Lag0002531 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002531
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr4: 43629194 .. 43630576 (-)
RNA-Seq ExpressionLag0002531
SyntenyLag0002531
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACACCTTTTGCCGCACAATTCTGTGCTCAATCAAACAAAATTCTCTCTCTTTTCACCGCTTCTCTTTCCCCAATTCACATCCCTCAAATTCAAGCTCAACTCATCGTTCAAAACCTCCATTCAAACCCCACCATAGCCCACCACTTCATCAACACTTGCCACTCTCTTCAACTTCTCGATTCCGCTCTTCTCTTCTTCACCCATTTCCCCAAACCCCACGTCTTCATCTGCAATTCCTTAATCAGAGCCTTCTCTCACTCCAAAATCCCCCATACCCCTCTTTCCATTTACGCCCACATGAACAGAAACTCCATTCTTCCCAACAATTACACCTTCCCTTTCCTTCTCAAGTCCTTGGCTGATTTCGGCGACCTTGTCAGTGGACAATCCGTTCACACCCATGTTCTGAGATTGGGATATGTTTCTGATGTTTATGTGCAGAACTCGGTGATGGACGTTTATGCGTCGTGTGGCAGAATGGGGCTATGCAAGAAGGTGTTCGACGAAATGCCCCAAAGAGATGTTGTGTCGTGGACTGTTTTGATCATGGGTTATCGATGTGCTTTGATGTTTGATGATGCTTTGATTGCGTTTGAGCAGATGCAATATGCGGGTGTGGAGCCTAACCGTGTGACGATGGTGAATGCATTGGCTGCCTGTGCGAGCTTTGGAGCTGTTGAAATGGGTGTTTGGATACATGAGTTTGTGAAGAGGAGAGGGTGGGAAGTGGATGTGATTTTGGGGACTTCTTTGATTGATATGTATGGGAAATGTGGGAGAATCAAAGAGGGATTGGTTGTTTTCCAAGCCATGAAAGAGAAGAATGTGTATACATGGAATGCACTCATTAAAGGGCTGGCATTGGCCAAGAGTGGAGAGGAGGCCATTGCTTGGTTTAAGAAAATGGATAATGAAGGAGTTGAGGCAGATGAGGTGACAGTGCTGGCAGTTCTTTGCGCTTGTAGCCACTCTGGCTTGGTGGACATGGGGAGGCAGATCTTTCGATCGTTGTTCGATGGGAGGTTCGGGTTTTCTCCGGGAATCAAACATTATTCATGTATGGTTGATCTCTTGGCTCGTTATGGGTGTATTGAAGAGGCTTTTGAATTGATAAAGGATATGCCTTTTGATGCCACCAAAGCAATGTGGGGATCTTTGCTAGCTGGTGCCAGAGCTCATGGGAGCTTGGAAGTGAGCGAATTCGCAGCGAAGAAGCTTGTCGAAATGGAGCCAATGAATGGTGCTTATTATGTTGTGTTATCTAACATTTGTGCAGAGATGGGAAGATGGAGTGAGGTTGAGAAAGTGAGAGAGATCATGAAAGAGAGAGGACTGAAGAAGGATTTAGGGTCGAGTTCTGTTGAAGAAACTGTTTTCTGA

mRNA sequence

ATGACACCTTTTGCCGCACAATTCTGTGCTCAATCAAACAAAATTCTCTCTCTTTTCACCGCTTCTCTTTCCCCAATTCACATCCCTCAAATTCAAGCTCAACTCATCGTTCAAAACCTCCATTCAAACCCCACCATAGCCCACCACTTCATCAACACTTGCCACTCTCTTCAACTTCTCGATTCCGCTCTTCTCTTCTTCACCCATTTCCCCAAACCCCACGTCTTCATCTGCAATTCCTTAATCAGAGCCTTCTCTCACTCCAAAATCCCCCATACCCCTCTTTCCATTTACGCCCACATGAACAGAAACTCCATTCTTCCCAACAATTACACCTTCCCTTTCCTTCTCAAGTCCTTGGCTGATTTCGGCGACCTTGTCAGTGGACAATCCGTTCACACCCATGTTCTGAGATTGGGATATGTTTCTGATGTTTATGTGCAGAACTCGGTGATGGACGTTTATGCGTCGTGTGGCAGAATGGGGCTATGCAAGAAGGTGTTCGACGAAATGCCCCAAAGAGATGTTGTGTCGTGGACTGTTTTGATCATGGGTTATCGATGTGCTTTGATGTTTGATGATGCTTTGATTGCGTTTGAGCAGATGCAATATGCGGGTGTGGAGCCTAACCGTGTGACGATGGTGAATGCATTGGCTGCCTGTGCGAGCTTTGGAGCTGTTGAAATGGGTGTTTGGATACATGAGTTTGTGAAGAGGAGAGGGTGGGAAGTGGATGTGATTTTGGGGACTTCTTTGATTGATATGTATGGGAAATGTGGGAGAATCAAAGAGGGATTGGTTGTTTTCCAAGCCATGAAAGAGAAGAATGTGTATACATGGAATGCACTCATTAAAGGGCTGGCATTGGCCAAGAGTGGAGAGGAGGCCATTGCTTGGTTTAAGAAAATGGATAATGAAGGAGTTGAGGCAGATGAGGTGACAGTGCTGGCAGTTCTTTGCGCTTGTAGCCACTCTGGCTTGGTGGACATGGGGAGGCAGATCTTTCGATCGTTGTTCGATGGGAGGTTCGGGTTTTCTCCGGGAATCAAACATTATTCATGTATGGTTGATCTCTTGGCTCGTTATGGGTGTATTGAAGAGGCTTTTGAATTGATAAAGGATATGCCTTTTGATGCCACCAAAGCAATGTGGGGATCTTTGCTAGCTGGTGCCAGAGCTCATGGGAGCTTGGAAGTGAGCGAATTCGCAGCGAAGAAGCTTGTCGAAATGGAGCCAATGAATGGTGCTTATTATGTTGTGTTATCTAACATTTGTGCAGAGATGGGAAGATGGAGTGAGGTTGAGAAAGTGAGAGAGATCATGAAAGAGAGAGGACTGAAGAAGGATTTAGGGTCGAGTTCTGTTGAAGAAACTGTTTTCTGA

Coding sequence (CDS)

ATGACACCTTTTGCCGCACAATTCTGTGCTCAATCAAACAAAATTCTCTCTCTTTTCACCGCTTCTCTTTCCCCAATTCACATCCCTCAAATTCAAGCTCAACTCATCGTTCAAAACCTCCATTCAAACCCCACCATAGCCCACCACTTCATCAACACTTGCCACTCTCTTCAACTTCTCGATTCCGCTCTTCTCTTCTTCACCCATTTCCCCAAACCCCACGTCTTCATCTGCAATTCCTTAATCAGAGCCTTCTCTCACTCCAAAATCCCCCATACCCCTCTTTCCATTTACGCCCACATGAACAGAAACTCCATTCTTCCCAACAATTACACCTTCCCTTTCCTTCTCAAGTCCTTGGCTGATTTCGGCGACCTTGTCAGTGGACAATCCGTTCACACCCATGTTCTGAGATTGGGATATGTTTCTGATGTTTATGTGCAGAACTCGGTGATGGACGTTTATGCGTCGTGTGGCAGAATGGGGCTATGCAAGAAGGTGTTCGACGAAATGCCCCAAAGAGATGTTGTGTCGTGGACTGTTTTGATCATGGGTTATCGATGTGCTTTGATGTTTGATGATGCTTTGATTGCGTTTGAGCAGATGCAATATGCGGGTGTGGAGCCTAACCGTGTGACGATGGTGAATGCATTGGCTGCCTGTGCGAGCTTTGGAGCTGTTGAAATGGGTGTTTGGATACATGAGTTTGTGAAGAGGAGAGGGTGGGAAGTGGATGTGATTTTGGGGACTTCTTTGATTGATATGTATGGGAAATGTGGGAGAATCAAAGAGGGATTGGTTGTTTTCCAAGCCATGAAAGAGAAGAATGTGTATACATGGAATGCACTCATTAAAGGGCTGGCATTGGCCAAGAGTGGAGAGGAGGCCATTGCTTGGTTTAAGAAAATGGATAATGAAGGAGTTGAGGCAGATGAGGTGACAGTGCTGGCAGTTCTTTGCGCTTGTAGCCACTCTGGCTTGGTGGACATGGGGAGGCAGATCTTTCGATCGTTGTTCGATGGGAGGTTCGGGTTTTCTCCGGGAATCAAACATTATTCATGTATGGTTGATCTCTTGGCTCGTTATGGGTGTATTGAAGAGGCTTTTGAATTGATAAAGGATATGCCTTTTGATGCCACCAAAGCAATGTGGGGATCTTTGCTAGCTGGTGCCAGAGCTCATGGGAGCTTGGAAGTGAGCGAATTCGCAGCGAAGAAGCTTGTCGAAATGGAGCCAATGAATGGTGCTTATTATGTTGTGTTATCTAACATTTGTGCAGAGATGGGAAGATGGAGTGAGGTTGAGAAAGTGAGAGAGATCATGAAAGAGAGAGGACTGAAGAAGGATTTAGGGTCGAGTTCTGTTGAAGAAACTGTTTTCTGA

Protein sequence

MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLLDSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWTVLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRRGWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWFKKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVEETVF
Homology
BLAST of Lag0002531 vs. NCBI nr
Match: XP_038898992.1 (pentatricopeptide repeat-containing protein At5g56310-like [Benincasa hispida])

HSP 1 Score: 846.7 bits (2186), Expect = 9.7e-242
Identity = 411/456 (90.13%), Postives = 434/456 (95.18%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           MTPF AQF A+SN+ILS+FT SLSPIHIPQIQAQLI+QNLHSNPTIAHHFINTCH LQLL
Sbjct: 1   MTPFTAQFRAESNRILSVFTTSLSPIHIPQIQAQLILQNLHSNPTIAHHFINTCHHLQLL 60

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSALLFF H PKPHVF+CNSLIRAFSHSKIPHTPLSIY HMNRNSI PNNYTFPFLLKSL
Sbjct: 61  DSALLFFNHIPKPHVFVCNSLIRAFSHSKIPHTPLSIYTHMNRNSIFPNNYTFPFLLKSL 120

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           ADF DLVSGQSVHTHVL+LGYV+DVYVQNS+MDVYASCG+MGLCKKVFDEMPQRDVVSWT
Sbjct: 121 ADFNDLVSGQSVHTHVLKLGYVADVYVQNSLMDVYASCGKMGLCKKVFDEMPQRDVVSWT 180

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           VLIMGYR +LMFDDALIAFEQMQYAGVEPNRVTMVNALAACA+FGA+EMGVWIHEFVKR+
Sbjct: 181 VLIMGYRVSLMFDDALIAFEQMQYAGVEPNRVTMVNALAACANFGAIEMGVWIHEFVKRK 240

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
           GWE+DVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF
Sbjct: 241 GWEMDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300

Query: 301 KKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLLA 360
            +MD EGVE DEVT++AVLCACSHSGLVDMG+QIF+SL D RFGFSPGIKHYSCMVDLLA
Sbjct: 301 NRMDEEGVEPDEVTLVAVLCACSHSGLVDMGKQIFQSLTDRRFGFSPGIKHYSCMVDLLA 360

Query: 361 RYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYVV 420
           RYGCIE AF LIKDMPF+ATKAMWGSLLAG+RAHG LEVSE AAKKLVEMEP NGAYYVV
Sbjct: 361 RYGCIEAAFVLIKDMPFEATKAMWGSLLAGSRAHGGLEVSEIAAKKLVEMEPENGAYYVV 420

Query: 421 LSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           LSNI AEM +WSEVEKVRE+MKERGLKKDLGSSSVE
Sbjct: 421 LSNIYAEMEKWSEVEKVREMMKERGLKKDLGSSSVE 456

BLAST of Lag0002531 vs. NCBI nr
Match: XP_023548659.1 (pentatricopeptide repeat-containing protein At5g56310-like [Cucurbita pepo subsp. pepo] >XP_023548660.1 pentatricopeptide repeat-containing protein At5g56310-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.1 bits (2130), Expect = 3.0e-235
Identity = 401/457 (87.75%), Postives = 430/457 (94.09%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           M  F  Q  A+SN+ILSLF  SLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCH L+LL
Sbjct: 1   MAAFGVQMWAESNRILSLFATSLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHDLRLL 60

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSALLFFT  PKPHVF+CNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL
Sbjct: 61  DSALLFFTQIPKPHVFVCNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           ADF  LVSGQSVH HV++LGYVSDVYVQNS+MDVYASCGRMGLCKKVFDEMP RDVVSWT
Sbjct: 121 ADFNHLVSGQSVHAHVVKLGYVSDVYVQNSLMDVYASCGRMGLCKKVFDEMPLRDVVSWT 180

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           VLIMGYR ALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGA+EMG+WIHEFVKRR
Sbjct: 181 VLIMGYRVALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAIEMGIWIHEFVKRR 240

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
           GWE+DVILGTSLIDMYGKCGRI+EGLVVFQAMK+KNVYTWNALI GLALAKSGEEAIAWF
Sbjct: 241 GWEMDVILGTSLIDMYGKCGRIEEGLVVFQAMKDKNVYTWNALINGLALAKSGEEAIAWF 300

Query: 301 KKMDNE-GVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLL 360
           K+MD + GV+ADEVT++AVLCACSHSGLVD+GRQIF S+ DG+FGFSPGI+HYSCMVDLL
Sbjct: 301 KRMDEDGGVKADEVTLVAVLCACSHSGLVDIGRQIFGSMIDGKFGFSPGIQHYSCMVDLL 360

Query: 361 ARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYV 420
           AR GCIEE+FELIK+MPFDATKAMWGSLLAG+RA GSLE+SEFAA+KLVEMEP NGAYY 
Sbjct: 361 ARSGCIEESFELIKNMPFDATKAMWGSLLAGSRAQGSLEMSEFAARKLVEMEPENGAYYA 420

Query: 421 VLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           VLSNICAEMG+W+EVEKVR+IMK  GLKKDLGSSSVE
Sbjct: 421 VLSNICAEMGKWNEVEKVRKIMKVEGLKKDLGSSSVE 457

BLAST of Lag0002531 vs. NCBI nr
Match: KAG6575842.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014378.1 Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 822.0 bits (2122), Expect = 2.6e-234
Identity = 401/457 (87.75%), Postives = 428/457 (93.65%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           M  F  Q  A+SN+ILSLF  SLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCH L+LL
Sbjct: 1   MAAFGVQMWAESNRILSLFATSLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHDLRLL 60

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSAL FFT  PKPHVF+CNSLIRAFSHSKIP TPLSIYAHMNRNSILPNNYTFPFLLKSL
Sbjct: 61  DSALRFFTQIPKPHVFVCNSLIRAFSHSKIPRTPLSIYAHMNRNSILPNNYTFPFLLKSL 120

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           ADF  LVSGQSVH HV++LGYVSDVYVQNS+MDVYASCGRMGLCKKVFDEMP RDVVSWT
Sbjct: 121 ADFNHLVSGQSVHAHVVKLGYVSDVYVQNSLMDVYASCGRMGLCKKVFDEMPLRDVVSWT 180

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           VLIMGYR ALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGA+EMGVWIHEFVKRR
Sbjct: 181 VLIMGYRVALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAIEMGVWIHEFVKRR 240

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
           GWE+DVILGTSLIDMYGKCGRI+EGLVVFQAMK+KNVYTWNALI GLALAKSGEEAIAWF
Sbjct: 241 GWEMDVILGTSLIDMYGKCGRIEEGLVVFQAMKDKNVYTWNALINGLALAKSGEEAIAWF 300

Query: 301 KKMDNE-GVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLL 360
           K+M+ E G+EADEVT++AVLCACSHSGLVD+GRQIF S+ DG+FGFSPGI+HYSCMVDLL
Sbjct: 301 KRMNEEGGIEADEVTLVAVLCACSHSGLVDIGRQIFGSMIDGKFGFSPGIQHYSCMVDLL 360

Query: 361 ARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYV 420
           AR GCIEE+FELIK+MPFDATKAMWGSLLAG+RA GSLE+SEFAA+KLVEMEP NGAYY 
Sbjct: 361 ARSGCIEESFELIKNMPFDATKAMWGSLLAGSRAQGSLEMSEFAARKLVEMEPENGAYYA 420

Query: 421 VLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           VLSNICAEMG+W+EVEKVREIMK  GLKKDLGSSSVE
Sbjct: 421 VLSNICAEMGKWNEVEKVREIMKVEGLKKDLGSSSVE 457

BLAST of Lag0002531 vs. NCBI nr
Match: XP_022991568.1 (pentatricopeptide repeat-containing protein At5g56310-like [Cucurbita maxima])

HSP 1 Score: 818.9 bits (2114), Expect = 2.2e-233
Identity = 400/457 (87.53%), Postives = 425/457 (93.00%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           M  F  Q  A+SN+ILSLF  SLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCH L+LL
Sbjct: 1   MAAFGVQMWAESNRILSLFATSLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHHLRLL 60

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSAL FFT  PKPHVF+CNSLIRAFSHSKIPHTPLSIYAHMNR SILPNNYTFPFLLKSL
Sbjct: 61  DSALRFFTQIPKPHVFVCNSLIRAFSHSKIPHTPLSIYAHMNRTSILPNNYTFPFLLKSL 120

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           ADF  LVSGQSVH HV++LGYVSDVYVQNS+MDVYASCGRMGLCKKVFDEMP RDVVSWT
Sbjct: 121 ADFNHLVSGQSVHAHVVKLGYVSDVYVQNSLMDVYASCGRMGLCKKVFDEMPLRDVVSWT 180

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           VLIMGYR ALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGA+EMGVWIH FVKRR
Sbjct: 181 VLIMGYRVALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAIEMGVWIHAFVKRR 240

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
           GWE+DVILGTSLIDMYGKCGRI+EGLVVFQAMK+KNVYTWNALI GLALAKSGEEAIAWF
Sbjct: 241 GWEMDVILGTSLIDMYGKCGRIEEGLVVFQAMKDKNVYTWNALINGLALAKSGEEAIAWF 300

Query: 301 KKMD-NEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLL 360
           K+MD   GVEADEVT++ VLCACSHSGLVD+GRQIF S+ DG+FGFSPGI+HYSCMVDLL
Sbjct: 301 KRMDEGGGVEADEVTLVTVLCACSHSGLVDIGRQIFGSMIDGKFGFSPGIQHYSCMVDLL 360

Query: 361 ARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYV 420
           AR GCIEE+FELIK+MPFDATKAMWGSLLAG+RA GSLE+SEFAA+KLVEMEP NGAYY 
Sbjct: 361 ARSGCIEESFELIKNMPFDATKAMWGSLLAGSRAQGSLEISEFAARKLVEMEPENGAYYA 420

Query: 421 VLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           VLSNICAEMG+W+EVEKVREIMK  GLKKDLGSSSVE
Sbjct: 421 VLSNICAEMGKWNEVEKVREIMKVEGLKKDLGSSSVE 457

BLAST of Lag0002531 vs. NCBI nr
Match: XP_022953836.1 (pentatricopeptide repeat-containing protein At5g56310-like [Cucurbita moschata])

HSP 1 Score: 814.7 bits (2103), Expect = 4.1e-232
Identity = 399/457 (87.31%), Postives = 426/457 (93.22%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           M  F  Q  A+SN+ILSLF  SLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCH L+LL
Sbjct: 1   MAAFGVQMWAESNRILSLFATSLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHDLRLL 60

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSAL FFT  PKPHVF+CNSLIRAFSHSKIP TPLSIYAHMNRNSILPNNYTFPFLLKSL
Sbjct: 61  DSALRFFTQIPKPHVFVCNSLIRAFSHSKIPRTPLSIYAHMNRNSILPNNYTFPFLLKSL 120

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           ADF  LVSGQSVH HV++LGYVSDVYVQNS+MDVYASCGRMGLCKKVFDEMP RDVVSWT
Sbjct: 121 ADFNHLVSGQSVHAHVVKLGYVSDVYVQNSLMDVYASCGRMGLCKKVFDEMPLRDVVSWT 180

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           VLIMGYR ALMFDDALIAFEQMQYAGV PNRVTMVNALAACASFGA+EMGVWIHEFVKRR
Sbjct: 181 VLIMGYRVALMFDDALIAFEQMQYAGVVPNRVTMVNALAACASFGAIEMGVWIHEFVKRR 240

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
           GWE+DVILGTSLIDMYGKCGRI+EGLVVFQAMK+KNVYTWNALI GLALAKSGEEAIAWF
Sbjct: 241 GWEMDVILGTSLIDMYGKCGRIEEGLVVFQAMKDKNVYTWNALINGLALAKSGEEAIAWF 300

Query: 301 KKMDNE-GVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLL 360
           K+M+ E G+EADEVT++AVLCACSHSGLVD+GRQIF S+ DG+FGFSPGI+HYSCMVDLL
Sbjct: 301 KRMNEEGGIEADEVTLVAVLCACSHSGLVDIGRQIFGSMIDGKFGFSPGIQHYSCMVDLL 360

Query: 361 ARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYV 420
           AR G IEE+FELIK+MPFDATKAMWGSLLAG+RA GSLE+SEFAA+KLVEMEP NGAYY 
Sbjct: 361 ARSGRIEESFELIKNMPFDATKAMWGSLLAGSRAQGSLEMSEFAARKLVEMEPENGAYYA 420

Query: 421 VLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           VLSNICAEMG+W+EVEKVREIMK  GLKKDLGSSSVE
Sbjct: 421 VLSNICAEMGKWNEVEKVREIMKVEGLKKDLGSSSVE 457

BLAST of Lag0002531 vs. ExPASy Swiss-Prot
Match: Q9FMA1 (Pentatricopeptide repeat-containing protein At5g56310 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E13 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 8.6e-84
Identity = 170/465 (36.56%), Postives = 257/465 (55.27%), Query Frame = 0

Query: 28  IPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLLDSALLFFTHFPKPHVFICNSLIRAFSH 87
           + Q    +I+  L+ +      FI  C +   L  A   FTH P P+ ++ N++IRA S 
Sbjct: 31  LKQSHCYMIITGLNRDNLNVAKFIEACSNAGHLRYAYSVFTHQPCPNTYLHNTMIRALSL 90

Query: 88  SKIPHT---PLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVHTHVLRLGYVSD 147
              P+     +++Y  +      P+ +TFPF+LK      D+  G+ +H  V+  G+ S 
Sbjct: 91  LDEPNAHSIAITVYRKLWALCAKPDTFTFPFVLKIAVRVSDVWFGRQIHGQVVVFGFDSS 150

Query: 148 VYVQNSVMDVYASCGRMGLCKKVFDEMPQRDV---------------------------- 207
           V+V   ++ +Y SCG +G  +K+FDEM  +DV                            
Sbjct: 151 VHVVTGLIQMYFSCGGLGDARKMFDEMLVKDVNVWNALLAGYGKVGEMDEARSLLEMMPC 210

Query: 208 -----VSWTVLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGV 267
                VSWT +I GY  +    +A+  F++M    VEP+ VT++  L+ACA  G++E+G 
Sbjct: 211 WVRNEVSWTCVISGYAKSGRASEAIEVFQRMLMENVEPDEVTLLAVLSACADLGSLELGE 270

Query: 268 WIHEFVKRRGWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAK 327
            I  +V  RG    V L  ++IDMY K G I + L VF+ + E+NV TW  +I GLA   
Sbjct: 271 RICSYVDHRGMNRAVSLNNAVIDMYAKSGNITKALDVFECVNERNVVTWTTIIAGLATHG 330

Query: 328 SGEEAIAWFKKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKH 387
            G EA+A F +M   GV  ++VT +A+L ACSH G VD+G+++F S+   ++G  P I+H
Sbjct: 331 HGAEALAMFNRMVKAGVRPNDVTFIAILSACSHVGWVDLGKRLFNSM-RSKYGIHPNIEH 390

Query: 388 YSCMVDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEME 447
           Y CM+DLL R G + EA E+IK MPF A  A+WGSLLA +  H  LE+ E A  +L+++E
Sbjct: 391 YGCMIDLLGRAGKLREADEVIKSMPFKANAAIWGSLLAASNVHHDLELGERALSELIKLE 450

Query: 448 PMNGAYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           P N   Y++L+N+ + +GRW E   +R +MK  G+KK  G SS+E
Sbjct: 451 PNNSGNYMLLANLYSNLGRWDESRMMRNMMKGIGVKKMAGESSIE 494

BLAST of Lag0002531 vs. ExPASy Swiss-Prot
Match: O80488 (Pentatricopeptide repeat-containing protein At1g09190 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E70 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 2.6e-80
Identity = 173/475 (36.42%), Postives = 262/475 (55.16%), Query Frame = 0

Query: 14  KILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLLDSALLFFTHFPKP 73
           K+L L     +   +P+I A L+   LH +  +  HFI+ C SL   D A   F+H   P
Sbjct: 6   KLLRLLHGHNTRTRLPEIHAHLLRHFLHGSNLLLAHFISICGSLSNSDYANRVFSHIQNP 65

Query: 74  HVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVH 133
           +V + N++I+ +S    P   LS ++ M    I  + YT+  LLKS +   DL  G+ VH
Sbjct: 66  NVLVFNAMIKCYSLVGPPLESLSFFSSMKSRGIWADEYTYAPLLKSCSSLSDLRFGKCVH 125

Query: 134 THVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWTVLIMGY------- 193
             ++R G+     ++  V+++Y S GRMG  +KVFDEM +R+VV W ++I G+       
Sbjct: 126 GELIRTGFHRLGKIRIGVVELYTSGGRMGDAQKVFDEMSERNVVVWNLMIRGFCDSGDVE 185

Query: 194 -----------RCALMFD-------------DALIAFEQMQYAGVEPNRVTMVNALAACA 253
                      R  + ++             +AL  F +M   G +P+  T+V  L   A
Sbjct: 186 RGLHLFKQMSERSIVSWNSMISSLSKCGRDREALELFCEMIDQGFDPDEATVVTVLPISA 245

Query: 254 SFGAVEMGVWIHEFVKRRGWEVDVI-LGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWN 313
           S G ++ G WIH   +  G   D I +G +L+D Y K G ++    +F+ M+ +NV +WN
Sbjct: 246 SLGVLDTGKWIHSTAESSGLFKDFITVGNALVDFYCKSGDLEAATAIFRKMQRRNVVSWN 305

Query: 314 ALIKGLALAKSGEEAIAWFKKMDNEG-VEADEVTVLAVLCACSHSGLVDMGRQIFRSLFD 373
            LI G A+   GE  I  F  M  EG V  +E T L VL  CS++G V+ G ++F  + +
Sbjct: 306 TLISGSAVNGKGEFGIDLFDAMIEEGKVAPNEATFLGVLACCSYTGQVERGEELFGLMME 365

Query: 374 GRFGFSPGIKHYSCMVDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVS 433
            RF      +HY  MVDL++R G I EAF+ +K+MP +A  AMWGSLL+  R+HG ++++
Sbjct: 366 -RFKLEARTEHYGAMVDLMSRSGRITEAFKFLKNMPVNANAAMWGSLLSACRSHGDVKLA 425

Query: 434 EFAAKKLVEMEPMNGAYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSV 456
           E AA +LV++EP N   YV+LSN+ AE GRW +VEKVR +MK+  L+K  G S++
Sbjct: 426 EVAAMELVKIEPGNSGNYVLLSNLYAEEGRWQDVEKVRTLMKKNRLRKSTGQSTI 479

BLAST of Lag0002531 vs. ExPASy Swiss-Prot
Match: Q9SJZ3 (Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E28 PE=2 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 5.8e-80
Identity = 160/461 (34.71%), Postives = 260/461 (56.40%), Query Frame = 0

Query: 27  HIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLLDSALLFFTHFPKPHVFICNSLIRAFS 86
           H+ +++ +L V ++H N +I  H   +C     +++A   F   P   +   N LI  + 
Sbjct: 181 HVLKLRLEL-VSHVH-NASI--HMFASCGD---MENARKVFDESPVRDLVSWNCLINGYK 240

Query: 87  HSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVHTHVLRLGYVSDVY 146
                   + +Y  M    + P++ T   L+ S +  GDL  G+  + +V   G    + 
Sbjct: 241 KIGEAEKAIYVYKLMESEGVKPDDVTMIGLVSSCSMLGDLNRGKEFYEYVKENGLRMTIP 300

Query: 147 VQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWTVLIMGY-RCAL------MFD------ 206
           + N++MD+++ CG +   +++FD + +R +VSWT +I GY RC L      +FD      
Sbjct: 301 LVNALMDMFSKCGDIHEARRIFDNLEKRTIVSWTTMISGYARCGLLDVSRKLFDDMEEKD 360

Query: 207 ------------------DALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHE 266
                             DAL  F++MQ +  +P+ +TM++ L+AC+  GA+++G+WIH 
Sbjct: 361 VVLWNAMIGGSVQAKRGQDALALFQEMQTSNTKPDEITMIHCLSACSQLGALDVGIWIHR 420

Query: 267 FVKRRGWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEE 326
           ++++    ++V LGTSL+DMY KCG I E L VF  ++ +N  T+ A+I GLAL      
Sbjct: 421 YIEKYSLSLNVALGTSLVDMYAKCGNISEALSVFHGIQTRNSLTYTAIIGGLALHGDAST 480

Query: 327 AIAWFKKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCM 386
           AI++F +M + G+  DE+T + +L AC H G++  GR  F S    RF  +P +KHYS M
Sbjct: 481 AISYFNEMIDAGIAPDEITFIGLLSACCHGGMIQTGRDYF-SQMKSRFNLNPQLKHYSIM 540

Query: 387 VDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNG 446
           VDLL R G +EEA  L++ MP +A  A+WG+LL G R HG++E+ E AAKKL+E++P + 
Sbjct: 541 VDLLGRAGLLEEADRLMESMPMEADAAVWGALLFGCRMHGNVELGEKAAKKLLELDPSDS 600

Query: 447 AYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
             YV+L  +  E   W + ++ R +M ERG++K  G SS+E
Sbjct: 601 GIYVLLDGMYGEANMWEDAKRARRMMNERGVEKIPGCSSIE 633

BLAST of Lag0002531 vs. ExPASy Swiss-Prot
Match: Q9FJY7 (Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H61 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.3e-79
Identity = 165/462 (35.71%), Postives = 250/462 (54.11%), Query Frame = 0

Query: 28  IPQIQAQLIVQNLHSNPTIAHHFINTC---HSLQLLDSALLFFTHFPKPHVFICNSLIRA 87
           + QI A+++   L  +      F++ C    S   L  A + F  F +P  F+ N +IR 
Sbjct: 30  LKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYAQIVFDGFDRPDTFLWNLMIRG 89

Query: 88  FSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVHTHVLRLGYVSD 147
           FS S  P   L +Y  M  +S   N YTFP LLK+ ++         +H  + +LGY +D
Sbjct: 90  FSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAFEETTQIHAQITKLGYEND 149

Query: 148 VYVQNSVMDVYASCGRMGLCKKVFDEMPQRD----------------------------- 207
           VY  NS+++ YA  G   L   +FD +P+ D                             
Sbjct: 150 VYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIKGYVKAGKMDIALTLFRKMAE 209

Query: 208 --VVSWTVLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWI 267
              +SWT +I GY  A M  +AL  F +MQ + VEP+ V++ NAL+ACA  GA+E G WI
Sbjct: 210 KNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVSLANALSACAQLGALEQGKWI 269

Query: 268 HEFVKRRGWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSG 327
           H ++ +    +D +LG  LIDMY KCG ++E L VF+ +K+K+V  W ALI G A    G
Sbjct: 270 HSYLNKTRIRMDSVLGCVLIDMYAKCGEMEEALEVFKNIKKKSVQAWTALISGYAYHGHG 329

Query: 328 EEAIAWFKKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYS 387
            EAI+ F +M   G++ + +T  AVL ACS++GLV+ G+ IF S+ +  +   P I+HY 
Sbjct: 330 REAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKLIFYSM-ERDYNLKPTIEHYG 389

Query: 388 CMVDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPM 447
           C+VDLL R G ++EA   I++MP      +WG+LL   R H ++E+ E   + L+ ++P 
Sbjct: 390 CIVDLLGRAGLLDEAKRFIQEMPLKPNAVIWGALLKACRIHKNIELGEEIGEILIAIDPY 449

Query: 448 NGAYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSV 456
           +G  YV  +NI A   +W +  + R +MKE+G+ K  G S++
Sbjct: 450 HGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCSTI 490

BLAST of Lag0002531 vs. ExPASy Swiss-Prot
Match: Q9FIF7 (Putative pentatricopeptide repeat-containing protein At5g59200, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-E41 PE=3 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 6.4e-79
Identity = 165/473 (34.88%), Postives = 263/473 (55.60%), Query Frame = 0

Query: 15  ILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLLDSALLFFTHFPKPH 74
           ++S+  +  +  H+P I A++I      +  +    I  C +L  +D A   F++   P+
Sbjct: 32  LISVLRSCKNIAHVPSIHAKIIRTFHDQDAFVVFELIRVCSTLDSVDYAYDVFSYVSNPN 91

Query: 75  VFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVHT 134
           V++  ++I  F  S      +S+Y  M  NS+LP+NY    +LK+     DL   + +H 
Sbjct: 92  VYLYTAMIDGFVSSGRSADGVSLYHRMIHNSVLPDNYVITSVLKAC----DLKVCREIHA 151

Query: 135 HVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWTVLIMGY-RCAL--- 194
            VL+LG+ S   V   +M++Y   G +   KK+FDEMP RD V+ TV+I  Y  C     
Sbjct: 152 QVLKLGFGSSRSVGLKMMEIYGKSGELVNAKKMFDEMPDRDHVAATVMINCYSECGFIKE 211

Query: 195 ---MFDD------------------------ALIAFEQMQYAGVEPNRVTMVNALAACAS 254
              +F D                        AL  F +MQ   V  N  T V  L+AC+ 
Sbjct: 212 ALELFQDVKIKDTVCWTAMIDGLVRNKEMNKALELFREMQMENVSANEFTAVCVLSACSD 271

Query: 255 FGAVEMGVWIHEFVKRRGWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNAL 314
            GA+E+G W+H FV+ +  E+   +G +LI+MY +CG I E   VF+ M++K+V ++N +
Sbjct: 272 LGALELGRWVHSFVENQRMELSNFVGNALINMYSRCGDINEARRVFRVMRDKDVISYNTM 331

Query: 315 IKGLALAKSGEEAIAWFKKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRF 374
           I GLA+  +  EAI  F+ M N G   ++VT++A+L ACSH GL+D+G ++F S+    F
Sbjct: 332 ISGLAMHGASVEAINEFRDMVNRGFRPNQVTLVALLNACSHGGLLDIGLEVFNSM-KRVF 391

Query: 375 GFSPGIKHYSCMVDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFA 434
              P I+HY C+VDLL R G +EEA+  I+++P +    M G+LL+  + HG++E+ E  
Sbjct: 392 NVEPQIEHYGCIVDLLGRVGRLEEAYRFIENIPIEPDHIMLGTLLSACKIHGNMELGEKI 451

Query: 435 AKKLVEMEPMNGAYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           AK+L E E  +   YV+LSN+ A  G+W E  ++RE M++ G++K+ G S++E
Sbjct: 452 AKRLFESENPDSGTYVLLSNLYASSGKWKESTEIRESMRDSGIEKEPGCSTIE 499

BLAST of Lag0002531 vs. ExPASy TrEMBL
Match: A0A6J1JWL5 (pentatricopeptide repeat-containing protein At5g56310-like OS=Cucurbita maxima OX=3661 GN=LOC111488140 PE=4 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.0e-233
Identity = 400/457 (87.53%), Postives = 425/457 (93.00%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           M  F  Q  A+SN+ILSLF  SLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCH L+LL
Sbjct: 1   MAAFGVQMWAESNRILSLFATSLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHHLRLL 60

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSAL FFT  PKPHVF+CNSLIRAFSHSKIPHTPLSIYAHMNR SILPNNYTFPFLLKSL
Sbjct: 61  DSALRFFTQIPKPHVFVCNSLIRAFSHSKIPHTPLSIYAHMNRTSILPNNYTFPFLLKSL 120

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           ADF  LVSGQSVH HV++LGYVSDVYVQNS+MDVYASCGRMGLCKKVFDEMP RDVVSWT
Sbjct: 121 ADFNHLVSGQSVHAHVVKLGYVSDVYVQNSLMDVYASCGRMGLCKKVFDEMPLRDVVSWT 180

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           VLIMGYR ALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGA+EMGVWIH FVKRR
Sbjct: 181 VLIMGYRVALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAIEMGVWIHAFVKRR 240

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
           GWE+DVILGTSLIDMYGKCGRI+EGLVVFQAMK+KNVYTWNALI GLALAKSGEEAIAWF
Sbjct: 241 GWEMDVILGTSLIDMYGKCGRIEEGLVVFQAMKDKNVYTWNALINGLALAKSGEEAIAWF 300

Query: 301 KKMD-NEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLL 360
           K+MD   GVEADEVT++ VLCACSHSGLVD+GRQIF S+ DG+FGFSPGI+HYSCMVDLL
Sbjct: 301 KRMDEGGGVEADEVTLVTVLCACSHSGLVDIGRQIFGSMIDGKFGFSPGIQHYSCMVDLL 360

Query: 361 ARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYV 420
           AR GCIEE+FELIK+MPFDATKAMWGSLLAG+RA GSLE+SEFAA+KLVEMEP NGAYY 
Sbjct: 361 ARSGCIEESFELIKNMPFDATKAMWGSLLAGSRAQGSLEISEFAARKLVEMEPENGAYYA 420

Query: 421 VLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           VLSNICAEMG+W+EVEKVREIMK  GLKKDLGSSSVE
Sbjct: 421 VLSNICAEMGKWNEVEKVREIMKVEGLKKDLGSSSVE 457

BLAST of Lag0002531 vs. ExPASy TrEMBL
Match: A0A6J1GP72 (pentatricopeptide repeat-containing protein At5g56310-like OS=Cucurbita moschata OX=3662 GN=LOC111456251 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.0e-232
Identity = 399/457 (87.31%), Postives = 426/457 (93.22%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           M  F  Q  A+SN+ILSLF  SLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCH L+LL
Sbjct: 1   MAAFGVQMWAESNRILSLFATSLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHDLRLL 60

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSAL FFT  PKPHVF+CNSLIRAFSHSKIP TPLSIYAHMNRNSILPNNYTFPFLLKSL
Sbjct: 61  DSALRFFTQIPKPHVFVCNSLIRAFSHSKIPRTPLSIYAHMNRNSILPNNYTFPFLLKSL 120

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           ADF  LVSGQSVH HV++LGYVSDVYVQNS+MDVYASCGRMGLCKKVFDEMP RDVVSWT
Sbjct: 121 ADFNHLVSGQSVHAHVVKLGYVSDVYVQNSLMDVYASCGRMGLCKKVFDEMPLRDVVSWT 180

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           VLIMGYR ALMFDDALIAFEQMQYAGV PNRVTMVNALAACASFGA+EMGVWIHEFVKRR
Sbjct: 181 VLIMGYRVALMFDDALIAFEQMQYAGVVPNRVTMVNALAACASFGAIEMGVWIHEFVKRR 240

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
           GWE+DVILGTSLIDMYGKCGRI+EGLVVFQAMK+KNVYTWNALI GLALAKSGEEAIAWF
Sbjct: 241 GWEMDVILGTSLIDMYGKCGRIEEGLVVFQAMKDKNVYTWNALINGLALAKSGEEAIAWF 300

Query: 301 KKMDNE-GVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLL 360
           K+M+ E G+EADEVT++AVLCACSHSGLVD+GRQIF S+ DG+FGFSPGI+HYSCMVDLL
Sbjct: 301 KRMNEEGGIEADEVTLVAVLCACSHSGLVDIGRQIFGSMIDGKFGFSPGIQHYSCMVDLL 360

Query: 361 ARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYV 420
           AR G IEE+FELIK+MPFDATKAMWGSLLAG+RA GSLE+SEFAA+KLVEMEP NGAYY 
Sbjct: 361 ARSGRIEESFELIKNMPFDATKAMWGSLLAGSRAQGSLEMSEFAARKLVEMEPENGAYYA 420

Query: 421 VLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           VLSNICAEMG+W+EVEKVREIMK  GLKKDLGSSSVE
Sbjct: 421 VLSNICAEMGKWNEVEKVREIMKVEGLKKDLGSSSVE 457

BLAST of Lag0002531 vs. ExPASy TrEMBL
Match: A0A6J1D9C2 (pentatricopeptide repeat-containing protein At5g56310-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018217 PE=4 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.8e-232
Identity = 396/456 (86.84%), Postives = 425/456 (93.20%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           M P AAQ  AQSN+ILSL  ASLSPIHIPQIQ+QLI+QNLHS+  IAHHFIN CH L+LL
Sbjct: 3   MKPSAAQLWAQSNRILSLLAASLSPIHIPQIQSQLILQNLHSSTAIAHHFINACHFLRLL 62

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSALLFFTHFP PHVF+ NSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL
Sbjct: 63  DSALLFFTHFPNPHVFVFNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 122

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           +DF DLV GQSVHTHV++ G+VSDVYVQNS+MDVYASCGRMGLC+KVFDEMPQRDVVSWT
Sbjct: 123 SDFNDLVGGQSVHTHVVKWGFVSDVYVQNSLMDVYASCGRMGLCRKVFDEMPQRDVVSWT 182

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           VLIMGYR  LMFDDAL+AFE MQYAGVEPN VTMVNALAACA +GA+EMGVWIHEFVKRR
Sbjct: 183 VLIMGYRGGLMFDDALVAFEHMQYAGVEPNCVTMVNALAACAGYGAIEMGVWIHEFVKRR 242

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
           GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNV+TWNALIKGLALAKSGEEAIAWF
Sbjct: 243 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVFTWNALIKGLALAKSGEEAIAWF 302

Query: 301 KKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLLA 360
           K+MD EGVEADEVT++AVLCACSHSGLV+ GR+IFR+L DG +GFSPGIKH+SCMVDLLA
Sbjct: 303 KRMDEEGVEADEVTLVAVLCACSHSGLVNKGREIFRALVDGSYGFSPGIKHFSCMVDLLA 362

Query: 361 RYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYVV 420
           R GCIEEAF LIKDMPFDATKAMWGSLLAG RA+GSLEVSEFAA+KLVEMEP NGAYYVV
Sbjct: 363 RSGCIEEAFVLIKDMPFDATKAMWGSLLAGGRANGSLEVSEFAARKLVEMEPENGAYYVV 422

Query: 421 LSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           LSNI AEMGRW EVE+VR+IM+ERGLKKD GSSSVE
Sbjct: 423 LSNILAEMGRWGEVEEVRDIMRERGLKKDSGSSSVE 458

BLAST of Lag0002531 vs. ExPASy TrEMBL
Match: A0A6J1D8G0 (pentatricopeptide repeat-containing protein At1g09190-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018217 PE=4 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.8e-232
Identity = 396/456 (86.84%), Postives = 425/456 (93.20%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           M P AAQ  AQSN+ILSL  ASLSPIHIPQIQ+QLI+QNLHS+  IAHHFIN CH L+LL
Sbjct: 87  MKPSAAQLWAQSNRILSLLAASLSPIHIPQIQSQLILQNLHSSTAIAHHFINACHFLRLL 146

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSALLFFTHFP PHVF+ NSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL
Sbjct: 147 DSALLFFTHFPNPHVFVFNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 206

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           +DF DLV GQSVHTHV++ G+VSDVYVQNS+MDVYASCGRMGLC+KVFDEMPQRDVVSWT
Sbjct: 207 SDFNDLVGGQSVHTHVVKWGFVSDVYVQNSLMDVYASCGRMGLCRKVFDEMPQRDVVSWT 266

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           VLIMGYR  LMFDDAL+AFE MQYAGVEPN VTMVNALAACA +GA+EMGVWIHEFVKRR
Sbjct: 267 VLIMGYRGGLMFDDALVAFEHMQYAGVEPNCVTMVNALAACAGYGAIEMGVWIHEFVKRR 326

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
           GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNV+TWNALIKGLALAKSGEEAIAWF
Sbjct: 327 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVFTWNALIKGLALAKSGEEAIAWF 386

Query: 301 KKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLLA 360
           K+MD EGVEADEVT++AVLCACSHSGLV+ GR+IFR+L DG +GFSPGIKH+SCMVDLLA
Sbjct: 387 KRMDEEGVEADEVTLVAVLCACSHSGLVNKGREIFRALVDGSYGFSPGIKHFSCMVDLLA 446

Query: 361 RYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYVV 420
           R GCIEEAF LIKDMPFDATKAMWGSLLAG RA+GSLEVSEFAA+KLVEMEP NGAYYVV
Sbjct: 447 RSGCIEEAFVLIKDMPFDATKAMWGSLLAGGRANGSLEVSEFAARKLVEMEPENGAYYVV 506

Query: 421 LSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           LSNI AEMGRW EVE+VR+IM+ERGLKKD GSSSVE
Sbjct: 507 LSNILAEMGRWGEVEEVRDIMRERGLKKDSGSSSVE 542

BLAST of Lag0002531 vs. ExPASy TrEMBL
Match: A0A1S3BRY1 (pentatricopeptide repeat-containing protein At5g56310-like OS=Cucumis melo OX=3656 GN=LOC103492838 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.5e-227
Identity = 384/456 (84.21%), Postives = 419/456 (91.89%), Query Frame = 0

Query: 1   MTPFAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLL 60
           M PF  QF A+S +ILS+FT SLSP+HIPQIQAQLI++NLHS+P IAHHFINTCH L LL
Sbjct: 1   MRPFGTQFRAESTRILSIFTTSLSPVHIPQIQAQLILRNLHSHPLIAHHFINTCHHLHLL 60

Query: 61  DSALLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSL 120
           DSA LFFTH PKPHVFICNSLIRAF+HS IPHTPLSIY HMNRNSI PNNYTFPF+LKSL
Sbjct: 61  DSAFLFFTHIPKPHVFICNSLIRAFAHSNIPHTPLSIYTHMNRNSISPNNYTFPFVLKSL 120

Query: 121 ADFGDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWT 180
           ADF DLVSGQSVHTHV++LG+ SD+YVQN++MDVYASCG+MGLCKKVFDEM QRDVVSWT
Sbjct: 121 ADFKDLVSGQSVHTHVVKLGHDSDLYVQNTLMDVYASCGKMGLCKKVFDEMLQRDVVSWT 180

Query: 181 VLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRR 240
           +LIMGYR +LM DDALI FEQMQYAGVEPNRVT+VNALAACASFGA+EMGVWIHEFVK +
Sbjct: 181 ILIMGYRVSLMLDDALIVFEQMQYAGVEPNRVTIVNALAACASFGAIEMGVWIHEFVKTK 240

Query: 241 GWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWF 300
            WEVDV+LGT+LIDMYGKCGRIKE L VFQAMKEKNVYTWN LI GLALAKSGEEAIAWF
Sbjct: 241 RWEVDVVLGTALIDMYGKCGRIKEALAVFQAMKEKNVYTWNVLINGLALAKSGEEAIAWF 300

Query: 301 KKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLLA 360
           K+MD EGVEAD+VT++AVLCACSHSGLV+ GRQIFRSL  GRFGFSP IKHYSCMVD+LA
Sbjct: 301 KRMDEEGVEADDVTLVAVLCACSHSGLVNSGRQIFRSLIHGRFGFSPEIKHYSCMVDILA 360

Query: 361 RYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYVV 420
           R GCIEEAF +IKDMPF+ATKAMWGSLL G+RAHG+LEVSE AA+KLVEMEP NGAYYVV
Sbjct: 361 RNGCIEEAFVMIKDMPFEATKAMWGSLLTGSRAHGNLEVSEIAARKLVEMEPENGAYYVV 420

Query: 421 LSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           LSNI AEMG+WSEVEKVREIMKERGLKKDLGSSSVE
Sbjct: 421 LSNIYAEMGKWSEVEKVREIMKERGLKKDLGSSSVE 456

BLAST of Lag0002531 vs. TAIR 10
Match: AT5G56310.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 6.1e-85
Identity = 170/465 (36.56%), Postives = 257/465 (55.27%), Query Frame = 0

Query: 28  IPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLLDSALLFFTHFPKPHVFICNSLIRAFSH 87
           + Q    +I+  L+ +      FI  C +   L  A   FTH P P+ ++ N++IRA S 
Sbjct: 31  LKQSHCYMIITGLNRDNLNVAKFIEACSNAGHLRYAYSVFTHQPCPNTYLHNTMIRALSL 90

Query: 88  SKIPHT---PLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVHTHVLRLGYVSD 147
              P+     +++Y  +      P+ +TFPF+LK      D+  G+ +H  V+  G+ S 
Sbjct: 91  LDEPNAHSIAITVYRKLWALCAKPDTFTFPFVLKIAVRVSDVWFGRQIHGQVVVFGFDSS 150

Query: 148 VYVQNSVMDVYASCGRMGLCKKVFDEMPQRDV---------------------------- 207
           V+V   ++ +Y SCG +G  +K+FDEM  +DV                            
Sbjct: 151 VHVVTGLIQMYFSCGGLGDARKMFDEMLVKDVNVWNALLAGYGKVGEMDEARSLLEMMPC 210

Query: 208 -----VSWTVLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGV 267
                VSWT +I GY  +    +A+  F++M    VEP+ VT++  L+ACA  G++E+G 
Sbjct: 211 WVRNEVSWTCVISGYAKSGRASEAIEVFQRMLMENVEPDEVTLLAVLSACADLGSLELGE 270

Query: 268 WIHEFVKRRGWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAK 327
            I  +V  RG    V L  ++IDMY K G I + L VF+ + E+NV TW  +I GLA   
Sbjct: 271 RICSYVDHRGMNRAVSLNNAVIDMYAKSGNITKALDVFECVNERNVVTWTTIIAGLATHG 330

Query: 328 SGEEAIAWFKKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKH 387
            G EA+A F +M   GV  ++VT +A+L ACSH G VD+G+++F S+   ++G  P I+H
Sbjct: 331 HGAEALAMFNRMVKAGVRPNDVTFIAILSACSHVGWVDLGKRLFNSM-RSKYGIHPNIEH 390

Query: 388 YSCMVDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEME 447
           Y CM+DLL R G + EA E+IK MPF A  A+WGSLLA +  H  LE+ E A  +L+++E
Sbjct: 391 YGCMIDLLGRAGKLREADEVIKSMPFKANAAIWGSLLAASNVHHDLELGERALSELIKLE 450

Query: 448 PMNGAYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
           P N   Y++L+N+ + +GRW E   +R +MK  G+KK  G SS+E
Sbjct: 451 PNNSGNYMLLANLYSNLGRWDESRMMRNMMKGIGVKKMAGESSIE 494

BLAST of Lag0002531 vs. TAIR 10
Match: AT1G09190.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 300.8 bits (769), Expect = 1.8e-81
Identity = 173/475 (36.42%), Postives = 262/475 (55.16%), Query Frame = 0

Query: 14  KILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLLDSALLFFTHFPKP 73
           K+L L     +   +P+I A L+   LH +  +  HFI+ C SL   D A   F+H   P
Sbjct: 6   KLLRLLHGHNTRTRLPEIHAHLLRHFLHGSNLLLAHFISICGSLSNSDYANRVFSHIQNP 65

Query: 74  HVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVH 133
           +V + N++I+ +S    P   LS ++ M    I  + YT+  LLKS +   DL  G+ VH
Sbjct: 66  NVLVFNAMIKCYSLVGPPLESLSFFSSMKSRGIWADEYTYAPLLKSCSSLSDLRFGKCVH 125

Query: 134 THVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWTVLIMGY------- 193
             ++R G+     ++  V+++Y S GRMG  +KVFDEM +R+VV W ++I G+       
Sbjct: 126 GELIRTGFHRLGKIRIGVVELYTSGGRMGDAQKVFDEMSERNVVVWNLMIRGFCDSGDVE 185

Query: 194 -----------RCALMFD-------------DALIAFEQMQYAGVEPNRVTMVNALAACA 253
                      R  + ++             +AL  F +M   G +P+  T+V  L   A
Sbjct: 186 RGLHLFKQMSERSIVSWNSMISSLSKCGRDREALELFCEMIDQGFDPDEATVVTVLPISA 245

Query: 254 SFGAVEMGVWIHEFVKRRGWEVDVI-LGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWN 313
           S G ++ G WIH   +  G   D I +G +L+D Y K G ++    +F+ M+ +NV +WN
Sbjct: 246 SLGVLDTGKWIHSTAESSGLFKDFITVGNALVDFYCKSGDLEAATAIFRKMQRRNVVSWN 305

Query: 314 ALIKGLALAKSGEEAIAWFKKMDNEG-VEADEVTVLAVLCACSHSGLVDMGRQIFRSLFD 373
            LI G A+   GE  I  F  M  EG V  +E T L VL  CS++G V+ G ++F  + +
Sbjct: 306 TLISGSAVNGKGEFGIDLFDAMIEEGKVAPNEATFLGVLACCSYTGQVERGEELFGLMME 365

Query: 374 GRFGFSPGIKHYSCMVDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVS 433
            RF      +HY  MVDL++R G I EAF+ +K+MP +A  AMWGSLL+  R+HG ++++
Sbjct: 366 -RFKLEARTEHYGAMVDLMSRSGRITEAFKFLKNMPVNANAAMWGSLLSACRSHGDVKLA 425

Query: 434 EFAAKKLVEMEPMNGAYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSV 456
           E AA +LV++EP N   YV+LSN+ AE GRW +VEKVR +MK+  L+K  G S++
Sbjct: 426 EVAAMELVKIEPGNSGNYVLLSNLYAEEGRWQDVEKVRTLMKKNRLRKSTGQSTI 479

BLAST of Lag0002531 vs. TAIR 10
Match: AT2G22410.1 (SLOW GROWTH 1 )

HSP 1 Score: 299.7 bits (766), Expect = 4.1e-81
Identity = 160/461 (34.71%), Postives = 260/461 (56.40%), Query Frame = 0

Query: 27  HIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLLDSALLFFTHFPKPHVFICNSLIRAFS 86
           H+ +++ +L V ++H N +I  H   +C     +++A   F   P   +   N LI  + 
Sbjct: 181 HVLKLRLEL-VSHVH-NASI--HMFASCGD---MENARKVFDESPVRDLVSWNCLINGYK 240

Query: 87  HSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVHTHVLRLGYVSDVY 146
                   + +Y  M    + P++ T   L+ S +  GDL  G+  + +V   G    + 
Sbjct: 241 KIGEAEKAIYVYKLMESEGVKPDDVTMIGLVSSCSMLGDLNRGKEFYEYVKENGLRMTIP 300

Query: 147 VQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWTVLIMGY-RCAL------MFD------ 206
           + N++MD+++ CG +   +++FD + +R +VSWT +I GY RC L      +FD      
Sbjct: 301 LVNALMDMFSKCGDIHEARRIFDNLEKRTIVSWTTMISGYARCGLLDVSRKLFDDMEEKD 360

Query: 207 ------------------DALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHE 266
                             DAL  F++MQ +  +P+ +TM++ L+AC+  GA+++G+WIH 
Sbjct: 361 VVLWNAMIGGSVQAKRGQDALALFQEMQTSNTKPDEITMIHCLSACSQLGALDVGIWIHR 420

Query: 267 FVKRRGWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEE 326
           ++++    ++V LGTSL+DMY KCG I E L VF  ++ +N  T+ A+I GLAL      
Sbjct: 421 YIEKYSLSLNVALGTSLVDMYAKCGNISEALSVFHGIQTRNSLTYTAIIGGLALHGDAST 480

Query: 327 AIAWFKKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCM 386
           AI++F +M + G+  DE+T + +L AC H G++  GR  F S    RF  +P +KHYS M
Sbjct: 481 AISYFNEMIDAGIAPDEITFIGLLSACCHGGMIQTGRDYF-SQMKSRFNLNPQLKHYSIM 540

Query: 387 VDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNG 446
           VDLL R G +EEA  L++ MP +A  A+WG+LL G R HG++E+ E AAKKL+E++P + 
Sbjct: 541 VDLLGRAGLLEEADRLMESMPMEADAAVWGALLFGCRMHGNVELGEKAAKKLLELDPSDS 600

Query: 447 AYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSVE 457
             YV+L  +  E   W + ++ R +M ERG++K  G SS+E
Sbjct: 601 GIYVLLDGMYGEANMWEDAKRARRMMNERGVEKIPGCSSIE 633

BLAST of Lag0002531 vs. TAIR 10
Match: AT5G66520.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 9.2e-81
Identity = 165/462 (35.71%), Postives = 250/462 (54.11%), Query Frame = 0

Query: 28  IPQIQAQLIVQNLHSNPTIAHHFINTC---HSLQLLDSALLFFTHFPKPHVFICNSLIRA 87
           + QI A+++   L  +      F++ C    S   L  A + F  F +P  F+ N +IR 
Sbjct: 30  LKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYAQIVFDGFDRPDTFLWNLMIRG 89

Query: 88  FSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSLADFGDLVSGQSVHTHVLRLGYVSD 147
           FS S  P   L +Y  M  +S   N YTFP LLK+ ++         +H  + +LGY +D
Sbjct: 90  FSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAFEETTQIHAQITKLGYEND 149

Query: 148 VYVQNSVMDVYASCGRMGLCKKVFDEMPQRD----------------------------- 207
           VY  NS+++ YA  G   L   +FD +P+ D                             
Sbjct: 150 VYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIKGYVKAGKMDIALTLFRKMAE 209

Query: 208 --VVSWTVLIMGYRCALMFDDALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWI 267
              +SWT +I GY  A M  +AL  F +MQ + VEP+ V++ NAL+ACA  GA+E G WI
Sbjct: 210 KNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVSLANALSACAQLGALEQGKWI 269

Query: 268 HEFVKRRGWEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSG 327
           H ++ +    +D +LG  LIDMY KCG ++E L VF+ +K+K+V  W ALI G A    G
Sbjct: 270 HSYLNKTRIRMDSVLGCVLIDMYAKCGEMEEALEVFKNIKKKSVQAWTALISGYAYHGHG 329

Query: 328 EEAIAWFKKMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYS 387
            EAI+ F +M   G++ + +T  AVL ACS++GLV+ G+ IF S+ +  +   P I+HY 
Sbjct: 330 REAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKLIFYSM-ERDYNLKPTIEHYG 389

Query: 388 CMVDLLARYGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPM 447
           C+VDLL R G ++EA   I++MP      +WG+LL   R H ++E+ E   + L+ ++P 
Sbjct: 390 CIVDLLGRAGLLDEAKRFIQEMPLKPNAVIWGALLKACRIHKNIELGEEIGEILIAIDPY 449

Query: 448 NGAYYVVLSNICAEMGRWSEVEKVREIMKERGLKKDLGSSSV 456
           +G  YV  +NI A   +W +  + R +MKE+G+ K  G S++
Sbjct: 450 HGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCSTI 490

BLAST of Lag0002531 vs. TAIR 10
Match: AT1G06150.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 4.5e-80
Identity = 163/515 (31.65%), Postives = 273/515 (53.01%), Query Frame = 0

Query: 4    FAAQFCAQSNKILSLFTASLSPIHIPQIQAQLIVQNLHSNPTIAHHFINTCHSLQLLDSA 63
            F+A        +  +     +P  +    A +I  +L+ +  + + FI  C S + LD A
Sbjct: 765  FSASLSLAPPNLKKIIKQCSTPKLLESALAAMIKTSLNQDCRLMNQFITACTSFKRLDLA 824

Query: 64   LLFFTHFPKPHVFICNSLIRAFSHSKIPHTPLSIYAHMNRNSILPNNYTFPFLLKSLADF 123
            +   T   +P+VF+ N+L + F     P   L +Y  M R+S+ P++YT+  L+K+ + F
Sbjct: 825  VSTMTQMQEPNVFVYNALFKGFVTCSHPIRSLELYVRMLRDSVSPSSYTYSSLVKA-SSF 884

Query: 124  GDLVSGQSVHTHVLRLGYVSDVYVQNSVMDVYASCGRMGLCKKVFDEMPQRDVVSWTVLI 183
                 G+S+  H+ + G+   V +Q +++D Y++ GR+   +KVFDEMP+RD ++WT ++
Sbjct: 885  ASRF-GESLQAHIWKFGFGFHVKIQTTLIDFYSATGRIREARKVFDEMPERDDIAWTTMV 944

Query: 184  MGYRCALMFD-------------------------------------------------- 243
              YR  L  D                                                  
Sbjct: 945  SAYRRVLDMDSANSLANQMSEKNEATSNCLINGYMGLGNLEQAESLFNQMPVKDIISWTT 1004

Query: 244  ------------DALIAFEQMQYAGVEPNRVTMVNALAACASFGAVEMGVWIHEFVKRRG 303
                        +A+  F +M   G+ P+ VTM   ++ACA  G +E+G  +H +  + G
Sbjct: 1005 MIKGYSQNKRYREAIAVFYKMMEEGIIPDEVTMSTVISACAHLGVLEIGKEVHMYTLQNG 1064

Query: 304  WEVDVILGTSLIDMYGKCGRIKEGLVVFQAMKEKNVYTWNALIKGLALAKSGEEAIAWFK 363
            + +DV +G++L+DMY KCG ++  L+VF  + +KN++ WN++I+GLA     +EA+  F 
Sbjct: 1065 FVLDVYIGSALVDMYSKCGSLERALLVFFNLPKKNLFCWNSIIEGLAAHGFAQEALKMFA 1124

Query: 364  KMDNEGVEADEVTVLAVLCACSHSGLVDMGRQIFRSLFDGRFGFSPGIKHYSCMVDLLAR 423
            KM+ E V+ + VT ++V  AC+H+GLVD GR+I+RS+ D  +     ++HY  MV L ++
Sbjct: 1125 KMEMESVKPNAVTFVSVFTACTHAGLVDEGRRIYRSMIDD-YSIVSNVEHYGGMVHLFSK 1184

Query: 424  YGCIEEAFELIKDMPFDATKAMWGSLLAGARAHGSLEVSEFAAKKLVEMEPMNGAYYVVL 456
             G I EA ELI +M F+    +WG+LL G R H +L ++E A  KL+ +EPMN  YY +L
Sbjct: 1185 AGLIYEALELIGNMEFEPNAVIWGALLDGCRIHKNLVIAEIAFNKLMVLEPMNSGYYFLL 1244

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898992.19.7e-24290.13pentatricopeptide repeat-containing protein At5g56310-like [Benincasa hispida][more]
XP_023548659.13.0e-23587.75pentatricopeptide repeat-containing protein At5g56310-like [Cucurbita pepo subsp... [more]
KAG6575842.12.6e-23487.75Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_022991568.12.2e-23387.53pentatricopeptide repeat-containing protein At5g56310-like [Cucurbita maxima][more]
XP_022953836.14.1e-23287.31pentatricopeptide repeat-containing protein At5g56310-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FMA18.6e-8436.56Pentatricopeptide repeat-containing protein At5g56310 OS=Arabidopsis thaliana OX... [more]
O804882.6e-8036.42Pentatricopeptide repeat-containing protein At1g09190 OS=Arabidopsis thaliana OX... [more]
Q9SJZ35.8e-8034.71Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidop... [more]
Q9FJY71.3e-7935.71Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX... [more]
Q9FIF76.4e-7934.88Putative pentatricopeptide repeat-containing protein At5g59200, chloroplastic OS... [more]
Match NameE-valueIdentityDescription
A0A6J1JWL51.0e-23387.53pentatricopeptide repeat-containing protein At5g56310-like OS=Cucurbita maxima O... [more]
A0A6J1GP722.0e-23287.31pentatricopeptide repeat-containing protein At5g56310-like OS=Cucurbita moschata... [more]
A0A6J1D9C29.8e-23286.84pentatricopeptide repeat-containing protein At5g56310-like isoform X2 OS=Momordi... [more]
A0A6J1D8G09.8e-23286.84pentatricopeptide repeat-containing protein At1g09190-like isoform X1 OS=Momordi... [more]
A0A1S3BRY12.5e-22784.21pentatricopeptide repeat-containing protein At5g56310-like OS=Cucumis melo OX=36... [more]
Match NameE-valueIdentityDescription
AT5G56310.16.1e-8536.56Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G09190.11.8e-8136.42Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G22410.14.1e-8134.71SLOW GROWTH 1 [more]
AT5G66520.19.2e-8135.71Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G06150.14.5e-8031.65basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 275..322
e-value: 1.7E-8
score: 34.5
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 147..176
e-value: 4.8E-4
score: 18.1
coord: 352..375
e-value: 0.0011
score: 17.0
coord: 278..312
e-value: 8.5E-7
score: 26.8
coord: 250..277
e-value: 3.6E-4
score: 18.5
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 351..375
e-value: 0.0017
score: 18.5
coord: 149..175
e-value: 0.0013
score: 18.8
coord: 177..207
e-value: 0.0015
score: 18.7
coord: 418..446
e-value: 0.25
score: 11.7
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 276..310
score: 10.709248
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 414..448
score: 8.516988
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 175..209
score: 9.700809
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 245..275
score: 8.593717
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 31..136
e-value: 5.7E-9
score: 37.5
coord: 137..227
e-value: 5.6E-20
score: 73.4
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 228..343
e-value: 2.8E-26
score: 94.7
NoneNo IPR availablePANTHERPTHR47924PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 6..456
NoneNo IPR availablePANTHERPTHR47924:SF31SUBFAMILY NOT NAMEDcoord: 6..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002531.1Lag0002531.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0009451 RNA modification
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0005515 protein binding
molecular_function GO:0003723 RNA binding