Lag0002605 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002605 vs. NCBI nr
Match: XP_008451544.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KAA0046659.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa] >TYK18194.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa])

HSP 1 Score: 1021.5 bits (2640), Expect = 2.7e-294
Identity = 512/563 (90.94%), Postives = 535/563 (95.03%), Query Frame = 0

Query: 1   MAAGPTTSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60
           MAAG T + + +++LI LY+TTS +     PTGSVCSSTPDPSYCKSALPNQTGNVYSYG
Sbjct: 1   MAAGTTAAAFYVLILISLYITTSAS-----PTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60

Query: 61  RSSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTT 120
           RSS RKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQ+LAGLN+DYL TSF+ VN T
Sbjct: 61  RSSFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNAT 120

Query: 121 SRTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAL 180
           SR L EMKAD+VQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLA 
Sbjct: 121 SRALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDD--GGDD 240
           FTKGWVPKKKK+PTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRR+LLQTDD  G DD
Sbjct: 181 FTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDD 240

Query: 241 QISVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRY 300
           QI+VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYV IAKNKRY
Sbjct: 241 QIAVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRY 300

Query: 301 LMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVR 360
           LMMIGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+R
Sbjct: 301 LMMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALR 360

Query: 361 NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 420
           NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM
Sbjct: 361 NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 420

Query: 421 PNQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQ 480
            NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANN+ A VKTFLGRPWKEYSRTVY+Q
Sbjct: 421 SNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQ 480

Query: 481 SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTA 540
           SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERV WPGFHLIND+DAGNFTA
Sbjct: 481 SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTA 540

Query: 541 ANFVLADDWLPQTGVPYTSGLTE 562
            NFVLADDWLPQTGVPYTSGLTE
Sbjct: 541 GNFVLADDWLPQTGVPYTSGLTE 558

BLAST of Lag0002605 vs. NCBI nr
Match: XP_038896831.1 (probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida])

HSP 1 Score: 1020.0 bits (2636), Expect = 7.8e-294
Identity = 512/560 (91.43%), Postives = 531/560 (94.82%), Query Frame = 0

Query: 2   AAGPTTSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 61
           AA    +FY LI LI LY+TTS A     PTGS CSST DPSYCKS LPNQTGNVYSYGR
Sbjct: 8   AATAAAAFYFLI-LISLYITTSAA-----PTGSECSSTRDPSYCKSVLPNQTGNVYSYGR 67

Query: 62  SSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTTS 121
           SS RKSLS+SQKFLRLVEKHLRSRSSLTV AVRALEDCQ+LAGLN+DYLTTSFR VN+TS
Sbjct: 68  SSFRKSLSASQKFLRLVEKHLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTS 127

Query: 122 RTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLALF 181
           RTLPEMKAD+VQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLAADTKLYS+SLA F
Sbjct: 128 RTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFF 187

Query: 182 TKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDDGGDDQIS 241
           TKGWVPKKKK+PTWKAAGR GGFRNGRMSLKMSSR +AIYETATRR+LLQ DDGGDDQI+
Sbjct: 188 TKGWVPKKKKKPTWKAAGRHGGFRNGRMSLKMSSRAKAIYETATRRNLLQADDGGDDQIA 247

Query: 242 VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMM 301
           VRDIVVVSQDG+GNFTTINEA+AAAPNNSAPTDGYFLIFVSAGVYEEYV IAKNKRYLMM
Sbjct: 248 VRDIVVVSQDGSGNFTTINEAVAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM 307

Query: 302 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 361
           IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA
Sbjct: 308 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 367

Query: 362 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQ 421
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM NQ
Sbjct: 368 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 427

Query: 422 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFM 481
           FNAITAQGRTDPNQNTGTSI+NCRITAADDLANNT A VKTFLGRPWKEYSRTVY+QSFM
Sbjct: 428 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNTGAGVKTFLGRPWKEYSRTVYMQSFM 487

Query: 482 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTAANF 541
           DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERV WPGFHLIND+DAGNFTA NF
Sbjct: 488 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 547

Query: 542 VLADDWLPQTGVPYTSGLTE 562
           VLADDWLPQTGVPYTSGLT+
Sbjct: 548 VLADDWLPQTGVPYTSGLTD 561

BLAST of Lag0002605 vs. NCBI nr
Match: XP_004136013.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1 hypothetical protein Csa_016133 [Cucumis sativus])

HSP 1 Score: 1016.9 bits (2628), Expect = 6.6e-293
Identity = 514/565 (90.97%), Postives = 534/565 (94.51%), Query Frame = 0

Query: 1   MAAGPT---TSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVY 60
           MAAG T    +FY L++LI LY+TTS +     PTGSVCSSTPDPSYCKSALPNQTGNVY
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSAS-----PTGSVCSSTPDPSYCKSALPNQTGNVY 60

Query: 61  SYGRSSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTV 120
           SYGRSS RKSLSSSQKFLRLVEKHLRSRSSLTV AVRALEDC +LAGLN+DYL TSF+TV
Sbjct: 61  SYGRSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTV 120

Query: 121 NTTSRTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMS 180
           NTTSR L EMKAD+VQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+S
Sbjct: 121 NTTSRVLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLS 180

Query: 181 LALFTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDDGG- 240
           LA FTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYE ATRR+LLQTDDGG 
Sbjct: 181 LAFFTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGD 240

Query: 241 DDQISVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNK 300
           DDQI VRDIVVVSQDG+GNFTTINEAIAAA NNSAPTDGYFLIFVSAGVYEEYVL+AKNK
Sbjct: 241 DDQIKVRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNK 300

Query: 301 RYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA 360
           RYLMMIGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA
Sbjct: 301 RYLMMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA 360

Query: 361 VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL 420
           VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL
Sbjct: 361 VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL 420

Query: 421 PMPNQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVY 480
           PM NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANN+ A VKTFLGRPWKEYSRTVY
Sbjct: 421 PMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVY 480

Query: 481 LQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNF 540
           +QSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTW GFHLIND+DAGNF
Sbjct: 481 MQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNF 540

Query: 541 TAANFVLADDWLPQTGVPYTSGLTE 562
           TA NFVLADDWLPQTGVPY SGLTE
Sbjct: 541 TAGNFVLADDWLPQTGVPYDSGLTE 560

BLAST of Lag0002605 vs. NCBI nr
Match: KAG7014346.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1010.7 bits (2612), Expect = 4.7e-291
Identity = 504/560 (90.00%), Postives = 530/560 (94.64%), Query Frame = 0

Query: 2   AAGPTTSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 61
           AA PTT+ +  +LLI LY+T +  +    PTGSVCSS PDPSYCKSALPNQTGNVYSYGR
Sbjct: 3   AAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGR 62

Query: 62  SSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTTS 121
           SSVRKSLSSSQKFLRLVEK+LRSRSSLTVAAVRALEDCQ+LAGLN+DYLTTSFRTVN+TS
Sbjct: 63  SSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTS 122

Query: 122 RTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLALF 181
            TLPEMKA++VQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA F
Sbjct: 123 WTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFF 182

Query: 182 TKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDDGGDDQIS 241
           TKGWVPKKKK+PTW +AGRQGGF NGRMSLKMSSRT+AIYETATRR+LLQTDDGGDDQIS
Sbjct: 183 TKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQIS 242

Query: 242 VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMM 301
           VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV IAKNKRYLMM
Sbjct: 243 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMM 302

Query: 302 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 361
           IGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN A
Sbjct: 303 IGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSA 362

Query: 362 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQ 421
           DLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM NQ
Sbjct: 363 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQ 422

Query: 422 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFM 481
           FNAITAQGRTDPNQNTGTSIHNCRITAADDLANN+ A V TFLGRPWKEYSRTVY+QSFM
Sbjct: 423 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFM 482

Query: 482 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTAANF 541
           DDLINPAGWRAWDGDFALNT YYAEFGNFGPGSNT+ERV W GFHLI+D+DAGNFTA NF
Sbjct: 483 DDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNF 542

Query: 542 VLADDWLPQTGVPYTSGLTE 562
           ++ADDW+PQTGVPYTSGLTE
Sbjct: 543 LMADDWVPQTGVPYTSGLTE 562

BLAST of Lag0002605 vs. NCBI nr
Match: KAG6575804.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1010.4 bits (2611), Expect = 6.2e-291
Identity = 504/560 (90.00%), Postives = 530/560 (94.64%), Query Frame = 0

Query: 2   AAGPTTSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 61
           AA PTT+ +  +LLI LY+T +  +    PTGSVCSS PDPSYCKSALPNQTGNVYSYGR
Sbjct: 3   AAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGR 62

Query: 62  SSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTTS 121
           SSVRKSLSSSQKFLRLVEK+LRSRSSLTVAAVRALEDCQ+LAGLN+DYLTTSFRTVN+TS
Sbjct: 63  SSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTS 122

Query: 122 RTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLALF 181
            TLPEMKA++VQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA F
Sbjct: 123 WTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFF 182

Query: 182 TKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDDGGDDQIS 241
           TKGWVPKKKK+PTW +AGRQGGF NGRMSLKMSSRT+AIYETATRR+LLQT+DGGDDQIS
Sbjct: 183 TKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQIS 242

Query: 242 VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMM 301
           VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV IAKNKRYLMM
Sbjct: 243 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMM 302

Query: 302 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 361
           IGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN A
Sbjct: 303 IGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSA 362

Query: 362 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQ 421
           DLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM NQ
Sbjct: 363 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQ 422

Query: 422 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFM 481
           FNAITAQGRTDPNQNTGTSIHNCRITAADDLANN+ A V TFLGRPWKEYSRTVY+QSFM
Sbjct: 423 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFM 482

Query: 482 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTAANF 541
           DDLINPAGWRAWDGDFALNT YYAEFGNFGPGSNTSERV W GFHLI+D+DAGNFTA NF
Sbjct: 483 DDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAGNF 542

Query: 542 VLADDWLPQTGVPYTSGLTE 562
           ++ADDW+PQTGVPYTSGLTE
Sbjct: 543 LMADDWVPQTGVPYTSGLTE 562

BLAST of Lag0002605 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 4.7e-201
Identity = 354/573 (61.78%), Postives = 433/573 (75.57%), Query Frame = 0

Query: 10  YSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSVRKSLS 69
           + LI L F   +   ++     + ++C +TPDP YCKS  P+  GNV  YG  S+RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 70  SSQKFLRLVEKHLRSRSSLT-VAAVRALEDCQVLAGLNMDYLTTSFRTVNTTS-----RT 129
            S+KF+R V+++++  + L+  A +RAL+DC+ LAGL MDYL TSF TVN TS     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 130 LPEMKADEVQSLLSAILTNQQTCLDGLKATA---GSWSLKNGLSLPLAADTKLYSMSLAL 189
           L   KAD++Q+LLSA LTN+QTCL+GL   A    +W+++ G++LPL  DTKL  +SLAL
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 190 FTKGWVPKKKKRPTWKAAGRQGG----------FRNGRMSLKMSSRTQAIYETATRRHLL 249
           FTKGWVPKKKKR  +  A  + G          FRNG + LKM+ +T+A+YE+ +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 250 QTDDGGD----DQISVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVY 309
             D  GD      + + DIV VSQDGTGNFT I  A+AAAPNN+  + G+FLI+V+AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 310 EEYVLIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNT 369
           EEY+ IAKNKRY+MMIGDGINQTVVTGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 370 AGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 429
           AG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 430 FQNCNIYPRLPMPNQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGR 489
           FQNCN+YPR PMPNQFNAITAQGR+DPNQNTGTSI NC I  ADDL ++    VKT+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN-YTVKTYLGR 485

Query: 490 PWKEYSRTVYLQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFH 549
           PWKEYSRTVY+QS++D  + P GWR W+GDFAL+T YYAE+ N GPGSNT+ RVTWPG+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 550 LINDSDAGNFTAANFVLADDWLPQTGVPYTSGL 560
           +IN +DA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of Lag0002605 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 686.4 bits (1770), Expect = 2.7e-196
Identity = 352/566 (62.19%), Postives = 438/566 (77.39%), Query Frame = 0

Query: 13  ILLIFLYLTT----STATPPPPPTGSVCSSTPDPSYCKSALPNQT-GNVYSYGRSSVRKS 72
           ++ +FL L T    S    P   + ++C +TPDP +CKS  P  + G+V  YGR S+RKS
Sbjct: 8   LVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKS 67

Query: 73  LSSSQKFLRLVEKHL-RSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVN--TTSRTL 132
           L+ S+KF R ++++L R+ + L+ +AV AL+DC+ LA L  DYL TSF TVN  T+S+TL
Sbjct: 68  LTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 127

Query: 133 PEMKADEVQSLLSAILTNQQTCLDGLK-ATAGSWSLKNGLSLPLAADTKLYSMSLALFTK 192
              KADE+Q+LLSA LTN+QTCLDG+  A + SW+++NG++LPL  DTKL+S+SLALFTK
Sbjct: 128 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTK 187

Query: 193 GWVPKKKKRP---TWKAAGRQGG-------FRNGRMSLKMSSRTQAIYETATRRHLLQTD 252
           GWVPKKKK+    +W               FRNG + LKM+  T+A+YE+ +RR L   D
Sbjct: 188 GWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-D 247

Query: 253 DGGDDQISVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIA 312
           D   + + V DIV V+Q+GTGNFTTI EA+ +APN +  T GYF+I+V++GVYEE V+IA
Sbjct: 248 DNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIA 307

Query: 313 KNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQ 372
           KNKRYLMMIGDGIN+TVVTGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQ
Sbjct: 308 KNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQ 367

Query: 373 AVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIY 432
           AVA+R+ ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+Y
Sbjct: 368 AVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLY 427

Query: 433 PRLPMPNQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSR 492
           PR PM NQFNAITAQGRTDPNQNTG SIHNC I  ADDL ++    VKT+LGRPWKEYSR
Sbjct: 428 PRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSN-YTVKTYLGRPWKEYSR 487

Query: 493 TVYLQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDA 552
           TV++QS++D+++ P GWR W+GDFAL+T YYAE+ N G GS+T++RV WPG+H+IN +DA
Sbjct: 488 TVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDA 547

Query: 553 GNFTAANFVLADDWLPQTGVPYTSGL 560
            NFT  NF+L D W+ Q+GVPY SGL
Sbjct: 548 NNFTVENFLLGDGWMVQSGVPYISGL 571

BLAST of Lag0002605 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 661.0 bits (1704), Expect = 1.2e-188
Identity = 326/531 (61.39%), Postives = 412/531 (77.59%), Query Frame = 0

Query: 34  SVCSSTPDPSYCKSALPNQTGNVYSYGRSSVRKSLSSSQKFLRLVEKHLRSRSSLTV-AA 93
           ++C   PDPSYC+S LPNQ G++YSYGR S+R+SLS +++F+ +++  L  +  +   + 
Sbjct: 37  NICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKST 96

Query: 94  VRALEDCQVLAGLNMDYLTTSFRTVNTTSRTLPEMKADEVQSLLSAILTNQQTCLDGLKA 153
           V ALEDC+ LA L MDYL +S +T ++T +TL   +A++V + LSA +TN+QTCL+GLK+
Sbjct: 97  VGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTCLEGLKS 156

Query: 154 TAGSWSLKNGLSLPLAADTKLYSMSLALFTKGWVPKKKK-RPTWKAAGRQG---GFRNGR 213
           TA     +NGLS  L  DTKLY +SLALF+KGWVP++++ RP W+   R     GFRNG+
Sbjct: 157 TAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGK 216

Query: 214 MSLKMSSRTQAIYETATRRHLLQTDDGGDDQISVRDIVVVSQDGTGNFTTINEAIAAAPN 273
           + LKM+ R +A+Y T TRR LLQ+D    D + V DIV V Q+GTGNFTTIN AIAAAPN
Sbjct: 217 LPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAAIAAAPN 276

Query: 274 NSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSAT 333
            +  ++GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+TGNRSVVDGWTTFNSAT
Sbjct: 277 KTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSAT 336

Query: 334 FAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYR 393
           F + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHSLRQFYR
Sbjct: 337 FILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYR 396

Query: 394 DCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQFNAITAQGRTDPNQNTGTSIHNCRITA 453
           +CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+IH C I  
Sbjct: 397 ECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRP 456

Query: 454 ADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFMDDLINPAGWRAWDGDFALNTSYYAEFG 513
           ADDLA +    VKT+LGRPWKEYSRTV +Q+++D  + P+GW AW GDFAL+T YYAE+ 
Sbjct: 457 ADDLATSN-YTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYN 516

Query: 514 NFGPGSNTSERVTWPGFHLINDSDAGNFTAANFVLADDWLPQTGVPYTSGL 560
           N GPGS+T+ RVTWPG+H+IN +DA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 517 NTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of Lag0002605 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.9e-136
Identity = 264/552 (47.83%), Postives = 352/552 (63.77%), Query Frame = 0

Query: 23  STATPPPPPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSVRKSLSSSQKFLRLVE 82
           S + P P P    C STP P  C+   SA+ +   + Y YG+ ++++ L  +++  +++ 
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 83  KHLR----SRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTTSRTLPEMKADEVQSL 142
           +  +       + TV  V A+ DC  LA L+++YL T    +   +  +     D V SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVTSL 194

Query: 143 LSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLALFTKGWVPKKKKRPTW 202
           L  ++TNQQTCLDGL      ++    +  PL   T+LYS+SL L +       K+    
Sbjct: 195 LGGVVTNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGS 254

Query: 203 KAAGRQGGFRNGRMSLKMSSRTQAIYETATR--------RHLLQTDDGGDDQISVRDIVV 262
           K  G+  G  N  +   + +  + + +T  +        R+L +  +     I VR+ V 
Sbjct: 255 K--GKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVT 314

Query: 263 VSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGIN 322
           V    T NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV+I+  KR +M+IGDGIN
Sbjct: 315 VGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGIN 374

Query: 323 QTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFY 382
           +T+++GN S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY
Sbjct: 375 KTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFY 434

Query: 383 LCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQFNAITA 442
            CSFE YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM NQ NA+TA
Sbjct: 435 RCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTA 494

Query: 443 QGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFMDDLINP 502
            GRTDPNQ TG SI NC I AA DLA +  + + TFLGRPWK YSRTVY+QS++ D++ P
Sbjct: 495 HGRTDPNQKTGISIINCTIGAAPDLAADPKSTM-TFLGRPWKPYSRTVYIQSYISDVVQP 554

Query: 503 AGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTAANFVLADDW 560
            GW  W+G   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D W
Sbjct: 555 VGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTW 614

BLAST of Lag0002605 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 4.5e-135
Identity = 260/553 (47.02%), Postives = 354/553 (64.01%), Query Frame = 0

Query: 27  PPPPPTGS-----VCSSTPDPSYCK---SALPNQTGNVYSYGRSSVRKSLSSSQKFLRLV 86
           P  PP+ S      C STP P  C+   +A+ +   + Y YG+ ++++ L  + +  +++
Sbjct: 69  PSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 128

Query: 87  EKHLR----SRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTTSRTLPEMKADEVQS 146
             + R       S T   + A+ DC  L+ L+++YL T   T   T++ +     + V S
Sbjct: 129 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLET-VTTELKTAQVMTAALVEHVNS 188

Query: 147 LLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLALFTKGWVPKKKKRPT 206
           LLS ++TNQQTCLDGL      ++    +  P+   T+LYS+SL L +       K+   
Sbjct: 189 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKA 248

Query: 207 WKAAGRQGGFRNGRMSLKMSSRTQAIYETA--------TRRHLLQTDDGGDDQISVRDIV 266
            K     GG  N      + +  + + +T         T R+L +  +     I V   V
Sbjct: 249 SKGKILGGG--NSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAV 308

Query: 267 VVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGI 326
           +V    + NFTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+++  NK+ LM++GDGI
Sbjct: 309 IVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGI 368

Query: 327 NQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTF 386
           N+T++TGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+F
Sbjct: 369 NKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSF 428

Query: 387 YLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQFNAIT 446
           Y CSFE YQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM  Q NAIT
Sbjct: 429 YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAIT 488

Query: 447 AQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFMDDLIN 506
           A GR DPNQNTG SI NC I AA DLA    +A+ TFLGRPWK YSRTV++QS++ D++ 
Sbjct: 489 AHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQ 548

Query: 507 PAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTAANFVLADD 560
           P GW  W+G   L+T YY E+ NFGPG+NT++RV W G++L+N ++A NFT  NF + D 
Sbjct: 549 PVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDT 608

BLAST of Lag0002605 vs. ExPASy TrEMBL
Match: A0A5D3D3H4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001320 PE=3 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.3e-294
Identity = 512/563 (90.94%), Postives = 535/563 (95.03%), Query Frame = 0

Query: 1   MAAGPTTSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60
           MAAG T + + +++LI LY+TTS +     PTGSVCSSTPDPSYCKSALPNQTGNVYSYG
Sbjct: 1   MAAGTTAAAFYVLILISLYITTSAS-----PTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60

Query: 61  RSSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTT 120
           RSS RKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQ+LAGLN+DYL TSF+ VN T
Sbjct: 61  RSSFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNAT 120

Query: 121 SRTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAL 180
           SR L EMKAD+VQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLA 
Sbjct: 121 SRALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDD--GGDD 240
           FTKGWVPKKKK+PTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRR+LLQTDD  G DD
Sbjct: 181 FTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDD 240

Query: 241 QISVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRY 300
           QI+VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYV IAKNKRY
Sbjct: 241 QIAVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRY 300

Query: 301 LMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVR 360
           LMMIGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+R
Sbjct: 301 LMMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALR 360

Query: 361 NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 420
           NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM
Sbjct: 361 NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 420

Query: 421 PNQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQ 480
            NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANN+ A VKTFLGRPWKEYSRTVY+Q
Sbjct: 421 SNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQ 480

Query: 481 SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTA 540
           SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERV WPGFHLIND+DAGNFTA
Sbjct: 481 SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTA 540

Query: 541 ANFVLADDWLPQTGVPYTSGLTE 562
            NFVLADDWLPQTGVPYTSGLTE
Sbjct: 541 GNFVLADDWLPQTGVPYTSGLTE 558

BLAST of Lag0002605 vs. ExPASy TrEMBL
Match: A0A1S3BSI2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.3e-294
Identity = 512/563 (90.94%), Postives = 535/563 (95.03%), Query Frame = 0

Query: 1   MAAGPTTSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60
           MAAG T + + +++LI LY+TTS +     PTGSVCSSTPDPSYCKSALPNQTGNVYSYG
Sbjct: 1   MAAGTTAAAFYVLILISLYITTSAS-----PTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60

Query: 61  RSSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTT 120
           RSS RKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQ+LAGLN+DYL TSF+ VN T
Sbjct: 61  RSSFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNAT 120

Query: 121 SRTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAL 180
           SR L EMKAD+VQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLA 
Sbjct: 121 SRALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDD--GGDD 240
           FTKGWVPKKKK+PTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRR+LLQTDD  G DD
Sbjct: 181 FTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDD 240

Query: 241 QISVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRY 300
           QI+VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYV IAKNKRY
Sbjct: 241 QIAVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRY 300

Query: 301 LMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVR 360
           LMMIGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+R
Sbjct: 301 LMMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALR 360

Query: 361 NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 420
           NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM
Sbjct: 361 NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 420

Query: 421 PNQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQ 480
            NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANN+ A VKTFLGRPWKEYSRTVY+Q
Sbjct: 421 SNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQ 480

Query: 481 SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTA 540
           SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERV WPGFHLIND+DAGNFTA
Sbjct: 481 SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTA 540

Query: 541 ANFVLADDWLPQTGVPYTSGLTE 562
            NFVLADDWLPQTGVPYTSGLTE
Sbjct: 541 GNFVLADDWLPQTGVPYTSGLTE 558

BLAST of Lag0002605 vs. ExPASy TrEMBL
Match: A0A0A0KB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 3.2e-293
Identity = 514/565 (90.97%), Postives = 534/565 (94.51%), Query Frame = 0

Query: 1   MAAGPT---TSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVY 60
           MAAG T    +FY L++LI LY+TTS +     PTGSVCSSTPDPSYCKSALPNQTGNVY
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSAS-----PTGSVCSSTPDPSYCKSALPNQTGNVY 60

Query: 61  SYGRSSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTV 120
           SYGRSS RKSLSSSQKFLRLVEKHLRSRSSLTV AVRALEDC +LAGLN+DYL TSF+TV
Sbjct: 61  SYGRSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTV 120

Query: 121 NTTSRTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMS 180
           NTTSR L EMKAD+VQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+S
Sbjct: 121 NTTSRVLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLS 180

Query: 181 LALFTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDDGG- 240
           LA FTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYE ATRR+LLQTDDGG 
Sbjct: 181 LAFFTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGD 240

Query: 241 DDQISVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNK 300
           DDQI VRDIVVVSQDG+GNFTTINEAIAAA NNSAPTDGYFLIFVSAGVYEEYVL+AKNK
Sbjct: 241 DDQIKVRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNK 300

Query: 301 RYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA 360
           RYLMMIGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA
Sbjct: 301 RYLMMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA 360

Query: 361 VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL 420
           VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL
Sbjct: 361 VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL 420

Query: 421 PMPNQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVY 480
           PM NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANN+ A VKTFLGRPWKEYSRTVY
Sbjct: 421 PMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVY 480

Query: 481 LQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNF 540
           +QSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTW GFHLIND+DAGNF
Sbjct: 481 MQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNF 540

Query: 541 TAANFVLADDWLPQTGVPYTSGLTE 562
           TA NFVLADDWLPQTGVPY SGLTE
Sbjct: 541 TAGNFVLADDWLPQTGVPYDSGLTE 560

BLAST of Lag0002605 vs. ExPASy TrEMBL
Match: A0A6J1GQE6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 8.7e-291
Identity = 503/560 (89.82%), Postives = 529/560 (94.46%), Query Frame = 0

Query: 2   AAGPTTSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 61
           AA PTT+ +  +LLI LY+T +  +    PTGSVCSS PDPSYCKSALPNQTGNVYSYGR
Sbjct: 3   AAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGR 62

Query: 62  SSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTTS 121
           SSVRKSLSSSQKFLRLVEK+LRSRSSLTVAAVRALEDCQ+LAGLN+DYLTTSFRTVN+TS
Sbjct: 63  SSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTS 122

Query: 122 RTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLALF 181
            TLPEMKA++VQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA F
Sbjct: 123 WTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFF 182

Query: 182 TKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDDGGDDQIS 241
           TKGWVPKKKK+PTW +AGRQGGF NGRMSLKMSSRT+AIYETATRR+LLQT+DGGDDQIS
Sbjct: 183 TKGWVPKKKKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQIS 242

Query: 242 VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMM 301
           VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV IAKNKRYLMM
Sbjct: 243 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMM 302

Query: 302 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 361
           IGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRN AGAIKHQAVAVRN A
Sbjct: 303 IGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNAAGAIKHQAVAVRNSA 362

Query: 362 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQ 421
           DLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM NQ
Sbjct: 363 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQ 422

Query: 422 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFM 481
           FNAITAQGRTDPNQNTGTSIHNCRITAADDLANN+ A V TFLGRPWKEYSRTVY+QSFM
Sbjct: 423 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFM 482

Query: 482 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTAANF 541
           DDLINPAGWRAWDGDFALNT YYAEFGNFGPGSNTSERV W GFHLI+D+DAGNFTA NF
Sbjct: 483 DDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAGNF 542

Query: 542 VLADDWLPQTGVPYTSGLTE 562
           ++ADDW+PQTGVPYTSGLTE
Sbjct: 543 LMADDWVPQTGVPYTSGLTE 562

BLAST of Lag0002605 vs. ExPASy TrEMBL
Match: A0A6J1JVJ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.8e-289
Identity = 501/560 (89.46%), Postives = 526/560 (93.93%), Query Frame = 0

Query: 2   AAGPTTSFYSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 61
           AA PTT  +  +LLI LY+T +  +    PTGSVCSS PDPSYCKSALPNQTGNVYSYGR
Sbjct: 3   AAPPTTPAFYYLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGR 62

Query: 62  SSVRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTTS 121
           SSVRKSLSSSQKFLRLVEK+LRSRSSLTVAAVR+LEDCQ+LAGLN+DYLTTSFRTVN+TS
Sbjct: 63  SSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRTVNSTS 122

Query: 122 RTLPEMKADEVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLALF 181
            TLPEMKA++VQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPL ADTKLYSMSLA F
Sbjct: 123 WTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSMSLAFF 182

Query: 182 TKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRHLLQTDDGGDDQIS 241
           TKGWVPKKKK+ TW +AGRQGGF NGRMS KMSSRT+AIYE ATRR+LLQTDDGGDDQIS
Sbjct: 183 TKGWVPKKKKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQTDDGGDDQIS 242

Query: 242 VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMM 301
           VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV IAKNKRYLMM
Sbjct: 243 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMM 302

Query: 302 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 361
           IGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN A
Sbjct: 303 IGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSA 362

Query: 362 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQ 421
           DLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM NQ
Sbjct: 363 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQ 422

Query: 422 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFM 481
           FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNT A V TFLGRPWKEYSRTVY+QSFM
Sbjct: 423 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGARVNTFLGRPWKEYSRTVYMQSFM 482

Query: 482 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTAANF 541
           +DLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERV W GFHLI+D+DAGNFTA NF
Sbjct: 483 NDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAGNF 542

Query: 542 VLADDWLPQTGVPYTSGLTE 562
           ++ADDW+PQTGVPYTSGLTE
Sbjct: 543 LMADDWVPQTGVPYTSGLTE 562

BLAST of Lag0002605 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 702.2 bits (1811), Expect = 3.4e-202
Identity = 354/573 (61.78%), Postives = 433/573 (75.57%), Query Frame = 0

Query: 10  YSLILLIFLYLTTSTATPPPPPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSVRKSLS 69
           + LI L F   +   ++     + ++C +TPDP YCKS  P+  GNV  YG  S+RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 70  SSQKFLRLVEKHLRSRSSLT-VAAVRALEDCQVLAGLNMDYLTTSFRTVNTTS-----RT 129
            S+KF+R V+++++  + L+  A +RAL+DC+ LAGL MDYL TSF TVN TS     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 130 LPEMKADEVQSLLSAILTNQQTCLDGLKATA---GSWSLKNGLSLPLAADTKLYSMSLAL 189
           L   KAD++Q+LLSA LTN+QTCL+GL   A    +W+++ G++LPL  DTKL  +SLAL
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 190 FTKGWVPKKKKRPTWKAAGRQGG----------FRNGRMSLKMSSRTQAIYETATRRHLL 249
           FTKGWVPKKKKR  +  A  + G          FRNG + LKM+ +T+A+YE+ +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 250 QTDDGGD----DQISVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVY 309
             D  GD      + + DIV VSQDGTGNFT I  A+AAAPNN+  + G+FLI+V+AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 310 EEYVLIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNT 369
           EEY+ IAKNKRY+MMIGDGINQTVVTGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 370 AGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 429
           AG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 430 FQNCNIYPRLPMPNQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGR 489
           FQNCN+YPR PMPNQFNAITAQGR+DPNQNTGTSI NC I  ADDL ++    VKT+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN-YTVKTYLGR 485

Query: 490 PWKEYSRTVYLQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFH 549
           PWKEYSRTVY+QS++D  + P GWR W+GDFAL+T YYAE+ N GPGSNT+ RVTWPG+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 550 LINDSDAGNFTAANFVLADDWLPQTGVPYTSGL 560
           +IN +DA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of Lag0002605 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 686.4 bits (1770), Expect = 1.9e-197
Identity = 352/566 (62.19%), Postives = 438/566 (77.39%), Query Frame = 0

Query: 13  ILLIFLYLTT----STATPPPPPTGSVCSSTPDPSYCKSALPNQT-GNVYSYGRSSVRKS 72
           ++ +FL L T    S    P   + ++C +TPDP +CKS  P  + G+V  YGR S+RKS
Sbjct: 8   LVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKS 67

Query: 73  LSSSQKFLRLVEKHL-RSRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVN--TTSRTL 132
           L+ S+KF R ++++L R+ + L+ +AV AL+DC+ LA L  DYL TSF TVN  T+S+TL
Sbjct: 68  LTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 127

Query: 133 PEMKADEVQSLLSAILTNQQTCLDGLK-ATAGSWSLKNGLSLPLAADTKLYSMSLALFTK 192
              KADE+Q+LLSA LTN+QTCLDG+  A + SW+++NG++LPL  DTKL+S+SLALFTK
Sbjct: 128 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTK 187

Query: 193 GWVPKKKKRP---TWKAAGRQGG-------FRNGRMSLKMSSRTQAIYETATRRHLLQTD 252
           GWVPKKKK+    +W               FRNG + LKM+  T+A+YE+ +RR L   D
Sbjct: 188 GWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-D 247

Query: 253 DGGDDQISVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIA 312
           D   + + V DIV V+Q+GTGNFTTI EA+ +APN +  T GYF+I+V++GVYEE V+IA
Sbjct: 248 DNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIA 307

Query: 313 KNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQ 372
           KNKRYLMMIGDGIN+TVVTGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQ
Sbjct: 308 KNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQ 367

Query: 373 AVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIY 432
           AVA+R+ ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+Y
Sbjct: 368 AVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLY 427

Query: 433 PRLPMPNQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSR 492
           PR PM NQFNAITAQGRTDPNQNTG SIHNC I  ADDL ++    VKT+LGRPWKEYSR
Sbjct: 428 PRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSN-YTVKTYLGRPWKEYSR 487

Query: 493 TVYLQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDA 552
           TV++QS++D+++ P GWR W+GDFAL+T YYAE+ N G GS+T++RV WPG+H+IN +DA
Sbjct: 488 TVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDA 547

Query: 553 GNFTAANFVLADDWLPQTGVPYTSGL 560
            NFT  NF+L D W+ Q+GVPY SGL
Sbjct: 548 NNFTVENFLLGDGWMVQSGVPYISGL 571

BLAST of Lag0002605 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 661.0 bits (1704), Expect = 8.6e-190
Identity = 326/531 (61.39%), Postives = 412/531 (77.59%), Query Frame = 0

Query: 34  SVCSSTPDPSYCKSALPNQTGNVYSYGRSSVRKSLSSSQKFLRLVEKHLRSRSSLTV-AA 93
           ++C   PDPSYC+S LPNQ G++YSYGR S+R+SLS +++F+ +++  L  +  +   + 
Sbjct: 37  NICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKST 96

Query: 94  VRALEDCQVLAGLNMDYLTTSFRTVNTTSRTLPEMKADEVQSLLSAILTNQQTCLDGLKA 153
           V ALEDC+ LA L MDYL +S +T ++T +TL   +A++V + LSA +TN+QTCL+GLK+
Sbjct: 97  VGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTCLEGLKS 156

Query: 154 TAGSWSLKNGLSLPLAADTKLYSMSLALFTKGWVPKKKK-RPTWKAAGRQG---GFRNGR 213
           TA     +NGLS  L  DTKLY +SLALF+KGWVP++++ RP W+   R     GFRNG+
Sbjct: 157 TAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGK 216

Query: 214 MSLKMSSRTQAIYETATRRHLLQTDDGGDDQISVRDIVVVSQDGTGNFTTINEAIAAAPN 273
           + LKM+ R +A+Y T TRR LLQ+D    D + V DIV V Q+GTGNFTTIN AIAAAPN
Sbjct: 217 LPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAAIAAAPN 276

Query: 274 NSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSAT 333
            +  ++GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+TGNRSVVDGWTTFNSAT
Sbjct: 277 KTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSAT 336

Query: 334 FAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYR 393
           F + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHSLRQFYR
Sbjct: 337 FILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYR 396

Query: 394 DCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQFNAITAQGRTDPNQNTGTSIHNCRITA 453
           +CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+IH C I  
Sbjct: 397 ECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRP 456

Query: 454 ADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFMDDLINPAGWRAWDGDFALNTSYYAEFG 513
           ADDLA +    VKT+LGRPWKEYSRTV +Q+++D  + P+GW AW GDFAL+T YYAE+ 
Sbjct: 457 ADDLATSN-YTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYN 516

Query: 514 NFGPGSNTSERVTWPGFHLINDSDAGNFTAANFVLADDWLPQTGVPYTSGL 560
           N GPGS+T+ RVTWPG+H+IN +DA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 517 NTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of Lag0002605 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 485.7 bits (1249), Expect = 4.9e-137
Identity = 264/552 (47.83%), Postives = 352/552 (63.77%), Query Frame = 0

Query: 23  STATPPPPPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSVRKSLSSSQKFLRLVE 82
           S + P P P    C STP P  C+   SA+ +   + Y YG+ ++++ L  +++  +++ 
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 83  KHLR----SRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTTSRTLPEMKADEVQSL 142
           +  +       + TV  V A+ DC  LA L+++YL T    +   +  +     D V SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVTSL 194

Query: 143 LSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLALFTKGWVPKKKKRPTW 202
           L  ++TNQQTCLDGL      ++    +  PL   T+LYS+SL L +       K+    
Sbjct: 195 LGGVVTNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGS 254

Query: 203 KAAGRQGGFRNGRMSLKMSSRTQAIYETATR--------RHLLQTDDGGDDQISVRDIVV 262
           K  G+  G  N  +   + +  + + +T  +        R+L +  +     I VR+ V 
Sbjct: 255 K--GKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVT 314

Query: 263 VSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGIN 322
           V    T NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV+I+  KR +M+IGDGIN
Sbjct: 315 VGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGIN 374

Query: 323 QTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFY 382
           +T+++GN S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY
Sbjct: 375 KTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFY 434

Query: 383 LCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQFNAITA 442
            CSFE YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM NQ NA+TA
Sbjct: 435 RCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTA 494

Query: 443 QGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFMDDLINP 502
            GRTDPNQ TG SI NC I AA DLA +  + + TFLGRPWK YSRTVY+QS++ D++ P
Sbjct: 495 HGRTDPNQKTGISIINCTIGAAPDLAADPKSTM-TFLGRPWKPYSRTVYIQSYISDVVQP 554

Query: 503 AGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTAANFVLADDW 560
            GW  W+G   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D W
Sbjct: 555 VGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTW 614

BLAST of Lag0002605 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 483.0 bits (1242), Expect = 3.2e-136
Identity = 260/553 (47.02%), Postives = 354/553 (64.01%), Query Frame = 0

Query: 27  PPPPPTGS-----VCSSTPDPSYCK---SALPNQTGNVYSYGRSSVRKSLSSSQKFLRLV 86
           P  PP+ S      C STP P  C+   +A+ +   + Y YG+ ++++ L  + +  +++
Sbjct: 69  PSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 128

Query: 87  EKHLR----SRSSLTVAAVRALEDCQVLAGLNMDYLTTSFRTVNTTSRTLPEMKADEVQS 146
             + R       S T   + A+ DC  L+ L+++YL T   T   T++ +     + V S
Sbjct: 129 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLET-VTTELKTAQVMTAALVEHVNS 188

Query: 147 LLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLALFTKGWVPKKKKRPT 206
           LLS ++TNQQTCLDGL      ++    +  P+   T+LYS+SL L +       K+   
Sbjct: 189 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKA 248

Query: 207 WKAAGRQGGFRNGRMSLKMSSRTQAIYETA--------TRRHLLQTDDGGDDQISVRDIV 266
            K     GG  N      + +  + + +T         T R+L +  +     I V   V
Sbjct: 249 SKGKILGGG--NSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAV 308

Query: 267 VVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGI 326
           +V    + NFTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+++  NK+ LM++GDGI
Sbjct: 309 IVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGI 368

Query: 327 NQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTF 386
           N+T++TGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+F
Sbjct: 369 NKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSF 428

Query: 387 YLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMPNQFNAIT 446
           Y CSFE YQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM  Q NAIT
Sbjct: 429 YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAIT 488

Query: 447 AQGRTDPNQNTGTSIHNCRITAADDLANNTAAAVKTFLGRPWKEYSRTVYLQSFMDDLIN 506
           A GR DPNQNTG SI NC I AA DLA    +A+ TFLGRPWK YSRTV++QS++ D++ 
Sbjct: 489 AHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQ 548

Query: 507 PAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWPGFHLINDSDAGNFTAANFVLADD 560
           P GW  W+G   L+T YY E+ NFGPG+NT++RV W G++L+N ++A NFT  NF + D 
Sbjct: 549 PVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDT 608