Lag0002750 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002750
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptiondisease resistance protein RGA2
Locationchr4: 45313307 .. 45314758 (+)
RNA-Seq ExpressionLag0002750
SyntenyLag0002750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTACGTTGTTCACTTTAAAAGGCTTACATGAAAACGATTCTTGGCCATTGTTTAGAGAAGTGGCATTCAAAGATGGCAAAGAGCCAGCAAATCCAAACTTGATACAATTGGGAAAAGAAATTTTAGCAAAATGTGGAGGTGTTCCTCTTGTAATAAGACACATAGGAGGCTTGTTATACTCTAAAACTTCGGAAGAAGAGTGGATGTCCTTCAAGAATAATGAACTTTTGAAAGTCACTCAAGAAAACAATGATATGACATCCATATTAGAATTGAGCTATAACCATCTCCCAACACATTTGAAGCAATGTTTTGCCTATTCATCCTTGTTTCCCAAAGGAAATAAAATAGAGATAAATGAATTGATTAAACAATGGATAGCTCAAGGTTTTATCAAATTGTCAAATGGAGGTAAATCCATGGAAGATATAGGAAAGGAGTATTTCAAGGAATTATGTTCGAGGTTTTTTTATGAAAATTCTAGTGATGAGTATAACTTTGATGATTGTGTTCATATGCATGATGTGATATGTGAGCTTACAATGAAGGTAGCAGGAAAGAAATTCTATGTACGTGGAAATCTCAATAATGATTATGTTGTCAGTGAACAAACTCGTCATATTTCATTTGACTATGTAATAATATCTTGGAGGGATGTTCTATCCAAATTACGCAAGGCTAAAGGATTAAGAACGTTTCTTTTATTACATTCTTACTATGCCAACAAGAATAAGGTCAATAAAACTATCTTGGATGAACTATTTTCCAGTTTTCCACATTTGCGAGTATTAAGTTTGTATGCCTCGTATATTTATGAAGTGCCCAATTCTATAAGAAAGCTTAGACAGCTTCGATATTTAGATCTCTCTGAAAATTGGAGGATGGAATTACTTCCAAATTCTATCACTAGGTTACAAAATTTGCAGACACTAAAGCTAACAAGCTGTTCTGGACTAAAGGAATTGCCAAGGGACACTAGCAATCTTGTTAATCTTAGGCAACTTGATTTTTCTCGTCGGAGAATAAGACATATGCCGAAAGGGATGGAGAAGTTGACTTGTCTTCAAAGAATAACTTTATTTGAGTTGGATTGCAAAAGGTTGAGTAAGCCAAGTGAATTGAATAAGTTCAACAATCTAAGTACACAATCATGGAGAATCACAGGTTTAGAGCAGCTGAGATCTAGTCCATCTGAAGTTAGCTCAGTAAATCTAAAAGGCGGAAAAGATTTGCGAGATTTGACACTGGAATGGAAATTGAGCCAAGAAGAATGCGAAGGTGCGTCTGATGAAAGAATAATGGAAGGGTTAGAACCACATCCAAATGTTGAAATCTTGAGCATTGACGGGTACAGTGGAGTAGGATTACCCAATTGGGTGTCTTGCTCGCTCTTGATGTTAACTGAAATTACAATTGCAAATTGCCATAGATTGCAACATCTACCTTAG

mRNA sequence

ATGACTACGTTGTTCACTTTAAAAGGCTTACATGAAAACGATTCTTGGCCATTGTTTAGAGAAGTGGCATTCAAAGATGGCAAAGAGCCAGCAAATCCAAACTTGATACAATTGGGAAAAGAAATTTTAGCAAAATGTGGAGGTGTTCCTCTTGTAATAAGACACATAGGAGGCTTGTTATACTCTAAAACTTCGGAAGAAGAGTGGATGTCCTTCAAGAATAATGAACTTTTGAAAGTCACTCAAGAAAACAATGATATGACATCCATATTAGAATTGAGCTATAACCATCTCCCAACACATTTGAAGCAATGTTTTGCCTATTCATCCTTGTTTCCCAAAGGAAATAAAATAGAGATAAATGAATTGATTAAACAATGGATAGCTCAAGGTTTTATCAAATTGTCAAATGGAGGTAAATCCATGGAAGATATAGGAAAGGAGTATTTCAAGGAATTATGTTCGAGGTTTTTTTATGAAAATTCTAGTGATGAGTATAACTTTGATGATTGTGTTCATATGCATGATGTGATATGTGAGCTTACAATGAAGGTAGCAGGAAAGAAATTCTATGTACGTGGAAATCTCAATAATGATTATGTTGTCAGTGAACAAACTCGTCATATTTCATTTGACTATGTAATAATATCTTGGAGGGATGTTCTATCCAAATTACGCAAGGCTAAAGGATTAAGAACGTTTCTTTTATTACATTCTTACTATGCCAACAAGAATAAGGTCAATAAAACTATCTTGGATGAACTATTTTCCAGTTTTCCACATTTGCGAGTATTAAGTTTGTATGCCTCGTATATTTATGAAGTGCCCAATTCTATAAGAAAGCTTAGACAGCTTCGATATTTAGATCTCTCTGAAAATTGGAGGATGGAATTACTTCCAAATTCTATCACTAGGTTACAAAATTTGCAGACACTAAAGCTAACAAGCTGTTCTGGACTAAAGGAATTGCCAAGGGACACTAGCAATCTTGTTAATCTTAGGCAACTTGATTTTTCTCGTCGGAGAATAAGACATATGCCGAAAGGGATGGAGAAGTTGACTTGTCTTCAAAGAATAACTTTATTTGAGTTGGATTGCAAAAGGTTGAGTAAGCCAAGTGAATTGAATAAGTTCAACAATCTAAGTACACAATCATGGAGAATCACAGGTTTAGAGCAGCTGAGATCTAGTCCATCTGAAGTTAGCTCAGTAAATCTAAAAGGCGGAAAAGATTTGCGAGATTTGACACTGGAATGGAAATTGAGCCAAGAAGAATGCGAAGGTGCGTCTGATGAAAGAATAATGGAAGGGTTAGAACCACATCCAAATGTTGAAATCTTGAGCATTGACGGGTACAGTGGAGTAGGATTACCCAATTGGGTGTCTTGCTCGCTCTTGATGTTAACTGAAATTACAATTGCAAATTGCCATAGATTGCAACATCTACCTTAG

Coding sequence (CDS)

ATGACTACGTTGTTCACTTTAAAAGGCTTACATGAAAACGATTCTTGGCCATTGTTTAGAGAAGTGGCATTCAAAGATGGCAAAGAGCCAGCAAATCCAAACTTGATACAATTGGGAAAAGAAATTTTAGCAAAATGTGGAGGTGTTCCTCTTGTAATAAGACACATAGGAGGCTTGTTATACTCTAAAACTTCGGAAGAAGAGTGGATGTCCTTCAAGAATAATGAACTTTTGAAAGTCACTCAAGAAAACAATGATATGACATCCATATTAGAATTGAGCTATAACCATCTCCCAACACATTTGAAGCAATGTTTTGCCTATTCATCCTTGTTTCCCAAAGGAAATAAAATAGAGATAAATGAATTGATTAAACAATGGATAGCTCAAGGTTTTATCAAATTGTCAAATGGAGGTAAATCCATGGAAGATATAGGAAAGGAGTATTTCAAGGAATTATGTTCGAGGTTTTTTTATGAAAATTCTAGTGATGAGTATAACTTTGATGATTGTGTTCATATGCATGATGTGATATGTGAGCTTACAATGAAGGTAGCAGGAAAGAAATTCTATGTACGTGGAAATCTCAATAATGATTATGTTGTCAGTGAACAAACTCGTCATATTTCATTTGACTATGTAATAATATCTTGGAGGGATGTTCTATCCAAATTACGCAAGGCTAAAGGATTAAGAACGTTTCTTTTATTACATTCTTACTATGCCAACAAGAATAAGGTCAATAAAACTATCTTGGATGAACTATTTTCCAGTTTTCCACATTTGCGAGTATTAAGTTTGTATGCCTCGTATATTTATGAAGTGCCCAATTCTATAAGAAAGCTTAGACAGCTTCGATATTTAGATCTCTCTGAAAATTGGAGGATGGAATTACTTCCAAATTCTATCACTAGGTTACAAAATTTGCAGACACTAAAGCTAACAAGCTGTTCTGGACTAAAGGAATTGCCAAGGGACACTAGCAATCTTGTTAATCTTAGGCAACTTGATTTTTCTCGTCGGAGAATAAGACATATGCCGAAAGGGATGGAGAAGTTGACTTGTCTTCAAAGAATAACTTTATTTGAGTTGGATTGCAAAAGGTTGAGTAAGCCAAGTGAATTGAATAAGTTCAACAATCTAAGTACACAATCATGGAGAATCACAGGTTTAGAGCAGCTGAGATCTAGTCCATCTGAAGTTAGCTCAGTAAATCTAAAAGGCGGAAAAGATTTGCGAGATTTGACACTGGAATGGAAATTGAGCCAAGAAGAATGCGAAGGTGCGTCTGATGAAAGAATAATGGAAGGGTTAGAACCACATCCAAATGTTGAAATCTTGAGCATTGACGGGTACAGTGGAGTAGGATTACCCAATTGGGTGTCTTGCTCGCTCTTGATGTTAACTGAAATTACAATTGCAAATTGCCATAGATTGCAACATCTACCTTAG

Protein sequence

MTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICELTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLPNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSRRRIRHMPKGMEKLTCLQRITLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEWKLSQEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLMLTEITIANCHRLQHLP
Homology
BLAST of Lag0002750 vs. NCBI nr
Match: XP_031745107.1 (putative disease resistance protein RGA4 [Cucumis sativus])

HSP 1 Score: 634.8 bits (1636), Expect = 6.1e-178
Identity = 327/484 (67.56%), Postives = 381/484 (78.72%), Query Frame = 0

Query: 1   MTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLL 60
           MT+LFTL+GL E++SW LF +VAFK+GKE  +P+ I LGKEIL +CGGVPLVIRH+G +L
Sbjct: 326 MTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPSTIHLGKEILVRCGGVPLVIRHVGRML 385

Query: 61  YSKTSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEI 120
           YSKTS+EEWMSFK+NELL+V Q++NDMTSIL+LSYNHLP +LK+CFAYSSLFPKG KIEI
Sbjct: 386 YSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSYNHLPPNLKRCFAYSSLFPKGYKIEI 445

Query: 121 NELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICE 180
            +LI+QW+AQGFI++SNG KS+ED GK+YF ELC RFFY NSSDE N +D V MHDV+CE
Sbjct: 446 KDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCWRFFYANSSDECNINDIVCMHDVMCE 505

Query: 181 LTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSY 240
              KVAG K YVRGN NNDYVVSEQT HISFDY I SW+DVLSKL KAKGLRT LLL   
Sbjct: 506 FVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGIQSWQDVLSKLCKAKGLRTILLLFRP 565

Query: 241 YANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLP 300
           Y   NK++K ILDELFSSFP LRVL L+ S I  VP SI+KLR LRYLDLSEN  MEL+P
Sbjct: 566 YEKMNKIDKAILDELFSSFPRLRVLDLHFSQISVVPKSIKKLRHLRYLDLSEN-DMELIP 625

Query: 301 NSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR-RRIRHMPKGMEKLTCLQRI 360
           +SI  LQNLQTL LT C  LKELPRD  NLVNLR L F     +    +GMEKLTCLQ I
Sbjct: 626 HSIIELQNLQTLNLTECYELKELPRDIDNLVNLRHLTFEPCMEVTPTSEGMEKLTCLQTI 685

Query: 361 TLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEW 420
           +LF  DCK+ +K  ELN  + L T   +I GLE+LRSSPSE++ +NLK  K  + L LEW
Sbjct: 686 SLFVFDCKKTNKLWELNDLSYL-TGELKIIGLEKLRSSPSEITLINLKDKKGWQGLNLEW 745

Query: 421 KLSQEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLMLTEITIANCHRL 480
           KL ++E EG +DE IMEGLEPHPNVE LSI+GY+G  LPNWV  SL+ LTEI I NC R+
Sbjct: 746 KLGKDEYEGEADETIMEGLEPHPNVESLSINGYTGGALPNWVFNSLMKLTEIEIENCPRV 805

Query: 481 QHLP 484
           QHLP
Sbjct: 806 QHLP 807

BLAST of Lag0002750 vs. NCBI nr
Match: KAE8646395.1 (hypothetical protein Csa_016171 [Cucumis sativus])

HSP 1 Score: 634.8 bits (1636), Expect = 6.1e-178
Identity = 327/484 (67.56%), Postives = 381/484 (78.72%), Query Frame = 0

Query: 1   MTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLL 60
           MT+LFTL+GL E++SW LF +VAFK+GKE  +P+ I LGKEIL +CGGVPLVIRH+G +L
Sbjct: 326 MTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPSTIHLGKEILVRCGGVPLVIRHVGRML 385

Query: 61  YSKTSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEI 120
           YSKTS+EEWMSFK+NELL+V Q++NDMTSIL+LSYNHLP +LK+CFAYSSLFPKG KIEI
Sbjct: 386 YSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSYNHLPPNLKRCFAYSSLFPKGYKIEI 445

Query: 121 NELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICE 180
            +LI+QW+AQGFI++SNG KS+ED GK+YF ELC RFFY NSSDE N +D V MHDV+CE
Sbjct: 446 KDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCWRFFYANSSDECNINDIVCMHDVMCE 505

Query: 181 LTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSY 240
              KVAG K YVRGN NNDYVVSEQT HISFDY I SW+DVLSKL KAKGLRT LLL   
Sbjct: 506 FVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGIQSWQDVLSKLCKAKGLRTILLLFRP 565

Query: 241 YANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLP 300
           Y   NK++K ILDELFSSFP LRVL L+ S I  VP SI+KLR LRYLDLSEN  MEL+P
Sbjct: 566 YEKMNKIDKAILDELFSSFPRLRVLDLHFSQISVVPKSIKKLRHLRYLDLSEN-DMELIP 625

Query: 301 NSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR-RRIRHMPKGMEKLTCLQRI 360
           +SI  LQNLQTL LT C  LKELPRD  NLVNLR L F     +    +GMEKLTCLQ I
Sbjct: 626 HSIIELQNLQTLNLTECYELKELPRDIDNLVNLRHLTFEPCMEVTPTSEGMEKLTCLQTI 685

Query: 361 TLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEW 420
           +LF  DCK+ +K  ELN  + L T   +I GLE+LRSSPSE++ +NLK  K  + L LEW
Sbjct: 686 SLFVFDCKKTNKLWELNDLSYL-TGELKIIGLEKLRSSPSEITLINLKDKKGWQGLNLEW 745

Query: 421 KLSQEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLMLTEITIANCHRL 480
           KL ++E EG +DE IMEGLEPHPNVE LSI+GY+G  LPNWV  SL+ LTEI I NC R+
Sbjct: 746 KLGKDEYEGEADETIMEGLEPHPNVESLSINGYTGGALPNWVFNSLMKLTEIEIENCPRV 805

Query: 481 QHLP 484
           QHLP
Sbjct: 806 QHLP 807

BLAST of Lag0002750 vs. NCBI nr
Match: XP_038897566.1 (putative disease resistance protein RGA4 [Benincasa hispida])

HSP 1 Score: 605.5 bits (1560), Expect = 3.9e-169
Identity = 327/488 (67.01%), Postives = 378/488 (77.46%), Query Frame = 0

Query: 1   MTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLL 60
           MT+LF+L+GL E+ SW LF +VAFK+GKE  NPNLIQ GKEIL KCGGVPLVIRHIG LL
Sbjct: 325 MTSLFSLEGLPESKSWSLFSKVAFKEGKELENPNLIQTGKEILMKCGGVPLVIRHIGRLL 384

Query: 61  YSKTSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEI 120
           YS+T+EEEWMSFK+NELL++ Q++NDMTS+L+LSYNHLP +LKQCFAY SLFPK   ++I
Sbjct: 385 YSRTTEEEWMSFKDNELLEIIQQDNDMTSVLKLSYNHLPPNLKQCFAYLSLFPKRKSLKI 444

Query: 121 NELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICE 180
           N+LI+QW+A GFI+ SNG KSME+ GK YFKELC RFF ENS +E NFD+ VHMHDV+ +
Sbjct: 445 NDLIRQWMALGFIETSNGSKSMEETGKGYFKELCWRFFLENSMNECNFDNDVHMHDVMRD 504

Query: 181 LTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHS- 240
           L   VAGKK YVR N+N DY  SE+TRH+SFD  I SW DVLSKL KAKGLRTF L  S 
Sbjct: 505 LATNVAGKK-YVRENINCDYAFSEKTRHVSFDCKIKSWSDVLSKLHKAKGLRTFQLFSSN 564

Query: 241 -YYANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMEL 300
             Y  KN++N+ ILDE+FSSFP LRVL L  S    VPNSIR+LR LRYLDLSEN  ME 
Sbjct: 565 FEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSPMVPNSIRRLRHLRYLDLSEN-NMES 624

Query: 301 LPNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSRRRIRHMPKGMEKLTCLQR 360
           LPNSIT L+NLQTL L  C  LKELPRDTSNLVNLR LDF+   + HMP+GM KL+CLQ 
Sbjct: 625 LPNSITELRNLQTLNLMYCGDLKELPRDTSNLVNLRHLDFNTSILTHMPEGMGKLSCLQT 684

Query: 361 ITLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLE 420
           ++ F LDCKR ++ SEL+   +L+    RI GLEQLRS  SEVS VNLK  K L+ L LE
Sbjct: 685 LSYFVLDCKRSNQLSELDVLIHLNGY-LRIIGLEQLRSE-SEVSLVNLKDIKGLKKLELE 744

Query: 421 WKLS--QEECEGASDE-RIMEGLEPHPNVEILSIDGYSGVGLPNW-VSCSLLMLTEITIA 480
           WKLS   +E EG +DE  IMEGLEPHPN+E L IDGYSGVGLPNW VS SL  LT I I 
Sbjct: 745 WKLSGDDQEYEGENDETAIMEGLEPHPNIESLHIDGYSGVGLPNWVVSTSLSKLTNIVIH 804

Query: 481 NCHRLQHL 483
            CHRLQHL
Sbjct: 805 KCHRLQHL 808

BLAST of Lag0002750 vs. NCBI nr
Match: XP_008461332.2 (PREDICTED: disease resistance protein RGA2 [Cucumis melo])

HSP 1 Score: 602.4 bits (1552), Expect = 3.3e-168
Identity = 320/487 (65.71%), Postives = 375/487 (77.00%), Query Frame = 0

Query: 1    MTTLFTLKGLHENDSWPLFREVAFKDG-KEPANPNLIQLGKEILAKCGGVPLVIRHIGGL 60
            MTT  TLKGL E+ SW LFREVAF+     P NP L  +GKEI ++CGGVPL IRH+G  
Sbjct: 582  MTTSVTLKGLSEDRSWSLFREVAFEGNLPNPINPKLELMGKEISSRCGGVPLAIRHMGRF 641

Query: 61   LYSKTSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIE 120
            L SK  EEEWMS  +++LL+VTQ+NND+ SILELSYN LP HLKQCFAYSSLFPK +K++
Sbjct: 642  LCSKRCEEEWMSSLSDKLLEVTQQNNDVASILELSYNDLPLHLKQCFAYSSLFPKVHKLK 701

Query: 121  INELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVIC 180
            +NELI+QW+AQGFI+ S G KSME IGK YF ELC RFFYENSSDE NFDD V+MHDV+ 
Sbjct: 702  VNELIRQWVAQGFIESSKGEKSMETIGKNYFDELCWRFFYENSSDENNFDDYVYMHDVMY 761

Query: 181  ELTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHS 240
            EL  KVA KK YV G++NN+YVVSEQT H SFDY I SW+DVLSKL KAKGLRTF+ LHS
Sbjct: 762  ELARKVADKKLYVCGDINNNYVVSEQTLHFSFDYKIQSWQDVLSKLCKAKGLRTFMFLHS 821

Query: 241  YYANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELL 300
             Y  KN+VN+  LDELFSSFP LRVL L  S I+ +PNSI+KL+ LRYLDLSEN  M+ L
Sbjct: 822  PYEEKNEVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSEN-NMKSL 881

Query: 301  PNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR--RRIRHM-PKGMEKLTCL 360
            PNSIT LQ+LQTLKLT C  LKE+PRD + LVNLR LD SR   +I HM P+GMEKL+CL
Sbjct: 882  PNSITELQDLQTLKLTGCYALKEMPRDINKLVNLRHLD-SRCCSQITHMAPEGMEKLSCL 941

Query: 361  QRITLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLT 420
            Q I+LF  DC + +K  ELN+ NNL T   +ITGL+QLRS+P EVS VNLK  K LR L 
Sbjct: 942  QTISLFVFDCTKTNKLRELNELNNL-TGELKITGLQQLRSTPYEVSLVNLKDKKCLRSLK 1001

Query: 421  LEWKLSQEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLMLTEITIANC 480
            LEWK+   + E  +DER+M+GLEP+PNVE L I GY+GVGLP W+    L LTEI I +C
Sbjct: 1002 LEWKMGLYKHEDEADERVMKGLEPNPNVESLGIKGYNGVGLPKWMFNCHLKLTEIEIVSC 1061

Query: 481  HRLQHLP 484
            HRLQHLP
Sbjct: 1062 HRLQHLP 1065

BLAST of Lag0002750 vs. NCBI nr
Match: KAA0058606.1 (disease resistance protein RGA2 [Cucumis melo var. makuwa])

HSP 1 Score: 594.3 bits (1531), Expect = 9.1e-166
Identity = 317/488 (64.96%), Postives = 374/488 (76.64%), Query Frame = 0

Query: 1   MTTLFTLKGLHENDSWPLFREVAFKDG-KEPANPNLIQLGKEILAKCGGVPLVIRHIGGL 60
           MTT  TLKGL E+ SW LFREVAF++   +P NP L  +GKEI + CGGVPL IRH+G  
Sbjct: 328 MTTSVTLKGLSEDSSWSLFREVAFEENLPKPINPKLELMGKEISSICGGVPLAIRHMGRF 387

Query: 61  LYSKTSEEEWMSFKN-NELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKI 120
           L SK  EEEWMS  +  + L+V Q+NND+ SILELSYN LP HLKQCFAYSSLFPKG+K+
Sbjct: 388 LCSKRCEEEWMSSLSWKKHLEVAQQNNDVASILELSYNDLPLHLKQCFAYSSLFPKGHKL 447

Query: 121 EINELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVI 180
           ++NELI+QW+AQGFI+ S G KSME IGK YF ELC RFFYENSSDE NFDD V+MHDV+
Sbjct: 448 KVNELIRQWVAQGFIESSKGEKSMETIGKNYFDELCWRFFYENSSDENNFDDYVYMHDVM 507

Query: 181 CELTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLH 240
            EL  KVA KK +V G++NN+YVVSEQT H S DY I SW+DVLSKL KAKGLRTF+ LH
Sbjct: 508 YELARKVADKKLFVCGDINNNYVVSEQTLHFSLDYKIQSWQDVLSKLCKAKGLRTFMFLH 567

Query: 241 SYYANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMEL 300
           S Y  KN+VN+  LDELFSSFP LRVL L  S I+ +PNSI+KL+ LRYLDLSEN  M+ 
Sbjct: 568 SPYEEKNEVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSEN-NMKS 627

Query: 301 LPNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR--RRIRHM-PKGMEKLTC 360
           LPNSIT LQ+LQTLKLT C  LKE+PRD + LVNLR LD SR   +I HM P+GMEKL+C
Sbjct: 628 LPNSITELQDLQTLKLTGCYALKEMPRDINKLVNLRHLD-SRCCSQITHMAPEGMEKLSC 687

Query: 361 LQRITLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDL 420
           LQ I+LF  DC + +K  ELN+ NNL T   +ITGL+QLRS+P EVS VNLKG K LR L
Sbjct: 688 LQTISLFVFDCTKTNKLRELNELNNL-TGELKITGLQQLRSTPYEVSLVNLKGKKCLRSL 747

Query: 421 TLEWKLSQEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLMLTEITIAN 480
            LEWK+   + E  +DER+M+GLEP+PNVE L I GY+GVGLP W+    L LTEI + +
Sbjct: 748 KLEWKMGLYKHEDEADERVMKGLEPNPNVESLGIKGYNGVGLPKWMFNCHLKLTEIEMVS 807

Query: 481 CHRLQHLP 484
           CHRLQHLP
Sbjct: 808 CHRLQHLP 812

BLAST of Lag0002750 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.2e-62
Identity = 155/487 (31.83%), Postives = 259/487 (53.18%), Query Frame = 0

Query: 7   LKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSE 66
           L+ L + D W LF +  F + +   N  +  L + I+ KC G+PL ++ +GG+L  +   
Sbjct: 331 LQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKV 390

Query: 67  EEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINELIKQ 126
            EW    ++ +  +  + +++  +L +SY +LP HLK+CFAY S+FPKG+  E ++++  
Sbjct: 391 IEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLL 450

Query: 127 WIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICELTMKVA 186
           W+A+GF++ +   K++E++G EYF EL SR   + +   Y       MHD I EL    +
Sbjct: 451 WMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRY------IMHDFINELAQFAS 510

Query: 187 GKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANKN- 246
           G +F  +        VSE+TR++S+     +       LR+ K LRTFL L    ++++ 
Sbjct: 511 G-EFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSC 570

Query: 247 KVNKTILDELFSSFPHLRVLSLYASYIYEV-PNSIRKLRQLRYLDLSENWRMELLPNSIT 306
            +++ + ++L  +   LRVLSL    I  + P+  + +   R+LDLS    +E LP S+ 
Sbjct: 571 CLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLC 630

Query: 307 RLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSRRRIRHMPKGMEKLTCLQRITLFEL 366
            + NLQTL L+ CS LKELP D SNL+NLR LD    ++R MP+   +L  LQ +T F +
Sbjct: 631 YMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFV 690

Query: 367 DCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEWKLSQE 426
                S+ SEL   ++L  +  +I  L+++    ++ +  NL   K LR++   W+    
Sbjct: 691 SASDGSRISELGGLHDLHGK-LKIVELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSS 750

Query: 427 ECEG-------ASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVS-CSLLMLTEITIANC 484
             E         ++  + E L PH ++E L+I+ Y G   P+W+S  S   +  I +  C
Sbjct: 751 SSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLREC 807

BLAST of Lag0002750 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 241.9 bits (616), Expect = 1.5e-62
Identity = 162/485 (33.40%), Postives = 253/485 (52.16%), Query Frame = 0

Query: 4   LFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSK 63
           L+ L  L + D W LF++ AF    E  +P L+++GKEI+ KCGGVPL  + +GGLL  K
Sbjct: 305 LYQLSNLSQEDCWLLFKQRAFCHQTE-TSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFK 364

Query: 64  TSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINEL 123
             E EW   +++E+  + Q+ N +   L LSY+HLP  L+QCFAY ++FPK  KIE   L
Sbjct: 365 REESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEYL 424

Query: 124 IKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFY---ENSSDEYNFDDCVHMHDVICE 183
           I  W+A  F+ LS G   +ED+G E + EL  R F+   E  S +  F     +HD+   
Sbjct: 425 IALWMAHSFL-LSKGNMELEDVGNEVWNELYLRSFFQEIEVKSGKTYFKMHDLIHDLATS 484

Query: 184 LTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSY 243
           +    A  +   + N+ +D             +++ +++D++S                 
Sbjct: 485 MFSASASSRSIRQINVKDDE---------DMMFIVTNYKDMMS----------------- 544

Query: 244 YANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLP 303
               ++V  +    LF  F  LRVL+L  S   ++P+S+  L  LRYLDLS N ++  LP
Sbjct: 545 -IGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGN-KICSLP 604

Query: 304 NSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSRRRIRHMPKGMEKLTCLQRIT 363
             + +LQNLQTL L +C  L  LP+ TS L +LR L      +  MP  +  LTCL+ + 
Sbjct: 605 KRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLKTLG 664

Query: 364 LFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEW- 423
            F +  ++  +  EL   N     S  IT LE++++   E    NL    +L  L++ W 
Sbjct: 665 YFVVGERKGYQLGELRNLNLRGAIS--ITHLERVKND-MEAKEANLSAKANLHSLSMSWD 724

Query: 424 KLSQEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLM-LTEITIANCHR 483
           + ++ E E   + +++E L+PHPN++ L I  + G  LP+W++ S+L  +  I I+ C  
Sbjct: 725 RPNRYESE---EVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCEN 753

BLAST of Lag0002750 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 239.2 bits (609), Expect = 9.7e-62
Identity = 157/480 (32.71%), Postives = 241/480 (50.21%), Query Frame = 0

Query: 5   FTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKT 64
           + L  L   D W LF + AF   +E  NPNL+ +GKEI+ KCGGVPL  + +GG+L  K 
Sbjct: 306 YELSNLSPEDCWFLFMQRAF-GHQEEINPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKR 365

Query: 65  SEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINELI 124
            E EW   +++ +  + Q+ + +   L LSY+HLP  L+QCF Y ++FPK  K+    LI
Sbjct: 366 EEREWEHVRDSPIWNLPQDESSILPALRLSYHHLPLDLRQCFVYCAVFPKDTKMAKENLI 425

Query: 125 KQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICELTMK 184
             W+A GF+ LS G   +ED+G E + EL  R F++    E                   
Sbjct: 426 AFWMAHGFL-LSKGNLELEDVGNEVWNELYLRSFFQEIEVE------------------- 485

Query: 185 VAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANK 244
            +GK ++   +L +D   S           + S     S +R+        ++   +A  
Sbjct: 486 -SGKTYFKMHDLIHDLATS-----------LFSANTSSSNIREINANYDGYMMSIGFA-- 545

Query: 245 NKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLPNSIT 304
            +V  +    L   F  LRVL+L  S + ++P+SI  L  LRYLDLS N+R+  LP  + 
Sbjct: 546 -EVVSSYSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLC 605

Query: 305 RLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSRRRIRHMPKGMEKLTCLQRITLFEL 364
           +LQNLQTL L  C  L  LP+ TS L +LR L      +   P  +  LTCL+ ++ F +
Sbjct: 606 KLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLSCFVI 665

Query: 365 DCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEWKLSQE 424
             ++  +  EL   N   + S  IT L++++   ++    NL    +L  L L W L  +
Sbjct: 666 GKRKGHQLGELKNLNLYGSIS--ITKLDRVKKD-TDAKEANLSAKANLHSLCLSWDLDGK 725

Query: 425 ECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLM-LTEITIANCHRLQHLP 484
                 D  ++E L+PH N++ L I+G+ G+ LP+W++ S+L  +  I I  C     LP
Sbjct: 726 H---RYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLP 743

BLAST of Lag0002750 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.2e-59
Identity = 160/481 (33.26%), Postives = 242/481 (50.31%), Query Frame = 0

Query: 5   FTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKT 64
           + L  L  +DS  LF + AF   KE ANPNL+ +GKEI+ KCGGVPL  + +GGLL  K 
Sbjct: 308 YHLSNLSPHDSLLLFMQRAFGQQKE-ANPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKR 367

Query: 65  SEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINELI 124
            E EW   ++NE+  + Q+ + +   L LSY+HLP  L+QCFAY ++FPK  K+    LI
Sbjct: 368 EESEWEHVRDNEIWSLPQDESSILPALRLSYHHLPLDLRQCFAYCAVFPKDTKMIKENLI 427

Query: 125 KQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICELTMK 184
             W+A GF+ LS G   +ED+G E + EL  R F++   +  + +    +HD+I +L   
Sbjct: 428 TLWMAHGFL-LSKGNLELEDVGNEVWNELYLRSFFQ-EIEAKSGNTYFKIHDLIHDLATS 487

Query: 185 VAGKKFYVRGNLNNDYVVS-EQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYAN 244
           +        GN+    V   + T  I F  V+ S+   L K                   
Sbjct: 488 LFSASASC-GNIREINVKDYKHTVSIGFAAVVSSYSPSLLK------------------- 547

Query: 245 KNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLPNSI 304
                          F  LRVL+L  S + ++P+SI  L  LRYLDLS N     LP  +
Sbjct: 548 --------------KFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCN-NFRSLPERL 607

Query: 305 TRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSRRRIRHMPKGMEKLTCLQRITLFE 364
            +LQNLQTL + +C  L  LP+ TS L +LR L      +   P  +  LTCL+ +  F 
Sbjct: 608 CKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLTCLKTLGFFI 667

Query: 365 LDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEWKLSQ 424
           +  K+  +  EL   N   + S  IT LE++++     +  NL    +L+ L++ W    
Sbjct: 668 VGSKKGYQLGELKNLNLCGSIS--ITHLERVKNDTD--AEANLSAKANLQSLSMSWDNDG 727

Query: 425 EECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLL-MLTEITIANCHRLQHL 484
                + + +++E L+PHPN++ L I  + G   P+W++ S+L  +  + I +C     L
Sbjct: 728 PNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCL 746

BLAST of Lag0002750 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 8.8e-55
Identity = 155/481 (32.22%), Postives = 244/481 (50.73%), Query Frame = 0

Query: 5   FTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKT 64
           + L  L + D W LF + AF   +E  NPNL+ +GKEI+ K GGVPL  + +GG+L  K 
Sbjct: 307 YELSNLSQEDCWLLFMQRAF-GHQEEINPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKR 366

Query: 65  SEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINELI 124
            E  W   +++ +  + Q+ + +   L LSY+ LP  LKQCFAY ++FPK  K+E  +LI
Sbjct: 367 EERAWEHVRDSPIWNLPQDESSILPALRLSYHQLPLDLKQCFAYCAVFPKDAKMEKEKLI 426

Query: 125 KQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICELTMK 184
             W+A GF+ LS G   +ED+G E +KEL  R F++   +  +      MHD+I +L   
Sbjct: 427 SLWMAHGFL-LSKGNMELEDVGDEVWKELYLRSFFQ-EIEVKDGKTYFKMHDLIHDL--- 486

Query: 185 VAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANK 244
            A   F    + +N   + E  +H     + I + +V+                 ++   
Sbjct: 487 -ATSLFSANTSSSN---IREINKHSYTHMMSIGFAEVV-----------------FFYTL 546

Query: 245 NKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLPNSIT 304
             + K         F  LRVL+L  S   ++P+SI  L  LRYL+L  +  M  LP  + 
Sbjct: 547 PPLEK---------FISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGS-GMRSLPKQLC 606

Query: 305 RLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDF-SRRRIRHMPKGMEKLTCLQRITLFE 364
           +LQNLQTL L  C+ L  LP++TS L +LR L     + +  MP  +  LTCL+ +  F 
Sbjct: 607 KLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFV 666

Query: 365 LDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEWKLSQ 424
           +  K+  +  EL   N     S +I+ LE++++   +    NL    +L  L++ W    
Sbjct: 667 VGRKKGYQLGELGNLNLYG--SIKISHLERVKND-KDAKEANLSAKGNLHSLSMSWNNFG 726

Query: 425 EECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLM-LTEITIANCHRLQHL 484
                + + +++E L+PH N+  L I G+ G+ LP W++ S+L  +  I I+N      L
Sbjct: 727 PHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCL 747

BLAST of Lag0002750 vs. ExPASy TrEMBL
Match: A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.6e-168
Identity = 320/487 (65.71%), Postives = 375/487 (77.00%), Query Frame = 0

Query: 1    MTTLFTLKGLHENDSWPLFREVAFKDG-KEPANPNLIQLGKEILAKCGGVPLVIRHIGGL 60
            MTT  TLKGL E+ SW LFREVAF+     P NP L  +GKEI ++CGGVPL IRH+G  
Sbjct: 582  MTTSVTLKGLSEDRSWSLFREVAFEGNLPNPINPKLELMGKEISSRCGGVPLAIRHMGRF 641

Query: 61   LYSKTSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIE 120
            L SK  EEEWMS  +++LL+VTQ+NND+ SILELSYN LP HLKQCFAYSSLFPK +K++
Sbjct: 642  LCSKRCEEEWMSSLSDKLLEVTQQNNDVASILELSYNDLPLHLKQCFAYSSLFPKVHKLK 701

Query: 121  INELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVIC 180
            +NELI+QW+AQGFI+ S G KSME IGK YF ELC RFFYENSSDE NFDD V+MHDV+ 
Sbjct: 702  VNELIRQWVAQGFIESSKGEKSMETIGKNYFDELCWRFFYENSSDENNFDDYVYMHDVMY 761

Query: 181  ELTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHS 240
            EL  KVA KK YV G++NN+YVVSEQT H SFDY I SW+DVLSKL KAKGLRTF+ LHS
Sbjct: 762  ELARKVADKKLYVCGDINNNYVVSEQTLHFSFDYKIQSWQDVLSKLCKAKGLRTFMFLHS 821

Query: 241  YYANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELL 300
             Y  KN+VN+  LDELFSSFP LRVL L  S I+ +PNSI+KL+ LRYLDLSEN  M+ L
Sbjct: 822  PYEEKNEVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSEN-NMKSL 881

Query: 301  PNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR--RRIRHM-PKGMEKLTCL 360
            PNSIT LQ+LQTLKLT C  LKE+PRD + LVNLR LD SR   +I HM P+GMEKL+CL
Sbjct: 882  PNSITELQDLQTLKLTGCYALKEMPRDINKLVNLRHLD-SRCCSQITHMAPEGMEKLSCL 941

Query: 361  QRITLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLT 420
            Q I+LF  DC + +K  ELN+ NNL T   +ITGL+QLRS+P EVS VNLK  K LR L 
Sbjct: 942  QTISLFVFDCTKTNKLRELNELNNL-TGELKITGLQQLRSTPYEVSLVNLKDKKCLRSLK 1001

Query: 421  LEWKLSQEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLMLTEITIANC 480
            LEWK+   + E  +DER+M+GLEP+PNVE L I GY+GVGLP W+    L LTEI I +C
Sbjct: 1002 LEWKMGLYKHEDEADERVMKGLEPNPNVESLGIKGYNGVGLPKWMFNCHLKLTEIEIVSC 1061

Query: 481  HRLQHLP 484
            HRLQHLP
Sbjct: 1062 HRLQHLP 1065

BLAST of Lag0002750 vs. ExPASy TrEMBL
Match: A0A5A7UWP6 (Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00750 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 4.4e-166
Identity = 317/488 (64.96%), Postives = 374/488 (76.64%), Query Frame = 0

Query: 1   MTTLFTLKGLHENDSWPLFREVAFKDG-KEPANPNLIQLGKEILAKCGGVPLVIRHIGGL 60
           MTT  TLKGL E+ SW LFREVAF++   +P NP L  +GKEI + CGGVPL IRH+G  
Sbjct: 328 MTTSVTLKGLSEDSSWSLFREVAFEENLPKPINPKLELMGKEISSICGGVPLAIRHMGRF 387

Query: 61  LYSKTSEEEWMSFKN-NELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKI 120
           L SK  EEEWMS  +  + L+V Q+NND+ SILELSYN LP HLKQCFAYSSLFPKG+K+
Sbjct: 388 LCSKRCEEEWMSSLSWKKHLEVAQQNNDVASILELSYNDLPLHLKQCFAYSSLFPKGHKL 447

Query: 121 EINELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVI 180
           ++NELI+QW+AQGFI+ S G KSME IGK YF ELC RFFYENSSDE NFDD V+MHDV+
Sbjct: 448 KVNELIRQWVAQGFIESSKGEKSMETIGKNYFDELCWRFFYENSSDENNFDDYVYMHDVM 507

Query: 181 CELTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLH 240
            EL  KVA KK +V G++NN+YVVSEQT H S DY I SW+DVLSKL KAKGLRTF+ LH
Sbjct: 508 YELARKVADKKLFVCGDINNNYVVSEQTLHFSLDYKIQSWQDVLSKLCKAKGLRTFMFLH 567

Query: 241 SYYANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMEL 300
           S Y  KN+VN+  LDELFSSFP LRVL L  S I+ +PNSI+KL+ LRYLDLSEN  M+ 
Sbjct: 568 SPYEEKNEVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSEN-NMKS 627

Query: 301 LPNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR--RRIRHM-PKGMEKLTC 360
           LPNSIT LQ+LQTLKLT C  LKE+PRD + LVNLR LD SR   +I HM P+GMEKL+C
Sbjct: 628 LPNSITELQDLQTLKLTGCYALKEMPRDINKLVNLRHLD-SRCCSQITHMAPEGMEKLSC 687

Query: 361 LQRITLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDL 420
           LQ I+LF  DC + +K  ELN+ NNL T   +ITGL+QLRS+P EVS VNLKG K LR L
Sbjct: 688 LQTISLFVFDCTKTNKLRELNELNNL-TGELKITGLQQLRSTPYEVSLVNLKGKKCLRSL 747

Query: 421 TLEWKLSQEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLMLTEITIAN 480
            LEWK+   + E  +DER+M+GLEP+PNVE L I GY+GVGLP W+    L LTEI + +
Sbjct: 748 KLEWKMGLYKHEDEADERVMKGLEPNPNVESLGIKGYNGVGLPKWMFNCHLKLTEIEMVS 807

Query: 481 CHRLQHLP 484
           CHRLQHLP
Sbjct: 808 CHRLQHLP 812

BLAST of Lag0002750 vs. ExPASy TrEMBL
Match: A0A6J1D168 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111016078 PE=4 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.4e-164
Identity = 321/495 (64.85%), Postives = 377/495 (76.16%), Query Frame = 0

Query: 1   MTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLL 60
           MT+LFTLKGL E++SW LFR+VAFK+ K+PAN NLI+LGKEILAKCGGVPLVIRHIG LL
Sbjct: 328 MTSLFTLKGLQESNSWSLFRKVAFKEAKDPANSNLIKLGKEILAKCGGVPLVIRHIGRLL 387

Query: 61  YSKTSEEEWMSFKNNELLKV---TQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNK 120
           YSKTSEEEWMSFKNNELLKV   T +N+ +T IL LSYNHLP +LKQCFAY SLFPKG +
Sbjct: 388 YSKTSEEEWMSFKNNELLKVIQQTDKNDGLTPILNLSYNHLPPNLKQCFAYLSLFPKGTR 447

Query: 121 IEINELIKQWIAQGFIKLSNG-GKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHD 180
           +EI +LI+QW+A GFI+ SNG   SME++GK YFKELC RFF+ENSSDE NFDD V MHD
Sbjct: 448 LEIKDLIRQWMAHGFIESSNGTANSMEEVGKSYFKELCWRFFFENSSDECNFDDDVDMHD 507

Query: 181 VICELTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLL 240
           V+ E+ M VAG+K YV GNLN D+VVSE+TRHI FD  I  W+DVL KL+KAKGLRTFL 
Sbjct: 508 VMREVAMNVAGEK-YVHGNLNCDFVVSEKTRHILFDSKIKLWQDVLLKLQKAKGLRTFLF 567

Query: 241 LHSYYANKNKVN---KTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSEN 300
           L SY+  KN++    + +L+EL SSFP LRVL L  S I+ VPNSI+ LR LRYL+LS N
Sbjct: 568 L-SYFPFKNRLEDNIEAVLEELCSSFPRLRVLGLNHSKIHVVPNSIKNLRHLRYLNLSRN 627

Query: 301 WRMELLPNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSRR--RIRHMPKGME 360
           + ME LP SIT+LQNLQ+L L  C  L ELPRDTSNLVNLR L          HM +GM 
Sbjct: 628 Y-MESLPTSITKLQNLQSLDLKDCYKLNELPRDTSNLVNLRHLVLPDHLDTPLHMLEGMR 687

Query: 361 KLTCLQRITLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKD 420
           KL+CL+ +  F LDCKR +  SELN  N  + +  +I GLEQLR +PS+V  VNLK  + 
Sbjct: 688 KLSCLRTLGYFVLDCKRSNMQSELNVPNYFNGR-LKIVGLEQLRRTPSKVGLVNLKDIQG 747

Query: 421 LRDLTLEWKLS---QEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSCSLLML 480
           LRDL L WKLS    +E EG  DER+MEGL+P  NVE L I+GYSGVGLPNWVS SL  L
Sbjct: 748 LRDLKLVWKLSGRDDDEGEGKGDERVMEGLQPQSNVESLRIEGYSGVGLPNWVSTSLSNL 807

Query: 481 TEITIANCHRLQHLP 484
           T+I I NCHR+QHLP
Sbjct: 808 TKIAICNCHRVQHLP 818

BLAST of Lag0002750 vs. ExPASy TrEMBL
Match: A0A5A7US10 (Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00810 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 7.8e-163
Identity = 320/489 (65.44%), Postives = 366/489 (74.85%), Query Frame = 0

Query: 1   MTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLL 60
           MT   TL  L E+ SW LF++VAFK G++     + QLGKEIL KCGGVPLVIRHIG LL
Sbjct: 336 MTKSITLDVLDEDSSWSLFKKVAFKQGQDSKYLEVEQLGKEILKKCGGVPLVIRHIGRLL 395

Query: 61  YSKTSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEI 120
            SKTS+EEWMSF +NE L+VTQ++N MTSILELSYN+LP HLKQCFAYSSLFPKG KI+I
Sbjct: 396 SSKTSKEEWMSFNDNESLEVTQQDNHMTSILELSYNNLPPHLKQCFAYSSLFPKGYKIKI 455

Query: 121 NELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICE 180
           NELI QWIAQ FI+ S+G KS+++IGK+YF ELCSRFFYEN S + NFDD V MHDV+ E
Sbjct: 456 NELIGQWIAQDFIESSSGRKSLDNIGKDYFDELCSRFFYENLSGDNNFDDYVRMHDVMRE 515

Query: 181 LTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSY 240
           L  KVAG K+Y RG+ NN YVVSEQTRHISFDY I  WRDVLSKL  AKGL+TFL+LH  
Sbjct: 516 LAKKVAGNKYYARGDSNNSYVVSEQTRHISFDYKIELWRDVLSKLLTAKGLKTFLILHHL 575

Query: 241 YANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLP 300
           Y  KN V++  LD+LFSSFP LRVL L+ S I  VP+SI KL  LRYLDLSEN  +E LP
Sbjct: 576 YEKKNTVDRETLDKLFSSFPRLRVLGLHDSKISTVPDSIEKLTDLRYLDLSEN-DIESLP 635

Query: 301 NSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR-RRIRHMPKGMEKLTCLQRI 360
           NSIT+LQNLQTLKLT C  LKELPR+T  LVNLR L F    +I HMP+GMEKLT LQ I
Sbjct: 636 NSITKLQNLQTLKLTECHKLKELPRNTKKLVNLRHLAFDHCIQITHMPEGMEKLTSLQTI 695

Query: 361 TLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSP---SEVSSVNLKGGKDLRDLT 420
           TLF  DCK+ +K SELN+  N S    +ITGLEQLRS      EV  VNLK G   R L 
Sbjct: 696 TLFVFDCKKSNKLSELNEL-NYSKAELKITGLEQLRSIDFILPEVGLVNLKHG--WRSLK 755

Query: 421 LEWKLS-QEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSC-SLLMLTEITIA 480
           LEW+LS  +E EG  D++IME L PHP +E LSI GY GVGLP WVS      LT+I I 
Sbjct: 756 LEWELSGGDEYEGEGDKKIMECLIPHPYLESLSIKGYCGVGLPEWVSAWPFWKLTQIEIV 815

Query: 481 NCHRLQHLP 484
           NCHRLQ+LP
Sbjct: 816 NCHRLQYLP 820

BLAST of Lag0002750 vs. ExPASy TrEMBL
Match: A0A1S3CFN9 (LOW QUALITY PROTEIN: putative disease resistance protein RGA3 OS=Cucumis melo OX=3656 GN=LOC103499942 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 1.1e-161
Identity = 319/489 (65.24%), Postives = 364/489 (74.44%), Query Frame = 0

Query: 1   MTTLFTLKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLL 60
           MT   TL  L E+ SW LF++VAFK G++     + QLGKEIL KCGGVPLVIRHIG LL
Sbjct: 336 MTKSITLDVLDEDSSWSLFKKVAFKQGQDSKYLEVEQLGKEILKKCGGVPLVIRHIGRLL 395

Query: 61  YSKTSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEI 120
            SKTS+EEWMSF +NE L+VTQ++N MTSILELSYN+LP HLKQCFAYSSLFPKG KI+I
Sbjct: 396 SSKTSKEEWMSFNDNESLEVTQQDNHMTSILELSYNNLPPHLKQCFAYSSLFPKGYKIKI 455

Query: 121 NELIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICE 180
           NELI QWIAQ FI+ S+G KS+++IGK+YF ELCSRFFYEN S + NFDD V MHDV+ E
Sbjct: 456 NELIGQWIAQDFIESSSGRKSLDNIGKDYFDELCSRFFYENLSGDNNFDDYVRMHDVMRE 515

Query: 181 LTMKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSY 240
           L  KVAG K+Y RG+ NN YVVSEQTRHISFDY I  WRDVLSKL  AKGL+TFL+LH  
Sbjct: 516 LAKKVAGNKYYARGDSNNSYVVSEQTRHISFDYKIELWRDVLSKLLTAKGLKTFLILHHL 575

Query: 241 YANKNKVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLP 300
           Y  KN V++  LD+LFSSFP LRVL L+ S I  VP+SI KL  LRYLDLSEN  +E LP
Sbjct: 576 YEKKNTVDRETLDKLFSSFPRLRVLGLHDSKISTVPDSIEKLTDLRYLDLSEN-DIESLP 635

Query: 301 NSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR-RRIRHMPKGMEKLTCLQRI 360
           NSIT+LQNLQTLKLT C  LKELPR+T  LVNLR L F    +I HMP+GMEKLT LQ I
Sbjct: 636 NSITKLQNLQTLKLTECHKLKELPRNTKKLVNLRHLAFDHCIQITHMPEGMEKLTSLQTI 695

Query: 361 TLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSP---SEVSSVNLKGGKDLRDLT 420
           TLF  DCK+ +K SELN+  N S    +ITGLEQLRS      EV  VNLK G   R L 
Sbjct: 696 TLFVFDCKKSNKLSELNEL-NYSKAELKITGLEQLRSIDFILPEVGLVNLKHG--WRSLK 755

Query: 421 LEWKLS-QEECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVSC-SLLMLTEITIA 480
           LEW+LS  +E EG  D+ IM  L PHP +E LSI GY GVGLP WVS      LT+I I 
Sbjct: 756 LEWELSGGDEYEGEGDKTIMVCLIPHPYLESLSIKGYCGVGLPEWVSAWPFWKLTQIEIV 815

Query: 481 NCHRLQHLP 484
           NCHRLQ+LP
Sbjct: 816 NCHRLQYLP 820

BLAST of Lag0002750 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 242.3 bits (617), Expect = 8.2e-64
Identity = 155/487 (31.83%), Postives = 259/487 (53.18%), Query Frame = 0

Query: 7   LKGLHENDSWPLFREVAFKDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSE 66
           L+ L + D W LF +  F + +   N  +  L + I+ KC G+PL ++ +GG+L  +   
Sbjct: 331 LQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKV 390

Query: 67  EEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINELIKQ 126
            EW    ++ +  +  + +++  +L +SY +LP HLK+CFAY S+FPKG+  E ++++  
Sbjct: 391 IEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLL 450

Query: 127 WIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICELTMKVA 186
           W+A+GF++ +   K++E++G EYF EL SR   + +   Y       MHD I EL    +
Sbjct: 451 WMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRY------IMHDFINELAQFAS 510

Query: 187 GKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANKN- 246
           G +F  +        VSE+TR++S+     +       LR+ K LRTFL L    ++++ 
Sbjct: 511 G-EFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSC 570

Query: 247 KVNKTILDELFSSFPHLRVLSLYASYIYEV-PNSIRKLRQLRYLDLSENWRMELLPNSIT 306
            +++ + ++L  +   LRVLSL    I  + P+  + +   R+LDLS    +E LP S+ 
Sbjct: 571 CLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLC 630

Query: 307 RLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSRRRIRHMPKGMEKLTCLQRITLFEL 366
            + NLQTL L+ CS LKELP D SNL+NLR LD    ++R MP+   +L  LQ +T F +
Sbjct: 631 YMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFV 690

Query: 367 DCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEWKLSQE 426
                S+ SEL   ++L  +  +I  L+++    ++ +  NL   K LR++   W+    
Sbjct: 691 SASDGSRISELGGLHDLHGK-LKIVELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSS 750

Query: 427 ECEG-------ASDERIMEGLEPHPNVEILSIDGYSGVGLPNWVS-CSLLMLTEITIANC 484
             E         ++  + E L PH ++E L+I+ Y G   P+W+S  S   +  I +  C
Sbjct: 751 SSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLREC 807

BLAST of Lag0002750 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.4e-52
Identity = 145/490 (29.59%), Postives = 244/490 (49.80%), Query Frame = 0

Query: 4   LFTLKGLHENDSWPLFREVAFKD-GKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYS 63
           ++ +K +   + W L    AF +      N  L  +GK I  +C G+PL  R I   L S
Sbjct: 323 IYQMKLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRS 382

Query: 64  KTSEEEWMSFKNNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINE 123
           K + ++W +   N     +   N +  +L+LSY+ LP  LK+CFA  S+FPKG+  +  E
Sbjct: 383 KPNPDDWYAVSKN----FSSYTNSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREE 442

Query: 124 LIKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYNFDDCVHMHDVICELT 183
           L+  W+A   +      + +EDIG +Y  +L ++ F++    +      V MHD++ +L 
Sbjct: 443 LVLLWMAIDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRL--DITMTSFV-MHDLMNDLA 502

Query: 184 MKVAGKKFYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYA 243
             V+G  F  R   +N   +   TRH SF             +  A+ LRT L  +S  +
Sbjct: 503 KAVSG-DFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTS 562

Query: 244 NKN-KVNKTILDELFSSFPHLRVLSLYASYIYEVPNSIRKLRQLRYLDLSENWRMELLPN 303
            ++ ++ + +L+ L ++   LR+LSL    I  +P S++ L+ LRYLDLS   +++ LP 
Sbjct: 563 LESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSST-KIKELPE 622

Query: 304 SITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSRRRIRHMPKGMEKLTCLQRITL 363
            +  L NLQTL L++C  L  LP+  + L+NLR LD     +  MP G++KL  LQ+++ 
Sbjct: 623 FVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRSLQKLSN 682

Query: 364 FELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDLRDLTLEWKL 423
           F +     +   EL + ++L   + RI+ L+ + +  SE     LK    L  L L+W +
Sbjct: 683 FVIGRLSGAGLHELKELSHL-RGTLRISELQNV-AFASEAKDAGLKRKPFLDGLILKWTV 742

Query: 424 SQE-------ECEGASDERIMEGLEPHPNVEILSIDGYSGVGLPNWV-SCSLLMLTEITI 483
                            + ++  LEPHP+++   I+ Y G   P W+   S   +T +T+
Sbjct: 743 KGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGITSVTL 801

BLAST of Lag0002750 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 133.3 bits (334), Expect = 5.3e-31
Identity = 138/472 (29.24%), Postives = 214/472 (45.34%), Query Frame = 0

Query: 10  LHENDSWPLFREVAF--KDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTS-E 69
           L  ++SW LF  VAF   DG     P L  +GKEI+ KC G+PL I+ +GGLL  K    
Sbjct: 318 LSPDNSWLLFCNVAFAANDG-TCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVY 377

Query: 70  EEWMSFK---NNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINEL 129
            EW        +EL   T E +++ S L+LSY+ LP+HLK C    SL+P+   I   +L
Sbjct: 378 HEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQL 437

Query: 130 IKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYN--FDDCVHMHDVICEL 189
           +  WI +GF+   N G+S  + G++ F  L +R   E     Y+     C  +HD++ +L
Sbjct: 438 VHGWIGEGFVMWRN-GRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITC-KIHDMVRDL 497

Query: 190 TMKVAGKKFYVRGNLNNDYVVSEQTRHI----SFDYVIISWRDVLSKLRKAKGLRTFLLL 249
            + +A K  +      N        RH+    +FD   I    V  KLR        ++ 
Sbjct: 498 VIDIAKKDSFSNPEGLN-------CRHLGISGNFDEKQIK---VNHKLRG-------VVS 557

Query: 250 HSYYANKNKVNKTILDELFSSFPHLRVL----SLYASYIYEVPNSIRKLRQLRYLDLSEN 309
            +     NK+N   L + F+   +LRVL    S++ + + E+ + I  L+ L  L LS  
Sbjct: 558 TTKTGEVNKLNSD-LAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNT 617

Query: 310 WRMELLPNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR-RRIRHMPKGMEK 369
             +   P S+  L NLQ L  + C  LK+L         L  LD +    +   PKG+  
Sbjct: 618 HPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGS 677

Query: 370 LTCLQRITLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDL 429
           L  L+ +  F        KP+  N    LS +   +T L +L  S +    +  +    L
Sbjct: 678 LVKLEVLLGF--------KPARSNNGCKLS-EVKNLTNLRKLGLSLTRGDQIEEEELDSL 737

Query: 430 RDLTLEWKLSQEECEGASDERI--MEGLEPHPNVEILSIDGYSGVGLPNWVS 463
            +L+    +S    +   D+ I  ++ L P   +  LS+  Y G   P+W+S
Sbjct: 738 INLSKLMSISINCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSSPSWLS 759

BLAST of Lag0002750 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 133.3 bits (334), Expect = 5.3e-31
Identity = 138/472 (29.24%), Postives = 214/472 (45.34%), Query Frame = 0

Query: 10  LHENDSWPLFREVAF--KDGKEPANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTS-E 69
           L  ++SW LF  VAF   DG     P L  +GKEI+ KC G+PL I+ +GGLL  K    
Sbjct: 318 LSPDNSWLLFCNVAFAANDG-TCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVY 377

Query: 70  EEWMSFK---NNELLKVTQENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKIEINEL 129
            EW        +EL   T E +++ S L+LSY+ LP+HLK C    SL+P+   I   +L
Sbjct: 378 HEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQL 437

Query: 130 IKQWIAQGFIKLSNGGKSMEDIGKEYFKELCSRFFYENSSDEYN--FDDCVHMHDVICEL 189
           +  WI +GF+   N G+S  + G++ F  L +R   E     Y+     C  +HD++ +L
Sbjct: 438 VHGWIGEGFVMWRN-GRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITC-KIHDMVRDL 497

Query: 190 TMKVAGKKFYVRGNLNNDYVVSEQTRHI----SFDYVIISWRDVLSKLRKAKGLRTFLLL 249
            + +A K  +      N        RH+    +FD   I    V  KLR        ++ 
Sbjct: 498 VIDIAKKDSFSNPEGLN-------CRHLGISGNFDEKQIK---VNHKLRG-------VVS 557

Query: 250 HSYYANKNKVNKTILDELFSSFPHLRVL----SLYASYIYEVPNSIRKLRQLRYLDLSEN 309
            +     NK+N   L + F+   +LRVL    S++ + + E+ + I  L+ L  L LS  
Sbjct: 558 TTKTGEVNKLNSD-LAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNT 617

Query: 310 WRMELLPNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRQLDFSR-RRIRHMPKGMEK 369
             +   P S+  L NLQ L  + C  LK+L         L  LD +    +   PKG+  
Sbjct: 618 HPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGS 677

Query: 370 LTCLQRITLFELDCKRLSKPSELNKFNNLSTQSWRITGLEQLRSSPSEVSSVNLKGGKDL 429
           L  L+ +  F        KP+  N    LS +   +T L +L  S +    +  +    L
Sbjct: 678 LVKLEVLLGF--------KPARSNNGCKLS-EVKNLTNLRKLGLSLTRGDQIEEEELDSL 737

Query: 430 RDLTLEWKLSQEECEGASDERI--MEGLEPHPNVEILSIDGYSGVGLPNWVS 463
            +L+    +S    +   D+ I  ++ L P   +  LS+  Y G   P+W+S
Sbjct: 738 INLSKLMSISINCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSSPSWLS 759

BLAST of Lag0002750 vs. TAIR 10
Match: AT1G58807.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 124.0 bits (310), Expect = 3.2e-28
Identity = 104/368 (28.26%), Postives = 170/368 (46.20%), Query Frame = 0

Query: 10  LHENDSWPLFREVA--FKDGKE-PANPNLIQLGKEILAKCGGVPLVIRHIGGLLYSKTSE 69
           L   DSW LF+ +A   KD  E   +    +LGK ++  CGG+PL IR +GG+L  K + 
Sbjct: 321 LTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGLPLAIRVLGGMLAEKYTS 380

Query: 70  EEWMSFKNNELLKVT--------QENNDMTSILELSYNHLPTHLKQCFAYSSLFPKGNKI 129
            +W     N    +           NN   ++L LS+  LP++LK CF Y + FP+  +I
Sbjct: 381 HDWRRLSENIGSHLVGGRTNFNDDNNNTCNNVLSLSFEELPSYLKHCFLYLAHFPEDYEI 440

Query: 130 EINELIKQWIAQGFIKLSN-GGKSMEDIGKEYFKELCSR--FFYENSSDEYNFDDCVHMH 189
           ++  L   W A+G  +  +  G+++ D+G  Y +EL  R     E       F+ C H+H
Sbjct: 441 KVENLSYYWAAEGIFQPRHYDGETIRDVGDVYIEELVRRNMVISERDVKTSRFETC-HLH 500

Query: 190 DVICELTMKVAGKKFYVR--------GNLNNDYVVSEQ-------TRHISFD-------- 249
           D++ E+ +  A ++ +++         NL +  V S +       T H+  D        
Sbjct: 501 DMMREVCLLKAKEENFLQITSSRPSTANLQST-VTSRRFVYQYPTTLHVEKDINNPKLRA 560

Query: 250 YVII---SWRDVLSKLRKAKGLRTFLLLHSYYANKNKVNKTILDELFSSFPHLRVLSLYA 309
            V++   SW    S   + + LR   L+      + K+    L        HLR LSL  
Sbjct: 561 LVVVTLGSWNLAGSSFTRLELLRVLDLI------EVKIKGGKLASCIGKLIHLRYLSLEY 620

Query: 310 SYIYEVPNSIRKLRQLRYLDLSENWRMELLPNSITRLQNLQTLKLTSCSGLKELPRDTSN 338
           + +  +P S+  L+ L YL+L+   R   +PN +  +Q L+ L L S  G ++   + SN
Sbjct: 621 AEVTHIPYSLGNLKLLIYLNLASFGRSTFVPNVLMGMQELRYLALPSDMG-RKTKLELSN 679

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_031745107.16.1e-17867.56putative disease resistance protein RGA4 [Cucumis sativus][more]
KAE8646395.16.1e-17867.56hypothetical protein Csa_016171 [Cucumis sativus][more]
XP_038897566.13.9e-16967.01putative disease resistance protein RGA4 [Benincasa hispida][more]
XP_008461332.23.3e-16865.71PREDICTED: disease resistance protein RGA2 [Cucumis melo][more]
KAA0058606.19.1e-16664.96disease resistance protein RGA2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9LRR41.2e-6231.83Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Q7XA401.5e-6233.40Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA429.7e-6232.71Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA391.2e-5933.26Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XBQ98.8e-5532.22Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CEH41.6e-16865.71disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=... [more]
A0A5A7UWP64.4e-16664.96Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A6J1D1681.4e-16464.85putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A5A7US107.8e-16365.44Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CFN91.1e-16165.24LOW QUALITY PROTEIN: putative disease resistance protein RGA3 OS=Cucumis melo OX... [more]
Match NameE-valueIdentityDescription
AT3G14470.18.2e-6431.83NB-ARC domain-containing disease resistance protein [more]
AT3G14460.11.4e-5229.59LRR and NB-ARC domains-containing disease resistance protein [more]
AT3G50950.25.3e-3129.24HOPZ-ACTIVATED RESISTANCE 1 [more]
AT3G50950.15.3e-3129.24HOPZ-ACTIVATED RESISTANCE 1 [more]
AT1G58807.13.2e-2828.26Disease resistance protein (CC-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 95..184
e-value: 1.9E-12
score: 49.1
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 9..94
e-value: 1.9E-14
score: 55.0
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 7..96
e-value: 2.7E-11
score: 43.2
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 218..483
e-value: 5.9E-35
score: 122.2
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 10..479
NoneNo IPR availablePANTHERPTHR23155:SF1055OS05G0414200 PROTEINcoord: 10..479
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 174..483
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 4..108

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002750.1Lag0002750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding