Homology
BLAST of Lag0002715 vs. NCBI nr
Match:
XP_022154678.1 (putative disease resistance protein RGA4 [Momordica charantia])
HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 630/1171 (53.80%), Postives = 784/1171 (66.95%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MADSIL+N+ +I KLG +E+ +L G++ ELDKL+ T+SAI VLLDAEER ++
Sbjct: 1 MADSILFNLAGTLINKLGXYVHQELGLLLGVNGELDKLKGTVSAIXKVLLDAEERSSKSH 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLK----SHLKI 120
A+ DW+SKLK VFYEIDDL+DEF+YE +RRQVM R+ ++VR FFSK SH K+
Sbjct: 61 ALEDWLSKLKGVFYEIDDLIDEFNYEALRRQVMTRNRRKTKQVRIFFSKSNQIAFSH-KM 120
Query: 121 DKKIKQIRDTLEAINHDKTQFSFSERVID-TRPDELRARRDIGSFIPEEEVIGRNDDKEN 180
KIK+IR+ L+ IN DKTQF SE VI+ + D LR RD G +IPEEEVIGRN +K+
Sbjct: 121 GGKIKEIREKLKVINDDKTQFKLSECVINKSDVDILRNTRDPGDYIPEEEVIGRNYEKKE 180
Query: 181 VIHRLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVK 240
+I L N+N +EDIA+++IVGMGGLGKTT+AQSVY+ +TKHF+L+LWVC+SEEF+VK
Sbjct: 181 IIDLLLNSN-AREDIAVVAIVGMGGLGKTTIAQSVYSDKMMTKHFELRLWVCISEEFEVK 240
Query: 241 IIIQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLM 300
+IIQ IIE AT + +SF QM LQ ELR+ +DGKKYLLVMDDVWNE+HEKW LKRLLM
Sbjct: 241 VIIQNIIEYAT-RRKESFPQMALLQTELRKLVDGKKYLLVMDDVWNEDHEKWNTLKRLLM 300
Query: 301 GGAKGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMT-GLEGQEKELENSDL 360
GGAKGSRILITTR+ +VA TFD+T YSLE LN+ NSWLLF+KM G EG EK+ ENS L
Sbjct: 301 GGAKGSRILITTRTHQVANTFDSTSXYSLEELNDKNSWLLFEKMAFGGEGAEKDPENSKL 360
Query: 361 IEIGKEIVAKLKAM---------------------------------------------- 420
+EIGKEIV KLK +
Sbjct: 361 VEIGKEIVTKLKGIPLAIRTVGRLLYYKKSTEFWSSFKDKKLYQIIEQGSLKDMLSILML 420
Query: 421 ------------LFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDYFLELL 480
YCA+FPKDYEIQK+E+I+QW AQ FI PNDK + IDIGE+YF+ELL
Sbjct: 421 SYNHLPYNLKQCFLYCAMFPKDYEIQKNEVIMQWIAQSFIHPNDKYKFIDIGEEYFMELL 480
Query: 481 SKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLK-NGIDKRTHHFSSTWMPR 540
S+SFFQ+VTK++LG+I CKMHDLMHDLACSI +NECVH + + KRTHH S M
Sbjct: 481 SRSFFQDVTKNDLGDIITCKMHDLMHDLACSIVENECVHDVNARTLSKRTHHVSFDLMSW 540
Query: 541 KDSEKLLQRLSKARNLRT-FFMLNYM---DGWKLECDKFYHDHLRLRTLRLRLSPMD-QS 600
++ E+L + LS+A+NLRT FF N + + + +++ LRLRTL L+L D Q+
Sbjct: 541 EELERLPETLSEAKNLRTCFFHYNQRRTDNNFNEIFESVFYNLLRLRTLDLQLLSFDKQN 600
Query: 601 AKFLGSIIGTFKHLRYLSITSF-NLKNLPNCITELYNLETFILQYSSRLETLPSDINNLI 660
KFL I KHLRYL I + + N IT LYNLE FIL+ +RL+ LP DI N
Sbjct: 601 VKFL-KCISKLKHLRYLEIQMYTKTEFFMNHITGLYNLEVFILR-CTRLKELPRDIKNWN 660
Query: 661 NLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLSWNKS 720
L+H+ L +N+ ++FLP+S EL+ LETL+LH C KLKEL DV+NWINLR+LDLS +
Sbjct: 661 ELKHVYLYKNEIIEFLPNSFAELNNLETLVLHQCTKLKELLGDVRNWINLRYLDLS-SSG 720
Query: 721 IEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGLGQLT 780
+EFLPDSITQL NLE LLL +CS L+ELP DTK LINLKH+D+ G ALTHMPKGLG+LT
Sbjct: 721 VEFLPDSITQLKNLETLLLRFCSKLQELPRDTKNLINLKHVDLYGCWALTHMPKGLGELT 780
Query: 781 NLQTLNLFVL-GDNNGGGLNELNELNKLKGRLSIEKLQFCNVIDLQMKDKLLQLKSCLQV 840
+LQ LN FVL DNNGGGL+ELNELNKL+G L+I +L FC DL K L LKS LQ
Sbjct: 781 DLQILNFFVLEKDNNGGGLSELNELNKLRGSLTINRLNFCTTSDLPTNAKFLLLKSSLQE 840
Query: 841 LWLQW---SRSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISGYKGVKLCNWLSSNF 900
L L+W + S NEPTIG+LD++ E VL+CL PHSNLR + I Y+GVKLCNW+SSNF
Sbjct: 841 LVLEWEWDNDSVNEPTIGDLDDD--CESVLECLHPHSNLRNLCIDKYRGVKLCNWVSSNF 900
Query: 901 LGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEYIGNDDSVSSSTFFPSLEELK 960
LGCLV+I+LS C RL+HLPQFDQFP+LK L +L IEYI NDD SST FPSLEEL
Sbjct: 901 LGCLVTIKLSGCKRLQHLPQFDQFPNLKRLSLRELSGIEYIFNDDYRCSSTLFPSLEELS 960
Query: 961 IVRMPKLKRWWKGTTSTEST-------PIKFPCLSQLEIRGCPLRILMSWHAPLKSLTIS 1020
I MP LKRWWK ++++T PI FPCLS+LEI GCPL ++ HAP KSL ++
Sbjct: 961 IFCMPNLKRWWKKGQTSKATSSQNVLFPIIFPCLSKLEIWGCPLLASITCHAPPKSLEMN 1020
Query: 1021 DS-----EVNEFDVVPWQNFENLSSLILFNFSKLEYLPKCLQHVTTLQNLRLWDCENLKS 1037
D + +E DVV W+ FE+LS L L + + +L LQHVTTLQ L L C+NL +
Sbjct: 1021 DPIEFHFDPDESDVVSWKCFESLSYLRLTHIDEWGFLSNWLQHVTTLQELTLEYCQNLTN 1080
BLAST of Lag0002715 vs. NCBI nr
Match:
XP_016902648.1 (PREDICTED: putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo])
HSP 1 Score: 1071.6 bits (2770), Expect = 4.0e-309
Identity = 606/1117 (54.25%), Postives = 756/1117 (67.68%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MA++ILYN A+II LGS A++EI LW ++ EL KL+ +LSAIQAVL DAEE+ N
Sbjct: 1 MAEAILYNFTADIIFTLGSSALQEIGSLWRVNSELHKLKHSLSAIQAVLHDAEEQQSKNN 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLKSHLKIDKKI 120
+ DW+ KL+DV YEIDDL+DE SY+T+RRQV+ + ++VR FSK KS+ KI KI
Sbjct: 61 QVKDWVLKLQDVLYEIDDLIDESSYQTLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHKI 120
Query: 121 KQIRDTLEAINHDKTQFSFSERVIDTRPDE--LRARRDIGSFIPEEEVIGRNDDKENVIH 180
K+IR L+AIN DK QFSF + VI+ R D+ LR RR+ SFI E+EVIGRNDDKE VI
Sbjct: 121 KEIRQRLQAINEDKNQFSFRKHVIERRDDDEGLRKRRETHSFILEDEVIGRNDDKEAVID 180
Query: 181 RLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKIII 240
L N+N T+EDIA++SIVGMGGLGKT LA+S+YNH I F LKLWVCVS+ FD++II+
Sbjct: 181 LLLNSN-TKEDIAIVSIVGMGGLGKTALARSIYNHKRIMTRFSLKLWVCVSDAFDLEIIV 240
Query: 241 QKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMGGA 300
QKIIESATGK +LQ+DSLQ ELR+ IDG KYL VMDDVWNE E+W+ LK LLM GA
Sbjct: 241 QKIIESATGKK-SGYLQIDSLQCELRKHIDGNKYLFVMDDVWNEKKEEWLHLKELLMAGA 300
Query: 301 KGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLE----GQEKELE--NS 360
KGSRILITTRSE+VA+TFD+TF++ L+ L+ NSWLLF+KMTGLE QE E++ N
Sbjct: 301 KGSRILITTRSEQVAKTFDSTFVHPLQILDASNSWLLFQKMTGLEELSNNQEAEIDQTNL 360
Query: 361 DLIEIGKEIVAKLKAM-------------------------------------------- 420
+LI+IGKEI+++LK +
Sbjct: 361 NLIQIGKEILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVLRQGQDALKEMKY 420
Query: 421 ----------------LFYCALFPKDYEIQKDELILQWRAQGFIQPN-DKD-ELIDIGED 480
YCALFPKD+E+ K LIL WR+QG+IQPN +KD L+DIG+
Sbjct: 421 ILELSYKYLPANLKQCFLYCALFPKDFEVPKISLILLWRSQGYIQPNGNKDNNLVDIGDG 480
Query: 481 YFLELLSKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLK-NGIDKRTHHFS 540
YF+ELLS+SFFQ+V KD+ G+I ACKMHD+MHDLACS+T NECVHGLK N I+KRT HF
Sbjct: 481 YFMELLSRSFFQDVKKDDSGDIVACKMHDMMHDLACSLTNNECVHGLKGNIINKRTRHFV 540
Query: 541 STWMPRKD--SEKLLQRLSKARNLRTFFMLNYMD--GWKLECDKFYHDHLRLRTLRLRLS 600
+ KD ++L++ LSKA +LRT F D G + ++ + R RT+ L
Sbjct: 541 ---LEDKDYHEDQLMRPLSKATHLRTLFYRKEEDVIGSQWNFEETFRSIFRFRTVHLEGY 600
Query: 601 PMDQSAKFLGSIIGTFKHLRYLSITSFNLKNLPNCITELYNLETFILQYSSRLETLPSDI 660
+ AK L IG FKHLR+LSIT N+ LP+ IT+LY LETF Q S LE LP+D+
Sbjct: 601 DQNPKAKAL-EFIGKFKHLRFLSITYSNIPYLPDSITKLYYLETFFFQCSHNLENLPNDV 660
Query: 661 NNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLS 720
NLINL+HLDL N TL+FLPDSITEL KLE LIL C KLK LP+++KN NLR LDLS
Sbjct: 661 GNLINLKHLDLSHNSTLEFLPDSITELCKLEALILKGCEKLKRLPKNIKNCNNLRQLDLS 720
Query: 721 WNKSIEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGL 780
N+ IEFLPDSITQL NLE L+L++C NLKELPEDTK+LINLK L + ALTHMPKGL
Sbjct: 721 HNERIEFLPDSITQLINLETLILSWCINLKELPEDTKRLINLKQLYLEICTALTHMPKGL 780
Query: 781 GQLTNLQTLNLFVLGDNNGGGLNELNELNKLKGRLSIEKLQFC-NVIDLQMKDKLLQLKS 840
G+LTNLQTLN FVLG+N GG L EL+ L KL+G+LSIE L+FC N++D +MK K LQLKS
Sbjct: 781 GELTNLQTLNTFVLGNNKGGELKELDGLTKLRGKLSIEHLEFCTNIVDHKMKGKFLQLKS 840
Query: 841 CLQVLWLQWSRSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISGYKGVKLCNW-LSS 900
LQ L L W + TIG L+ + +YE VLDCLQPHSNL++I I GY GV LCNW +S
Sbjct: 841 GLQELKLHWEKH----TIGELE-DVIYESVLDCLQPHSNLQKIYIDGYGGVNLCNWVVSP 900
Query: 901 NFLGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEYI--GNDDSVSSSTFFPSL 960
FLGCLV++ L C RL HLP+FDQFP+LK+L+ DLP+IEYI NDDSVSSST FPSL
Sbjct: 901 KFLGCLVTMRLCNCERLRHLPKFDQFPNLKHLYLIDLPNIEYIIANNDDSVSSSTNFPSL 960
Query: 961 EELKIVRMPKLKRWWKGTTSTESTPIKFPCLSQLEIRGCPLRILMSWHAPLKSLTISDSE 1020
E L I+ MPKL W KGTTST+S+ K
Sbjct: 961 ENLIILNMPKLVSWCKGTTSTKSSITK--------------------------------- 1020
Query: 1021 VNEFDVVPWQNFENLSSLILFNFSKLEYLPKCLQHVTTLQNLRLWDCENLKSLPEWMDNL 1039
+ + P+ +L SL+L N L+ LP+ + ++T+L +L + C+ L LPE + +
Sbjct: 1021 -SSIIIFPYHYMISLQSLLLKNCQNLKSLPEWIGNLTSLTSLYISFCKKLTMLPEGIRHT 1072
BLAST of Lag0002715 vs. NCBI nr
Match:
XP_022154711.1 (LOW QUALITY PROTEIN: disease resistance protein RGA2-like [Momordica charantia])
HSP 1 Score: 1061.6 bits (2744), Expect = 4.3e-306
Identity = 610/1091 (55.91%), Postives = 752/1091 (68.93%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MADSIL+N+ +ITKLGS A++E+ +L G++DEL+KL+DT+SAI+AVLLDAEE+P ++
Sbjct: 1 MADSILFNLAGTLITKLGSYALQELGLLLGVNDELEKLKDTVSAIEAVLLDAEEQPSKSH 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLK----SHLKI 120
A+ DW+SKLKDVFYEIDDL+DEF YE +RRQVM R+ T ++VR FFSK SH K+
Sbjct: 61 ALKDWLSKLKDVFYEIDDLIDEFEYEALRRQVMTRNRTKTKQVRIFFSKSNQIAFSH-KM 120
Query: 121 DKKIKQIRDTLEAINHDKTQFSFSERVID--TRPDELRARRDIGSFIPEEEVIGRNDDKE 180
KIK+IR+ L+ IN D+TQF SERVI+ T + LR RD G +I EEEVIGRNDDK+
Sbjct: 121 VGKIKEIRERLKVINDDRTQFHLSERVINNPTVGNALRNIRDPGDYILEEEVIGRNDDKK 180
Query: 181 NVIHRLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDV 240
VI L ++ T+E+IA+++ VGMGGLGKTTLAQSVYN + KHF+LKLWVC+SEEF+V
Sbjct: 181 -VIIDLQLSSNTRENIAVVATVGMGGLGKTTLAQSVYNDKMMAKHFELKLWVCISEEFEV 240
Query: 241 KIIIQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLL 300
K+IIQ IIE ATG+ P+SFLQM LQ +LR+ +DGK+YLLVMDDVWNE+ EKW LKR L
Sbjct: 241 KLIIQNIIEYATGEKPESFLQMALLQTKLRKLVDGKRYLLVMDDVWNEDREKWDTLKRFL 300
Query: 301 MGGAKGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLEGQEK---ELEN 360
+GG KGS+ILITTRS +VA TFD T L+ LE LN NSWLLF+ + E+ +
Sbjct: 301 IGGTKGSKILITTRSHQVANTFDPTSLHPLEELNNNNSWLLFQDRELYQIMEQGGLKTML 360
Query: 361 SDLIEIGKEIVAKLKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDY 420
S L + LK YCALFPKD+EI+KDE+I+Q AQGFIQPNDKD+L+DIGEDY
Sbjct: 361 SILKLSCNHLPPNLKQCFQYCALFPKDFEIRKDEVIMQGMAQGFIQPNDKDKLVDIGEDY 420
Query: 421 FLELLSKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLKNGIDKRTHHFSST 480
F+ELLSKSFFQ+V KD LG+I CKMHDLMHD+ACS ++KRT H S
Sbjct: 421 FMELLSKSFFQDVKKDALGDIITCKMHDLMHDIACSENDMNA-----PTLNKRTRHVSLD 480
Query: 481 WMPRKDSEKLLQRLSKARNLRTFFM-------LNYMDGWKLECDKFYHDHLRLRTLRLRL 540
++ EKL ++L + RNLRT F+ + + + +++ LRLRTL L+L
Sbjct: 481 LTSGEELEKLPKKLYETRNLRTCFLQYDLYNSIGSDNNFNKIFSSVFYNLLRLRTLGLQL 540
Query: 541 SPMDQSAKFLGSIIGTFKHLRYLSI-----TSF--------------------------- 600
+QS K L I KHLRYL I T F
Sbjct: 541 IIDNQSVKLL-KCINNLKHLRYLKIEIITYTKFFLNSISGLYNLEVLIVRCTGLKEFPRD 600
Query: 601 -----NLKNL-----------PNCITELYNLETFILQYSSRLETLPSDINNLINLRHLDL 660
NLK+L PN I EL NLE L+ ++++ LP DI NLI+L+ LDL
Sbjct: 601 IKNLTNLKHLELIGNPNIEFFPNSIIELNNLERLFLRGYNKVKELPQDIQNLIHLQRLDL 660
Query: 661 CENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLSWNKSIEFLPDS 720
N ++FLPDSITEL++L+TLIL C KLKELPRDV+N INLRHLDLS N +IEFLP S
Sbjct: 661 GRNSNIEFLPDSITELYELKTLILEECTKLKELPRDVQNLINLRHLDLS-NTAIEFLPSS 720
Query: 721 ITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGLGQLTNLQTLNL 780
ITQL NLE LLL C LKELPEDT+ LINL+HL I G ALTHMPKGLG+LT+LQ L++
Sbjct: 721 ITQLKNLETLLLYLCDKLKELPEDTRNLINLRHLSICGCKALTHMPKGLGELTHLQRLDV 780
Query: 781 FVLG-DNNGGGLNELNELNKLKGRLSIEKLQFCNVIDLQMKDKLLQLKSCLQVLWLQWS- 840
F+LG DNNGGGL EL+ LNKL G L IE LQFC+ +DLQ K LQ KS LQ L L+W+
Sbjct: 781 FMLGKDNNGGGLRELDGLNKLSGLLKIEGLQFCSTVDLQTNAKFLQRKSGLQYLQLEWND 840
Query: 841 RSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISGYKGVKLCNWLSSN--FLGCLVSI 900
SFNEPTIG D + YERVL+CL PHSNL++I I GYKGVKLCNWLSS G ++ +
Sbjct: 841 DSFNEPTIGASDYD--YERVLECLPPHSNLQQICIRGYKGVKLCNWLSSTSYSFGGMIHM 900
Query: 901 ELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEYI-GNDDSVSSSTFFPSLEELKIVRMPK 960
E S C RL HLPQFD FP+LK L DLPSIEYI GN + SSTFFPSLE I MP
Sbjct: 901 EFSSCERLRHLPQFDLFPNLKVLCLHDLPSIEYIVGNHNYPCSSTFFPSLETFIISDMPN 960
Query: 961 LKRWW-KGTTSTEST------PIKFPCLSQLEIRGCPLRILMSWHAPLKSLTISDSEVNE 1016
LKRWW KG TST+S P+ FPCLS+L I CPL ++ AP+KSL I+D E
Sbjct: 961 LKRWWRKGQTSTKSAQNRVLFPVVFPCLSELIIWKCPLLASVTCDAPMKSLEIND----E 1020
BLAST of Lag0002715 vs. NCBI nr
Match:
XP_022152874.1 (disease resistance protein RGA2-like [Momordica charantia])
HSP 1 Score: 1029.6 bits (2661), Expect = 1.8e-296
Identity = 624/1260 (49.52%), Postives = 776/1260 (61.59%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MA+SIL+++ NIIT L S A +E+ +L G+ DELDKLED LS IQAV+LDAEE+ +
Sbjct: 1 MAESILFDLAGNIITNLASFARQELALLCGVPDELDKLEDXLSDIQAVILDAEEQQYKRH 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRD--GTSMEEVRTFFSK---LKSHLK 120
A WISKLKD Y+I DL DE SYE +RRQ ++ G +VR FFS+ + LK
Sbjct: 61 AXKHWISKLKDALYDIYDLFDEISYEALRRQATSKNNRGKLCNQVRMFFSESNPIAFALK 120
Query: 121 IDKKIKQIRDTLEAINHDKTQFSFSERVIDTRPDELRARRDIGSFIPEEEVIGRNDDKEN 180
KIK+IR+ L+AI+ DKTQF+FSERV +T D LR R+ S I EEEVIGRNDDK+
Sbjct: 121 TGHKIKEIRENLKAIDTDKTQFNFSERVSNTTNDGLRISREDASLILEEEVIGRNDDKKA 180
Query: 181 VIHRLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVK 240
+I+ L N T+EDIA+ISIVG GGLGKT LAQSVYN + KHF K WVCVS+EF++K
Sbjct: 181 IINLLLKPN-TEEDIAVISIVGTGGLGKTALAQSVYNDNMMDKHFKKKFWVCVSDEFELK 240
Query: 241 IIIQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLM 300
++I+K+IESATGK P SFLQMDS Q+ELR+ IDGKKYLL+MDDVWNE++EKWV L+RLL+
Sbjct: 241 VMIEKMIESATGKRPDSFLQMDSSQSELRKLIDGKKYLLIMDDVWNEDYEKWVTLRRLLI 300
Query: 301 GGAKGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLEG-QEKELENSDL 360
GAKGSRILITTRSE+VA+TFDT+ LYSLE L+E +SWLLFKKM L+G +E++ ENS+L
Sbjct: 301 CGAKGSRILITTRSEKVAKTFDTSSLYSLEKLDEYHSWLLFKKMAFLKGSEEQDTENSNL 360
Query: 361 IEIGKEIVAKLKAM---------------------------------------------- 420
++IGKEIVAKLK +
Sbjct: 361 VQIGKEIVAKLKGVPLAIRTIGRLLCYNKKSNFWSSFKDKELYQIIEEESVKKMLSILKL 420
Query: 421 ------------LFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDYFLELL 480
YCALFPKDY+I+K+E++LQW AQGF+Q ND + +D GEDYF++LL
Sbjct: 421 SYNSLPSNLKQCFLYCALFPKDYKIRKEEIVLQWIAQGFVQSNDGVKALDAGEDYFMKLL 480
Query: 481 SKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNE--CVHGLK-NGIDKRTHHFSSTWM 540
S SFFQ+V K+++G+I CKMHDLMHDLACSI +N VH + ++KRTHH S +
Sbjct: 481 SMSFFQDVRKNDVGDIITCKMHDLMHDLACSIVENNEIIVHDVNARTLNKRTHHVSFVLL 540
Query: 541 PRKDSEKLLQRLSKARNLRTFFMLNYMDG------WKLECDK----FYHDHLRLRTLRLR 600
K+ EKL + LSKARNLRT F+ G K + +K +++ LRLRTL L
Sbjct: 541 SGKELEKLPKTLSKARNLRTCFLQYNQVGRRDDMLGKRKINKVFGSVFYNLLRLRTLDLH 600
Query: 601 LSPMDQSAKFLGSIIGTFKHLRYL-------------SITSF------------------ 660
LS + + KFL I K+LRYL SIT
Sbjct: 601 LSIDNHNVKFL-KCISKLKYLRYLKIKMIETTEYFPNSITGLYSLEVFILECKGLKELPR 660
Query: 661 ------------------------------------------------------------ 720
Sbjct: 661 DIKNWNKLKHLYLDGNENVWHRQDTYHYGNENVEFLPSSTVVLNNLETLVLQNFNNLKEF 720
Query: 721 -------------------NLKNLPNCITELYNLETFILQYSSRLETLPSDINNLINLRH 780
N++ LPN ++EL NL++ IL ++ LP DI NLI+L+H
Sbjct: 721 PEDIKNLTNLTHLVMRGNKNIEYLPNSMSELNNLKSLILTRFEKVRELPQDIKNLIHLQH 780
Query: 781 LDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLSWNKSIEFL 840
LDL N ++FLP+SITEL +LET+IL C KLKELPRDVKNWINLRHLDLS +EFL
Sbjct: 781 LDLQWNRNVEFLPNSITELCELETIILKGCTKLKELPRDVKNWINLRHLDLS-GSGVEFL 840
Query: 841 PDSITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGLGQLTNLQT 900
PDSIT NNLE LLL YC LKELP DTKKLINLKHLD+SG ALTHMPKGLG+LTNLQT
Sbjct: 841 PDSITHSNNLETLLLRYCEKLKELPGDTKKLINLKHLDLSGCRALTHMPKGLGELTNLQT 900
Query: 901 LNLFVL--GDNNGGGLNELNELNKLKGRLSIEKLQFCNVIDLQMKDKLLQLKSCLQVLWL 960
LNLF+L G+NNGGGL+ELN LNKL+G L I+ LQFC+ +DLQ KLLQLKS LQ L L
Sbjct: 901 LNLFILGKGENNGGGLSELNGLNKLRGSLIIKGLQFCSAVDLQTNAKLLQLKSGLQQLML 960
Query: 961 QW---SRSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISGYKGVKLCNWLSS---NF 1020
+W SFNEPT+G LD YERVL+CLQPHSNL EI I+GY+GVKLCNWLSS +F
Sbjct: 961 EWKWDKDSFNEPTVGALDGE--YERVLECLQPHSNLPEIHINGYRGVKLCNWLSSSSYSF 1020
Query: 1021 LGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEYIGNDDSVSSSTFFPSLEELK 1037
G LV + +S C RL+HLP+F+QFP+LK+LH DLP + ++ N+D SS T F S E L
Sbjct: 1021 DG-LVRMTVSSCERLQHLPRFEQFPNLKHLHLRDLPCVRHMVNNDYPSSPTLFQSPEVLV 1080
BLAST of Lag0002715 vs. NCBI nr
Match:
XP_031736581.1 (putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651704.1 hypothetical protein Csa_006537 [Cucumis sativus])
HSP 1 Score: 1013.8 bits (2620), Expect = 1.0e-291
Identity = 611/1165 (52.45%), Postives = 743/1165 (63.78%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPML-WGLDDELDKLEDTLSAIQAVLLDAEERPINT 60
MA++IL+N+ A+II KLGS A+R+ L G+ D+ DKL +LSAIQAVL DAEE+
Sbjct: 1 MAEAILFNLTADIIFKLGSSALRQFGSLRGGVKDDFDKLWHSLSAIQAVLHDAEEKQFKD 60
Query: 61 NAMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLKSHLKIDKK 120
+A+ W+S+LKDV YEIDDL+DEFSY+ +RRQV+ ++ ++VRT FSK ++ KI K
Sbjct: 61 HAVEVWVSRLKDVLYEIDDLIDEFSYQILRRQVL---QSNRKQVRTLFSKFITNWKIGHK 120
Query: 121 IKQIRDTLEAINHDKTQFSFSERVIDTRPDE---LRARRDIGSFIPEEEVIGRNDDKENV 180
IK+I L+ IN DK QFSF + VI+ R D+ LR RR+ SFI E+EVIGRNDDKE V
Sbjct: 121 IKEISQRLQNINEDKIQFSFCKHVIERRDDDDEGLRKRRETHSFILEDEVIGRNDDKEAV 180
Query: 181 IHRLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKI 240
I L N+N T+EDIA++SIVGM G GKT LAQ +YNH I F LK+WVCVS+EFD+KI
Sbjct: 181 IDLLLNSN-TKEDIAIVSIVGMPGFGKTALAQFIYNHKRIMTQFQLKIWVCVSDEFDLKI 240
Query: 241 IIQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMG 300
IQKIIESATGK PKS LQMD LQ ELR+QIDGKKYL+VMDDVWNE EKW+ LKRLLMG
Sbjct: 241 TIQKIIESATGKKPKSLLQMDPLQCELRKQIDGKKYLIVMDDVWNEKKEKWLHLKRLLMG 300
Query: 301 GAKGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLE----GQEKELE-- 360
GAKGSRILITTRSE+VA+TFD+TF++ L+ L+ NSWLLF+KM GLE QE EL+
Sbjct: 301 GAKGSRILITTRSEQVAKTFDSTFVHLLQILDASNSWLLFQKMIGLEEHSNNQEIELDQK 360
Query: 361 NSDLIEIGKEIV------------------------------------------------ 420
NS+LI+IG EIV
Sbjct: 361 NSNLIQIGMEIVSTLRGVPLLIRTIGGLLKDNKSERFWLSFKNKELYQVLGRGQDALKEI 420
Query: 421 -------------AKLKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPN----DKDELI 480
+ LK YCALFPKDY I+KDELIL WRAQGFIQ N D L+
Sbjct: 421 QLFLELSYKYLPSSNLKQCFLYCALFPKDYRIKKDELILLWRAQGFIQQNGNNDDNSSLV 480
Query: 481 DIGEDYFLELLSKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLK-NGIDKR 540
DIGEDYF+ELLS+SFFQEV K++ G+I CKMHDLMHDLACSIT NECV GLK N IDKR
Sbjct: 481 DIGEDYFMELLSRSFFQEVEKNDFGDIITCKMHDLMHDLACSITNNECVRGLKGNVIDKR 540
Query: 541 THHFSSTWMPRKDSEKLLQRLSKARNLRTFFMLNYMDGWKLECDKFYHDHLRLRTLRLRL 600
THH S + +D +L+ LSKA +LRT F+ + LE + +H+ +LRTL L L
Sbjct: 541 THHLSFEKVSHED--QLMGSLSKATHLRTLFIQDVCSRCNLE--ETFHNIFQLRTLHLNL 600
Query: 601 SPMDQSAKFLGSIIGTFKHLRYLSI-TSFNLKNLPNCITELYNLETFILQYSSRLETLPS 660
+ AK I KHLRYL + SF + LP+ I ELYNLETFI Q SS L+ LPS
Sbjct: 601 YSPTKFAK-TWKFISKLKHLRYLHLKNSFCVTYLPDSILELYNLETFIFQ-SSLLKKLPS 660
Query: 661 DINNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLD 720
++ NLINL+HLDL + L+FLPDSIT+L+KLE LILH
Sbjct: 661 NVGNLINLKHLDLSSHLNLEFLPDSITKLYKLEALILHG--------------------- 720
Query: 721 LSWNKSIEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPK 780
CSNLKELP+ TK+LINLK L + G ALTHMPK
Sbjct: 721 ---------------------------CSNLKELPKYTKRLINLKSLVLYGCSALTHMPK 780
Query: 781 GLGQLTNLQTLNLFVLGDNNGGGLNELNELNKLKGRLSIEKLQFC-NVIDLQMKDKLLQL 840
GL ++TNLQTL FVLG N GG L EL L KL+G LSI+ L+ C +++D QMK KLLQL
Sbjct: 781 GLSEMTNLQTLTTFVLGKNIGGELKELEGLTKLRGGLSIKHLESCTSIVDQQMKSKLLQL 840
Query: 841 KSCLQVLWLQWSRSFNEPTIGNLDNNNV-YERVLDCLQPHSNLREIRISGYKGVKLCNWL 900
KS LQ L LQW + P IG+ +V YE VLDCLQPHSNL+EIRI GY GV LCNW+
Sbjct: 841 KSGLQKLELQWKK----PKIGDDQLEDVMYESVLDCLQPHSNLKEIRIDGYGGVNLCNWV 900
Query: 901 SSN-FLGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEY--IGNDDSVSSSTFF 960
SSN LGCLV+I L C RL HL + DQFP+LKYL ++LP+IEY + NDDSVSSST F
Sbjct: 901 SSNKSLGCLVTIYLYRCKRLRHLFRLDQFPNLKYLTLQNLPNIEYMIVDNDDSVSSSTIF 960
Query: 961 PSLEELKIVRMPKLKRWWKGTTSTESTPIKFPCLSQLEIRG-CPLRILMSWHAP-LKSLT 1020
P L++ I +MPKL W K +TST+S + FP LS L IRG C L +L WHAP LK L
Sbjct: 961 PCLKKFTISKMPKLVSWCKDSTSTKSPTVIFPHLSSLMIRGPCRLHMLKYWHAPKLKLLQ 1020
Query: 1021 ISDSEVNEFDVVPWQNFENLSSLILFNFSKLEYLPKCLQH-VTTLQNLRLWDCENLKSLP 1033
ISDSE +E +VVP + +ENL+SL L N S++EYLP+C QH +T+LQ L L CENLKSLP
Sbjct: 1021 ISDSE-DELNVVPLKIYENLTSLFLHNLSRVEYLPECWQHYMTSLQLLYLSKCENLKSLP 1080
BLAST of Lag0002715 vs. ExPASy Swiss-Prot
Match:
Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)
HSP 1 Score: 433.3 bits (1113), Expect = 7.6e-120
Identity = 342/1130 (30.27%), Postives = 523/1130 (46.28%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MA++ + ++ N+ + L E+ +L+G DE +L S IQAVL DA+E+ +N
Sbjct: 1 MAEAFIQVLLDNLTSFLKG----ELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNK 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSK-LKSHLKIDKK 120
+ +W+ KL YE+DD+LDE+ + R S E + K + K+ K+
Sbjct: 61 PLENWLQKLNAATYEVDDILDEYKTKATR--------FSQSEYGRYHPKVIPFRHKVGKR 120
Query: 121 IKQIRDTLEAINHDKTQFSFSERVIDTRPDELRARRDIGSFIPEEEVIGRNDDKENVIHR 180
+ Q+ L+AI ++ F E++++ + RR+ GS + E +V GR+ +K+ ++
Sbjct: 121 MDQVMKKLKAIAEERKNFHLHEKIVERQ----AVRRETGSVLTEPQVYGRDKEKDEIVKI 180
Query: 181 LSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKIIIQ 240
L N + + ++++ I+GMGGLGKTTLAQ V+N +T+HF K+W+CVSE+FD K +I+
Sbjct: 181 LINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIK 240
Query: 241 KIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMGGAK 300
I+ES G+ + + LQ +L+ ++GK+YLLV+DDVWNE+ +KW L+ +L GA
Sbjct: 241 AIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGAS 300
Query: 301 GSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLEGQEKELENSDLIEIGK 360
G+ +L TTR E+V T Y L NL++ + WLLF M G ++E+ N +L+ IGK
Sbjct: 301 GASVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLF--MQRAFGHQEEI-NPNLVAIGK 360
Query: 361 EIVAK------------------------------------------------------- 420
EIV K
Sbjct: 361 EIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQLPL 420
Query: 421 -LKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDYFLELLSKSFFQE 480
LK YCA+FPKD +++K++LI W A GF+ EL D+G++ + EL +SFFQE
Sbjct: 421 DLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQE 480
Query: 481 VTKDELGNIKA-CKMHDLMHDLACSITKNECVHGLKNGIDKRTHHFSSTWMPRKDSEKLL 540
+ E+ + K KMHDL+HDLA S+ FS+
Sbjct: 481 I---EVKDGKTYFKMHDLIHDLATSL-------------------FSAN----------- 540
Query: 541 QRLSKARNLRTFFMLNYMDGWKLECDKFYHDHLRLRTLRLRLSPMDQSAKFLGSIIGTFK 600
+ + N+R E +K + H+
Sbjct: 541 ---TSSSNIR-------------EINKHSYTHM--------------------------M 600
Query: 601 HLRYLSITSFNLKNLPNCITELYNLETFILQYSSRLETLPSDINNLINLRHLDLCENDTL 660
+ + + F TLP + I+LR L+L ++ T
Sbjct: 601 SIGFAEVVFF--------------------------YTLP-PLEKFISLRVLNLGDS-TF 660
Query: 661 KFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLSWNKSIEFLPDSITQLNN 720
LP SI +L ++LR+L+L + + LP + +L N
Sbjct: 661 NKLPSSIGDL------------------------VHLRYLNL-YGSGMRSLPKQLCKLQN 720
Query: 721 LEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGLGQLTNLQTLNLFVLGDN 780
L+ L L YC+ L LP++T KL +L++L + G +LT MP +G LT L+TL FV+G
Sbjct: 721 LQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRK 780
Query: 781 NGGGLNELNELNKLKGRLSIEKLQFCNVIDLQMKDKLLQLKSCLQVLWLQWSRSFNEPTI 840
G L EL LN L G + I L+ D K+ L K L L + W
Sbjct: 781 KGYQLGELGNLN-LYGSIKISHLERVK-NDKDAKEANLSAKGNLHSLSMSW--------- 840
Query: 841 GNLDNNNVYE----RVLDCLQPHSNLREIRISGYKGVKLCNWLSSNFLGCLVSIELSYCI 900
N ++YE +VL+ L+PHSNL ++I G++G+ L W++ + L +VSI +S
Sbjct: 841 -NNFGPHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFR 900
Query: 901 RLEHLPQFDQFPHLKYLHFE-DLPSIEYIGNDDSVSSSTF-----FPSLEELKIVRMPKL 960
LP F P L+ L +EY+ D S F FPSL +L I L
Sbjct: 901 NCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSL 960
Query: 961 KRWWKGTTSTESTPIKFPCLSQLEIRGCPLRILMSWHAPLKSLTISDSEV-NEFDVVPWQ 1020
KG E +FP L ++ I CP L S L SL I ++V F ++
Sbjct: 961 ----KGLLKKEGEE-QFPVLEEMIIHECPFLTLSSNLRALTSLRICYNKVATSFPEEMFK 966
Query: 1021 NFENLSSLILFNFSKLEYLPKCLQHVTTLQNLRLW------------------------- 1037
N NL L + + L+ LP L + L++L++
Sbjct: 1021 NLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVE 966
BLAST of Lag0002715 vs. ExPASy Swiss-Prot
Match:
Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)
HSP 1 Score: 427.9 bits (1099), Expect = 3.2e-118
Identity = 348/1126 (30.91%), Postives = 517/1126 (45.91%), Query Frame = 0
Query: 24 EIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTNAMADWISKLKDVFYEIDDLLDEF 83
E+ +++G + E KL S IQAVL DA+E+ + A+ +W+ KL YE+DD+LD+
Sbjct: 20 ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79
Query: 84 SYETIR-RQVMIRDGTSMEEVRTFFSKLKSHLKIDKKIKQIRDTLEAINHDKTQFSFSER 143
E R +Q ++ G TF K+ K++K++ + L+AI ++ F ER
Sbjct: 80 KTEAARFKQAVL--GRYHPRTITFC------YKVGKRMKEMMEKLDAIAEERRNFHLDER 139
Query: 144 VIDTRPDELRARRDIGSFIPEEEVIGRNDDKENVIHRLSNTNTTQEDIALISIVGMGGLG 203
+I+ + ARR G + E +V GR +++ ++ L N + E++ ++ I+GMGGLG
Sbjct: 140 IIERQ----AARRQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLG 199
Query: 204 KTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKIIIQKIIESATGKSPKSFLQMDSLQNE 263
KTTLAQ V+N IT+HF+LK+WVCVS++FD K +I+ I+ES GKS + + LQ +
Sbjct: 200 KTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKS-LGDMDLAPLQKK 259
Query: 264 LRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMGGAKGSRILITTRSERVAETFDTTFLY 323
L+ ++GK+Y LV+DDVWNE+ EKW L+ +L GA G+ ILITTR E++ T LY
Sbjct: 260 LQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLEKIGSIMGTLQLY 319
Query: 324 SLENLNECNSWLLFKKMTGLEGQEKELENSDLIEIGKEIVAK------------------ 383
L NL++ + WLLFK+ E + L+EIGKEIV K
Sbjct: 320 QLSNLSQEDCWLLFKQRAFCHQTE---TSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFK 379
Query: 384 --------------------------------------LKAMLFYCALFPKDYEIQKDEL 443
L+ YCA+FPKD +I+K+ L
Sbjct: 380 REESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEYL 439
Query: 444 ILQWRAQGFIQPNDKDELIDIGEDYFLELLSKSFFQEVTKDELGNIKA-CKMHDLMHDLA 503
I W A F+ EL D+G + + EL +SFFQE+ E+ + K KMHDL+HDLA
Sbjct: 440 IALWMAHSFLLSKGNMELEDVGNEVWNELYLRSFFQEI---EVKSGKTYFKMHDLIHDLA 499
Query: 504 CSITKNECVHGLKNGIDKRTHHFSSTWMPRKDSEKLLQRLSKARNLRTFFMLNYMDGWKL 563
S+ FS++ S + Q K F + NY D +
Sbjct: 500 TSM-------------------FSAS----ASSRSIRQINVKDDEDMMFIVTNYKDMMSI 559
Query: 564 ECDKFYHDHLRLRTLRLRLSPMDQSAKFLGSIIGTFKHLRYLSITSFNLKNLPNCITELY 623
+ + SP FK R++S+ NL N
Sbjct: 560 GFSEVVSSY----------SP------------SLFK--RFVSLRVLNLSN--------- 619
Query: 624 NLETFILQYSSRLETLPSDINNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRK 683
S E LPS + +L++LR+LDL N LP + +L L+TL L+NC+
Sbjct: 620 ----------SEFEQLPSSVGDLVHLRYLDLSGNKICS-LPKRLCKLQNLQTLDLYNCQS 679
Query: 684 LKELPRDVKNWINLRHLDLSWNKSIEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLI 743
L LP+ +LR+ L+L +C
Sbjct: 680 LSCLPKQTSKLCSLRN------------------------LVLDHC-------------- 739
Query: 744 NLKHLDISGFIALTHMPKGLGQLTNLQTLNLFVLGDNNGGGLNELNELNKLKGRLSIEKL 803
LT MP +G LT L+TL FV+G+ G L EL LN L+G +SI L
Sbjct: 740 -----------PLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNLN-LRGAISITHL 799
Query: 804 QFCNVIDLQMKDKLLQLKSCLQVLWLQWSRSFNEPTIGNLDNNNVYE----RVLDCLQPH 863
+ D++ K+ L K+ L L + W D N YE +VL+ L+PH
Sbjct: 800 ERVK-NDMEAKEANLSAKANLHSLSMSW------------DRPNRYESEEVKVLEALKPH 859
Query: 864 SNLREIRISGYKGVKLCNWLSSNFLGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFED-L 923
NL+ + I + G L +W++ + L +VSI +S C LP F + P L+ L +D
Sbjct: 860 PNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESLELQDGS 919
Query: 924 PSIEYIGNDDSVSSSTFFPSLEELKIVRMPKLK--RWWKGTTSTESTPIKFPCLSQLEIR 983
+EY+ D + FPSL +L I LK + KG +FP L +++I
Sbjct: 920 VEVEYV-EDSGFLTRRRFPSLRKLHIGGFCNLKGLQRMKGAE-------QFPVLEEMKIS 979
Query: 984 GCPLRIL-------------------MSWHAPLKSLT----ISDSEVNEFDVVPWQNFEN 1037
CP+ + +S + L +LT S+ V ++N EN
Sbjct: 980 DCPMFVFPTLSSVKKLEIWGEADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLEN 988
BLAST of Lag0002715 vs. ExPASy Swiss-Prot
Match:
Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)
HSP 1 Score: 423.7 bits (1088), Expect = 6.0e-117
Identity = 341/1151 (29.63%), Postives = 524/1151 (45.53%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MA++ L ++ N+ + +G + V +++G + E +KL S IQAVL DA+E+ +
Sbjct: 1 MAEAFLQVLLENLTSFIGDKLV----LIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFF---SKLKSHLKID 120
A+ +W+ KL YE+DD+L E E IR E+ R F + KI
Sbjct: 61 AIENWLQKLNSAAYEVDDILGECKNEAIR----------FEQSRLGFYHPGIINFRHKIG 120
Query: 121 KKIKQIRDTLEAINHDKTQFSFSERVIDTRPDELRARRDIGSFIPEEEVIGRNDDKENVI 180
+++K+I + L+AI+ ++ +F F E++ T A R+ G + E +V GR+ +++ ++
Sbjct: 121 RRMKEIMEKLDAISEERRKFHFLEKI--TERQAAAATRETGFVLTEPKVYGRDKEEDEIV 180
Query: 181 HRLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKII 240
L N E++ + I+GMGGLGKTTLAQ ++N +TKHF+ K+WVCVS++FD K +
Sbjct: 181 KILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRL 240
Query: 241 IQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMGG 300
I+ II + SP + S Q +L+ ++GK+YLLV+DDVWN++ EKW L+ +L G
Sbjct: 241 IKTIIGNIERSSP-HVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVG 300
Query: 301 AKGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLEGQEKELENSDLIEI 360
A+G+ IL TTR E+V T Y L NL+ +S LLF M GQ+KE N +L+ I
Sbjct: 301 ARGASILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLF--MQRAFGQQKE-ANPNLVAI 360
Query: 361 GKEIVAK----------------------------------------------------- 420
GKEIV K
Sbjct: 361 GKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILPALRLSYHHL 420
Query: 421 ---LKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDYFLELLSKSFF 480
L+ YCA+FPKD ++ K+ LI W A GF+ EL D+G + + EL +SFF
Sbjct: 421 PLDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYLRSFF 480
Query: 481 QEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLKNGIDKRTHHFSSTWMPRKDSEKL 540
QE+ + + GN K+HDL+HDLA S+ FS++
Sbjct: 481 QEI-EAKSGN-TYFKIHDLIHDLATSL-------------------FSAS---------- 540
Query: 541 LQRLSKARNLRTFFMLNYMDGWKLECDKFYHDHLRLRTLRLRLSPMDQSAKFLGSIIGTF 600
+ N+R +N D +
Sbjct: 541 ----ASCGNIRE---INVKD---------------------------------------Y 600
Query: 601 KHLRYLSITSFNLKNLPNCITELYNLETFILQYSSRLETLPSDINNLINLRHLDLCENDT 660
KH + + P+ + + +L L Y S+LE LPS I +L++LR+LDL N+
Sbjct: 601 KHTVSIGFAAVVSSYSPSLLKKFVSLRVLNLSY-SKLEQLPSSIGDLLHLRYLDLSCNN- 660
Query: 661 LKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLSWNKSIEFLPDSITQLN 720
+ LP+ + +L L+TL +HNC L
Sbjct: 661 FRSLPERLCKLQNLQTLDVHNCYSLN---------------------------------- 720
Query: 721 NLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGLGQLTNLQTLNLFVLGD 780
C LP+ T KL +L+HL + G LT P +G LT L+TL F++G
Sbjct: 721 ---------C-----LPKQTSKLSSLRHLVVDG-CPLTSTPPRIGLLTCLKTLGFFIVGS 780
Query: 781 NNGGGLNELNELNKLKGRLSIEKLQFCNVIDLQMKDKLLQLKSCLQVLWLQWSRSFNEPT 840
G L EL LN L G +SI L+ V + + L K+ LQ L + W
Sbjct: 781 KKGYQLGELKNLN-LCGSISITHLE--RVKNDTDAEANLSAKANLQSLSMSWDN------ 840
Query: 841 IGNLDNNNVYE----RVLDCLQPHSNLREIRISGYKGVKLCNWLSSNFLGCLVSIELSYC 900
D N YE +VL+ L+PH NL+ + I + G + +W++ + L ++S+ + C
Sbjct: 841 ----DGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSC 900
Query: 901 IRLEHLPQFDQFPHLKYLHFED-LPSIEYIGNDD---SVSSSTFFPSLEELKIVRMPKLK 960
LP F + P L+ L ++ +EY+ DD S+ FPSL++L+I LK
Sbjct: 901 KNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSLK 960
Query: 961 RWWKGTTSTESTPIKFPCLSQLEIRGCPLRIL----------------------MSWHAP 1020
K E KFP L ++ I CPL + +S +
Sbjct: 961 GLMK-----EEGEEKFPMLEEMAILYCPLFVFPTLSSVKKLEVHGNTNTRGLSSISNLST 985
Query: 1021 LKSLTI-SDSEVNEFDVVPWQNFENLSSLILFNFSKLEYLPKCLQHVTTLQNLRLWDCEN 1037
L SL I ++ + + NL L F+F L+ LP L + L+ L++ C++
Sbjct: 1021 LTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSLTSLNALKRLQIESCDS 985
BLAST of Lag0002715 vs. ExPASy Swiss-Prot
Match:
Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)
HSP 1 Score: 423.3 bits (1087), Expect = 7.8e-117
Identity = 340/1143 (29.75%), Postives = 523/1143 (45.76%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MA++ + V+ N+ + L E+ +L+G DE +L S IQAVL DA+E+ +N
Sbjct: 1 MAEAFIQVVLDNLTSFLKG----ELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDK 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLKSHLKIDKKI 120
+ +W+ KL YE+DD+LDE+ + R E R + K+ K++
Sbjct: 61 PLENWLQKLNAATYEVDDILDEYKTKATR-------FLQSEYGRYHPKVIPFRHKVGKRM 120
Query: 121 KQIRDTLEAINHDKTQFSFSERVIDTRPDELRARRDIGSFIPEEEVIGRNDDKENVIHRL 180
Q+ L AI ++ +F E++I+ + A R+ GS + E +V GR+ +K+ ++ L
Sbjct: 121 DQVMKKLNAIAEERKKFHLQEKIIERQ----AATRETGSVLTEPQVYGRDKEKDEIVKIL 180
Query: 181 SNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKIIIQK 240
NT + + ++++ I+GMGGLGKTTL+Q V+N +T+ F K+W+C+S++F+ K +I+
Sbjct: 181 INTASDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKA 240
Query: 241 IIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMGGAKG 300
I+ES GKS S + + LQ +L+ ++GK+Y LV+DDVWNE+ KW L+ +L GA G
Sbjct: 241 IVESIEGKS-LSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASG 300
Query: 301 SRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLEGQEKELENSDLIEIGKE 360
+ +L TTR E+V T Y L NL+ + W LF M G ++E+ N +L+ IGKE
Sbjct: 301 AFVLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLF--MQRAFGHQEEI-NPNLMAIGKE 360
Query: 361 IVAK-------------------------------------------------------- 420
IV K
Sbjct: 361 IVKKCGGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLPLD 420
Query: 421 LKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDYFLELLSKSFFQEV 480
L+ YCA+FPKD ++ K+ LI W A GF+ EL D+G + + EL +SFFQE+
Sbjct: 421 LRQCFVYCAVFPKDTKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEI 480
Query: 481 TKDELGNIKACKMHDLMHDLACSITKNECVHGLKNGIDKRTHHFSSTWMPRKDSEKLLQR 540
+ KMHDL+HDLA S+ FS+
Sbjct: 481 EVE--SGKTYFKMHDLIHDLATSL-------------------FSAN------------- 540
Query: 541 LSKARNLRTFFMLNYMDGWKLECDKFYHDHLRLRTLRLRLSPMDQSAKFLGSIIGTFKHL 600
+ + N+R NY DG+ + + + + + S++ F L
Sbjct: 541 -TSSSNIRE-INANY-DGYMMS-----------------IGFAEVVSSYSPSLLQKFVSL 600
Query: 601 RYLSITSFNLKNLPNCITELYNLETFILQYSSRLETLPSDINNLINLRHLDLCENDTLKF 660
R L NL+N S L LPS I +L++LR+LDL N ++
Sbjct: 601 RVL-----NLRN-------------------SNLNQLPSSIGDLVHLRYLDLSGNFRIRN 660
Query: 661 LPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLSWNKSIEFLPDSITQLNNLE 720
LP + +L L+TL LH
Sbjct: 661 LPKRLCKLQNLQTLDLH------------------------------------------- 720
Query: 721 ILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGLGQLTNLQTLNLFVLGDNNG 780
YC +L LP+ T KL +L++L + G +LT P +G LT L++L+ FV+G G
Sbjct: 721 -----YCDSLSCLPKQTSKLGSLRNLLLDG-CSLTSTPPRIGLLTCLKSLSCFVIGKRKG 780
Query: 781 GGLNELNELNKLKGRLSIEKLQFCNVIDLQMKDKLLQLKSCLQVLWLQWSRSFNEPTIGN 840
L EL LN L G +SI KL D K+ L K+ L L L W +
Sbjct: 781 HQLGELKNLN-LYGSISITKLDRVKK-DTDAKEANLSAKANLHSLCLSW----------D 840
Query: 841 LDNNNVYE-RVLDCLQPHSNLREIRISGYKGVKLCNWLSSNFLGCLVSIELSYCIRLEHL 900
LD + Y+ VL+ L+PHSNL+ + I+G+ G++L +W++ + L +VSI + C L
Sbjct: 841 LDGKHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCL 900
Query: 901 PQFDQFPHLKYLHFE-DLPSIEYIGNDDSVSSSTFFPSLEELKIVRMPKLKRWWKGTTST 960
P F + P L+ L +EY+ +D+V FPSL +L I L KG
Sbjct: 901 PPFGELPCLESLELHTGSADVEYV--EDNVHPGR-FPSLRKLVIWDFSNL----KGLLKM 960
Query: 961 ESTPIKFPCLSQLEIRGCPLRIL---------------------MSWHAPLKSLTISDS- 1020
E +FP L ++ CP+ ++ +S L SL ISD+
Sbjct: 961 EGEK-QFPVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRSISNLRALTSLDISDNV 977
Query: 1021 EVNEFDVVPWQNFENLSSLILFNFSKLEYLPKCL-------------------------Q 1039
E +++ NL L + F L+ LP L +
Sbjct: 1021 EATSLPEEMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEEGVK 977
BLAST of Lag0002715 vs. ExPASy Swiss-Prot
Match:
Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 349.0 bits (894), Expect = 1.9e-94
Identity = 303/1116 (27.15%), Postives = 491/1116 (44.00%), Query Frame = 0
Query: 35 LDKLEDTLSAIQAVLLDAEERPINTNAMADWISKLKDVFYEIDDLLDEFSYETIRRQVMI 94
L++L L I AVL+DAEE+ I + W+++L+DV Y +D LD+ + E +R +
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIG- 98
Query: 95 RDGTSMEEVRTFFSKLKSHLKIDKKIKQIRDTLEAINHDKTQFSFSERVIDTRPDELRA- 154
+ +S +R ++ +D + + LE + + + ++ + EL A
Sbjct: 99 AESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLK--ELTAM 158
Query: 155 ----RRDIGSFIPEEEVIGRNDDKENVIHRLSNTNTTQEDIALISIVGMGGLGKTTLAQS 214
R S + E EV GR+DDK+ ++ L N I +++IVG+GG+GKTTL+Q
Sbjct: 159 IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQL 218
Query: 215 VYNHPNITKHFDLKLWVCVSEEFDVKIIIQKIIESATGKSPKSFLQMDSLQNELRRQIDG 274
+YN ++ +F K+W VSEEFDV I +K+ ES T + P F +D LQ +L+ ++ G
Sbjct: 219 LYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSR-PCEFTDLDVLQVKLKERLTG 278
Query: 275 K--KYLLVMDDVWNENHEKWVCLKRLLMGGAKGSRILITTRSERVAETFDTTFLYSLENL 334
+LLV+DD+WNEN W L++ + A+GS+IL+TTRS+RVA +++L+ L
Sbjct: 279 TGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQPL 338
Query: 335 NECNSWLLFKKMTGLEGQEKELENSDLIEIGKEIV------------------------- 394
++ + W LF K + G ++ N ++ ++ + IV
Sbjct: 339 SDGDCWSLFMKT--VFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIE 398
Query: 395 -------------------------------AKLKAMLFYCALFPKDYEIQKDELILQWR 454
A LK YC++FPK + +KD+++L W
Sbjct: 399 WERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWM 458
Query: 455 AQGFI-QPNDKDELIDIGEDYFLELLSKSFFQEVTKDELGNIKACKMHDLMHDLACSIT- 514
A+GF+ Q L ++G +YF EL S+S Q+ + MHD +++LA +
Sbjct: 459 AEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRYI-------MHDFINELAQFASG 518
Query: 515 --KNECVHGLKNGIDKRTHHFSSTWMPRKDSEKL-LQRLSKARNLRTFFMLNYMDGWKLE 574
++ G K + +RT + S ++ +E + + L + + LRTF L+ + +
Sbjct: 519 EFSSKFEDGCKLQVSERTRYLS--YLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSC 578
Query: 575 CDKFYHDHLRLRTLRLRLSPMDQSAKFLGSIIGTFKHLRYLSITSFNLKNLPNCITELYN 634
C +DQ ++ T LR LS++ + + LP
Sbjct: 579 C-------------------LDQMVS--EKLLPTLTRLRVLSLSHYKIARLP-------- 638
Query: 635 LETFILQYSSRLETLPSDINNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKL 694
P N+ + R LDL S TEL K
Sbjct: 639 ---------------PDFFKNISHARFLDL-----------SRTELEK------------ 698
Query: 695 KELPRDVKNWINLRHLDLSWNKSIEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLIN 754
LP S+ + NL+ LLL+YCS+LKELP D LIN
Sbjct: 699 --------------------------LPKSLCYMYNLQTLLLSYCSSLKELPTDISNLIN 758
Query: 755 LKHLDISGFIALTHMPKGLGQLTNLQTLNLFVLGDNNGGGLNELNELNKLKGRLSIEKLQ 814
L++LD+ G L MP+ G+L +LQTL F + ++G ++EL L+ L G+L I +LQ
Sbjct: 759 LRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQ 818
Query: 815 FCNVIDL-QMKDKLLQLKSCLQ---VLWLQWSRSFNEPTIGNLDNNNVYERVLDCLQPHS 874
V+D+ + L K L+ +W S S T + N V + L+PH
Sbjct: 819 --RVVDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEA--EVFEKLRPHR 878
Query: 875 NLREIRISGYKGVKLCNWLSSNFLGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPS 934
++ ++ I YKG + +WLS +V I L C LP Q P LK LH +
Sbjct: 879 HIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVG 938
Query: 935 IEYIGN-----------------------------------DDSVSSSTFFPSLEELKIV 994
++ IG D V+ FPSL++L I+
Sbjct: 939 LQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFIL 998
Query: 995 RMPKLKRWWKGTTSTESTPIKFPCLSQLEIRGCPLRILMSWH-----APLKSLTISDSEV 1036
R P+L T + P P L L I C L H L++L+I S
Sbjct: 999 RCPEL---------TGTLPTFLPSLISLHIYKCGLLDFQPDHHEYSYRNLQTLSIK-SSC 1029
BLAST of Lag0002715 vs. ExPASy TrEMBL
Match:
A0A6J1DKY9 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111021874 PE=4 SV=1)
HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 630/1171 (53.80%), Postives = 784/1171 (66.95%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MADSIL+N+ +I KLG +E+ +L G++ ELDKL+ T+SAI VLLDAEER ++
Sbjct: 1 MADSILFNLAGTLINKLGXYVHQELGLLLGVNGELDKLKGTVSAIXKVLLDAEERSSKSH 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLK----SHLKI 120
A+ DW+SKLK VFYEIDDL+DEF+YE +RRQVM R+ ++VR FFSK SH K+
Sbjct: 61 ALEDWLSKLKGVFYEIDDLIDEFNYEALRRQVMTRNRRKTKQVRIFFSKSNQIAFSH-KM 120
Query: 121 DKKIKQIRDTLEAINHDKTQFSFSERVID-TRPDELRARRDIGSFIPEEEVIGRNDDKEN 180
KIK+IR+ L+ IN DKTQF SE VI+ + D LR RD G +IPEEEVIGRN +K+
Sbjct: 121 GGKIKEIREKLKVINDDKTQFKLSECVINKSDVDILRNTRDPGDYIPEEEVIGRNYEKKE 180
Query: 181 VIHRLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVK 240
+I L N+N +EDIA+++IVGMGGLGKTT+AQSVY+ +TKHF+L+LWVC+SEEF+VK
Sbjct: 181 IIDLLLNSN-AREDIAVVAIVGMGGLGKTTIAQSVYSDKMMTKHFELRLWVCISEEFEVK 240
Query: 241 IIIQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLM 300
+IIQ IIE AT + +SF QM LQ ELR+ +DGKKYLLVMDDVWNE+HEKW LKRLLM
Sbjct: 241 VIIQNIIEYAT-RRKESFPQMALLQTELRKLVDGKKYLLVMDDVWNEDHEKWNTLKRLLM 300
Query: 301 GGAKGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMT-GLEGQEKELENSDL 360
GGAKGSRILITTR+ +VA TFD+T YSLE LN+ NSWLLF+KM G EG EK+ ENS L
Sbjct: 301 GGAKGSRILITTRTHQVANTFDSTSXYSLEELNDKNSWLLFEKMAFGGEGAEKDPENSKL 360
Query: 361 IEIGKEIVAKLKAM---------------------------------------------- 420
+EIGKEIV KLK +
Sbjct: 361 VEIGKEIVTKLKGIPLAIRTVGRLLYYKKSTEFWSSFKDKKLYQIIEQGSLKDMLSILML 420
Query: 421 ------------LFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDYFLELL 480
YCA+FPKDYEIQK+E+I+QW AQ FI PNDK + IDIGE+YF+ELL
Sbjct: 421 SYNHLPYNLKQCFLYCAMFPKDYEIQKNEVIMQWIAQSFIHPNDKYKFIDIGEEYFMELL 480
Query: 481 SKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLK-NGIDKRTHHFSSTWMPR 540
S+SFFQ+VTK++LG+I CKMHDLMHDLACSI +NECVH + + KRTHH S M
Sbjct: 481 SRSFFQDVTKNDLGDIITCKMHDLMHDLACSIVENECVHDVNARTLSKRTHHVSFDLMSW 540
Query: 541 KDSEKLLQRLSKARNLRT-FFMLNYM---DGWKLECDKFYHDHLRLRTLRLRLSPMD-QS 600
++ E+L + LS+A+NLRT FF N + + + +++ LRLRTL L+L D Q+
Sbjct: 541 EELERLPETLSEAKNLRTCFFHYNQRRTDNNFNEIFESVFYNLLRLRTLDLQLLSFDKQN 600
Query: 601 AKFLGSIIGTFKHLRYLSITSF-NLKNLPNCITELYNLETFILQYSSRLETLPSDINNLI 660
KFL I KHLRYL I + + N IT LYNLE FIL+ +RL+ LP DI N
Sbjct: 601 VKFL-KCISKLKHLRYLEIQMYTKTEFFMNHITGLYNLEVFILR-CTRLKELPRDIKNWN 660
Query: 661 NLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLSWNKS 720
L+H+ L +N+ ++FLP+S EL+ LETL+LH C KLKEL DV+NWINLR+LDLS +
Sbjct: 661 ELKHVYLYKNEIIEFLPNSFAELNNLETLVLHQCTKLKELLGDVRNWINLRYLDLS-SSG 720
Query: 721 IEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGLGQLT 780
+EFLPDSITQL NLE LLL +CS L+ELP DTK LINLKH+D+ G ALTHMPKGLG+LT
Sbjct: 721 VEFLPDSITQLKNLETLLLRFCSKLQELPRDTKNLINLKHVDLYGCWALTHMPKGLGELT 780
Query: 781 NLQTLNLFVL-GDNNGGGLNELNELNKLKGRLSIEKLQFCNVIDLQMKDKLLQLKSCLQV 840
+LQ LN FVL DNNGGGL+ELNELNKL+G L+I +L FC DL K L LKS LQ
Sbjct: 781 DLQILNFFVLEKDNNGGGLSELNELNKLRGSLTINRLNFCTTSDLPTNAKFLLLKSSLQE 840
Query: 841 LWLQW---SRSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISGYKGVKLCNWLSSNF 900
L L+W + S NEPTIG+LD++ E VL+CL PHSNLR + I Y+GVKLCNW+SSNF
Sbjct: 841 LVLEWEWDNDSVNEPTIGDLDDD--CESVLECLHPHSNLRNLCIDKYRGVKLCNWVSSNF 900
Query: 901 LGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEYIGNDDSVSSSTFFPSLEELK 960
LGCLV+I+LS C RL+HLPQFDQFP+LK L +L IEYI NDD SST FPSLEEL
Sbjct: 901 LGCLVTIKLSGCKRLQHLPQFDQFPNLKRLSLRELSGIEYIFNDDYRCSSTLFPSLEELS 960
Query: 961 IVRMPKLKRWWKGTTSTEST-------PIKFPCLSQLEIRGCPLRILMSWHAPLKSLTIS 1020
I MP LKRWWK ++++T PI FPCLS+LEI GCPL ++ HAP KSL ++
Sbjct: 961 IFCMPNLKRWWKKGQTSKATSSQNVLFPIIFPCLSKLEIWGCPLLASITCHAPPKSLEMN 1020
Query: 1021 DS-----EVNEFDVVPWQNFENLSSLILFNFSKLEYLPKCLQHVTTLQNLRLWDCENLKS 1037
D + +E DVV W+ FE+LS L L + + +L LQHVTTLQ L L C+NL +
Sbjct: 1021 DPIEFHFDPDESDVVSWKCFESLSYLRLTHIDEWGFLSNWLQHVTTLQELTLEYCQNLTN 1080
BLAST of Lag0002715 vs. ExPASy TrEMBL
Match:
A0A1S4E349 (putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499944 PE=4 SV=1)
HSP 1 Score: 1071.6 bits (2770), Expect = 1.9e-309
Identity = 606/1117 (54.25%), Postives = 756/1117 (67.68%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MA++ILYN A+II LGS A++EI LW ++ EL KL+ +LSAIQAVL DAEE+ N
Sbjct: 1 MAEAILYNFTADIIFTLGSSALQEIGSLWRVNSELHKLKHSLSAIQAVLHDAEEQQSKNN 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLKSHLKIDKKI 120
+ DW+ KL+DV YEIDDL+DE SY+T+RRQV+ + ++VR FSK KS+ KI KI
Sbjct: 61 QVKDWVLKLQDVLYEIDDLIDESSYQTLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHKI 120
Query: 121 KQIRDTLEAINHDKTQFSFSERVIDTRPDE--LRARRDIGSFIPEEEVIGRNDDKENVIH 180
K+IR L+AIN DK QFSF + VI+ R D+ LR RR+ SFI E+EVIGRNDDKE VI
Sbjct: 121 KEIRQRLQAINEDKNQFSFRKHVIERRDDDEGLRKRRETHSFILEDEVIGRNDDKEAVID 180
Query: 181 RLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKIII 240
L N+N T+EDIA++SIVGMGGLGKT LA+S+YNH I F LKLWVCVS+ FD++II+
Sbjct: 181 LLLNSN-TKEDIAIVSIVGMGGLGKTALARSIYNHKRIMTRFSLKLWVCVSDAFDLEIIV 240
Query: 241 QKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMGGA 300
QKIIESATGK +LQ+DSLQ ELR+ IDG KYL VMDDVWNE E+W+ LK LLM GA
Sbjct: 241 QKIIESATGKK-SGYLQIDSLQCELRKHIDGNKYLFVMDDVWNEKKEEWLHLKELLMAGA 300
Query: 301 KGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLE----GQEKELE--NS 360
KGSRILITTRSE+VA+TFD+TF++ L+ L+ NSWLLF+KMTGLE QE E++ N
Sbjct: 301 KGSRILITTRSEQVAKTFDSTFVHPLQILDASNSWLLFQKMTGLEELSNNQEAEIDQTNL 360
Query: 361 DLIEIGKEIVAKLKAM-------------------------------------------- 420
+LI+IGKEI+++LK +
Sbjct: 361 NLIQIGKEILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVLRQGQDALKEMKY 420
Query: 421 ----------------LFYCALFPKDYEIQKDELILQWRAQGFIQPN-DKD-ELIDIGED 480
YCALFPKD+E+ K LIL WR+QG+IQPN +KD L+DIG+
Sbjct: 421 ILELSYKYLPANLKQCFLYCALFPKDFEVPKISLILLWRSQGYIQPNGNKDNNLVDIGDG 480
Query: 481 YFLELLSKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLK-NGIDKRTHHFS 540
YF+ELLS+SFFQ+V KD+ G+I ACKMHD+MHDLACS+T NECVHGLK N I+KRT HF
Sbjct: 481 YFMELLSRSFFQDVKKDDSGDIVACKMHDMMHDLACSLTNNECVHGLKGNIINKRTRHFV 540
Query: 541 STWMPRKD--SEKLLQRLSKARNLRTFFMLNYMD--GWKLECDKFYHDHLRLRTLRLRLS 600
+ KD ++L++ LSKA +LRT F D G + ++ + R RT+ L
Sbjct: 541 ---LEDKDYHEDQLMRPLSKATHLRTLFYRKEEDVIGSQWNFEETFRSIFRFRTVHLEGY 600
Query: 601 PMDQSAKFLGSIIGTFKHLRYLSITSFNLKNLPNCITELYNLETFILQYSSRLETLPSDI 660
+ AK L IG FKHLR+LSIT N+ LP+ IT+LY LETF Q S LE LP+D+
Sbjct: 601 DQNPKAKAL-EFIGKFKHLRFLSITYSNIPYLPDSITKLYYLETFFFQCSHNLENLPNDV 660
Query: 661 NNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLS 720
NLINL+HLDL N TL+FLPDSITEL KLE LIL C KLK LP+++KN NLR LDLS
Sbjct: 661 GNLINLKHLDLSHNSTLEFLPDSITELCKLEALILKGCEKLKRLPKNIKNCNNLRQLDLS 720
Query: 721 WNKSIEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGL 780
N+ IEFLPDSITQL NLE L+L++C NLKELPEDTK+LINLK L + ALTHMPKGL
Sbjct: 721 HNERIEFLPDSITQLINLETLILSWCINLKELPEDTKRLINLKQLYLEICTALTHMPKGL 780
Query: 781 GQLTNLQTLNLFVLGDNNGGGLNELNELNKLKGRLSIEKLQFC-NVIDLQMKDKLLQLKS 840
G+LTNLQTLN FVLG+N GG L EL+ L KL+G+LSIE L+FC N++D +MK K LQLKS
Sbjct: 781 GELTNLQTLNTFVLGNNKGGELKELDGLTKLRGKLSIEHLEFCTNIVDHKMKGKFLQLKS 840
Query: 841 CLQVLWLQWSRSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISGYKGVKLCNW-LSS 900
LQ L L W + TIG L+ + +YE VLDCLQPHSNL++I I GY GV LCNW +S
Sbjct: 841 GLQELKLHWEKH----TIGELE-DVIYESVLDCLQPHSNLQKIYIDGYGGVNLCNWVVSP 900
Query: 901 NFLGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEYI--GNDDSVSSSTFFPSL 960
FLGCLV++ L C RL HLP+FDQFP+LK+L+ DLP+IEYI NDDSVSSST FPSL
Sbjct: 901 KFLGCLVTMRLCNCERLRHLPKFDQFPNLKHLYLIDLPNIEYIIANNDDSVSSSTNFPSL 960
Query: 961 EELKIVRMPKLKRWWKGTTSTESTPIKFPCLSQLEIRGCPLRILMSWHAPLKSLTISDSE 1020
E L I+ MPKL W KGTTST+S+ K
Sbjct: 961 ENLIILNMPKLVSWCKGTTSTKSSITK--------------------------------- 1020
Query: 1021 VNEFDVVPWQNFENLSSLILFNFSKLEYLPKCLQHVTTLQNLRLWDCENLKSLPEWMDNL 1039
+ + P+ +L SL+L N L+ LP+ + ++T+L +L + C+ L LPE + +
Sbjct: 1021 -SSIIIFPYHYMISLQSLLLKNCQNLKSLPEWIGNLTSLTSLYISFCKKLTMLPEGIRHT 1072
BLAST of Lag0002715 vs. ExPASy TrEMBL
Match:
A0A6J1DPI9 (LOW QUALITY PROTEIN: disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111021898 PE=4 SV=1)
HSP 1 Score: 1061.6 bits (2744), Expect = 2.1e-306
Identity = 610/1091 (55.91%), Postives = 752/1091 (68.93%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MADSIL+N+ +ITKLGS A++E+ +L G++DEL+KL+DT+SAI+AVLLDAEE+P ++
Sbjct: 1 MADSILFNLAGTLITKLGSYALQELGLLLGVNDELEKLKDTVSAIEAVLLDAEEQPSKSH 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLK----SHLKI 120
A+ DW+SKLKDVFYEIDDL+DEF YE +RRQVM R+ T ++VR FFSK SH K+
Sbjct: 61 ALKDWLSKLKDVFYEIDDLIDEFEYEALRRQVMTRNRTKTKQVRIFFSKSNQIAFSH-KM 120
Query: 121 DKKIKQIRDTLEAINHDKTQFSFSERVID--TRPDELRARRDIGSFIPEEEVIGRNDDKE 180
KIK+IR+ L+ IN D+TQF SERVI+ T + LR RD G +I EEEVIGRNDDK+
Sbjct: 121 VGKIKEIRERLKVINDDRTQFHLSERVINNPTVGNALRNIRDPGDYILEEEVIGRNDDKK 180
Query: 181 NVIHRLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDV 240
VI L ++ T+E+IA+++ VGMGGLGKTTLAQSVYN + KHF+LKLWVC+SEEF+V
Sbjct: 181 -VIIDLQLSSNTRENIAVVATVGMGGLGKTTLAQSVYNDKMMAKHFELKLWVCISEEFEV 240
Query: 241 KIIIQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLL 300
K+IIQ IIE ATG+ P+SFLQM LQ +LR+ +DGK+YLLVMDDVWNE+ EKW LKR L
Sbjct: 241 KLIIQNIIEYATGEKPESFLQMALLQTKLRKLVDGKRYLLVMDDVWNEDREKWDTLKRFL 300
Query: 301 MGGAKGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLEGQEK---ELEN 360
+GG KGS+ILITTRS +VA TFD T L+ LE LN NSWLLF+ + E+ +
Sbjct: 301 IGGTKGSKILITTRSHQVANTFDPTSLHPLEELNNNNSWLLFQDRELYQIMEQGGLKTML 360
Query: 361 SDLIEIGKEIVAKLKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDY 420
S L + LK YCALFPKD+EI+KDE+I+Q AQGFIQPNDKD+L+DIGEDY
Sbjct: 361 SILKLSCNHLPPNLKQCFQYCALFPKDFEIRKDEVIMQGMAQGFIQPNDKDKLVDIGEDY 420
Query: 421 FLELLSKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLKNGIDKRTHHFSST 480
F+ELLSKSFFQ+V KD LG+I CKMHDLMHD+ACS ++KRT H S
Sbjct: 421 FMELLSKSFFQDVKKDALGDIITCKMHDLMHDIACSENDMNA-----PTLNKRTRHVSLD 480
Query: 481 WMPRKDSEKLLQRLSKARNLRTFFM-------LNYMDGWKLECDKFYHDHLRLRTLRLRL 540
++ EKL ++L + RNLRT F+ + + + +++ LRLRTL L+L
Sbjct: 481 LTSGEELEKLPKKLYETRNLRTCFLQYDLYNSIGSDNNFNKIFSSVFYNLLRLRTLGLQL 540
Query: 541 SPMDQSAKFLGSIIGTFKHLRYLSI-----TSF--------------------------- 600
+QS K L I KHLRYL I T F
Sbjct: 541 IIDNQSVKLL-KCINNLKHLRYLKIEIITYTKFFLNSISGLYNLEVLIVRCTGLKEFPRD 600
Query: 601 -----NLKNL-----------PNCITELYNLETFILQYSSRLETLPSDINNLINLRHLDL 660
NLK+L PN I EL NLE L+ ++++ LP DI NLI+L+ LDL
Sbjct: 601 IKNLTNLKHLELIGNPNIEFFPNSIIELNNLERLFLRGYNKVKELPQDIQNLIHLQRLDL 660
Query: 661 CENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLSWNKSIEFLPDS 720
N ++FLPDSITEL++L+TLIL C KLKELPRDV+N INLRHLDLS N +IEFLP S
Sbjct: 661 GRNSNIEFLPDSITELYELKTLILEECTKLKELPRDVQNLINLRHLDLS-NTAIEFLPSS 720
Query: 721 ITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGLGQLTNLQTLNL 780
ITQL NLE LLL C LKELPEDT+ LINL+HL I G ALTHMPKGLG+LT+LQ L++
Sbjct: 721 ITQLKNLETLLLYLCDKLKELPEDTRNLINLRHLSICGCKALTHMPKGLGELTHLQRLDV 780
Query: 781 FVLG-DNNGGGLNELNELNKLKGRLSIEKLQFCNVIDLQMKDKLLQLKSCLQVLWLQWS- 840
F+LG DNNGGGL EL+ LNKL G L IE LQFC+ +DLQ K LQ KS LQ L L+W+
Sbjct: 781 FMLGKDNNGGGLRELDGLNKLSGLLKIEGLQFCSTVDLQTNAKFLQRKSGLQYLQLEWND 840
Query: 841 RSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISGYKGVKLCNWLSSN--FLGCLVSI 900
SFNEPTIG D + YERVL+CL PHSNL++I I GYKGVKLCNWLSS G ++ +
Sbjct: 841 DSFNEPTIGASDYD--YERVLECLPPHSNLQQICIRGYKGVKLCNWLSSTSYSFGGMIHM 900
Query: 901 ELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEYI-GNDDSVSSSTFFPSLEELKIVRMPK 960
E S C RL HLPQFD FP+LK L DLPSIEYI GN + SSTFFPSLE I MP
Sbjct: 901 EFSSCERLRHLPQFDLFPNLKVLCLHDLPSIEYIVGNHNYPCSSTFFPSLETFIISDMPN 960
Query: 961 LKRWW-KGTTSTEST------PIKFPCLSQLEIRGCPLRILMSWHAPLKSLTISDSEVNE 1016
LKRWW KG TST+S P+ FPCLS+L I CPL ++ AP+KSL I+D E
Sbjct: 961 LKRWWRKGQTSTKSAQNRVLFPVVFPCLSELIIWKCPLLASVTCDAPMKSLEIND----E 1020
BLAST of Lag0002715 vs. ExPASy TrEMBL
Match:
A0A6J1DHD4 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020497 PE=4 SV=1)
HSP 1 Score: 1029.6 bits (2661), Expect = 8.8e-297
Identity = 624/1260 (49.52%), Postives = 776/1260 (61.59%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MA+SIL+++ NIIT L S A +E+ +L G+ DELDKLED LS IQAV+LDAEE+ +
Sbjct: 1 MAESILFDLAGNIITNLASFARQELALLCGVPDELDKLEDXLSDIQAVILDAEEQQYKRH 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRD--GTSMEEVRTFFSK---LKSHLK 120
A WISKLKD Y+I DL DE SYE +RRQ ++ G +VR FFS+ + LK
Sbjct: 61 AXKHWISKLKDALYDIYDLFDEISYEALRRQATSKNNRGKLCNQVRMFFSESNPIAFALK 120
Query: 121 IDKKIKQIRDTLEAINHDKTQFSFSERVIDTRPDELRARRDIGSFIPEEEVIGRNDDKEN 180
KIK+IR+ L+AI+ DKTQF+FSERV +T D LR R+ S I EEEVIGRNDDK+
Sbjct: 121 TGHKIKEIRENLKAIDTDKTQFNFSERVSNTTNDGLRISREDASLILEEEVIGRNDDKKA 180
Query: 181 VIHRLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVK 240
+I+ L N T+EDIA+ISIVG GGLGKT LAQSVYN + KHF K WVCVS+EF++K
Sbjct: 181 IINLLLKPN-TEEDIAVISIVGTGGLGKTALAQSVYNDNMMDKHFKKKFWVCVSDEFELK 240
Query: 241 IIIQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLM 300
++I+K+IESATGK P SFLQMDS Q+ELR+ IDGKKYLL+MDDVWNE++EKWV L+RLL+
Sbjct: 241 VMIEKMIESATGKRPDSFLQMDSSQSELRKLIDGKKYLLIMDDVWNEDYEKWVTLRRLLI 300
Query: 301 GGAKGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLEG-QEKELENSDL 360
GAKGSRILITTRSE+VA+TFDT+ LYSLE L+E +SWLLFKKM L+G +E++ ENS+L
Sbjct: 301 CGAKGSRILITTRSEKVAKTFDTSSLYSLEKLDEYHSWLLFKKMAFLKGSEEQDTENSNL 360
Query: 361 IEIGKEIVAKLKAM---------------------------------------------- 420
++IGKEIVAKLK +
Sbjct: 361 VQIGKEIVAKLKGVPLAIRTIGRLLCYNKKSNFWSSFKDKELYQIIEEESVKKMLSILKL 420
Query: 421 ------------LFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDYFLELL 480
YCALFPKDY+I+K+E++LQW AQGF+Q ND + +D GEDYF++LL
Sbjct: 421 SYNSLPSNLKQCFLYCALFPKDYKIRKEEIVLQWIAQGFVQSNDGVKALDAGEDYFMKLL 480
Query: 481 SKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNE--CVHGLK-NGIDKRTHHFSSTWM 540
S SFFQ+V K+++G+I CKMHDLMHDLACSI +N VH + ++KRTHH S +
Sbjct: 481 SMSFFQDVRKNDVGDIITCKMHDLMHDLACSIVENNEIIVHDVNARTLNKRTHHVSFVLL 540
Query: 541 PRKDSEKLLQRLSKARNLRTFFMLNYMDG------WKLECDK----FYHDHLRLRTLRLR 600
K+ EKL + LSKARNLRT F+ G K + +K +++ LRLRTL L
Sbjct: 541 SGKELEKLPKTLSKARNLRTCFLQYNQVGRRDDMLGKRKINKVFGSVFYNLLRLRTLDLH 600
Query: 601 LSPMDQSAKFLGSIIGTFKHLRYL-------------SITSF------------------ 660
LS + + KFL I K+LRYL SIT
Sbjct: 601 LSIDNHNVKFL-KCISKLKYLRYLKIKMIETTEYFPNSITGLYSLEVFILECKGLKELPR 660
Query: 661 ------------------------------------------------------------ 720
Sbjct: 661 DIKNWNKLKHLYLDGNENVWHRQDTYHYGNENVEFLPSSTVVLNNLETLVLQNFNNLKEF 720
Query: 721 -------------------NLKNLPNCITELYNLETFILQYSSRLETLPSDINNLINLRH 780
N++ LPN ++EL NL++ IL ++ LP DI NLI+L+H
Sbjct: 721 PEDIKNLTNLTHLVMRGNKNIEYLPNSMSELNNLKSLILTRFEKVRELPQDIKNLIHLQH 780
Query: 781 LDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLRHLDLSWNKSIEFL 840
LDL N ++FLP+SITEL +LET+IL C KLKELPRDVKNWINLRHLDLS +EFL
Sbjct: 781 LDLQWNRNVEFLPNSITELCELETIILKGCTKLKELPRDVKNWINLRHLDLS-GSGVEFL 840
Query: 841 PDSITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALTHMPKGLGQLTNLQT 900
PDSIT NNLE LLL YC LKELP DTKKLINLKHLD+SG ALTHMPKGLG+LTNLQT
Sbjct: 841 PDSITHSNNLETLLLRYCEKLKELPGDTKKLINLKHLDLSGCRALTHMPKGLGELTNLQT 900
Query: 901 LNLFVL--GDNNGGGLNELNELNKLKGRLSIEKLQFCNVIDLQMKDKLLQLKSCLQVLWL 960
LNLF+L G+NNGGGL+ELN LNKL+G L I+ LQFC+ +DLQ KLLQLKS LQ L L
Sbjct: 901 LNLFILGKGENNGGGLSELNGLNKLRGSLIIKGLQFCSAVDLQTNAKLLQLKSGLQQLML 960
Query: 961 QW---SRSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISGYKGVKLCNWLSS---NF 1020
+W SFNEPT+G LD YERVL+CLQPHSNL EI I+GY+GVKLCNWLSS +F
Sbjct: 961 EWKWDKDSFNEPTVGALDGE--YERVLECLQPHSNLPEIHINGYRGVKLCNWLSSSSYSF 1020
Query: 1021 LGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEYIGNDDSVSSSTFFPSLEELK 1037
G LV + +S C RL+HLP+F+QFP+LK+LH DLP + ++ N+D SS T F S E L
Sbjct: 1021 DG-LVRMTVSSCERLQHLPRFEQFPNLKHLHLRDLPCVRHMVNNDYPSSPTLFQSPEVLV 1080
BLAST of Lag0002715 vs. ExPASy TrEMBL
Match:
A0A5A7UTU8 (Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00660 PE=4 SV=1)
HSP 1 Score: 996.1 bits (2574), Expect = 1.1e-286
Identity = 561/984 (57.01%), Postives = 688/984 (69.92%), Query Frame = 0
Query: 1 MADSILYNVVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTN 60
MA++ILYN A+II KLGS A++E+ LW ++ EL KL+ +LSAIQAVL DAEE+ N
Sbjct: 1 MAEAILYNFTADIIFKLGSSALQELGSLWRVNSELHKLKHSLSAIQAVLHDAEEQQSKNN 60
Query: 61 AMADWISKLKDVFYEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLKSHLKIDKKI 120
+ DW+ KL+DV YEIDDL+DE SYET+RRQV+ + ++VR FSK KS+ KI KI
Sbjct: 61 QVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHKI 120
Query: 121 KQIRDTLEAINHDKTQFSFSERVIDTRPD--ELRARRDIGSFIPEEEVIGRNDDKENVIH 180
K+IR+ L+AIN DK QF F + VI+ R D LR RR+ SFIP++EV+GRNDDKE VI
Sbjct: 121 KEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVID 180
Query: 181 RLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYNHPNITK--HFDLKLWVCVSEEFDVKI 240
L N+N T+EDIA++SIVGMGGLGKT LAQS+YNH ++T F +KLWVCVSEEFD+KI
Sbjct: 181 LLLNSN-TKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKI 240
Query: 241 IIQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMG 300
IIQKIIESAT K PKSFLQ+DSLQ+ELR+QIDGKKYLLVMDDVWNE E+W+ LK+LLMG
Sbjct: 241 IIQKIIESATKKKPKSFLQIDSLQHELRKQIDGKKYLLVMDDVWNEKKEEWLNLKKLLMG 300
Query: 301 GAKGSRILITTRSERVAETFDTTFLYSLENLNECNSWLLFKKMTGLEG----QEKELE-- 360
GAKGSRILITTRSE+VA+TFD+TF++ L+ L+ NSWLLF+KMTGLEG QE EL+
Sbjct: 301 GAKGSRILITTRSEQVAKTFDSTFVHLLQILDTSNSWLLFQKMTGLEGRLINQEAELDQK 360
Query: 361 NSDLIEIGKEIV------------------------------------------------ 420
NS+LI+IG EIV
Sbjct: 361 NSNLIKIGMEIVSMLNGVPLVIRNIGGLLKDNKSERFWLSFKDKELHQVLRQEQDALKEM 420
Query: 421 -------------AKLKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPN----DKDELI 480
+ LK YCALFPKDY+IQKDELIL WRAQGFIQPN D + L+
Sbjct: 421 QLILELSYKYLPSSNLKQCFRYCALFPKDYKIQKDELILLWRAQGFIQPNGNNDDDNYLV 480
Query: 481 DIGEDYFLELLSKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLK-NGIDKR 540
DIG+DYF++LLS+SFFQEV KD LG I CKMHDLMHDLACSI +ECV GLK N IDKR
Sbjct: 481 DIGDDYFMQLLSRSFFQEVEKDALGEIITCKMHDLMHDLACSIANHECVRGLKGNDIDKR 540
Query: 541 THHFSSTWMPRKDSEKLLQRLSKARNLRTFFMLNY---MDGWKLECDKFYHDHLRLRTLR 600
T HF R++ +L+ LSKA+ LRTFF ++ WK + + + RLR
Sbjct: 541 TRHFFLEGGSREN--QLMGHLSKAKYLRTFFRQDHYGVYSQWKNFKEIIFCNIFRLRAWH 600
Query: 601 LRLSPMDQSAKFLGSIIGTFKHLRYLSITSFNL--KNLPNCITELYNLETFILQYSSRLE 660
+L + + L I KHLRYL + + ++ +L +CI +LYNLETFI Q S L+
Sbjct: 601 SQLIGGIEDVESL-EFIAKLKHLRYLRMKNCSVISTDLLDCIMKLYNLETFIFQ-SRGLK 660
Query: 661 TLPSDINNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINL 720
+LP+ I NLINL+HLDL N L FLPDSITEL KLE LIL +CR LK LP+++KN NL
Sbjct: 661 SLPNTIGNLINLKHLDLSYNVNLGFLPDSITELCKLEALILRSCRDLKGLPKEIKNCYNL 720
Query: 721 RHLDLSWNKSIEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIALT 780
R LDLS N SIEF+PDSI QL NLE L+L+YC LK+LPEDTK L+ LKHLD+ G +A+T
Sbjct: 721 RRLDLSNNWSIEFIPDSIAQLINLETLILSYCRKLKKLPEDTKNLVKLKHLDLQGCLAVT 780
Query: 781 HMPKGLGQLTNLQTLNLFVLGDNNGGGLNELNELNKLKGRLSIEKLQFC-NVIDLQMKDK 840
HMPKGLG+LTNLQ +N FVLG+N GG L EL+ L KL+G+LSIE L+FC +++D QMK K
Sbjct: 781 HMPKGLGELTNLQIMNTFVLGNNKGGELKELDGLTKLRGKLSIEHLEFCTSIVDQQMKSK 840
Query: 841 LLQLKSCLQVLWLQWSRSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISGYKGVKLC 900
LQL LQ L L W + E + +YE VLDCLQPHSNL++I I GY GV LC
Sbjct: 841 FLQLMFGLQELKLHWEKHNGEL------EDVIYESVLDCLQPHSNLQKIYIDGYGGVNLC 900
BLAST of Lag0002715 vs. TAIR 10
Match:
AT3G14470.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 349.0 bits (894), Expect = 1.3e-95
Identity = 303/1116 (27.15%), Postives = 491/1116 (44.00%), Query Frame = 0
Query: 35 LDKLEDTLSAIQAVLLDAEERPINTNAMADWISKLKDVFYEIDDLLDEFSYETIRRQVMI 94
L++L L I AVL+DAEE+ I + W+++L+DV Y +D LD+ + E +R +
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIG- 98
Query: 95 RDGTSMEEVRTFFSKLKSHLKIDKKIKQIRDTLEAINHDKTQFSFSERVIDTRPDELRA- 154
+ +S +R ++ +D + + LE + + + ++ + EL A
Sbjct: 99 AESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLK--ELTAM 158
Query: 155 ----RRDIGSFIPEEEVIGRNDDKENVIHRLSNTNTTQEDIALISIVGMGGLGKTTLAQS 214
R S + E EV GR+DDK+ ++ L N I +++IVG+GG+GKTTL+Q
Sbjct: 159 IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQL 218
Query: 215 VYNHPNITKHFDLKLWVCVSEEFDVKIIIQKIIESATGKSPKSFLQMDSLQNELRRQIDG 274
+YN ++ +F K+W VSEEFDV I +K+ ES T + P F +D LQ +L+ ++ G
Sbjct: 219 LYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSR-PCEFTDLDVLQVKLKERLTG 278
Query: 275 K--KYLLVMDDVWNENHEKWVCLKRLLMGGAKGSRILITTRSERVAETFDTTFLYSLENL 334
+LLV+DD+WNEN W L++ + A+GS+IL+TTRS+RVA +++L+ L
Sbjct: 279 TGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQPL 338
Query: 335 NECNSWLLFKKMTGLEGQEKELENSDLIEIGKEIV------------------------- 394
++ + W LF K + G ++ N ++ ++ + IV
Sbjct: 339 SDGDCWSLFMKT--VFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIE 398
Query: 395 -------------------------------AKLKAMLFYCALFPKDYEIQKDELILQWR 454
A LK YC++FPK + +KD+++L W
Sbjct: 399 WERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWM 458
Query: 455 AQGFI-QPNDKDELIDIGEDYFLELLSKSFFQEVTKDELGNIKACKMHDLMHDLACSIT- 514
A+GF+ Q L ++G +YF EL S+S Q+ + MHD +++LA +
Sbjct: 459 AEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRYI-------MHDFINELAQFASG 518
Query: 515 --KNECVHGLKNGIDKRTHHFSSTWMPRKDSEKL-LQRLSKARNLRTFFMLNYMDGWKLE 574
++ G K + +RT + S ++ +E + + L + + LRTF L+ + +
Sbjct: 519 EFSSKFEDGCKLQVSERTRYLS--YLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSC 578
Query: 575 CDKFYHDHLRLRTLRLRLSPMDQSAKFLGSIIGTFKHLRYLSITSFNLKNLPNCITELYN 634
C +DQ ++ T LR LS++ + + LP
Sbjct: 579 C-------------------LDQMVS--EKLLPTLTRLRVLSLSHYKIARLP-------- 638
Query: 635 LETFILQYSSRLETLPSDINNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKL 694
P N+ + R LDL S TEL K
Sbjct: 639 ---------------PDFFKNISHARFLDL-----------SRTELEK------------ 698
Query: 695 KELPRDVKNWINLRHLDLSWNKSIEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLIN 754
LP S+ + NL+ LLL+YCS+LKELP D LIN
Sbjct: 699 --------------------------LPKSLCYMYNLQTLLLSYCSSLKELPTDISNLIN 758
Query: 755 LKHLDISGFIALTHMPKGLGQLTNLQTLNLFVLGDNNGGGLNELNELNKLKGRLSIEKLQ 814
L++LD+ G L MP+ G+L +LQTL F + ++G ++EL L+ L G+L I +LQ
Sbjct: 759 LRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQ 818
Query: 815 FCNVIDL-QMKDKLLQLKSCLQ---VLWLQWSRSFNEPTIGNLDNNNVYERVLDCLQPHS 874
V+D+ + L K L+ +W S S T + N V + L+PH
Sbjct: 819 --RVVDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEA--EVFEKLRPHR 878
Query: 875 NLREIRISGYKGVKLCNWLSSNFLGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPS 934
++ ++ I YKG + +WLS +V I L C LP Q P LK LH +
Sbjct: 879 HIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVG 938
Query: 935 IEYIGN-----------------------------------DDSVSSSTFFPSLEELKIV 994
++ IG D V+ FPSL++L I+
Sbjct: 939 LQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFIL 998
Query: 995 RMPKLKRWWKGTTSTESTPIKFPCLSQLEIRGCPLRILMSWH-----APLKSLTISDSEV 1036
R P+L T + P P L L I C L H L++L+I S
Sbjct: 999 RCPEL---------TGTLPTFLPSLISLHIYKCGLLDFQPDHHEYSYRNLQTLSIK-SSC 1029
BLAST of Lag0002715 vs. TAIR 10
Match:
AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 292.4 bits (747), Expect = 1.5e-78
Identity = 257/950 (27.05%), Postives = 420/950 (44.21%), Query Frame = 0
Query: 35 LDKLEDTLSAIQAVLLDAEERPINTNAMADWISKLKDVFYEIDDLLDEFSYETIRRQVMI 94
L +L+ L VL DA++R + + W++ +KD F++ +D+LDE E +RR+V+
Sbjct: 36 LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95
Query: 95 RDGTSMEEVRTFFSKLKS-HLKIDKKIKQIRDTLEAINHDKTQFSFSERVIDTRPDELRA 154
G + + ++ KI+ K++++ LE E P +A
Sbjct: 96 EAGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQA 155
Query: 155 RRDIGSFIPEEEVIGRNDDKENVIH-RLSNTNTTQEDIALISIVGMGGLGKTTLAQSVYN 214
R +P+ ++GR +DK +++ LS+ + A+IS+VGM G+GKTTL + V+N
Sbjct: 156 SRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFN 215
Query: 215 HPNITKHFDLKLWVCVSEEFDVKIIIQKIIESATGKSPKSFLQMDSLQNELRRQIDGKKY 274
+T+HF++K+W+ F+V + + +++ T S + + SLQ +L++ + GK++
Sbjct: 216 DYRVTEHFEVKMWISAGINFNVFTVTKAVLQDIT-SSAVNTEDLPSLQIQLKKTLSGKRF 275
Query: 275 LLVMDDVWNENHEKWVCLKRLLMGGAKGSRILITTRSERVAETFDTTFLYSLENLNECNS 334
LLV+DD W+E+ +W + +GS+I++TTRSE V+ +Y ++ +
Sbjct: 276 LLVLDDFWSESDSEWESFQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEEC 335
Query: 335 WLLFKKMT-----------GLEGQEKELEN----------------------SDLIEIGK 394
W L + LEG K + D + K
Sbjct: 336 WELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSK 395
Query: 395 ------------------EIVAKLKAMLFYCALFPKDYEIQKDELILQWRAQGFI-QPND 454
+ +LK C++FPK + ++EL+L W A + QP
Sbjct: 396 NFSSYTNSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRS 455
Query: 455 KDELIDIGEDYFLELLSKSFFQEVTKDELGNIKACKMHDLMHDLACSITKNECVHGLKNG 514
L DIG DY +L+++SFFQ + + + MHDLM+DLA +++ + C +
Sbjct: 456 SRRLEDIGNDYLGDLVAQSFFQRLDI----TMTSFVMHDLMNDLAKAVSGDFCFRLEDDN 515
Query: 515 IDK---RTHHFSSTWMPRKDSEKLLQRLSKARNLRTFFMLNYMDGWKLECDKFYHDHLRL 574
I + T HFS + + D+ + + A LRT N L
Sbjct: 516 IPEIPSTTRHFSFS-RSQCDASVAFRSICGAEFLRTILPFN--------------SPTSL 575
Query: 575 RTLRLRLSPMDQSAKFLGSIIGTFKHLRYLSITSFNLKNLPNCITELYNLETFILQYSSR 634
+L+L + K L ++ LR LS++ + + NLP
Sbjct: 576 ESLQL-------TEKVLNPLLNALSGLRILSLSHYQITNLPK------------------ 635
Query: 635 LETLPSDINNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWI 694
+L+ L L
Sbjct: 636 ------------SLKGLKL----------------------------------------- 695
Query: 695 NLRHLDLSWNKSIEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKLINLKHLDISGFIA 754
LR+LDLS K I+ LP+ + L NL+ LLL+ C +L LP+ +LINL+ LD+ G
Sbjct: 696 -LRYLDLSSTK-IKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVG-TP 755
Query: 755 LTHMPKGLGQLTNLQTLNLFVLGDNNGGGLNELNELNKLKGRLSIEKLQFCNV-IDLQMK 814
L MP G+ +L +LQ L+ FV+G +G GL+EL EL+ L+G L I +LQ NV + K
Sbjct: 756 LVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQ--NVAFASEAK 815
Query: 815 DKLLQLKSCLQVLWLQWSRSFNEPTIGNLDNNNVYER-VLDCLQPHSNLREIRISGYKGV 874
D L+ K L L L+W+ + G+ + ++ VL L+PH +L+ I Y+G
Sbjct: 816 DAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGG 875
Query: 875 KLCNWLSSNFLGCLVSIELSYCIRLEHLPQFDQFPHLKYLHFEDLPSIEYIGND----DS 922
WL + + S+ LS C LP Q P LKYL E ++ +G D ++
Sbjct: 876 AFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGEN 878
BLAST of Lag0002715 vs. TAIR 10
Match:
AT3G46730.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 198.0 bits (502), Expect = 3.8e-50
Identity = 209/827 (25.27%), Postives = 356/827 (43.05%), Query Frame = 0
Query: 9 VVANIITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTNAMADWISK 68
V ++ K+G + E+ L G+ D+L++L+ L+ I L D E R +W
Sbjct: 5 VTGFVLNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEAREREDEVSKEWTKL 64
Query: 69 LKDVFYEIDDLLDEFSY---ETIRRQVMIRDGTSMEEVRTFFSKLKSHLKIDKKIKQIRD 128
+ D+ Y+I+D+LD + E R+ ++R + + R ++ ++ + ++I I
Sbjct: 65 VLDIAYDIEDVLDTYFLKLEERSLRRGLLRLTNKIGKKRDAYNIVEDIRTLKRRILDITR 124
Query: 129 TLEAINHDKTQFSFSERVIDTRPDELRARRDIGSFIPEEEVIGRNDDKENVIHRLSNTNT 188
E E + + R +LR + EE V+G DD + ++ +L + N
Sbjct: 125 KRETFGIGSFNEPRGENITNVRVRQLRRAPPVDQ---EELVVGLEDDVKILLVKLLSDNE 184
Query: 189 TQEDIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKIIIQKIIESA 248
+ +ISI GMGGLGKT LA+ +YN ++ + FD + W VS+E+ + I+ +II S
Sbjct: 185 KDKSY-IISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILIRIIRSL 244
Query: 249 TGKSP------KSFLQMDSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMGGAK 308
S K F + + L+ L ++GK Y++V+DDVW+ + W LKR L +
Sbjct: 245 GIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVWDP--DAWESLKRALPCDHR 304
Query: 309 GSRILITTRSERVAETFD-TTFLYSLENLNECNSWLLF--KKMTGLEGQEKELENSDLIE 368
GS+++ITTR +AE + T + + L L SW LF K + +E +++L+ +
Sbjct: 305 GSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFERKAFSNIEKVDEDLQRT---- 364
Query: 369 IGKEIVAK---------------------------------------------------- 428
GKE+V K
Sbjct: 365 -GKEMVKKCGGLPLAIVVLSGLLSRKRTNEWHEVCASLWRRLKDNSIHISTVFDLSFKEM 424
Query: 429 ---LKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPNDKDELIDIGEDYFLELLSKSFF 488
LK Y ++FP+DYEI+ ++LI A+GFIQ +++ + D+ Y EL+ +S
Sbjct: 425 RHELKLCFLYFSVFPEDYEIKVEKLIHLLVAEGFIQEDEEMMMEDVARCYIDELVDRSLV 484
Query: 489 QEVTKDELGNIKACKMHDLMHDLACSITK--------NECVHGLKNGIDKRTHHFSSTWM 548
+ + E G + +C++HDL+ DLA K NE H + HH +
Sbjct: 485 -KAERIERGKVMSCRIHDLLRDLAIKKAKELNFVNVYNEKQHSSDICRREVVHHLMN--- 544
Query: 549 PRKDSEKLLQRLSKARNLRTFFMLNYMDGWKLECDKFYHDHLRLRTLRLRLSPMD----- 608
D +R++K +R+F + G+ Y + L+ LR+ M+
Sbjct: 545 ---DYYLCDRRVNK--RMRSFLFIGERRGFG------YVNTTNLKLKLLRVLNMEGLLFV 604
Query: 609 --QSAKFLGSIIGTFKHLRYLSITSFNLKNLPNCITELYNLETFILQYSSRLETLPSDIN 668
+ L +IG HLRYL I + LP I+ L L+T + + +D++
Sbjct: 605 SKNISNTLPDVIGELIHLRYLGIADTYVSILPASISNLRFLQTLDASGNDPFQ-YTTDLS 664
Query: 669 NLINLRHLD--------LCENDTLKFLPD-SITELHKLETLILHNCRKLKELPRDVKNWI 728
L +LRH+ + E L+ L S KL +L N + L+ D W+
Sbjct: 665 KLTSLRHVIGKFVGECLIGEGVNLQTLRSISSYSWSKLNHELLRNLQDLEIY--DHSKWV 724
Query: 729 NLRHLDLSWNKSIEFLPDSITQLNNLEILLLAYCSNLKELPEDTKKL----INLKHLDIS 741
+ R + L++ S ++ NL +L L N K E + +N L+
Sbjct: 725 DQRRVPLNF--------VSFSKPKNLRVLKLE-MRNFKLSSESRTTIGLVDVNFPSLESL 784
BLAST of Lag0002715 vs. TAIR 10
Match:
AT1G58848.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 196.1 bits (497), Expect = 1.4e-49
Identity = 270/1127 (23.96%), Postives = 464/1127 (41.17%), Query Frame = 0
Query: 14 ITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTNAMADWISKLKDVF 73
I L + +E + G++D++ +L+ L+ + + L DA+ + + + + + ++K++
Sbjct: 10 IQNLWNLLSQECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEII 69
Query: 74 YEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLKSHLKIDKKIKQIRDTLEAINHD 133
Y+ +D ++ F E Q + + + +R + + I + + + + D
Sbjct: 70 YDGEDTIETFVLE----QNLGKTSGIKKSIRRLACIIPDRRRYALGIGGLSNRISKVIRD 129
Query: 134 KTQFSFSERVID-----TRPDELRARRDIGSFIPEEEVIGRNDDKENVIHRLSNTNTTQE 193
F + ++D + D+ R R S + + +G + + ++ L +
Sbjct: 130 MQSFGVQQAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYL----VDEA 189
Query: 194 DIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKIIIQKIIESATGK 253
++ ++SI GMGGLGKTTLA+ V+NH ++ FD WVCVS++F + QKI+ K
Sbjct: 190 NVQVVSITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPK 249
Query: 254 -SPKSFLQM--DSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMGGAKGSRILI 313
K ++M D+LQ EL R ++ K L+V+DD+W + E W +K + KG ++L+
Sbjct: 250 EEEKKIMEMTQDTLQGELIRLLETSKSLIVLDDIWEK--EDWELIKP-IFPPTKGWKVLL 309
Query: 314 TTRSERVAETFDTTFL-YSLENLNECNSWLLFKKMT---------GLEGQEKEL------ 373
T+R+E VA +T+++ + E L +SW LF+++ ++ +++EL
Sbjct: 310 TSRNESVAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIK 369
Query: 374 -----------------------------ENSDLIEIG---------------------K 433
EN +G +
Sbjct: 370 HCGGLPLAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNYVLSLSFE 429
Query: 434 EIVAKLKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPNDKD-ELI-DIGEDYFLELLS 493
E+ + LK Y A FP DYEI L W A+G QP D E+I D+G+ Y EL+
Sbjct: 430 ELPSYLKHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIRDVGDVYIEELVR 489
Query: 494 KSFFQEVTKDELGNIKACKMHDLMHDLA---------CSITKNECVHGLKNGI---DKRT 553
++ + + C +HD+M ++ IT + G I +
Sbjct: 490 RNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSSRTSTGNSLSIVTSRRLV 549
Query: 554 HHFSSTWMPRKD-SEKLLQRLSKARNLRTFFMLNYMDGWKLECDKFYHDHLRLRTLR-LR 613
+ + T KD ++ L+ L N F+ GW +RL LR L
Sbjct: 550 YQYPITLDVEKDINDPKLRSLVVVANTYMFW-----GGWSWML--LGSSFIRLELLRVLD 609
Query: 614 LSPMDQSAKFLGSIIGTFKHLRYLSITSFNLKNLPNCITE---LYNLETFILQYSSRLET 673
+ L S IG HLRYL++ + ++P + L L IL S L
Sbjct: 610 IHRAKLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSGSTL-- 669
Query: 674 LPSDINNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLR 733
+P+ + + LR+L L D + ++ L KLETL + + D++ + LR
Sbjct: 670 VPNVLKEMQQLRYLAL-PKDMGRKTKLELSNLVKLETLKNFSTKNCS--LEDLRGMVRLR 729
Query: 734 HLDLSWNK--SIEFLPDSITQLNNLEILLLAYCSNLKELPED--TKKLINLKHLDISGFI 793
L + K S+E L SI L LE L + + E + LK L + +
Sbjct: 730 TLTIELRKETSLETLAASIGGLKYLESLTITDLGSEMRTKEAGIVFDFVYLKTLTLK--L 789
Query: 794 ALTHMPKGLGQLTNLQTLNL--FVLGDNNGGGLNELNELNKLKGRLS--IEKLQFCNVID 853
+ + K ++L TL L L ++ L +L++L +L+ R K C+
Sbjct: 790 YMPRLSKEQHFPSHLTTLYLQHCRLEEDPMPILEKLHQLKELELRRKSFSGKEMVCSSGG 849
Query: 854 LQMKDKLLQLKSCLQVLWLQWS-RSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISG 913
K L +K + W W + P + LD + DC
Sbjct: 850 FPQLQK-LSIKGLEE--WEDWKVEESSMPVLHTLD-------IRDCR------------- 909
Query: 914 YKGVKLCNWLSSNFLGCLVSIELSY-CIRLEHLPQFDQFPHLKYLHFEDLPSIEYIGNDD 973
KL + L SI L + C+ + +P ++ HLK L
Sbjct: 910 ----KLKQLPDEHLPSHLTSISLFFCCLEEDPMPTLERLVHLKELQL----LFRSFSGRI 969
Query: 974 SVSSSTFFPSLEELKIVRMPKLKRWWKGTTSTESTPIKFPCLSQLEIRGCP-LRILMSWH 1033
V + + FP L +LK+ + L+ W S P L LEIR CP L+ L +
Sbjct: 970 MVCAGSGFPQLHKLKLSELDGLEEWIVEDGS-------MPQLHTLEIRRCPKLKKLPNGF 1029
Query: 1034 APLKSLTISDSEVNEFDVVPWQNFENLSSLILFNFSKLEYLPKCLQHVTTLQNLRLWDCE 1037
L++L E+NE + W+ + I+ + S + L LR+W+C
Sbjct: 1030 PQLQNL-----ELNELE--EWEEW------IVEDGS-----------MPLLHTLRIWNCP 1046
BLAST of Lag0002715 vs. TAIR 10
Match:
AT1G59218.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 196.1 bits (497), Expect = 1.4e-49
Identity = 270/1127 (23.96%), Postives = 464/1127 (41.17%), Query Frame = 0
Query: 14 ITKLGSQAVREIPMLWGLDDELDKLEDTLSAIQAVLLDAEERPINTNAMADWISKLKDVF 73
I L + +E + G++D++ +L+ L+ + + L DA+ + + + + + ++K++
Sbjct: 10 IQNLWNLLSQECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEII 69
Query: 74 YEIDDLLDEFSYETIRRQVMIRDGTSMEEVRTFFSKLKSHLKIDKKIKQIRDTLEAINHD 133
Y+ +D ++ F E Q + + + +R + + I + + + + D
Sbjct: 70 YDGEDTIETFVLE----QNLGKTSGIKKSIRRLACIIPDRRRYALGIGGLSNRISKVIRD 129
Query: 134 KTQFSFSERVID-----TRPDELRARRDIGSFIPEEEVIGRNDDKENVIHRLSNTNTTQE 193
F + ++D + D+ R R S + + +G + + ++ L +
Sbjct: 130 MQSFGVQQAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYL----VDEA 189
Query: 194 DIALISIVGMGGLGKTTLAQSVYNHPNITKHFDLKLWVCVSEEFDVKIIIQKIIESATGK 253
++ ++SI GMGGLGKTTLA+ V+NH ++ FD WVCVS++F + QKI+ K
Sbjct: 190 NVQVVSITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPK 249
Query: 254 -SPKSFLQM--DSLQNELRRQIDGKKYLLVMDDVWNENHEKWVCLKRLLMGGAKGSRILI 313
K ++M D+LQ EL R ++ K L+V+DD+W + E W +K + KG ++L+
Sbjct: 250 EEEKKIMEMTQDTLQGELIRLLETSKSLIVLDDIWEK--EDWELIKP-IFPPTKGWKVLL 309
Query: 314 TTRSERVAETFDTTFL-YSLENLNECNSWLLFKKMT---------GLEGQEKEL------ 373
T+R+E VA +T+++ + E L +SW LF+++ ++ +++EL
Sbjct: 310 TSRNESVAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIK 369
Query: 374 -----------------------------ENSDLIEIG---------------------K 433
EN +G +
Sbjct: 370 HCGGLPLAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNYVLSLSFE 429
Query: 434 EIVAKLKAMLFYCALFPKDYEIQKDELILQWRAQGFIQPNDKD-ELI-DIGEDYFLELLS 493
E+ + LK Y A FP DYEI L W A+G QP D E+I D+G+ Y EL+
Sbjct: 430 ELPSYLKHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIRDVGDVYIEELVR 489
Query: 494 KSFFQEVTKDELGNIKACKMHDLMHDLA---------CSITKNECVHGLKNGI---DKRT 553
++ + + C +HD+M ++ IT + G I +
Sbjct: 490 RNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSSRTSTGNSLSIVTSRRLV 549
Query: 554 HHFSSTWMPRKD-SEKLLQRLSKARNLRTFFMLNYMDGWKLECDKFYHDHLRLRTLR-LR 613
+ + T KD ++ L+ L N F+ GW +RL LR L
Sbjct: 550 YQYPITLDVEKDINDPKLRSLVVVANTYMFW-----GGWSWML--LGSSFIRLELLRVLD 609
Query: 614 LSPMDQSAKFLGSIIGTFKHLRYLSITSFNLKNLPNCITE---LYNLETFILQYSSRLET 673
+ L S IG HLRYL++ + ++P + L L IL S L
Sbjct: 610 IHRAKLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSGSTL-- 669
Query: 674 LPSDINNLINLRHLDLCENDTLKFLPDSITELHKLETLILHNCRKLKELPRDVKNWINLR 733
+P+ + + LR+L L D + ++ L KLETL + + D++ + LR
Sbjct: 670 VPNVLKEMQQLRYLAL-PKDMGRKTKLELSNLVKLETLKNFSTKNCS--LEDLRGMVRLR 729
Query: 734 HLDLSWNK--SIEFLPDSITQLNNLEILLLAYCSNLKELPED--TKKLINLKHLDISGFI 793
L + K S+E L SI L LE L + + E + LK L + +
Sbjct: 730 TLTIELRKETSLETLAASIGGLKYLESLTITDLGSEMRTKEAGIVFDFVYLKTLTLK--L 789
Query: 794 ALTHMPKGLGQLTNLQTLNL--FVLGDNNGGGLNELNELNKLKGRLS--IEKLQFCNVID 853
+ + K ++L TL L L ++ L +L++L +L+ R K C+
Sbjct: 790 YMPRLSKEQHFPSHLTTLYLQHCRLEEDPMPILEKLHQLKELELRRKSFSGKEMVCSSGG 849
Query: 854 LQMKDKLLQLKSCLQVLWLQWS-RSFNEPTIGNLDNNNVYERVLDCLQPHSNLREIRISG 913
K L +K + W W + P + LD + DC
Sbjct: 850 FPQLQK-LSIKGLEE--WEDWKVEESSMPVLHTLD-------IRDCR------------- 909
Query: 914 YKGVKLCNWLSSNFLGCLVSIELSY-CIRLEHLPQFDQFPHLKYLHFEDLPSIEYIGNDD 973
KL + L SI L + C+ + +P ++ HLK L
Sbjct: 910 ----KLKQLPDEHLPSHLTSISLFFCCLEEDPMPTLERLVHLKELQL----LFRSFSGRI 969
Query: 974 SVSSSTFFPSLEELKIVRMPKLKRWWKGTTSTESTPIKFPCLSQLEIRGCP-LRILMSWH 1033
V + + FP L +LK+ + L+ W S P L LEIR CP L+ L +
Sbjct: 970 MVCAGSGFPQLHKLKLSELDGLEEWIVEDGS-------MPQLHTLEIRRCPKLKKLPNGF 1029
Query: 1034 APLKSLTISDSEVNEFDVVPWQNFENLSSLILFNFSKLEYLPKCLQHVTTLQNLRLWDCE 1037
L++L E+NE + W+ + I+ + S + L LR+W+C
Sbjct: 1030 PQLQNL-----ELNELE--EWEEW------IVEDGS-----------MPLLHTLRIWNCP 1046
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022154678.1 | 0.0e+00 | 53.80 | putative disease resistance protein RGA4 [Momordica charantia] | [more] |
| XP_016902648.1 | 4.0e-309 | 54.25 | PREDICTED: putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis ... | [more] |
| XP_022154711.1 | 4.3e-306 | 55.91 | LOW QUALITY PROTEIN: disease resistance protein RGA2-like [Momordica charantia] | [more] |
| XP_022152874.1 | 1.8e-296 | 49.52 | disease resistance protein RGA2-like [Momordica charantia] | [more] |
| XP_031736581.1 | 1.0e-291 | 52.45 | putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651704.1 hypothe... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q7XBQ9 | 7.6e-120 | 30.27 | Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... | [more] |
| Q7XA40 | 3.2e-118 | 30.91 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XA39 | 6.0e-117 | 29.63 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XA42 | 7.8e-117 | 29.75 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q9LRR4 | 1.9e-94 | 27.15 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DKY9 | 0.0e+00 | 53.80 | putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
| A0A1S4E349 | 1.9e-309 | 54.25 | putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo OX=3... | [more] |
| A0A6J1DPI9 | 2.1e-306 | 55.91 | LOW QUALITY PROTEIN: disease resistance protein RGA2-like OS=Momordica charantia... | [more] |
| A0A6J1DHD4 | 8.8e-297 | 49.52 | disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020... | [more] |
| A0A5A7UTU8 | 1.1e-286 | 57.01 | Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var.... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G14470.1 | 1.3e-95 | 27.15 | NB-ARC domain-containing disease resistance protein | [more] |
| AT3G14460.1 | 1.5e-78 | 27.05 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
| AT3G46730.1 | 3.8e-50 | 25.27 | NB-ARC domain-containing disease resistance protein | [more] |
| AT1G58848.1 | 1.4e-49 | 23.96 | Disease resistance protein (CC-NBS-LRR class) family | [more] |
| AT1G59218.1 | 1.4e-49 | 23.96 | Disease resistance protein (CC-NBS-LRR class) family | [more] |
