Lag0002546 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002546 vs. NCBI nr
Match: KAA3453480.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 463.4 bits (1191), Expect = 3.1e-126
Identity = 250/618 (40.45%), Postives = 357/618 (57.77%), Query Frame = 0

Query: 1   MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVS 60
           MK  CWN RG+G+PRAVR LR+ +++H+P +VFL ETK      D+++R  GF N INV 
Sbjct: 1   MKHFCWNARGLGSPRAVRRLRYILKQHHPHLVFLMETKLDSKCMDRVRRSCGFTNGINVE 60

Query: 61  SRGNSGGLALLWQNQHHITVNSYSKGHIDVTVKEP--EWWWRFTGFYGNPDQGKRKDSWR 120
           + G+ GGL L W++   +T+ SYSK HIDV +KE   +  WRFTGFYG+P    +K  W 
Sbjct: 61  AEGSRGGLCLAWRDDIGVTLRSYSKWHIDVLIKEEGIQEEWRFTGFYGSPYVRDQKYVWN 120

Query: 121 LLERLHDSINLPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSGDR 180
           LLERL    + PW++ GDFNEIMF+ EK+GG  +    +  F D ++ C L+DVGFSG  
Sbjct: 121 LLERLSQDKDYPWLVAGDFNEIMFSFEKQGGQLRDPRRMETFRDTLANCGLMDVGFSGTW 180

Query: 181 YTWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYGQQ 240
           +TW R    +   +ERLDR   N          R++HL F  SDH P++L    +N    
Sbjct: 181 FTWERGNLSETNIRERLDRGVANEKWLTLFPMGRIQHLPFIISDHCPLLLTTDSDN---- 240

Query: 241 RLYTKKSIRFEDWWTQNEGSKLSFKEAWDQSDEASTINFNSKIQEGLMAMSTWNK--ERL 300
            +   +   FE WWT  E  +   +  W+ S E        K++   + +  W K  +R 
Sbjct: 241 TIIRNRRFHFETWWTMEESFEGVLRNIWESSSEP----LIQKLKTLQVGLEQWAKTLKRK 300

Query: 301 KGSIKNAIIRKEREINTLVKSKSPDNFTNLIQAEKELEKLLEEEECYWKIRSREDWLKSE 360
           KG +K  +      +  L K +  +    LI  +  L   +++EE YW+ RSR +WL+  
Sbjct: 301 KGVLKKKLTEDLESL--LGKDRDDETLAKLIDTKIRLNLEIDKEERYWEQRSRVNWLQFG 360

Query: 361 DRNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRESIQ 420
           DRNT +FH  A+ RK+ N I  ++   G  +  +  +   A  +   L  S   +    +
Sbjct: 361 DRNTAFFHKSATTRKKVNLITKLVLDEGLEITDETGLQEAAKSYFENLFTSEGVA--DPK 420

Query: 421 GSIEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGED 480
             +E I+S IS+E    L  PF ++E+   +K M  LKAPG DG  A  +Q  W  +G++
Sbjct: 421 KVLEGIESCISQEVNDVLISPFKEDEVYAALKGMRPLKAPGSDGFPALFFQKYWHIVGKE 480

Query: 481 TVKICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALANRMK 540
            ++ CLGILN+ + +E  N T I LIPK + P ++  +RPISLC V YK+V K +ANR++
Sbjct: 481 VLEYCLGILNDGKEIEAANMTDIVLIPKVSQPTALVNFRPISLCTVVYKLVTKTIANRLQ 540

Query: 541 KVLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVEW 600
            V+   I + QSAF+PG  I+DNVI+ +E LHT   KR GK G++A+KLDMSKAYDRVEW
Sbjct: 541 SVIGGCIDKAQSAFIPGLLISDNVILAYELLHTYRQKRMGKKGYMAVKLDMSKAYDRVEW 600

Query: 601 SFVEEIMRKMNFSERWTR 615
            FV ++MRKM FS  W R
Sbjct: 601 DFVIQVMRKMGFSAIWIR 606

BLAST of Lag0002546 vs. NCBI nr
Match: XP_042956310.1 (uncharacterized protein LOC122292152 [Carya illinoinensis])

HSP 1 Score: 462.6 bits (1189), Expect = 5.3e-126
Identity = 249/619 (40.23%), Postives = 368/619 (59.45%), Query Frame = 0

Query: 1   MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVS 60
           M+ L WN RG+GNPR+V  L   ++   P +VFL ETK  K   ++I + L F +C+ + 
Sbjct: 1   MRCLSWNCRGLGNPRSVNILNMLVKDKCPNLVFLMETKCSKSRMEEICKLLKFESCLVID 60

Query: 61  SRGNSGGLALLWQNQHHITVNSYSKGHIDVTV----KEPEWWWRFTGFYGNPDQGKRKDS 120
           S G+SGGL L+W N+ ++++ +YS+ HI   +    + P   W FTGFYG+P+  KR  S
Sbjct: 61  SVGSSGGLTLMWNNKVNVSIFNYSRWHISAQIIPNLETPP--WLFTGFYGHPETAKRHMS 120

Query: 121 WRLLERLHDSINLPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSG 180
           W LL  L    ++ W+  GDFNE++   EK+GG P+P   +  F  AI  C+L  +   G
Sbjct: 121 WELLRSLKPHPSMAWLCCGDFNELLCQNEKQGGCPRPYKQMETFRHAIMDCSLNPIQTQG 180

Query: 181 DRYTWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYG 240
             +TWS N+ E E TKERLDR   N            + +    SDH P+++ +  EN  
Sbjct: 181 PFFTWSNNRKEDEFTKERLDRAMANNNWMHCFTESYCQVIPAIKSDHSPLLIHV--ENTA 240

Query: 241 QQRLYTKKSIRFEDWWTQNEGSKLSFKEAWDQ---SDEASTINFNSKIQEGLMAMSTWNK 300
            +R       RFE  W   +G     +EAW +    D+A T + + +I    +A+  W K
Sbjct: 241 SRRPCNTHLFRFEAAWNMKDGISNIIREAWVKVAVGDDAVT-SMHKRILNCSLALKRW-K 300

Query: 301 ERLKGSIKNAIIRKEREINTLVKSKSPDNFTNLIQAEKELEKLLEEEECYWKIRSREDWL 360
                     I RK +E+  L  +   D    +   +KE+E+ L EEE  WK R+++ WL
Sbjct: 301 GAASNKATGDIKRKMKELEVLQDTNQGDQVEAITLLQKEIEQSLGEEELKWKQRAKQHWL 360

Query: 361 KSEDRNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRE 420
           ++ DRNTK++H  ASQR++ N++  I D+N   V + ++IG + T    +L  S++P   
Sbjct: 361 QNGDRNTKFYHLHASQRRKTNKVSQIYDSNDNLVTQKQQIGEVFTTHFVDLFTSSNPC-- 420

Query: 421 SIQGSIEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTI 480
            I   +E I +K++    + L  PF++EE++  V  M  L +PGPDG  A  YQS W+TI
Sbjct: 421 DIGRCLEDIPTKVTPLMNEVLLQPFSEEEVKSAVFQMKALGSPGPDGFPAIFYQSHWETI 480

Query: 481 GEDTVKICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALAN 540
           G++     L ++N+  SLE +N+T ITLIPK  DPK + ++RPISLCNV YKIVAK L+N
Sbjct: 481 GKEVTSFALNVVNDGGSLEGVNETFITLIPKTKDPKRVPDFRPISLCNVIYKIVAKMLSN 540

Query: 541 RMKKVLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDR 600
           R+K VL  IIS NQSAFVPGR ITDN+++ +E LHT++ + +GK+G++A+KLDMSKAYDR
Sbjct: 541 RLKIVLPDIISPNQSAFVPGRSITDNILVAYETLHTMSTRMKGKSGYMALKLDMSKAYDR 600

Query: 601 VEWSFVEEIMRKMNFSERW 613
           VEWSF+  +M ++ F + W
Sbjct: 601 VEWSFLYSVMSRLGFHQNW 611

BLAST of Lag0002546 vs. NCBI nr
Match: XP_042958247.1 (uncharacterized protein LOC122293873 [Carya illinoinensis])

HSP 1 Score: 462.2 bits (1188), Expect = 6.9e-126
Identity = 249/619 (40.23%), Postives = 368/619 (59.45%), Query Frame = 0

Query: 1   MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVS 60
           M+ L WN RG+GNPR+V  L   ++   P +VFL ETK  K   ++I + L F +C+ + 
Sbjct: 1   MRCLSWNCRGLGNPRSVNILNMLVKDKCPNLVFLMETKCSKSRMEEICKLLKFESCLVID 60

Query: 61  SRGNSGGLALLWQNQHHITVNSYSKGHIDVTV----KEPEWWWRFTGFYGNPDQGKRKDS 120
           S G+SGGL L+W N+ ++++ +YS+ HI   +    + P   W FTGFYG+P+  KR  S
Sbjct: 61  SVGSSGGLTLMWNNKVNVSIFNYSRWHISAQIIPNLETPP--WLFTGFYGHPETAKRHMS 120

Query: 121 WRLLERLHDSINLPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSG 180
           W LL  L    ++ W+  GDFNE++   EK+GG P+P   +  F  AI  C+L  +   G
Sbjct: 121 WELLRSLKPHPSMAWLCCGDFNELLCQNEKQGGCPRPYKQMETFRHAIMDCSLNPIQTQG 180

Query: 181 DRYTWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYG 240
             +TWS N+ E E TKERLDR   N            + +    SDH P+++ +  EN  
Sbjct: 181 PFFTWSNNRKEDEFTKERLDRAMANNNWMHCFTESYCQVIPAIKSDHSPLLIHV--ENTA 240

Query: 241 QQRLYTKKSIRFEDWWTQNEGSKLSFKEAW---DQSDEASTINFNSKIQEGLMAMSTWNK 300
            +R       RFE  W   +G     +EAW      D+A+T + + +I    +A+  W K
Sbjct: 241 SRRPCNTHLFRFEAAWNVKDGISNIIREAWVKVAMGDDAAT-SMHKRILNCSLALKRW-K 300

Query: 301 ERLKGSIKNAIIRKEREINTLVKSKSPDNFTNLIQAEKELEKLLEEEECYWKIRSREDWL 360
                     I RK +E+  L  +   D    +   +KE+E+ L EEE  WK R+++ WL
Sbjct: 301 GAASNKATGDIKRKMKELEILQDTNQGDQVEAITLLQKEIEQSLGEEELKWKQRAKQHWL 360

Query: 361 KSEDRNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRE 420
           ++ DRNTK++H  ASQR++ N++  I D+N   V + ++IG + T    +L  S++P   
Sbjct: 361 QNGDRNTKFYHLHASQRRKTNKVSQIYDSNDNLVTQKQQIGEVFTTHFVDLFTSSNPC-- 420

Query: 421 SIQGSIEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTI 480
            I   +E I +K++    + L  PF++EE++  V  M  L +PGPDG  A  YQS W+TI
Sbjct: 421 DIGRCLEDIPTKVTPLMNEVLLQPFSEEEVKSAVFQMKALGSPGPDGFPAIFYQSHWETI 480

Query: 481 GEDTVKICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALAN 540
           G++     L ++N+  SLE +N+T ITLIPK  DPK + ++RPISLCNV YKIVAK L+N
Sbjct: 481 GKEVTSFALNVVNDGGSLEGVNETFITLIPKTKDPKRVPDFRPISLCNVIYKIVAKMLSN 540

Query: 541 RMKKVLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDR 600
           R+K VL  IIS NQSAFVPGR ITDN+++ +E LHT++ + +GK+G++A+KLDMSKAYDR
Sbjct: 541 RLKIVLPDIISPNQSAFVPGRSITDNILVAYETLHTMSTRMQGKSGYMALKLDMSKAYDR 600

Query: 601 VEWSFVEEIMRKMNFSERW 613
           VEWSF+  +M ++ F + W
Sbjct: 601 VEWSFLYSVMSRLGFHQNW 611

BLAST of Lag0002546 vs. NCBI nr
Match: XP_042974832.1 (uncharacterized protein LOC122306468 [Carya illinoinensis])

HSP 1 Score: 451.8 bits (1161), Expect = 9.3e-123
Identity = 235/615 (38.21%), Postives = 377/615 (61.30%), Query Frame = 0

Query: 1   MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVS 60
           MKIL WN RG+GNPR VR L   +++ +PT++FL ETK  K+  +++   LG++ C+ V 
Sbjct: 1   MKILAWNCRGLGNPRTVRYLNLLVKEKSPTMIFLMETKCMKVRIEEVCNMLGYDGCLVVD 60

Query: 61  SRGNSGGLALLWQNQHHITVNSYSKGHIDVTV-KEPEWWWRFTGFYGNPDQGKRKDSWRL 120
           SRG+SGGL  LW+ +  +++ +YS+ HI   V    E  W FTGFYGNP+  KR  SW  
Sbjct: 61  SRGSSGGLTFLWKTRDKVSIYNYSRWHISAMVGMGMEQPWLFTGFYGNPETSKRHLSWDF 120

Query: 121 LERLHDSINLPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSGDRY 180
           L+ +    + PW+  GDFNEI+   EK GG P+P + +  F + + +C+L  +   G  +
Sbjct: 121 LKSIKPDDSTPWMCCGDFNEIVCQDEKMGGGPRPYNQMEIFRNTLEWCSLSSLVTKGPCF 180

Query: 181 TWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYGQQR 240
           TW+ N+ +    KERLDR   NP   ++ +      L    SDH P+++++ ++   Q R
Sbjct: 181 TWANNREQDSFIKERLDRALANPMWLERYQESSCCVLPTMTSDHCPVLIDVIYQQ-DQPR 240

Query: 241 LYTKKSIRFEDWWTQNEGSKLSFKEAWDQ--SDEASTINFNSKIQEGLMAMSTWNKERLK 300
            + K+  R+E  W+  +  + + KEAW +   + A+     S++      +  WN++ ++
Sbjct: 241 PHVKR-FRYEAAWSLRKECEATLKEAWVKPIHERAAAETIRSRLTMSATVLRKWNEKVIR 300

Query: 301 GSIKNAIIRKEREINTLVKSKSPDNFTNLIQAEKELEKLLEEEECYWKIRSREDWLKSED 360
            + K  I +K + +  L +    D   ++ + + ++++ L  E+  WK R+++ WL++ D
Sbjct: 301 KAPKE-IRQKLKALEELQELNKGDQLDSIRKLKGDIDQDLAAEDLKWKQRAKQHWLQNGD 360

Query: 361 RNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRESIQG 420
           RNT ++H  AS R++ N+I  I+D     + + +++GR+ T++  +L  S+ PS   I+ 
Sbjct: 361 RNTTFYHLHASHRRKINKISQIMDHRNCLISEKDQVGRVITRYFSDLFTSSSPS--GIED 420

Query: 421 SIEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGEDT 480
            I+ + +K+S    + L   FT+EE++  V  M  + +PGPDG  A  +Q+ W+T G++ 
Sbjct: 421 CIKDMPNKVSTAMNELLLQEFTEEEVKNAVFQMKSMGSPGPDGFPACFFQTHWNTTGKEV 480

Query: 481 VKICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALANRMKK 540
            +  L ILN   SLE +N T ITLIPK  +P  +S+YRPISLCNV YK+VAK L+NR+K 
Sbjct: 481 SRFVLNILNTAGSLEGVNDTYITLIPKIKNPTKVSDYRPISLCNVIYKVVAKVLSNRLKL 540

Query: 541 VLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVEWS 600
           +L  IIS NQSAFVPGR ITDN+++ +E LH+++ + +GK+ ++A+KLDMSKAYDRVEW 
Sbjct: 541 ILPDIISVNQSAFVPGRAITDNIMVAYETLHSMSTRIQGKSAYMALKLDMSKAYDRVEWD 600

Query: 601 FVEEIMRKMNFSERW 613
           F+E +M KM F  RW
Sbjct: 601 FLEVVMCKMGFDRRW 610

BLAST of Lag0002546 vs. NCBI nr
Match: XP_030936391.1 (uncharacterized protein LOC115961572 [Quercus lobata])

HSP 1 Score: 449.5 bits (1155), Expect = 4.6e-122
Identity = 247/613 (40.29%), Postives = 356/613 (58.08%), Query Frame = 0

Query: 1   MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVS 60
           M IL WN RG+GN R V++L   + K  P +VFL ETK+ +   +K+K +    + + VS
Sbjct: 1   MSILSWNCRGLGNLRTVKALERVVTKEEPKVVFLMETKSKREWLEKVKDRCKMKHGLTVS 60

Query: 61  SRGNSGGLALLWQNQHHITVNSYSKGHIDVTVKEPEW---WWRFTGFYGNPDQGKRKDSW 120
           S G+ GGLALLW+    + +N+Y++ HID  + E  W    W FTGFYGNPD  +R +SW
Sbjct: 61  SDGSKGGLALLWKEGITVKINTYAQDHIDAWI-EGGWDGVSWHFTGFYGNPDTAQRPESW 120

Query: 121 RLLERLHDSINLPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSGD 180
             L+ L  + ++PW+  GDFNEI    EK+GG  +P   + +F DAI+YC   +V F G 
Sbjct: 121 AKLKSLKGTTSVPWLAIGDFNEITGLTEKEGGRVRPRRQMENFVDAINYCGFREVDFIGP 180

Query: 181 RYTWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYGQ 240
           +YTW  ++ +    +ERLDR   N          ++ HL+   SDH P+ L +  +   +
Sbjct: 181 KYTWWYHRADGMHIRERLDRALANKEWMDLFPAAKLYHLSSSASDHSPLSLHLVPK---R 240

Query: 241 QRLYTKKSIRFEDWWTQNEGSKLSFKEAWDQSDEASTIN-FNSKIQEGLMAMSTWNKERL 300
           ++   +KS RFE  W ++   +   K AW+  + +       S ++     +  WNKE  
Sbjct: 241 KKKKIRKSFRFESMWLKDSRCEEIVKAAWEIGEHSGAEGILKSCLEHCRHDLEKWNKEEF 300

Query: 301 KGSIKNAIIRKEREINTLVKSKSPDNFTNLIQAEK-ELEKLLEEEECYWKIRSREDWLKS 360
            G +   I   ++++  L    S       ++  +  L K LE+E+  W+ RSR +W + 
Sbjct: 301 -GHVGRKISELQQKLEWLELQPSSSGILGELRTTRVNLNKWLEKEDEMWRQRSRLNWFQG 360

Query: 361 EDRNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRESI 420
            DRNT +FHAKAS R +KN I GI+D  G+W E + +I  +A  +  +L  S+ P  E  
Sbjct: 361 GDRNTSFFHAKASARHQKNYIDGIVDEQGRWQEDELKIEEVAVAYFEKLFTSSKP--EEF 420

Query: 421 QGSIEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGE 480
              + A+  K++ +    L   +T +E+   +K M  LKAPGPDG     +Q  W+T GE
Sbjct: 421 SDILHAVQPKVTTDMNVELTREYTAQEVRLALKQMYPLKAPGPDGMPPLFFQHFWNTCGE 480

Query: 481 DTVKICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALANRM 540
                 L  LN+  S    N+T I LIPK N+PK +S+YRPISLCNV+YKI +KA+ANR+
Sbjct: 481 VVTSTVLDFLNHGMSPPNFNETHIVLIPKINEPKHVSDYRPISLCNVTYKIASKAIANRL 540

Query: 541 KKVLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVE 600
           KK L SIIS  QSAFV GR ITDNV++ FE +H ++ K+ GK G +AIKLDMSKAYDRVE
Sbjct: 541 KKFLPSIISDTQSAFVHGRLITDNVLVAFETMHHISRKKGGKVGEMAIKLDMSKAYDRVE 600

Query: 601 WSFVEEIMRKMNF 609
           W FVE+IM K+ F
Sbjct: 601 WVFVEKIMEKLGF 606

BLAST of Lag0002546 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 2.5e-30
Identity = 123/494 (24.90%), Postives = 232/494 (46.96%), Query Frame = 0

Query: 132 IIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSGD----RYTWSRNKYE 191
           IIGGDFN  + +   +    K  S  +   + I++ +L+DV    +     +T+ R + +
Sbjct: 139 IIGGDFNYTL-DARDRNVPKKRDSSESVLRELIAHFSLVDVWREQNPETVAFTYVRVR-D 198

Query: 192 KEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYGQQRLYTKKSIR 251
              ++ R+DR + +  +  +++   +    F  SDH  + L +       +  Y      
Sbjct: 199 GHVSQSRIDRIYISSHLMSRAQSSTIRLAPF--SDHNCVSLRMSIAPSLPKAAYW----H 258

Query: 252 FEDWWTQNEGSKLSFKEAWDQ----SDEASTINFNSKIQEGLMAMSTWNKERLKGSIKNA 311
           F +   ++EG   S ++ W       DE +T+N    + +  + +      +     +NA
Sbjct: 259 FNNSLLEDEGFAKSVRDTWRGWRAFQDEFATLNQWWDVGKVHLKLLCQEYTKSVSGQRNA 318

Query: 312 IIRKEREINTLVKSKSPDNFTNLIQAE----KELEKLLEEEECYWK-IRSREDWLKSEDR 371
            I         ++ +   +    +Q E    KE  + +E+ +     +RSR   L   DR
Sbjct: 319 EIEALNGEVLDLEQRLSGSEDQALQCEYLERKEALRNMEQRQARGAFVRSRMQLLCDMDR 378

Query: 372 NTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRESIQGS 431
            +++F+A   ++  + +I  +   +G  +E  E I   A  F   L + +  S ++ +  
Sbjct: 379 GSRFFYALEKKKGNRKQITCLFAEDGTPLEDPEAIRDRARSFYQNLFSPDPISPDACEEL 438

Query: 432 IEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGEDTV 491
            + +   +SE +K+RL+ P T +E+ Q ++ M   K+PG DG   + +Q  WDT+G D  
Sbjct: 439 WDGL-PVVSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFH 498

Query: 492 KICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALANRMKKV 551
           ++        E      + +++L+PKK D + +  +RP+SL +  YKIVAKA++ R+K V
Sbjct: 499 RVLTEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSV 558

Query: 552 LDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVEWSF 611
           L  +I  +QS  VPGR I DNV +  + LH     RR       + LD  KA+DRV+  +
Sbjct: 559 LAEVIHPDQSYTVPGRTIFDNVFLIRDLLHF---ARRTGLSLAFLSLDQEKAFDRVDHQY 618

Query: 612 VEEIMRKMNFSERW 613
           +   ++  +F  ++
Sbjct: 619 LIGTLQAYSFGPQF 620

BLAST of Lag0002546 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.2e-27
Identity = 140/630 (22.22%), Postives = 269/630 (42.70%), Query Frame = 0

Query: 3   ILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVSSR 62
           IL  NV G+ +P     L   I+  +P++  + ET        ++K + G+      + +
Sbjct: 10  ILTLNVNGLNSPIKRHRLASWIKSQDPSVCCIQETHLTCRDTHRLKIK-GWRKIYQANGK 69

Query: 63  GNSGGLALLWQNQHHI---TVNSYSKGH---IDVTVKEPEWWWRFTGFYGNPDQGKRKDS 122
               G+A+L  ++       +    +GH   +  ++++ E        Y  P+ G  +  
Sbjct: 70  QKKAGVAILVSDKTDFKPTKIKRDKEGHYIMVKGSIQQEE--LTILNIYA-PNTGAPRFI 129

Query: 123 WRLLERLHDSINLPWIIGGDFNE--IMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGF 182
            ++L  L   ++   +I GDFN    + ++  +    K    +N    A+   +LID+  
Sbjct: 130 KQVLSDLQRDLDSHTLIMGDFNTPLSILDRSTRQKVNKDTQELN---SALHQTDLIDIYR 189

Query: 183 S----GDRYTWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEI 242
           +       YT+    +    T  ++D    +  +   SK +R E +  + SDH  I LE+
Sbjct: 190 TLHPKSTEYTFFSAPHH---TYSKIDHIVGSKAL--LSKCKRTEIITNYLSDHSAIKLEL 249

Query: 243 GWENYGQQRLYTKK--SIRFEDWWTQNEGSKLSFKEAWDQSDEASTI------NFNSKIQ 302
             +N  Q R  T K  ++   D+W  NE  K   K  ++ ++   T        F +  +
Sbjct: 250 RIKNLTQSRSTTWKLNNLLLNDYWVHNE-MKAEIKMFFETNENKDTTYQNLWDAFKAVCR 309

Query: 303 EGLMAMSTWNKERLKGSI---KNAIIRKEREINTLVKSKSPDNFTNLIQAEKELEKLLEE 362
              +A++ + +++ +  I    + +   E++  T  K+      T +    KE    +E 
Sbjct: 310 GKFIALNAYKRKQERSKIDTLTSQLKELEKQEQTHSKASRRQEITKIRAELKE----IET 369

Query: 363 EECYWKIRSREDWLKSEDRNTKWFHAKASQRKR-KNEIKGILDTNGKWVEKDEEIGRIAT 422
           ++   KI     W            A+  ++KR KN+I  I +  G       EI     
Sbjct: 370 QKTLQKINESRSWFFERINKIDRPLARLIKKKREKNQIDTIKNDKGDITTDPTEIQTTIR 429

Query: 423 KFIHELLNSNHPSRESIQGSIEAID-SKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPG 482
           ++   L  +   + E +   ++     ++++E+ + L+ P T  EI  ++ ++   K+PG
Sbjct: 430 EYYKHLYANKLENLEEMDTFLDTYTLPRLNQEEVESLNRPITGSEIVAIINSLPTKKSPG 489

Query: 483 PDGAHAKLYQSLWDTIGEDTVKICLGILNNNESLEPMNKTLITLIPKK-NDPKSMSEYRP 542
           PDG  A+ YQ   + +    +K+   I           +  I LIPK   D      +RP
Sbjct: 490 PDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTKKENFRP 549

Query: 543 ISLCNVSYKIVAKALANRMKKVLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRG 602
           ISL N+  KI+ K LANR+++ +  +I  +Q  F+PG Q   N+      +  +N  R  
Sbjct: 550 ISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQHIN--RAK 609

Query: 603 KTGHVAIKLDMSKAYDRVEWSFVEEIMRKM 607
              HV I +D  KA+D+++  F+ + + K+
Sbjct: 610 DKNHVIISIDAEKAFDKIQQPFMLKTLNKL 620

BLAST of Lag0002546 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.3e-26
Identity = 151/645 (23.41%), Postives = 268/645 (41.55%), Query Frame = 0

Query: 1   MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQL-GFNNCINV 60
           + I   NV G+  P     L   I+K  P I  + E  ++    DK + ++ G+++    
Sbjct: 7   LSIFSINVNGLNCPLKRHRLADWIQKLKPDICCIQE--SHLTLKDKYRLKVKGWSSIFQA 66

Query: 61  SSRGNSGGLALLWQNQHHITVNSYSK---GHIDVTVKEPEWWWRFT--GFYGNPDQGKRK 120
           + +    G+A+L+ +          K   GH  + VK    +   +    Y  P+    +
Sbjct: 67  NGKQKKAGIAILFADAIGFKPTKIRKDKDGHF-IFVKGNTQYDEISIINIYA-PNHNAPQ 126

Query: 121 DSWRLLERLHDSINLPWIIGGDFN---EIMFNKEKKGGTPKPVSLINDFCDAISYCNLID 180
                L  + + I+   I+ GDFN    ++    KK    K    I D    I + +L D
Sbjct: 127 FIRETLTDMSNLISSTSIVVGDFNTPLAVLDRSSKK----KLSKEILDLNSTIQHLDLTD 186

Query: 181 VGFSGDRYTWSRNKYE------KEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRP 240
           +       T+  NK E         T  ++D    + +    SKF+++E +    SDH  
Sbjct: 187 I-----YRTFHPNKTEYTFFSSAHGTYSKIDHILGHKS--NLSKFKKIEIIPCIFSDHHG 246

Query: 241 IVLEIGWENYGQQRLYTK----KSIRFEDWWT-------------QNEGSKLSFKEAWDQ 300
           I +E+   N      +TK     ++  +D W              QN     +++  WD 
Sbjct: 247 IKVEL--NNNRNLHTHTKTWKLNNLMLKDTWVIDEIKKEITKFLEQNNNQDTNYQNLWDT 306

Query: 301 SDEASTINFNSKIQEGLMAMSTWNKERLKGSIKNAIIRKEREINTLVKSKSPDNFTNLIQ 360
           +       F + +Q  L          L G +K   + KE   N       P     + +
Sbjct: 307 AKAVLRGKFIA-LQAFLKKTEREEVNNLMGHLKQ--LEKEEHSN-----PKPSRRKEITK 366

Query: 361 AEKELEKLLEEEECYWKIRSREDWLKSEDRNTKWFHAKASQRKR-KNEIKGILDTNGKWV 420
              EL + +E +    +I   + W   +        A  +++KR K+ I  I + N +  
Sbjct: 367 IRAELNE-IENKRIIQQINKSKSWFFEKINKIDKPLANLTRKKRVKSLISSIRNGNDEIT 426

Query: 421 EKDEEIGRIATKFIHELLNSNHPSRESIQGSIEAID-SKISEEQKQRLDGPFTKEEIEQV 480
               EI +I  ++  +L +  + + + I   +EA    ++S+++ + L+ P +  EI   
Sbjct: 427 TDPSEIQKILNEYYKKLYSHKYENLKEIDQYLEACHLPRLSQKEVEMLNRPISSSEIAST 486

Query: 481 VKNMNLLKAPGPDGAHAKLYQSLWDTIGEDTVKICLGILNNNESLEPMNKTL----ITLI 540
           ++N+   K+PGPDG  ++ YQ    T  E+ V I L +  N E    +  T     ITLI
Sbjct: 487 IQNLPKKKSPGPDGFTSEFYQ----TFKEELVPILLNLFQNIEKEGILPNTFYEANITLI 546

Query: 541 PKK-NDPKSMSEYRPISLCNVSYKIVAKALANRMKKVLDSIISQNQSAFVPGRQITDNVI 600
           PK   DP     YRPISL N+  KI+ K L NR+++ +  II  +Q  F+PG Q   N+ 
Sbjct: 547 PKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQVGFIPGSQGWFNIR 606

Query: 601 MGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVEWSFVEEIMRKM 607
                +  +N  +     H+ + +D  KA+D ++  F+   ++K+
Sbjct: 607 KSINVIQHINKLK--NKDHMILSIDAEKAFDNIQHPFMIRTLKKI 619

BLAST of Lag0002546 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 102.4 bits (254), Expect = 1.8e-20
Identity = 95/378 (25.13%), Postives = 179/378 (47.35%), Query Frame = 0

Query: 243 KKSIRFEDWWTQNEGSKLSFKEAWDQSDEASTINFNSKIQEGLMAMSTWNKERLKGSIKN 302
           KK I+  D+   NE    ++   WD           + ++  L+A+S   K+R      +
Sbjct: 274 KKEIK--DFLEFNENEATTYPNLWD--------TMKAFLRGKLIALSASKKKRETAHTSS 333

Query: 303 AIIR----KEREINTLVKSKSPD-----NFTNLIQAEKELEKLLEEEECYWKIRSREDWL 362
                   +++E N+  +S+  +        N ++  + ++++ +    +++  ++ D  
Sbjct: 334 LTTHLKALEKKEANSPKRSRRQEIIKLRGEINQVETRRTIQRINQTRSWFFEKINKID-- 393

Query: 363 KSEDRNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRE 422
           K   R TK    K    K +NE KG + T+       EEI      F   L ++   + +
Sbjct: 394 KPLARLTKGHRDKILINKIRNE-KGDITTD------PEEIQNTIRSFYKRLYSTKLENLD 453

Query: 423 SIQGSIEAID-SKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDT 482
            +   ++     K++++Q   L+ P + +EIE V+ ++   K+PGPDG  A+ YQ    T
Sbjct: 454 EMDKFLDRYQVPKLNQDQVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQ----T 513

Query: 483 IGEDTVKICLGILNNNESLEPMNKTL----ITLIPK-KNDPKSMSEYRPISLCNVSYKIV 542
             ED + I   + +  E    +  +     ITLIPK + DP  +  +RPISL N+  KI+
Sbjct: 514 FKEDLIPILHKLFHKIEVEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKIL 573

Query: 543 AKALANRMKKVLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDM 602
            K LANR+++ + +II  +Q  F+PG Q   N+      +H +N  +     H+ I LD 
Sbjct: 574 NKILANRIQEHIKAIIHPDQVGFIPGMQGWFNIRKSINVIHYINKLK--DKNHMIISLDA 626

Query: 603 SKAYDRVEWSFVEEIMRK 606
            KA+D+++  F+ +++ +
Sbjct: 634 EKAFDKIQHPFMIKVLER 626

BLAST of Lag0002546 vs. ExPASy TrEMBL
Match: A0A803PBM9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 7.4e-134
Identity = 265/618 (42.88%), Postives = 368/618 (59.55%), Query Frame = 0

Query: 1    MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVS 60
            MK+L WNV+G+GNP  VR+L+  + + +P +VF++E++  K  A+ ++  LG++ C  V 
Sbjct: 427  MKLLLWNVQGLGNPWTVRTLKSLVNRWDPELVFISESRLKKGKAEALRVSLGYDGCFVVE 486

Query: 61   SRGNSGGLALLWQNQHHITVNSYSKGHIDVTV-KEPEWWWRFTGFYGNPDQGKRKDSWRL 120
            + G SGGL LLW N     + S+S  HID  + KE   WWRFTGFYG+PD  +R +SW+L
Sbjct: 487  AHGKSGGLILLWSNMVDCNIMSFSSFHIDSFIRKEEGQWWRFTGFYGDPDPTQRCESWKL 546

Query: 121  LERLHDSINLPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSGDRY 180
            L R+    + PW+IGGDFNEI+ NKEK GG PKP  LIN+F  A++  NL +V + G  Y
Sbjct: 547  LARIGRMYSGPWVIGGDFNEILRNKEKLGGQPKPGYLINNFRMALNGSNLREVEYEGSEY 606

Query: 181  TWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYGQQR 240
            TW  N  + E   ERLDR   NP         +V HL+   SDH P++L    +N+  ++
Sbjct: 607  TWC-NGRKNELIFERLDRVCGNPEWFDLFPKAKVNHLDRVSSDHCPLLLHCLVKNHENEK 666

Query: 241  -LYTKKSIRFEDWWTQNEGSKLSFKEAWDQSDEAST-INFNSKIQEGLMAMSTWNKERLK 300
             +       FE  W   E      KE+WD+    +T +    K+     A+  WNK R K
Sbjct: 667  GVRWHSRFHFEHAWADEEKCTEIVKESWDKGGSCNTAMALRDKLACCGKALQKWNKSR-K 726

Query: 301  GSIKNAIIRKEREINTLVKSKSPDNFTNLIQAEKELEKLLEEEECYWKIRSREDWLKSED 360
              +K  +   E +I  L +S +  ++  L   E++   LL++EE +W+ RSR  WLK  D
Sbjct: 727  TEMKQRLKEYEEKITILSRSTNNKDWQYLKDLEQKNNVLLDKEEKFWRQRSRAIWLKEGD 786

Query: 361  RNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRESIQG 420
            RNTK+FH KA+ RKRKN I G+LD+N KWV  ++ +G++A  +  ++  SN  S   ++ 
Sbjct: 787  RNTKYFHRKANTRKRKNTILGLLDSNEKWVHGNKMVGQVACLYFQQIFTSNSASIADLEE 846

Query: 421  SIEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGEDT 480
                + +KIS E  + L  PFTKE++   ++N++  KAPG DG     Y+  W  IGE+ 
Sbjct: 847  FQRIVPNKISRETNEYLKAPFTKEDVYAAMRNIHPHKAPGSDGMPGLFYRLYWPKIGEEV 906

Query: 481  VKICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALANRMKK 540
             K+CLGILN    L  +N TLI LIPK   P  M+ +RPISLCNV YKIVAK LA R K 
Sbjct: 907  TKVCLGILNEGRPLNEINDTLICLIPKIEKPTRMANFRPISLCNVIYKIVAKCLAGRRKH 966

Query: 541  VLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVEWS 600
             L   IS+ QSAFV GR I DN I+GFE LH +  +R G    +A+KLDMSKAYDRVEW 
Sbjct: 967  SLAQAISEEQSAFVGGRLIQDNAIIGFESLHCMKKRRFGNRRKMALKLDMSKAYDRVEWG 1026

Query: 601  FVEEIMRKMNFSERWTRK 616
            F+  +MR + + E W  K
Sbjct: 1027 FLITMMRGLGYEEDWIEK 1042

BLAST of Lag0002546 vs. ExPASy TrEMBL
Match: A0A803PCN1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 2.4e-132
Identity = 256/609 (42.04%), Postives = 363/609 (59.61%), Query Frame = 0

Query: 10   GMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVSSRGNSGGLA 69
            G+GNP  ++SL   ++ H+P ++FLAET+  +   ++I+   GF++C  V+++G SGGLA
Sbjct: 885  GLGNPWTLKSLCSHVKDHHPGMIFLAETRLNEAAMERIRIIFGFDSCFVVAAKGKSGGLA 944

Query: 70   LLWQNQHHITVNSYSKGHIDVTVKEP-EWWWRFTGFYGNPDQGKRKDSWRLLERLHDSIN 129
            LLW++   IT+NS++  HID  V+    ++WRFTGFYG+PD G RK SW L+ERL D   
Sbjct: 945  LLWKDSWEITINSFTVSHIDAKVENGLGFFWRFTGFYGSPDPGGRKHSWLLMERLRDMRQ 1004

Query: 130  LPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSGDRYTWSRNKYEK 189
             PWI GGDFNEIM  KEKKGG  K  S I +F  AISYCN  ++   G+ +TW  N  + 
Sbjct: 1005 GPWICGGDFNEIMREKEKKGGCLKQASQIREFQKAISYCNFKEIKMEGE-FTWC-NGRQN 1064

Query: 190  EATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEI-GWENYGQQRLYTKKSIR 249
                E+LDR F NP   +K    +V  L + +SDHRP++L         +  L  K    
Sbjct: 1065 NLVFEKLDRVFANPEWIRKFGESKVSLLPWWNSDHRPLILNFRNKTQTSKSDLKWKSRFH 1124

Query: 250  FEDWWTQNEGSKLSFKEAW-DQSDEASTINFNSKIQEGLMAMSTWNKERLKGSIKNAIIR 309
            +E  W   E         W D S+  +      +I      ++ WNK + K  +     +
Sbjct: 1125 YEQAWASEEECGKIVNNVWLDGSNWGTAKGIRGRINHCGEVLNEWNKGK-KAELGAKTKK 1184

Query: 310  KEREINTLVKSKSPDNFTNLIQAEKELEKLLEEEECYWKIRSREDWLKSEDRNTKWFHAK 369
             ++E+N L  S    N+ N  + EKEL     +EE  WK RSR  WL   DRNTK+FH K
Sbjct: 1185 LKKELNYLSSSSGETNWENRRRVEKELNVAEAKEELLWKQRSRALWLTHGDRNTKYFHYK 1244

Query: 370  ASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRESIQGSIEAIDSKI 429
            ASQRK+KN+I G+ D N KW  K+EEI  I      +L +S+ P++  +      + +++
Sbjct: 1245 ASQRKKKNKINGLFDDNMKWCNKEEEIEHILIHSYTDLFSSSRPNQNKMDILNRCVPNRV 1304

Query: 430  SEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGEDTVKICLGILN 489
            S +    L   FTKEE+++ +  ++ LKAPG DG     Y + W+ +G + +  CL +LN
Sbjct: 1305 SSQDNDMLMKDFTKEEVKEALDQIHPLKAPGKDGLPGLFYHNHWEEVGHEVIATCLEVLN 1364

Query: 490  NNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALANRMKKVLDSIISQN 549
            NN     +N TL+ LIPK  DP  +S++RP+SLCNV YK ++K LANRMK  ++ +IS+N
Sbjct: 1365 NNMDCSCLNDTLLCLIPKIKDPTKVSDFRPLSLCNVIYKAISKCLANRMKLSMEKVISEN 1424

Query: 550  QSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVEWSFVEEIMRKM 609
            QSAF+ GRQI DN I+GFE LH + N R G    +A+KLDMSKAYDRVEW F+EE+MR +
Sbjct: 1425 QSAFIKGRQIQDNAIIGFESLHCMKNGRFGNGKKMALKLDMSKAYDRVEWDFIEEMMRCL 1484

Query: 610  NFSERWTRK 616
             + E+W  K
Sbjct: 1485 GYEEQWISK 1490

BLAST of Lag0002546 vs. ExPASy TrEMBL
Match: A0A7N2LIH6 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 2.7e-128
Identity = 257/615 (41.79%), Postives = 357/615 (58.05%), Query Frame = 0

Query: 1    MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVS 60
            M IL WN RG+G   AVR+L  E++K NP +VFL ETK         + +LGF   I V 
Sbjct: 690  MNILAWNCRGLGTSPAVRTLTDEVKKKNPVLVFLVETKASVEKMKGFQNKLGFTQGIIVP 749

Query: 61   SRGNSGGLALLWQNQHHITVNSYSKGHIDVTVKEPEWW--WRFTGFYGNPDQGKRKDSWR 120
            S G SGGLALLW+    I   S S  HIDV V        WR TGFYG+PD GKR  SW+
Sbjct: 750  SDGRSGGLALLWKEGTDIRFKSCSHSHIDVVVHGAGSGGPWRATGFYGHPDTGKRYTSWK 809

Query: 121  LLERLHDSINLPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSGDR 180
            LLE L+    +PW++ GDFNEI+   EK G   +  + ++ F + +S C LID+GF G R
Sbjct: 810  LLEILNTQCEMPWLVCGDFNEIVHPDEKMGWKDRDAAQMDAFREVLSKCGLIDLGFVGPR 869

Query: 181  YTWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYGQQ 240
            +TW   ++  + T  RLDR   N          +V H++   SDH  + L +   N  Q+
Sbjct: 870  FTWCNGRFGDQRTLIRLDRMVANEAWSLMFPEAKVHHVSMSASDHCLLALFLNKVN-NQR 929

Query: 241  RLYTKKSIRFEDWWTQNEGSKLSFKEAWDQSDEASTINFNSKIQEGLMAMSTWNKERLKG 300
            R   KK   FE+ WT+ E  K   + AWD   E S +    +++     +  WN+    G
Sbjct: 930  R--GKKRFFFEEMWTRVEECKEIVELAWDPYREDSAMPVQERLERCQKMLQQWNQNSF-G 989

Query: 301  SIKNAIIRKEREINTLVKSKSPDNFTNLIQA-EKELEKLLEEEECYWKIRSREDWLKSED 360
            ++   I +K+  +  L            IQ  +KE+ +L   EE  WK RSR  WL+  D
Sbjct: 990  NVYKGIKQKKNRLQQLESLNLLHETAEEIQTLKKEINELHTREEVMWKQRSRVSWLQYGD 1049

Query: 361  RNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRESIQG 420
            +N+K+FHA ASQR++KN I G++D  G W E  E   ++   +  ++ +SN P+  S   
Sbjct: 1050 KNSKFFHATASQRRQKNRIGGLMDDLGVWHEDQETTEKLILDYFKDIYSSNQPT--SFDV 1109

Query: 421  SIEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGEDT 480
            S+EA+D +++ E    L   F   E+ Q ++ M+  KAPGPDG     YQ  WD +G   
Sbjct: 1110 SLEAMDERVTPEMNDELQKEFKAVEVWQALQQMHPTKAPGPDGMSPIFYQKYWDIVGSSV 1169

Query: 481  VKICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALANRMKK 540
                L  LN+    + +NKT I LIPK  +P+ ++E+RPISLCNV YKI++K LANR+KK
Sbjct: 1170 TNCVLQALNSGVMPKDINKTYICLIPKTKNPQKITEFRPISLCNVIYKIISKVLANRLKK 1229

Query: 541  VLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVEWS 600
            VL  +I + QSAFVPGR ITDNVI+ FE +H++N +R+GK G +AIKLDMSKAYDRVEW+
Sbjct: 1230 VLHGVIDEAQSAFVPGRMITDNVIVAFESMHSINQRRKGKEGLMAIKLDMSKAYDRVEWA 1289

Query: 601  FVEEIMRKMNFSERW 613
            ++E +M+KM F +RW
Sbjct: 1290 YLESMMKKMGFGDRW 1298

BLAST of Lag0002546 vs. ExPASy TrEMBL
Match: A0A2N9IPS8 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55418 PE=4 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 3.6e-128
Identity = 249/619 (40.23%), Postives = 377/619 (60.90%), Query Frame = 0

Query: 1    MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVS 60
            M++L WN +G+GN   VR L   I++ +PT++FL+ET+  K+  ++++  + F+    V 
Sbjct: 441  MRLLSWNCQGLGNLCTVRELLLLIKEQDPTVLFLSETRLDKIGVERLRVTIKFDGAFCVP 500

Query: 61   SRGNSGGLALLWQNQHHITVNSYSKGHIDVTV--KEPEWWWRFTGFYGNPDQGKRKDSWR 120
             RG  GGLA+LW  +  + + +YS+ HID  +  KE    +R TGFYGNP+  KRK+SW 
Sbjct: 501  RRGTGGGLAVLWIAKLDVKLTTYSRNHIDAEIVDKEKGKGFRLTGFYGNPETHKRKESWA 560

Query: 121  LLERLHDSINLPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSGDR 180
            LL+ L    + PW+  GDFNEI+ N E+ G   +P   I DF +A+ +C L D+G+ G+ 
Sbjct: 561  LLKHLSHLSSSPWLCMGDFNEILDNNERLGSGHRPEWQIRDFREAVVHCGLHDLGYVGNS 620

Query: 181  YTWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYGQQ 240
            YTW R +        RLDR   + +         V HL   +SDH PI+L+I     G  
Sbjct: 621  YTWRRKRDGNALVTARLDRMMASVSWLTDYDGSVVSHLAVQNSDHCPILLDI---PDGPL 680

Query: 241  RLYTKKSIRFEDWWTQNEGSKLSFKEAWDQSDEASTINFN--SKIQEGLMAMSTWNKERL 300
                KK  RFE  W ++E  +     AW       +  F    K++    ++  W++ER 
Sbjct: 681  VKRKKKLFRFEAMWIKDEQCREVIDHAWGDGVTEGSPMFMVVEKMKLCRTSLIGWSRERF 740

Query: 301  KGSIKNAIIRKEREINTLVKSKSPDNF-TNLIQAEKELEKLLEEEECYWKIRSREDWLKS 360
             GS+ ++I RK  ++  L+ +++P  F T +++ + +L  LLE+EE +W+ RSR  W+  
Sbjct: 741  -GSLASSIKRKREQLQHLI-NETPSGFSTGILELQDDLNGLLEKEEIFWRQRSRVAWMSE 800

Query: 361  EDRNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRESI 420
             D+NTK+FHA+ ++R+R N I G+ D +G W  +  +I  IA  +   +  S++PS ESI
Sbjct: 801  GDKNTKFFHAQCNERRRTNHISGLRDRDGVWQTEKTKIAEIAVDYFQGIFTSSNPSAESI 860

Query: 421  QGSIEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGE 480
               ++ ++S ++     +L   FTK+E+   +K M   KAPGPDG  A  YQ+ WD +G 
Sbjct: 861  TTVLQGMESVVTNAMNDQLQAEFTKDEVSLALKQMYPTKAPGPDGMSAIFYQTYWDIVGP 920

Query: 481  DTVKICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALANRM 540
            +  +  L IL++   L  +N T I LIPK  +P++++++RPISLCNV YKIV+K LANR+
Sbjct: 921  EVTQAILSILHSGYMLRKINYTHIALIPKVKNPENITDFRPISLCNVIYKIVSKVLANRL 980

Query: 541  KKVLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVE 600
            KKVL  +IS+ QSAFVPGR ITDNV++ FE +H+++ KR+GK G +A+KLDMSKAYDRVE
Sbjct: 981  KKVLPFVISEAQSAFVPGRLITDNVLVAFEVMHSMSLKRKGKKGQMALKLDMSKAYDRVE 1040

Query: 601  WSFVEEIMRKMNFSERWTR 615
            W F+E IMR M F++ W R
Sbjct: 1041 WVFLESIMRGMGFAKEWIR 1054

BLAST of Lag0002546 vs. ExPASy TrEMBL
Match: A0A803QQ69 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 1.0e-127
Identity = 248/618 (40.13%), Postives = 366/618 (59.22%), Query Frame = 0

Query: 1    MKILCWNVRGMGNPRAVRSLRHEIRKHNPTIVFLAETKNYKLPADKIKRQLGFNNCINVS 60
            M IL WNV+G+GNP  +++L   ++ ++P ++FL+ET+   +  ++I+  LGF+ C  V+
Sbjct: 413  MSILIWNVQGLGNPWTLKALCSHVKDYHPEMIFLSETRLNDVAMERIRVVLGFDGCFVVA 472

Query: 61   SRGNSGGLALLWQNQHHITVNSYSKGHIDVTVKEP-EWWWRFTGFYGNPDQGKRKDSWRL 120
            ++G SGGLALLW+    + V S++  HID  V+    + WRFTGFYG+PD G RKDSW L
Sbjct: 473  AKGKSGGLALLWKEPFEVNVKSFTVSHIDAMVENGLGFSWRFTGFYGSPDPGGRKDSWLL 532

Query: 121  LERLHDSINLPWIIGGDFNEIMFNKEKKGGTPKPVSLINDFCDAISYCNLIDVGFSGDRY 180
            LERL D +   W+ GGDFNEI+  KEKKGG  K  +L+ DF  AISYCN  ++  +G  +
Sbjct: 533  LERLRDMLQGAWVCGGDFNEIIKAKEKKGGCVKNEALLRDFRRAISYCNFKEITMNGGEF 592

Query: 181  TWSRNKYEKEATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYGQQR 240
            TW  N  +     E+LDR   NPT  +      V  L +  SDHRP++L+         R
Sbjct: 593  TWC-NGRQDNLVFEKLDRVLANPTWFKYFCESSVTLLPWWSSDHRPMLLKFSLAMDRANR 652

Query: 241  LYTKKS-IRFEDWWTQNEGSKLSFKEAW-DQSDEASTINFNSKIQEGLMAMSTWNKERLK 300
            + T +S   +E  W + E      +  W D ++  S      +I      +   NK++ K
Sbjct: 653  MPTWRSRFHYEQAWGEEEECGRIVESVWFDNTNWGSPHGLRGRINLCGETLKDCNKDK-K 712

Query: 301  GSIKNAIIRKEREINTLVKSKSPDNFTNLIQAEKELEKLLEEEECYWKIRSREDWLKSED 360
              ++    R + E+N L KS    ++    + E EL     ++E  WK RSR  WL   D
Sbjct: 713  ADLRARTKRLKEELNNLSKSSRAADWKTRRRIEGELNVAETKDEIMWKQRSRALWLAHGD 772

Query: 361  RNTKWFHAKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHPSRESIQG 420
            RNTK+FH KASQRK+KN IKG+ D + +W ++D EI  I  K+  +L  ++ P  +    
Sbjct: 773  RNTKYFHHKASQRKKKNMIKGLFDDHRRWCKEDHEIEAILVKYYSDLFKTSDPIPQMRDF 832

Query: 421  SIEAIDSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGEDT 480
                + +++S +  + L   FT EE+++ +  ++ LKAPG DG     Y + W  +G++ 
Sbjct: 833  LSRCVPNQMSMQDNENLMADFTIEEVKEAIDQIHPLKAPGKDGLPGLFYHNHWKEVGQEV 892

Query: 481  VKICLGILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIVAKALANRMKK 540
            + +CL +LN N+    +N TL+ LIPK  +P  + +YRP+SLCNV YK+++K LANRMK 
Sbjct: 893  MTMCLDVLNKNKDCSILNDTLLCLIPKVKEPTMVGDYRPLSLCNVIYKVISKCLANRMKL 952

Query: 541  VLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKAYDRVEWS 600
             +D +IS+NQSAF+ GRQI DN I+GFE LH L   R G    +A+KLDMSKAYDRVEW 
Sbjct: 953  SMDKVISENQSAFIKGRQIQDNAILGFESLHCLKKGRFGNGKKMALKLDMSKAYDRVEWD 1012

Query: 601  FVEEIMRKMNFSERWTRK 616
            F+ E+M+ + + +RW  K
Sbjct: 1013 FLAEMMKCLGYDDRWIAK 1028

BLAST of Lag0002546 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 1.0e-18
Identity = 96/405 (23.70%), Postives = 170/405 (41.98%), Query Frame = 0

Query: 132 IIGGDFNEIMFNKEKKG--GTPKPVSLINDFCDAISYCNLIDVGFSGDRYTWSRNKYEKE 191
           I+ GDF++I    +      T  P+  + +F + +   +L+D+   G  YTWS N  +  
Sbjct: 222 ILVGDFDQIAATSDHYSVLQTSIPMRGLEEFQNCLRDSDLVDIPSRGVHYTWS-NHQDDN 281

Query: 192 ATKERLDRFFFNPTMEQKSKFRRVEHLNFHHSDHRPIVLEIGWENYGQQRLYTKKSIRFE 251
               +LDR   N                   SDH P ++ +  EN  ++   +KK  R+ 
Sbjct: 282 PIIRKLDRAIANGDWFSSFPSAIAVFELSGVSDHSPCIIIL--ENLPKR---SKKCFRYF 341

Query: 252 DWWTQNEGSKLSFKEAWDQSDEASTINFN--SKIQEGLMAMSTWNKE---RLKGSIKNAI 311
            + + +    +S   AW++     +  F+    ++         N++    ++   K A+
Sbjct: 342 SFLSTHPTFLVSLTVAWEEQIPVGSHMFSLGEHLKAAKKCCKLLNRQGFGNIQHKTKEAL 401

Query: 312 IRKEREINTLVKSKSPDNFTNLIQAEKELEKLLEEEECYWKIRSREDWLKSEDRNTKWFH 371
              E   + L+ + S   F     A K+        E +++ +SR  WL+  D NT++FH
Sbjct: 402 DSLESIQSQLLTNPSDSLFRVEHVARKKWNFFAAALESFYRQKSRIKWLQDGDANTRFFH 461

Query: 372 AKASQRKRKNEIKGILDTNGKWVEKDEEIGRIATKFIHELLNSNHP--SRESIQGSIEAI 431
                 + KN IK +   +   VE   ++  +   +   LL S+    + +S+Q   +  
Sbjct: 462 KVILANQAKNLIKFLRMDDDVRVENVTQVKEMIVAYYTHLLGSDSDILTPDSVQRIKDIH 521

Query: 432 DSKISEEQKQRLDGPFTKEEIEQVVKNMNLLKAPGPDGAHAKLYQSLWDTIGEDTVKICL 491
             + ++    RL    + +EI   V  M   KAPGPD   A+ +   W  + + T+    
Sbjct: 522 PFRCNDTLASRLSALPSDKEITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVK 581

Query: 492 GILNNNESLEPMNKTLITLIPKKNDPKSMSEYRPISLCNVSYKIV 528
                   L+  N T ITLIPK      +S +RP+S C V YKI+
Sbjct: 582 EFFRTGHLLKRFNATAITLIPKVTGVDQLSMFRPVSCCTVVYKII 620

BLAST of Lag0002546 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 70.9 bits (172), Expect = 4.1e-12
Identity = 29/82 (35.37%), Postives = 52/82 (63.41%), Query Frame = 0

Query: 531 LANRMKKVLDSIISQNQSAFVPGRQITDNVIMGFECLHTLNNKRRGKTGHVAIKLDMSKA 590
           +  R+K ++ ++I   Q++F+PGR  TDN++   E +H++  K +G  G + +KLD+ KA
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRK-KGVKGWMLLKLDLEKA 60

Query: 591 YDRVEWSFVEEIMRKMNFSERW 613
           YDR+ W ++E+ +    F E W
Sbjct: 61  YDRIRWDYLEDTLISAGFPEVW 81

BLAST of Lag0002546 vs. TAIR 10
Match: AT1G40390.1 (DNAse I-like superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 4.9e-05
Identity = 29/97 (29.90%), Postives = 47/97 (48.45%), Query Frame = 0

Query: 110 QGKRKDSWRLLERLHDS---INLPWIIGGDFNEIMFNKEKKGGTPKPVSL--INDFCDAI 169
           + +R+  W  + RL  S    N PW++ GDFN+I    E     P  +SL  + D    +
Sbjct: 98  EAERRSLWDDITRLSASSPLCNSPWLVVGDFNQIASVTEHYSLMPSNISLQGLEDLQACM 157

Query: 170 SYCNLIDVGFSGDRYTWSRNKYEKEATKERLDRFFFN 202
              +L+D+   G  YTWS ++ +    + +LDR   N
Sbjct: 158 RDSDLVDLPCRGVLYTWSNHQQDNPILR-KLDRAIVN 193