Lag0002652 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002652
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein kinase domain-containing protein
Locationchr4: 44528290 .. 44529225 (+)
RNA-Seq ExpressionLag0002652
SyntenyLag0002652
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAACTGGGTTCCATTGAAGATCTTAGGCGAAGGTTCTTACGGCGTAGTATTCTTAGCAAAACCAACAACCGGAACCACCCACCTCGACAACTTCTTCGCCATCAAAGCAACTCACCTTCAACACTACTCTTCACTCTCCAAAGAAAAACAGATTCTAAAACATTTCATCGGATGCCCCGAAATCGTCCAATGTTTCGGTAGCGAAATTACCATAGAAAAACACTCCCCCGACGACATGGCCTTTTACAATCTGAAACTGGAGTACGCCGCCGGCGGAACTCTAGCCGACCTGATCAAACAAAACCAGAAGCTACCGGAGAAGGAAGTGAAGCAGTACCTCAAAATGATCCTCAGAGGACTCTCCTGCATTCATCGCAAAGGGTTCGTCCACGTCGATCTCAAGCCCAGCAACATCCTCGCATTCCCTCAAACCGACGGCGAGACGAAGCTGAAGATCGCCGACTTCGGGCTTTCGAGGAGACGCGGCGAGGACGACGGCGGAGTTCCGACGTTTAAAGGGACGCCGAAATACATGTCGCCGGAATCGGTTATTTTCAGCGAGGTGAAGAGTTCGATGGATATCTGGTCGCTGGGCTGCATTTTGGCTGAAATGGTTTCGGGAAGATCGGTGTGGGATGATTGTTTGAGTCGCAGAGAGTTTGTTTCGAAACTTGTGTGTAGCGAGGAAATGCCGGAGATTCCTGAGGAATTGTCGGAAGAAGGGAAGGATTTCATTGGAAGATGCTTTGATCGGGACCATGAACGGCGGTGGACGGCGGATATGTTGCTCCGGCATCCGTATGTTAGAGAAGAAGAAAAGGAGGAAGGGATGAGAGGTAGAGTACTTCCGGGGAGACCAAAATTTCCGAAAGGGATTGATTTGCCTCATCTACAATGCAAGCTTCGATCGTGTATTCAAATTGCTTCATAA

mRNA sequence

ATGACGAACTGGGTTCCATTGAAGATCTTAGGCGAAGGTTCTTACGGCGTAGTATTCTTAGCAAAACCAACAACCGGAACCACCCACCTCGACAACTTCTTCGCCATCAAAGCAACTCACCTTCAACACTACTCTTCACTCTCCAAAGAAAAACAGATTCTAAAACATTTCATCGGATGCCCCGAAATCGTCCAATGTTTCGGTAGCGAAATTACCATAGAAAAACACTCCCCCGACGACATGGCCTTTTACAATCTGAAACTGGAGTACGCCGCCGGCGGAACTCTAGCCGACCTGATCAAACAAAACCAGAAGCTACCGGAGAAGGAAGTGAAGCAGTACCTCAAAATGATCCTCAGAGGACTCTCCTGCATTCATCGCAAAGGGTTCGTCCACGTCGATCTCAAGCCCAGCAACATCCTCGCATTCCCTCAAACCGACGGCGAGACGAAGCTGAAGATCGCCGACTTCGGGCTTTCGAGGAGACGCGGCGAGGACGACGGCGGAGTTCCGACGTTTAAAGGGACGCCGAAATACATGTCGCCGGAATCGGTTATTTTCAGCGAGGTGAAGAGTTCGATGGATATCTGGTCGCTGGGCTGCATTTTGGCTGAAATGGTTTCGGGAAGATCGGTGTGGGATGATTGTTTGAGTCGCAGAGAGTTTGTTTCGAAACTTGTGTGTAGCGAGGAAATGCCGGAGATTCCTGAGGAATTGTCGGAAGAAGGGAAGGATTTCATTGGAAGATGCTTTGATCGGGACCATGAACGGCGGTGGACGGCGGATATGTTGCTCCGGCATCCGTATGTTAGAGAAGAAGAAAAGGAGGAAGGGATGAGAGGTAGAGTACTTCCGGGGAGACCAAAATTTCCGAAAGGGATTGATTTGCCTCATCTACAATGCAAGCTTCGATCGTGTATTCAAATTGCTTCATAA

Coding sequence (CDS)

ATGACGAACTGGGTTCCATTGAAGATCTTAGGCGAAGGTTCTTACGGCGTAGTATTCTTAGCAAAACCAACAACCGGAACCACCCACCTCGACAACTTCTTCGCCATCAAAGCAACTCACCTTCAACACTACTCTTCACTCTCCAAAGAAAAACAGATTCTAAAACATTTCATCGGATGCCCCGAAATCGTCCAATGTTTCGGTAGCGAAATTACCATAGAAAAACACTCCCCCGACGACATGGCCTTTTACAATCTGAAACTGGAGTACGCCGCCGGCGGAACTCTAGCCGACCTGATCAAACAAAACCAGAAGCTACCGGAGAAGGAAGTGAAGCAGTACCTCAAAATGATCCTCAGAGGACTCTCCTGCATTCATCGCAAAGGGTTCGTCCACGTCGATCTCAAGCCCAGCAACATCCTCGCATTCCCTCAAACCGACGGCGAGACGAAGCTGAAGATCGCCGACTTCGGGCTTTCGAGGAGACGCGGCGAGGACGACGGCGGAGTTCCGACGTTTAAAGGGACGCCGAAATACATGTCGCCGGAATCGGTTATTTTCAGCGAGGTGAAGAGTTCGATGGATATCTGGTCGCTGGGCTGCATTTTGGCTGAAATGGTTTCGGGAAGATCGGTGTGGGATGATTGTTTGAGTCGCAGAGAGTTTGTTTCGAAACTTGTGTGTAGCGAGGAAATGCCGGAGATTCCTGAGGAATTGTCGGAAGAAGGGAAGGATTTCATTGGAAGATGCTTTGATCGGGACCATGAACGGCGGTGGACGGCGGATATGTTGCTCCGGCATCCGTATGTTAGAGAAGAAGAAAAGGAGGAAGGGATGAGAGGTAGAGTACTTCCGGGGAGACCAAAATTTCCGAAAGGGATTGATTTGCCTCATCTACAATGCAAGCTTCGATCGTGTATTCAAATTGCTTCATAA

Protein sequence

MTNWVPLKILGEGSYGVVFLAKPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQYLKMILRGLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRRRGEDDGGVPTFKGTPKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVCSEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEKEEGMRGRVLPGRPKFPKGIDLPHLQCKLRSCIQIAS
Homology
BLAST of Lag0002652 vs. NCBI nr
Match: XP_038896325.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 361.7 bits (927), Expect = 6.4e-96
Identity = 182/304 (59.87%), Postives = 224/304 (73.68%), Query Frame = 0

Query: 1   MTNWVPLKILGEGSYGVVFLAKP-TTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIG 60
           M NWV +KI+G GSYGVV LAK  T   +    +FA+K T LQ  SSL  E+Q+LKHF  
Sbjct: 1   MENWVLVKIIGRGSYGVVCLAKQMTKEKSDRHYYFAVKLTSLQRKSSLLWEEQVLKHFKD 60

Query: 61  CPEIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQYLKMIL 120
           CPEIVQC GSEIT     PDDM  YNLKLEYA GGTLADLIKQ +KLPE EVK YL+MIL
Sbjct: 61  CPEIVQCLGSEITF-GDIPDDMVLYNLKLEYAPGGTLADLIKQRKKLPEDEVKDYLRMIL 120

Query: 121 RGLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR-----RGEDDGGVPTFK 180
           +GLSCIHR+GFVHVDLKP+N+L FPQ+DG+ KLKIADFGL+ R       +D G +  FK
Sbjct: 121 KGLSCIHRRGFVHVDLKPANVLVFPQSDGKMKLKIADFGLAERCKHIVDDQDRGYLRKFK 180

Query: 181 GTPKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVCSEEMPE 240
           GTPKYMSPES+ F+EV  + DIWSLGCIL +MVSG+SVWDDC    + +++L+  + +P 
Sbjct: 181 GTPKYMSPESIFFNEVNGTHDIWSLGCILVKMVSGKSVWDDCRGSEQLMTRLLNDKNIPT 240

Query: 241 IPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEKEEGMRGRVLPGRPKFPKGI 299
           IP+ELS++GKDFI RCF R++++RWTADMLL+HPY+ EE +       ++    K  K I
Sbjct: 241 IPKELSKQGKDFIKRCFIRNYKQRWTADMLLQHPYLNEENEATKKGDDIV----KLIKAI 299

BLAST of Lag0002652 vs. NCBI nr
Match: XP_022150204.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 358.6 bits (919), Expect = 5.4e-95
Identity = 186/311 (59.81%), Postives = 230/311 (73.95%), Query Frame = 0

Query: 1   MTNWVPLKILGEGSYGVVFLAKPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIGC 60
           MTNWV +  LG+GSYG VFLAKPTT +     +FA+K   LQHYSSL  EK+ILKHF+G 
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSL---KYFAVKVAELQHYSSLLNEKRILKHFVGF 60

Query: 61  PEIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQYLKMILR 120
           PEIVQCFG E+  E  S  D AFY+L LEYAAGGTLADLI++ +KL E  VK+YLKMILR
Sbjct: 61  PEIVQCFGGEMITEGDS-FDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILR 120

Query: 121 GLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR--RGEDDGGVPTFKGTPK 180
           GLSCIH+ GFVHVDLKP+NILAFPQ DG+ KLKIADFGLSRR  +  +      F+GTP 
Sbjct: 121 GLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPM 180

Query: 181 YMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVCSEEMPEIPEE 240
           YMSPES I+ EV + +DIWSLGCIL EMV+G+  WDDC+   + + KL  + E P+IPEE
Sbjct: 181 YMSPES-IYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEE 240

Query: 241 LSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEKEEGMRGRVLPGRPKFPKGIDLPH 300
           LS+EGKDF+ +CFDR+ +RRWTADMLL+HPY+  E+++E ++      +   P    LPH
Sbjct: 241 LSKEGKDFLEKCFDRNPKRRWTADMLLQHPYL--EQEKEAIK------KDDDPTYSYLPH 298

Query: 301 LQCKLRSCIQI 310
           +  K +S IQI
Sbjct: 301 ILYKFQSTIQI 298

BLAST of Lag0002652 vs. NCBI nr
Match: XP_004136151.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 hypothetical protein Csa_015671 [Cucumis sativus])

HSP 1 Score: 342.8 bits (878), Expect = 3.1e-90
Identity = 182/311 (58.52%), Postives = 220/311 (70.74%), Query Frame = 0

Query: 3   NWVPLKILGEGSYGVVFLAKP-TTGTTHLDNFFAIKATHLQH-YSSLSKEKQILKHFIGC 62
           +WV +K LGEGS G+V LAK  T   + L  +FA+K   L++  SSL  E+ +LKHF  C
Sbjct: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63

Query: 63  PEIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQYLKMILR 122
           PEIVQ  GSE+T      DD   YNLKLEYAAGGTLADLIKQ  KLPE EVK+YL+MIL+
Sbjct: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMILK 123

Query: 123 GLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR---RGEDD---------G 182
           GLSCIHRKGFVHVDLKP NILAFPQ+DG+ KLKIADFGL+ R   RGEDD          
Sbjct: 124 GLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYYS 183

Query: 183 GVPTFKGTPKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVC 242
           G    + T +YMSPES++FSE+    DIWSLGC L +MVSG  VW+DC S  E ++KL+ 
Sbjct: 184 GALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLLI 243

Query: 243 SEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEKEEGMRGRVLPGRP 299
           SEE+P IPEELS++GKDF+ +CF R++E+RWTADMLL+HPY+ EE K+       L    
Sbjct: 244 SEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKL---- 303

BLAST of Lag0002652 vs. NCBI nr
Match: KAA0059705.1 (mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. makuwa] >TYK26143.1 mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. makuwa])

HSP 1 Score: 336.7 bits (862), Expect = 2.2e-88
Identity = 171/291 (58.76%), Postives = 216/291 (74.23%), Query Frame = 0

Query: 1   MTNWVPLKILGEGSYGVVFLAKPTT-----GTTHLDNFFAIKATHLQHYSSLSKEKQILK 60
           M NWVP+K+LG+GSYGVV LAK ++       ++L  +FA+K + LQH SSL  E+Q+LK
Sbjct: 1   MENWVPVKVLGQGSYGVVCLAKQSSAIRKKSDSNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  HFIGCPEIVQCFGSEITIEKHSPDD-MAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQY 120
            F  CPEIVQ  GSEIT  +   +D + FY LKLEYAAGGTL DLI+Q  KLPE EVK Y
Sbjct: 61  QFKDCPEIVQYLGSEITRGRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEVKDY 120

Query: 121 LKMILRGLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR---RGEDDG--- 180
           L+MIL+GLSCIH KGFVHVDLKP+NILAFPQ+DG+ KLKIADFG + R   R +D     
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQY 180

Query: 181 ----GVPTFKGTPKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVS 240
                   FKGTP+YMSPESV+F+EV  + DIWSLGCIL +M+SG+S WDDC   ++ + 
Sbjct: 181 PPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMI 240

Query: 241 KLVCSEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEK 276
           KL+ ++ MP IP ELSE+GKDFI +CF R+++ RWTA+MLL+HPY+ EE +
Sbjct: 241 KLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENE 291

BLAST of Lag0002652 vs. NCBI nr
Match: XP_008465601.1 (PREDICTED: mitogen-activated protein kinase kinase kinase ANP1-like, partial [Cucumis melo])

HSP 1 Score: 334.0 bits (855), Expect = 1.4e-87
Identity = 170/291 (58.42%), Postives = 214/291 (73.54%), Query Frame = 0

Query: 1   MTNWVPLKILGEGSYGVVFLAKPTT-----GTTHLDNFFAIKATHLQHYSSLSKEKQILK 60
           M NWVP+K+LG+GSY VV LAK ++        +L  +FA+K + LQH SSL  E+Q+LK
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSSAIRKKSDNNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  HFIGCPEIVQCFGSEITIEKHSPDD-MAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQY 120
            F  CPEIVQ  GSEIT  +   +D + FY LKLEYAAGGTL DLI+Q  KLPE EVK Y
Sbjct: 61  QFKDCPEIVQYLGSEITRGRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEVKDY 120

Query: 121 LKMILRGLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR---RGEDDG--- 180
           L+MIL+GLSCIH KGFVHVDLKP+NILAFPQ+DG+ KLKIADFG + R   R +D     
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQY 180

Query: 181 ----GVPTFKGTPKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVS 240
                   FKGTP+YMSPESV+F+EV  + DIWSLGCIL +M+SG+S WDDC   ++ + 
Sbjct: 181 PPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMI 240

Query: 241 KLVCSEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEK 276
           KL+ ++ MP IP ELSE+GKDFI +CF R+++ RWTA+MLL+HPY+ EE +
Sbjct: 241 KLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENE 291

BLAST of Lag0002652 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 7.2e-50
Identity = 112/275 (40.73%), Postives = 163/275 (59.27%), Query Frame = 0

Query: 4   WVPLKILGEGSYGVVFLAKPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEI 63
           WV  + +G G++  V  A  +  +       A+K+T     +SLS EK +L     CPEI
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEI 62

Query: 64  VQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQ--NQKLPEKEVKQYLKMILRG 123
           ++C+G + T+E    +    +NL LEYA+ G+LA  +K+   + LPE  V+++   +LRG
Sbjct: 63  IRCYGEDSTVE----NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRG 122

Query: 124 LSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRRRGEDDGGV---PTFKGTPK 183
           L  IH KGF H D+K +NIL F   DG   +KIADFGL+ R   D   +      +GTP 
Sbjct: 123 LRHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMRVDGDLTALRKSVEIRGTPL 182

Query: 184 YMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLV---CSEEMPEI 243
           YM+PE V  +E  S+ D+W+LGC + EM SG++ W        F+S L+     +E+P+I
Sbjct: 183 YMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAW-SVKEGSHFMSLLIRIGVGDELPKI 242

Query: 244 PEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYV 271
           PE LSEEGKDF+ +CF +D  +RWTA+MLL H +V
Sbjct: 243 PEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFV 268

BLAST of Lag0002652 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.3e-37
Identity = 100/271 (36.90%), Postives = 140/271 (51.66%), Query Frame = 0

Query: 4   WVPLKILGEGSYGVVFLAKPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEI 63
           W   +ILG GS   V+ A       + D   A+K++ +     L +E +IL   +  P +
Sbjct: 3   WTRGRILGRGSTATVYAA----AGHNSDEILAVKSSEVHRSEFLQREAKILSS-LSSPYV 62

Query: 64  VQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLAD-LIKQNQKLPEKEVKQYLKMILRGL 123
           +   GSE    K   + +  YNL +EYA  GTL D   K   ++ E  V +Y + IL+GL
Sbjct: 63  IGYRGSE---TKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGL 122

Query: 124 SCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRRRGEDDGGVPTFKGTPKYMSP 183
             IH KG VH D+K SN++      GE   KIADFG ++R   D        GTP +M+P
Sbjct: 123 EYIHSKGIVHCDVKGSNVVI--SEKGEA--KIADFGCAKR--VDPVFESPVMGTPAFMAP 182

Query: 184 ESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLV---CSEEMPEIPEEL 243
           E     +     DIW++GC + EMV+G   W    SR + VS L     S E PE+P  L
Sbjct: 183 EVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLL 242

Query: 244 SEEGKDFIGRCFDRDHERRWTADMLLRHPYV 271
           +EE KDF+ +C  R+   RWTA  LL HP++
Sbjct: 243 AEEAKDFLEKCLKREANERWTATQLLNHPFL 259

BLAST of Lag0002652 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-33
Identity = 84/269 (31.23%), Postives = 137/269 (50.93%), Query Frame = 0

Query: 4   WVPLKILGEGSYGVVFLA-KPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPE 63
           W   +ILG G YG V+L     TG       FA+K   +   +S  K K ++  F    E
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTG-----ELFAVKQLEIVDINSDPKLKNMILSFSKEIE 229

Query: 64  IVQCFGSEITIEKHSPD-DMAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQYLKMILRG 123
           +++    +  +       D +F ++ LEY  GG+++ L+ +     E  +K Y K IL+G
Sbjct: 230 VMRSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQG 289

Query: 124 LSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRRRGEDDGGVPTFKGTPKYMS 183
           LS +H    +H D+K +NIL     D +  +K++DFG S+          + +GTP +M+
Sbjct: 290 LSFLHANSIIHRDIKGANIL----IDTKGIVKLSDFGCSKSFSGIVSQFKSMQGTPYWMA 349

Query: 184 PESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVCSEEMPEIPEELSE 243
           PE +  +    S DIWSLGC++ EM + +  W +       +  +  S  +P IP  +S+
Sbjct: 350 PEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQ 409

Query: 244 EGKDFIGRCFDRDHERRWTADMLLRHPYV 271
           E  DF+  CF RD + R  A+ LL+HP++
Sbjct: 410 EAFDFLNLCFKRDPKERPDANQLLKHPFI 429

BLAST of Lag0002652 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-33
Identity = 96/281 (34.16%), Postives = 141/281 (50.18%), Query Frame = 0

Query: 3   NWVPLKILGEGSYGVVFLAKPTTGTTH-LDNFFAIKATHLQHYSSLSKEKQILKHFIGCP 62
           NW   K LG GS   V     +  T H      A+K+        L +E +IL   +  P
Sbjct: 2   NWTRGKTLGRGSTATV-----SAATCHESGETLAVKSAEFHRSEFLQREAKILSS-LNSP 61

Query: 63  EIVQCFGSEITIEK-HSPDDMAFYNLKLEYAAGGTLADLIKQNQK-LPEKEVKQYLKMIL 122
            ++   G EIT E  H+  +   Y+L +EYA  GTL D+  +N   + E  V +Y + IL
Sbjct: 62  YVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQIL 121

Query: 123 RGLSCIHR-KGFVHVDLKPSNILAFPQTDGET-KLKIADFGLSRRRGEDDGGVPTFKGTP 182
            GL  IH  KG  H D+K SN+L      GE  + KIADFG +  +  +       +GTP
Sbjct: 122 LGLEYIHNSKGIAHCDIKGSNVLV-----GENGEAKIADFGCA--KWVEPEITEPVRGTP 181

Query: 183 KYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVW--DDCLSRREFVSKLVCSEEMPEI 242
            +M+PE+          DIW++GC + EMV+G   W   D       + ++    E+PE+
Sbjct: 182 AFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPEL 241

Query: 243 PEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEKE 277
           P  L+E+ KDF+G+C  ++   RWTA  LL HP++  +E E
Sbjct: 242 PCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLVNKEPE 269

BLAST of Lag0002652 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.0e-32
Identity = 95/282 (33.69%), Postives = 152/282 (53.90%), Query Frame = 0

Query: 2   TNWVPLKILGEGSYGVVFLAKPTTGTTHLDNFFAIKATHL--------QHYSSLSKEKQI 61
           + W   ++LG GS+G V+L       +      A+K   L        +    L +E  +
Sbjct: 398 SRWKKGRLLGMGSFGHVYLG----FNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISV 457

Query: 62  LKHFIGCPEIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQ 121
           L   +    IVQ +GSE      + DD  +  + LEY +GG++  L+++  +  E  ++ 
Sbjct: 458 LSR-LRHQNIVQYYGSE------TVDDKLY--IYLEYVSGGSIYKLLQEYGQFGENAIRN 517

Query: 122 YLKMILRGLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRRRGEDDGGVPTF 181
           Y + IL GL+ +H K  VH D+K +NIL     D   ++K+ADFG+++      G + +F
Sbjct: 518 YTQQILSGLAYLHAKNTVHRDIKGANIL----VDPHGRVKVADFGMAKHITAQSGPL-SF 577

Query: 182 KGTPKYMSPESVIFSEVKS-SMDIWSLGCILAEMVSGRSVWDDCLSRREFVS---KLVCS 241
           KG+P +M+PE +  S   + ++DIWSLGC + EM + +  W    S+ E V    K+  S
Sbjct: 578 KGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPW----SQYEGVPAMFKIGNS 637

Query: 242 EEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVR 272
           +E+P+IP+ LSEEGKDF+ +C  R+   R TA  LL H +VR
Sbjct: 638 KELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFVR 657

BLAST of Lag0002652 vs. ExPASy TrEMBL
Match: A0A6J1D9A6 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111018431 PE=4 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 2.6e-95
Identity = 186/311 (59.81%), Postives = 230/311 (73.95%), Query Frame = 0

Query: 1   MTNWVPLKILGEGSYGVVFLAKPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIGC 60
           MTNWV +  LG+GSYG VFLAKPTT +     +FA+K   LQHYSSL  EK+ILKHF+G 
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSL---KYFAVKVAELQHYSSLLNEKRILKHFVGF 60

Query: 61  PEIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQYLKMILR 120
           PEIVQCFG E+  E  S  D AFY+L LEYAAGGTLADLI++ +KL E  VK+YLKMILR
Sbjct: 61  PEIVQCFGGEMITEGDS-FDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILR 120

Query: 121 GLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR--RGEDDGGVPTFKGTPK 180
           GLSCIH+ GFVHVDLKP+NILAFPQ DG+ KLKIADFGLSRR  +  +      F+GTP 
Sbjct: 121 GLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPM 180

Query: 181 YMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVCSEEMPEIPEE 240
           YMSPES I+ EV + +DIWSLGCIL EMV+G+  WDDC+   + + KL  + E P+IPEE
Sbjct: 181 YMSPES-IYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEE 240

Query: 241 LSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEKEEGMRGRVLPGRPKFPKGIDLPH 300
           LS+EGKDF+ +CFDR+ +RRWTADMLL+HPY+  E+++E ++      +   P    LPH
Sbjct: 241 LSKEGKDFLEKCFDRNPKRRWTADMLLQHPYL--EQEKEAIK------KDDDPTYSYLPH 298

Query: 301 LQCKLRSCIQI 310
           +  K +S IQI
Sbjct: 301 ILYKFQSTIQI 298

BLAST of Lag0002652 vs. ExPASy TrEMBL
Match: A0A0A0K835 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407720 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 1.5e-90
Identity = 182/311 (58.52%), Postives = 220/311 (70.74%), Query Frame = 0

Query: 3   NWVPLKILGEGSYGVVFLAKP-TTGTTHLDNFFAIKATHLQH-YSSLSKEKQILKHFIGC 62
           +WV +K LGEGS G+V LAK  T   + L  +FA+K   L++  SSL  E+ +LKHF  C
Sbjct: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63

Query: 63  PEIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQYLKMILR 122
           PEIVQ  GSE+T      DD   YNLKLEYAAGGTLADLIKQ  KLPE EVK+YL+MIL+
Sbjct: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMILK 123

Query: 123 GLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR---RGEDD---------G 182
           GLSCIHRKGFVHVDLKP NILAFPQ+DG+ KLKIADFGL+ R   RGEDD          
Sbjct: 124 GLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYYS 183

Query: 183 GVPTFKGTPKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVC 242
           G    + T +YMSPES++FSE+    DIWSLGC L +MVSG  VW+DC S  E ++KL+ 
Sbjct: 184 GALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLLI 243

Query: 243 SEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEKEEGMRGRVLPGRP 299
           SEE+P IPEELS++GKDF+ +CF R++E+RWTADMLL+HPY+ EE K+       L    
Sbjct: 244 SEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKL---- 303

BLAST of Lag0002652 vs. ExPASy TrEMBL
Match: A0A5D3DR57 (Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00640 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 1.1e-88
Identity = 171/291 (58.76%), Postives = 216/291 (74.23%), Query Frame = 0

Query: 1   MTNWVPLKILGEGSYGVVFLAKPTT-----GTTHLDNFFAIKATHLQHYSSLSKEKQILK 60
           M NWVP+K+LG+GSYGVV LAK ++       ++L  +FA+K + LQH SSL  E+Q+LK
Sbjct: 1   MENWVPVKVLGQGSYGVVCLAKQSSAIRKKSDSNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  HFIGCPEIVQCFGSEITIEKHSPDD-MAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQY 120
            F  CPEIVQ  GSEIT  +   +D + FY LKLEYAAGGTL DLI+Q  KLPE EVK Y
Sbjct: 61  QFKDCPEIVQYLGSEITRGRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEVKDY 120

Query: 121 LKMILRGLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR---RGEDDG--- 180
           L+MIL+GLSCIH KGFVHVDLKP+NILAFPQ+DG+ KLKIADFG + R   R +D     
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQY 180

Query: 181 ----GVPTFKGTPKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVS 240
                   FKGTP+YMSPESV+F+EV  + DIWSLGCIL +M+SG+S WDDC   ++ + 
Sbjct: 181 PPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMI 240

Query: 241 KLVCSEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEK 276
           KL+ ++ MP IP ELSE+GKDFI +CF R+++ RWTA+MLL+HPY+ EE +
Sbjct: 241 KLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENE 291

BLAST of Lag0002652 vs. ExPASy TrEMBL
Match: A0A1S3CQQ0 (mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656 GN=LOC103503238 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 6.9e-88
Identity = 170/291 (58.42%), Postives = 214/291 (73.54%), Query Frame = 0

Query: 1   MTNWVPLKILGEGSYGVVFLAKPTT-----GTTHLDNFFAIKATHLQHYSSLSKEKQILK 60
           M NWVP+K+LG+GSY VV LAK ++        +L  +FA+K + LQH SSL  E+Q+LK
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSSAIRKKSDNNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  HFIGCPEIVQCFGSEITIEKHSPDD-MAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQY 120
            F  CPEIVQ  GSEIT  +   +D + FY LKLEYAAGGTL DLI+Q  KLPE EVK Y
Sbjct: 61  QFKDCPEIVQYLGSEITRGRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEVKDY 120

Query: 121 LKMILRGLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR---RGEDDG--- 180
           L+MIL+GLSCIH KGFVHVDLKP+NILAFPQ+DG+ KLKIADFG + R   R +D     
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQY 180

Query: 181 ----GVPTFKGTPKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVS 240
                   FKGTP+YMSPESV+F+EV  + DIWSLGCIL +M+SG+S WDDC   ++ + 
Sbjct: 181 PPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMI 240

Query: 241 KLVCSEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEK 276
           KL+ ++ MP IP ELSE+GKDFI +CF R+++ RWTA+MLL+HPY+ EE +
Sbjct: 241 KLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENE 291

BLAST of Lag0002652 vs. ExPASy TrEMBL
Match: A0A5A7TZP4 (Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001750 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 3.4e-87
Identity = 173/310 (55.81%), Postives = 218/310 (70.32%), Query Frame = 0

Query: 1   MTNWVPLKILGEGSYGVVFLAKP-TTGTTHLDNFFAIKATHLQHYS-SLSKEKQILKHFI 60
           M +WV LK+LGEGS G+V LAK  T   + L  +FA+K   L++ S SL  E+ +LKH  
Sbjct: 1   MEDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLT 60

Query: 61  GCPEIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQNQKLPEKEVKQYLKMI 120
            CPEIVQ  GSE+T      DD   YNLKLEYAAGGTLADLIKQ  KLPE EVK+YL+MI
Sbjct: 61  DCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMI 120

Query: 121 LRGLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRR--RGEDDG-------- 180
           L+GLSCIHRKGFVHVDLKP NILAFPQ+DG+ KLKIADFGL+ R   GEDD         
Sbjct: 121 LKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERFKHGEDDQEDKGCEHY 180

Query: 181 GVPTFKGTPKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVC 240
           G    + T +YMSPES++  E+    DIWSLGC+L +MVSG  VW+DC +  E ++KL+ 
Sbjct: 181 GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLLN 240

Query: 241 SEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEKEEGMRGRVLPGRP 299
           S+E+P IP+ELS++G DF+ +CF RD+++RWTADMLL+HPY+ E+ K+     ++     
Sbjct: 241 SKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNKDTSDDEKL----- 300

BLAST of Lag0002652 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-52
Identity = 122/296 (41.22%), Postives = 176/296 (59.46%), Query Frame = 0

Query: 4   WVPLKILGEGSYGVVFLAKPTTGTTH--LDNFFAIKATHLQHYSSLSKEKQILKHFIGCP 63
           W+  + +G GS+  V LA  T+G++     +  A+K++ +   ++L  E+ +L     C 
Sbjct: 3   WIRRETIGHGSFSTVSLA-TTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCS 62

Query: 64  EIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQN-QKLPEKEVKQYLKMILR 123
           EIV+CFG   T+E    +    YNL LEYA+GG+LAD IK + + LPE EV+++ + I++
Sbjct: 63  EIVRCFGEGRTVE----NGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVK 122

Query: 124 GLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRRRGEDDGGVPTFKGTPKYM 183
           GL  IH  GF H D+K  N+L F    G+  +KI+DFGL++RR  +       +GTP YM
Sbjct: 123 GLCHIHGNGFTHCDIKLENVLVF----GDGDVKISDFGLAKRRSGE--VCVEIRGTPLYM 182

Query: 184 SPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLS---RREFVSKLV---CSEEMPE 243
           +PESV   E +S  DIW+LGC + EM SG++ W  CL        +S LV     +E+P 
Sbjct: 183 APESVNHGEFESPADIWALGCSVVEMSSGKTAW--CLEDGVMNNVMSLLVRIGSGDEVPR 242

Query: 244 IPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPY--VREEEKEEGMRGRVLPGRP 289
           IP ELSEEGKDF+ +CF ++   RWTA+MLL HP+  V +E  EE     V P  P
Sbjct: 243 IPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEEDEACSVSPRNP 285

BLAST of Lag0002652 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 199.1 bits (505), Expect = 5.1e-51
Identity = 112/275 (40.73%), Postives = 163/275 (59.27%), Query Frame = 0

Query: 4   WVPLKILGEGSYGVVFLAKPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEI 63
           WV  + +G G++  V  A  +  +       A+K+T     +SLS EK +L     CPEI
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEI 62

Query: 64  VQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQ--NQKLPEKEVKQYLKMILRG 123
           ++C+G + T+E    +    +NL LEYA+ G+LA  +K+   + LPE  V+++   +LRG
Sbjct: 63  IRCYGEDSTVE----NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRG 122

Query: 124 LSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRRRGEDDGGV---PTFKGTPK 183
           L  IH KGF H D+K +NIL F   DG   +KIADFGL+ R   D   +      +GTP 
Sbjct: 123 LRHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMRVDGDLTALRKSVEIRGTPL 182

Query: 184 YMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLV---CSEEMPEI 243
           YM+PE V  +E  S+ D+W+LGC + EM SG++ W        F+S L+     +E+P+I
Sbjct: 183 YMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAW-SVKEGSHFMSLLIRIGVGDELPKI 242

Query: 244 PEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYV 271
           PE LSEEGKDF+ +CF +D  +RWTA+MLL H +V
Sbjct: 243 PEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFV 268

BLAST of Lag0002652 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 192.6 bits (488), Expect = 4.8e-49
Identity = 111/277 (40.07%), Postives = 166/277 (59.93%), Query Frame = 0

Query: 4   WVPLKILGEGSYGVVFLA-KPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHF-IGCP 63
           W+  + +G G++  V LA +    +       A+K+      +SL+ EK +L +    C 
Sbjct: 3   WIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCN 62

Query: 64  EIVQCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQ--NQKLPEKEVKQYLKMIL 123
           EIV+CFG + T+E    +    +NL LEYA+ G+L   +K+   + +PE  V+++   +L
Sbjct: 63  EIVRCFGEDRTVE----NGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVL 122

Query: 124 RGLSCIHRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRRRGEDDG---GVPTFKGT 183
           RGL  IH  GF H DLK  NIL F    G+  +KIADFGL++R G+      GV   +GT
Sbjct: 123 RGLRHIHANGFAHCDLKLGNILLF----GDGAVKIADFGLAKRIGDLTALNYGV-QIRGT 182

Query: 184 PKYMSPESVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLV---CSEEMP 243
           P YM+PESV  +E  S  D+W+LGC++ EM SG++ W        F+S L+     +E+P
Sbjct: 183 PLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAW-SLKEGSNFMSLLLRIGVGDEVP 242

Query: 244 EIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYV 271
            IPEELSE+G+DF+ +CF +D ++RWTA+MLL HP+V
Sbjct: 243 MIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFV 269

BLAST of Lag0002652 vs. TAIR 10
Match: AT2G05060.1 (Protein kinase superfamily protein )

HSP 1 Score: 188.7 bits (478), Expect = 6.9e-48
Identity = 104/268 (38.81%), Postives = 159/268 (59.33%), Query Frame = 0

Query: 8   KILGEGSYGVVFLAKPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEIVQCF 67
           K+LG+GS G V L K  +       + A+K +++ H  SL KE QIL  F GC  IVQC+
Sbjct: 18  KVLGKGSSGSVSLIKYKSRLDGQTLYAAVKTSNIIHADSLLKEFQILSEFKGCSRIVQCY 77

Query: 68  GSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIK--QNQKLPEKEVKQYLKMILRGLSCI 127
           G+++    +   D+ F  + +EYA+GG+L   +   ++ KLP+  ++++ +MIL GL+ I
Sbjct: 78  GTKVQETINEEGDVEF-TIPMEYASGGSLRHFMSRFKDMKLPDALIRRFTRMILEGLAVI 137

Query: 128 HRKGFVHVDLKPSNILAFPQTDGETKLKIADFGLSRRRGEDDGGVPT--FKGTPKYMSPE 187
           H  G+VH DLKP NIL FP  +    LKI+DFGLS+R G+    +P+  F GTP YMSPE
Sbjct: 138 HGHGYVHCDLKPENILVFPSFE----LKISDFGLSKREGDSKWWLPSHPFAGTPVYMSPE 197

Query: 188 SVIFSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVCSEEMPEIPEELSEEG 247
           S+   E +  +D+WSLGC++ EM +G+  W D    + +    +    MP I +++  + 
Sbjct: 198 SISNGETRRGLDLWSLGCVVLEMYTGKRPWWD----KNYDLGDLKKGSMPLISKDIPCDA 257

Query: 248 KDFIGRCFDRDHERRWTADMLLRHPYVR 272
           K F+  CF  +  +R  A  LLRH ++R
Sbjct: 258 KLFVMTCFASETNKRKNAFTLLRHCFLR 276

BLAST of Lag0002652 vs. TAIR 10
Match: AT2G41930.1 (Protein kinase superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-47
Identity = 120/316 (37.97%), Postives = 179/316 (56.65%), Query Frame = 0

Query: 7   LKILGEGSYGVV--FLAKPTTGTTHLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEIV 66
           +K+LG+G+YG V  F  K   G+     + A+K    ++Y S+ +E +IL    GCP IV
Sbjct: 8   VKVLGKGTYGSVELFSHKQNDGSL---LYNAVKIMDSENYGSIDQEFRILSELRGCPCIV 67

Query: 67  QCFGSEITIEKHSPDDMAFYNLKLEYAAGGTLADLIKQNQ-KLPEKEVKQYLKMILRGLS 126
           Q  G+ + ++    +    Y + +EYAA GTL + IK+N+ KL +  +K + +MIL+GL 
Sbjct: 68  QLCGNSL-VQGIDCNGKKVYMMSMEYAAAGTLTNFIKRNRTKLSDSVIKDFTRMILQGLV 127

Query: 127 CIHRKGFVHVDLKPSNILAFPQTDGET-----KLKIADFGLSRRRGEDDG---GVPTFKG 186
            IH  G+VH DLKP NIL FP  D +T     +LKI+DFG+S R G+  G       + G
Sbjct: 128 SIHNHGYVHCDLKPDNILLFPLYDKDTWNCSYELKISDFGISTRAGDKSGCWRVDEPWVG 187

Query: 187 TPKYMSPESVI-FSEVKSSMDIWSLGCILAEMVSGRSVWDDCLSRREFVSKLVCSEEMPE 246
           T  YMSPESV   + V+ ++D+WSLGCI+ +M +G+  W   L   + V  L+ +++ PE
Sbjct: 188 TSIYMSPESVSDGTTVEKTLDLWSLGCIVLKMYTGKRPW---LGFEKDVKSLLLNQKAPE 247

Query: 247 IPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEEKEEG------MRGRVLPGRP 305
           IPE L  + + F+ +CF R  E R +A  LL HP++  +EK+          G VL  R 
Sbjct: 248 IPETLPCDARLFLEKCFSRKPEERGSASELLLHPFLTGDEKKGSSVAGGERTGMVLRLRK 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896325.16.4e-9659.87mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida][more]
XP_022150204.15.4e-9559.81mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia][more]
XP_004136151.13.1e-9058.52mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 ... [more]
KAA0059705.12.2e-8858.76mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. maku... [more]
XP_008465601.11.4e-8758.42PREDICTED: mitogen-activated protein kinase kinase kinase ANP1-like, partial [Cu... [more]
Match NameE-valueIdentityDescription
Q9SND67.2e-5040.73Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808885.3e-3736.90Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q54R822.1e-3331.23Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Q9ZVP52.7e-3334.16Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q9CAD54.0e-3233.69Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1D9A62.6e-9559.81mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
A0A0A0K8351.5e-9058.52Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407... [more]
A0A5D3DR571.1e-8858.76Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. ma... [more]
A0A1S3CQQ06.9e-8858.42mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656... [more]
A0A5A7TZP43.4e-8755.81Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
AT4G36950.11.2e-5241.22mitogen-activated protein kinase kinase kinase 21 [more]
AT3G50310.15.1e-5140.73mitogen-activated protein kinase kinase kinase 20 [more]
AT5G67080.14.8e-4940.07mitogen-activated protein kinase kinase kinase 19 [more]
AT2G05060.16.9e-4838.81Protein kinase superfamily protein [more]
AT2G41930.11.2e-4737.97Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 4..270
e-value: 2.3E-66
score: 236.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 7..270
e-value: 8.6E-54
score: 182.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 4..270
score: 40.385799
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 1..284
e-value: 6.0E-64
score: 218.3
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 7..216
e-value: 3.3E-8
score: 31.0
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..276
NoneNo IPR availablePANTHERPTHR48011:SF51PROTEIN KINASE SUPERFAMILY PROTEINcoord: 1..276
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 10..37
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002652.1Lag0002652.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds
molecular_function GO:0004672 protein kinase activity