Lag0002518 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002518 vs. NCBI nr
Match: XP_038876738.1 (metal tolerance protein 2 [Benincasa hispida])

HSP 1 Score: 918.7 bits (2373), Expect = 2.2e-263
Identity = 465/499 (93.19%), Postives = 479/499 (95.99%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           MGYRFHRLNP+LQSFY++LSS TH+EFHAIPSIQSL LHPQTTLLGIYDDP SKICRRWH
Sbjct: 6   MGYRFHRLNPVLQSFYTKLSSPTHKEFHAIPSIQSLNLHPQTTLLGIYDDPTSKICRRWH 65

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHSHRHDD HRFG++GENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL
Sbjct: 66  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 125

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAG+APKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA
Sbjct: 126 SGIALWSFKAGQAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 185

Query: 181 PEIVNQPLGHDNLHNHS--HGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQ 240
           PEIVNQP GH++LHNHS  HGGHHHGIDMDHPILALNM IISICIKEGLYW+TKRAGEKQ
Sbjct: 186 PEIVNQPFGHESLHNHSHNHGGHHHGIDMDHPILALNMTIISICIKEGLYWMTKRAGEKQ 245

Query: 241 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSI 300
           GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSI
Sbjct: 246 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSI 305

Query: 301 LELVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC 360
           LELVDAAIPADQINPV+QTIL VEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC
Sbjct: 306 LELVDAAIPADQINPVKQTILEVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC 365

Query: 361 IGENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQNTDVPLNEKNIEA 420
           IGENVRQKIHTSHPEVSEVFIHIDPSISHFPP LS+QQ GST T +QNTD PL EKNIEA
Sbjct: 366 IGENVRQKIHTSHPEVSEVFIHIDPSISHFPPKLSDQQAGSTGTSNQNTDFPLIEKNIEA 425

Query: 421 IVSIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAA 480
           IVS II S FPE MLVERITPHLLQGKILLQIEVSMPP LLIRNAME+AKQAEMEILKAA
Sbjct: 426 IVSEIILSKFPENMLVERITPHLLQGKILLQIEVSMPPNLLIRNAMEIAKQAEMEILKAA 485

Query: 481 SNIVHVSIQLRLGHQIPQL 498
           SNIVHVSIQLRLGHQIPQL
Sbjct: 486 SNIVHVSIQLRLGHQIPQL 504

BLAST of Lag0002518 vs. NCBI nr
Match: KAG6575848.1 (Metal tolerance protein C1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 888.6 bits (2295), Expect = 2.4e-254
Identity = 452/498 (90.76%), Postives = 469/498 (94.18%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           MGYRFHRLNPILQSFYSRLSS+TH+EFHAIPS QS  LHPQ TLLGIY DPKSKICRRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRLSSATHKEFHAIPSTQSFDLHPQATLLGIYGDPKSKICRRWH 60

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHSHRHDD HRFG++GENIFKLGLGADIGLA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH++LHNHSHGGHHHGIDMDHP+LALNM IISI IKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMDHPLLALNMTIISISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TIL VEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQN-TDVPLNEKNIEAI 420
           ENVRQ+IHTSHPE SEVFIHIDPSISH P  LS+QQGGS  T +QN  D+ L EKNIEAI
Sbjct: 361 ENVRQRIHTSHPEASEVFIHIDPSISHVPLKLSDQQGGSAGTSNQNIDDIALEEKNIEAI 420

Query: 421 VSIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAAS 480
           VS II S F E M+VERITPHLLQGKILLQIEVSMPP+LLIRNAMEVAKQAE+E+LKAAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMEVAKQAEIEVLKAAS 480

Query: 481 NIVHVSIQLRLGHQIPQL 498
           NIVHVSI LRLGHQIP L
Sbjct: 481 NIVHVSILLRLGHQIPHL 498

BLAST of Lag0002518 vs. NCBI nr
Match: XP_023548946.1 (metal tolerance protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885.6 bits (2287), Expect = 2.0e-253
Identity = 450/498 (90.36%), Postives = 470/498 (94.38%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           MGYRFHRLNPILQSFYSRLSS++H+EFHAIPS QS  LHPQTTLLGIY DPKSKICRRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRLSSASHKEFHAIPSTQSFDLHPQTTLLGIYGDPKSKICRRWH 60

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHSHRHDD HRFG++GENIFKLGLGADIGLA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH++LHNHSHGGHHHGIDM+HP+LALNM IISI IKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMNHPLLALNMTIISISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TIL VEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQNT-DVPLNEKNIEAI 420
           ENVRQ+IHTSHPE SEVFIHIDPS+SH P  LS+QQGGS  T +QN  D+ L EKNIEAI
Sbjct: 361 ENVRQQIHTSHPEASEVFIHIDPSMSHVPLKLSDQQGGSAGTSNQNIGDIALEEKNIEAI 420

Query: 421 VSIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAAS 480
           VS II S F E M+VERITPHLLQGKILLQIEVSMPP+LLIRNAMEVAKQAE+E+LKAAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMEVAKQAEIEVLKAAS 480

Query: 481 NIVHVSIQLRLGHQIPQL 498
           NIVHVSI LRLGHQIP L
Sbjct: 481 NIVHVSILLRLGHQIPHL 498

BLAST of Lag0002518 vs. NCBI nr
Match: XP_022954108.1 (metal tolerance protein 2 [Cucurbita moschata])

HSP 1 Score: 884.0 bits (2283), Expect = 5.9e-253
Identity = 450/498 (90.36%), Postives = 467/498 (93.78%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           MGYRFHRLNPILQSFYSRLS +TH+EFHAIPS QS  LHPQ TLLGIY DPKSKICRRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRLSLATHKEFHAIPSTQSFDLHPQATLLGIYGDPKSKICRRWH 60

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHSHRHDD HRFG++GENIFKLGLGADIGLA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH++LHNHSHGGHHHGIDMDHPILALNM I+SI IKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMDHPILALNMTILSISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TIL VEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQN-TDVPLNEKNIEAI 420
           ENVRQ+IHTSHPE SEVFIHIDPSISH P  LS+QQGGS  T +QN  D+ L EKNIEAI
Sbjct: 361 ENVRQRIHTSHPEASEVFIHIDPSISHVPLKLSDQQGGSAGTSNQNIDDIALEEKNIEAI 420

Query: 421 VSIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAAS 480
           VS II S F E M+VERITPHLLQGKILLQIEVSMPP+LLIRNAMEVAKQAE+E+L AAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMEVAKQAEIEVLMAAS 480

Query: 481 NIVHVSIQLRLGHQIPQL 498
           NIVHVSI LRLGHQIP L
Sbjct: 481 NIVHVSILLRLGHQIPHL 498

BLAST of Lag0002518 vs. NCBI nr
Match: XP_022991511.1 (metal tolerance protein 2 [Cucurbita maxima])

HSP 1 Score: 880.2 bits (2273), Expect = 8.6e-252
Identity = 448/498 (89.96%), Postives = 467/498 (93.78%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           MGYRFHRLNPILQSF+SRLSS++H+EFHAIPS QS  LHPQTTLLGIY DPKSKICRRWH
Sbjct: 1   MGYRFHRLNPILQSFHSRLSSASHKEFHAIPSTQSFDLHPQTTLLGIYGDPKSKICRRWH 60

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHSHRHDD HRFG++GENIFKLGLGADIGLA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH++LHNHSHGGHHHGIDMDHPILALNM IISI IKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMDHPILALNMTIISISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TIL VEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQN-TDVPLNEKNIEAI 420
           ENVRQ+IHTSHPE SEVFIHIDPSISH P   S+QQGG+  T +QN  D+ L EKNIEAI
Sbjct: 361 ENVRQQIHTSHPEASEVFIHIDPSISHVPLKFSDQQGGTAGTSNQNIDDIALEEKNIEAI 420

Query: 421 VSIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAAS 480
           VS II S F E M+VERITPHLLQ KILLQIEVSMPP+LLIRNAMEVAKQAE+E+ KAAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQSKILLQIEVSMPPDLLIRNAMEVAKQAEIEVSKAAS 480

Query: 481 NIVHVSIQLRLGHQIPQL 498
           NIVHVSI LRLGHQIP L
Sbjct: 481 NIVHVSILLRLGHQIPHL 498

BLAST of Lag0002518 vs. ExPASy Swiss-Prot
Match: Q8L725 (Metal tolerance protein C1 OS=Arabidopsis thaliana OX=3702 GN=MTPC1 PE=2 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.4e-156
Identity = 302/492 (61.38%), Postives = 356/492 (72.36%), Query Frame = 0

Query: 4   RFHRLNPILQSFYSRLSSST--HQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWHL 63
           RF  LNP      +R+  ST  +     +PS  S    P          P     RRWH 
Sbjct: 5   RFQILNP------TRICRSTIYYMSCRYLPS-SSSSSSPLRVSSSSSQKPSFDFSRRWHF 64

Query: 64  GHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVLS 123
           GH   H  + + GE+GE IF+LGL ADIGL+  KA+TGYL GSTAIIADAAHSVSDVVLS
Sbjct: 65  GHPDHHQQYQKPGEEGEKIFRLGLTADIGLSVAKALTGYLCGSTAIIADAAHSVSDVVLS 124

Query: 124 GIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAAP 183
           G+AL S++A   PKDKEHPYGHGKFETLGALGIS+MLLAT  GIAWHA +LL   LSAAP
Sbjct: 125 GVALVSYRAANVPKDKEHPYGHGKFETLGALGISAMLLATGSGIAWHALDLLSIALSAAP 184

Query: 184 EIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGSG 243
           E++             H GHHHGIDM+HPILAL + I SI IKEGLYWITKRAGEKQGSG
Sbjct: 185 EVI-------------HSGHHHGIDMNHPILALTVTIASISIKEGLYWITKRAGEKQGSG 244

Query: 244 LMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILEL 303
           LM ANAWHHR+DAISS+VAL+GVGGSILGV FLDPLAGLVVS MI+ AGL+TG+QSILEL
Sbjct: 245 LMMANAWHHRSDAISSLVALVGVGGSILGVNFLDPLAGLVVSTMIVNAGLKTGHQSILEL 304

Query: 304 VDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIGE 363
           VDAAIPA Q+ P+RQTIL VEGVKGCHRLRGRRAGSSLYLDVHI VDPF SVS AH +GE
Sbjct: 305 VDAAIPAQQLEPIRQTILQVEGVKGCHRLRGRRAGSSLYLDVHIVVDPFSSVSVAHEVGE 364

Query: 364 NVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQNTDVPLNEKNIEAIVS 423
            VR++I+ +HPEVSEVFIHIDP+   F  +  +      +++ + +++    K++EA VS
Sbjct: 365 YVRRQINLNHPEVSEVFIHIDPAFLQFSCSTKDH-----DSITKESNICQEIKHVEATVS 424

Query: 424 IIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAASNI 483
            I  S   EK+ ++RITPHLL  KILLQI V+MP  + I++ M  A+ AE EILKAA N+
Sbjct: 425 DIFSSQLSEKLTIKRITPHLLHSKILLQIVVAMPSTMSIQDVMIAAEHAEKEILKAAPNV 471

Query: 484 VHVSIQLRLGHQ 494
             VSIQL L  +
Sbjct: 485 ARVSIQLSLNSE 471

BLAST of Lag0002518 vs. ExPASy Swiss-Prot
Match: Q10LJ2 (Metal tolerance protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP2 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 6.6e-130
Identity = 234/327 (71.56%), Postives = 277/327 (84.71%), Query Frame = 0

Query: 58  RWHLGHSHRHDDHHR---FGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHS 117
           R H+GHSH H  HH     GE  E IF+LGL AD+ L  GKAVTGYLSGSTAI ADAAHS
Sbjct: 56  RGHVGHSHHH--HHGEEVGGEASERIFRLGLAADVVLTVGKAVTGYLSGSTAIAADAAHS 115

Query: 118 VSDVVLSGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLL 177
           +SD+VLSG+AL S+KA KAP+DKEHPYGHGKFE+LGALGISSMLL TAGGIAWHA ++L 
Sbjct: 116 LSDIVLSGVALLSYKAAKAPRDKEHPYGHGKFESLGALGISSMLLVTAGGIAWHAFDVLQ 175

Query: 178 GLLSAAPEIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRA 237
           G++S+AP+I+   + H + H+H   GHHHGID++HPILAL++   +I +KEGLYWITKRA
Sbjct: 176 GVMSSAPDIIGN-VSHAH-HSHGSSGHHHGIDLEHPILALSVTAFAISVKEGLYWITKRA 235

Query: 238 GEKQGSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETG 297
           GEK+GSGLMKANAWHHR+DAISSVVAL+GVGGSILGV +LDPLAGLVVSGMILKAG+ TG
Sbjct: 236 GEKEGSGLMKANAWHHRSDAISSVVALLGVGGSILGVPYLDPLAGLVVSGMILKAGVHTG 295

Query: 298 YQSILELVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVS 357
           Y+S+LELVDAA+    + P+++TIL V+GVKGCHRLRGR+AG+SLYLDVHIEV PFLSVS
Sbjct: 296 YESVLELVDAAVDPSLLQPIKETILQVDGVKGCHRLRGRKAGTSLYLDVHIEVYPFLSVS 355

Query: 358 AAHCIGENVRQKIHTSHPEVSEVFIHI 382
           AAH IGE VR +I  SH +V+EVFIHI
Sbjct: 356 AAHDIGETVRHQIQKSHNQVAEVFIHI 378

BLAST of Lag0002518 vs. ExPASy Swiss-Prot
Match: Q2W8L4 (Magnetosome protein MamB OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=mamB PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.6e-30
Identity = 83/298 (27.85%), Postives = 142/298 (47.65%), Query Frame = 0

Query: 87  ADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAGKAPKDKEHPYGHGKF 146
           ADI +   K V G +SGS A++AD+ HS +DVV SG+   S K    P D+ +P+G+G  
Sbjct: 20  ADICMTLFKGVLGLMSGSVALVADSLHSGADVVASGVTQLSLKISNKPADERYPFGYGNI 79

Query: 147 ETLGALGISSMLLATAGGIAWHASELLLGLLSAAPEIVNQPLGHDNLHNHSHGGHHHGID 206
           + + +  + S+LL  A  + + +   L+     AP                         
Sbjct: 80  QYISSSIVGSLLLIGASFLMYGSVMKLISGTYEAPS------------------------ 139

Query: 207 MDHPILALNMAIISICIKEGLYWITKRAGEKQGSGLMKANAWHHRADAISSVVALIGVGG 266
               I A   A +++ + E +Y      G +  S  + ANAW +R+DAISS   ++GV  
Sbjct: 140 ----IFAAVGASVTVIVNELMYRYQICVGNENNSPAIIANAWDNRSDAISSAAVMVGVIA 199

Query: 267 SILGVKFLDPLAGLVVSGMILKAGLETGYQSILELVDAAIPADQINPVRQTILHVEGVKG 326
           S++G    D +A + VS ++ + GLE    SI  L+D+++  + +    Q  +    V  
Sbjct: 200 SVIGFPIADTIAAIGVSALVGRIGLELIGTSIHGLMDSSVDTELLQTAWQVAMDTPMVHS 259

Query: 327 CHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIGENVRQKIHTSHPEVSEVFIHIDPS 385
            + LRGR  G  +  D+ + VDP L +  +  + E VR++I    P   ++ + + P+
Sbjct: 260 IYFLRGRHVGEDVQFDIRLRVDPNLRIKDSSMVAEAVRRRIQEEIPHARDIRLFVSPA 289

BLAST of Lag0002518 vs. ExPASy Swiss-Prot
Match: O59758 (Uncharacterized metal transporter C1020.03 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC1020.03 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 6.0e-30
Identity = 92/301 (30.56%), Postives = 164/301 (54.49%), Query Frame = 0

Query: 83  LGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAGKAPKDKEHPYG 142
           LGL ++IGLAA K + G    S+ ++ADAAH + D +   + L + K       +++P G
Sbjct: 79  LGLYSNIGLAAAKGIGGVALQSSILVADAAHQLGDTLSDLVTLATLKICSKKPTQKYPAG 138

Query: 143 HGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAAPEIVNQPLGHDNLHNHSHGGHH 202
            GK+ET+G   +S +L+A + GIA H+S      LS    I+    G +    H+H GH 
Sbjct: 139 FGKWETIGTFTVSGLLVAVSVGIA-HSS------LSRLYTILFPYAGSE----HTHIGHS 198

Query: 203 HG---IDMDHPILALNMAIISICIKEGLYWITKRAGEKQGSGLMKANAWHHRADAISSVV 262
           H    +  +HP +AL +   S+ +KE L+  T+   +K  S ++ ANAWHHRADA++ +V
Sbjct: 199 HNPSQLLFEHPFMALGLIFGSVVLKEWLFRKTRTVAQKTDSNILLANAWHHRADALTGMV 258

Query: 263 ALIGVGGS-ILGVKFLDPLAGLVVSGMILKAGLETGYQSILELVDAAIPADQINPVRQTI 322
           +L+ + G+  L   +LDP  G +VS ++  AG  +  ++ L+L+D A P++++  +  T 
Sbjct: 259 SLLALSGTYFLNAPWLDPFFGCLVSIVVFSAGFNSSKKAFLQLLDRA-PSEELR-IAVTD 318

Query: 323 LHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIGENVRQKIHTSHPEVSEVF 380
             ++G K  +++     G++  + V I V P  +   +  + + V + +  + P +S   
Sbjct: 319 ALLKGEKLPYKI-VTILGNAHAMHVIISVPPSFTSQQSSELAQKVEKTVLDAIPALSSCI 365

BLAST of Lag0002518 vs. ExPASy Swiss-Prot
Match: V6F510 (Magnetosome protein MamB OS=Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1) OX=431944 GN=mamB PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.3e-29
Identity = 81/298 (27.18%), Postives = 141/298 (47.32%), Query Frame = 0

Query: 87  ADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAGKAPKDKEHPYGHGKF 146
           ADI +   K + G +SGS A++AD+ HS +DVV SG+   S K    P D+ +P+G+G  
Sbjct: 20  ADICMTLFKGILGLMSGSVALVADSLHSGADVVASGVTQLSLKISNKPADERYPFGYGNI 79

Query: 147 ETLGALGISSMLLATAGGIAWHASELLLGLLSAAPEIVNQPLGHDNLHNHSHGGHHHGID 206
           + + +  + S+LL  A  + + +   L+     AP                         
Sbjct: 80  QYISSAIVGSLLLIGASFLMYGSVVKLISGTYEAPS------------------------ 139

Query: 207 MDHPILALNMAIISICIKEGLYWITKRAGEKQGSGLMKANAWHHRADAISSVVALIGVGG 266
               I A   A +++ + E +Y      G +  S  + ANAW +R+DAISS   ++GV  
Sbjct: 140 ----IFAALGASVTVIVNELMYRYQICVGNENNSPAIIANAWDNRSDAISSAAVMVGVIA 199

Query: 267 SILGVKFLDPLAGLVVSGMILKAGLETGYQSILELVDAAIPADQINPVRQTILHVEGVKG 326
           S++G    D +A + VS ++   GLE   +++  L+D+++  + +    Q       V  
Sbjct: 200 SVIGFPIADTIAAIGVSALVGHIGLELIGKAVHGLMDSSVDTELLQTAWQIATDTPLVHS 259

Query: 327 CHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIGENVRQKIHTSHPEVSEVFIHIDPS 385
            + LRGR  G  +  D+ + VDP L +  +  + E VRQ+I    P   ++ + + P+
Sbjct: 260 IYFLRGRHVGEDVQFDIRLRVDPNLRIKDSSMVAEAVRQRIQDEIPHARDIRLFVSPA 289

BLAST of Lag0002518 vs. ExPASy TrEMBL
Match: A0A6J1GQ72 (metal tolerance protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111456474 PE=4 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 2.9e-253
Identity = 450/498 (90.36%), Postives = 467/498 (93.78%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           MGYRFHRLNPILQSFYSRLS +TH+EFHAIPS QS  LHPQ TLLGIY DPKSKICRRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRLSLATHKEFHAIPSTQSFDLHPQATLLGIYGDPKSKICRRWH 60

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHSHRHDD HRFG++GENIFKLGLGADIGLA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH++LHNHSHGGHHHGIDMDHPILALNM I+SI IKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMDHPILALNMTILSISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TIL VEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQN-TDVPLNEKNIEAI 420
           ENVRQ+IHTSHPE SEVFIHIDPSISH P  LS+QQGGS  T +QN  D+ L EKNIEAI
Sbjct: 361 ENVRQRIHTSHPEASEVFIHIDPSISHVPLKLSDQQGGSAGTSNQNIDDIALEEKNIEAI 420

Query: 421 VSIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAAS 480
           VS II S F E M+VERITPHLLQGKILLQIEVSMPP+LLIRNAMEVAKQAE+E+L AAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMEVAKQAEIEVLMAAS 480

Query: 481 NIVHVSIQLRLGHQIPQL 498
           NIVHVSI LRLGHQIP L
Sbjct: 481 NIVHVSILLRLGHQIPHL 498

BLAST of Lag0002518 vs. ExPASy TrEMBL
Match: A0A6J1JQY4 (metal tolerance protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111488105 PE=4 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.2e-252
Identity = 448/498 (89.96%), Postives = 467/498 (93.78%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           MGYRFHRLNPILQSF+SRLSS++H+EFHAIPS QS  LHPQTTLLGIY DPKSKICRRWH
Sbjct: 1   MGYRFHRLNPILQSFHSRLSSASHKEFHAIPSTQSFDLHPQTTLLGIYGDPKSKICRRWH 60

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHSHRHDD HRFG++GENIFKLGLGADIGLA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH++LHNHSHGGHHHGIDMDHPILALNM IISI IKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMDHPILALNMTIISISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TIL VEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQN-TDVPLNEKNIEAI 420
           ENVRQ+IHTSHPE SEVFIHIDPSISH P   S+QQGG+  T +QN  D+ L EKNIEAI
Sbjct: 361 ENVRQQIHTSHPEASEVFIHIDPSISHVPLKFSDQQGGTAGTSNQNIDDIALEEKNIEAI 420

Query: 421 VSIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAAS 480
           VS II S F E M+VERITPHLLQ KILLQIEVSMPP+LLIRNAMEVAKQAE+E+ KAAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQSKILLQIEVSMPPDLLIRNAMEVAKQAEIEVSKAAS 480

Query: 481 NIVHVSIQLRLGHQIPQL 498
           NIVHVSI LRLGHQIP L
Sbjct: 481 NIVHVSILLRLGHQIPHL 498

BLAST of Lag0002518 vs. ExPASy TrEMBL
Match: A0A0A0K5Y9 (Metal tolerance protein 6 OS=Cucumis sativus OX=3659 GN=MTP6 PE=2 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.6e-251
Identity = 446/499 (89.38%), Postives = 466/499 (93.39%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           MGYRFHRLNPILQSFYSR+S  TH+EFH+IPS QS  LHPQ T LGIYDDPKSKICRRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRISPPTHKEFHSIPSFQSPSLHPQFTFLGIYDDPKSKICRRWH 60

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHSHRHDD HRFG++GENIFKLGLGADIGLA GKAVTGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAVTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDNLHN--HSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQ 240
           PEIVNQP GH++LHN  HSHG HHHGIDMDHPILALNM IISICIKEGLYWITKRAGEK+
Sbjct: 181 PEIVNQPFGHESLHNHSHSHGEHHHGIDMDHPILALNMTIISICIKEGLYWITKRAGEKR 240

Query: 241 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSI 300
           GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL+TG+QSI
Sbjct: 241 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLQTGHQSI 300

Query: 301 LELVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC 360
           LELVDAAIPADQI+P +QTIL VEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAH 
Sbjct: 301 LELVDAAIPADQIDPFKQTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHS 360

Query: 361 IGENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQNTDVPLNEKNIEA 420
           IGENVR +IHTSHPEVSEVFIHIDPSISHFPP LSNQQ  S  T +Q+TD PL E NIEA
Sbjct: 361 IGENVRHEIHTSHPEVSEVFIHIDPSISHFPPKLSNQQAASAGTSNQSTDFPLTE-NIEA 420

Query: 421 IVSIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAA 480
            VS I+QS FPE M+VERITPHLLQGKILLQIEVSMPP+LLIRNAM+VAK+AEMEILKA 
Sbjct: 421 TVSDIVQSKFPENMMVERITPHLLQGKILLQIEVSMPPDLLIRNAMDVAKRAEMEILKAD 480

Query: 481 SNIVHVSIQLRLGHQIPQL 498
           SNIVHVSIQLRLG QIPQL
Sbjct: 481 SNIVHVSIQLRLGQQIPQL 498

BLAST of Lag0002518 vs. ExPASy TrEMBL
Match: A0A1S3BRB4 (metal tolerance protein C1 OS=Cucumis melo OX=3656 GN=LOC103492849 PE=4 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.9e-249
Identity = 443/499 (88.78%), Postives = 465/499 (93.19%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           MGYRFHRLNP+LQSFYSR+S  T  EFH+IPS QSL LHPQ TLLGIYDDPKSKICRRWH
Sbjct: 1   MGYRFHRLNPMLQSFYSRISPPTRNEFHSIPSFQSLNLHPQITLLGIYDDPKSKICRRWH 60

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHSHRHDD HRFG++GENIFKLGLGADIGLA GKAVTGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAVTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SG+ALWSFKAGKAPKDKEHPYGHGKFETLGALGIS+MLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGVALWSFKAGKAPKDKEHPYGHGKFETLGALGISTMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDNLHN--HSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQ 240
           PEIVNQP G ++LHN  HSHG HHHGIDMDHPILALNM IISICIKEGLYWITKRAGEKQ
Sbjct: 181 PEIVNQPFGLESLHNHSHSHGEHHHGIDMDHPILALNMTIISICIKEGLYWITKRAGEKQ 240

Query: 241 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSI 300
           GSGLMKANAWHH ADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL+TG+QSI
Sbjct: 241 GSGLMKANAWHHCADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLQTGHQSI 300

Query: 301 LELVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC 360
           LELVDAAIPADQI+P +QTIL +EGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAH 
Sbjct: 301 LELVDAAIPADQIDPFKQTILQIEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHS 360

Query: 361 IGENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQNTDVPLNEKNIEA 420
           IGENVRQKIHTSHPEVSEVFIHIDPSISHFPP LSN+Q  ST T +Q+T+  L EKNIEA
Sbjct: 361 IGENVRQKIHTSHPEVSEVFIHIDPSISHFPPKLSNKQASSTGTSNQSTNFLLTEKNIEA 420

Query: 421 IVSIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAA 480
            VS II S FPE M+VERITPHLLQGKILLQIEVSMPP+LLIRNAM+VAK+AE EILKA 
Sbjct: 421 TVSDIILSKFPENMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMDVAKRAENEILKAD 480

Query: 481 SNIVHVSIQLRLGHQIPQL 498
           SNIVHVSIQLRLGHQIPQL
Sbjct: 481 SNIVHVSIQLRLGHQIPQL 499

BLAST of Lag0002518 vs. ExPASy TrEMBL
Match: A0A6J1DDD3 (metal tolerance protein C1 OS=Momordica charantia OX=3673 GN=LOC111019455 PE=4 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.3e-249
Identity = 438/497 (88.13%), Postives = 469/497 (94.37%), Query Frame = 0

Query: 1   MGYRFHRLNPILQSFYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWH 60
           M YRF+RLNPILQSFY+RLSS+THQEFH+IP IQSL L PQ +LLGIYDDPK++I RRWH
Sbjct: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60

Query: 61  LGHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGH+HRHDDHHRFGE+GE+IFKLGLGADI LAAGKAVTGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKA KAPKDKEHPYGHGKFETLGALGIS MLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGHD+LHNHSHGGHHHGIDMDHPILALNM ++SI IKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL +G+QSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300

Query: 301 LVDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI PVR+TIL VEGV GCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQNTDVPLNEKNIEAIV 420
           EN R+KIHTSHPEVSEVFIHIDPSISHF  +LS+ Q GS  TL+Q+TD  LNEK IE+++
Sbjct: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIESMI 420

Query: 421 SIIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAASN 480
           S IIQS FPEKM+VERITPHLLQGKILL+IEVSMPP+LL+RNAMEVA+ AEM+ILKAASN
Sbjct: 421 SRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAASN 480

Query: 481 IVHVSIQLRLGHQIPQL 498
           IVHVSIQLRLGH+IPQL
Sbjct: 481 IVHVSIQLRLGHRIPQL 497

BLAST of Lag0002518 vs. TAIR 10
Match: AT2G47830.1 (Cation efflux family protein )

HSP 1 Score: 553.5 bits (1425), Expect = 1.7e-157
Identity = 302/492 (61.38%), Postives = 356/492 (72.36%), Query Frame = 0

Query: 4   RFHRLNPILQSFYSRLSSST--HQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWHL 63
           RF  LNP      +R+  ST  +     +PS  S    P          P     RRWH 
Sbjct: 5   RFQILNP------TRICRSTIYYMSCRYLPS-SSSSSSPLRVSSSSSQKPSFDFSRRWHF 64

Query: 64  GHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVLS 123
           GH   H  + + GE+GE IF+LGL ADIGL+  KA+TGYL GSTAIIADAAHSVSDVVLS
Sbjct: 65  GHPDHHQQYQKPGEEGEKIFRLGLTADIGLSVAKALTGYLCGSTAIIADAAHSVSDVVLS 124

Query: 124 GIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAAP 183
           G+AL S++A   PKDKEHPYGHGKFETLGALGIS+MLLAT  GIAWHA +LL   LSAAP
Sbjct: 125 GVALVSYRAANVPKDKEHPYGHGKFETLGALGISAMLLATGSGIAWHALDLLSIALSAAP 184

Query: 184 EIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGSG 243
           E++             H GHHHGIDM+HPILAL + I SI IKEGLYWITKRAGEKQGSG
Sbjct: 185 EVI-------------HSGHHHGIDMNHPILALTVTIASISIKEGLYWITKRAGEKQGSG 244

Query: 244 LMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILEL 303
           LM ANAWHHR+DAISS+VAL+GVGGSILGV FLDPLAGLVVS MI+ AGL+TG+QSILEL
Sbjct: 245 LMMANAWHHRSDAISSLVALVGVGGSILGVNFLDPLAGLVVSTMIVNAGLKTGHQSILEL 304

Query: 304 VDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIGE 363
           VDAAIPA Q+ P+RQTIL VEGVKGCHRLRGRRAGSSLYLDVHI VDPF SVS AH +GE
Sbjct: 305 VDAAIPAQQLEPIRQTILQVEGVKGCHRLRGRRAGSSLYLDVHIVVDPFSSVSVAHEVGE 364

Query: 364 NVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQNTDVPLNEKNIEAIVS 423
            VR++I+ +HPEVSEVFIHIDP+   F  +  +      +++ + +++    K++EA VS
Sbjct: 365 YVRRQINLNHPEVSEVFIHIDPAFLQFSCSTKDH-----DSITKESNICQEIKHVEATVS 424

Query: 424 IIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAASNI 483
            I  S   EK+ ++RITPHLL  KILLQI V+MP  + I++ M  A+ AE EILKAA N+
Sbjct: 425 DIFSSQLSEKLTIKRITPHLLHSKILLQIVVAMPSTMSIQDVMIAAEHAEKEILKAAPNV 471

Query: 484 VHVSIQLRLGHQ 494
             VSIQL L  +
Sbjct: 485 ARVSIQLSLNSE 471

BLAST of Lag0002518 vs. TAIR 10
Match: AT2G47830.2 (Cation efflux family protein )

HSP 1 Score: 547.4 bits (1409), Expect = 1.2e-155
Identity = 301/492 (61.18%), Postives = 354/492 (71.95%), Query Frame = 0

Query: 4   RFHRLNPILQSFYSRLSSST--HQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWHL 63
           RF  LNP      +R+  ST  +     +PS  S    P          P     RRWH 
Sbjct: 5   RFQILNP------TRICRSTIYYMSCRYLPS-SSSSSSPLRVSSSSSQKPSFDFSRRWHF 64

Query: 64  GHSHRHDDHHRFGEKGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVLS 123
           GH   H  + + GE+GE IF+LGL ADIGL+  KA+TGYL GSTAIIADAAHSVSDVVLS
Sbjct: 65  GHPDHHQQYQKPGEEGEKIFRLGLTADIGLSVAKALTGYLCGSTAIIADAAHSVSDVVLS 124

Query: 124 GIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAAP 183
           G+AL S++A   PKDKEHPYGHGKFETLGALGIS+MLLAT  GIAWHA +LL   LSAAP
Sbjct: 125 GVALVSYRAANVPKDKEHPYGHGKFETLGALGISAMLLATGSGIAWHALDLLSIALSAAP 184

Query: 184 EIVNQPLGHDNLHNHSHGGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGSG 243
           E++             H GHHHGIDM+HPILAL + I SI IKEGLYWITKRAGEKQGSG
Sbjct: 185 EVI-------------HSGHHHGIDMNHPILALTVTIASISIKEGLYWITKRAGEKQGSG 244

Query: 244 LMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILEL 303
           LM ANAWHHR+DAISS+VAL+GVGGSILGV FLDPLAGLVVS MI+ AGL+TG+QSILEL
Sbjct: 245 LMMANAWHHRSDAISSLVALVGVGGSILGVNFLDPLAGLVVSTMIVNAGLKTGHQSILEL 304

Query: 304 VDAAIPADQINPVRQTILHVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIGE 363
           VDAAIPA Q+ P+RQTIL VEGVKGCHRLRGRRAGSSLYLDVHI VDPF SVS AH +GE
Sbjct: 305 VDAAIPAQQLEPIRQTILQVEGVKGCHRLRGRRAGSSLYLDVHIVVDPFSSVSVAHEVGE 364

Query: 364 NVRQKIHTSHPEVSEVFIHIDPSISHFPPNLSNQQGGSTETLDQNTDVPLNEKNIEAIVS 423
            VR++I+ +HPEVSEVFIHIDP+   F  +  +      +++ + +++    K++EA VS
Sbjct: 365 YVRRQINLNHPEVSEVFIHIDPAFLQFSCSTKDH-----DSITKESNICQEIKHVEATVS 424

Query: 424 IIIQSNFPEKMLVERITPHLLQGKILLQIEVSMPPELLIRNAMEVAKQAEMEILKAASNI 483
            I  S   EK+ ++RITPHLL  KILLQI V+MP  +   + M  A+ AE EILKAA N+
Sbjct: 425 DIFSSQLSEKLTIKRITPHLLHSKILLQIVVAMPSTM---DVMIAAEHAEKEILKAAPNV 468

Query: 484 VHVSIQLRLGHQ 494
             VSIQL L  +
Sbjct: 485 ARVSIQLSLNSE 468

BLAST of Lag0002518 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 63.9 bits (154), Expect = 4.1e-10
Identity = 89/314 (28.34%), Postives = 138/314 (43.95%), Query Frame = 0

Query: 95  KAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAGKAPKDKEHPYGHGKFETLGALGI 154
           + V G  + S AI+ DAAH +SDV    I+L+S  A     + +  YG  + E LGAL  
Sbjct: 88  EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVS 147

Query: 155 SSMLLATAGGIAWHA-----------SELLLGLLSAAPEIVNQP----LGHDNLHNHSH- 214
             M+   AG + + A              L+  +SA   +VN      LGHD+ H H H 
Sbjct: 148 IQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHS 207

Query: 215 --GGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGSGLMKANAWHHRADAIS 274
              GH H  D  H I A      S   +  L  +     +KQ +  ++    H   D+I 
Sbjct: 208 HDNGHGHSHDHGHGIAATEHHHDSGHDESQLSDVLIEQ-KKQRNVNIQGAYLHVLGDSIQ 267

Query: 275 SVVALIGVGGSILGV----KFLDPLAGLVVSGMILKAGLETG-YQSILELVDAAIPADQI 334
           SV  +I  GG+I+      K LD +  LV S ++L  G   G  ++ILE++  + P  +I
Sbjct: 268 SVGVMI--GGAIIWYKPEWKILDLICTLVFSVIVL--GTTIGMLRNILEVLMESTPR-EI 327

Query: 335 NP--VRQTILHVEGVKGCHRLR-GRRAGSSLYLDVHIEVDPFLSVSAAHCIGENVRQKIH 383
           +P  + + +  +E V   H L         L L  H+++ P    + A  + + +   I 
Sbjct: 328 DPTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRP---EAEADMVLDKIIDYIK 387

BLAST of Lag0002518 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 62.8 bits (151), Expect = 9.2e-10
Identity = 103/396 (26.01%), Postives = 166/396 (41.92%), Query Frame = 0

Query: 15  FYSRLSSSTHQEFHAIPSIQSLKLHPQTTLLGIYDDPKSKICRRWHLGHSHRHDDHHRFG 74
           F+ ++    H+  H +     +    +T+L+GI      K C     G S          
Sbjct: 51  FFFQMKDHIHEHDHMVQICGEVS-SGETSLVGI-----KKTCGEAPCGFSDAKTSSIEAQ 110

Query: 75  EKGENIFKLGLGADIG--LAAGKAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAGK 134
           E+  ++ KL +   +       + V G  + S AI+ DAAH +SDV    I+L+S  A  
Sbjct: 111 ERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASG 170

Query: 135 APKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHA-----------SELLLGLLSAAP 194
              + +  YG  + E LGAL    M+   AG + + A              L+  +SA  
Sbjct: 171 WKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVG 230

Query: 195 EIVNQP----LGHDNLHNHSH---GGHHHGIDMDHPILALNMAIISICIKEGLYWITKRA 254
            +VN      LGHD+ H H H    GH H  D  H I A      S   +  L  +    
Sbjct: 231 LLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATEHHHDSGHDESQLSDVLIEQ 290

Query: 255 GEKQGSGLMKANAWHHRADAISSVVALIGVGGSILGV----KFLDPLAGLVVSGMILKAG 314
            +KQ +  ++    H   D+I SV  +I  GG+I+      K LD +  LV S ++L  G
Sbjct: 291 -KKQRNVNIQGAYLHVLGDSIQSVGVMI--GGAIIWYKPEWKILDLICTLVFSVIVL--G 350

Query: 315 LETG-YQSILELVDAAIPADQINP--VRQTILHVEGVKGCHRLR-GRRAGSSLYLDVHIE 374
              G  ++ILE++  + P  +I+P  + + +  +E V   H L         L L  H++
Sbjct: 351 TTIGMLRNILEVLMESTPR-EIDPTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVK 410

Query: 375 VDPFLSVSAAHCIGENVRQKIHTSHPEVSEVFIHID 383
           + P    + A  + + +   I   H  +S V I I+
Sbjct: 411 IRP---EAEADMVLDKIIDYIKREH-NISHVTIQIE 430

BLAST of Lag0002518 vs. TAIR 10
Match: AT2G29410.1 (metal tolerance protein B1 )

HSP 1 Score: 56.6 bits (135), Expect = 6.6e-08
Identity = 68/256 (26.56%), Postives = 112/256 (43.75%), Query Frame = 0

Query: 97  VTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISS 156
           V G+ + S A++ DAAH +SDV    ++L + K      +  + +G  + E L A  +S 
Sbjct: 74  VGGFKANSLAVMTDAAHLLSDVAGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAF-LSV 133

Query: 157 MLLATAGGIAWHASELLLGLLSAAPEIVNQP------------------LGHDNLHNHSH 216
            L+    G+  H  E +  LLS + E+  +                   LGH++ H+H H
Sbjct: 134 QLIWLVSGVIIH--EAIQRLLSRSREVNGEIMFGISAFGFFMNLVMVLWLGHNHSHHH-H 193

Query: 217 GGHHHGIDMDHPILALNMAIISICIKEGLYWITKRAGEKQGSGLMKANA----WHHRADA 276
             HHH  +  H     +  +++   +E    +    GEK  S  M  N      H  AD 
Sbjct: 194 DHHHHHHNHKHQHQHHHKEVVA---EEEEEEMNPLKGEKSSSKEMNINIQGAYLHAMADM 253

Query: 277 ISSVVALIGVGGSILGVK----FLDPLAGLVVSGMILKAGLETGYQSILELVDAAIPAD- 326
           I S+  +I  GG I+ VK     +D +  LV S   L A L    ++I  ++   +P D 
Sbjct: 254 IQSLGVMI--GGGIIWVKPKWVLVDLICTLVFSAFALAATLPI-LKNIFGILMERVPRDM 313