Lag0002524 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002524
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	wee1-like protein kinase
Location	chr4: 43591745 .. 43597055 (-)
RNA-Seq Expression	Lag0002524
Synteny	Lag0002524

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGAGGAAATACAAAACCCTAAGAACGACGATGAGGAAGAAGATGAAAGGGGCATTAACGAGGCACTTGCAGGTTCATCTAGGCCAGGTTTCTCTCGCCCCTCTCCACCTCAACCGCGCCTCTTCCGCCGCCTCTTGCTTCCAGGACTTGCCTGAATCTGAAGCTCCCGCCACGGTCAACGGTTCCGATGCTCTCCCCTCCTCGTCTCCTAACTTCGAGGACAGAGACTTCATTCTTAGCCAAGACTTCTTTTGGTGAGATTTGTATCGGTTTTTGCTTTTCCAGTTGGTTTGAGCTGCTGTTGATGGATGGGGAGTTGTTGATTGTTGTTTGTTGTCTTTCTAGAGGTTGATAGTGTTACGAGGTGGAAGTTAGTTAATTCCTGTTCGAAGTAGTTCGATAGACGGGACAGTGTAGTGTGTGGTTCACTAATCATTGAATACTTCTAGGAACTACGTTGCCTTTTACTGAGTTTTCTATCACTTTCGCTTATTTGAGTTAGTTCTTTTGAGTTCCTGTTGATGGATGTAGGGAGTAGTTGATTATTTGTTGTTATTTCACCATGGTTTAAGTTGCTTATTAGGTACGGAATGGAAGTCGTTTCCTATGAAAGTAACTTAGGTAGACAGAGTAAAGTAGTATGATTTACTAATTATTGAATACATTTTGGAAATTACTTTGCTTGTTACTATTGAGATTGGTATACATGGATACCAAAGGGTATCTGGTGAGATTGAAGGCTGCATTGTAACTGGCCAGATCATTTTTGGTGTTTAACCTTGGGTTTTTGTTTTTTCTTTGCAGCACTCCTGACTATATCACCCCGGACAATCAAAATCTGTTGAATGGTTTGGATTGCAACGAGGTGAGAGCAGTTAATGGAGAAAGCTAACCAGGCTAAAAACCAGGGTTTTTCAGAAAAAAATGGCTAATTCTCTAATTTTTCTGGGTGTCCTTGTCTTGGCAGGAAACCACTCCTTGCCCCAAATCTCCAGAGAAGATCACTACTATTAAAACGAAGAGGAAAATACATGGTAAGTTTATATTAGTGGTGATCTTGAATTTTTATCTGAGTTAGTTAACTGTATTGAGTGGGACTCAGTGTTTTAAAAGGCTCAAGGTGCACTAAGGCGCAATGGCCTCCTGGGGCCTAGGTGCAAGGCGCACAAAAAGGCATGAGCTTTTTTCTTTAAGGCGCACTATATAGAAAAAAAACTTACAAAAATATATACATGTATATAAAACACTTTCATGACAAGTAATGAAATTTTTAACCAAGAAAAATAGATAAAAGATAAATAAGGTTCAAATATTAGTCTTCCAACATAGAGTCAAATGTCAATACTCCACAAAAAAAATAGTGAGTTTAGTATTGTTAATATGCCTTCTGACAAGTATACATGTATATAAAACACTTTCATGACAAGTAATGAAATTTTTAACCAAGAAAAATAGATAAAAGATAAATAAGGTTCAAATATTAGTCTTCCAACATAGAGTCAAATGTCAATACTCCACAAAAAAAATAGTGAGTTTAGTATTGTTAATATGCCTTCTGACATTCTGAATGACCAAGTATGATGATGTCAATACTCCACAAAAAATATATGCCTTGAATATGATATTTGACACTCCGAATGACCAAGTACGGGGATCTCGATTCATAGCAAGTATTTAGGTTTAAAAAAAATTAAAACATTTATAAAAATGTGAAGCCCCATAGGCACCTAAGGCGCGTGCCTTGGCCACGCCTTCCTCACTGTGCCTTGCTTCGAGCGAGGCACTAAGGTTGCGCCTTGCACCTAGGCGCGCTCGAGCGCACGCCTTTTAAAACACTGGTGGGACTGATATGAGGATCAGAAGATCACATGCATTAATTTACTGCCATATACTCAATTATAATTGTGTTGAACCTGAACTTTCCAAACCAATAATGTACAATGTACATGGTGTTAAGAGAGCTCATTAAGTTGTTATTACTAATTTCTGTTATAAATGATTGTAAACATTTTCTGCTGTAAACAGTTTTAACCATATTTATTTTGCATTCACAGCACCCTATATATAAGGGTTACTGATATTTGATCACATTTAATGAGAAATACAAATCCTTTTGTGGTCATATCAATTTCTCATTCTCTCAATATCTTGAAACATGGTTAATCACTTGCAACAGATAATGACCAGTGTCCTAGTCATTTATCAATGTGGAATTTAAGACTGATCTGAAATTATTTTGGGGCTGACAACCTCATGTTCCTTTTCAATATCAGTTATATAAATTGTTGAATGGTGTAATATAATCACTTTCTTTGTTGAATATTCATTTTGTCACAGATGGGCTTTTAGTGCATCCTCTGAGTCCAAGTTTGTCTGGCCGTGAACAAGTTGTGGAGCTTGGAAATGACACTTTTGGGACAGATGATGTGGATATGGAGAAAGAGGCAATGGCAATGCCGAAGAAACCAAACTATGTTTCTCAATCAGCAGTTGCTCTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAACCCATACTTGAAAGATGCTTCTGACGTAGGTGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGTACATTTCATTTAGTTCTTAAAGACATCAAAAGTAATAAGACATTTTTGAAAGCATCTTTTGTAATGACAATGCATTTATGTTAAATGAATTAGTTTCTTGGTTTTCAATTTTGTGTAGGATTTTTTCCTGCGCTGTTTAGTGGAGATGGTCTTTCAAGATATCACACTGATTTTCATGAGATAAAGGTATTCAATCCAGAAACAAGCCTCTGCAACAAATAGAGATTGGATTTTTTTCCCTTCATATTTCAATTCAAGCTGTCAAATTTTGTAATAACTTCAATCTATTCAGCAAATAGGAACTGGAAATTTTAGCCGTGTCTTCAAGGTATTTAAGAGAATTGATGGTTGCTTGTATGCTGTCAAACAAAGCACAAGACCATTGCATCAAGACGTAGAGAGGTTACTTCTTTTGTTGTGTCTCTATTTGTTTAACCTCTAAATAGTAGTGAATGTGTATTTTAAGTTTTTTCTTCCCTTTGCAGGAGGCGAGCTTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGTTAGCAAATTTATTTAGATATGTTTTTCTGTTCTATTGTATTTTTATTTCTATTGTCATTATTTTTTTTCTCTAGAGCAAATTTTATCTTTTCTTGACTCTTGTCCTCTGTTGAACGTGTAGGATCTCATGAAAACATCGTTGGTTACTACTCTTCGTGGTTTGAAAATGAGCAACTCTACATTCAAATGGAACTCTGCGATTGTAGCTTATCCATTGGCCGGTATTCTCACCCATTCTCTGAGGCAGATGCGCTGAGAGCCTTGTTTCAGGTGAGCTGTAGTCATTTTCTGCTTGTGAAACATCATTGCCAAAAATATTTCTTTTGTTAACATATGAAAGAAAGCTTAATTTTGATTTGGAAGAGTAAACTAGATGTTTCTGCACTTCATTCAATACGTTGCTTTAAAATAATCATCTGGAATTTTGTTATGAATCATGTATTGATGTTTTCTGGTTATCCATTCCAATTCTTATGTAGCTTTAATATTTAATATAAGTTTTATCCCTTCCAATTTTAAAGTTCTAGAACTAGGAACTTTTGAAATTTCATGGCCTGTCTGCTGATAATGAAACTACAAACAGATTGCAAAAGCATTGCAGTTTGTGCACGAGAAAGGAGTAGCTCATTTAGATGTGAAACCTGACAATATTTATGTCAAAGATGGTGTTTATAAACTTGGTGATTTTGGATGCGCTACTCTTCTTGATAAAAGCCTACCAATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAGAGATATGATTATCTTGACAAGGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAGATTGTGCGAGGTTCAACATTGCCACAAGCACATTTCATGAATCTGAAGGAGGGAAAATTGCCGCTCCTTCCTGGTCACTCGTTGCAATTTCAAAACTTAATCAAGGTACTTAATACTTGTCTAATATTTTGTTTTCTTTTCATTTCCATCAACTATACCTTTTGGGTCTGATCTTCTTTGACTCAGCCTTTGTGGACTAACCTTGTGGAGGAATCCTTTTTTGGGCCTAATCTTAATCTTTGACTCGCCCTATGTGGACAAACCTTGTGTTGGAATTCTTAGATTTTTGGGGGCATTGTGATTCTTACCAATGTTTGCATTTCTTCCCATGAGTTTTACTCTACTTTAAAAATGTAAATAGTTATTGTGTTTATGCTACCAAAAGTATACTTGAAAGAAAGTTACATCCCTACCTTCCAAAAGATAAAAAATGCTGCGGTTATTTTTTCATTATGCAGTTGTCTCCAAATTATGTTAACCCATGATGTATGTATTTGGATGACAATTGTTTTCTTTTTTCTTTTTTGTTAGATTCCTTATTCTGTAAACAGGAGTCAATATTTTCCAGATGGAATATTTGATTTTGTTTGTTTGAACCGTCTGAACACAAAATGCTATATTGTGTTTTTTTTTTTTTTGGAAGATGAAATAAATAGACCAGGCATATGCTTTATCAGAATTAACTAAGTTTAGATATTCTTTTAGCTGTCTACTCCACATTTATATAGTTTGAGTAGGCTGCTTCCATTCTTTTATTGGTAGGAATATTGTTAGAGAAGCCTGATTTTAGCAGTTCAGCATAATTCATCAAACAAAAATCCATAAGATGTATTTTTCAAGTGTCTTTCAAGTCTTTTAGCGGTAAACTTGATAATTAGAAATTTTAACTAGCCTTTGAGTGGATGAAGTCGTCTAGACTCTAGAAGATTGGTGGAATGGATCATGAGCATGCGAAGAACAATTTGATTTCCCATATCCCATTATGAAGTAAAATTATAGAAGAAACTTACCTCCGATAAAATCATGGATTTTAGATTGACTTTGTATAATCATTAGGAAATGTTCACTATTTACTTTATTATCTTGGAATTTCATTAGAGATGTTCAGGACCTTGCAATTGCTTACTCTCTTTTTGATTGATTGATAACTCTAACAGGCCATGGTGGATCCCGACCCAGCACGACGGCCTTCAGCGTGGGAAATTATTGAGAATCCCATATTTGATAAGGTCAGAAATCATCCAAATTCATAAGTCAACATCAAACATCGTGTCTTGCTATTGTTTCATCTTTTCCACAGATGGGCTTTGTATGTGCAATCCCTTGGTATCAGCCTGCTGCTATTTTGTAA

mRNA sequence

ATGAGGAGGAAATACAAAACCCTAAGAACGACGATGAGGAAGAAGATGAAAGGGGCATTAACGAGGCACTTGCAGGTTCATCTAGGCCAGGTTTCTCTCGCCCCTCTCCACCTCAACCGCGCCTCTTCCGCCGCCTCTTGCTTCCAGGACTTGCCTGAATCTGAAGCTCCCGCCACGGTCAACGGTTCCGATGCTCTCCCCTCCTCGTCTCCTAACTTCGAGGACAGAGACTTCATTCTTAGCCAAGACTTCTTTTGCACTCCTGACTATATCACCCCGGACAATCAAAATCTGTTGAATGGTTTGGATTGCAACGAGGAAACCACTCCTTGCCCCAAATCTCCAGAGAAGATCACTACTATTAAAACGAAGAGGAAAATACATGATGGGCTTTTAGTGCATCCTCTGAGTCCAAGTTTGTCTGGCCGTGAACAAGTTGTGGAGCTTGGAAATGACACTTTTGGGACAGATGATGTGGATATGGAGAAAGAGGCAATGGCAATGCCGAAGAAACCAAACTATGTTTCTCAATCAGCAGTTGCTCTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAACCCATACTTGAAAGATGCTTCTGACGTAGGTGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGATTTTTTCCTGCGCTGTTTAGTGGAGATGGTCTTTCAAGATATCACACTGATTTTCATGAGATAAAGCAAATAGGAACTGGAAATTTTAGCCGTGTCTTCAAGGTATTTAAGAGAATTGATGGTTGCTTGTATGCTGTCAAACAAAGCACAAGACCATTGCATCAAGACGTAGAGAGGAGGCGAGCTTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGATCTCATGAAAACATCGTTGGTTACTACTCTTCGTGGTTTGAAAATGAGCAACTCTACATTCAAATGGAACTCTGCGATTGTAGCTTATCCATTGGCCGGTATTCTCACCCATTCTCTGAGGCAGATGCGCTGAGAGCCTTGTTTCAGATTGCAAAAGCATTGCAGTTTGTGCACGAGAAAGGAGTAGCTCATTTAGATGTGAAACCTGACAATATTTATGTCAAAGATGGTGTTTATAAACTTGGTGATTTTGGATGCGCTACTCTTCTTGATAAAAGCCTACCAATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAGAGATATGATTATCTTGACAAGGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAGATTGTGCGAGGTTCAACATTGCCACAAGCACATTTCATGAATCTGAAGGAGGGAAAATTGCCGCTCCTTCCTGGTCACTCGTTGCAATTTCAAAACTTAATCAAGGCCATGGTGGATCCCGACCCAGCACGACGGCCTTCAGCGTGGGAAATTATTGAGAATCCCATATTTGATAAGATGGGCTTTGTATGTGCAATCCCTTGGTATCAGCCTGCTGCTATTTTGTAA

Coding sequence (CDS)

ATGAGGAGGAAATACAAAACCCTAAGAACGACGATGAGGAAGAAGATGAAAGGGGCATTAACGAGGCACTTGCAGGTTCATCTAGGCCAGGTTTCTCTCGCCCCTCTCCACCTCAACCGCGCCTCTTCCGCCGCCTCTTGCTTCCAGGACTTGCCTGAATCTGAAGCTCCCGCCACGGTCAACGGTTCCGATGCTCTCCCCTCCTCGTCTCCTAACTTCGAGGACAGAGACTTCATTCTTAGCCAAGACTTCTTTTGCACTCCTGACTATATCACCCCGGACAATCAAAATCTGTTGAATGGTTTGGATTGCAACGAGGAAACCACTCCTTGCCCCAAATCTCCAGAGAAGATCACTACTATTAAAACGAAGAGGAAAATACATGATGGGCTTTTAGTGCATCCTCTGAGTCCAAGTTTGTCTGGCCGTGAACAAGTTGTGGAGCTTGGAAATGACACTTTTGGGACAGATGATGTGGATATGGAGAAAGAGGCAATGGCAATGCCGAAGAAACCAAACTATGTTTCTCAATCAGCAGTTGCTCTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAACCCATACTTGAAAGATGCTTCTGACGTAGGTGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGATTTTTTCCTGCGCTGTTTAGTGGAGATGGTCTTTCAAGATATCACACTGATTTTCATGAGATAAAGCAAATAGGAACTGGAAATTTTAGCCGTGTCTTCAAGGTATTTAAGAGAATTGATGGTTGCTTGTATGCTGTCAAACAAAGCACAAGACCATTGCATCAAGACGTAGAGAGGAGGCGAGCTTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGATCTCATGAAAACATCGTTGGTTACTACTCTTCGTGGTTTGAAAATGAGCAACTCTACATTCAAATGGAACTCTGCGATTGTAGCTTATCCATTGGCCGGTATTCTCACCCATTCTCTGAGGCAGATGCGCTGAGAGCCTTGTTTCAGATTGCAAAAGCATTGCAGTTTGTGCACGAGAAAGGAGTAGCTCATTTAGATGTGAAACCTGACAATATTTATGTCAAAGATGGTGTTTATAAACTTGGTGATTTTGGATGCGCTACTCTTCTTGATAAAAGCCTACCAATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAGAGATATGATTATCTTGACAAGGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAGATTGTGCGAGGTTCAACATTGCCACAAGCACATTTCATGAATCTGAAGGAGGGAAAATTGCCGCTCCTTCCTGGTCACTCGTTGCAATTTCAAAACTTAATCAAGGCCATGGTGGATCCCGACCCAGCACGACGGCCTTCAGCGTGGGAAATTATTGAGAATCCCATATTTGATAAGATGGGCTTTGTATGTGCAATCCCTTGGTATCAGCCTGCTGCTATTTTGTAA

Protein sequence

MRRKYKTLRTTMRKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAPATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEKITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIFDKMGFVCAIPWYQPAAIL

Homology

[Close]

BLAST of Lag0002524 vs. NCBI nr
Match: XP_038876752.1 (wee1-like protein kinase [Benincasa hispida])

HSP 1 Score: 895.2 bits (2312), Expect = 2.6e-256
Identity = 439/488 (89.96%), Postives = 458/488 (93.85%), Query Frame = 0


Query: 1   MRRKYKTLRTTM---RKKMKGALTRHLQVHLGQVSLAPLHLNRASSA-----ASCFQDLP 60
           MRRK +TLRTTM   RKKMKG LTRHLQV LGQVSLAPLHL+R+SS+     A+CFQDLP
Sbjct: 1   MRRKSRTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSSAAASTAACFQDLP 60

Query: 61  ESEAPATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCP 120
           ESEAP TVN SDALPS SPNFEDRDFILSQDFFCTPDYITPDNQNLLNG+D N+E  PCP
Sbjct: 61  ESEAPPTVNASDALPSPSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNQECIPCP 120

Query: 121 KSPEKITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKP 180
           KSPEKI T+KTKRKIH+ +LVHPLSPSLSG EQVVELGNDTFG+DDVDMEKE MAMPKK 
Sbjct: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKQ 180

Query: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240
           NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH
Sbjct: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240

Query: 241 TDFHEIKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSH 300
           TDFHEIKQIGTGNFSRVFKV KRIDGCLYAVKQSTRPL+QD+ERRRALMEVQALAALGSH
Sbjct: 241 TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDIERRRALMEVQALAALGSH 300

Query: 301 ENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGV 360
           ENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPF E DALRAL+QIAKAL FVHEKGV
Sbjct: 301 ENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFFEVDALRALYQIAKALLFVHEKGV 360

Query: 361 AHLDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420
           AHLDVKPDNIYVKDGV+KLGDFGC TLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF
Sbjct: 361 AHLDVKPDNIYVKDGVFKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420

Query: 421 SLGAAIYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEII 480
           SLGAAIYEIVRGSTLP+ HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP RRPSA E++
Sbjct: 421 SLGAAIYEIVRGSTLPEMHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVL 480

[Close]

BLAST of Lag0002524 vs. NCBI nr
Match: XP_022959571.1 (wee1-like protein kinase [Cucurbita moschata])

HSP 1 Score: 891.0 bits (2301), Expect = 4.8e-255
Identity = 435/483 (90.06%), Postives = 452/483 (93.58%), Query Frame = 0


Query: 1   MRRKYKTLRTTM---RKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAP 60
           MRRK KTLRTTM   RKKMKG LTRHLQV LGQVSLAPLH  R+SS A+CFQDLPESEA 
Sbjct: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQRSSSTAACFQDLPESEAM 60

Query: 61  ATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEK 120
           ATVN S ALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNG+DC +E+TPCPKSPEK
Sbjct: 61  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 120

Query: 121 ITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQ 180
           I T+KTKRKI DG+LVHPLSPSLSGREQVVELGNDTFGTDD DMEKE MAMPKK NYVSQ
Sbjct: 121 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 300
           IKQIGTGNFSRVFKV KRIDGCLYAVKQSTRPLHQD+ERRRALMEVQALAALGSHENIVG
Sbjct: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLHQDIERRRALMEVQALAALGSHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDV 360
           YYSSWFENEQLYIQMELCDCSLS GRYSHPFSEADAL+AL QIAKAL F+HEKGV HLDV
Sbjct: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVVHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIF 480
           IYE+VRGST P+ HFMNLKEGKLPL P HSLQFQNLIKAMVDPDPARRPSA E+++NPIF
Sbjct: 421 IYEVVRGSTFPETHFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREVLQNPIF 480

[Close]

BLAST of Lag0002524 vs. NCBI nr
Match: KAG6593183.1 (Wee1-like protein kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 889.8 bits (2298), Expect = 1.1e-254
Identity = 434/483 (89.86%), Postives = 452/483 (93.58%), Query Frame = 0


Query: 1   MRRKYKTLRTTM---RKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAP 60
           MRRK KTLRTTM   RKKMKG LTRHLQV LGQVSLAPLH  R+SS A+CFQDLPESEA 
Sbjct: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQRSSSTAACFQDLPESEAM 60

Query: 61  ATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEK 120
           ATVN S ALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNG+DC +E+TPCPKSPEK
Sbjct: 61  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 120

Query: 121 ITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQ 180
           I T+KTKRKI DG+LVHPLSPSLSGREQVVELGNDTFGTDD DMEKE MAMPKK NYVSQ
Sbjct: 121 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 300
           IKQIGTGNFSRVFKV KRIDGCLYAVKQSTRPLHQD+ERRRALMEVQALAALGSHENIVG
Sbjct: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLHQDIERRRALMEVQALAALGSHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDV 360
           YYSSWFENEQLYIQMELCDCSLS GRYSHPFSEADAL+ L QIAKAL F+HEKGVAHLDV
Sbjct: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKTLHQIAKALLFMHEKGVAHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLD+VDIFSLGAA
Sbjct: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDRVDIFSLGAA 420

Query: 421 IYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIF 480
           IYE+VRGST P+ HFMNLKEGKLPL P HSLQFQNLIKAMVDPDPARRPSA E+++NPIF
Sbjct: 421 IYEVVRGSTFPETHFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREVLQNPIF 480

[Close]

BLAST of Lag0002524 vs. NCBI nr
Match: XP_023514724.1 (wee1-like protein kinase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885.2 bits (2286), Expect = 2.6e-253
Identity = 432/483 (89.44%), Postives = 451/483 (93.37%), Query Frame = 0


Query: 1   MRRKYKTLRTTM---RKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAP 60
           MRRK KTLR TM   RKKMKG LTRHLQV LGQVSLAPLH   +SS A+CFQDLPESEA 
Sbjct: 1   MRRKSKTLRATMTRTRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAM 60

Query: 61  ATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEK 120
           ATVN S ALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNG+DC +E+TPCPKSPEK
Sbjct: 61  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 120

Query: 121 ITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQ 180
           I T+KTKRKI DG+LVHPLSPSLSGREQVVELGNDTFGTDD DMEKE MAMPKK NYVSQ
Sbjct: 121 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPY+ DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYVNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 300
           IK+IGTGNFSRVFKV KRIDGCLYAVKQSTRPLHQD+ERRRALMEVQALAALGSHENIVG
Sbjct: 241 IKKIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLHQDIERRRALMEVQALAALGSHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDV 360
           YYSSWFENEQLYIQMELCDCSLS GRYSHPFSEADAL+AL QIAKAL F+HEKGVAHLDV
Sbjct: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIF 480
           IYE+VRGST P+ HFMNLKEGKLPL P HSLQFQNLIKAMVDPDPARRPSA E+++NPIF
Sbjct: 421 IYEVVRGSTFPETHFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREVLQNPIF 480

[Close]

BLAST of Lag0002524 vs. NCBI nr
Match: XP_023004528.1 (wee1-like protein kinase [Cucurbita maxima])

HSP 1 Score: 884.0 bits (2283), Expect = 5.9e-253
Identity = 434/483 (89.86%), Postives = 450/483 (93.17%), Query Frame = 0


Query: 1   MRRKYKTLRTTM---RKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAP 60
           MRRK KTLRTTM   RKKMKG LTRHLQV LGQVSLAPLH   +SS A+CFQDLPESEA 
Sbjct: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAM 60

Query: 61  ATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEK 120
           ATVN S ALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNG+DC +E+TPCPKSPEK
Sbjct: 61  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 120

Query: 121 ITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQ 180
           I T+KTKRKI DG+LVHPLSPSLSGREQVVELGNDTFGTDD DMEKE MAMPKK NYVSQ
Sbjct: 121 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 300
           IKQIGTGNFSRVFKV KRIDGCLYAVKQSTR LHQD+ERRRALMEVQALAALGSHENIVG
Sbjct: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDV 360
           YYSSWFENEQLYIQMELCDCSLS GRYSHPFSEADAL+AL QIAKAL F+HEKGVAHLDV
Sbjct: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIF 480
           IYE+VRGST P+  FMNLKEGKLPL P HSLQFQNLIKAMVDPDPARRPSA EI++NPIF
Sbjct: 421 IYEVVRGSTFPETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIF 480

[Close]

BLAST of Lag0002524 vs. ExPASy Swiss-Prot
Match: Q8L4H0 (Wee1-like protein kinase OS=Arabidopsis thaliana OX=3702 GN=WEE1 PE=1 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 4.2e-161
Identity = 288/481 (59.88%), Postives = 361/481 (75.05%), Query Frame = 0


Query: 6   KTLRTTMRKKMKGALTRHLQVHLGQVSLAPLHLNRASS--AASCFQDLPESEAPATVNGS 65
           KT  +   +KM+G L RH  +  GQ+S      NR SS  A+S FQ L +S++    N  
Sbjct: 20  KTRASKKIRKMEGTLERHSLLQFGQLSKISFE-NRPSSNVASSAFQGLLDSDSSELRNQL 79

Query: 66  DALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEKITTIKT 125
            +  S + N  ++DFILSQDFFCTPDYITPDNQNL++GLD +++ +PCP+SP K+ T+K+
Sbjct: 80  GSADSDA-NCGEKDFILSQDFFCTPDYITPDNQNLMSGLDISKDHSPCPRSPVKLNTVKS 139

Query: 126 KRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQSAVALR 185
           KR   +    +  + + S + +V E  ND   TD+V  +K      ++  YVSQ+AVALR
Sbjct: 140 KRCRQESFTGNHSNSTWSSKHRVDEQENDDIDTDEVMGDKLQANQTERTGYVSQAAVALR 199

Query: 186 CRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKQIGT 245
           CR MPPPC+KNPY+ + S+   DPFG+QR+KCA F P   SGDGLSRY TDFHEI+QIG 
Sbjct: 200 CRAMPPPCLKNPYVLNQSETATDPFGHQRSKCASFLPVSTSGDGLSRYLTDFHEIRQIGA 259

Query: 246 GNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVGYYSSWF 305
           G+FSRVFKV KR+DGCLYAVK STR L+ D ERR+A+MEVQALAALG HENIVGYYSSWF
Sbjct: 260 GHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQALAALGFHENIVGYYSSWF 319

Query: 306 ENEQLYIQMELCDCSLSI--GRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDVKPDN 365
           ENEQLYIQ+ELCD SLS    + S   SE + L  + QIAKAL FVHEKG+AHLDVKPDN
Sbjct: 320 ENEQLYIQLELCDHSLSALPKKSSLKVSEREILVIMHQIAKALHFVHEKGIAHLDVKPDN 379

Query: 366 IYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 425
           IY+K+GV KLGDFGCAT LDKSLP+EEGDARYMPQEILNE Y++LDKVDIFSLG  +YE+
Sbjct: 380 IYIKNGVCKLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYEHLDKVDIFSLGVTVYEL 439

Query: 426 VRGSTLPQA--HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIFDK 481
           ++GS L ++    +N+KEGKLPLLPGHSLQ Q L+K M+D DP RRPSA E++++P+FD+
Sbjct: 440 IKGSPLTESRNQSLNIKEGKLPLLPGHSLQLQQLLKTMMDRDPKRRPSARELLDHPMFDR 498

[Close]

BLAST of Lag0002524 vs. ExPASy Swiss-Prot
Match: Q6Z829 (Wee1-like protein kinase OS=Oryza sativa subsp. japonica OX=39947 GN=WEE1 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 5.0e-138
Identity = 254/438 (57.99%), Postives = 323/438 (73.74%), Query Frame = 0


Query: 51  LPESEAPATVNGSDALPSSSPNFEDRDF-ILSQDFFCTPDYITPDNQNLLNGLDCNEETT 110
           LP  +  AT   +D +       E++D  ILSQDFFCTPDYITPD   L +G D N+E  
Sbjct: 91  LPPLQPQATPMDADEV------VEEKDSGILSQDFFCTPDYITPDAPQLGSGFDANKENI 150

Query: 111 PCPKSPEKITTIKTKRKIHDGLLVHPLSPSLSGREQVVE---LGNDTF-GTDDVDMEK-E 170
           PCP SPEK +  ++KR   D       SP   G   + +   +    F G DD + E+ +
Sbjct: 151 PCPNSPEK-SVCRSKRYKRD------CSPKGLGSNDIFDSQWIAPVQFEGLDDSEEEQLK 210

Query: 171 AMAMPKKPNYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFS 230
             +  K+ +YVSQSAVALRCRVMPPPC++NPYL     +  + FG ++ K +GF P++  
Sbjct: 211 ESSSHKRGSYVSQSAVALRCRVMPPPCIRNPYLNTDHQIDDNVFGGRQCKSSGFSPSV-D 270

Query: 231 GDGLSRYHTDFHEIKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQ 290
           GDGLSRY TDFHEI+QIG GNFS VFKV KRIDGCLYAVK+S R LH D ERR+A+ EVQ
Sbjct: 271 GDGLSRYRTDFHEIEQIGRGNFSVVFKVLKRIDGCLYAVKRSIRQLHNDRERRQAVKEVQ 330

Query: 291 ALAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKAL 350
           ALAALG HENIVGY++SWFEN+QL+IQMELCD  LS+ R + P    +AL  L+QI K L
Sbjct: 331 ALAALGCHENIVGYFTSWFENKQLFIQMELCDRCLSMDR-NQPLKCGEALELLYQICKGL 390

Query: 351 QFVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYD 410
            F+HE+G+AHLDVKPDNIYV++GVYKLGDFGCATL+D+SL IE+GD+RYMP E+LN++Y+
Sbjct: 391 DFIHERGIAHLDVKPDNIYVRNGVYKLGDFGCATLIDRSLAIEDGDSRYMPPEMLNDKYE 450

Query: 411 YLDKVDIFSLGAAIYEIVRGSTLPQA--HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP 470
           +LDKVDIFSLGAAIYE++RG+ LP +   F +L+EGK+ LLPG  +QFQ+LIK+M+DPDP
Sbjct: 451 HLDKVDIFSLGAAIYELIRGTQLPDSGPQFTSLREGKIALLPGCPMQFQSLIKSMMDPDP 510

Query: 471 ARRPSAWEIIENPIFDKM 481
            RRPSA E++ +PIFDK+
Sbjct: 511 VRRPSAKEVLRHPIFDKL 513

[Close]

BLAST of Lag0002524 vs. ExPASy Swiss-Prot
Match: Q8QGV2 (Wee1-like protein kinase 1-B OS=Xenopus laevis OX=8355 GN=wee1-b PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.3e-49
Identity = 100/277 (36.10%), Postives = 157/277 (56.68%), Query Frame = 0


Query: 229 SRYHTDFHEIKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAA 288
           SRY T+FHE+++IG+G F  VFK  KR+DGC+YA+K+S +PL   V+ + AL EV A A 
Sbjct: 242 SRYATEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAV 301

Query: 289 LGSHENIVGYYSSWFENEQLYIQMELC------DCSLSIGRYSHPFSEADALRALFQIAK 348
           LG H ++V YYS+W E++ + IQ E C      D      R    F+E +    L Q+A+
Sbjct: 302 LGQHPHVVRYYSAWAEDDHMLIQNEYCNGGSLSDVISENYRTMQYFTEPELKDLLLQVAR 361

Query: 349 ALQFVHEKGVAHLDVKPDNIYVK--------------------DGVYKLGDFGCATLLDK 408
            L+++H   + H+D+KP NI++                       +YK+GD G  T +  
Sbjct: 362 GLKYIHSMSLVHMDIKPSNIFISRTTLPNTAVEEADDEECGSGKVIYKIGDLGHVTRV-S 421

Query: 409 SLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVRGSTLPQ--AHFMNLKEGKL 468
           S  +EEGD+R++  E+L E Y +L K DIF+L   ++        P     +  +++GKL
Sbjct: 422 SPQVEEGDSRFLANEVLQENYTHLAKADIFALALTVWSAAGAEPFPTNGDQWHEIRQGKL 481

Query: 469 PLLPG-HSLQFQNLIKAMVDPDPARRPSAWEIIENPI 477
           P +P   S +F +LIK M+ PDP +RPS+  ++++ +
Sbjct: 482 PRVPQLLSQEFVDLIKLMISPDPEKRPSSVALVKHSV 517

[Close]

BLAST of Lag0002524 vs. ExPASy Swiss-Prot
Match: P54350 (Wee1-like protein kinase OS=Drosophila melanogaster OX=7227 GN=Wee1 PE=1 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 4.3e-49
Identity = 106/286 (37.06%), Postives = 155/286 (54.20%), Query Frame = 0


Query: 228 LSRYHTDFHEIKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALA 287
           +SR+  +F ++  IG G F  VF+   R+DGC+YA+K+S +P+      +RAL EV A A
Sbjct: 232 ISRFKREFMQVNVIGVGEFGVVFQCVNRLDGCIYAIKKSKKPVAGSSFEKRALNEVWAHA 291

Query: 288 ALGSHENIVGYYSSWFENEQLYIQMELCD-CSLSIGRYSHPFSEADALRALFQIAKALQF 347
            LG H+N+V YYS+W E++ + IQ E CD  SL      H   EA+    L  + + L++
Sbjct: 292 VLGKHDNVVRYYSAWAEDDHMLIQNEFCDGGSLHARIQDHCLGEAELKIVLMHVIEGLRY 351

Query: 348 VHEKGVAHLDVKPDNIY-----------------VKDG-----------------VYKLG 407
           +H   + H+D+KP+NI+                  KD                   YK+G
Sbjct: 352 IHSNDLVHMDLKPENIFSTMNPNAHKLVEVQPQQTKDDDGMDSVYEELRHSENLVTYKIG 411

Query: 408 DFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVRGSTLPQ--A 467
           D G  T + K   +EEGD RY+P+EIL+E Y  L K DIFSLG  ++E   G  LP+   
Sbjct: 412 DLGHVTSV-KEPYVEEGDCRYLPKEILHEDYSNLFKADIFSLGITLFEAAGGGPLPKNGP 471

Query: 468 HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPI 477
            + NL++GK+P+LP  S  F  LI  M+ P P +RP++  I  +PI
Sbjct: 472 EWHNLRDGKVPILPSLSRDFNELIAQMMHPYPDKRPTSQSIFSHPI 516

[Close]

BLAST of Lag0002524 vs. ExPASy Swiss-Prot
Match: P30291 (Wee1-like protein kinase OS=Homo sapiens OX=9606 GN=WEE1 PE=1 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 5.7e-49
Identity = 100/277 (36.10%), Postives = 159/277 (57.40%), Query Frame = 0


Query: 229 SRYHTDFHEIKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAA 288
           SRY T+FHE+++IG+G F  VFK  KR+DGC+YA+K+S +PL   V+ + AL EV A A 
Sbjct: 293 SRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAV 352

Query: 289 LGSHENIVGYYSSWFENEQLYIQMELC------DCSLSIGRYSHPFSEADALRALFQIAK 348
           LG H ++V Y+S+W E++ + IQ E C      D      R    F EA+    L Q+ +
Sbjct: 353 LGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGR 412

Query: 349 ALQFVHEKGVAHLDVKPDNIYV----------KDG----------VYKLGDFGCATLLDK 408
            L+++H   + H+D+KP NI++          ++G          ++K+GD G  T +  
Sbjct: 413 GLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRI-S 472

Query: 409 SLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVRGSTLPQ--AHFMNLKEGKL 468
           S  +EEGD+R++  E+L E Y +L K DIF+L   +        LP+    +  +++G+L
Sbjct: 473 SPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRL 532

Query: 469 PLLPG-HSLQFQNLIKAMVDPDPARRPSAWEIIENPI 477
           P +P   S +F  L+K M+ PDP RRPSA  ++++ +
Sbjct: 533 PRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSV 568

[Close]

BLAST of Lag0002524 vs. ExPASy TrEMBL
Match: A0A6J1H582 (wee1-like protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111460604 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.3e-255
Identity = 435/483 (90.06%), Postives = 452/483 (93.58%), Query Frame = 0


Query: 1   MRRKYKTLRTTM---RKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAP 60
           MRRK KTLRTTM   RKKMKG LTRHLQV LGQVSLAPLH  R+SS A+CFQDLPESEA 
Sbjct: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQRSSSTAACFQDLPESEAM 60

Query: 61  ATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEK 120
           ATVN S ALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNG+DC +E+TPCPKSPEK
Sbjct: 61  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 120

Query: 121 ITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQ 180
           I T+KTKRKI DG+LVHPLSPSLSGREQVVELGNDTFGTDD DMEKE MAMPKK NYVSQ
Sbjct: 121 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 300
           IKQIGTGNFSRVFKV KRIDGCLYAVKQSTRPLHQD+ERRRALMEVQALAALGSHENIVG
Sbjct: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLHQDIERRRALMEVQALAALGSHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDV 360
           YYSSWFENEQLYIQMELCDCSLS GRYSHPFSEADAL+AL QIAKAL F+HEKGV HLDV
Sbjct: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVVHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIF 480
           IYE+VRGST P+ HFMNLKEGKLPL P HSLQFQNLIKAMVDPDPARRPSA E+++NPIF
Sbjct: 421 IYEVVRGSTFPETHFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREVLQNPIF 480

[Close]

BLAST of Lag0002524 vs. ExPASy TrEMBL
Match: A0A6J1KZT5 (wee1-like protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111497804 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 2.9e-253
Identity = 434/483 (89.86%), Postives = 450/483 (93.17%), Query Frame = 0


Query: 1   MRRKYKTLRTTM---RKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAP 60
           MRRK KTLRTTM   RKKMKG LTRHLQV LGQVSLAPLH   +SS A+CFQDLPESEA 
Sbjct: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAM 60

Query: 61  ATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEK 120
           ATVN S ALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNG+DC +E+TPCPKSPEK
Sbjct: 61  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 120

Query: 121 ITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQ 180
           I T+KTKRKI DG+LVHPLSPSLSGREQVVELGNDTFGTDD DMEKE MAMPKK NYVSQ
Sbjct: 121 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 300
           IKQIGTGNFSRVFKV KRIDGCLYAVKQSTR LHQD+ERRRALMEVQALAALGSHENIVG
Sbjct: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDV 360
           YYSSWFENEQLYIQMELCDCSLS GRYSHPFSEADAL+AL QIAKAL F+HEKGVAHLDV
Sbjct: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIF 480
           IYE+VRGST P+  FMNLKEGKLPL P HSLQFQNLIKAMVDPDPARRPSA EI++NPIF
Sbjct: 421 IYEVVRGSTFPETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIF 480

[Close]

BLAST of Lag0002524 vs. ExPASy TrEMBL
Match: A0A0A0K5B3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G395770 PE=3 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.6e-251
Identity = 427/483 (88.41%), Postives = 450/483 (93.17%), Query Frame = 0


Query: 1   MRRKYKTLRTTM---RKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAP 60
           MRRK KTLRTTM   RKKMKG +TRHLQV LG+VSLAPLHLNR+SS+A CF  LPESE P
Sbjct: 1   MRRKSKTLRTTMGARRKKMKGTVTRHLQVQLGKVSLAPLHLNRSSSSALCFPSLPESEPP 60

Query: 61  ATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEK 120
           +TVN SD LPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNG+D N+E  PCPKSPEK
Sbjct: 61  STVNFSDPLPSSSHNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEK 120

Query: 121 ITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQ 180
           + T+KTKRKIH+ +LV PLSPSLSG EQVVELGND FG+DDVDMEKE  AMPKK NYVSQ
Sbjct: 121 VNTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDIFGSDDVDMEKETTAMPKKENYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 300
           IK IGTGNFSRVFKV KRIDGCLYAVKQSTRPL+QD ERRRALMEVQALAALG+HENIVG
Sbjct: 241 IKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGAHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDV 360
           YY+SWFENEQLYIQMELCDCSLS+GRYSHPFSE DALRAL+QIAKAL FVHEKG+AHLDV
Sbjct: 301 YYTSWFENEQLYIQMELCDCSLSMGRYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIY+KDGVYKLGDFGC TLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIF 480
           IYEIVRG TLP+ HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP+RRPSA E+IENPIF
Sbjct: 421 IYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPSRRPSAREVIENPIF 480

[Close]

BLAST of Lag0002524 vs. ExPASy TrEMBL
Match: A0A1S3BRY6 (wee1-like protein kinase OS=Cucumis melo OX=3656 GN=LOC103492846 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.8e-251
Identity = 427/483 (88.41%), Postives = 453/483 (93.79%), Query Frame = 0


Query: 1   MRRKYKTLRTTM---RKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAP 60
           MRRK KTLRTTM   RKKMKG +TRHLQV LG+VSLAPL L+R+SS+A+CFQ+LPESEAP
Sbjct: 1   MRRKSKTLRTTMGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSSSAACFQNLPESEAP 60

Query: 61  ATVNGSDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEK 120
           +TVN SDALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNG+D N+E  PCPKSPEK
Sbjct: 61  STVNVSDALPSSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEK 120

Query: 121 ITTIKTKRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQ 180
           I T+KTKRKIH+ +LV PLSPSLSG EQVVELGNDTFG+DDVDMEKE MAMPKK +YVSQ
Sbjct: 121 INTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYL DASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLGDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 300
           IK IGTGNFSRVFKV KRIDGCLYAVKQSTRPL+QD ERRRALMEVQALAALGSHENIVG
Sbjct: 241 IKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDV 360
           YY+SWFENEQLYIQMELCDCSLS+G YSHPFSE DALRAL+QIAKAL FVHEKG+AHLDV
Sbjct: 301 YYTSWFENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIY+KDGVYKLGDFGC TLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEIVRGSTLPQAHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIF 480
           IYEIVRG TLP+ HFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+P RRPSA E++ENPIF
Sbjct: 421 IYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVLENPIF 480

[Close]

BLAST of Lag0002524 vs. ExPASy TrEMBL
Match: A0A5A7VN66 (Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G002220 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.0e-247
Identity = 417/470 (88.72%), Postives = 443/470 (94.26%), Query Frame = 0


Query: 11  TMRKKMKGALTRHLQVHLGQVSLAPLHLNRASSAASCFQDLPESEAPATVNGSDALPSSS 70
           T RKKMKG +TRHLQV LG+VSLAPL L+R+SS+A+CFQ+LPESEAP+TVN SDALPSSS
Sbjct: 3   TRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSSSAACFQNLPESEAPSTVNVSDALPSSS 62

Query: 71  PNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEKITTIKTKRKIHDG 130
            NFEDRDFILSQDFFCTPDYITPDNQNLLNG+D N+E  PCPKSPEKI T+KTKRKIH+ 
Sbjct: 63  SNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKTKRKIHEN 122

Query: 131 LLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQSAVALRCRVMPPP 190
           +LV PLSPSLSG EQVVELGNDTFG+DDVDMEKE MAMPKK +YVSQSAVALRCRVMPPP
Sbjct: 123 VLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVSQSAVALRCRVMPPP 182

Query: 191 CMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKQIGTGNFSRVF 250
           CMKNPYL DASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIK IGTGNFSRVF
Sbjct: 183 CMKNPYLGDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKLIGTGNFSRVF 242

Query: 251 KVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVGYYSSWFENEQLYI 310
           KV KRIDGCLYAVKQSTRPL+QD ERRRALMEVQALAALGSHENIVGYY+SWFENEQLYI
Sbjct: 243 KVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVGYYTSWFENEQLYI 302

Query: 311 QMELCDCSLSIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDVKPDNIYVKDGVYK 370
           QMELCDCSLS+G YSHPFSE DALRAL+QIAKAL FVHEKG+AHLDVKPDNIY+KDGVYK
Sbjct: 303 QMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYK 362

Query: 371 LGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVRGSTLPQA 430
           LGDFGC TLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVRG TLP+ 
Sbjct: 363 LGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVRGFTLPET 422

Query: 431 HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIFDKM 481
           HFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+P RRPSA E++ENPIFDK+
Sbjct: 423 HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVLENPIFDKV 472

[Close]

BLAST of Lag0002524 vs. TAIR 10
Match: AT1G02970.1 (WEE1 kinase homolog )

HSP 1 Score: 569.3 bits (1466), Expect = 3.0e-162
Identity = 288/481 (59.88%), Postives = 361/481 (75.05%), Query Frame = 0


Query: 6   KTLRTTMRKKMKGALTRHLQVHLGQVSLAPLHLNRASS--AASCFQDLPESEAPATVNGS 65
           KT  +   +KM+G L RH  +  GQ+S      NR SS  A+S FQ L +S++    N  
Sbjct: 20  KTRASKKIRKMEGTLERHSLLQFGQLSKISFE-NRPSSNVASSAFQGLLDSDSSELRNQL 79

Query: 66  DALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGLDCNEETTPCPKSPEKITTIKT 125
            +  S + N  ++DFILSQDFFCTPDYITPDNQNL++GLD +++ +PCP+SP K+ T+K+
Sbjct: 80  GSADSDA-NCGEKDFILSQDFFCTPDYITPDNQNLMSGLDISKDHSPCPRSPVKLNTVKS 139

Query: 126 KRKIHDGLLVHPLSPSLSGREQVVELGNDTFGTDDVDMEKEAMAMPKKPNYVSQSAVALR 185
           KR   +    +  + + S + +V E  ND   TD+V  +K      ++  YVSQ+AVALR
Sbjct: 140 KRCRQESFTGNHSNSTWSSKHRVDEQENDDIDTDEVMGDKLQANQTERTGYVSQAAVALR 199

Query: 186 CRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKQIGT 245
           CR MPPPC+KNPY+ + S+   DPFG+QR+KCA F P   SGDGLSRY TDFHEI+QIG 
Sbjct: 200 CRAMPPPCLKNPYVLNQSETATDPFGHQRSKCASFLPVSTSGDGLSRYLTDFHEIRQIGA 259

Query: 246 GNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVGYYSSWF 305
           G+FSRVFKV KR+DGCLYAVK STR L+ D ERR+A+MEVQALAALG HENIVGYYSSWF
Sbjct: 260 GHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQALAALGFHENIVGYYSSWF 319

Query: 306 ENEQLYIQMELCDCSLSI--GRYSHPFSEADALRALFQIAKALQFVHEKGVAHLDVKPDN 365
           ENEQLYIQ+ELCD SLS    + S   SE + L  + QIAKAL FVHEKG+AHLDVKPDN
Sbjct: 320 ENEQLYIQLELCDHSLSALPKKSSLKVSEREILVIMHQIAKALHFVHEKGIAHLDVKPDN 379

Query: 366 IYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 425
           IY+K+GV KLGDFGCAT LDKSLP+EEGDARYMPQEILNE Y++LDKVDIFSLG  +YE+
Sbjct: 380 IYIKNGVCKLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYEHLDKVDIFSLGVTVYEL 439

Query: 426 VRGSTLPQA--HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIFDK 481
           ++GS L ++    +N+KEGKLPLLPGHSLQ Q L+K M+D DP RRPSA E++++P+FD+
Sbjct: 440 IKGSPLTESRNQSLNIKEGKLPLLPGHSLQLQQLLKTMMDRDPKRRPSARELLDHPMFDR 498

[Close]

BLAST of Lag0002524 vs. TAIR 10
Match: AT4G19110.1 (Protein kinase superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 5.9e-25
Identity = 80/285 (28.07%), Postives = 137/285 (48.07%), Query Frame = 0


Query: 238 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 297
           IK++G G F  V++   +  G + A+K+  +  +   E    L EV++L  + +H NIV 
Sbjct: 7   IKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYSWDECIN-LREVKSLRRM-NHPNIVK 66

Query: 298 YYSSWFENEQLYIQMELCDCSL--SIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHL 357
                 EN+ LY   E  +C+L   +      F+EAD     FQ+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYMHQRGYFHR 126

Query: 358 DVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 417
           D+KP+N+ V   + K+ DFG A  ++ S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 418 LGAAIYEIVR------GST-----------------------LPQAHFMNLKEGKLP--- 477
           +GA + E++       G++                       L  A+ +N +  +LP   
Sbjct: 187 MGAIMAELLSLRPIFPGASEADEIYKICSVIGTPTEETWLEGLNLANTINYQFPQLPGVP 246

Query: 478 ---LLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIFDKMGFV 484
              L+P  S    NLI+ +   DP+ RP+A E++++P F    +V
Sbjct: 247 LSSLMPSASEDAINLIERLCSWDPSSRPTAAEVLQHPFFQSCFYV 289

[Close]

BLAST of Lag0002524 vs. TAIR 10
Match: AT4G19110.2 (Protein kinase superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 5.9e-25
Identity = 80/285 (28.07%), Postives = 137/285 (48.07%), Query Frame = 0


Query: 238 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 297
           IK++G G F  V++   +  G + A+K+  +  +   E    L EV++L  + +H NIV 
Sbjct: 7   IKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYSWDECIN-LREVKSLRRM-NHPNIVK 66

Query: 298 YYSSWFENEQLYIQMELCDCSL--SIGRYSHPFSEADALRALFQIAKALQFVHEKGVAHL 357
                 EN+ LY   E  +C+L   +      F+EAD     FQ+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYMHQRGYFHR 126

Query: 358 DVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 417
           D+KP+N+ V   + K+ DFG A  ++ S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 418 LGAAIYEIVR------GST-----------------------LPQAHFMNLKEGKLP--- 477
           +GA + E++       G++                       L  A+ +N +  +LP   
Sbjct: 187 MGAIMAELLSLRPIFPGASEADEIYKICSVIGTPTEETWLEGLNLANTINYQFPQLPGVP 246

Query: 478 ---LLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIFDKMGFV 484
              L+P  S    NLI+ +   DP+ RP+A E++++P F    +V
Sbjct: 247 LSSLMPSASEDAINLIERLCSWDPSSRPTAAEVLQHPFFQSCFYV 289

[Close]

BLAST of Lag0002524 vs. TAIR 10
Match: AT5G45430.1 (Protein kinase superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 7.2e-23
Identity = 77/285 (27.02%), Postives = 130/285 (45.61%), Query Frame = 0


Query: 238 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 297
           +K++G G F  V++   +    + A+K+  +  +   E    L EV++L+ + +H NIV 
Sbjct: 7   LKEVGDGTFGNVWRAVNKQTNEVVAIKRMKKK-YFSWEECVNLREVKSLSRM-NHPNIVK 66

Query: 298 YYSSWFENEQLYIQMELCDCSLSIGRYSHP--FSEADALRALFQIAKALQFVHEKGVAHL 357
                 EN+ LY   E  +C+L       P  F+E+D     FQ+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRPKHFAESDIRNWCFQVFQGLSYMHQRGYFHR 126

Query: 358 DVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 417
           D+KP+N+ V   V K+ D G A  +D S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDVIKIADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 418 LGAAIYEI---------------------VRGST--------LPQAHFMNLKEGKLP--- 477
           +GA + E+                     V GS         L  A  +N +  + P   
Sbjct: 187 MGAIMAELLSLRPLFPGASEADEIYKICSVIGSPTEETWLEGLNLASVINYQFPQFPGVH 246

Query: 478 ---LLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIFDKMGFV 484
              ++P  S    NLI+ +   DP  RP+  E +++P F    ++
Sbjct: 247 LSSVMPYASADAVNLIERLCSWDPCNRPTTAEALQHPFFQSCYYI 289

[Close]

BLAST of Lag0002524 vs. TAIR 10
Match: AT5G45430.2 (Protein kinase superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 7.2e-23
Identity = 77/285 (27.02%), Postives = 130/285 (45.61%), Query Frame = 0


Query: 238 IKQIGTGNFSRVFKVFKRIDGCLYAVKQSTRPLHQDVERRRALMEVQALAALGSHENIVG 297
           +K++G G F  V++   +    + A+K+  +  +   E    L EV++L+ + +H NIV 
Sbjct: 7   LKEVGDGTFGNVWRAVNKQTNEVVAIKRMKKK-YFSWEECVNLREVKSLSRM-NHPNIVK 66

Query: 298 YYSSWFENEQLYIQMELCDCSLSIGRYSHP--FSEADALRALFQIAKALQFVHEKGVAHL 357
                 EN+ LY   E  +C+L       P  F+E+D     FQ+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRPKHFAESDIRNWCFQVFQGLSYMHQRGYFHR 126

Query: 358 DVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 417
           D+KP+N+ V   V K+ D G A  +D S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDVIKIADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 418 LGAAIYEI---------------------VRGST--------LPQAHFMNLKEGKLP--- 477
           +GA + E+                     V GS         L  A  +N +  + P   
Sbjct: 187 MGAIMAELLSLRPLFPGASEADEIYKICSVIGSPTEETWLEGLNLASVINYQFPQFPGVH 246

Query: 478 ---LLPGHSLQFQNLIKAMVDPDPARRPSAWEIIENPIFDKMGFV 484
              ++P  S    NLI+ +   DP  RP+  E +++P F    ++
Sbjct: 247 LSSVMPYASADAVNLIERLCSWDPCNRPTTAEALQHPFFQSCYYI 289

The following BLAST results are available for this feature:

BLAST of Lag0002524 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876752.1	2.6e-256	89.96	wee1-like protein kinase [Benincasa hispida]	[more]
XP_022959571.1	4.8e-255	90.06	wee1-like protein kinase [Cucurbita moschata]	[more]
KAG6593183.1	1.1e-254	89.86	Wee1-like protein kinase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023514724.1	2.6e-253	89.44	wee1-like protein kinase [Cucurbita pepo subsp. pepo]	[more]
XP_023004528.1	5.9e-253	89.86	wee1-like protein kinase [Cucurbita maxima]	[more]

BLAST of Lag0002524 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L4H0	4.2e-161	59.88	Wee1-like protein kinase OS=Arabidopsis thaliana OX=3702 GN=WEE1 PE=1 SV=1	[more]
Q6Z829	5.0e-138	57.99	Wee1-like protein kinase OS=Oryza sativa subsp. japonica OX=39947 GN=WEE1 PE=3 S...	[more]
Q8QGV2	4.3e-49	36.10	Wee1-like protein kinase 1-B OS=Xenopus laevis OX=8355 GN=wee1-b PE=1 SV=1	[more]
P54350	4.3e-49	37.06	Wee1-like protein kinase OS=Drosophila melanogaster OX=7227 GN=Wee1 PE=1 SV=2	[more]
P30291	5.7e-49	36.10	Wee1-like protein kinase OS=Homo sapiens OX=9606 GN=WEE1 PE=1 SV=2	[more]

BLAST of Lag0002524 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H582	2.3e-255	90.06	wee1-like protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111460604 PE=3 SV=1	[more]
A0A6J1KZT5	2.9e-253	89.86	wee1-like protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111497804 PE=3 SV=1	[more]
A0A0A0K5B3	4.6e-251	88.41	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G395...	[more]
A0A1S3BRY6	7.8e-251	88.41	wee1-like protein kinase OS=Cucumis melo OX=3656 GN=LOC103492846 PE=3 SV=1	[more]
A0A5A7VN66	1.0e-247	88.72	Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]

BLAST of Lag0002524 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02970.1	3.0e-162	59.88	WEE1 kinase homolog	[more]
AT4G19110.1	5.9e-25	28.07	Protein kinase superfamily protein	[more]
AT4G19110.2	5.9e-25	28.07	Protein kinase superfamily protein	[more]
AT5G45430.1	7.2e-23	27.02	Protein kinase superfamily protein	[more]
AT5G45430.2	7.2e-23	27.02	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 235..477 e-value: 1.1E-44 score: 164.5
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 235..477 e-value: 2.4E-47 score: 161.5
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 235..477 score: 36.371742
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 315..481 e-value: 1.6E-36 score: 127.5
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 225..314 e-value: 1.9E-31 score: 110.0
None	No IPR available	PANTHER	PTHR11042:SF155	BNAA08G27310D PROTEIN	coord: 16..393
None	No IPR available	PANTHER	PTHR11042:SF155	BNAA08G27310D PROTEIN	coord: 406..480
None	No IPR available	PANTHER	PTHR11042	EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED	coord: 406..480
None	No IPR available	PANTHER	PTHR11042	EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED	coord: 16..393
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 241..264
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 352..364
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 228..488

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002524.1	Lag0002524.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1902750	negative regulation of cell cycle G2/M phase transition
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004713	protein tyrosine kinase activity
molecular_function	GO:0004672	protein kinase activity