Lag0002671 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002671
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	nifU-like protein 1, chloroplastic
Location	chr4: 44694370 .. 44694717 (+)
RNA-Seq Expression	Lag0002671
Synteny	Lag0002671

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCTTTAACTACCTCCGGCCTGCTCAAAACCCCTCACTTTGCAAATTATCGCCAATACCCACCTTCTGCTTATCGGCAACATCAAGGATCCATTTCCCTGAAAAGGGCTTTTCCCAGAAGAGCCATTAGAGCTTCAGCTTCGAATTCGGGTCCATCCACAAGCTCGTCGCCAGGGTTGTACTCAGCCCAGAAATTTGAACTGACCATTGGAAACGTGGACTTGGTTCTCGAGGATGTGAGGCCATATCTAATCGCCGATGGAGGAAATGTCGATGTTGTATCTGTCGAGGACGGTGTGGTTTCTCTCAAACTCGTTGGTGGGTGGATTTCTCATCTCATTTAA

mRNA sequence

ATGGCGTCTTTAACTACCTCCGGCCTGCTCAAAACCCCTCACTTTGCAAATTATCGCCAATACCCACCTTCTGCTTATCGGCAACATCAAGGATCCATTTCCCTGAAAAGGGCTTTTCCCAGAAGAGCCATTAGAGCTTCAGCTTCGAATTCGGGTCCATCCACAAGCTCGTCGCCAGGGTTGTACTCAGCCCAGAAATTTGAACTGACCATTGGAAACGTGGACTTGGTTCTCGAGGATGTGAGGCCATATCTAATCGCCGATGGAGGAAATGTCGATGTTGTATCTGTCGAGGACGGTGTGGTTTCTCTCAAACTCGTTGGTGGGTGGATTTCTCATCTCATTTAA

Coding sequence (CDS)

ATGGCGTCTTTAACTACCTCCGGCCTGCTCAAAACCCCTCACTTTGCAAATTATCGCCAATACCCACCTTCTGCTTATCGGCAACATCAAGGATCCATTTCCCTGAAAAGGGCTTTTCCCAGAAGAGCCATTAGAGCTTCAGCTTCGAATTCGGGTCCATCCACAAGCTCGTCGCCAGGGTTGTACTCAGCCCAGAAATTTGAACTGACCATTGGAAACGTGGACTTGGTTCTCGAGGATGTGAGGCCATATCTAATCGCCGATGGAGGAAATGTCGATGTTGTATCTGTCGAGGACGGTGTGGTTTCTCTCAAACTCGTTGGTGGGTGGATTTCTCATCTCATTTAA

Protein sequence

MASLTTSGLLKTPHFANYRQYPPSAYRQHQGSISLKRAFPRRAIRASASNSGPSTSSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGGWISHLI

Homology

[Close]

BLAST of Lag0002671 vs. NCBI nr
Match: XP_022144330.1 (nifU-like protein 1, chloroplastic [Momordica charantia])

HSP 1 Score: 183.3 bits (464), Expect = 1.2e-42
Identity = 99/113 (87.61%), Postives = 101/113 (89.38%), Query Frame = 0


Query: 1   MASLTTSGLLKTPHF-----ANYRQYPPSAYRQHQGSISLKRAFPRRAIRASASNSGPST 60
           MASLTTSGLLKTP       ANYRQYPPSA+RQHQG  SLKR  PRR I+ASASNSGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG 109
           SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG 113

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BLAST of Lag0002671 vs. NCBI nr
Match: KAE8646369.1 (hypothetical protein Csa_016477 [Cucumis sativus])

HSP 1 Score: 178.3 bits (451), Expect = 3.7e-41
Identity = 100/119 (84.03%), Postives = 102/119 (85.71%), Query Frame = 0


Query: 1   MASLTTSGLLKTP-----HFANYRQYPPSAYRQHQGSISLKRAFPRRAIRASASNSGPST 60
           MASLTTSGLLKTP       AN  QYPP AY QHQ  +SLKRAFP RAIRAS SNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG-GWISH 114
           +SSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG GW SH
Sbjct: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGHGWGSH 119

[Close]

BLAST of Lag0002671 vs. NCBI nr
Match: XP_008451442.1 (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo])

HSP 1 Score: 176.8 bits (447), Expect = 1.1e-40
Identity = 97/113 (85.84%), Postives = 99/113 (87.61%), Query Frame = 0


Query: 1   MASLTTSGLLKTP-----HFANYRQYPPSAYRQHQGSISLKRAFPRRAIRASASNSGPST 60
           MASLTTSGLLKTP       ANY QYPP AY QHQ  +SLKRAFP RA+RAS SNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG 109
           SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG 113

[Close]

BLAST of Lag0002671 vs. NCBI nr
Match: KAA0046713.1 (nifU-like protein 1 [Cucumis melo var. makuwa] >TYK18249.1 nifU-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 176.8 bits (447), Expect = 1.1e-40
Identity = 97/113 (85.84%), Postives = 99/113 (87.61%), Query Frame = 0


Query: 1   MASLTTSGLLKTP-----HFANYRQYPPSAYRQHQGSISLKRAFPRRAIRASASNSGPST 60
           MASLTTSGLLKTP       ANY QYPP AY QHQ  +SLKRAFP RA+RAS SNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG 109
           SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG 113

[Close]

BLAST of Lag0002671 vs. NCBI nr
Match: XP_022991837.1 (nifU-like protein 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 173.7 bits (439), Expect = 9.1e-40
Identity = 93/113 (82.30%), Postives = 100/113 (88.50%), Query Frame = 0


Query: 1   MASLTTSGLLKTPHF-----ANYRQYPPSAYRQHQGSISLKRAFPRRAIRASASNSGPST 60
           MASLT SGLLKTP +     AN+RQYPPSA+ QHQ  +SL RA PR++IRASASNSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG 109
           SSSPGLYSAQKFELTI NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVG
Sbjct: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVG 113

[Close]

BLAST of Lag0002671 vs. ExPASy Swiss-Prot
Match: Q93W77 (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 7.3e-16
Identity = 52/83 (62.65%), Postives = 58/83 (69.88%), Query Frame = 0


Query: 33  ISLKRAFPRRAIRASASN---SGPSTSSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADG 92
           IS    F R AI  S+     S  ++  S GLYSAQ F+LT  NVDLVLEDVRP+LI+DG
Sbjct: 46  ISRTAIFHRSAISGSSQGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLEDVRPFLISDG 105

Query: 93  GNVDVVSVEDGVVSLKLVGGWIS 113
           GNVDVVSVEDGVVSLKL G   S
Sbjct: 106 GNVDVVSVEDGVVSLKLQGACTS 128

[Close]

BLAST of Lag0002671 vs. ExPASy Swiss-Prot
Match: Q84LK7 (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.7e-04
Identity = 24/46 (52.17%), Postives = 31/46 (67.39%), Query Frame = 0


Query: 63  SAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVG 109
           +A +  LT GNV+ VL+ VRPYL ADGG+V +  +   VV LKL G
Sbjct: 69  TAVQLPLTAGNVESVLDQVRPYLTADGGDVALHEIAGNVVRLKLQG 114

The following BLAST results are available for this feature:

BLAST of Lag0002671 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144330.1	1.2e-42	87.61	nifU-like protein 1, chloroplastic [Momordica charantia]	[more]
KAE8646369.1	3.7e-41	84.03	hypothetical protein Csa_016477 [Cucumis sativus]	[more]
XP_008451442.1	1.1e-40	85.84	PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo]	[more]
KAA0046713.1	1.1e-40	85.84	nifU-like protein 1 [Cucumis melo var. makuwa] >TYK18249.1 nifU-like protein 1 [...	[more]
XP_022991837.1	9.1e-40	82.30	nifU-like protein 1, chloroplastic [Cucurbita maxima]	[more]

BLAST of Lag0002671 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93W77	7.3e-16	62.65	NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1...	[more]
Q84LK7	1.7e-04	52.17	NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=N...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001075	NIF system FeS cluster assembly, NifU, C-terminal	PFAM	PF01106	NifU	coord: 75..108 e-value: 2.7E-8 score: 33.8
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 66..114 e-value: 2.8E-11 score: 45.4
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 68..110
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 32..108
None	No IPR available	PANTHER	PTHR11178:SF15	NIFU-LIKE PROTEIN 1, CHLOROPLASTIC	coord: 32..108

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002671.1	Lag0002671.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding