Lag0002699 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002699
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr4: 44928191 .. 44933016 (+)
RNA-Seq ExpressionLag0002699
SyntenyLag0002699
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGATAGCTGTGCGGTTTGTGCCGATACTCTTGAATGGGTTGCCTATGGGTCTTGTGGCCATCGCGATGTCTGCTCCACCTGCGTTGCTCGTCTTCGATTCATTTGCGGCGATCGCCGCTGTTGCATCTGCAAGTCGGAATCCGCCGTCGTGTTCGTCACTAAGGTGAGGCTGCAGCTATTATCTGTTCTGCGATCGCATTCAATCTTTGGGCGTCTTTGATTATTGAATTCTGAATTTGTTTTAATTTCATGTGCAATTTGGTTATCTCTCTAGTGTTCTGCTGCTGTAGTTGAATTCGAATTCTTCATAGAGCAATTTTTTTTTCACTTTAATTTATTGCATTTCTGAAATTATGAATATGTTTTTGCTGCTGTTTGATTTGGATTGTCATGCTTACACCAAGGGAATGTCGTATGCAATTTGAACGTCTGATTATTCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTCTCTGTGCTTCAATATTGATGTAGTATTGTTAGAGTTCATTTCTTATTGTCTTTCTTGGATAGATAAAGGGCTGTTTGGAAATTGCTTTAGAAACAGCTTTGCATTTTGTTAGTACAAAATAAGAATCATGTATCTGGGAACAGTGGTTTTGATGTGAATAATAATATTACCTCGTAACACAATCATAATTTTCAAAAACAATTTTGAAAATGGAAACAGAAAACTGTTATCAGAGCAGTTAGCCAATGACTAGTGGTGGCAACTGTACATCAATAAGGTCGAGGAAAGGAGAGTTTATGTACTTAGCTTGATCTCTCATTAGCAATCATATTTTATTATCATCTTTATATGCTTATAGGAATATTGGTATCCATAACATTGCAGAAGTTAAAAGAAAGAGGGGGAAAAAAGAAAAACACTGCAAAAGTTTTCCTAATTACAATCACAATGATTGGTTACAGGCTCAGTGTTACCTTTTTTCTTATTTTCAGTGATTAATACTTTCAGCTGACTAGAGTAAATTAGGATTAAGGAAACTAAAGTAAGCCACCCTCAGTGAACTAAATAAATTCCCAAGTCTCTTTCAAATTGAAGGAAAATACTTCTAGATCTCAATGCAACCAATTCTATATGGCTGCTAAGAAGCACAATTAAAAAAAAAAAAAAGTGATCCGGCATACCTTGGTATCTCCATTGTCCATATTTGTTATAGTCCTTCTTGATACTCCAAAGCTTCCATACCTCATTCCATATTATTCTGCTCCTAAAATTGTTGGTCTTGGCTGTTTGACATGTACACCAATGATCAAAGTTCATGTTTGATTTTAAAAATAAAGAGACAAGTGAGAACTCAGTTGTTATCTGAAATTTATAATTAGGTGGATTTGGCGCTTATTCAAACATTATTTTGATTGAGATGGAGGACTGTTTTGTTCTTTCATTGTGCTTATGTTGTTCATGTCTGGTTCTGCGATAGGCCTTAGGAGATTATACAAGGACAATCAATGACTTCGCAGTCTTTCCTTCTGAACCAAGAGAAGGTCGTAATGGATCCTATTGGTATCATGAAGACACACAGACATTTTTTGATGATGTTGACCACTACAAGATGATAAAAGCAATGTGCAGGTTGTCATGCAGTGTGTGTGATAAGATAGGTGAAGATCAACCAAATGATGGCTCTAAGCGCCGAGGAAGATTTCGGAACATTGAGCAGCTAAAGGGCCATTTGTTTCATCGCCATAAGTTGTTTATGTGCAGCCTTTGTTTGGAAGGAAGAAAGGTATGCTTATTATTCTTTTTTGAGATACTTGTGGTAGGTTGATAGTTATTATGACAATTATTATATGTGAACATTTTTTTTTCTTGAAAAGAAAAAAAGAAAGGTATGTGAACATGAACCAGAGTTTATCTGGTCTCTTCTTTGATTGCAACCATAGTGCTGACTAGCTTTTCTGGTGTGTCTCAGGTTTTTATTTGTGAACAGAAGCTATATAATAGAGCACAATTGAATCAGCATATACACACTGGTGACTCCGAGGTGGATGGAAGTGAGAGTGAGAGAGGTGGTTTCACTGGGCATCCTATGTGTGAATTTTGCAGAACTCCTTTTTACGGAGATAATGAGCTGTATACTCATATGTCGACTGAGCATTATACATGTCATATATGTCAAAGGTAAACTAAACATGTTTGATTTGTGACTAGGGTGTATAAATTTACACCAAACTGGCATTCCAATGTAACTGAACTGTACGAAATTGATTTCATTTTGTTTCTTACTTGAATAACCCTCAATCTATTTTCTTAATAGACTTAATTAACTGCCCATTTAATCAGAATTCAATATCTTTTAGAAGGATTCATTGGTTAACATTAATCTGTATATATATATTCGTAACTAAACTTTCCATAAACTCCATTTCTGTCAGCAAAATGAAAATAGGTGGGTTGGGATTATTTTTCTCTTGGAAAAAAAAAGGAAAGAAGAAACGGATGCAAAGAACTGAACCAATCAGTTCTATTTTTGTTCCATAATTCTTCTTCAGGGAGTCAAACCTTTCAATAATCTAGGTCAAAAATCGAATTGTACTGAACTGATTCCTCCTCTACTTCATCATCCTTCATCATTGCTAACTTGGTGAGATTAAAAATTGTTTTCTTTTTTGGGTTGCAGGCTCCATCCTGGGCAGTATGAATACTATAAGAATTATGACGACCTAGAGGCAAGTTGTGATTACTATACCCCCTTTAACAATGAATATTGTCTTTTTAATCACTGTTTTGAGGTGTGAATGTATCTGAAGATAGTGAACTTTTTGGCCACAGATCCATTTCCGACAAGGACATTTTCTTTGTGAAGATGAAGCCTGCCTTGCTAAAAAGTTTATAGTGTTCCAATCTGAAGCAGAAATGAAGGTAACTTAGAACATCCAGTAAGCGTTGTGCATGTGTGTATTTAGTTAAAGCTCTCTGTTTTGTTTTTGCCCATTTCTTTTAAGTTTGTAAGGCTGAGGCTTGCTCCATCGTAGTGCATTGTTTCTTGATGTCAATTTGTTATTTTTCAGAGGCATAATACTATTGAGCATGGTGGCAAGCTATCTCGATCAAAGCGAAATGCTGCTTTGCAGGTATGTATGATTGCTCTCCCAATTTTGGTCTTTAAGAAATGCAAAATTTTATTTTATTTTAAATCTTAATTCCCTTTCCTGCATTATTTCTCAATTAGCGTTGTCTTTTCTAGATACCAACCAGTTTTCGATATCGAAGAAGTAACGAGCAGGATAATCGCCGTGGTAGACGGACATTTCGTCGTGATTCTTCTGACGATCTACTTTCTTTAGCCATTCAAGAAAGTTTTGAATCAGCCAATGTTGATGACACCAATCATGACTCATTACCTGGCGGACAAGTAGCCTCGGATCAGGGAAATCTAAGCAACATTGATCCTTTAGTTGAACCATTTGAATCATTAGCAACAACAGATGCCGAATCAGCTTCTAGATACCTTCAAGCCTTGGGCCATTCTAGGAATTCCCAATTGGGGCAATCGTCCTTTCCACCCCTTTCCACAGCACCTAGCAGCAGCCATCCAAAGCCCATGCAGGACAAAGACGCTATACATAATAACAGTATGGCAGCTCATCTTCGCCGCCAGAGGAATGTGACTGTTCTGAATTCTGCTCAAGGTTGGCCAAAATCAAGTCGTGCCCCTGTGCCACCATCAAATAATTCATCTCAAGCTTGGCCTGCAATAAATGTTAATCATCCAGCGTCAAGCAGTTCTGGGCAGACTAAGAGTGTTGTCACCATCAATAATGGCTCTTCAGTCTCTGGTTACTCAAATGTTGCTCAGGTGCATGCGAAGGCTCGATCTAGTTCTTCAAGTGGATCAGGTAGTAGCAGTAGGATCAGTCACTCTGCTTCCGCTCCCAGTCTCACCGACATGGCCTATACTGAACCCTCAGTTAATGAATTCCCTCCAGTTTCCGCAGCACAGGCTCGGAAGGTGCCCTCAAGTAGCCAGAGTTCAATGAACATGGAAGATGTCCAGACAGCAAACAAATCTCTAGTTGAAAAGATACGCGCTGCTCTTGATTTTGACCCAGATAGATATGCCATTTTTAAAGATATATCTGCACAATATCGTCAGGGTCAGATTGACACGGAAATGTATCTAGACTGTGTGCAGCAGTTCGGTCTGTCACATCTTGTGCTTGAGCTGGCTAGGCTCTGTCCTGATACTCAGAAACAGAAAGAACTTGTGGAGACCTATAAAGCTAGTTTACATAAAGATGTGGTCCAAGAAAATGGTAGGGCTCAAGATAGTATTCAATTTAAAGACAGAAGTAAAGGAAAGAAAGGTAAAGGGAAGTCTATGGATGTTGTTAAGGACAGCACATCCAAGGACAAATTTGCCGATAGTATCTTGAGTAGTGTTCGGGAACTGCAGTCAAGTTTGAGGCCATCGGAGGAGAATGTAGAGGTATTGTCAAAGGGTGACTACCGTGCTGCGAAAGGGAAATCGAAGTTATCATCTGACGACCAACAAGGTGGGATAGGAGGCCAAAACAGTCAACCGTCTACTGGTTTATCGAACCAAAGCACCGGGGATGGGGGTGGGGGGAGCAAGCAGAAAAAGAAAACTTCGAAGTTCCATAGAGTTCGTTTAGGTGATGGTTCAGTTGCTGCACTTCTAGATTTAAAGAACTCCAATCCCGAACCTGACCCGGTAGAAAGAGTAGAGGACAGGGACAATGCTGGGGCATTGCCTGTTCGGGGCGTGTGGAGAAATGGTGCTCAGAAGCTTTTCTCCTAA

mRNA sequence

ATGGACGATAGCTGTGCGGTTTGTGCCGATACTCTTGAATGGGTTGCCTATGGGTCTTGTGGCCATCGCGATGTCTGCTCCACCTGCGTTGCTCGTCTTCGATTCATTTGCGGCGATCGCCGCTGTTGCATCTGCAAGTCGGAATCCGCCGTCGTGTTCGTCACTAAGGCCTTAGGAGATTATACAAGGACAATCAATGACTTCGCAGTCTTTCCTTCTGAACCAAGAGAAGGTCGTAATGGATCCTATTGGTATCATGAAGACACACAGACATTTTTTGATGATGTTGACCACTACAAGATGATAAAAGCAATGTGCAGGTTGTCATGCAGTGTGTGTGATAAGATAGGTGAAGATCAACCAAATGATGGCTCTAAGCGCCGAGGAAGATTTCGGAACATTGAGCAGCTAAAGGGCCATTTGTTTCATCGCCATAAGTTGTTTATGTGCAGCCTTTGTTTGGAAGGAAGAAAGGTTTTTATTTGTGAACAGAAGCTATATAATAGAGCACAATTGAATCAGCATATACACACTGGTGACTCCGAGGTGGATGGAAGTGAGAGTGAGAGAGGTGGTTTCACTGGGCATCCTATGTGTGAATTTTGCAGAACTCCTTTTTACGGAGATAATGAGCTGTATACTCATATGTCGACTGAGCATTATACATGTCATATATGTCAAAGGCTCCATCCTGGGCAGTATGAATACTATAAGAATTATGACGACCTAGAGATCCATTTCCGACAAGGACATTTTCTTTGTGAAGATGAAGCCTGCCTTGCTAAAAAGTTTATAGTGTTCCAATCTGAAGCAGAAATGAAGAGGCATAATACTATTGAGCATGGTGGCAAGCTATCTCGATCAAAGCGAAATGCTGCTTTGCAGATACCAACCAGTTTTCGATATCGAAGAAGTAACGAGCAGGATAATCGCCGTGGTAGACGGACATTTCGTCGTGATTCTTCTGACGATCTACTTTCTTTAGCCATTCAAGAAAGTTTTGAATCAGCCAATGTTGATGACACCAATCATGACTCATTACCTGGCGGACAAGTAGCCTCGGATCAGGGAAATCTAAGCAACATTGATCCTTTAGTTGAACCATTTGAATCATTAGCAACAACAGATGCCGAATCAGCTTCTAGATACCTTCAAGCCTTGGGCCATTCTAGGAATTCCCAATTGGGGCAATCGTCCTTTCCACCCCTTTCCACAGCACCTAGCAGCAGCCATCCAAAGCCCATGCAGGACAAAGACGCTATACATAATAACAGTATGGCAGCTCATCTTCGCCGCCAGAGGAATGTGACTGTTCTGAATTCTGCTCAAGGTTGGCCAAAATCAAGTCGTGCCCCTGTGCCACCATCAAATAATTCATCTCAAGCTTGGCCTGCAATAAATGTTAATCATCCAGCGTCAAGCAGTTCTGGGCAGACTAAGAGTGTTGTCACCATCAATAATGGCTCTTCAGTCTCTGGTTACTCAAATGTTGCTCAGGTGCATGCGAAGGCTCGATCTAGTTCTTCAAGTGGATCAGGTAGTAGCAGTAGGATCAGTCACTCTGCTTCCGCTCCCAGTCTCACCGACATGGCCTATACTGAACCCTCAGTTAATGAATTCCCTCCAGTTTCCGCAGCACAGGCTCGGAAGGTGCCCTCAAGTAGCCAGAGTTCAATGAACATGGAAGATGTCCAGACAGCAAACAAATCTCTAGTTGAAAAGATACGCGCTGCTCTTGATTTTGACCCAGATAGATATGCCATTTTTAAAGATATATCTGCACAATATCGTCAGGGTCAGATTGACACGGAAATGTATCTAGACTGTGTGCAGCAGTTCGGTCTGTCACATCTTGTGCTTGAGCTGGCTAGGCTCTGTCCTGATACTCAGAAACAGAAAGAACTTGTGGAGACCTATAAAGCTAGTTTACATAAAGATGTGGTCCAAGAAAATGGTAGGGCTCAAGATAGTATTCAATTTAAAGACAGAAGTAAAGGAAAGAAAGGTAAAGGGAAGTCTATGGATGTTGTTAAGGACAGCACATCCAAGGACAAATTTGCCGATAGTATCTTGAGTAGTGTTCGGGAACTGCAGTCAAGTTTGAGGCCATCGGAGGAGAATGTAGAGGTATTGTCAAAGGGTGACTACCGTGCTGCGAAAGGGAAATCGAAGTTATCATCTGACGACCAACAAGGTGGGATAGGAGGCCAAAACAGTCAACCGTCTACTGGTTTATCGAACCAAAGCACCGGGGATGGGGGTGGGGGGAGCAAGCAGAAAAAGAAAACTTCGAAGTTCCATAGAGTTCGTTTAGGTGATGGTTCAGTTGCTGCACTTCTAGATTTAAAGAACTCCAATCCCGAACCTGACCCGGTAGAAAGAGTAGAGGACAGGGACAATGCTGGGGCATTGCCTGTTCGGGGCGTGTGGAGAAATGGTGCTCAGAAGCTTTTCTCCTAA

Coding sequence (CDS)

ATGGACGATAGCTGTGCGGTTTGTGCCGATACTCTTGAATGGGTTGCCTATGGGTCTTGTGGCCATCGCGATGTCTGCTCCACCTGCGTTGCTCGTCTTCGATTCATTTGCGGCGATCGCCGCTGTTGCATCTGCAAGTCGGAATCCGCCGTCGTGTTCGTCACTAAGGCCTTAGGAGATTATACAAGGACAATCAATGACTTCGCAGTCTTTCCTTCTGAACCAAGAGAAGGTCGTAATGGATCCTATTGGTATCATGAAGACACACAGACATTTTTTGATGATGTTGACCACTACAAGATGATAAAAGCAATGTGCAGGTTGTCATGCAGTGTGTGTGATAAGATAGGTGAAGATCAACCAAATGATGGCTCTAAGCGCCGAGGAAGATTTCGGAACATTGAGCAGCTAAAGGGCCATTTGTTTCATCGCCATAAGTTGTTTATGTGCAGCCTTTGTTTGGAAGGAAGAAAGGTTTTTATTTGTGAACAGAAGCTATATAATAGAGCACAATTGAATCAGCATATACACACTGGTGACTCCGAGGTGGATGGAAGTGAGAGTGAGAGAGGTGGTTTCACTGGGCATCCTATGTGTGAATTTTGCAGAACTCCTTTTTACGGAGATAATGAGCTGTATACTCATATGTCGACTGAGCATTATACATGTCATATATGTCAAAGGCTCCATCCTGGGCAGTATGAATACTATAAGAATTATGACGACCTAGAGATCCATTTCCGACAAGGACATTTTCTTTGTGAAGATGAAGCCTGCCTTGCTAAAAAGTTTATAGTGTTCCAATCTGAAGCAGAAATGAAGAGGCATAATACTATTGAGCATGGTGGCAAGCTATCTCGATCAAAGCGAAATGCTGCTTTGCAGATACCAACCAGTTTTCGATATCGAAGAAGTAACGAGCAGGATAATCGCCGTGGTAGACGGACATTTCGTCGTGATTCTTCTGACGATCTACTTTCTTTAGCCATTCAAGAAAGTTTTGAATCAGCCAATGTTGATGACACCAATCATGACTCATTACCTGGCGGACAAGTAGCCTCGGATCAGGGAAATCTAAGCAACATTGATCCTTTAGTTGAACCATTTGAATCATTAGCAACAACAGATGCCGAATCAGCTTCTAGATACCTTCAAGCCTTGGGCCATTCTAGGAATTCCCAATTGGGGCAATCGTCCTTTCCACCCCTTTCCACAGCACCTAGCAGCAGCCATCCAAAGCCCATGCAGGACAAAGACGCTATACATAATAACAGTATGGCAGCTCATCTTCGCCGCCAGAGGAATGTGACTGTTCTGAATTCTGCTCAAGGTTGGCCAAAATCAAGTCGTGCCCCTGTGCCACCATCAAATAATTCATCTCAAGCTTGGCCTGCAATAAATGTTAATCATCCAGCGTCAAGCAGTTCTGGGCAGACTAAGAGTGTTGTCACCATCAATAATGGCTCTTCAGTCTCTGGTTACTCAAATGTTGCTCAGGTGCATGCGAAGGCTCGATCTAGTTCTTCAAGTGGATCAGGTAGTAGCAGTAGGATCAGTCACTCTGCTTCCGCTCCCAGTCTCACCGACATGGCCTATACTGAACCCTCAGTTAATGAATTCCCTCCAGTTTCCGCAGCACAGGCTCGGAAGGTGCCCTCAAGTAGCCAGAGTTCAATGAACATGGAAGATGTCCAGACAGCAAACAAATCTCTAGTTGAAAAGATACGCGCTGCTCTTGATTTTGACCCAGATAGATATGCCATTTTTAAAGATATATCTGCACAATATCGTCAGGGTCAGATTGACACGGAAATGTATCTAGACTGTGTGCAGCAGTTCGGTCTGTCACATCTTGTGCTTGAGCTGGCTAGGCTCTGTCCTGATACTCAGAAACAGAAAGAACTTGTGGAGACCTATAAAGCTAGTTTACATAAAGATGTGGTCCAAGAAAATGGTAGGGCTCAAGATAGTATTCAATTTAAAGACAGAAGTAAAGGAAAGAAAGGTAAAGGGAAGTCTATGGATGTTGTTAAGGACAGCACATCCAAGGACAAATTTGCCGATAGTATCTTGAGTAGTGTTCGGGAACTGCAGTCAAGTTTGAGGCCATCGGAGGAGAATGTAGAGGTATTGTCAAAGGGTGACTACCGTGCTGCGAAAGGGAAATCGAAGTTATCATCTGACGACCAACAAGGTGGGATAGGAGGCCAAAACAGTCAACCGTCTACTGGTTTATCGAACCAAAGCACCGGGGATGGGGGTGGGGGGAGCAAGCAGAAAAAGAAAACTTCGAAGTTCCATAGAGTTCGTTTAGGTGATGGTTCAGTTGCTGCACTTCTAGATTTAAAGAACTCCAATCCCGAACCTGACCCGGTAGAAAGAGTAGAGGACAGGGACAATGCTGGGGCATTGCCTGTTCGGGGCGTGTGGAGAAATGGTGCTCAGAAGCTTTTCTCCTAA

Protein sequence

MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGDYTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQPNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGDSEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNYDDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSFRYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNLSNIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKDAIHNNSMAAHLRRQRNVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHPASSSSGQTKSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVNEFPPVSAAQARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQGQIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSIQFKDRSKGKKGKGKSMDVVKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRAAKGKSKLSSDDQQGGIGGQNSQPSTGLSNQSTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNPEPDPVERVEDRDNAGALPVRGVWRNGAQKLFS
Homology
BLAST of Lag0002699 vs. NCBI nr
Match: XP_038897973.1 (E3 ubiquitin-protein ligase HEL2 [Benincasa hispida])

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 753/825 (91.27%), Postives = 777/825 (94.18%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEPREGR+GSYWYHEDTQ FFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRSGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PND SKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHP+CEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPICEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNLS 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFE+ANVDDTNHD LP GQ ASDQ N+S
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFETANVDDTNHDPLPSGQGASDQENIS 360

Query: 361 NIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKDA 420
           NIDPL+EPFE+LATTD ESASRYLQALGHSRNSQL QSSFPPLSTA SSSHPKP QDKDA
Sbjct: 361 NIDPLIEPFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPSQDKDA 420

Query: 421 IHNNSMAAHLRRQR-NVTVLNSAQGWPKSSRAPV-PPSNNSSQAWPAINVNHPASSSSGQ 480
           IHN+SMAAHLRRQR NVTVLNSA GWPKSSRAPV PPSNNSSQAWPAIN NH ASSSS Q
Sbjct: 421 IHNDSMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPPSNNSSQAWPAINANHAASSSSRQ 480

Query: 481 TKSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVN 540
           TK VVTINNG SVS YSN AQ+HAKARSSSSSGSGSSSRISHSASAPSLTD+AYTEPSVN
Sbjct: 481 TKGVVTINNGPSVSAYSNAAQMHAKARSSSSSGSGSSSRISHSASAPSLTDIAYTEPSVN 540

Query: 541 EFPPVSAAQARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQ 600
           EFPPVS  QA K PSSSQSSMNMEDVQTANKSLVEKIRAALDFD DRYAIFKDISAQYRQ
Sbjct: 541 EFPPVSTVQAWKAPSSSQSSMNMEDVQTANKSLVEKIRAALDFDQDRYAIFKDISAQYRQ 600

Query: 601 GQIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSI 660
           GQIDTEMYLDCVQQFGLSHL+LELARLCPD QKQKELVETY AS HKDV+  NGRAQDSI
Sbjct: 601 GQIDTEMYLDCVQQFGLSHLLLELARLCPDPQKQKELVETYNASFHKDVIPVNGRAQDSI 660

Query: 661 QFKDRSKGKKGKGKSMDVVKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRA 720
           Q KD SKGKKGKGKS++ VKDS+SKDK ADSILSSVRELQSS RP EE+VEVLSKG+YRA
Sbjct: 661 QIKDNSKGKKGKGKSIE-VKDSSSKDKLADSILSSVRELQSSFRPLEEDVEVLSKGEYRA 720

Query: 721 AKGKSKLSSDDQQGGIGGQNSQPSTGLSNQSTGD-GGGGSKQKKKTSKFHRVRLGDGSVA 780
           +KGK K+SSD+QQGG GGQNSQ STGL N+STGD GGGGSKQKKKTSKFHRVRLGDGSVA
Sbjct: 721 SKGKLKISSDNQQGGTGGQNSQLSTGLLNESTGDGGGGGSKQKKKTSKFHRVRLGDGSVA 780

Query: 781 ALLDLKNSN--PEPDPVERVEDRDNA-GALPVRGVWRNGAQKLFS 820
           ALLDLKNSN   +PDPVERVE R+NA G LPVRGVWRNGAQ+LFS
Sbjct: 781 ALLDLKNSNLTSDPDPVERVEGRNNATGVLPVRGVWRNGAQRLFS 823

BLAST of Lag0002699 vs. NCBI nr
Match: XP_004135963.1 (E3 ubiquitin-protein ligase ZNF598 [Cucumis sativus] >KGN45064.1 hypothetical protein Csa_015726 [Cucumis sativus])

HSP 1 Score: 1442.9 bits (3734), Expect = 0.0e+00
Identity = 744/826 (90.07%), Postives = 773/826 (93.58%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEP+EGR GSYWYHEDTQ FFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRCGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PND SKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQGHFLCEDEACLAKKF+VFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEACLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNLS 360
           RYRRSN+QDNRRGRRTFRRDSSDDLLSLA+QESFE+ANVDD NHD LP GQVASDQ NLS
Sbjct: 301 RYRRSNDQDNRRGRRTFRRDSSDDLLSLALQESFETANVDDNNHDPLPSGQVASDQENLS 360

Query: 361 NIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKDA 420
           N+DPL+E FE+LATTD ESASRYLQALGHSRNSQL QSSFPPLSTA SSSHPKP QDKD 
Sbjct: 361 NVDPLIESFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPNQDKDI 420

Query: 421 IHNNSMAAHLRRQR-NVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHPASSSSGQT 480
           IHNNSMAAHLRRQR NVTVLNSA GWPKSSRAPV PSNNSSQAWPAIN NH ASSSSGQT
Sbjct: 421 IHNNSMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHAASSSSGQT 480

Query: 481 KSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVNE 540
           K V TINNG SVS Y+N AQ+H K RS+SSSGSGSSSRISHSASAP+LTD+A+TEPSVNE
Sbjct: 481 KGVATINNGPSVSAYANAAQMHPKPRSTSSSGSGSSSRISHSASAPNLTDIAHTEPSVNE 540

Query: 541 FPPVSAAQARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQG 600
           FPPVSAA ARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFD DRY+IFKDISAQYRQG
Sbjct: 541 FPPVSAAHARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDQDRYSIFKDISAQYRQG 600

Query: 601 QIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSIQ 660
           QIDTEMYLDCVQQFGLSHL+LELARLCPD QKQKELVETY AS HKDV   NGRAQDSIQ
Sbjct: 601 QIDTEMYLDCVQQFGLSHLLLELARLCPDPQKQKELVETYNASFHKDVFPVNGRAQDSIQ 660

Query: 661 FKDRSKGKKGKGKSMDVVKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRAA 720
            KD+SKGKKGKGKS++ VKDS+SKDK ADSI+SSVRELQSS RP +E+VEVLSKG+YR +
Sbjct: 661 IKDKSKGKKGKGKSIE-VKDSSSKDKLADSIMSSVRELQSSYRPPDEDVEVLSKGEYRTS 720

Query: 721 KGKSKLSSDDQQGGIGGQNSQPSTGLSNQSTGD---GGGGSKQKKKTSKFHRVRLGDGSV 780
           KGK K+SSDDQQGG G Q SQPSTGLSNQSTGD   GGGGSKQKKKTSKFHRVRLGDGSV
Sbjct: 721 KGKLKISSDDQQGGTGRQKSQPSTGLSNQSTGDGGGGGGGSKQKKKTSKFHRVRLGDGSV 780

Query: 781 AALLDLKNSN--PEPDPVERVEDRDN-AGALPVRGVWRNGAQKLFS 820
           AALLDLKNSN   +PDP ERVEDR+N AGALPVRGVWRNGAQKLFS
Sbjct: 781 AALLDLKNSNLGSDPDPDERVEDRNNGAGALPVRGVWRNGAQKLFS 824

BLAST of Lag0002699 vs. NCBI nr
Match: XP_022148262.1 (E3 ubiquitin-protein ligase HEL2 [Momordica charantia])

HSP 1 Score: 1432.9 bits (3708), Expect = 0.0e+00
Identity = 741/866 (85.57%), Postives = 772/866 (89.15%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEPREGR GSYWYHEDTQ FFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PNDG KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRK+FICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQ HFLCEDEACLAKKFIVFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLP-----GGQVASD 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQ SFE+ N DDTNHD LP      GQVASD
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360

Query: 361 QGNLSNIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPM 420
           QGN+SNIDPL+EPFESLATTD ESASRYLQALGHSRNSQL QSSFPPLSTAPSSSHPKPM
Sbjct: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420

Query: 421 QDKDAIHNNSMAAHLRRQRNVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHPASSS 480
            DKDA  NNSMAAHLRRQR VTVLNSAQGWPKS RAPV PSNNSSQAWPAINVNH ASSS
Sbjct: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480

Query: 481 SGQTKSVVTINNGSSVSGYSNVAQVHAKA------------------------------- 540
           SGQ K VVTI+NG SVSGYSN AQ+H KA                               
Sbjct: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540

Query: 541 --------RSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVNEFPPVSAAQARKVPSSSQ 600
                   RSSSSSG GSSSRISHSASAPSLTD+AY EPSVN+FPPVSAAQARK PSSSQ
Sbjct: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600

Query: 601 SSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQGQIDTEMYLDCVQQFGLS 660
           SS+N+EDVQTANKSLVEKIRAALDFD DRYA+FKDISAQYRQG I+T+MYLDCVQQFGLS
Sbjct: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660

Query: 661 HLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSIQFKDRSKGKKGKGKSMDV 720
           HLVLELARLCPD QKQKELVETY ASL KDV+QENGRAQD IQ KD+SKGKKGKGK +D 
Sbjct: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYID- 720

Query: 721 VKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRAAKGKSKLSSDDQQGGIGG 780
            KD++SKDK ADSILSSVRELQS+  PSEE VEVLSKG+YRAAKGKSKLSSD+QQGG GG
Sbjct: 721 AKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGG 780

Query: 781 QNSQPSTGLSNQ-STGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNP--EPDPVE 820
           QNSQPSTGLSNQ +TGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKN NP  +PDPVE
Sbjct: 781 QNSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPVE 840

BLAST of Lag0002699 vs. NCBI nr
Match: TYK10361.1 (E3 ubiquitin-protein ligase HEL2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1431.8 bits (3705), Expect = 0.0e+00
Identity = 740/825 (89.70%), Postives = 770/825 (93.33%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFIC DRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICSDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEP+EGR+GSYWYHEDTQ FFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRSGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PND SKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQGHFLCEDE CLAKKF+VFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEGCLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNLS 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFE+ANVDD NHD LP GQVASDQ N+S
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFETANVDDNNHDPLPSGQVASDQENVS 360

Query: 361 NIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKDA 420
           N+DPL+EPFE+LATTD ESASRYLQALGHSRNSQL QSSFPPLSTA SSSHPKP QDKD 
Sbjct: 361 NVDPLIEPFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPNQDKD- 420

Query: 421 IHNNSMAAHLRRQR-NVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHPASSSSGQT 480
               SMAAHLRRQR NVTVLNSA GWPKSSRAPV PSNNSSQAWPAIN NH ASSSSGQT
Sbjct: 421 ----SMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHSASSSSGQT 480

Query: 481 KSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVNE 540
           K V TINNG SVS Y+N AQ+H K R SSSSGSGSSSRISHSASAP+LTD+A+TEPS+NE
Sbjct: 481 KGVATINNGPSVSAYANAAQMHPKPRCSSSSGSGSSSRISHSASAPNLTDIAHTEPSLNE 540

Query: 541 FPPVSAAQARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQG 600
           FPPVSAAQARKVPSSSQSSMN+EDVQTANKSLVEKIRAALDFD DRY+IFKDISAQYRQG
Sbjct: 541 FPPVSAAQARKVPSSSQSSMNVEDVQTANKSLVEKIRAALDFDQDRYSIFKDISAQYRQG 600

Query: 601 QIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSIQ 660
           QIDTEMYLDCVQQFGLSHL+LELARLCPD QKQKELVETY AS HKDV+  NGRAQDSIQ
Sbjct: 601 QIDTEMYLDCVQQFGLSHLLLELARLCPDPQKQKELVETYNASFHKDVIPVNGRAQDSIQ 660

Query: 661 FKDRSKGKKGKGKSMDVVKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRAA 720
            KD+SKGKKGKGKS++ VKDS+SKDK ADSILSSVRELQSS RPSEE+VEVLSKG+YR +
Sbjct: 661 IKDKSKGKKGKGKSIE-VKDSSSKDKLADSILSSVRELQSSYRPSEEDVEVLSKGEYRTS 720

Query: 721 KGKSKLSSDDQQGGIGGQNSQPSTGLSNQSTGD--GGGGSKQKKKTSKFHRVRLGDGSVA 780
           KGK K+SSDDQQGG GGQ SQPSTGLSNQSTGD  GGGGSKQKKKTSKFHRVRLGDGSVA
Sbjct: 721 KGKLKISSDDQQGGTGGQKSQPSTGLSNQSTGDGGGGGGSKQKKKTSKFHRVRLGDGSVA 780

Query: 781 ALLDLKNSN--PEPDPVERVEDRDN-AGALPVRGVWRNGAQKLFS 820
           ALLDLKNSN   +PDP E VEDR+N AGALPVRGVWRNGA +LFS
Sbjct: 781 ALLDLKNSNLTSDPDPDESVEDRNNAAGALPVRGVWRNGAHRLFS 818

BLAST of Lag0002699 vs. NCBI nr
Match: XP_008461386.1 (PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HEL2-like [Cucumis melo])

HSP 1 Score: 1427.9 bits (3695), Expect = 0.0e+00
Identity = 738/825 (89.45%), Postives = 769/825 (93.21%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFIC DRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICSDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEP+EGR+GSYWYHEDTQ FFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRSGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PND SKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQGHFLCEDE CLAKKF+VFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEGCLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNLS 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFE+ANVDD NHD LP GQVASDQ N+S
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFETANVDDNNHDPLPSGQVASDQENVS 360

Query: 361 NIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKDA 420
           N+DPL+EPFE+LATTD ESASRYLQALGHSRNSQL QSSFPPLSTA SSSHPKP QDKD 
Sbjct: 361 NVDPLIEPFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPNQDKD- 420

Query: 421 IHNNSMAAHLRRQR-NVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHPASSSSGQT 480
               SMAAHLRRQR NVTVLNSA GWPKSSRAPV PSNNSSQAWPAIN NH ASSSSGQT
Sbjct: 421 ----SMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHSASSSSGQT 480

Query: 481 KSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVNE 540
           K V TINNG SVS Y+N AQ+H K RSSSSSGSGSSSRISHSASAP+LTD+A+TEPS+NE
Sbjct: 481 KGVATINNGPSVSAYANAAQMHPKPRSSSSSGSGSSSRISHSASAPNLTDIAHTEPSLNE 540

Query: 541 FPPVSAAQARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQG 600
           FPPVSAAQARKVPSSSQSSMN+EDVQTANKSLVEKIRAALDFD DRY+IFKDISAQYRQG
Sbjct: 541 FPPVSAAQARKVPSSSQSSMNVEDVQTANKSLVEKIRAALDFDQDRYSIFKDISAQYRQG 600

Query: 601 QIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSIQ 660
           QIDTEMYLDCVQQFGLSHL+LELARLCPD QKQKELVETY AS HKDV+  NGRAQDSIQ
Sbjct: 601 QIDTEMYLDCVQQFGLSHLLLELARLCPDPQKQKELVETYNASFHKDVIPVNGRAQDSIQ 660

Query: 661 FKDRSKGKKGKGKSMDVVKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRAA 720
            KD+SKGKK KGKS++ VKDS+SKDK ADSILSSVRELQSS RPSEE+VEVLSKG+YR +
Sbjct: 661 IKDKSKGKKXKGKSIE-VKDSSSKDKLADSILSSVRELQSSYRPSEEDVEVLSKGEYRTS 720

Query: 721 KGKSKLSSDDQQGGIGGQNSQPSTGLSNQSTGD--GGGGSKQKKKTSKFHRVRLGDGSVA 780
           KGK K+SSDDQQGG GGQ SQPSTGLSNQSTGD  GGGG + KKKTSKFHRVRLGDGSVA
Sbjct: 721 KGKLKISSDDQQGGTGGQKSQPSTGLSNQSTGDGGGGGGEQTKKKTSKFHRVRLGDGSVA 780

Query: 781 ALLDLKNSN--PEPDPVERVEDRDN-AGALPVRGVWRNGAQKLFS 820
           ALLDLKNSN   +PDP ERVEDR+N AGALPVRGVWRNGA +LFS
Sbjct: 781 ALLDLKNSNLTSDPDPDERVEDRNNAAGALPVRGVWRNGAHRLFS 818

BLAST of Lag0002699 vs. ExPASy Swiss-Prot
Match: Q76PD2 (E3 ubiquitin-protein ligase hel2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC1223.01 PE=1 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 2.6e-38
Identity = 97/313 (30.99%), Postives = 151/313 (48.24%), Query Frame = 0

Query: 5   CAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGDYTRT 64
           C +CA+ + +     C HR +C  C  RLR +   + C  CK+E   V +TK   D+   
Sbjct: 81  CFICAEGITYSCVLPCNHR-MCHVCALRLRALYKTKECTFCKTEWDTVLITK---DHEID 140

Query: 65  INDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQPNDG 124
           I+D  +     ++ + G  +  E  Q   + +  +   +  C ++C              
Sbjct: 141 IHDVDLAKLPFQDEKLGIVYSDEHAQEESNLLLQFNCPEDACDITC-------------- 200

Query: 125 SKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGDSEVD 184
              +G F     LK H   +H  F C LC++ +KVF  E  L+++  L +H   GD    
Sbjct: 201 ---KGWF----DLKLHAKVKHHKFFCDLCVKNKKVFTHEHTLFSKKGLTKHNEVGD---Q 260

Query: 185 GSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRL-HPGQYEYYKNYDDL 244
           GS+ E  GF GHP CEFC T FY D+EL+ H   +H  C+IC ++     ++Y+KNYD L
Sbjct: 261 GSDLEITGFKGHPKCEFCNTHFYDDDELFKHCREKHERCYICDQVAGRPTHQYFKNYDSL 320

Query: 245 EIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSFRYR 304
           E HF + H++C +  CL +KF+VF +E ++K H   EH    ++ +   A +I   F Y 
Sbjct: 321 ERHFEKDHYICRERECLERKFVVFGTEIDLKAHQLDEHPHNFTQRELREARRIIPQFSYD 365

Query: 305 RSNEQDNRRGRRT 317
                   R  RT
Sbjct: 381 PPGASGRNRRERT 365

BLAST of Lag0002699 vs. ExPASy Swiss-Prot
Match: Q86UK7 (E3 ubiquitin-protein ligase ZNF598 OS=Homo sapiens OX=9606 GN=ZNF598 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 7.2e-33
Identity = 102/386 (26.42%), Postives = 169/386 (43.78%), Query Frame = 0

Query: 4   SCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGDYTR 63
           SC +C   LE  A G C H  VC  C  ++R +C  R C +C+ E   V   K L     
Sbjct: 28  SCVLCCGDLEATALGRCDH-PVCYRCSTKMRVLCEQRYCAVCREELRQVVFGKKL----- 87

Query: 64  TINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQPND 123
               FA  P    +       + +    +F D   Y + + + +  C  C ++       
Sbjct: 88  --PAFATIPIHQLQ-------HEKKYDIYFADGKVYALYRQLLQHECPRCPELPP----- 147

Query: 124 GSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGDSEV 183
                  F     L+ H+  +H+LF C LCL+  ++F  E+K Y+R  L +H   GD + 
Sbjct: 148 -------FSLFGDLEQHMRRQHELFCCRLCLQHLQIFTYERKWYSRKDLARHRMQGDPD- 207

Query: 184 DGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNYDDL 243
               S R    GHP+C+FC   +  ++EL  H+  +HY CH C     G  +YY +Y  L
Sbjct: 208 --DTSHR----GHPLCKFCDERYLDNDELLKHLRRDHYFCHFCD--SDGAQDYYSDYAYL 267

Query: 244 EIHFRQGHFLCEDEACLAKKFI-VFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF-- 303
             HFR+ HFLCE+  C  ++F   F++E ++K H T  H    + +++N  + +  S+  
Sbjct: 268 REHFREKHFLCEEGRCSTEQFTHAFRTEIDLKAHRTACHSRSRAEARQNRHIDLQFSYAP 327

Query: 304 RYRRSNE----------------------------QDNRRGRRTFRRDSSDDLLSLAIQE 357
           R+ R NE                            Q +RRG   ++R+  D  ++ A++ 
Sbjct: 328 RHSRRNEGVVGGEDYEEVDRYSRQGRVARAGTRGAQQSRRGSWRYKREEEDREVAAAVRA 377

BLAST of Lag0002699 vs. ExPASy Swiss-Prot
Match: Q05580 (E3 ubiquitin-protein ligase HEL2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HEL2 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 3.1e-28
Identity = 145/563 (25.75%), Postives = 241/563 (42.81%), Query Frame = 0

Query: 2   DDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSES-AVVFVTKALGD 61
           ++ C +CA  L +V+   C H+  C  C  R R +   + C IC++E+  V+F  +  GD
Sbjct: 61  NELCVICARKLTYVSLTPCHHK-TCHICGFRQRALYNKKSCLICRTENEEVMFTDRIDGD 120

Query: 62  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSC--SVCDKIGE 121
            +   N F       +  + G  +  E+  T     +   ++K  C LS    VCD    
Sbjct: 121 ISDKYN-FC-----EKNEKYGINFTSEEVAT-----ETLNLLKFFCPLSKDEQVCD---- 180

Query: 122 DQPNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHT 181
                       F + ++   HL   H   +C +C   +  F CE +++ + QL  H   
Sbjct: 181 ------------FGSFKKYNEHLKSEHNRMICLICATHKHAFPCELEIFTQNQLRNHQTK 240

Query: 182 GDSEVDGSESERGGFTGHPMCEFCR-TPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYY 241
           G+SE         GF GHPMC FC    FY D+ELY HM  +H  CHIC +++P   +Y+
Sbjct: 241 GNSE---------GFKGHPMCAFCSGKRFYSDDELYIHMRNQHEKCHICDKMNPASPQYF 300

Query: 242 KNYDDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGG------KLSRSKRN 301
           K+Y+ L  HF+  H++C  + CL  KF+VF+ E E++ H   EHG       K  +S+ +
Sbjct: 301 KDYNQLFDHFKHSHYVCTVQTCLDNKFVVFKDELELQAHILQEHGNILKGKPKFFQSELS 360

Query: 302 AALQIPTSFRYRR--------SNEQDNRRGRRTFRRDSS---DDLLSLA------IQESF 361
             +  P+     R        S+   +  G RT  R +S   +  L LA      ++ S 
Sbjct: 361 TFISAPSRVIRERDDYDLPSISSLPGSSSGSRTDVRSASSPEESRLRLAERAKYYLENSK 420

Query: 362 ESANVDDTNHDSLPGGQVASDQGNLSNIDPLVEP-------FESLATTDAESASRY--LQ 421
           E  N   + ++    G++++++   S      +P         +LA T  +++S+Y  L 
Sbjct: 421 EDFNKFSSYNEDYSKGRLSAEKLLESYKLLFTKPNADVYLLIHNLAETFPKNSSKYNNLN 480

Query: 422 ALGHSRNSQLG-QSSFPPLSTAPSSSHPKPMQDKDAIHNNSMAAHLRRQRNVTVLNSAQG 481
           A+   R   L  Q+S P LS+  S S           ++   A      RN+  L + + 
Sbjct: 481 AIYEQREQTLARQTSLPSLSSDSSLSMSIGRGHWGGTNDGGSAGAALGVRNIKNLPTLKS 540

Query: 482 WPKSSRAPVPPSNNSSQAWPAINVNHPASSSSGQTKSVVTINNGSSVSGYSNVAQVHAKA 528
            P +S  P   +   +   P  N+      S     S  T+          +   + +K 
Sbjct: 541 -PSASYDPFATTVKKNTLRPVQNIKRTTPQSVSYRTSTNTV--------AFSPTYLESKK 576

BLAST of Lag0002699 vs. ExPASy Swiss-Prot
Match: Q80YR4 (E3 ubiquitin-protein ligase ZNF598 OS=Mus musculus OX=10090 GN=Znf598 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 6.6e-26
Identity = 147/603 (24.38%), Postives = 233/603 (38.64%), Query Frame = 0

Query: 4   SCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGDYTR 63
           SC +C   LE  A G C H  VC  C  ++R +C  R C +C+ E   V   K L     
Sbjct: 26  SCVLCCGDLEATALGRCDH-PVCYRCSTKMRVLCEQRYCAVCREELRQVVFGKKL----- 85

Query: 64  TINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQPND 123
               FA+ P    +       + +    +F D   + + + + +  C  C  +       
Sbjct: 86  --PAFALIPIHQLQ-------HEKKYDIYFADGKVFALYRQLLQHECPRCPHLPP----- 145

Query: 124 GSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGDSEV 183
                  F     L+ H+  +H+LF C LCL+  K+F  E+K Y+R  L +H   GD + 
Sbjct: 146 -------FSLFGDLEQHMRKQHELFCCKLCLKHLKIFTYERKWYSRKDLARHRMQGDPD- 205

Query: 184 DGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNYDDL 243
               S R    GHP+C+FC   +  ++EL  H+  +HY CH C     G  +YY +Y  L
Sbjct: 206 --DTSHR----GHPLCKFCDERYLDNDELLKHLRRDHYFCHFCD--SDGAQDYYSDYAYL 265

Query: 244 EIHFRQGHFLCEDEACLAKKFI-VFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF-- 303
             HFR+ HFLCE+  C  ++F   F++E ++K H T  H    + +++N  + +  SF  
Sbjct: 266 REHFREKHFLCEEGRCSTEQFTHAFRTEIDLKAHKTACHSRSRAEARQNRQIDLQFSFAP 325

Query: 304 -------------------RYRRSNE---------QDNRRGRRTFRRDSSDDLL------ 363
                              RY R            Q NRRG   ++R+  D  +      
Sbjct: 326 RHSRRSEGVVSGEDYEEVDRYNRQGRAGRASGRGAQQNRRGSWRYKREEEDREVAAAIRA 385

Query: 364 SLAIQESFESANVDDTNHDSLPGGQVASDQGNLSNIDPLVEPFESLATTDAESASRYLQA 423
           S+A Q+  E+  V+D    S P  + A+     + +         L     E +     +
Sbjct: 386 SVAAQQQEETQRVEDREEGSRPKKEEAA-----ARVPEEPRGHRRLPRAQGEGSG----S 445

Query: 424 LGHSRNSQLGQSSFPPLSTAP----SSSHPKPMQDKDAIHNNSMAAHLRRQRNVTVLNSA 483
              S N  + Q +FP     P    S++ P P  +       S+ A            S 
Sbjct: 446 KEASANGPVSQEAFPATGPGPVVALSNTLPPPSPELKEEDFPSLCASTSSCCTAVTPGSV 505

Query: 484 QGWPKSSRAPVPPSNNSS---QAWPAINVNHPASSSSGQTKSVVTINNGSSVSGYSNVAQ 543
                +   P PP   ++   + +PA+ V+     SS  +  +   N+G S  G      
Sbjct: 506 ---GLALAYPGPPRGKNTFQEEDFPAL-VSSAPKPSSAPSSLISAWNSGCSKKGNLPTPG 565

Query: 544 VHAKARSSS---SSGSGS-SSRISHSASAPSLTDMAYTEPSVNEFPPVSAAQARKVPSSS 559
             A    S     +G GS   R    A          T   +   P   A  +   P+++
Sbjct: 566 SQAVVGGSQPPRKAGKGSRGGRKGGPAPVDEEDSGGLTVQGLRSVPTTVAVSSLLAPATN 579

BLAST of Lag0002699 vs. ExPASy Swiss-Prot
Match: Q6PFK1 (E3 ubiquitin-protein ligase ZNF598 OS=Danio rerio OX=7955 GN=znf598 PE=1 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 6.1e-24
Identity = 141/605 (23.31%), Postives = 242/605 (40.00%), Query Frame = 0

Query: 2   DDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSE-SAVVFVTKALGD 61
           + +C +C   ++  A G C H  VC  C  ++R +C  + C +C+ +   VVF+ K    
Sbjct: 54  ESTCVLCCQDIDLFAVGKCDH-PVCYRCSTKMRVLCEQKYCAVCREQLDKVVFLRKPEAF 113

Query: 62  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 121
            T  I+                Y   +    +F D   +   + +    C  C      +
Sbjct: 114 ATLNIH---------------HYQCEKKYDIYFGDGKVHAQFRKILLNECPHC-----PE 173

Query: 122 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 181
           P   SK        E+L+ H+  +H+LF C LCL+  K+F  E+K Y+R  L +H   GD
Sbjct: 174 PKVFSK-------FEELEQHMRKQHELFCCKLCLKHLKIFSYERKWYSRKDLARHRMQGD 233

Query: 182 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 241
            +     S R    GHP+C+FC   +  ++EL  H+  +HY CH C     G  EYY +Y
Sbjct: 234 PD---DTSHR----GHPLCKFCDDRYLDNDELLKHLRRDHYFCHFCDA--DGAQEYYSDY 293

Query: 242 DDLEIHFRQGHFLCEDEACLAKKFI-VFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTS 301
             L  HFR+ H+LCE+  C  ++F   F++E + K H    H    + +++N  + I  +
Sbjct: 294 QYLSEHFRESHYLCEEGRCSTEQFTHAFRTEIDYKAHKAAAHSKNRAEARQNRQIDIQFN 353

Query: 302 FRYRRSNEQD------------NRRGR-------------RTFR--RDSSDDLLSLAIQE 361
           +  R+    D            NR+GR             R++R  R+  D  ++ A++ 
Sbjct: 354 YAPRQQRRNDVGGDDYEEVDRFNRQGRPGRGRGPGGQQNLRSWRYNREEEDREMAAAMRA 413

Query: 362 SFESANVDDTNHDSLPGGQVASDQGNLS-NIDPLVEPFESLATTDAESASRYLQALGHSR 421
           S  S + ++ +H        A ++  L    +  +EP E+         +   QA     
Sbjct: 414 SMAS-HQEERSH--------AQERSMLKPRREEKLEPDETRNNRSTARPTNDTQARSMKS 473

Query: 422 NSQLGQSSFPPLSTAPSSSHPKPMQDKDAIHNNSMAAHLRRQRNVTVLNSAQGWPKSSRA 481
           N  L    FP L    + + P P+Q      + S+        + +V++S    P  +  
Sbjct: 474 NGSLAGQDFPVLG---AGAPPAPVQSMIQKPSVSLKEDDFPSLSGSVVSSPM-TPAYTNQ 533

Query: 482 PVPPSNNSSQAWPAINVNHPASSSSGQTKSVVTINNGSSVSGYSNVAQVHAKARSSSSSG 541
           P   S+   + +PA+              S  +      V   +    +  K    SSS 
Sbjct: 534 PRKHSSFQEEDFPALVSKIKPLKPQSSAASAWSQAGSKPVVAPNKPVVLPTKMTPMSSSS 593

Query: 542 SGSSSRISHSASAPSLTDMAYTEPSVNEFPPVSAAQARKVPSSSQSSMNMEDVQTANKSL 577
             SS+    SAS P       T  S      ++  ++ K P   +   + +D    +   
Sbjct: 594 ILSSTDPLPSASVP----QPLTASSSRRKKMLTLTESHKDPPKIRCPSSSDDEDPHSGKT 604

BLAST of Lag0002699 vs. ExPASy TrEMBL
Match: A0A0A0K864 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G419600 PE=4 SV=1)

HSP 1 Score: 1442.9 bits (3734), Expect = 0.0e+00
Identity = 744/826 (90.07%), Postives = 773/826 (93.58%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEP+EGR GSYWYHEDTQ FFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRCGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PND SKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQGHFLCEDEACLAKKF+VFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEACLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNLS 360
           RYRRSN+QDNRRGRRTFRRDSSDDLLSLA+QESFE+ANVDD NHD LP GQVASDQ NLS
Sbjct: 301 RYRRSNDQDNRRGRRTFRRDSSDDLLSLALQESFETANVDDNNHDPLPSGQVASDQENLS 360

Query: 361 NIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKDA 420
           N+DPL+E FE+LATTD ESASRYLQALGHSRNSQL QSSFPPLSTA SSSHPKP QDKD 
Sbjct: 361 NVDPLIESFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPNQDKDI 420

Query: 421 IHNNSMAAHLRRQR-NVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHPASSSSGQT 480
           IHNNSMAAHLRRQR NVTVLNSA GWPKSSRAPV PSNNSSQAWPAIN NH ASSSSGQT
Sbjct: 421 IHNNSMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHAASSSSGQT 480

Query: 481 KSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVNE 540
           K V TINNG SVS Y+N AQ+H K RS+SSSGSGSSSRISHSASAP+LTD+A+TEPSVNE
Sbjct: 481 KGVATINNGPSVSAYANAAQMHPKPRSTSSSGSGSSSRISHSASAPNLTDIAHTEPSVNE 540

Query: 541 FPPVSAAQARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQG 600
           FPPVSAA ARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFD DRY+IFKDISAQYRQG
Sbjct: 541 FPPVSAAHARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDQDRYSIFKDISAQYRQG 600

Query: 601 QIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSIQ 660
           QIDTEMYLDCVQQFGLSHL+LELARLCPD QKQKELVETY AS HKDV   NGRAQDSIQ
Sbjct: 601 QIDTEMYLDCVQQFGLSHLLLELARLCPDPQKQKELVETYNASFHKDVFPVNGRAQDSIQ 660

Query: 661 FKDRSKGKKGKGKSMDVVKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRAA 720
            KD+SKGKKGKGKS++ VKDS+SKDK ADSI+SSVRELQSS RP +E+VEVLSKG+YR +
Sbjct: 661 IKDKSKGKKGKGKSIE-VKDSSSKDKLADSIMSSVRELQSSYRPPDEDVEVLSKGEYRTS 720

Query: 721 KGKSKLSSDDQQGGIGGQNSQPSTGLSNQSTGD---GGGGSKQKKKTSKFHRVRLGDGSV 780
           KGK K+SSDDQQGG G Q SQPSTGLSNQSTGD   GGGGSKQKKKTSKFHRVRLGDGSV
Sbjct: 721 KGKLKISSDDQQGGTGRQKSQPSTGLSNQSTGDGGGGGGGSKQKKKTSKFHRVRLGDGSV 780

Query: 781 AALLDLKNSN--PEPDPVERVEDRDN-AGALPVRGVWRNGAQKLFS 820
           AALLDLKNSN   +PDP ERVEDR+N AGALPVRGVWRNGAQKLFS
Sbjct: 781 AALLDLKNSNLGSDPDPDERVEDRNNGAGALPVRGVWRNGAQKLFS 824

BLAST of Lag0002699 vs. ExPASy TrEMBL
Match: A0A6J1D4L3 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016965 PE=4 SV=1)

HSP 1 Score: 1432.9 bits (3708), Expect = 0.0e+00
Identity = 741/866 (85.57%), Postives = 772/866 (89.15%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEPREGR GSYWYHEDTQ FFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PNDG KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRK+FICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQ HFLCEDEACLAKKFIVFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLP-----GGQVASD 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQ SFE+ N DDTNHD LP      GQVASD
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360

Query: 361 QGNLSNIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPM 420
           QGN+SNIDPL+EPFESLATTD ESASRYLQALGHSRNSQL QSSFPPLSTAPSSSHPKPM
Sbjct: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420

Query: 421 QDKDAIHNNSMAAHLRRQRNVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHPASSS 480
            DKDA  NNSMAAHLRRQR VTVLNSAQGWPKS RAPV PSNNSSQAWPAINVNH ASSS
Sbjct: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480

Query: 481 SGQTKSVVTINNGSSVSGYSNVAQVHAKA------------------------------- 540
           SGQ K VVTI+NG SVSGYSN AQ+H KA                               
Sbjct: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540

Query: 541 --------RSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVNEFPPVSAAQARKVPSSSQ 600
                   RSSSSSG GSSSRISHSASAPSLTD+AY EPSVN+FPPVSAAQARK PSSSQ
Sbjct: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600

Query: 601 SSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQGQIDTEMYLDCVQQFGLS 660
           SS+N+EDVQTANKSLVEKIRAALDFD DRYA+FKDISAQYRQG I+T+MYLDCVQQFGLS
Sbjct: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660

Query: 661 HLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSIQFKDRSKGKKGKGKSMDV 720
           HLVLELARLCPD QKQKELVETY ASL KDV+QENGRAQD IQ KD+SKGKKGKGK +D 
Sbjct: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYID- 720

Query: 721 VKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRAAKGKSKLSSDDQQGGIGG 780
            KD++SKDK ADSILSSVRELQS+  PSEE VEVLSKG+YRAAKGKSKLSSD+QQGG GG
Sbjct: 721 AKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGG 780

Query: 781 QNSQPSTGLSNQ-STGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNP--EPDPVE 820
           QNSQPSTGLSNQ +TGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKN NP  +PDPVE
Sbjct: 781 QNSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPVE 840

BLAST of Lag0002699 vs. ExPASy TrEMBL
Match: A0A5D3CF15 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00030 PE=4 SV=1)

HSP 1 Score: 1431.8 bits (3705), Expect = 0.0e+00
Identity = 740/825 (89.70%), Postives = 770/825 (93.33%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFIC DRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICSDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEP+EGR+GSYWYHEDTQ FFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRSGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PND SKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQGHFLCEDE CLAKKF+VFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEGCLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNLS 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFE+ANVDD NHD LP GQVASDQ N+S
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFETANVDDNNHDPLPSGQVASDQENVS 360

Query: 361 NIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKDA 420
           N+DPL+EPFE+LATTD ESASRYLQALGHSRNSQL QSSFPPLSTA SSSHPKP QDKD 
Sbjct: 361 NVDPLIEPFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPNQDKD- 420

Query: 421 IHNNSMAAHLRRQR-NVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHPASSSSGQT 480
               SMAAHLRRQR NVTVLNSA GWPKSSRAPV PSNNSSQAWPAIN NH ASSSSGQT
Sbjct: 421 ----SMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHSASSSSGQT 480

Query: 481 KSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVNE 540
           K V TINNG SVS Y+N AQ+H K R SSSSGSGSSSRISHSASAP+LTD+A+TEPS+NE
Sbjct: 481 KGVATINNGPSVSAYANAAQMHPKPRCSSSSGSGSSSRISHSASAPNLTDIAHTEPSLNE 540

Query: 541 FPPVSAAQARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQG 600
           FPPVSAAQARKVPSSSQSSMN+EDVQTANKSLVEKIRAALDFD DRY+IFKDISAQYRQG
Sbjct: 541 FPPVSAAQARKVPSSSQSSMNVEDVQTANKSLVEKIRAALDFDQDRYSIFKDISAQYRQG 600

Query: 601 QIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSIQ 660
           QIDTEMYLDCVQQFGLSHL+LELARLCPD QKQKELVETY AS HKDV+  NGRAQDSIQ
Sbjct: 601 QIDTEMYLDCVQQFGLSHLLLELARLCPDPQKQKELVETYNASFHKDVIPVNGRAQDSIQ 660

Query: 661 FKDRSKGKKGKGKSMDVVKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRAA 720
            KD+SKGKKGKGKS++ VKDS+SKDK ADSILSSVRELQSS RPSEE+VEVLSKG+YR +
Sbjct: 661 IKDKSKGKKGKGKSIE-VKDSSSKDKLADSILSSVRELQSSYRPSEEDVEVLSKGEYRTS 720

Query: 721 KGKSKLSSDDQQGGIGGQNSQPSTGLSNQSTGD--GGGGSKQKKKTSKFHRVRLGDGSVA 780
           KGK K+SSDDQQGG GGQ SQPSTGLSNQSTGD  GGGGSKQKKKTSKFHRVRLGDGSVA
Sbjct: 721 KGKLKISSDDQQGGTGGQKSQPSTGLSNQSTGDGGGGGGSKQKKKTSKFHRVRLGDGSVA 780

Query: 781 ALLDLKNSN--PEPDPVERVEDRDN-AGALPVRGVWRNGAQKLFS 820
           ALLDLKNSN   +PDP E VEDR+N AGALPVRGVWRNGA +LFS
Sbjct: 781 ALLDLKNSNLTSDPDPDESVEDRNNAAGALPVRGVWRNGAHRLFS 818

BLAST of Lag0002699 vs. ExPASy TrEMBL
Match: A0A1S3CEL8 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499981 PE=4 SV=1)

HSP 1 Score: 1427.9 bits (3695), Expect = 0.0e+00
Identity = 738/825 (89.45%), Postives = 769/825 (93.21%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFIC DRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICSDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEP+EGR+GSYWYHEDTQ FFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRSGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PND SKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQGHFLCEDE CLAKKF+VFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEGCLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNLS 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFE+ANVDD NHD LP GQVASDQ N+S
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFETANVDDNNHDPLPSGQVASDQENVS 360

Query: 361 NIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKDA 420
           N+DPL+EPFE+LATTD ESASRYLQALGHSRNSQL QSSFPPLSTA SSSHPKP QDKD 
Sbjct: 361 NVDPLIEPFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPNQDKD- 420

Query: 421 IHNNSMAAHLRRQR-NVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHPASSSSGQT 480
               SMAAHLRRQR NVTVLNSA GWPKSSRAPV PSNNSSQAWPAIN NH ASSSSGQT
Sbjct: 421 ----SMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHSASSSSGQT 480

Query: 481 KSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRISHSASAPSLTDMAYTEPSVNE 540
           K V TINNG SVS Y+N AQ+H K RSSSSSGSGSSSRISHSASAP+LTD+A+TEPS+NE
Sbjct: 481 KGVATINNGPSVSAYANAAQMHPKPRSSSSSGSGSSSRISHSASAPNLTDIAHTEPSLNE 540

Query: 541 FPPVSAAQARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKDISAQYRQG 600
           FPPVSAAQARKVPSSSQSSMN+EDVQTANKSLVEKIRAALDFD DRY+IFKDISAQYRQG
Sbjct: 541 FPPVSAAQARKVPSSSQSSMNVEDVQTANKSLVEKIRAALDFDQDRYSIFKDISAQYRQG 600

Query: 601 QIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQKELVETYKASLHKDVVQENGRAQDSIQ 660
           QIDTEMYLDCVQQFGLSHL+LELARLCPD QKQKELVETY AS HKDV+  NGRAQDSIQ
Sbjct: 601 QIDTEMYLDCVQQFGLSHLLLELARLCPDPQKQKELVETYNASFHKDVIPVNGRAQDSIQ 660

Query: 661 FKDRSKGKKGKGKSMDVVKDSTSKDKFADSILSSVRELQSSLRPSEENVEVLSKGDYRAA 720
            KD+SKGKK KGKS++ VKDS+SKDK ADSILSSVRELQSS RPSEE+VEVLSKG+YR +
Sbjct: 661 IKDKSKGKKXKGKSIE-VKDSSSKDKLADSILSSVRELQSSYRPSEEDVEVLSKGEYRTS 720

Query: 721 KGKSKLSSDDQQGGIGGQNSQPSTGLSNQSTGD--GGGGSKQKKKTSKFHRVRLGDGSVA 780
           KGK K+SSDDQQGG GGQ SQPSTGLSNQSTGD  GGGG + KKKTSKFHRVRLGDGSVA
Sbjct: 721 KGKLKISSDDQQGGTGGQKSQPSTGLSNQSTGDGGGGGGEQTKKKTSKFHRVRLGDGSVA 780

Query: 781 ALLDLKNSN--PEPDPVERVEDRDN-AGALPVRGVWRNGAQKLFS 820
           ALLDLKNSN   +PDP ERVEDR+N AGALPVRGVWRNGA +LFS
Sbjct: 781 ALLDLKNSNLTSDPDPDERVEDRNNAAGALPVRGVWRNGAHRLFS 818

BLAST of Lag0002699 vs. ExPASy TrEMBL
Match: A0A5A7URS1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00300 PE=4 SV=1)

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 740/833 (88.84%), Postives = 770/833 (92.44%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFIC DRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICSDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF VFPSEP+EGR+GSYWYHEDTQ FFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRSGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180
           PND SKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLE--------IHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNA 300
           DDLE        IHFRQGHFLCEDE CLAKKF+VFQSEAEMKRHNTIEHGGKLSRSKRNA
Sbjct: 241 DDLEAMSFFWRQIHFRQGHFLCEDEGCLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNA 300

Query: 301 ALQIPTSFRYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQV 360
           ALQIPTSFRYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFE+ANVDD NHD LP GQV
Sbjct: 301 ALQIPTSFRYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFETANVDDNNHDPLPSGQV 360

Query: 361 ASDQGNLSNIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHP 420
           ASDQ N+SN+DPL+EPFE+LATTD ESASRYLQALGHSRNSQL QSSFPPLSTA SSSHP
Sbjct: 361 ASDQENVSNVDPLIEPFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHP 420

Query: 421 KPMQDKDAIHNNSMAAHLRRQR-NVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAINVNHP 480
           KP QDKD     SMAAHLRRQR NVTVLNSA GWPKSSRAPV PSNNSSQAWPAIN NH 
Sbjct: 421 KPNQDKD-----SMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHS 480

Query: 481 ASSSSGQTKSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRISHSASAPSLTDMA 540
           ASSSSGQTK V TINNG SVS Y+N AQ+H K R SSSSGSGSSSRISHSASAP+LTD+A
Sbjct: 481 ASSSSGQTKGVATINNGPSVSAYANAAQMHPKPRCSSSSGSGSSSRISHSASAPNLTDIA 540

Query: 541 YTEPSVNEFPPVSAAQARKVPSSSQSSMNMEDVQTANKSLVEKIRAALDFDPDRYAIFKD 600
           +TEPS+NEFPPVSAAQARKVPSSSQSSMN+EDVQTANKSLVEKIRAALDFD DRY+IFKD
Sbjct: 541 HTEPSLNEFPPVSAAQARKVPSSSQSSMNVEDVQTANKSLVEKIRAALDFDQDRYSIFKD 600

Query: 601 ISAQYRQGQIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQKELVETYKASLHKDVVQEN 660
           ISAQYRQGQIDTEMYLDCVQQFGLSHL+LELARLCPD QKQKELVETY AS HKDV+  N
Sbjct: 601 ISAQYRQGQIDTEMYLDCVQQFGLSHLLLELARLCPDPQKQKELVETYNASFHKDVIPVN 660

Query: 661 GRAQDSIQFKDRSKGKKGKGKSMDVVKDSTSKDKFADSILSSVRELQSSLRPSEENVEVL 720
           GRAQDSIQ KD+SKGKKGKGKS++ VKDS+SKDK ADSILSSVRELQSS RPSEE+VEVL
Sbjct: 661 GRAQDSIQIKDKSKGKKGKGKSIE-VKDSSSKDKLADSILSSVRELQSSYRPSEEDVEVL 720

Query: 721 SKGDYRAAKGKSKLSSDDQQGGIGGQNSQPSTGLSNQSTGD--GGGGSKQKKKTSKFHRV 780
           SKG+YR +KGK K+SSDDQQGG GGQ SQPSTGLSNQSTGD  GGGGSKQKKKTSKFHRV
Sbjct: 721 SKGEYRTSKGKLKISSDDQQGGTGGQKSQPSTGLSNQSTGDGGGGGGSKQKKKTSKFHRV 780

Query: 781 RLGDGSVAALLDLKNSN--PEPDPVERVEDRDN-AGALPVRGVWRNGAQKLFS 820
           RLGDGSVAALLDLKNSN   +PDP E VEDR+N AGALPVRGVWRNGA +LFS
Sbjct: 781 RLGDGSVAALLDLKNSNLTSDPDPDESVEDRNNAAGALPVRGVWRNGAHRLFS 826

BLAST of Lag0002699 vs. TAIR 10
Match: AT3G62240.1 (RING/U-box superfamily protein )

HSP 1 Score: 858.6 bits (2217), Expect = 4.1e-249
Identity = 480/855 (56.14%), Postives = 603/855 (70.53%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCAD LEWVAYGSCGHR+VCSTCV RLRF+  D RCCICK+ES +VFVTKALGD
Sbjct: 1   MDDSCAVCADNLEWVAYGSCGHREVCSTCVVRLRFVLDDPRCCICKTESPIVFVTKALGD 60

Query: 61  YTRTINDFAVFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF+ FPS PREGR G++WYHEDTQ FFDD+D Y+MIKAMCRLSC VCDK  ED+
Sbjct: 61  YTRTINDFSTFPSAPREGRVGAFWYHEDTQAFFDDLDQYRMIKAMCRLSCGVCDKT-EDK 120

Query: 121 PNDGSKR--RGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHT 180
           P +G  R  R R +++EQLKGHL+H+HKL MC LCLEGRK+FICEQKLY RAQLNQHI T
Sbjct: 121 PREGGPRHHRQRIKSVEQLKGHLYHKHKLHMCGLCLEGRKIFICEQKLYTRAQLNQHIQT 180

Query: 181 GDSEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYK 240
           GDSEVDGSESERGGF GHPMCEFCR PFYGDNELYTHMSTEHYTCH+CQR  PGQYEYYK
Sbjct: 181 GDSEVDGSESERGGFAGHPMCEFCRNPFYGDNELYTHMSTEHYTCHLCQRSQPGQYEYYK 240

Query: 241 NYDDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPT 300
           NYDDLEIHFR+ HFLCED++CLAKKF VFQ+E+E+KRHN IEHGGK+SRS+R+AALQIPT
Sbjct: 241 NYDDLEIHFRRDHFLCEDDSCLAKKFTVFQNESELKRHNAIEHGGKMSRSQRSAALQIPT 300

Query: 301 SFRYRRSNEQDNRRGR-RTFRRDSSDDLLSLAIQESFESANVDDTNHD------SLPGGQ 360
           SFRY R N+Q+NRRGR R+FRR+  D+  +LA+  +   +  + +  +      S P G 
Sbjct: 301 SFRYSRGNDQENRRGRPRSFRREPGDEEYNLAVHAALRLSESEYSRQEPAPPPSSAPPGF 360

Query: 361 VASDQGNLSNIDPLVEPFESLATTDAESASRYLQALGH--SRNSQLGQSSFPPLSTAPSS 420
             ++  ++ + DPL++P ESL+TTD E +SRYLQA+G      S+LG+S+FPPLS   SS
Sbjct: 361 SENNNIHVDDTDPLIQPMESLSTTDMEPSSRYLQAVGSFGGGGSRLGESAFPPLSGQQSS 420

Query: 421 SHPKPMQDKDAIHNNSMAAHLRRQRNVT-----VLNSAQGWPKSSRAPVPPS-----NNS 480
                 Q+ +++  N+MAA LRRQ N T     + + +QGWP  +R P   S     N+S
Sbjct: 421 G-----QNVESLPTNTMAARLRRQTNRTSTASAIASPSQGWPVINRGPGQASITSGGNHS 480

Query: 481 SQAWPAI-NVNHPASSSSGQTKSVVTINNGSSVSGYSNVAQVHAKARSSSSSGSGSSSRI 540
           S  WPAI      ASSSS Q++S            ++ V+Q    A S+    + +++RI
Sbjct: 481 SSGWPAIGRTPVQASSSSVQSRS------------HNRVSQPRPLA-SAVPQAARNANRI 540

Query: 541 SHSASAPSLTDMAYTEPSVNEFPPVSAA--QARKVPS-SSQSSMNME---DVQTANKSLV 600
            HS+SAP+L+D    +PS ++FPPVS+A  Q RK  S ++Q S N +   DVQ+ANKSL+
Sbjct: 541 PHSSSAPNLSDTRSLQPSHSDFPPVSSAVVQNRKTSSTTTQGSSNTQPPPDVQSANKSLI 600

Query: 601 EKIRAALDFDPDRYAIFKDISAQYRQGQIDTEMYLDCVQQFGLSHLVLELARLCPDTQKQ 660
           EK+R+AL  D D +  F++IS QYRQG ID + YL+ VQ +GLSHLV++LARLCPD ++Q
Sbjct: 601 EKMRSALGHDEDVFVAFRNISGQYRQGSIDAKTYLEYVQGYGLSHLVIDLARLCPDPKRQ 660

Query: 661 KELVETYKASLHKDVVQENGR--AQDSIQFKDRSKGKKGKGKSMDVVKDSTSKDKFADSI 720
           KEL++T+ ASL ++  ++NGR  AQ S Q K+    KK KGK++ VV     K+  AD+ 
Sbjct: 661 KELIDTHNASLREEDSKDNGRSAAQSSSQPKESQSSKKNKGKAVKVV---DPKETLADNF 720

Query: 721 LSSVRELQSSLRPSEENVEVLS--KGDYRAAKGKSKLSSDDQQGGIGGQNSQPSTGLSNQ 780
           + +VR LQSS  P EE  E +S  K  YR+ KGKS++                  G  + 
Sbjct: 721 MDTVRRLQSSQNPQEEEEEAISKDKNTYRSDKGKSQV-----------------VGTDSS 780

Query: 781 STGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNPEPDPVER----VEDRDNAGAL 820
           ST    G  +Q+KKTSKFHRVRLGDGS+AALLDL NS  E +   +      +++  G L
Sbjct: 781 ST----GSKQQRKKTSKFHRVRLGDGSMAALLDLNNSTRESEQESKDSNSNSNQNQTGGL 812

BLAST of Lag0002699 vs. TAIR 10
Match: AT2G47090.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 661.4 bits (1705), Expect = 9.6e-190
Identity = 394/856 (46.03%), Postives = 526/856 (61.45%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCA+ LEWV YGSCGHR+VCSTCV RLRFI  DRRCCICK+E  VVFVTKALGD
Sbjct: 1   MDDSCAVCAENLEWVGYGSCGHREVCSTCVVRLRFILNDRRCCICKTECPVVFVTKALGD 60

Query: 61  YTRTINDFA-VFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGED 120
           YT+TI+DF+  FPS P+EGR GS+WYHE+T  +FDD++HY  IKAMCRLSC++C+   + 
Sbjct: 61  YTKTISDFSTTFPSVPKEGRVGSFWYHEETNVYFDDLNHYTRIKAMCRLSCNLCNDTNKT 120

Query: 121 QPNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTG 180
           +P        RF+++E LK HL H+HKL MCSLCL GRKVFICEQKL+ + QLNQHI +G
Sbjct: 121 RPKKEPNHCVRFKSVEHLKDHLNHQHKLHMCSLCLVGRKVFICEQKLFTKGQLNQHISSG 180

Query: 181 DSEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKN 240
           DSEVDGSESERGGFTGHPMCEFC+ PFYGDNELYTHMS EHYTCHICQRL PGQYEYY N
Sbjct: 181 DSEVDGSESERGGFTGHPMCEFCKRPFYGDNELYTHMSREHYTCHICQRLKPGQYEYYGN 240

Query: 241 YDDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTS 300
           YDDLE+HFR  HFLCEDE CLAKKFIVFQ EAE+KRHN I+HGG++SRS++NA+LQI  S
Sbjct: 241 YDDLEVHFRSDHFLCEDETCLAKKFIVFQIEAELKRHNAIDHGGRMSRSQQNASLQIQAS 300

Query: 301 FRYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNL 360
           F+Y  S     RRGRR     SS    +L + ES  S   +D N  +LP           
Sbjct: 301 FQYPNS-----RRGRRR----SSLREPNLVLLESQASYAFNDDN--NLP----------- 360

Query: 361 SNIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKD 420
                                    Q +G S NS+LG+SSFPPLS   +    +  Q+ +
Sbjct: 361 -------------------------QHVGRSGNSRLGESSFPPLSVQANQGQSRFGQNSE 420

Query: 421 AIHNNSMAAHLRR----------QRNVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAIN-- 480
           ++ +N+     R           Q + +++++     +  +     ++ SSQAWPA+N  
Sbjct: 421 SLVSNTTTTRQRHRANQGQSRFGQNSESLVSNTTTTRQRHQTNRSATSGSSQAWPALNRG 480

Query: 481 ---------VNHPASSSSGQTKSVVTINN----GSSVSGYSNVAQVHAKARSSSSSGSGS 540
                    V    +S+  Q++    + +     S+V   +        + SS SS + +
Sbjct: 481 PAEISITSRVQSSGASAQSQSRHHDRVESTRTLASAVPQDARTTVGGCSSGSSLSSANAT 540

Query: 541 SSRISHSASAPSLTD-MAYTEPSVNEFPPVSAAQARKVPSSSQSSMNME------DVQTA 600
                HS+S P +++  +  +PS ++ P +SA + R+  S+S ++ N++      DVQ+ 
Sbjct: 541 KRNNHHSSSTPKMSETRSLAQPSHSDSPQISAVKNRRSSSTSANAGNIQVAQGVSDVQSD 600

Query: 601 NKSLVEKIRAALDFDPDRYAIFKDISAQYRQGQIDTEMYLDCVQQFGLSHLVLELARLCP 660
           NKSLVEKI A+L  D + +  FK+ S +YR G ID   YL+ V+ +GLSHLVL++ARLCP
Sbjct: 601 NKSLVEKIHASLGHDEELFMAFKNTSGKYRHGSIDARTYLEYVKGYGLSHLVLDMARLCP 660

Query: 661 DTQKQKELVETYKASLHKDVVQENGRAQDSIQFKDRSKGKKGKGKSMDVVKDSTSKDKFA 720
           D Q+QKEL++T+ A L                      G KGK      VK  +S D   
Sbjct: 661 DPQRQKELIDTHNACL--------------------KGGNKGK-----AVKVESSSDSKG 720

Query: 721 DSILSSVRELQSSLRPSEENVEVLSKGDYRAAKGKSKLSSDDQQGGIGGQNSQPSTGLSN 780
           D  + +VR+LQ S +  +++ +   K  YR+ KGK+K+                +T +++
Sbjct: 721 DRFVDTVRKLQFSDKSQDKDKD---KDAYRSDKGKTKV----------------TTLVNS 765

Query: 781 QSTGDG-GGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNPEPDPVERVED----RDNAG 819
            S G G G   KQ KKTSKF R RLG+ S+AA+LDL+NSNPEP+P  + ++    +++ G
Sbjct: 781 SSAGVGLGDTGKQPKKTSKFLRTRLGEKSMAAVLDLRNSNPEPEPEPKNDNSKRSQNSPG 765

BLAST of Lag0002699 vs. TAIR 10
Match: AT2G47090.2 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 611.3 bits (1575), Expect = 1.1e-174
Identity = 379/856 (44.28%), Postives = 507/856 (59.23%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCA+ LEWV YGSCGHR+VCSTCV RLRFI  DRRCCICK+E  VVFVTKALGD
Sbjct: 1   MDDSCAVCAENLEWVGYGSCGHREVCSTCVVRLRFILNDRRCCICKTECPVVFVTKALGD 60

Query: 61  YTRTINDFA-VFPSEPREGRNGSYWYHEDTQTFFDDVDHYKMIKAMCRLSCSVCDKIGED 120
           YT+TI+DF+  FPS P+EGR GS+W                       LSC++C+   + 
Sbjct: 61  YTKTISDFSTTFPSVPKEGRVGSFW-----------------------LSCNLCNDTNKT 120

Query: 121 QPNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTG 180
           +P        RF+++E LK HL H+HKL MCSLCL GRKVFICEQKL+ + QLNQHI +G
Sbjct: 121 RPKKEPNHCVRFKSVEHLKDHLNHQHKLHMCSLCLVGRKVFICEQKLFTKGQLNQHISSG 180

Query: 181 DSEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKN 240
           DSEVDGSESERGGFTGHPMCEFC+ PFYGDNELYTHMS EHYTCHICQRL PGQYEYY N
Sbjct: 181 DSEVDGSESERGGFTGHPMCEFCKRPFYGDNELYTHMSREHYTCHICQRLKPGQYEYYGN 240

Query: 241 YDDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTS 300
           YDDLE+HFR  HFLCEDE CLAKKFIVFQ EAE+KRHN I+HGG++SRS++NA+LQI  S
Sbjct: 241 YDDLEVHFRSDHFLCEDETCLAKKFIVFQIEAELKRHNAIDHGGRMSRSQQNASLQIQAS 300

Query: 301 FRYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFESANVDDTNHDSLPGGQVASDQGNL 360
           F+Y  S     RRGRR     SS    +L + ES  S   +D N  +LP           
Sbjct: 301 FQYPNS-----RRGRRR----SSLREPNLVLLESQASYAFNDDN--NLP----------- 360

Query: 361 SNIDPLVEPFESLATTDAESASRYLQALGHSRNSQLGQSSFPPLSTAPSSSHPKPMQDKD 420
                                    Q +G S NS+LG+SSFPPLS   +    +  Q+ +
Sbjct: 361 -------------------------QHVGRSGNSRLGESSFPPLSVQANQGQSRFGQNSE 420

Query: 421 AIHNNSMAAHLRR----------QRNVTVLNSAQGWPKSSRAPVPPSNNSSQAWPAIN-- 480
           ++ +N+     R           Q + +++++     +  +     ++ SSQAWPA+N  
Sbjct: 421 SLVSNTTTTRQRHRANQGQSRFGQNSESLVSNTTTTRQRHQTNRSATSGSSQAWPALNRG 480

Query: 481 ---------VNHPASSSSGQTKSVVTINN----GSSVSGYSNVAQVHAKARSSSSSGSGS 540
                    V    +S+  Q++    + +     S+V   +        + SS SS + +
Sbjct: 481 PAEISITSRVQSSGASAQSQSRHHDRVESTRTLASAVPQDARTTVGGCSSGSSLSSANAT 540

Query: 541 SSRISHSASAPSLTD-MAYTEPSVNEFPPVSAAQARKVPSSSQSSMNME------DVQTA 600
                HS+S P +++  +  +PS ++ P +SA + R+  S+S ++ N++      DVQ+ 
Sbjct: 541 KRNNHHSSSTPKMSETRSLAQPSHSDSPQISAVKNRRSSSTSANAGNIQVAQGVSDVQSD 600

Query: 601 NKSLVEKIRAALDFDPDRYAIFKDISAQYRQGQIDTEMYLDCVQQFGLSHLVLELARLCP 660
           NKSLVEKI A+L  D + +  FK+ S +YR G ID   YL+ V+ +GLSHLVL++ARLCP
Sbjct: 601 NKSLVEKIHASLGHDEELFMAFKNTSGKYRHGSIDARTYLEYVKGYGLSHLVLDMARLCP 660

Query: 661 DTQKQKELVETYKASLHKDVVQENGRAQDSIQFKDRSKGKKGKGKSMDVVKDSTSKDKFA 720
           D Q+QKEL++T+ A L                      G KGK      VK  +S D   
Sbjct: 661 DPQRQKELIDTHNACL--------------------KGGNKGK-----AVKVESSSDSKG 720

Query: 721 DSILSSVRELQSSLRPSEENVEVLSKGDYRAAKGKSKLSSDDQQGGIGGQNSQPSTGLSN 780
           D  + +VR+LQ S +  +++ +   K  YR+ KGK+K+                +T +++
Sbjct: 721 DRFVDTVRKLQFSDKSQDKDKD---KDAYRSDKGKTKV----------------TTLVNS 742

Query: 781 QSTGDG-GGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNPEPDPVERVED----RDNAG 819
            S G G G   KQ KKTSKF R RLG+ S+AA+LDL+NSNPEP+P  + ++    +++ G
Sbjct: 781 SSAGVGLGDTGKQPKKTSKFLRTRLGEKSMAAVLDLRNSNPEPEPEPKNDNSKRSQNSPG 742

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897973.10.0e+0091.27E3 ubiquitin-protein ligase HEL2 [Benincasa hispida][more]
XP_004135963.10.0e+0090.07E3 ubiquitin-protein ligase ZNF598 [Cucumis sativus] >KGN45064.1 hypothetical pr... [more]
XP_022148262.10.0e+0085.57E3 ubiquitin-protein ligase HEL2 [Momordica charantia][more]
TYK10361.10.0e+0089.70E3 ubiquitin-protein ligase HEL2-like [Cucumis melo var. makuwa][more]
XP_008461386.10.0e+0089.45PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HEL2-like [Cucumis m... [more]
Match NameE-valueIdentityDescription
Q76PD22.6e-3830.99E3 ubiquitin-protein ligase hel2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q86UK77.2e-3326.42E3 ubiquitin-protein ligase ZNF598 OS=Homo sapiens OX=9606 GN=ZNF598 PE=1 SV=1[more]
Q055803.1e-2825.75E3 ubiquitin-protein ligase HEL2 OS=Saccharomyces cerevisiae (strain ATCC 204508... [more]
Q80YR46.6e-2624.38E3 ubiquitin-protein ligase ZNF598 OS=Mus musculus OX=10090 GN=Znf598 PE=1 SV=1[more]
Q6PFK16.1e-2423.31E3 ubiquitin-protein ligase ZNF598 OS=Danio rerio OX=7955 GN=znf598 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0K8640.0e+0090.07RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G419600 PE... [more]
A0A6J1D4L30.0e+0085.57RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101696... [more]
A0A5D3CF150.0e+0089.70RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CEL80.0e+0089.45RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499981 PE=4 ... [more]
A0A5A7URS10.0e+0088.84RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT3G62240.14.1e-24956.14RING/U-box superfamily protein [more]
AT2G47090.19.6e-19046.03zinc ion binding;nucleic acid binding [more]
AT2G47090.21.1e-17444.28zinc ion binding;nucleic acid binding [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013087Zinc finger C2H2-typeSMARTSM00355c2h2final6coord: 221..251
e-value: 18.0
score: 11.1
coord: 108..145
e-value: 61.0
score: 6.3
coord: 197..220
e-value: 15.0
score: 11.8
coord: 252..281
e-value: 96.0
score: 4.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 502..526
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 337..361
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 391..419
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 444..484
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 725..757
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 787..801
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 337..363
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 654..679
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 718..819
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 391..412
NoneNo IPR availablePANTHERPTHR22938:SF14EBR1coord: 1..817
IPR044288E3 ubiquitin-protein ligase ZNF598/Hel2PANTHERPTHR22938ZINC FINGER PROTEIN 598coord: 1..817
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 5..46
score: 9.214112
IPR041888ZN598/Hel2, RING fingerCDDcd16615RING-HC_ZNF598coord: 5..46
e-value: 7.72808E-16
score: 70.0035

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002699.1Lag0002699.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0072344 rescue of stalled ribosome
molecular_function GO:0046872 metal ion binding
molecular_function GO:0043022 ribosome binding
molecular_function GO:0061630 ubiquitin protein ligase activity