Lag0002649 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002649
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	calvin cycle protein CP12-1, chloroplastic-like
Location	chr4: 44517131 .. 44517508 (+)
RNA-Seq Expression	Lag0002649
Synteny	Lag0002649

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCAATCTCCGGCGCGACCACGGCGGCCTCAAAAGCGGTGGCTGACTCTCCGAAACCCGAGTCGCCGGTCCCCCGATCCGCCAGGCTCAGCTACCCATGGAAGCGTTGGACCGCCGGTCGAATGATCGCGGTTGGACCGGTGTCTGCCGCACCGGACCGGATATCGGAGAAGGTTGTTGAGAGCATAAAGAACGCGGAGGAGACGTGCTCGGAGGATCCGGCGAGCGGCGAGTGCGCGGCTGCGTGGGATGAGGTGGAGGAGCTGAGCGCCGCCGCTAGCCACGCACGTGACCGGCTGAAGGATGCGGACCCGCTTGAGGATTTTTGTAAGGATAATCCGGAGACGGAGGAGTGTCGCACGTATGAGGACTGA

mRNA sequence

ATGGCAGCAATCTCCGGCGCGACCACGGCGGCCTCAAAAGCGGTGGCTGACTCTCCGAAACCCGAGTCGCCGGTCCCCCGATCCGCCAGGCTCAGCTACCCATGGAAGCGTTGGACCGCCGGTCGAATGATCGCGGTTGGACCGGTGTCTGCCGCACCGGACCGGATATCGGAGAAGGTTGTTGAGAGCATAAAGAACGCGGAGGAGACGTGCTCGGAGGATCCGGCGAGCGGCGAGTGCGCGGCTGCGTGGGATGAGGTGGAGGAGCTGAGCGCCGCCGCTAGCCACGCACGTGACCGGCTGAAGGATGCGGACCCGCTTGAGGATTTTTGTAAGGATAATCCGGAGACGGAGGAGTGTCGCACGTATGAGGACTGA

Coding sequence (CDS)

ATGGCAGCAATCTCCGGCGCGACCACGGCGGCCTCAAAAGCGGTGGCTGACTCTCCGAAACCCGAGTCGCCGGTCCCCCGATCCGCCAGGCTCAGCTACCCATGGAAGCGTTGGACCGCCGGTCGAATGATCGCGGTTGGACCGGTGTCTGCCGCACCGGACCGGATATCGGAGAAGGTTGTTGAGAGCATAAAGAACGCGGAGGAGACGTGCTCGGAGGATCCGGCGAGCGGCGAGTGCGCGGCTGCGTGGGATGAGGTGGAGGAGCTGAGCGCCGCCGCTAGCCACGCACGTGACCGGCTGAAGGATGCGGACCCGCTTGAGGATTTTTGTAAGGATAATCCGGAGACGGAGGAGTGTCGCACGTATGAGGACTGA

Protein sequence

MAAISGATTAASKAVADSPKPESPVPRSARLSYPWKRWTAGRMIAVGPVSAAPDRISEKVVESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDADPLEDFCKDNPETEECRTYED

Homology

[Close]

BLAST of Lag0002649 vs. NCBI nr
Match: XP_022991513.1 (calvin cycle protein CP12-1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 221.5 bits (563), Expect = 4.1e-54
Identity = 115/125 (92.00%), Postives = 119/125 (95.20%), Query Frame = 0


Query: 1   MAAISGATTAASKAVADSPKPESPVPRSARLSYPWKRWTAGRMIAVGPVSAAPDRISEKV 60
           MAAISGAT A SKAVADSPK ESPV RSARL+YPWKRW+A R+ AVGPVSAAPDRISEKV
Sbjct: 1   MAAISGATMAVSKAVADSPKLESPVLRSARLNYPWKRWSAVRLCAVGPVSAAPDRISEKV 60

Query: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDADPLEDFCKDNPETEEC 120
           VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKD+DPLEDFCKDNPETEEC
Sbjct: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEEC 120

Query: 121 RTYED 126
           RTYED
Sbjct: 121 RTYED 125

[Close]

BLAST of Lag0002649 vs. NCBI nr
Match: KAG6575763.1 (Calvin cycle protein CP12-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 219.5 bits (558), Expect = 1.6e-53
Identity = 115/125 (92.00%), Postives = 118/125 (94.40%), Query Frame = 0


Query: 1   MAAISGATTAASKAVADSPKPESPVPRSARLSYPWKRWTAGRMIAVGPVSAAPDRISEKV 60
           MAAISGAT A SKAVADSPK ESPV RSARL YPWKRW+A R+ AVGPVSAAPDRISEKV
Sbjct: 1   MAAISGATMAVSKAVADSPKLESPVLRSARLYYPWKRWSAVRLRAVGPVSAAPDRISEKV 60

Query: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDADPLEDFCKDNPETEEC 120
           VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKD+DPLEDFCKDNPETEEC
Sbjct: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEEC 120

Query: 121 RTYED 126
           RTYED
Sbjct: 121 RTYED 125

[Close]

BLAST of Lag0002649 vs. NCBI nr
Match: XP_023548709.1 (calvin cycle protein CP12-1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 217.2 bits (552), Expect = 7.8e-53
Identity = 113/125 (90.40%), Postives = 117/125 (93.60%), Query Frame = 0


Query: 1   MAAISGATTAASKAVADSPKPESPVPRSARLSYPWKRWTAGRMIAVGPVSAAPDRISEKV 60
           MAAISGAT   SKAVADSP  ESPV RSARL+YPWKRW+A R+ AVGPVSAAPDRISEKV
Sbjct: 1   MAAISGATMGVSKAVADSPNLESPVLRSARLNYPWKRWSAVRLRAVGPVSAAPDRISEKV 60

Query: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDADPLEDFCKDNPETEEC 120
           VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKD+DPLEDFCKDNPETEEC
Sbjct: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEEC 120

Query: 121 RTYED 126
           RTYED
Sbjct: 121 RTYED 125

[Close]

BLAST of Lag0002649 vs. NCBI nr
Match: XP_022953775.1 (calvin cycle protein CP12-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 216.5 bits (550), Expect = 1.3e-52
Identity = 114/125 (91.20%), Postives = 117/125 (93.60%), Query Frame = 0


Query: 1   MAAISGATTAASKAVADSPKPESPVPRSARLSYPWKRWTAGRMIAVGPVSAAPDRISEKV 60
           MAAISGAT A SKAVADSPK ES V RSARL YPWKRW+A R+ AVGPVSAAPDRISEKV
Sbjct: 1   MAAISGATMAVSKAVADSPKLESTVLRSARLYYPWKRWSAVRLRAVGPVSAAPDRISEKV 60

Query: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDADPLEDFCKDNPETEEC 120
           VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKD+DPLEDFCKDNPETEEC
Sbjct: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEEC 120

Query: 121 RTYED 126
           RTYED
Sbjct: 121 RTYED 125

[Close]

BLAST of Lag0002649 vs. NCBI nr
Match: KAG7014316.1 (Calvin cycle protein CP12-1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 214.5 bits (545), Expect = 5.1e-52
Identity = 112/122 (91.80%), Postives = 116/122 (95.08%), Query Frame = 0


Query: 1   MAAISGATTAASKAVADSPKPESPVPRSARLSYPWKRWTAGRMIAVGPVSAAPDRISEKV 60
           MAAISGAT A SKAVADSPK ESPV RSARL+YPWKRW+A R+ AVGPVSAAPDRISEKV
Sbjct: 1   MAAISGATMAVSKAVADSPKLESPVLRSARLNYPWKRWSAVRLRAVGPVSAAPDRISEKV 60

Query: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDADPLEDFCKDNPETEEC 120
           VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKD+DPLEDFCKDNPETEEC
Sbjct: 61  VESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEEC 120

Query: 121 RT 123
           RT
Sbjct: 121 RT 122

[Close]

BLAST of Lag0002649 vs. ExPASy Swiss-Prot
Match: O22914 (Calvin cycle protein CP12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP12-1 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.4e-25
Identity = 67/125 (53.60%), Postives = 86/125 (68.80%), Query Frame = 0


Query: 3   AISGATTAASKAVADSPKPESPVPRSARLSYPWKRWTAGRMIAVGPVSAAPDRISEKVVE 62
           A +G   A  + V    +P + V    RL+YPWK  +  RM+ V   S     ISEKV +
Sbjct: 5   AAAGLNVATPRVVV---RPVARVLGPVRLNYPWKFGSMKRMVVVKATSEG--EISEKVEK 64

Query: 63  SIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLK--DADPLEDFCKDNPETEEC 122
           SI+ A+ETC++DP SGEC AAWDEVEELSAAASHARD+ K   +DPLE++C DNPET+EC
Sbjct: 65  SIQEAKETCADDPVSGECVAAWDEVEELSAAASHARDKKKAGGSDPLEEYCNDNPETDEC 124

Query: 123 RTYED 126
           RTY++
Sbjct: 125 RTYDN 124

[Close]

BLAST of Lag0002649 vs. ExPASy Swiss-Prot
Match: Q9LZP9 (Calvin cycle protein CP12-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP12-2 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 4.6e-24
Identity = 58/90 (64.44%), Postives = 72/90 (80.00%), Query Frame = 0


Query: 39  TAGRMIAVGPVSAAPD-RISEKVVESIKNAEETCSEDPASGECAAAWDEVEELSAAASHA 98
           T  RM+ + P+ AAP+  IS+ V +SIK A+ETC+ DP SGEC AAWDEVEELSAAASHA
Sbjct: 42  TTNRMMKLQPIKAAPEGGISDVVEKSIKEAQETCAGDPVSGECVAAWDEVEELSAAASHA 101

Query: 99  RDRLK--DADPLEDFCKDNPETEECRTYED 126
           RD+ K   +DPLE++CKDNPET ECRTY++
Sbjct: 102 RDKKKADGSDPLEEYCKDNPETNECRTYDN 131

[Close]

BLAST of Lag0002649 vs. ExPASy Swiss-Prot
Match: A6Q0K5 (Calvin cycle protein CP12, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=CP12 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 6.3e-13
Identity = 46/104 (44.23%), Postives = 63/104 (60.58%), Query Frame = 0


Query: 27  RSARLSYPWKRWTAGRMIAVGPVSAAPD-RISEKVVESIKNAEETCSEDPASGECAAAWD 86
           +S  +S P  R  + R   V   S  P   +++KV +++K AE+ C++   S +CA AWD
Sbjct: 5   KSVVISRPAVRPVSTRRAVVVRASGQPAVDLNKKVQDAVKEAEDACAKG-TSADCAVAWD 64

Query: 87  EVEELSAAASHARDRLKD----ADPLEDFCKDNPETEECRTYED 126
            VEELSAA SH +D +K      DPLE FCKD P+ +ECR YED
Sbjct: 65  TVEELSAAVSHKKDAVKADVTLTDPLEAFCKDAPDADECRVYED 107

[Close]

BLAST of Lag0002649 vs. ExPASy Swiss-Prot
Match: Q9C9K2 (Calvin cycle protein CP12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP12-3 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 2.4e-12
Identity = 51/135 (37.78%), Postives = 74/135 (54.81%), Query Frame = 0


Query: 4   ISGATTAA-SKAVADSPKPESPVPRSARLSYPWKRWTAGRMIAVGPVSAA---------- 63
           ISG+ TA+  + +  S +   PV   + +     R T  R  ++  V+ A          
Sbjct: 2   ISGSATASHGRVLLPSQRERRPVSTGSNILR--FRETVPRQFSLMMVTKATAKYMGTKMR 61

Query: 64  PDRISEKVVESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLK--DADPLEDF 123
            +++SE + E +K A E C  +  S EC  AWDEVEE+S A +  R +LK  + DPLE F
Sbjct: 62  EEKLSEMIEEKVKEATEVCEAEEMSEECRVAWDEVEEVSQARADLRIKLKLLNQDPLESF 121

Query: 124 CKDNPETEECRTYED 126
           C++NPET+ECR YED
Sbjct: 122 CQENPETDECRIYED 134

The following BLAST results are available for this feature:

BLAST of Lag0002649 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022991513.1	4.1e-54	92.00	calvin cycle protein CP12-1, chloroplastic-like [Cucurbita maxima]	[more]
KAG6575763.1	1.6e-53	92.00	Calvin cycle protein CP12-1, chloroplastic, partial [Cucurbita argyrosperma subs...	[more]
XP_023548709.1	7.8e-53	90.40	calvin cycle protein CP12-1, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022953775.1	1.3e-52	91.20	calvin cycle protein CP12-1, chloroplastic-like [Cucurbita moschata]	[more]
KAG7014316.1	5.1e-52	91.80	Calvin cycle protein CP12-1, chloroplastic, partial [Cucurbita argyrosperma subs...	[more]

BLAST of Lag0002649 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22914	1.4e-25	53.60	Calvin cycle protein CP12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP...	[more]
Q9LZP9	4.6e-24	64.44	Calvin cycle protein CP12-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP...	[more]
A6Q0K5	6.3e-13	44.23	Calvin cycle protein CP12, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN...	[more]
Q9C9K2	2.4e-12	37.78	Calvin cycle protein CP12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003823	Domain of unknown function CP12	SMART	SM01093	CP12_2	coord: 56..125 e-value: 1.3E-27 score: 107.7
IPR003823	Domain of unknown function CP12	PFAM	PF02672	CP12	coord: 56..124 e-value: 3.9E-26 score: 91.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 95..125
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..30
None	No IPR available	PANTHER	PTHR33921:SF15	CALVIN CYCLE PROTEIN CP12-2, CHLOROPLASTIC	coord: 1..125
IPR039314	Calvin cycle protein CP12-like	PANTHER	PTHR33921	CALVIN CYCLE PROTEIN CP12-2, CHLOROPLASTIC	coord: 1..125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002649.1	Lag0002649.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity