Lag0002604 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002604 vs. NCBI nr
Match: XP_022991224.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 1011.5 bits (2614), Expect = 2.7e-291
Identity = 504/550 (91.64%), Postives = 522/550 (94.91%), Query Frame = 0

Query: 3   RDQIPSNP-ASISAIFTSFFFLFLFSSKPISSNPQIHQTALSLCNGTLYPHLCFSKLTSF 62
           R Q  SNP ASISAIFT F  LFLFS KPISSNPQ HQTA SLCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSI 122
           P+LASLSPEKLA SALNFT+ EVHGAYANC+KLKTL YG LNPTDRRALDDC+ELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSI 126

Query: 123 AELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+T I DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCS 362
           IK +RNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSD SAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDLIPVLS F+TYLGRPWKLYSRT+FL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

BLAST of Lag0002604 vs. NCBI nr
Match: XP_038896904.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.3e-290
Identity = 496/549 (90.35%), Postives = 519/549 (94.54%), Query Frame = 0

Query: 3   RDQIPSNPASISAIFTSFFFLFLFSSKPISSNPQIHQTALSLCNGTLYPHLCFSKLTSFP 62
           R QIPSNP SIS I T FF  F FS KPISSNPQIHQTA SLCNGTL+PHLCFSKL+SFP
Sbjct: 7   RAQIPSNPTSISTISTFFFIFFFFSFKPISSNPQIHQTARSLCNGTLFPHLCFSKLSSFP 66

Query: 63  ELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSIA 122
           +LASLSPEKLA SA+NFT  EV GAYANCTKLKT+LYG LNPTDRRALDDC+ELLD SIA
Sbjct: 67  QLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLELLDSSIA 126

Query: 123 ELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIEQ 182
           EL+  I DLAP QSAA HSHDLLTL+SAAMTNHRTC+DGFYNSSGTVRSRVELYLGKI Q
Sbjct: 127 ELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQ 186

Query: 183 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 242
           HLSIDLAMLKKIPGVNRA AA EVLPEYGAV+GGFP WVSVKDRRLLQAAVNETKF+LVV
Sbjct: 187 HLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNETKFNLVV 246

Query: 243 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 302
           AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIER+KTNLMFVGDGIGKTLI
Sbjct: 247 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDGIGKTLI 306

Query: 303 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSF 362
           K +RNVVDGWTTFRSATVA VG+GFIAKGITFEN+AGPSKHQAVALRSNSD SAFYQCSF
Sbjct: 307 KADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSF 366

Query: 363 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 422
           +GYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE
Sbjct: 367 VGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 426

Query: 423 DPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 482
           DPNQNTGISILNCKVEAASDLIPVLS F+TYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE
Sbjct: 427 DPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 486

Query: 483 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 542
           WNGTFALDTLYYGEY+NRGPGSNTTGRVTWPGYRVITN++EASQFTVE FIQG+SWLNST
Sbjct: 487 WNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGSSWLNST 546

Query: 543 GIPFFSGLA 552
           GIPFFSGLA
Sbjct: 547 GIPFFSGLA 555

BLAST of Lag0002604 vs. NCBI nr
Match: KAG6575805.1 (putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1003.0 bits (2592), Expect = 9.7e-289
Identity = 499/550 (90.73%), Postives = 520/550 (94.55%), Query Frame = 0

Query: 3   RDQIPSNP-ASISAIFTSFFFLFLFSSKPISSNPQIHQTALSLCNGTLYPHLCFSKLTSF 62
           R Q  SNP ASISAIFT F  +FL S KPISSNPQ HQTA SLCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSI 122
           P+LASLSPEKLA SALNFT+ EVHGAYANC+KLKTL YG LNPTDRRALDDC+ELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSI 126

Query: 123 AELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+T I DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCS 362
           IK +RNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSD SAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDL+PVL  F+TYLGRPWKLYSRTVFL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EW+GTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

BLAST of Lag0002604 vs. NCBI nr
Match: XP_023548978.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.8e-288
Identity = 499/550 (90.73%), Postives = 519/550 (94.36%), Query Frame = 0

Query: 3   RDQIPSNP-ASISAIFTSFFFLFLFSSKPISSNPQIHQTALSLCNGTLYPHLCFSKLTSF 62
           R Q  SNP ASISAIFT F  L L S KPISSNPQ HQTA SLCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSI 122
           P+LASLSPEKLA SALNFT+ EVHGAYANC+KLKTL YG L+PTDRRALDDC+ELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSI 126

Query: 123 AELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+T I DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCS 362
           IK NRNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSD SAFYQCS
Sbjct: 307 IKANRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGT+DFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDLIPVL  F+TYLGRPWKLYSRTVFL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLIPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

BLAST of Lag0002604 vs. NCBI nr
Match: KAG7014347.1 (putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 999.6 bits (2583), Expect = 1.1e-287
Identity = 499/550 (90.73%), Postives = 518/550 (94.18%), Query Frame = 0

Query: 3   RDQIPSNP-ASISAIFTSFFFLFLFSSKPISSNPQIHQTALSLCNGTLYPHLCFSKLTSF 62
           R Q  SNP ASISAIFT F  LFL S KPISSNPQ HQTA SLCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSI 122
           P+LASLSPEKLA SALNFT+ EVHGAYANC+KLKTL YG LNPTDRRALDDC+ELLD SI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDDSI 126

Query: 123 AELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+T I DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCS 362
           IK +RNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSD SAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDL+PVL  F+TYLGRPWKLYSRTVFL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

BLAST of Lag0002604 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.5e-154
Identity = 286/519 (55.11%), Postives = 364/519 (70.13%), Query Frame = 0

Query: 40  TALSLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLY 99
           T L L   T++      + TS  ++      +L  +ALN T + V+ + +N + L   L 
Sbjct: 13  TFLLLFLSTVFSSHVTQRFTSSDDIT-----ELVVTALNQTISNVNLSSSNFSDLLQRLG 72

Query: 100 GSLNPTDRRALDDCIELLDGSIAELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCI 159
            +L+  D  A DDC+ELLD ++ +L T IS L   +S +   H++  L+SAAMTN RTC+
Sbjct: 73  SNLSHRDLCAFDDCLELLDDTVFDLTTAISKL---RSHSPELHNVKMLLSAAMTNTRTCL 132

Query: 160 DGFYNSSGT------------VRSRVELYLGKIEQHLSIDLAMLKKIPGVNRATAAEEVL 219
           DGF +S               V   ++  L  I  H+S  LAML+ IPG       E+V 
Sbjct: 133 DGFASSDNDENLNNNDNKTYGVAESLKESLFNISSHVSDSLAMLENIPGHIPGKVKEDV- 192

Query: 220 PEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTR 279
                   GFPMWVS  DR LLQ  V+ETK +LVVA++G+GN+TT+GEA++AAPNSS TR
Sbjct: 193 --------GFPMWVSGSDRNLLQDPVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETR 252

Query: 280 FVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGF 339
           FVIYIK G YFEN+EI R+KT +MF+GDGIG+T+IK NR+  DGWT F SATV   GSGF
Sbjct: 253 FVIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGF 312

Query: 340 IAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTI 399
           IAK ++F N+AGP KHQAVALRS+SDLSA+Y+CSF  YQDT+YVHS +QFYRECD+YGT+
Sbjct: 313 IAKDLSFVNYAGPEKHQAVALRSSSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTV 372

Query: 400 DFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVL 459
           DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TAQGRE+  + TGISI++ ++ AA DLIPV 
Sbjct: 373 DFIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRENSREPTGISIISSRILAAPDLIPVQ 432

Query: 460 SLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTT 519
           + FK YLGRPW+LYSRTV ++SFI  LVDPAGWL+W   FAL+TLYYGEY+N GPGSN T
Sbjct: 433 ANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMT 492

Query: 520 GRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPF 547
            RV WPG++ I    EASQF+V  FI G  WLNST IPF
Sbjct: 493 NRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPF 514

BLAST of Lag0002604 vs. ExPASy Swiss-Prot
Match: P85076 (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 6.1e-145
Identity = 250/319 (78.37%), Postives = 274/319 (85.89%), Query Frame = 0

Query: 232 AVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLM 291
           AV +   D+VVAKDGSGNFTTVG AVAAA +SST RFVIYIK GAYFE V++++KKTNLM
Sbjct: 2   AVTDIVPDVVVAKDGSGNFTTVGAAVAAAKDSSTARFVIYIKEGAYFEYVDVDKKKTNLM 61

Query: 292 FVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSN 351
           F+GDGIGKT IKGNR+VVDGWTTFRS+TVA VG+GFIA+GI+FEN+AGPSKHQAVALRS 
Sbjct: 62  FIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSG 121

Query: 352 SDLSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 411
           +D SAFYQCSF+GYQDTLYVHSLRQFY ECDVYGTIDFIFGNAA V Q CNLYARKPN N
Sbjct: 122 ADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNEN 181

Query: 412 QRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFI 471
           Q+NIFTAQGR+DPNQNTGISILNCKV AA+DLIPVLS FKTYLGRPWK YSRTVFL S +
Sbjct: 182 QKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQM 241

Query: 472 GQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEA 531
             L+DPAGWLEW+G FAL TLYY EY N GPGSNTT RVTWPGY V TN TE  QFTV  
Sbjct: 242 ESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGN 301

Query: 532 FIQGTSWLNSTGIPFFSGL 551
           FIQG+ WL S  IP +  L
Sbjct: 302 FIQGSQWLTSYNIPVYLNL 320

BLAST of Lag0002604 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.0e-144
Identity = 268/487 (55.03%), Postives = 340/487 (69.82%), Query Frame = 0

Query: 71  KLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSIAELETLISD 130
           +L  + LN T  +V+ A +N + L+T L+ +L   +R A +DC+ LLD +I++LET +SD
Sbjct: 55  ELIIADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSD 114

Query: 131 LAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGK--------IEQ 190
           L   +S++   +D+  L++  MT   TC+DGF  S     + +   L +        I  
Sbjct: 115 L---RSSSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISN 174

Query: 191 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 250
           +LS  L ML+ I     +  + EV  EY       P W+S  D+RLL+A V ET ++L V
Sbjct: 175 NLSNSLHMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSV 234

Query: 251 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 310
           A DG+GNFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF+GDGIGKT+I
Sbjct: 235 AIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVI 294

Query: 311 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSF 370
           K NR+ +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F
Sbjct: 295 KANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEF 354

Query: 371 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 430
            GYQDTLYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R 
Sbjct: 355 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 414

Query: 431 DPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 490
             +Q TGISILNC++ AA DLIPV   FK YLGRPW+ YSRTV ++SFI  L+ PAGWLE
Sbjct: 415 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 474

Query: 491 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 550
               FAL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  FI G++WLNST
Sbjct: 475 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 531

BLAST of Lag0002604 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.6e-140
Identity = 270/529 (51.04%), Postives = 344/529 (65.03%), Query Frame = 0

Query: 32  SSNPQIHQTALSLCNGTLYPHLCFSKLTSFPELAS--LSPEKLASSALNFTDAEVHGAYA 91
           + N   H    S C+ T YP LCFS + + PE +    S + +   +LN T   V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 92  NCTKLKTLLYGSLNPTDRRALDDCIELLDGSIAELETLISDLAP---SQSAASHSHDLLT 151
              KL  L   +L   ++ AL DC+E +D ++ EL   + DL      +S + H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 152 LVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGK--IEQHLSIDLAMLKKIPGVN---RAT 211
           L+SAAMTN  TC+DGF +       R  L  G+  +E+  S  LAM+K +   +     T
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 212 AAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAP 271
           +    L E  +   G+P W+S  DRRLLQ+  +    + VVA DGSGNF TV  AVAAAP
Sbjct: 240 SNNRKLTEETSTVDGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAP 299

Query: 272 NSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVA 331
              T R++I IKAG Y ENVE+ +K  N+MF+GDG  +T+I G+RNVVDG TTF+SAT A
Sbjct: 300 QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAA 359

Query: 332 AVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSFIGYQDTLYVHSLRQFYREC 391
            VG GF+A+ ITF+N AGPSKHQAVALR  +DLSAFY C  + YQDTLYVHS RQF+  C
Sbjct: 360 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 419

Query: 392 DVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAAS 451
            + GT+DFIFGNAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A S
Sbjct: 420 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATS 479

Query: 452 DLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRG 511
           DL PV   F TYLGRPWK YSRTV ++S I  L+ PAGW EW+G FAL+TL+YGE+ N G
Sbjct: 480 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSG 539

Query: 512 PGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPFFSGL 551
            G+ T+GRV W G+RVIT++TEA  FT  +FI G+SWL STG PF  GL
Sbjct: 540 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Lag0002604 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.6e-140
Identity = 269/529 (50.85%), Postives = 346/529 (65.41%), Query Frame = 0

Query: 32  SSNPQIHQTALSLCNGTLYPHLCFSKLTSFPELAS--LSPEKLASSALNFTDAEVHGAYA 91
           + N   H    S C+ T YP LCFS + + PE +    S + +   +LN T   V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 92  NCTKLKTLLYGSLNPTDRRALDDCIELLDGSIAELETLISDLAP---SQSAASHSHDLLT 151
              KL  L   +L   ++ AL DC+E +D ++ EL   + DL      +S + H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 152 LVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGK--IEQHLSIDLAMLKKIPGVN---RAT 211
           L+SAAMTN  TC+DGF +       R  L  G+  +E+  S  LAM+K +   +     T
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 212 AAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAP 271
           +    L E  +   G+P W+S  DRRLLQ+  +    ++VVA DGSGNF TV  +VAAAP
Sbjct: 240 SNNRKLIEETSTVDGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAP 299

Query: 272 NSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVA 331
              T R++I IKAG Y ENVE+ +K  N+MF+GDG  +T+I G+RNVVDG TTF+SATVA
Sbjct: 300 QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 359

Query: 332 AVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSFIGYQDTLYVHSLRQFYREC 391
            VG GF+A+ ITF+N AGPSKHQAVALR  +DLSAFY C  + YQDTLYVHS RQF+  C
Sbjct: 360 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 419

Query: 392 DVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAAS 451
            + GT+DFIFGNAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A S
Sbjct: 420 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATS 479

Query: 452 DLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRG 511
           DL PV   F TYLGRPWK YSRTV ++S I  ++ PAGW EW+G FAL+TL+YGE+ N G
Sbjct: 480 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAG 539

Query: 512 PGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPFFSGL 551
            G+ T+GRV W G+RVIT++TEA  FT  +FI G+SWL STG PF  GL
Sbjct: 540 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Lag0002604 vs. ExPASy TrEMBL
Match: A0A6J1JU83 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.3e-291
Identity = 504/550 (91.64%), Postives = 522/550 (94.91%), Query Frame = 0

Query: 3   RDQIPSNP-ASISAIFTSFFFLFLFSSKPISSNPQIHQTALSLCNGTLYPHLCFSKLTSF 62
           R Q  SNP ASISAIFT F  LFLFS KPISSNPQ HQTA SLCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSI 122
           P+LASLSPEKLA SALNFT+ EVHGAYANC+KLKTL YG LNPTDRRALDDC+ELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSI 126

Query: 123 AELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+T I DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCS 362
           IK +RNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSD SAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDLIPVLS F+TYLGRPWKLYSRT+FL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

BLAST of Lag0002604 vs. ExPASy TrEMBL
Match: A0A6J1GNU4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 8.8e-288
Identity = 497/550 (90.36%), Postives = 518/550 (94.18%), Query Frame = 0

Query: 3   RDQIPSNP-ASISAIFTSFFFLFLFSSKPISSNPQIHQTALSLCNGTLYPHLCFSKLTSF 62
           R Q  SNP ASISAIFT F  LFL S KPISSNPQ HQTA SLCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSI 122
           P+LASLSPEKLA SALNFT+ EVHGAYANC+KLKTL YG LNPTDRRALDDC+ELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSI 126

Query: 123 AELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+T I DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQA+VNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVG AVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCS 362
           IK +RNVVDGWTTFRSATV AVG+GFIAKGITFENFAGPSKHQAVA RSNSD SAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAV+FQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDL+PVL  F+TYLGRPWKLYSRTVFL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

BLAST of Lag0002604 vs. ExPASy TrEMBL
Match: A0A1S3BSU3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 2.7e-284
Identity = 492/548 (89.78%), Postives = 511/548 (93.25%), Query Frame = 0

Query: 3   RDQIPSNPASISAIFTSFFFLFLFSSKPISSNPQIHQTALSLCNGTLYPHLCFSKLTSFP 62
           R QI SNP SIS I T FF  FLFSSKPISSNPQIHQTA SLCNGTL+P+LCFSKL+SFP
Sbjct: 7   RAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFSKLSSFP 66

Query: 63  ELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSIA 122
           +LASLSPEKLA SALNFT  EV  AYANCT LKT LY  LNPTDR ALDDC+ELLD SIA
Sbjct: 67  QLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLELLDDSIA 126

Query: 123 ELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIEQ 182
           EL+  I DL PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVRSRVELYLGKI Q
Sbjct: 127 ELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQ 186

Query: 183 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 242
           HLSIDLAMLKKIPGVNRAT  EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNETKF+LVV
Sbjct: 187 HLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVV 246

Query: 243 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 302
           AKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGDGIGKTLI
Sbjct: 247 AKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLI 306

Query: 303 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSF 362
           KG+RNVVDGWTTFRSATVA VG+GFIAKGITFEN+AGPSKHQAVALRSNSD SAFYQCSF
Sbjct: 307 KGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSF 366

Query: 363 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 422
           IGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE
Sbjct: 367 IGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 426

Query: 423 DPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 482
           DPNQNTGISILNCKVEAASDLIPVLS F+TYLGRPWKL+SRTVFLRSFIGQLV+P GWLE
Sbjct: 427 DPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLE 486

Query: 483 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 542
           WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITN+TEASQFTVE FIQG+SWLNST
Sbjct: 487 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGSSWLNST 546

Query: 543 GIPFFSGL 551
           GIPFFSGL
Sbjct: 547 GIPFFSGL 554

BLAST of Lag0002604 vs. ExPASy TrEMBL
Match: A0A5D3D1W6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001300 PE=3 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 3.5e-284
Identity = 491/548 (89.60%), Postives = 511/548 (93.25%), Query Frame = 0

Query: 3   RDQIPSNPASISAIFTSFFFLFLFSSKPISSNPQIHQTALSLCNGTLYPHLCFSKLTSFP 62
           R QI SNP SIS I T FF  FLFSSKPISSNPQIHQTA SLCNGTL+P+LCFSKL+SFP
Sbjct: 7   RAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFSKLSSFP 66

Query: 63  ELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSIA 122
           +LASLSPEKLA SALNFT  EV  AYANCT LKT LY  LNPTDR ALDDC+ELLD SIA
Sbjct: 67  QLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLELLDDSIA 126

Query: 123 ELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIEQ 182
           EL+  I DL PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVRSRVELYLGKI Q
Sbjct: 127 ELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQ 186

Query: 183 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 242
           HLSIDLAMLKKIPGVNRAT  EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNETKF+LVV
Sbjct: 187 HLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVV 246

Query: 243 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 302
           AKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGDGIGKTLI
Sbjct: 247 AKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLI 306

Query: 303 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSF 362
           KG+RNVVDGWTTFRSATVA +G+GFIAKGITFEN+AGPSKHQAVALRSNSD SAFYQCSF
Sbjct: 307 KGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSF 366

Query: 363 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 422
           IGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE
Sbjct: 367 IGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 426

Query: 423 DPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 482
           DPNQNTGISILNCKVEAASDLIPVLS F+TYLGRPWKL+SRTVFLRSFIGQLV+P GWLE
Sbjct: 427 DPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLE 486

Query: 483 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 542
           WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITN+TEASQFTVE FIQG+SWLNST
Sbjct: 487 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGSSWLNST 546

Query: 543 GIPFFSGL 551
           GIPFFSGL
Sbjct: 547 GIPFFSGL 554

BLAST of Lag0002604 vs. ExPASy TrEMBL
Match: A0A0A0K5K8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.8e-265
Identity = 459/522 (87.93%), Postives = 482/522 (92.34%), Query Frame = 0

Query: 30  PISSNPQIHQTALSLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEVHGAYA 89
           P+      HQTA SLCNGTL+P LCFSKL+SFP+LASLSPEKLA SALNFT  EV  AY 
Sbjct: 90  PLPLFQTFHQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYT 149

Query: 90  NCTKLKTLLYGSLNPTDRRALDDCIELLDGSIAELETLISDLAPSQSAASHSHDLLTLVS 149
           NCT LKT LY  LNPTDR ALDDC+ELLDGSIAEL+  I DLAPSQS A HSHDLLTLVS
Sbjct: 150 NCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVS 209

Query: 150 AAMTNHRTCIDGFYNSSGTVRSRVELYLGKIEQHLSIDLAMLKKIPGVNRATAA-EEVLP 209
           AAMTNHRTC+DGFYNSSGTVRSRVELYLGKI QHLSIDLAMLKKIPGVNRAT   +E+LP
Sbjct: 210 AAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLP 269

Query: 210 EYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTRF 269
           EYGAV+GGFP WVSVKDRRLLQAAVNETKF++VVAKDGSGNFTTV EAVAAAPN+STTRF
Sbjct: 270 EYGAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRF 329

Query: 270 VIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGFI 329
           VIYIKAGAYFENVEI R K+NLMFVGDGIGKTLIK +RNVVDGWTTFRSATVA VG+GFI
Sbjct: 330 VIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFI 389

Query: 330 AKGITFENFAGPSKHQAVALRSNSDLSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTID 389
           AKGITFEN+AGPSKHQAVALRSNSD SAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTID
Sbjct: 390 AKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTID 449

Query: 390 FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS 449
           FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS
Sbjct: 450 FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS 509

Query: 450 LFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTG 509
            F+TYLGRPWKLYSRTVFLRSFIGQL++P GWLEWNGTFALDTLYYGEYLNRGPGSNTT 
Sbjct: 510 SFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTM 569

Query: 510 RVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPFFSGL 551
           RVTWPGYRVITN+TEASQFTVE FIQG+SWLNST IPFFSGL
Sbjct: 570 RVTWPGYRVITNATEASQFTVERFIQGSSWLNSTEIPFFSGL 611

BLAST of Lag0002604 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 547.7 bits (1410), Expect = 1.0e-155
Identity = 286/519 (55.11%), Postives = 364/519 (70.13%), Query Frame = 0

Query: 40  TALSLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLY 99
           T L L   T++      + TS  ++      +L  +ALN T + V+ + +N + L   L 
Sbjct: 13  TFLLLFLSTVFSSHVTQRFTSSDDIT-----ELVVTALNQTISNVNLSSSNFSDLLQRLG 72

Query: 100 GSLNPTDRRALDDCIELLDGSIAELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCI 159
            +L+  D  A DDC+ELLD ++ +L T IS L   +S +   H++  L+SAAMTN RTC+
Sbjct: 73  SNLSHRDLCAFDDCLELLDDTVFDLTTAISKL---RSHSPELHNVKMLLSAAMTNTRTCL 132

Query: 160 DGFYNSSGT------------VRSRVELYLGKIEQHLSIDLAMLKKIPGVNRATAAEEVL 219
           DGF +S               V   ++  L  I  H+S  LAML+ IPG       E+V 
Sbjct: 133 DGFASSDNDENLNNNDNKTYGVAESLKESLFNISSHVSDSLAMLENIPGHIPGKVKEDV- 192

Query: 220 PEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTR 279
                   GFPMWVS  DR LLQ  V+ETK +LVVA++G+GN+TT+GEA++AAPNSS TR
Sbjct: 193 --------GFPMWVSGSDRNLLQDPVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETR 252

Query: 280 FVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGF 339
           FVIYIK G YFEN+EI R+KT +MF+GDGIG+T+IK NR+  DGWT F SATV   GSGF
Sbjct: 253 FVIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGF 312

Query: 340 IAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTI 399
           IAK ++F N+AGP KHQAVALRS+SDLSA+Y+CSF  YQDT+YVHS +QFYRECD+YGT+
Sbjct: 313 IAKDLSFVNYAGPEKHQAVALRSSSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTV 372

Query: 400 DFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVL 459
           DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TAQGRE+  + TGISI++ ++ AA DLIPV 
Sbjct: 373 DFIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRENSREPTGISIISSRILAAPDLIPVQ 432

Query: 460 SLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTT 519
           + FK YLGRPW+LYSRTV ++SFI  LVDPAGWL+W   FAL+TLYYGEY+N GPGSN T
Sbjct: 433 ANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMT 492

Query: 520 GRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPF 547
            RV WPG++ I    EASQF+V  FI G  WLNST IPF
Sbjct: 493 NRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPF 514

BLAST of Lag0002604 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 515.0 bits (1325), Expect = 7.5e-146
Identity = 268/487 (55.03%), Postives = 340/487 (69.82%), Query Frame = 0

Query: 71  KLASSALNFTDAEVHGAYANCTKLKTLLYGSLNPTDRRALDDCIELLDGSIAELETLISD 130
           +L  + LN T  +V+ A +N + L+T L+ +L   +R A +DC+ LLD +I++LET +SD
Sbjct: 55  ELIIADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSD 114

Query: 131 LAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGK--------IEQ 190
           L   +S++   +D+  L++  MT   TC+DGF  S     + +   L +        I  
Sbjct: 115 L---RSSSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISN 174

Query: 191 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 250
           +LS  L ML+ I     +  + EV  EY       P W+S  D+RLL+A V ET ++L V
Sbjct: 175 NLSNSLHMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSV 234

Query: 251 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 310
           A DG+GNFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF+GDGIGKT+I
Sbjct: 235 AIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVI 294

Query: 311 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSF 370
           K NR+ +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F
Sbjct: 295 KANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEF 354

Query: 371 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 430
            GYQDTLYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R 
Sbjct: 355 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 414

Query: 431 DPNQNTGISILNCKVEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 490
             +Q TGISILNC++ AA DLIPV   FK YLGRPW+ YSRTV ++SFI  L+ PAGWLE
Sbjct: 415 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 474

Query: 491 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 550
               FAL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  FI G++WLNST
Sbjct: 475 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 531

BLAST of Lag0002604 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 500.0 bits (1286), Expect = 2.5e-141
Identity = 272/530 (51.32%), Postives = 337/530 (63.58%), Query Frame = 0

Query: 38  HQTALSLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEVHGAYANCTKLKTL 97
           H    S+C+ TLYP LCFS + +       S +++  ++LN T   V   Y    KL   
Sbjct: 59  HAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAK 118

Query: 98  LYGSLNPTDRRALDDCIELLDGSIAELETLISDL---APSQSAASHSHDLLTLVSAAMTN 157
             G L P +  AL DC+E +D ++ EL   + DL      +S   H+ DL TL+S+A+TN
Sbjct: 119 RKG-LTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 178

Query: 158 HRTCIDGFYNSSGTVRSRVELYLGK--IEQHLSIDLAMLKKIPGV--------------- 217
             TC+DGF       + R  L  G+  +E   S  LAM+K +                  
Sbjct: 179 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFT 238

Query: 218 -NRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEA 277
            N     +EV  +  +   G+P W+SV DRRLLQ +    K D  VA DGSG+FTTV  A
Sbjct: 239 NNNNRKLKEVTGDLDS--DGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAA 298

Query: 278 VAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFR 337
           VAAAP  S  RFVI+IKAG Y ENVE+ +KKTN+MF+GDG GKT+I G+RNVVDG TTF 
Sbjct: 299 VAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFH 358

Query: 338 SATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSFIGYQDTLYVHSLRQ 397
           SATVAAVG  F+A+ ITF+N AGPSKHQAVALR  SD SAFYQC    YQDTLYVHS RQ
Sbjct: 359 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQ 418

Query: 398 FYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCK 457
           F+ +C + GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC+
Sbjct: 419 FFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCR 478

Query: 458 VEAASDLIPVLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGE 517
           +   SDL+ V   F TYLGRPWK YSRTV ++S I  ++ P GW EW+G+FALDTL Y E
Sbjct: 479 IGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYRE 538

Query: 518 YLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPF 547
           YLNRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL STG PF
Sbjct: 539 YLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of Lag0002604 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 484.2 bits (1245), Expect = 1.4e-136
Identity = 256/519 (49.33%), Postives = 335/519 (64.55%), Query Frame = 0

Query: 45  CNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEVHGAYANCTKLKTLLYGSLNP 104
           C+ +LYP+LC   L  FP   +    +L   + N T  +   A    +   T+ Y  + P
Sbjct: 79  CSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS---TITYTQMPP 138

Query: 105 TDRRALDDCIELLDGSIAELETLISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGF-- 164
             R A D C+ELLD S+  L   +S +       SHS D++T +S+AMTNH TC DGF  
Sbjct: 139 RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-DVMTWLSSAMTNHDTCTDGFDE 198

Query: 165 -YNSSGTVRSRVELYLGKIEQHLSIDLAM----LKKIPGV----NRATAAEEVLPEYGAV 224
                G V+ +V   +  + + +S  LA+    +K + GV    NR     E   E    
Sbjct: 199 IEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE---- 258

Query: 225 RGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIK 284
               P W+  +DR LL    +  + D+ V+KDGSG F T+ EA+  AP  S+ RFVIY+K
Sbjct: 259 ---LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVK 318

Query: 285 AGAY-FENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGFIAKGI 344
           AG Y  EN+++ RKKTNLMF+GDG GKT+I G +++ D  TTF +AT AA G+GFI + +
Sbjct: 319 AGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDM 378

Query: 345 TFENFAGPSKHQAVALRSNSDLSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFG 404
           TFEN+AGP+KHQAVALR   D +  Y+C+ IGYQD LYVHS RQF+REC++YGT+DFIFG
Sbjct: 379 TFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFG 438

Query: 405 NAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSLFKT 464
           NAAV+ Q+CN+YARKP + Q+   TAQ R+DPNQNTGISI  CK+ A  DL      + T
Sbjct: 439 NAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPT 498

Query: 465 YLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTW 524
           YLGRPWKLYSR V++ S +G  +DP GWLEWNG FALD+LYYGEY+N+G GS    RV W
Sbjct: 499 YLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKW 558

Query: 525 PGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPFFSGLA 552
           PGY VIT++ EAS+FTV  FI G+SWL STG+ FFSGL+
Sbjct: 559 PGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of Lag0002604 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 479.9 bits (1234), Expect = 2.7e-135
Identity = 258/526 (49.05%), Postives = 340/526 (64.64%), Query Frame = 0

Query: 39  QTALSLCNGTLYPHLCFSKLTSFP-ELASLSPEKLASSALNFTDAEV-HGAYANCTKLKT 98
           Q     C  T +P LC   L  FP  LA+ S + L    +N T     H  Y++     +
Sbjct: 85  QAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSA----S 144

Query: 99  LLYGSLNPTDRRALDDCIELLDGSIAELETLISDLAPSQSAASHSHDLLTLVSAAMTNHR 158
           L +  + P  R A D C+ELLD S+  L   +S +    S+++   D+ T +SAA+TNH 
Sbjct: 145 LSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVV---SSSAKPQDVTTWLSAALTNHD 204

Query: 159 TCIDGFYN-SSGTVRSRVELYLGKIEQHLSIDLAMLKK---------IPGVNRATAAEEV 218
           TC +GF     G V+  +   L  + + +S  LA+            +P  NR     E 
Sbjct: 205 TCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEE 264

Query: 219 LPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTT 278
             E       FP W+  K+R +L+  V++ + D++V+KDG+G   T+ EA+  AP +ST 
Sbjct: 265 REE------KFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTR 324

Query: 279 RFVIYIKAGAYFE-NVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGS 338
           R +IY+KAG Y E N+++ RKK NLMFVGDG GKT+I G +++ D  TTF +A+ AA G+
Sbjct: 325 RIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGA 384

Query: 339 GFIAKGITFENFAGPSKHQAVALRSNSDLSAFYQCSFIGYQDTLYVHSLRQFYRECDVYG 398
           GFIA+ ITFEN+AGP+KHQAVALR  +D +  Y+C+ IGYQDTLYVHS RQF+RECD+YG
Sbjct: 385 GFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYG 444

Query: 399 TIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIP 458
           T+DFIFGNAAVV QNC++YARKP   Q+N  TAQ R+DPNQNTGISI   +V AASDL  
Sbjct: 445 TVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQA 504

Query: 459 VLSLFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSN 518
                +TYLGRPWKL+SRTV++ S+IG  V   GWLEWN TFALDTLYYGEYLN GPGS 
Sbjct: 505 TNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSG 564

Query: 519 TTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPFFSGLA 552
              RV+WPGYRVI ++ EA++FTV  FI G+SWL STG+ F +GL+
Sbjct: 565 LGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597