Lag0002557 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002557
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	pectinesterase inhibitor 6
Location	chr4: 43813161 .. 43813754 (+)
RNA-Seq Expression	Lag0002557
Synteny	Lag0002557

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCGTGCACCCAGTTCGAGACTGGCAATCGCTTCCATTTTAGCCCTCGTCTCTGCTCTTCCATGGCTGACCCACTCAGCCAAAACGAGCTATGTCCGAGAAGCCTGCAGCGTGACGCTGCACCCAGATCTCTGCGTCCAATCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCCACGAAGTGGGCAAGGGCGGGCGTGTCGGTGACGATAACAGAGGCCAAAACCGTGGCGCGATTCTTGGGGAGGCTGAAGAACAGCGGGCGTATGAAGGGGAAAAACAGGGCCGCAGTTTTGGACTGTGTGGAGGTCTTCGAAGCCGCCATTGATGAGCTTCATAGGTCGCTTGGGGTTCTTAGAAGATTGAGTAGGAGCAATTTTGACACTCAAATGGGCGATCTGACGACGTGGGTCAGCGCTGCGCTGACCGATGAAGATACTTGCGTCGATGGATTCGAAGGGGAAAGGGGAAAAGTGGTGAATTTGATACGGAATCGCGTGTTGAAGGCTAGTTATATTACGAGCAATGCACTTGCTTTGGTGAACAAACTGGCTGCCTCTGGTTTTGAAACTGCCCCTAATCAGTGA

mRNA sequence

ATGTTGCGTGCACCCAGTTCGAGACTGGCAATCGCTTCCATTTTAGCCCTCGTCTCTGCTCTTCCATGGCTGACCCACTCAGCCAAAACGAGCTATGTCCGAGAAGCCTGCAGCGTGACGCTGCACCCAGATCTCTGCGTCCAATCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCCACGAAGTGGGCAAGGGCGGGCGTGTCGGTGACGATAACAGAGGCCAAAACCGTGGCGCGATTCTTGGGGAGGCTGAAGAACAGCGGGCGTATGAAGGGGAAAAACAGGGCCGCAGTTTTGGACTGTGTGGAGGTCTTCGAAGCCGCCATTGATGAGCTTCATAGGTCGCTTGGGGTTCTTAGAAGATTGAGTAGGAGCAATTTTGACACTCAAATGGGCGATCTGACGACGTGGGTCAGCGCTGCGCTGACCGATGAAGATACTTGCGTCGATGGATTCGAAGGGGAAAGGGGAAAAGTGGTGAATTTGATACGGAATCGCGTGTTGAAGGCTAGTTATATTACGAGCAATGCACTTGCTTTGGTGAACAAACTGGCTGCCTCTGGTTTTGAAACTGCCCCTAATCAGTGA

Coding sequence (CDS)

ATGTTGCGTGCACCCAGTTCGAGACTGGCAATCGCTTCCATTTTAGCCCTCGTCTCTGCTCTTCCATGGCTGACCCACTCAGCCAAAACGAGCTATGTCCGAGAAGCCTGCAGCGTGACGCTGCACCCAGATCTCTGCGTCCAATCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCCACGAAGTGGGCAAGGGCGGGCGTGTCGGTGACGATAACAGAGGCCAAAACCGTGGCGCGATTCTTGGGGAGGCTGAAGAACAGCGGGCGTATGAAGGGGAAAAACAGGGCCGCAGTTTTGGACTGTGTGGAGGTCTTCGAAGCCGCCATTGATGAGCTTCATAGGTCGCTTGGGGTTCTTAGAAGATTGAGTAGGAGCAATTTTGACACTCAAATGGGCGATCTGACGACGTGGGTCAGCGCTGCGCTGACCGATGAAGATACTTGCGTCGATGGATTCGAAGGGGAAAGGGGAAAAGTGGTGAATTTGATACGGAATCGCGTGTTGAAGGCTAGTTATATTACGAGCAATGCACTTGCTTTGGTGAACAAACTGGCTGCCTCTGGTTTTGAAACTGCCCCTAATCAGTGA

Protein sequence

MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRSPTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNALALVNKLAASGFETAPNQ

Homology

[Close]

BLAST of Lag0002557 vs. NCBI nr
Match: KAG6593199.1 (Pectinesterase inhibitor 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 335.9 bits (860), Expect = 2.4e-88
Identity = 170/197 (86.29%), Postives = 185/197 (93.91%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           MLRAP+SR +I ++LA +S LPWLTHSA+TSYV+EACSVTLHPDLC++SLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARA VSVTITEAK VARFL RLKN+ RMKG+NRAAV DCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSRS FD QMGDLTTWVSAALTDEDTCVDGFEGERG++VNLIRNRV+KA+YITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPNQ 198
           ALVNKLAASGFETAPNQ
Sbjct: 181 ALVNKLAASGFETAPNQ 197

[Close]

BLAST of Lag0002557 vs. NCBI nr
Match: XP_023514751.1 (pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo] >KAG7030218.1 Pectinesterase inhibitor 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 334.0 bits (855), Expect = 9.0e-88
Identity = 169/196 (86.22%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           MLRAP+SR +I ++LA +S LPWLTHSA+TSYV+EACSVTLHPDLC++SLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARA VSVTITEAK VARFL RLKN+ RMKG+NRAAV DCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSRS FD QMGDLTTWVSAALTDEDTCVDGFEGERG++VNLIRNRV+KA+YITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLAASGFETAPN
Sbjct: 181 ALVNKLAASGFETAPN 196

[Close]

BLAST of Lag0002557 vs. NCBI nr
Match: XP_023004663.1 (pectinesterase inhibitor 6 [Cucurbita maxima])

HSP 1 Score: 332.8 bits (852), Expect = 2.0e-87
Identity = 168/196 (85.71%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           MLRAP+SR +I ++LA ++ LPWLTHSA+TSYV+EACSVTLHPDLC++SLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFIAILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARA VSVTITEAK VARFL RLKN+ RMKG+NRAAV DCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSRS FD QMGDLTTWVSAALTDEDTCVDGFEGERG++VNLIRNRV+KA+YITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLAASGFETAPN
Sbjct: 181 ALVNKLAASGFETAPN 196

[Close]

BLAST of Lag0002557 vs. NCBI nr
Match: XP_022960074.1 (pectinesterase inhibitor 6-like [Cucurbita moschata])

HSP 1 Score: 330.9 bits (847), Expect = 7.6e-87
Identity = 167/196 (85.20%), Postives = 182/196 (92.86%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           MLRAP+SR +I ++LA +S LPWLTHSA+TSYV+EACSVTLHPDLC++SLSPFSSTAKRS
Sbjct: 63  MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 122

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARA VSVTITEAK VARFL RLKN+ RMKG+NRAAV DCVEVFE AIDELHRSLGV
Sbjct: 123 PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 182

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSRS FD QMGDLTTWVSAALTDEDTCVDGFEGERG++VNLIRNR +KA+YITSNAL
Sbjct: 183 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRAVKATYITSNAL 242

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLAASGFET PN
Sbjct: 243 ALVNKLAASGFETGPN 258

[Close]

BLAST of Lag0002557 vs. NCBI nr
Match: XP_038896715.1 (pectinesterase inhibitor 6-like [Benincasa hispida])

HSP 1 Score: 328.6 bits (841), Expect = 3.8e-86
Identity = 169/197 (85.79%), Postives = 181/197 (91.88%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           MLRAP++R +IA+ LAL+S LP LTHS KTSYVREACSVT H DLC+QSL+PFSSTAKRS
Sbjct: 1   MLRAPNTRASIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRS 60

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           P KWARAGVSVTITEAKTVA  LGRLKN+ RMKG+NRAAV DCVEVFEAAIDELHRSLGV
Sbjct: 61  PAKWARAGVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSRSNFD Q+GDLTTWVSAALTDEDTCV+GFEGERGK+VNLIRNRVLK  YITSNAL
Sbjct: 121 LRRLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNQ 198
           ALVNKLAASG ETAPNQ
Sbjct: 181 ALVNKLAASGLETAPNQ 197

[Close]

BLAST of Lag0002557 vs. ExPASy Swiss-Prot
Match: O22244 (Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.3e-33
Identity = 76/163 (46.63%), Postives = 108/163 (66.26%), Query Frame = 0


Query: 30  TSYVREACSVTLHPDLCVQSLSPFSSTAKRSPTKWARAGVSVTITEAKTVARFLGRLKNS 89
           ++YVR AC+VT +  LCV++L PF+  A+ + +KWARA V+VTIT+ K V R L + + S
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLLLKTQRS 105

Query: 90  GRMKGKNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRSNFDTQMGDLTTWVSAALTDEDT 149
             +    R A+ DC E+F  ++D L++SL VLR L+   F  Q+ DL TW+SAALTD+DT
Sbjct: 106 A-VGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDDT 165

Query: 150 CVDGFE--GERGKVVNLIRNRVLKASYITSNALALVNKLAASG 191
           C+DGFE    R + V ++R +  K   + SNALAL+ KLA  G
Sbjct: 166 CLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLAFDG 207

[Close]

BLAST of Lag0002557 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.5e-26
Identity = 68/184 (36.96%), Postives = 109/184 (59.24%), Query Frame = 0


Query: 10  AIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRSPTKWARAGV 69
           A+  ILA  SA P         +++ +C++T +P +C QSLS ++ T + +P + A   +
Sbjct: 12  ALLVILAAASAAP------ANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTAL 71

Query: 70  SVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGVLRRLSR--- 129
            V++T  +    F+ RL     +K +  AA+ DC+E  E ++D + RS   ++ LS    
Sbjct: 72  QVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKG 131

Query: 130 SNFDTQMGDLTTWVSAALTDEDTCVDGFEGE--RGKVVNLIRNRVLKASYITSNALALVN 189
           ++F  +M ++ TWVSAALTDE TC+DGF G+   GK+   +R +V+  + +TSNALALVN
Sbjct: 132 NDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVN 189

[Close]

BLAST of Lag0002557 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.3e-25
Identity = 61/170 (35.88%), Postives = 106/170 (62.35%), Query Frame = 0


Query: 25  THSAKTS--YVREACSVTLHPDLCVQSLSPFSSTAKRSPTKWARAGVSVTITEAKTVARF 84
           T+S +T+  Y++ +C++TL+  +C  SLSP++ST + +P K A   +++T++ AK+ ++F
Sbjct: 138 TYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 197

Query: 85  LGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELH---RSLGVLRRLSRSNFDTQMGDLTTW 144
           +  + + G +      AV DCVE    ++  L    R L  +     + F+  M D+ TW
Sbjct: 198 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 257

Query: 145 VSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNALALVNKLAAS 190
           VSAALT++DTC+DGF   +  V +L+R  V++ + +TSNALAL+N  A++
Sbjct: 258 VSAALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALINMYAST 307

[Close]

BLAST of Lag0002557 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.5e-24
Identity = 67/192 (34.90%), Postives = 113/192 (58.85%), Query Frame = 0


Query: 4   APSSRL---AIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 63
           AP+  L   AIA  +   ++     H+     V  +C    +P LCV++LS +S     +
Sbjct: 2   APTQNLFLVAIAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITN 61

Query: 64  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 123
               A+A + ++++ A++ A+ L  +++S   K + +AA++DCVE+   ++DEL R+LGV
Sbjct: 62  RRDLAQAAIKISLSHAQSAAKKLAVVRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGV 121

Query: 124 LRRL-----SRSNFDTQMGDLTTWVSAALTDEDTCVDGFEG-ERGKVVNLIRNRVLKASY 183
           L+ L     S   F  QM +  TW SAALTD+DTC+DGF+G + G++   ++  + K + 
Sbjct: 122 LKHLRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVAR 181

Query: 184 ITSNALALVNKL 187
           +TSNAL +VN+L
Sbjct: 182 VTSNALYMVNQL 193

[Close]

BLAST of Lag0002557 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 8.1e-23
Identity = 60/168 (35.71%), Postives = 95/168 (56.55%), Query Frame = 0


Query: 27  SAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRSPTKWARAGVSVTITEAKTVARFLGRL 86
           S  T +++ +C  T +PD C QSLS ++S  K+ P K A   ++V+I  AK+   ++  +
Sbjct: 32  SGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEM 91

Query: 87  KNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGVLRRL----SRSNFDTQMGDLTTWVSA 146
            +   +  +   AV DC+E     +D L  SL  L+ L    S  +F   + ++ TW SA
Sbjct: 92  TDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSA 151

Query: 147 ALTDEDTCVDGFEGE--RGKVVNLIRNRVLKASYITSNALALVNKLAA 189
           ALTDE  C+DGF G+   G++ +LIR  ++  +  TSNALAL+N  A+
Sbjct: 152 ALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFAS 199

[Close]

BLAST of Lag0002557 vs. ExPASy TrEMBL
Match: A0A6J1L076 (pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 9.7e-88
Identity = 168/196 (85.71%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           MLRAP+SR +I ++LA ++ LPWLTHSA+TSYV+EACSVTLHPDLC++SLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFIAILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARA VSVTITEAK VARFL RLKN+ RMKG+NRAAV DCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSRS FD QMGDLTTWVSAALTDEDTCVDGFEGERG++VNLIRNRV+KA+YITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLAASGFETAPN
Sbjct: 181 ALVNKLAASGFETAPN 196

[Close]

BLAST of Lag0002557 vs. ExPASy TrEMBL
Match: A0A6J1H9V5 (pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111460929 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.7e-87
Identity = 167/196 (85.20%), Postives = 182/196 (92.86%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           MLRAP+SR +I ++LA +S LPWLTHSA+TSYV+EACSVTLHPDLC++SLSPFSSTAKRS
Sbjct: 63  MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 122

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARA VSVTITEAK VARFL RLKN+ RMKG+NRAAV DCVEVFE AIDELHRSLGV
Sbjct: 123 PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 182

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSRS FD QMGDLTTWVSAALTDEDTCVDGFEGERG++VNLIRNR +KA+YITSNAL
Sbjct: 183 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRAVKATYITSNAL 242

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLAASGFET PN
Sbjct: 243 ALVNKLAASGFETGPN 258

[Close]

BLAST of Lag0002557 vs. ExPASy TrEMBL
Match: A0A5D3D1R1 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.4e-86
Identity = 166/196 (84.69%), Postives = 182/196 (92.86%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           M+R PS+R +IA++LAL+S LPWLTHSAKTSYV+EAC VTLH DLC+QSLSPFSSTAKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARAGVSVTITEAK VA  LGRLKN+ RMKG+NRAAVLDCVEVF+AAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSR NFD+QMGDLTTWVSAALTDEDTCV+GFEGERGKVV+L+RNRV+K  YITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLA SGFETA N
Sbjct: 181 ALVNKLATSGFETALN 196

[Close]

BLAST of Lag0002557 vs. ExPASy TrEMBL
Match: A0A1S3BSW5 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.4e-86
Identity = 166/196 (84.69%), Postives = 182/196 (92.86%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           M+R PS+R +IA++LAL+S LPWLTHSAKTSYV+EAC VTLH DLC+QSLSPFSSTAKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARAGVSVTITEAK VA  LGRLKN+ RMKG+NRAAVLDCVEVF+AAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSR NFD+QMGDLTTWVSAALTDEDTCV+GFEGERGKVV+L+RNRV+K  YITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLA SGFETA N
Sbjct: 181 ALVNKLATSGFETALN 196

[Close]

BLAST of Lag0002557 vs. ExPASy TrEMBL
Match: A0A0A0KAX0 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.5e-84
Identity = 164/196 (83.67%), Postives = 175/196 (89.29%), Query Frame = 0


Query: 1   MLRAPSSRLAIASILALVSALPWLTHSAKTSYVREACSVTLHPDLCVQSLSPFSSTAKRS 60
           MLRAPS R +I ++LAL+S LPWLTHSAKTSYV+EAC VT H DLC+QSLSPFSS AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARAGVSVTITEAKTVARFLGRLKNSGRMKGKNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARAGVSVTITEAK VA  LGRLKN+ RMKG+NRAAVLDCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRSNFDTQMGDLTTWVSAALTDEDTCVDGFEGERGKVVNLIRNRVLKASYITSNAL 180
           LRRLSR NFD QMGDLTTWVSAALTDEDTCV+GFEGE GKVV L+RNRV+K  YITSNAL
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLAAS FET  N
Sbjct: 181 ALVNKLAASSFETTIN 196

The following BLAST results are available for this feature:

BLAST of Lag0002557 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6593199.1	2.4e-88	86.29	Pectinesterase inhibitor 6, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023514751.1	9.0e-88	86.22	pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo] >KAG7030218.1 Pectineste...	[more]
XP_023004663.1	2.0e-87	85.71	pectinesterase inhibitor 6 [Cucurbita maxima]	[more]
XP_022960074.1	7.6e-87	85.20	pectinesterase inhibitor 6-like [Cucurbita moschata]	[more]
XP_038896715.1	3.8e-86	85.79	pectinesterase inhibitor 6-like [Benincasa hispida]	[more]

BLAST of Lag0002557 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22244	2.3e-33	46.63	Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1	[more]
P17407	3.5e-26	36.96	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI74	1.3e-25	35.88	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q84WE4	1.5e-24	34.90	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]
Q9SB37	8.1e-23	35.71	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]

BLAST of Lag0002557 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L076	9.7e-88	85.71	pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1	[more]
A0A6J1H9V5	3.7e-87	85.20	pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111460929 PE...	[more]
A0A5D3D1R1	2.4e-86	84.69	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630...	[more]
A0A1S3BSW5	2.4e-86	84.69	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1	[more]
A0A0A0KAX0	8.5e-84	83.67	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..182 e-value: 4.0E-46 score: 169.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..182 e-value: 2.6E-33 score: 115.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 13..186 e-value: 1.8E-34 score: 116.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 27..188 e-value: 7.5E-36 score: 125.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 31..186
None	No IPR available	PANTHER	PTHR31080:SF158	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 18..181
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 18..181
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 36..188 e-value: 7.43768E-46 score: 146.814

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002557.1	Lag0002557.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity