Lag0002521 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002521
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	nuclear transcription factor Y subunit B-1
Location	chr4: 43556351 .. 43556830 (+)
RNA-Seq Expression	Lag0002521
Synteny	Lag0002521

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGATAACACAGGCAAGCAAGAGAGATTAGTAGAATTTAAGTACGATTTCAGCACCGGAGGCGGCGGAGGAGTTGGTGGCAGCACCGGTGGATCGAGCGAGGAAGCCGGTAGCGGTGTCGGTGTCGTCGTAAAAGAGCAAGATCGGTTGCTGCCAATAGCAAACGTGGGGAGGATCATGAAGCAAATTCTGCCTCCGAACGCGAAAATCTCGAAAGAAGCGAAAGAAACTATGCAAGAATGCGTGTCGGAGTTCATCAGCTTCGTCACCGGGGAGGCGTCGGATAAGTGCCATAAGGAGAAGAGGAAGACTGTTAATGGAGATGACATTTGCTGTGCCTTGGCCACACTTGGATTTGATGATTATGCTGAGCCTTTGAGGAGGTATTTGGTTAGGTATAGAGAAATAGAAGGGGAGAGAGCTCAACAAAATAAGGGATGTTGCCATAATAATAGTAGTAATAATCATGATGCATGA

mRNA sequence

ATGGATGATAACACAGGCAAGCAAGAGAGATTAGTAGAATTTAAGTACGATTTCAGCACCGGAGGCGGCGGAGGAGTTGGTGGCAGCACCGGTGGATCGAGCGAGGAAGCCGGTAGCGGTGTCGGTGTCGTCGTAAAAGAGCAAGATCGGTTGCTGCCAATAGCAAACGTGGGGAGGATCATGAAGCAAATTCTGCCTCCGAACGCGAAAATCTCGAAAGAAGCGAAAGAAACTATGCAAGAATGCGTGTCGGAGTTCATCAGCTTCGTCACCGGGGAGGCGTCGGATAAGTGCCATAAGGAGAAGAGGAAGACTGTTAATGGAGATGACATTTGCTGTGCCTTGGCCACACTTGGATTTGATGATTATGCTGAGCCTTTGAGGAGGTATTTGGTTAGGTATAGAGAAATAGAAGGGGAGAGAGCTCAACAAAATAAGGGATGTTGCCATAATAATAGTAGTAATAATCATGATGCATGA

Coding sequence (CDS)

ATGGATGATAACACAGGCAAGCAAGAGAGATTAGTAGAATTTAAGTACGATTTCAGCACCGGAGGCGGCGGAGGAGTTGGTGGCAGCACCGGTGGATCGAGCGAGGAAGCCGGTAGCGGTGTCGGTGTCGTCGTAAAAGAGCAAGATCGGTTGCTGCCAATAGCAAACGTGGGGAGGATCATGAAGCAAATTCTGCCTCCGAACGCGAAAATCTCGAAAGAAGCGAAAGAAACTATGCAAGAATGCGTGTCGGAGTTCATCAGCTTCGTCACCGGGGAGGCGTCGGATAAGTGCCATAAGGAGAAGAGGAAGACTGTTAATGGAGATGACATTTGCTGTGCCTTGGCCACACTTGGATTTGATGATTATGCTGAGCCTTTGAGGAGGTATTTGGTTAGGTATAGAGAAATAGAAGGGGAGAGAGCTCAACAAAATAAGGGATGTTGCCATAATAATAGTAGTAATAATCATGATGCATGA

Protein sequence

MDDNTGKQERLVEFKYDFSTGGGGGVGGSTGGSSEEAGSGVGVVVKEQDRLLPIANVGRIMKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGFDDYAEPLRRYLVRYREIEGERAQQNKGCCHNNSSNNHDA

Homology

[Close]

BLAST of Lag0002521 vs. NCBI nr
Match: XP_023549145.1 (nuclear transcription factor Y subunit B-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 290.0 bits (741), Expect = 1.2e-74
Identity = 146/159 (91.82%), Postives = 151/159 (94.97%), Query Frame = 0


Query: 1   MDDNTGKQERLVEFKYDFSTGGGGGVGGSTGGSSEEAGSGVGVVVKEQDRLLPIANVGRI 60
           MDDNT   ERLVEFKYDF+TGGGGGVGGSTGGSSEEAGSG+G  VKEQDRLLPIANVGRI
Sbjct: 1   MDDNTPMPERLVEFKYDFTTGGGGGVGGSTGGSSEEAGSGIGGFVKEQDRLLPIANVGRI 60

Query: 61  MKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGF 120
           MKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGF
Sbjct: 61  MKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGF 120

Query: 121 DDYAEPLRRYLVRYREIEGERAQQNKGCCHNNSSNNHDA 160
           DDYAEPLRRYL+RYREIEGERAQQNKGCC+NN+   HDA
Sbjct: 121 DDYAEPLRRYLIRYREIEGERAQQNKGCCNNNT---HDA 156

[Close]

BLAST of Lag0002521 vs. NCBI nr
Match: XP_022991512.1 (nuclear transcription factor Y subunit B-1 [Cucurbita maxima])

HSP 1 Score: 290.0 bits (741), Expect = 1.2e-74
Identity = 146/159 (91.82%), Postives = 151/159 (94.97%), Query Frame = 0


Query: 1   MDDNTGKQERLVEFKYDFSTGGGGGVGGSTGGSSEEAGSGVGVVVKEQDRLLPIANVGRI 60
           MDDNT   ERLVEFKYDF+TGGGGGVGGSTGGSSEEAGSG+G  VKEQDRLLPIANVGRI
Sbjct: 1   MDDNTPMPERLVEFKYDFTTGGGGGVGGSTGGSSEEAGSGIGGFVKEQDRLLPIANVGRI 60

Query: 61  MKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGF 120
           MKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGF
Sbjct: 61  MKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGF 120

Query: 121 DDYAEPLRRYLVRYREIEGERAQQNKGCCHNNSSNNHDA 160
           DDYAEPLRRYL+RYREIEGERAQQNKGCC+NN+   HDA
Sbjct: 121 DDYAEPLRRYLIRYREIEGERAQQNKGCCNNNA---HDA 156

[Close]

BLAST of Lag0002521 vs. NCBI nr
Match: XP_038876756.1 (nuclear transcription factor Y subunit B-1-like [Benincasa hispida])

HSP 1 Score: 286.6 bits (732), Expect = 1.3e-73
Identity = 145/158 (91.77%), Postives = 150/158 (94.94%), Query Frame = 0


Query: 1   MDDNTGKQERLVEFKYDFSTGGGGGVGGSTGGSSEEAGSGVGVVVKEQDRLLPIANVGRI 60
           MD+NTG  ERLVEFKYDF+ GGG GVGGSTGGSSEEAG+GVG VVKEQDRLLPIANVGRI
Sbjct: 1   MDENTGMPERLVEFKYDFTGGGGSGVGGSTGGSSEEAGNGVGGVVKEQDRLLPIANVGRI 60

Query: 61  MKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGF 120
           MKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGF
Sbjct: 61  MKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGF 120

Query: 121 DDYAEPLRRYLVRYREIEGERAQQNKGCCHNNSSNNHD 159
           DDYAEPLRRYLVRYRE+EGERAQQNKGCC+N   NNHD
Sbjct: 121 DDYAEPLRRYLVRYREMEGERAQQNKGCCNN---NNHD 155

[Close]

BLAST of Lag0002521 vs. NCBI nr
Match: XP_022953161.1 (nuclear transcription factor Y subunit B-1-like [Cucurbita moschata])

HSP 1 Score: 285.4 bits (729), Expect = 3.0e-73
Identity = 146/160 (91.25%), Postives = 151/160 (94.38%), Query Frame = 0


Query: 1   MDDNTGKQERLVEFKYDFSTGGG-GGVGGSTGGSSEEAGSGVGVVVKEQDRLLPIANVGR 60
           MDDNT   ERLVEFKYDF+TGGG GGVGGSTGGSSEEAGSG+G  VKEQDRLLPIANVGR
Sbjct: 1   MDDNTPMPERLVEFKYDFTTGGGSGGVGGSTGGSSEEAGSGIGGFVKEQDRLLPIANVGR 60

Query: 61  IMKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLG 120
           IMKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLG
Sbjct: 61  IMKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLG 120

Query: 121 FDDYAEPLRRYLVRYREIEGERAQQNKGCCHNNSSNNHDA 160
           FDDYAEPLRRYL+RYREIEGERAQQNKGCC+NN+   HDA
Sbjct: 121 FDDYAEPLRRYLIRYREIEGERAQQNKGCCNNNT---HDA 157

[Close]

BLAST of Lag0002521 vs. NCBI nr
Match: KAG6575846.1 (Nuclear transcription factor Y subunit B-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 284.6 bits (727), Expect = 5.1e-73
Identity = 146/162 (90.12%), Postives = 151/162 (93.21%), Query Frame = 0


Query: 1   MDDNTGKQERLVEFKYDFST---GGGGGVGGSTGGSSEEAGSGVGVVVKEQDRLLPIANV 60
           MDDNT   ERLVEFKYDF+T   GGGGGVGGSTGGSSEEAGSG+G  VKEQDRLLPIANV
Sbjct: 1   MDDNTPMPERLVEFKYDFTTGGGGGGGGVGGSTGGSSEEAGSGIGGFVKEQDRLLPIANV 60

Query: 61  GRIMKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALAT 120
           GRIMKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALAT
Sbjct: 61  GRIMKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALAT 120

Query: 121 LGFDDYAEPLRRYLVRYREIEGERAQQNKGCCHNNSSNNHDA 160
           LGFDDYAEPLRRYL+RYREIEGERAQQNKGCC+NN+   HDA
Sbjct: 121 LGFDDYAEPLRRYLIRYREIEGERAQQNKGCCNNNT---HDA 159

[Close]

BLAST of Lag0002521 vs. ExPASy Swiss-Prot
Match: O82248 (Nuclear transcription factor Y subunit B-5 OS=Arabidopsis thaliana OX=3702 GN=NFYB5 PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.8e-42
Identity = 83/99 (83.84%), Postives = 91/99 (91.92%), Query Frame = 0


Query: 43  VVVKEQDRLLPIANVGRIMKQILPPNAKISKEAKETMQECVSEFISFVTGEASDKCHKEK 102
           ++VKEQDRLLPIANVGRIMK ILP NAK+SKEAKETMQECVSEFISFVTGEASDKCHKEK
Sbjct: 47  MMVKEQDRLLPIANVGRIMKNILPANAKVSKEAKETMQECVSEFISFVTGEASDKCHKEK 106

Query: 103 RKTVNGDDICCALATLGFDDYAEPLRRYLVRYREIEGER 142
           RKTVNGDDIC A+A LGFDDYA  L++YL RYR +EGE+
Sbjct: 107 RKTVNGDDICWAMANLGFDDYAAQLKKYLHRYRVLEGEK 145

[Close]

BLAST of Lag0002521 vs. ExPASy Swiss-Prot
Match: Q9SLG0 (Nuclear transcription factor Y subunit B-1 OS=Arabidopsis thaliana OX=3702 GN=NFYB1 PE=1 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 7.2e-38
Identity = 83/135 (61.48%), Postives = 99/135 (73.33%), Query Frame = 0


Query: 25  GVGGSTGGSSEEAGSGVGVVVKEQDRLLPIANVGRIMKQILPPNAKISKEAKETMQECVS 84
           G GG +GGS           V+EQDR LPIAN+ RIMK+ LPPN KI K+AK+T+QECVS
Sbjct: 10  GDGGESGGS-----------VREQDRYLPIANISRIMKKALPPNGKIGKDAKDTVQECVS 69

Query: 85  EFISFVTGEASDKCHKEKRKTVNGDDICCALATLGFDDYAEPLRRYLVRYREIEGERAQQ 144
           EFISF+T EASDKC KEKRKTVNGDD+  A+ATLGF+DY EPL+ YL RYRE+EG+    
Sbjct: 70  EFISFITSEASDKCQKEKRKTVNGDDLLWAMATLGFEDYLEPLKIYLARYRELEGD---- 129

Query: 145 NKGCCHNNSSNNHDA 160
           NKG   +   +N DA
Sbjct: 130 NKGSGKSGDGSNRDA 129

[Close]

BLAST of Lag0002521 vs. ExPASy Swiss-Prot
Match: Q9FGJ3 (Nuclear transcription factor Y subunit B-2 OS=Arabidopsis thaliana OX=3702 GN=NFYB2 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 7.2e-38
Identity = 80/115 (69.57%), Postives = 93/115 (80.87%), Query Frame = 0


Query: 27  GGSTGGSSEEAGSGVGVVVKEQDRLLPIANVGRIMKQILPPNAKISKEAKETMQECVSEF 86
           GG   G+++   S +    +EQDR LPIANV RIMK+ LP NAKISK+AKETMQECVSEF
Sbjct: 9   GGGQNGNNQNGQSSLS--PREQDRFLPIANVSRIMKKALPANAKISKDAKETMQECVSEF 68

Query: 87  ISFVTGEASDKCHKEKRKTVNGDDICCALATLGFDDYAEPLRRYLVRYREIEGER 142
           ISFVTGEASDKC KEKRKT+NGDD+  A+ TLGF+DY EPL+ YL R+REIEGER
Sbjct: 69  ISFVTGEASDKCQKEKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQRFREIEGER 121

[Close]

BLAST of Lag0002521 vs. ExPASy Swiss-Prot
Match: Q0J7P4 (Nuclear transcription factor Y subunit B-11 OS=Oryza sativa subsp. japonica OX=39947 GN=HD5 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.1e-37
Identity = 82/124 (66.13%), Postives = 93/124 (75.00%), Query Frame = 0


Query: 19  STGGGGGVGGSTGGSSEEAGSGVGVVVKEQDRLLPIANVGRIMKQILPPNAKISKEAKET 78
           +  GGGG G S G      G G     KEQDR LPIANV RIMK+ LP NAKISKE+KET
Sbjct: 30  AAAGGGGCGSSAGYVVYGGGGGGDSPAKEQDRFLPIANVSRIMKRSLPANAKISKESKET 89

Query: 79  MQECVSEFISFVTGEASDKCHKEKRKTVNGDDICCALATLGFDDYAEPLRRYLVRYREIE 138
           +QECVSEFISFVTGEASDKC +EKRKT+NGDD+  A+ TLGF+ Y  PL+ YL RYRE E
Sbjct: 90  VQECVSEFISFVTGEASDKCQREKRKTINGDDLLWAMTTLGFEAYVGPLKSYLNRYREAE 149

Query: 139 GERA 143
           GE+A
Sbjct: 150 GEKA 153

[Close]

BLAST of Lag0002521 vs. ExPASy Swiss-Prot
Match: Q75IZ7 (Nuclear transcription factor Y subunit B-8 OS=Oryza sativa subsp. japonica OX=39947 GN=NFYB8 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 3.6e-37
Identity = 77/113 (68.14%), Postives = 93/113 (82.30%), Query Frame = 0


Query: 30  TGGSSEEAGSGVGVVVKEQDRLLPIANVGRIMKQILPPNAKISKEAKETMQECVSEFISF 89
           +GG S  AG  +    +EQDR LPIANV RIMK+ LP NAKISK+AKET+QECVSEFISF
Sbjct: 8   SGGPSNYAGGELS-SPREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISF 67

Query: 90  VTGEASDKCHKEKRKTVNGDDICCALATLGFDDYAEPLRRYLVRYREIEGERA 143
           +TGEASDKC +EKRKT+NGDD+  A+ TLGF+DY +PL+ YL ++REIEGERA
Sbjct: 68  ITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYVDPLKHYLHKFREIEGERA 119

The following BLAST results are available for this feature:

BLAST of Lag0002521 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023549145.1	1.2e-74	91.82	nuclear transcription factor Y subunit B-1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022991512.1	1.2e-74	91.82	nuclear transcription factor Y subunit B-1 [Cucurbita maxima]	[more]
XP_038876756.1	1.3e-73	91.77	nuclear transcription factor Y subunit B-1-like [Benincasa hispida]	[more]
XP_022953161.1	3.0e-73	91.25	nuclear transcription factor Y subunit B-1-like [Cucurbita moschata]	[more]
KAG6575846.1	5.1e-73	90.12	Nuclear transcription factor Y subunit B-5, partial [Cucurbita argyrosperma subs...	[more]

BLAST of Lag0002521 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82248	2.8e-42	83.84	Nuclear transcription factor Y subunit B-5 OS=Arabidopsis thaliana OX=3702 GN=NF...	[more]
Q9SLG0	7.2e-38	61.48	Nuclear transcription factor Y subunit B-1 OS=Arabidopsis thaliana OX=3702 GN=NF...	[more]
Q9FGJ3	7.2e-38	69.57	Nuclear transcription factor Y subunit B-2 OS=Arabidopsis thaliana OX=3702 GN=NF...	[more]
Q0J7P4	2.1e-37	66.13	Nuclear transcription factor Y subunit B-11 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q75IZ7	3.6e-37	68.14	Nuclear transcription factor Y subunit B-8 OS=Oryza sativa subsp. japonica OX=39...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00615	CCAATSUBUNTA	coord: 117..135 score: 34.09 coord: 79..97 score: 51.32 coord: 98..116 score: 34.21
None	No IPR available	PANTHER	PTHR11064:SF106	NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-5	coord: 36..156
IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain	PFAM	PF00808	CBFD_NFYB_HMF	coord: 51..115 e-value: 6.7E-27 score: 93.5
IPR009072	Histone-fold	GENE3D	1.10.20.10	Histone, subunit A	coord: 44..159 e-value: 1.3E-43 score: 150.2
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 48..156
IPR027113	Transcription factor NFYB/HAP3	PANTHER	PTHR11064	CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED	coord: 36..156
IPR003956	Transcription factor, NFYB/HAP3, conserved site	PROSITE	PS00685	NFYB_HAP3	coord: 82..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002521.1	Lag0002521.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045944	positive regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0016602	CCAAT-binding factor complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0001228	DNA-binding transcription activator activity, RNA polymerase II-specific
molecular_function	GO:0046982	protein heterodimerization activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding