLDMNVEG-NVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFD 240
             +  +L++   +   +S + I+GMGG+GKTTL++ ++ND  V+  F  KIWI +S+ F+
Sbjct: 181 DEIVKILINTASDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFN 240

Query: 241 AKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKPLL 300
            K +++ I+ES  G   +  ++ PLQ+ LQE++  K+Y LV+DD+WNE   KW +L+ +L
Sbjct: 241 EKRLIKAIVESIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVL 300

Query: 301 MGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLI 360
             GA G+ ++ T R  KV + +G +   + L  L   D W LF + AF   +E  NPNL+
Sbjct: 301 KVGASGAFVLTTTRLEKVGSIMGTLQP-YELSNLSPEDCWFLFMQRAF-GHQEEINPNLM 360

Query: 361 QLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNEL 401
            +GKEI+ KCGGVPL  + +GG+L  K  E EW   +++ +
Sbjct: 361 AIGKEIVKKCGGVPLAAKTLGGILRFKREEREWEHVRDSPI 377

BLAST of Lag0002751 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.8e-46
Identity = 123/401 (30.67%), Postives = 215/401 (53.62%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDD-LNKLESNVSAIQAVLLDAEEQPTKS 60
           + E FL+     +   L S+  +       L ++ L +L + +  I AVL+DAEE+   +
Sbjct: 4   IGEMFLAAFLQALFQTLVSEPFRSFFKRRELNENLLERLSTALLTITAVLIDAEEKQITN 63

Query: 61  HSLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAF----N 120
             ++ W+ +L D +Y A+D LD+++ EAL   +     ++ ++R    R +   F    +
Sbjct: 64  PVVEKWVNELRDVVYHAEDALDDIATEALRLNIGAESSSSNRLRQLRGRMSLGDFLDGNS 123

Query: 121 YRMAREVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKR-DTHSSLKGKEVI 180
             +   ++K+  RL+ ++S+++   L           +L+  I  +R  T S +   EV 
Sbjct: 124 EHLETRLEKVTIRLERLASQRNILGL----------KELTAMIPKQRLPTTSLVDESEVF 183

Query: 181 GRDDDKMHLKSLLLDMNVEGN-VSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIW 240
           GRDDDK  +   L+  N + N ++ +AIVG+GG+GKTTL++ LYND  V ++F  K+W  
Sbjct: 184 GRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAH 243

Query: 241 VSEQFDAKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEK--KYLLVMDDIWNESGEK 300
           VSE+FD   + +K+ ES T       ++D LQ  L+E +      +LLV+DD+WNE+   
Sbjct: 244 VSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFAD 303

Query: 301 WEDLKPLLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGK 360
           W+ L+   +  A+GS+I++T R  +VA+ I     +  L+ L + D W LF +  F + +
Sbjct: 304 WDLLRQPFIHAAQGSQILVTTRSQRVAS-IMCAVHVHNLQPLSDGDCWSLFMKTVFGNQE 363

Query: 361 EPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEW 393
              N  +  L + I+ KC G+PL ++ +GG+L  +    EW
Sbjct: 364 PCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEW 393

BLAST of Lag0002751 vs. ExPASy TrEMBL
Match: A0A6J1D168 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111016078 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 3.7e-142
Identity = 273/407 (67.08%), Postives = 321/407 (78.87%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEE--QPTK 60
           MAEG L  +A EILMKL S ASQRL M+ G+++DL KL S VS I+ VLLDAEE  Q   
Sbjct: 1   MAEGVLFHVAGEILMKLGSIASQRLGMVRGVENDLTKLTSTVSVIKNVLLDAEEHCQTKT 60

Query: 61  SHSLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRM 120
            H+L+DWL+KL +ALYDA+DVLDELS E LHREV+T GKNAKQVRIFFS SNQ+AFNYR+
Sbjct: 61  DHTLQDWLQKLEEALYDAEDVLDELSSETLHREVVTKGKNAKQVRIFFSGSNQIAFNYRV 120

Query: 121 AREVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDD 180
           AR +KKI ERLD I +E+  F+LT    +  ++      +  +++T S    +EVIGRD 
Sbjct: 121 ARNIKKIWERLDAIDAERKQFNLTENCQQRTLYGSFDQIMMGRKETCSFPNVEEVIGRDY 180

Query: 181 DKMHLKSLLLD--MNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSE 240
           DK  LK LLLD  MNV  NVSFI I GMGGIGKTTLAK LY+D EVS  F+  IWIW+S+
Sbjct: 181 DKKKLKDLLLDINMNVRHNVSFIVIAGMGGIGKTTLAKSLYHDREVSECFDSTIWIWISD 240

Query: 241 QFDAKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLK 300
           QF+ ++VVEKIIESAT  KPNV+ M+ L  +LQEVIG KKYLLVMDD+W E+ EKW +LK
Sbjct: 241 QFEVRVVVEKIIESATKRKPNVEGMEALNSELQEVIGGKKYLLVMDDVWKETEEKWHELK 300

Query: 301 PLLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANP 360
           PLLMGGA GSKI+ITKRD+KVA EI  MT+LFTLKGL E++SW LFR+VAFK+ K+PAN 
Sbjct: 301 PLLMGGASGSKILITKRDSKVATEIESMTSLFTLKGLQESNSWSLFRKVAFKEAKDPANS 360

Query: 361 NLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLKV 404
           NLI+LGKEILAKCGGVPLVIRHIG LLYSKTSEEEW SFKNNELLKV
Sbjct: 361 NLIKLGKEILAKCGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLKV 407

BLAST of Lag0002751 vs. ExPASy TrEMBL
Match: A0A6J1GPK9 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111456321 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 5.9e-140
Identity = 265/403 (65.76%), Postives = 321/403 (79.65%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAEG L  LAA+I+ KL+S ASQ+L ML G+KD+L +L+S+VSAI+AV+LDAEE+ TKSH
Sbjct: 1   MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAR 120
            LKDWLEKL +ALYDA+DVLDE+SGEA+ R     G+ AKQVRIFFSR N +AF+Y+M R
Sbjct: 61  FLKDWLEKLKEALYDAEDVLDEISGEAVRR-----GRKAKQVRIFFSRYNPIAFDYKMTR 120

Query: 121 EVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDK 180
           ++K +RERL VI++E+ NFHLT E    +  H    QI    +THS    +EVIGRDD K
Sbjct: 121 QIKNVRERLGVIAAERKNFHLT-EIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHK 180

Query: 181 MHLKSLLLDMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFDA 240
             +K LL DMNV+ N+SFIAIVGM GIGKTTLA+ L+ND++VS  F+LKIW+WVS+ F+ 
Sbjct: 181 RVIKDLLQDMNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEV 240

Query: 241 KIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKPLLM 300
           KIVVEKIIESAT S+PNV  M+ LQ  LQ+++  KKYLLVMDDIWNES +KW +LK LLM
Sbjct: 241 KIVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLM 300

Query: 301 GGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQ 360
           GGARGSKIMITKRD+ +A EI  MT+L TLKGL E+DSW LF++VAF DG EP NP LIQ
Sbjct: 301 GGARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQ 360

Query: 361 LGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLKV 404
           LGKEIL KCGGVPLVIRHIG LLY + SEEEW SFKNN+LL+V
Sbjct: 361 LGKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEV 397

BLAST of Lag0002751 vs. ExPASy TrEMBL
Match: A0A6J1DP92 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111022938 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.3e-139
Identity = 267/403 (66.25%), Postives = 321/403 (79.65%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAEG L  +A EILMKL+S+ASQ+L ML GLK+DL KL ++VS I+A+LLDAEE+ TKSH
Sbjct: 1   MAEGILFHVAGEILMKLSSEASQQLGMLWGLKNDLTKLTNSVSTIKALLLDAEERQTKSH 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAR 120
           S+KDWLEKL +ALYDADDVL+E S E+  RE MT  K  KQVRIFFS+SNQ+AF+YRMAR
Sbjct: 61  SVKDWLEKLKEALYDADDVLNEFSYESRRREGMTREKMVKQVRIFFSKSNQIAFDYRMAR 120

Query: 121 EVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDK 180
           ++K +RERLD I+++ + FH + +  +   H  L  QI  + DT S +  +EVIGRDDDK
Sbjct: 121 KIKHVRERLDSIAADNNQFHFSEKLEQKAQHVSLD-QIRERDDTSSFINEEEVIGRDDDK 180

Query: 181 MHLKSLLLDMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFDA 240
             +K+ L+DMNV+ N+SFI IVG+GG+GKTTLAK LYND +VS  FELK+WI VS QFD 
Sbjct: 181 KVVKNFLVDMNVKDNISFITIVGIGGMGKTTLAKSLYNDRQVSECFELKMWICVSYQFDV 240

Query: 241 KIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKPLLM 300
           K +VEKIIESAT S P V  M+ LQ  L++VI  KKYLLVMDDIWNES EKW  LK LL+
Sbjct: 241 KTIVEKIIESATNSNPKVGGMESLQIQLKKVIQGKKYLLVMDDIWNESEEKWFHLKNLLI 300

Query: 301 GGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQ 360
           GGARGSKIMITKRD+K   EI  +TTLFTLKGL E++SW LFR+VAFK+GKEP N NLIQ
Sbjct: 301 GGARGSKIMITKRDSKRVLEIANLTTLFTLKGLSESNSWSLFRKVAFKEGKEPTNLNLIQ 360

Query: 361 LGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLKV 404
           LGKEIL KC GVPLVIRHIG +LYSKTSEEEW SF+NNELL++
Sbjct: 361 LGKEILVKCRGVPLVIRHIGRMLYSKTSEEEWVSFQNNELLEL 402

BLAST of Lag0002751 vs. ExPASy TrEMBL
Match: A0A6J1JVS7 (putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC111488319 PE=4 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.8e-133
Identity = 256/390 (65.64%), Postives = 310/390 (79.49%), Query Frame = 0

Query: 14  LMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSHSLKDWLEKLGDAL 73
           L KL+S ASQ+L ML G+KD+L +L+S+VSAI+AV+LDAEEQ TKSH LKDWLEKL +AL
Sbjct: 233 LKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEEQ-TKSHFLKDWLEKLKEAL 292

Query: 74  YDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAREVKKIRERLDVIS 133
           YDA+DVLDE+S EA+ R     G+ AKQVRIFFSR N +AF+Y+M  ++K +RERL+VI+
Sbjct: 293 YDAEDVLDEISCEAVRR-----GRKAKQVRIFFSRYNPIAFDYKMTCQIKNVRERLEVIA 352

Query: 134 SEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDKMHLKSLLLDMNVE 193
           +E+ NFHLT E    +  H    QI    +THS    +EVIGRDD K  +K LL DMNV+
Sbjct: 353 AERKNFHLT-EIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKRVIKDLLQDMNVK 412

Query: 194 GNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFDAKIVVEKIIESATG 253
            N+SFIAIVGM GIGKTTLA+ L+ND++VS  F+LKIW+WVS+ F+ KIVVEKIIESAT 
Sbjct: 413 DNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEVKIVVEKIIESATN 472

Query: 254 SKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLKPLLMGGARGSKIMITKR 313
           S+PNV  M+ LQ  LQ+++  KKYLLVMDDIWNES +KW +LK LLMGGARGSKIMITKR
Sbjct: 473 SRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLMGGARGSKIMITKR 532

Query: 314 DNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVP 373
           D+ +A EI  MT+L TLKGL E+DSW LF++VAF DG EP NP LIQLGKEIL KCGGVP
Sbjct: 533 DSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQLGKEILVKCGGVP 592

Query: 374 LVIRHIGGLLYSKTSEEEWTSFKNNELLKV 404
           LVIRHIG LLY + SEEEW SFKNN+LL+V
Sbjct: 593 LVIRHIGRLLYIQKSEEEWISFKNNQLLEV 615

BLAST of Lag0002751 vs. ExPASy TrEMBL
Match: A0A6J1DHT2 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111021211 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 3.5e-124
Identity = 251/408 (61.52%), Postives = 306/408 (75.00%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAEG L  +A  ILMKL S  SQ + M+ GL+DDL KL + VSAI+ VL+DAEE  TK+H
Sbjct: 45  MAEGVLVHVAGNILMKLGSITSQMVGMVPGLEDDLTKLTATVSAIKNVLVDAEECQTKTH 104

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREV-MTGGKNAKQVRIFFSRSNQLAFNYRMA 120
           +L++WL+KL +  YD +D+LDE S EAL RE+  T  KN K+VRIFFS SN ++FNYRM 
Sbjct: 105 ALQNWLQKLEEVFYDVEDMLDEFSYEALRRELTTTREKNTKKVRIFFSGSNLISFNYRMT 164

Query: 121 REVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKR-DTHSSLKGKEVIGRDD 180
            ++K I ERLD + +EK+ FHL       R ++    QI  +R +T S    +EVIGRDD
Sbjct: 165 CKMKNIWERLDAVDAEKEQFHL-------RENYGSFDQITMERNETCSFSNEEEVIGRDD 224

Query: 181 DKMHLKSLLL--DMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSE 240
           DK  +K LLL  DMNV  NV F+ IVGM GIGKTTLAK LYND++VS  F+ +IWIWVS 
Sbjct: 225 DKKKVKHLLLDMDMNVMHNVLFVMIVGMDGIGKTTLAKSLYNDDDVSKCFDSRIWIWVSG 284

Query: 241 QFDAKIVVEKIIESATGS--KPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKWED 300
           QF+ K++V+K+IESATGS   PNV+ M+ L  +LQ+VI  KKY+LVMDD+WNESGEKW +
Sbjct: 285 QFEVKVIVKKMIESATGSSINPNVKGMEALLIELQKVIRGKKYMLVMDDVWNESGEKWNE 344

Query: 301 LKPLLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPA 360
           LK LLMGGARGSKI+ITKRDNKVA EI  MT+L TL+GL E+ SW LFR VAFK+G EPA
Sbjct: 345 LKHLLMGGARGSKILITKRDNKVATEIESMTSLLTLEGLRESKSWSLFRSVAFKEGNEPA 404

Query: 361 NPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNNELLK 403
           NPNLI+LGKEIL  CGGVPLVIR IG LL S TSE+EW SFK NELL+
Sbjct: 405 NPNLIKLGKEILVGCGGVPLVIRQIGRLLSSTTSEKEWMSFKENELLE 445

BLAST of Lag0002751 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 187.6 bits (475), Expect = 2.0e-47
Identity = 123/401 (30.67%), Postives = 215/401 (53.62%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDD-LNKLESNVSAIQAVLLDAEEQPTKS 60
           + E FL+     +   L S+  +       L ++ L +L + +  I AVL+DAEE+   +
Sbjct: 4   IGEMFLAAFLQALFQTLVSEPFRSFFKRRELNENLLERLSTALLTITAVLIDAEEKQITN 63

Query: 61  HSLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAF----N 120
             ++ W+ +L D +Y A+D LD+++ EAL   +     ++ ++R    R +   F    +
Sbjct: 64  PVVEKWVNELRDVVYHAEDALDDIATEALRLNIGAESSSSNRLRQLRGRMSLGDFLDGNS 123

Query: 121 YRMAREVKKIRERLDVISSEKDNFHLTNETSRSRIHHDLSHQIGTKR-DTHSSLKGKEVI 180
             +   ++K+  RL+ ++S+++   L           +L+  I  +R  T S +   EV 
Sbjct: 124 EHLETRLEKVTIRLERLASQRNILGL----------KELTAMIPKQRLPTTSLVDESEVF 183

Query: 181 GRDDDKMHLKSLLLDMNVEGN-VSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIW 240
           GRDDDK  +   L+  N + N ++ +AIVG+GG+GKTTL++ LYND  V ++F  K+W  
Sbjct: 184 GRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAH 243

Query: 241 VSEQFDAKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEK--KYLLVMDDIWNESGEK 300
           VSE+FD   + +K+ ES T       ++D LQ  L+E +      +LLV+DD+WNE+   
Sbjct: 244 VSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFAD 303

Query: 301 WEDLKPLLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGK 360
           W+ L+   +  A+GS+I++T R  +VA+ I     +  L+ L + D W LF +  F + +
Sbjct: 304 WDLLRQPFIHAAQGSQILVTTRSQRVAS-IMCAVHVHNLQPLSDGDCWSLFMKTVFGNQE 363

Query: 361 EPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEW 393
              N  +  L + I+ KC G+PL ++ +GG+L  +    EW
Sbjct: 364 PCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEW 393

BLAST of Lag0002751 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 167.5 bits (423), Expect = 2.1e-41
Identity = 115/407 (28.26%), Postives = 201/407 (49.39%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDD--LNKLESNVSAIQAVLLDAEEQPTK 60
           MA  +LS+  A ++++  + + + +E+  G      L +L+  +     VL DA+++   
Sbjct: 1   MANSYLSS-CANVMVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEH 60

Query: 61  SHSLKDWLEKLGDALYDADDVLDELSGEALHREVM--TGGKNAKQVRIFFSRSNQLAFNY 120
              +K WL  + DA + A+D+LDEL  EAL R V+   GG       +   R    A   
Sbjct: 61  VREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGRE---AIQK 120

Query: 121 RMAREVKKIRERLD--VISSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVI 180
           ++  +++K+   L+  V   E       +ET   +            R     L    ++
Sbjct: 121 KIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQ-------ASRSRPDDLPQGRLV 180

Query: 181 GRDDDKMHLKSLLL--DMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWI 240
           GR +DK+ L +LLL  D    G  + I++VGM G+GKTTL + ++ND  V+  FE+K+WI
Sbjct: 181 GRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWI 240

Query: 241 WVSEQFDAKIVVEKIIESATGSKPNVQEMDPLQRDLQEVIGEKKYLLVMDDIWNESGEKW 300
                F+   V + +++  T S  N +++  LQ  L++ +  K++LLV+DD W+ES  +W
Sbjct: 241 SAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEW 300

Query: 301 EDLKPLLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKD-GK 360
           E  +        GSKI++T R +++ + + +   ++ +K +   + W L    AF +   
Sbjct: 301 ESFQVAFTDAEEGSKIVLTTR-SEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISV 360

Query: 361 EPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNN 399
              N  L  +GK I  +C G+PL  R I   L SK + ++W +   N
Sbjct: 361 GSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKN 395

BLAST of Lag0002751 vs. TAIR 10
Match: AT5G48620.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 154.1 bits (388), Expect = 2.4e-37
Identity = 111/405 (27.41%), Postives = 208/405 (51.36%), Query Frame = 0

Query: 1   MAEGFLSTLAAEILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSH 60
           MAEGF+S    E L  L S+ S+RL+   G+ + L+ L+  + ++Q++L DA+ +   S 
Sbjct: 1   MAEGFVS-FGLEKLWDLLSRESERLQ---GIDEQLDGLKRQLRSLQSLLKDADAKKHGSD 60

Query: 61  SLKDWLEKLGDALYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAR 120
            ++++LE + D ++DA+D+++      L  E     K+ +++  F      L   +++A 
Sbjct: 61  RVRNFLEDVKDLVFDAEDIIESYVLNKLRGEGKGVKKHVRRLARF------LTDRHKVAS 120

Query: 121 EVKKIRERLDVISSEKDNFHLTN--ETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDD 180
           +++ I +R+  +  E  +F +    +  RS    +        R T+      +++G + 
Sbjct: 121 DIEGITKRISDVIGEMQSFGIQQIIDGVRSLSLQERQRVQREIRQTYPDSSESDLVGVEQ 180

Query: 181 DKMHLKSLLLDMNVEGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQF 240
               L   L++ ++      ++I GMGGIGKTTLA+ +++ + V   F+   W+ VS+QF
Sbjct: 181 SVEELVGHLVENDI---YQVVSIAGMGGIGKTTLARQVFHHDLVRRHFDGFAWVCVSQQF 240

Query: 241 DAKIVVEKIIESATGSKPNVQEMD--PLQRDLQEVIGEKKYLLVMDDIWNESGEKWEDLK 300
             K V ++I++       N+ +MD   LQ  L +++   +YLLV+DD+W +  E W+ +K
Sbjct: 241 TLKHVWQRILQELQPHDGNILQMDESALQPKLFQLLETGRYLLVLDDVWKK--EDWDRIK 300

Query: 301 PLLMGGARGSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEP--- 360
             +    RG K+++T R+  V          F    L+  +SW L   + F    E    
Sbjct: 301 -AVFPRKRGWKMLLTSRNEGVGIHADPTCLTFRASILNPEESWKLCERIVFPRRDETEVR 360

Query: 361 ANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNN 399
            +  +  +GKE++  CGG+PL ++ +GGLL +K +  EW    +N
Sbjct: 361 LDEEMEAMGKEMVTHCGGLPLAVKALGGLLANKHTVPEWKRVSDN 389

BLAST of Lag0002751 vs. TAIR 10
Match: AT3G46730.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 151.0 bits (380), Expect = 2.1e-36
Identity = 110/402 (27.36%), Postives = 197/402 (49.00%), Query Frame = 0

Query: 13  ILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSHSLKDWLEKLGDA 72
           +L K+       +  L G+KDDL +L++ ++ I   L D E +  +    K+W + + D 
Sbjct: 9   VLNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEAREREDEVSKEWTKLVLDI 68

Query: 73  LYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAREVKKIRERLDVI 132
            YD +DVLD    +   R +  G      +    ++  +    Y +  +++ ++ R+  I
Sbjct: 69  AYDIEDVLDTYFLKLEERSLRRG------LLRLTNKIGKKRDAYNIVEDIRTLKRRILDI 128

Query: 133 SSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDKMHLKSLLLDMNV 192
           + +++ F +    +  R  +  + ++   R      + + V+G +DD   L   LL  N 
Sbjct: 129 TRKRETFGI-GSFNEPRGENITNVRVRQLRRAPPVDQEELVVGLEDDVKILLVKLLSDNE 188

Query: 193 EGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFDAKIVVEKIIES-A 252
           +     I+I GMGG+GKT LA+ LYN  +V   F+ + W +VS+++  + ++ +II S  
Sbjct: 189 KDKSYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILIRIIRSLG 248

Query: 253 TGSKPNVQEMDPLQRD------LQEVIGEKKYLLVMDDIWNESGEKWEDLKPLLMGGARG 312
             S   ++++   + D      L  ++  K Y++V+DD+W+   + WE LK  L    RG
Sbjct: 249 IVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVWDP--DAWESLKRALPCDHRG 308

Query: 313 SKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEI 372
           SK++IT R   +A  +        L+ L   +SW LF   AF +  E  + +L + GKE+
Sbjct: 309 SKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFERKAFSN-IEKVDEDLQRTGKEM 368

Query: 373 LAKCGGVPLVIRHIGGLLYSKTSEE-------EWTSFKNNEL 401
           + KCGG+PL I  + GLL  K + E        W   K+N +
Sbjct: 369 VKKCGGLPLAIVVLSGLLSRKRTNEWHEVCASLWRRLKDNSI 400

BLAST of Lag0002751 vs. TAIR 10
Match: AT3G46530.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 150.6 bits (379), Expect = 2.7e-36
Identity = 110/394 (27.92%), Postives = 197/394 (50.00%), Query Frame = 0

Query: 13  ILMKLTSQASQRLEMLSGLKDDLNKLESNVSAIQAVLLDAEEQPTKSHSLKDWLEKLGDA 72
           ++ K+ +   +   M   +K+DL +L++ ++ I   L D E +  +    K+W + + D 
Sbjct: 9   VVGKIGNYLIEEASMFMAVKEDLEELKTELTCIHGYLKDVEAREREDEVSKEWSKLVLDF 68

Query: 73  LYDADDVLDELSGEALHREVMTGGKNAKQVRIFFSRSNQLAFNYRMAREVKKIRERLDVI 132
            YD +DVLD    +   R    G      +R   ++  +    Y +  +++ ++ R+  I
Sbjct: 69  AYDVEDVLDTYHLKLEERSQRRG------LRRLTNKIGRKMDAYSIVDDIRILKRRILDI 128

Query: 133 SSEKDNFHLTNETSRSRIHHDLSHQIGTKRDTHSSLKGKEVIGRDDDKMHLKSLLLDMNV 192
           + +++ + +          +  S ++   R   S  + + V+G +DD   L   LLD   
Sbjct: 129 TRKRETYGIGGLKEPQGGGNTSSLRVRQLRRARSVDQEEVVVGLEDDAKILLEKLLDYE- 188

Query: 193 EGNVSFIAIVGMGGIGKTTLAKFLYNDNEVSNFFELKIWIWVSEQFDAKIVVEKIIESAT 252
           E N   I+I GMGG+GKT LA+ LYN  +V   FE + W +VS+++    ++ +II S  
Sbjct: 189 EKNRFIISIFGMGGLGKTALARKLYNSRDVKERFEYRAWTYVSQEYKTGDILMRIIRSL- 248

Query: 253 GSKPNVQEMDPLQRDLQE--------VIGEKKYLLVMDDIWNESGEKWEDLKPLLMGGAR 312
               + +E++ +++  +E        ++  KKYL+V+DDIW    E W+ LK  L     
Sbjct: 249 -GMTSGEELEKIRKFAEEELEVYLYGLLEGKKYLVVVDDIWER--EAWDSLKRALPCNHE 308

Query: 313 GSKIMITKRDNKVAAEIGRMTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKE 372
           GS+++IT R   VA  +        L+ L   +SW LF + AF++  +  + +L++ GKE
Sbjct: 309 GSRVIITTRIKAVAEGVDGRFYAHKLRFLTFEESWELFEQRAFRN-IQRKDEDLLKTGKE 368

Query: 373 ILAKCGGVPLVIRHIGGLLYSKTSEEEWTSFKNN 399
           ++ KC G+PL I  + GLL  KT   EW    N+
Sbjct: 369 MVQKCRGLPLCIVVLAGLLSRKT-PSEWNDVCNS 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897566.14.3e-14568.64putative disease resistance protein RGA4 [Benincasa hispida][more]
XP_022147051.17.6e-14267.08putative disease resistance protein RGA4 [Momordica charantia][more]
XP_022953917.11.2e-13965.76putative disease resistance protein RGA4 [Cucurbita moschata][more]
XP_031745107.11.6e-13966.50putative disease resistance protein RGA4 [Cucumis sativus][more]
KAE8646395.11.6e-13966.50hypothetical protein Csa_016171 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q7XA391.6e-7038.90Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA402.2e-6736.91Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XBQ94.2e-6637.22Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA429.6e-6335.16Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LRR42.8e-4630.67Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1D1683.7e-14267.08putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1GPK95.9e-14065.76putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1DP921.3e-13966.25putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1JVS71.8e-13365.64putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1DHT23.5e-12461.52putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G14470.12.0e-4730.67NB-ARC domain-containing disease resistance protein [more]
AT3G14460.12.1e-4128.26LRR and NB-ARC domains-containing disease resistance protein [more]
AT5G48620.12.4e-3727.41Disease resistance protein (CC-NBS-LRR class) family [more]
AT3G46730.12.1e-3627.36NB-ARC domain-containing disease resistance protein [more]
AT3G46530.12.7e-3627.92NB-ARC domain-containing disease resistance protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 28..55
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 369..383
score: 37.83
coord: 198..213
score: 58.59
coord: 271..285
score: 38.67
NoneNo IPR availableGENE3D1.20.5.4130coord: 11..130
e-value: 3.0E-20
score: 74.3
NoneNo IPR availablePANTHERPTHR23155:SF1132OS08G0543100 PROTEINcoord: 7..394
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 332..402
e-value: 4.9E-12
score: 47.3
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 13..97
e-value: 1.8E-20
score: 73.0
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 187..397
e-value: 3.9E-46
score: 157.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 156..326
e-value: 1.7E-36
score: 127.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 161..400
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 7..394
IPR038005Virus X resistance protein-like, coiled-coil domainCDDcd14798RX-CC_likecoord: 2..134
e-value: 4.11131E-13
score: 63.7951

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002751.1Lag0002751.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding