Lag0002572 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002572
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Exopolygalacturonase-like
Location	chr4: 43906960 .. 43907674 (-)
RNA-Seq Expression	Lag0002572
Synteny	Lag0002572

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTCCGATTCGACGTTCATGTGTGATCCAGTGGGTTTCGATTCTCCGTTCGTTCTCGCCATTTGGAACCTCCTTTCGGCGAGGTCGATTCGAGAGCGATGTTCGGGTTCAGGGCAATCGAAAGGCGCGAGGAAGAAAACGCCGAAGGGAACCCACACATTTGATTTCGATTTTACGAGTTTCTCAAAAAGTTGATAGGAACCCACACATGAAACCCACATCGGCCTCATATCTAAACCCACGACGACCTCATATCTAGCGTCTCCTTGCTGGAATGCGTGTGTTCCTTTGTTGGCCTCAGATCTGAAGTCATGGGCGTTCTCTCAAACTTATGGTACAAAGAAGAACAAGGTAACCAACCACAGAAAAACAAACTAATCTACGTTCTTTTCAGAACATTTATCATACACCATCAACTTAATTAAGCTTTTGAGTTTAGTAGTAACTTTACGCTCTACAATTTGATCATGTGTAGGCTTCGAAGTGGAAGATCAGCGACGTTCACTTCAAGAACATCCGTGGAACGTCGGTGACAAACGTGGCGGTGTTGTTGGAGTGCAGCGCGTTGTTTCCATGTAAGGGGGTGGAGCTGAGGGACATTAACTTGACCTATGGGGGCGACAACTTGAGGAATACGACCATTGTTTCTTCATGTTTGAATGCACAAATTGAACATCATGGGGTGCAGAACTCACCGCCTTGTGTTGTATAA

mRNA sequence

ATGAGTTCCGATTCGACGTTCATGTGTGATCCAGTGGGTTTCGATTCTCCGTTCGTTCTCGCCATTTGGAACCTCCTTTCGGCGAGGTCGATTCGAGAGCGATGTTCGGGTTCAGGGCAATCGAAAGGCGCGAGGAAGAAAACGCCGAAGGGAACCCACACATTTGATTTCGATTTTACGAGTTTCTCAAAAAATCTGAAGTCATGGGCGTTCTCTCAAACTTATGGTACAAAGAAGAACAAGGCTTCGAAGTGGAAGATCAGCGACGTTCACTTCAAGAACATCCGTGGAACGTCGGTGACAAACGTGGCGGTGTTGTTGGAGTGCAGCGCGTTGTTTCCATGTAAGGGGGTGGAGCTGAGGGACATTAACTTGACCTATGGGGGCGACAACTTGAGGAATACGACCATTGTTTCTTCATGTTTGAATGCACAAATTGAACATCATGGGGTGCAGAACTCACCGCCTTGTGTTGTATAA

Coding sequence (CDS)

ATGAGTTCCGATTCGACGTTCATGTGTGATCCAGTGGGTTTCGATTCTCCGTTCGTTCTCGCCATTTGGAACCTCCTTTCGGCGAGGTCGATTCGAGAGCGATGTTCGGGTTCAGGGCAATCGAAAGGCGCGAGGAAGAAAACGCCGAAGGGAACCCACACATTTGATTTCGATTTTACGAGTTTCTCAAAAAATCTGAAGTCATGGGCGTTCTCTCAAACTTATGGTACAAAGAAGAACAAGGCTTCGAAGTGGAAGATCAGCGACGTTCACTTCAAGAACATCCGTGGAACGTCGGTGACAAACGTGGCGGTGTTGTTGGAGTGCAGCGCGTTGTTTCCATGTAAGGGGGTGGAGCTGAGGGACATTAACTTGACCTATGGGGGCGACAACTTGAGGAATACGACCATTGTTTCTTCATGTTTGAATGCACAAATTGAACATCATGGGGTGCAGAACTCACCGCCTTGTGTTGTATAA

Protein sequence

MSSDSTFMCDPVGFDSPFVLAIWNLLSARSIRERCSGSGQSKGARKKTPKGTHTFDFDFTSFSKNLKSWAFSQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNLRNTTIVSSCLNAQIEHHGVQNSPPCVV

Homology

[Close]

BLAST of Lag0002572 vs. NCBI nr
Match: XP_023548983.1 (exopolygalacturonase clone GBGE184-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 153.3 bits (386), Expect = 1.8e-33
Identity = 82/132 (62.12%), Postives = 90/132 (68.18%), Query Frame = 0


Query: 33  ERCSGSGQSKGARKKTPKGTHT-----FDFDFTSFSKNLKSWAFSQTYGTKKNKASKWKI 92
           + C+    + G R KT  G  T       FD     K        QTYGTKKNKASKWKI
Sbjct: 253 QNCTLFNTTNGVRIKTWAGMVTGSATRITFDNIVMDKVKNPIIIDQTYGTKKNKASKWKI 312

Query: 93  SDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNLRNTTIVSSCLNAQIE 152
           SD+HFKNIRGTS TNVAVLLECS LFPC+GVELRDINL+YGG +LRNTT VSSCLNA+I 
Sbjct: 313 SDIHFKNIRGTSTTNVAVLLECSELFPCEGVELRDINLSYGGASLRNTTTVSSCLNAKIT 372

Query: 153 HHGVQNSPPCVV 160
             GVQN P C V
Sbjct: 373 TSGVQNPPACAV 384

[Close]

BLAST of Lag0002572 vs. NCBI nr
Match: XP_022991230.1 (exopolygalacturonase clone GBGE184-like [Cucurbita maxima])

HSP 1 Score: 153.3 bits (386), Expect = 1.8e-33
Identity = 81/131 (61.83%), Postives = 92/131 (70.23%), Query Frame = 0


Query: 33  ERCSGSGQSKGARKKTPKGTHT-----FDFDFTSFSKNLKSWAFSQTYGTKKNKASKWKI 92
           + C+    + G R KT  GT +       FD     K        QTYGTKKNKASKWKI
Sbjct: 253 QNCTLFNTTNGVRIKTWAGTVSGSAVGITFDNIVMDKVKNPIIIDQTYGTKKNKASKWKI 312

Query: 93  SDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNLRNTTIVSSCLNAQIE 152
           SDVHFKNIRGTS TNVAV+LECS LFPC+GVELRDINL+YGG +L+NTT VSSCLNA+I+
Sbjct: 313 SDVHFKNIRGTSTTNVAVMLECSELFPCEGVELRDINLSYGGASLKNTTTVSSCLNAKIK 372

Query: 153 HHGVQNSPPCV 159
             GVQN P CV
Sbjct: 373 TFGVQNPPACV 383

[Close]

BLAST of Lag0002572 vs. NCBI nr
Match: XP_022953711.1 (exopolygalacturonase clone GBGE184-like [Cucurbita moschata])

HSP 1 Score: 152.1 bits (383), Expect = 3.9e-33
Identity = 82/131 (62.60%), Postives = 90/131 (68.70%), Query Frame = 0


Query: 33  ERCSGSGQSKGARKKTPKGTHT-----FDFDFTSFSKNLKSWAFSQTYGTKKNKASKWKI 92
           + C+    + G R KT  G  T       FD     K        QTYGTKKNKASKWKI
Sbjct: 253 QNCTLFNTTNGVRIKTWAGLVTGSAMRITFDNIVMDKVKNPIIIDQTYGTKKNKASKWKI 312

Query: 93  SDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNLRNTTIVSSCLNAQIE 152
           SDVHFKNIRGTS TNVAVLLECS LFPC+GVELRDINL+YGG +LRNTT VSSCLNA+I+
Sbjct: 313 SDVHFKNIRGTSTTNVAVLLECSELFPCEGVELRDINLSYGGASLRNTTTVSSCLNAKIK 372

Query: 153 HHGVQNSPPCV 159
             G QN P CV
Sbjct: 373 TFGEQNPPACV 383

[Close]

BLAST of Lag0002572 vs. NCBI nr
Match: KAG6575808.1 (Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 152.1 bits (383), Expect = 3.9e-33
Identity = 82/131 (62.60%), Postives = 90/131 (68.70%), Query Frame = 0


Query: 33  ERCSGSGQSKGARKKTPKGTHT-----FDFDFTSFSKNLKSWAFSQTYGTKKNKASKWKI 92
           + C+    + G R KT  G  T       FD     K        QTYGTKKNKASKWKI
Sbjct: 271 QNCTLFNTTNGVRIKTWAGMVTGSAMRITFDNIVMDKVKNPIIIDQTYGTKKNKASKWKI 330

Query: 93  SDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNLRNTTIVSSCLNAQIE 152
           SDVHFKNIRGTS TNVAVLLECS LFPC+GVELRDINL+YGG +LRNTT VSSCLNA+I+
Sbjct: 331 SDVHFKNIRGTSTTNVAVLLECSELFPCEGVELRDINLSYGGASLRNTTTVSSCLNAKIK 390

Query: 153 HHGVQNSPPCV 159
             G QN P CV
Sbjct: 391 TFGEQNPPACV 401

[Close]

BLAST of Lag0002572 vs. NCBI nr
Match: KAA0048661.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >KAA0048665.1 exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK18163.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 152.1 bits (383), Expect = 3.9e-33
Identity = 73/87 (83.91%), Postives = 75/87 (86.21%), Query Frame = 0


Query: 73  QTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNL 132
           QTYGTKK K S WKISDVHFKNIRGTS TNVAVLLECS L PC+GVELRDINLTYGG NL
Sbjct: 371 QTYGTKKKKVSNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINLTYGGTNL 430

Query: 133 RNTTIVSSCLNAQIEHHGVQNSPPCVV 160
           RNTTIVSSC NA+I   GVQN PPCVV
Sbjct: 431 RNTTIVSSCSNAKIATFGVQNPPPCVV 457

[Close]

BLAST of Lag0002572 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.8e-09
Identity = 46/132 (34.85%), Postives = 61/132 (46.21%), Query Frame = 0


Query: 35  CSGSGQSKGARKKTPKGTH---TFDFDFTSFSKN------LKSWAFSQTYGTKKNKASKW 94
           C+ +  S GAR KT  G H     +  F   + N      L    +      KKN+ SK 
Sbjct: 268 CTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKV 327

Query: 95  KISDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNLRNTTIVSSCLNAQ 154
           K+S++ FKNIRGTS    A+   CS   PC+ VEL DI++ + G         S CLN +
Sbjct: 328 KLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHNGAE----PATSQCLNVK 387

Query: 155 IEHHGVQNSPPC 158
               G  N  PC
Sbjct: 388 PITSGKLNPIPC 395

[Close]

BLAST of Lag0002572 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.1e-09
Identity = 46/132 (34.85%), Postives = 61/132 (46.21%), Query Frame = 0


Query: 35  CSGSGQSKGARKKTPKGTH---TFDFDFTSFSKN------LKSWAFSQTYGTKKNKASKW 94
           C+ +  S GAR KT  G H     +  F   + N      L    +      KKN+ SK 
Sbjct: 268 CTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKV 327

Query: 95  KISDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNLRNTTIVSSCLNAQ 154
           K+S++ FKNIRGTS    A+   CS   PC+ VEL DI++ + G         S CLN +
Sbjct: 328 KLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGAE----PATSQCLNVK 387

Query: 155 IEHHGVQNSPPC 158
               G  N  PC
Sbjct: 388 PITIGKLNPIPC 395

[Close]

BLAST of Lag0002572 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.2e-08
Identity = 29/47 (61.70%), Postives = 33/47 (70.21%), Query Frame = 0


Query: 83  SKWKISDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGG 130
           S  K+S + FKNI+GTS T  AV L CS  FPC GVEL DI+LTY G
Sbjct: 283 SSVKLSKISFKNIKGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSG 329

[Close]

BLAST of Lag0002572 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 3.5e-08
Identity = 34/78 (43.59%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 80  NKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNLRNTTIVS 139
           N ASK  + DV FKNI GTS T  AV L C+A  PC GV + D+N+ Y G N +   I  
Sbjct: 335 NGASKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAI-- 394

Query: 140 SCLNAQIEHHGVQNSPPC 158
            C NA+    G      C
Sbjct: 395 -CTNAKGSTKGCLKELAC 409

[Close]

BLAST of Lag0002572 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 4.5e-08
Identity = 33/78 (42.31%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 80  NKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCKGVELRDINLTYGGDNLRNTTIVS 139
           N ASK  + DV FKNI GTS T  AV L C+A  PC GV + D+N+ Y G N +   +  
Sbjct: 335 NGASKVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAV-- 394

Query: 140 SCLNAQIEHHGVQNSPPC 158
            C NA+    G      C
Sbjct: 395 -CKNAKGSAKGCLKELAC 409

The following BLAST results are available for this feature:

BLAST of Lag0002572 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548983.1	1.8e-33	62.12	exopolygalacturonase clone GBGE184-like [Cucurbita pepo subsp. pepo]	[more]
XP_022991230.1	1.8e-33	61.83	exopolygalacturonase clone GBGE184-like [Cucurbita maxima]	[more]
XP_022953711.1	3.9e-33	62.60	exopolygalacturonase clone GBGE184-like [Cucurbita moschata]	[more]
KAG6575808.1	3.9e-33	62.60	Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAA0048661.1	3.9e-33	83.91	exopolygalacturonase-like [Cucumis melo var. makuwa] >KAA0048665.1 exopolygalact...	[more]

BLAST of Lag0002572 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39786	1.8e-09	34.85	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]
Q39766	3.1e-09	34.85	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
P24548	1.2e-08	61.70	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
P26216	3.5e-08	43.59	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1	[more]
P35339	4.5e-08	42.31	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 76..134 e-value: 1.8E-10 score: 40.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 29..157 e-value: 1.3E-14 score: 55.9
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 71..155
None	No IPR available	PANTHER	PTHR31375:SF158	POLYGALACTURONASE	coord: 71..155
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 77..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002572.1	Lag0002572.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity