Lag0002428 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002428
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAdenylate isopentenyltransferase 3
Locationchr4: 42786446 .. 42787438 (+)
RNA-Seq ExpressionLag0002428
SyntenyLag0002428
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGTGTCCACCATGTTCATGAGCAAACAGATCACTCAACAACCATTGATAAATCTTCCCCCAGGAAGACTAAACACCAATCCTTTCTTCAACCTCAGGAAGCTGCAAAAAGAGAAGGTGGTGATCGTGATCGGCGCCACCGGTACCGGAAAATCTCGGCTTTCGATCGACATCGCCACCCGATTCCCGTCGGAAGTCATCAACTCCGACAAAATGCAAGTCTACGACGGCCTCGACATAGTAACAAACAAGGTCACCAAGCAAGAACAACGCGGCGTGCCCCATCACTTGCTCGGAATCATCAACCCCTTCTCGGACTTCTCGGCGACCAGCTTCTCCGATATGGCCACGCTCTCCTTGGACGCCATCTCCGGCCGCGACCGCCTCCCGGTCATCGCCGGTGGCTCGAATTCTTACATCGAAACCTTAATCGACTCGAACTTTCGATCGAAATACGAATGTTGCTTCCTTTGGGTGGATGTGTCAATGCCTGTATTGCACTCCTTTGTATCCAAACGAGTAGACAAGATGGTAGCGAATGGAATGGTGGATGAGGCTGAAAAATTCTTCGATCCTGTCGGAGATTATTCTCGAGGGATCAAAAGGGCAATTGGGGTTCCTGAATTTGACTCGTATTTTCGATACAAATCATTGTTAGATGAGGAAACTCAAGATGAACTTTTGCAAGAAGCTATATCAAAAGTGAAGGAGCACACATGCAAGCTAGCATTGAGACAATTGGGGAAGATCCAACGGCTCAGAAATGTCAACGGGTGGAACATACATAGGTTGGATGCTACAGAGGCATTTACAAAGCAAGGGAAAGAAGCAGAGGAAGCATGGAACAAGCTCGTCATGGCGCCGAGCACCACCATAATCGACCAGTTTCTTCACCCCAATGTCATCACTGCCAAACTCCCCACGAATTTAGCATCCCTCATCGGCCTGTCGCGGAACAGAAGTGCAGCCATTGCAACTGCAACTCCTTAA

mRNA sequence

ATGAATGTGTCCACCATGTTCATGAGCAAACAGATCACTCAACAACCATTGATAAATCTTCCCCCAGGAAGACTAAACACCAATCCTTTCTTCAACCTCAGGAAGCTGCAAAAAGAGAAGGTGGTGATCGTGATCGGCGCCACCGGTACCGGAAAATCTCGGCTTTCGATCGACATCGCCACCCGATTCCCGTCGGAAGTCATCAACTCCGACAAAATGCAAGTCTACGACGGCCTCGACATAGTAACAAACAAGGTCACCAAGCAAGAACAACGCGGCGTGCCCCATCACTTGCTCGGAATCATCAACCCCTTCTCGGACTTCTCGGCGACCAGCTTCTCCGATATGGCCACGCTCTCCTTGGACGCCATCTCCGGCCGCGACCGCCTCCCGGTCATCGCCGGTGGCTCGAATTCTTACATCGAAACCTTAATCGACTCGAACTTTCGATCGAAATACGAATGTTGCTTCCTTTGGGTGGATGTGTCAATGCCTGTATTGCACTCCTTTGTATCCAAACGAGTAGACAAGATGGTAGCGAATGGAATGGTGGATGAGGCTGAAAAATTCTTCGATCCTGTCGGAGATTATTCTCGAGGGATCAAAAGGGCAATTGGGGTTCCTGAATTTGACTCGTATTTTCGATACAAATCATTGTTAGATGAGGAAACTCAAGATGAACTTTTGCAAGAAGCTATATCAAAAGTGAAGGAGCACACATGCAAGCTAGCATTGAGACAATTGGGGAAGATCCAACGGCTCAGAAATGTCAACGGGTGGAACATACATAGGTTGGATGCTACAGAGGCATTTACAAAGCAAGGGAAAGAAGCAGAGGAAGCATGGAACAAGCTCGTCATGGCGCCGAGCACCACCATAATCGACCAGTTTCTTCACCCCAATGTCATCACTGCCAAACTCCCCACGAATTTAGCATCCCTCATCGGCCTGTCGCGGAACAGAAGTGCAGCCATTGCAACTGCAACTCCTTAA

Coding sequence (CDS)

ATGAATGTGTCCACCATGTTCATGAGCAAACAGATCACTCAACAACCATTGATAAATCTTCCCCCAGGAAGACTAAACACCAATCCTTTCTTCAACCTCAGGAAGCTGCAAAAAGAGAAGGTGGTGATCGTGATCGGCGCCACCGGTACCGGAAAATCTCGGCTTTCGATCGACATCGCCACCCGATTCCCGTCGGAAGTCATCAACTCCGACAAAATGCAAGTCTACGACGGCCTCGACATAGTAACAAACAAGGTCACCAAGCAAGAACAACGCGGCGTGCCCCATCACTTGCTCGGAATCATCAACCCCTTCTCGGACTTCTCGGCGACCAGCTTCTCCGATATGGCCACGCTCTCCTTGGACGCCATCTCCGGCCGCGACCGCCTCCCGGTCATCGCCGGTGGCTCGAATTCTTACATCGAAACCTTAATCGACTCGAACTTTCGATCGAAATACGAATGTTGCTTCCTTTGGGTGGATGTGTCAATGCCTGTATTGCACTCCTTTGTATCCAAACGAGTAGACAAGATGGTAGCGAATGGAATGGTGGATGAGGCTGAAAAATTCTTCGATCCTGTCGGAGATTATTCTCGAGGGATCAAAAGGGCAATTGGGGTTCCTGAATTTGACTCGTATTTTCGATACAAATCATTGTTAGATGAGGAAACTCAAGATGAACTTTTGCAAGAAGCTATATCAAAAGTGAAGGAGCACACATGCAAGCTAGCATTGAGACAATTGGGGAAGATCCAACGGCTCAGAAATGTCAACGGGTGGAACATACATAGGTTGGATGCTACAGAGGCATTTACAAAGCAAGGGAAAGAAGCAGAGGAAGCATGGAACAAGCTCGTCATGGCGCCGAGCACCACCATAATCGACCAGTTTCTTCACCCCAATGTCATCACTGCCAAACTCCCCACGAATTTAGCATCCCTCATCGGCCTGTCGCGGAACAGAAGTGCAGCCATTGCAACTGCAACTCCTTAA

Protein sequence

MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLSLDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHTCKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHPNVITAKLPTNLASLIGLSRNRSAAIATATP
Homology
BLAST of Lag0002428 vs. NCBI nr
Match: XP_038899604.1 (adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 592.0 bits (1525), Expect = 3.1e-165
Identity = 294/330 (89.09%), Postives = 309/330 (93.64%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           M+VSTMFMSKQITQQPLINLPPGRLNTNPFFNLRK QKEKVVIVIGATGTGKSRLS+DIA
Sbjct: 1   MDVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKPQKEKVVIVIGATGTGKSRLSVDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           TRFPSEVINSDKMQVY GLDIVTNK+TKQEQRGVPHHLLGIINPFSDFSAT+FSDMA LS
Sbjct: 61  TRFPSEVINSDKMQVYRGLDIVTNKITKQEQRGVPHHLLGIINPFSDFSATNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LDAI+  DRLP+IAGGSNSYIE LIDS FRSKYECCFLWVDVSM VL SFVS+RVDKMVA
Sbjct: 121 LDAITSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMVDEAEKFFDP  DYSRGIKRAIGVPEFDSYFRYK LLDEE QD+LL+E+ISKVKEHT
Sbjct: 181 NGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKPLLDEEAQDKLLKESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLALRQLGKIQRLRN+ GWNIHRLDATEAFTKQGKEAEEAWNKLV  PSTT+IDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTVPSTTLIDQFLHP 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           NV+  K PTNLASLIG+SR RSAA+A ATP
Sbjct: 301 NVVATKFPTNLASLIGVSRKRSAAMAAATP 330

BLAST of Lag0002428 vs. NCBI nr
Match: KAG7025533.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 589.0 bits (1517), Expect = 2.6e-164
Identity = 290/330 (87.88%), Postives = 312/330 (94.55%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN+RKL+KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           TRFPSEVINSDKMQVYDGLDI+TNKVTKQEQRGVPHHLLGII+PFSDFSAT+FSDMA LS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LD I GRDRLP+IAGGSNSY+E LIDSNF+SKYECCFLWVDVSMPVLHSFVSKRVDKMVA
Sbjct: 121 LDTIFGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMVDEAE+FFDP GDYSRGIKRAIGVPEFDSYFRY+SLLD+ETQ++LLQEAISKVK++T
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLA RQ+GKIQRLRN+ GW IHRLDATEAF KQGKEAEE WNKLV  PSTTIIDQFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           NVIT K+PTNLA+L+ LSR RSAAIA ATP
Sbjct: 301 NVITTKVPTNLATLLDLSRKRSAAIAAATP 330

BLAST of Lag0002428 vs. NCBI nr
Match: XP_023514910.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.8 bits (1514), Expect = 5.8e-164
Identity = 289/330 (87.58%), Postives = 312/330 (94.55%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN+RKL+KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           TRFPSEVINSDKMQVYDGLDI+TNKVTKQEQRGVPHHLLGII+PFSDFSAT+FSDMA LS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LD I GRDRLP+IAGGSNSY+E LIDSNF+SKYECCFLWVDVSMPVLHSFVSKRVDKMVA
Sbjct: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMVDEAE+FFDP GDYSRGIKRAIGVPEFDSYFRY+SLL++ETQ++LLQEAISKVK++T
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLA RQ+GKIQRLRN+ GW IHRLDATEAF KQGKEAEE WNKLV  PSTTIIDQFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           NVIT K+PTNLA+L+GL R RSAAIA ATP
Sbjct: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330

BLAST of Lag0002428 vs. NCBI nr
Match: XP_022960175.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 585.1 bits (1507), Expect = 3.8e-163
Identity = 290/330 (87.88%), Postives = 311/330 (94.24%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN+RKL+KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGII+PFSDFSAT+FSDMA LS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           L  I GRDRLP+IAGGSNSY+E LIDSNF+SKYECCFLWVDVSMPVLHSFVSKRVDKMVA
Sbjct: 121 LVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMVDEAE+FFDP GDYSRGIKRAIGVPEFDSYFRY+SLLD+ETQ++LLQEAISKVK++T
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLA RQ+GKIQRLRN+ GW IHRLDATEAF KQGKEAEE WNKLV  PSTTIIDQFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           NVIT K+PTNLA+L+ LSR RSAAIA ATP
Sbjct: 301 NVITTKVPTNLATLLDLSRMRSAAIAAATP 330

BLAST of Lag0002428 vs. NCBI nr
Match: XP_023004596.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 582.8 bits (1501), Expect = 1.9e-162
Identity = 287/330 (86.97%), Postives = 311/330 (94.24%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN+RKL+KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           T+FPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGII+PFSDFSAT+FSDMA LS
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LD I GRDRLP+IAGGSNSYIE LIDSNF+SKYECCFLWVDVSMP+LHSFVSKRVDKMVA
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMV+EA++FFDP GDYSRGIKRAIGVPEFDSYFRY+SLLD+ETQ++LLQEAISKVK++T
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLA RQ+GKIQRLRN+ GW IHRLDATEAF KQGKEAEE WNKLV  PSTTII+QFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           NVIT  +PTNLASL+GL R RSAAIA ATP
Sbjct: 301 NVITTNVPTNLASLLGLPRKRSAAIAAATP 330

BLAST of Lag0002428 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.2e-87
Identity = 158/304 (51.97%), Postives = 217/304 (71.38%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N         K+KVV+++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           TRF +E+INSDK+QV+ GLDIVTNK+T +E  GVPHHLLG++ P +D +A ++  MA LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDAISGRDRLPVIAGGSNSYIETLID---SNFRSKYECCFLWVDVSMPVLHSFVSKRVDK 180
           ++++  R +LP+I GGSNSY+E L+D   + FRS+Y+CCFLWVDV++PVLH FVS+RVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVANGMVDEAEKFFD-PVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKV 240
           MV +GMV+E  +FFD    DYSRGIK+AIG PEFD +FR +  L+ E ++ELL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGK--EAEEAWNKLVMAPSTTII 299
           K +T +LA RQ  KI+RLR V  W+I R+DAT  FTK+    +A  AW +LV  PST  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

BLAST of Lag0002428 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 317.8 bits (813), Expect = 1.5e-85
Identity = 165/289 (57.09%), Postives = 210/289 (72.66%), Query Frame = 0

Query: 15  QPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQ 74
           QPL     G +   PFF     +K+KVV V+GATGTGKSRL+ID+ATRFP+E++NSDK+Q
Sbjct: 13  QPLSLNFQGNMVDVPFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQ 72

Query: 75  VYDGLDIVTNKVTKQEQRGVPHHLLGII-NPFSDFSATSFSDMATLSLDAISGRDRLPVI 134
           VY GLDIVTNKVT +E  GVPHHLLG + + + DF+A  F   A  ++++I  RDR+P+I
Sbjct: 73  VYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPII 132

Query: 135 AGGSNSYIETLIDS--NFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEKFF 194
           AGGSNSYIE L++   +FR +Y CCFLWVDVS PVLHSFVS+RVDKMV  G+VDE  + F
Sbjct: 133 AGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIF 192

Query: 195 DP-VGDYSRGIKRAIGVPEFDSYFRYKSL-LDEETQDELLQEAISKVKEHTCKLALRQLG 254
           DP   DYS GI+RAIGVPE D + R +      ET + LL+ AI K+KE+TC LA RQL 
Sbjct: 193 DPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQ 252

Query: 255 KIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFL 299
           KIQRL     WN+HR+DATE F ++G+EA+EAW+  V  PS   +++FL
Sbjct: 253 KIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of Lag0002428 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 287.0 bits (733), Expect = 2.8e-76
Identity = 142/283 (50.18%), Postives = 205/283 (72.44%), Query Frame = 0

Query: 24  RLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKVTKQEQRGVPHHLLGII-NPFSDFSATSFSDMATLSLDAISGRDRLPVIAGGSNSYIE 143
           NKVT +E RGVPHHLLG+  +   + +AT +S +A+ ++  +S  ++LP++AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 TLIDSN---FRSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEKFFDPVGDYSR 203
            L++ +     + Y+CCF+WVDVS+PVL+SFVSKRVD+M+  G+++E  + F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHTCKLALRQLGKIQRLRNVNG 263
           GI+RAIGVPE   Y R +SL+D  T+ ++L  A+  +K++T  LA RQL KIQRL     
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFTKQG-KEAEEAWNKLVMAPSTTIIDQFLHPN 302
            ++HR+DATE F K+  +E +EAW  LV  PS  I+D+F + N
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

BLAST of Lag0002428 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.8e-65
Identity = 137/317 (43.22%), Postives = 191/317 (60.25%), Query Frame = 0

Query: 21  PPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLD 80
           P     TN     ++ +KEK+++++GATGTGKSRLSID+A  FP EVINSDKMQVY GLD
Sbjct: 12  PTTTTTTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLD 71

Query: 81  IVTNKVTKQEQRGVPHHLLGIINPF-SDFSATSFSDMATLSLDAISGRDRLPVIAGGSNS 140
           I TNK++  ++ GVPHHLLG ++P   + +   F  +A  ++  I+GR +LPV+ GGSNS
Sbjct: 72  ITTNKISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNS 131

Query: 141 YIETLIDSNFRS------------------KYECCFLWVDVSMPVLHSFVSKRVDKMVAN 200
           +I  L+   F S                  +Y+CCFLWVDVS+ VL  +++KRVD M+  
Sbjct: 132 FIHALLVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLEL 191

Query: 201 GMVDEAEKFFDPVGD-------YSRGIKRAIGVPEFDSYFRYKSLLDEETQDE------- 260
           GM DE  +F+ P  +          G+++AIGVPEFD YF      D E +D        
Sbjct: 192 GMFDELAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRR 251

Query: 261 -LLQEAISKVKEHTCKLALRQLGKIQRLRNVNGWNIHRLDATEAF-----TKQGKEAEEA 299
              +EA+  +KE+TC LA RQ+GKI RL+   GW++ RLDATE+F     +  G++  E 
Sbjct: 252 GAFEEAVRAIKENTCHLAKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEI 311

BLAST of Lag0002428 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 244.6 bits (623), Expect = 1.6e-63
Identity = 136/336 (40.48%), Postives = 203/336 (60.42%), Query Frame = 0

Query: 4   STMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQ-------KEKVVIVIGATGTGKSRLS 63
           S  F S   +   L++    R    P  +  +++       K+KVV+++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINP-FSDFSATSFSD 123
           +D+ATRFPSE+INSDK+QVY+GL+I TN++T Q++RGVPHHLLG+INP   + +A  F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 MATLSLDAISGRDRLPVIAGGSNSYIETLIDSNFRSK----------------YECCFLW 183
            A+  +  I+ R ++P+IAGGSNS++  L+   F  K                YECCF+W
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSMPVLHSFVSKRVDKMVANGMVDEAEKFFDPV--GDYSR-GIKRAIGVPEFDSYFR- 243
           VDVS  VL+ ++ +RVD+M+ +GM +E  +F+DPV  G  +R GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YKSLLDEETQDELLQEAISKVKEHTCKLALRQLGKIQRLRNVNGWNIHRLDATEAF 302
                K +  +  +     +A+  +K +T  LA RQ+ KI+ L++  GW I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDA-GWEIERVDATASF 322

BLAST of Lag0002428 vs. ExPASy TrEMBL
Match: A0A6J1H8C9 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460993 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.8e-163
Identity = 290/330 (87.88%), Postives = 311/330 (94.24%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN+RKL+KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGII+PFSDFSAT+FSDMA LS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           L  I GRDRLP+IAGGSNSY+E LIDSNF+SKYECCFLWVDVSMPVLHSFVSKRVDKMVA
Sbjct: 121 LVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMVDEAE+FFDP GDYSRGIKRAIGVPEFDSYFRY+SLLD+ETQ++LLQEAISKVK++T
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLA RQ+GKIQRLRN+ GW IHRLDATEAF KQGKEAEE WNKLV  PSTTIIDQFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           NVIT K+PTNLA+L+ LSR RSAAIA ATP
Sbjct: 301 NVITTKVPTNLATLLDLSRMRSAAIAAATP 330

BLAST of Lag0002428 vs. ExPASy TrEMBL
Match: A0A6J1KSJ2 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497849 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 9.1e-163
Identity = 287/330 (86.97%), Postives = 311/330 (94.24%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN+RKL+KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           T+FPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGII+PFSDFSAT+FSDMA LS
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LD I GRDRLP+IAGGSNSYIE LIDSNF+SKYECCFLWVDVSMP+LHSFVSKRVDKMVA
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMV+EA++FFDP GDYSRGIKRAIGVPEFDSYFRY+SLLD+ETQ++LLQEAISKVK++T
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLA RQ+GKIQRLRN+ GW IHRLDATEAF KQGKEAEE WNKLV  PSTTII+QFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           NVIT  +PTNLASL+GL R RSAAIA ATP
Sbjct: 301 NVITTNVPTNLASLLGLPRKRSAAIAAATP 330

BLAST of Lag0002428 vs. ExPASy TrEMBL
Match: A0A5D3DBM7 (Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00080 PE=4 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 1.0e-158
Identity = 284/330 (86.06%), Postives = 302/330 (91.52%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN FFNLRKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFFNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           TRFPSEVINSDKMQVY GLDIVTNK+TKQEQ GVPHHLLGIINPF DFSA++FSDMA LS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LDAIS  DRLP+IAGGSNSYIE LIDSNFR KYECCFLWVDVSM VL SFVS+RVDKMVA
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMVDEAEKFFDP  DYSRGIKRAIGVPEFDSYFR +SLLDEE Q++LL+E+I+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLAL+QLGKIQRLRN+ GWNIHRLDATEAFTKQGKEAEEAWNKLV  PSTTIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           N +  K PTNLASLIG+ R RSAA+A ATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

BLAST of Lag0002428 vs. ExPASy TrEMBL
Match: A0A1S3BRC2 (adenylate isopentenyltransferase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492689 PE=4 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.6e-157
Identity = 282/330 (85.45%), Postives = 301/330 (91.21%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN F NLRKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFSNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           T+FPSEVINSDKMQVY GLDIVTNK+TKQEQ GVPHHLLGIINPF DFSA++FSDMA LS
Sbjct: 61  TQFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LDAIS  DRLP+IAGGSNSYIE LIDSNFR KYECCFLWVDVSM VL SFVS+RVDKMVA
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMVDEAEKFFDP  DYSRGIKRAIGVPEFDSYFR +SLLDEE Q++LL+E+I+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLAL+QLGKIQRLRN+ GWNIHRLDATEAFTKQGKEAEEAWNKLV  PSTTIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           N +  K PTNLASLIG+ R RSAA+A ATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

BLAST of Lag0002428 vs. ExPASy TrEMBL
Match: A0A0A0K919 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.4e-157
Identity = 283/330 (85.76%), Postives = 300/330 (90.91%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           MNVS+MFMSK  TQQPLINLPPGRL TNPF  LRKLQKEKVVIVIG TGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSKITTQQPLINLPPGRLTTNPFSTLRKLQKEKVVIVIGPTGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           TRFPSEVINSDKMQVY GLDIVTNK+TKQEQ GVPHHLLGIINPF DFSA++FSDMA LS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISGRDRLPVIAGGSNSYIETLIDSNFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LDAIS  DRLP+IAGGSNSYIE LIDSNFR +YECCFLWVDVSM VL SFVS+RVDKMVA
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAEKFFDPVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHT 240
           NGMVDEAEKFFDP  DYSRGIKRAIGVPEFDSYFRY+SLLDEE Q++LL+E+I+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFLHP 300
           CKLALRQLGKIQRLRNV GWNIHRLDATEAFTKQGKEA+EAWNK V  PSTTIIDQFLH 
Sbjct: 241 CKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQGKEADEAWNKHVTIPSTTIIDQFLHQ 300

Query: 301 NVITAKLPTNLASLIGLSRNRSAAIATATP 331
           NVI  K PTNLASLIG+ R +SAA+A ATP
Sbjct: 301 NVIATKFPTNLASLIGVPRKKSAAMAAATP 330

BLAST of Lag0002428 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 324.7 bits (831), Expect = 8.6e-89
Identity = 158/304 (51.97%), Postives = 217/304 (71.38%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N         K+KVV+++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINPFSDFSATSFSDMATLS 120
           TRF +E+INSDK+QV+ GLDIVTNK+T +E  GVPHHLLG++ P +D +A ++  MA LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDAISGRDRLPVIAGGSNSYIETLID---SNFRSKYECCFLWVDVSMPVLHSFVSKRVDK 180
           ++++  R +LP+I GGSNSY+E L+D   + FRS+Y+CCFLWVDV++PVLH FVS+RVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVANGMVDEAEKFFD-PVGDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKV 240
           MV +GMV+E  +FFD    DYSRGIK+AIG PEFD +FR +  L+ E ++ELL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALRQLGKIQRLRNVNGWNIHRLDATEAFTKQGK--EAEEAWNKLVMAPSTTII 299
           K +T +LA RQ  KI+RLR V  W+I R+DAT  FTK+    +A  AW +LV  PST  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

BLAST of Lag0002428 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 317.8 bits (813), Expect = 1.0e-86
Identity = 165/289 (57.09%), Postives = 210/289 (72.66%), Query Frame = 0

Query: 15  QPLINLPPGRLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQ 74
           QPL     G +   PFF     +K+KVV V+GATGTGKSRL+ID+ATRFP+E++NSDK+Q
Sbjct: 13  QPLSLNFQGNMVDVPFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQ 72

Query: 75  VYDGLDIVTNKVTKQEQRGVPHHLLGII-NPFSDFSATSFSDMATLSLDAISGRDRLPVI 134
           VY GLDIVTNKVT +E  GVPHHLLG + + + DF+A  F   A  ++++I  RDR+P+I
Sbjct: 73  VYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPII 132

Query: 135 AGGSNSYIETLIDS--NFRSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEKFF 194
           AGGSNSYIE L++   +FR +Y CCFLWVDVS PVLHSFVS+RVDKMV  G+VDE  + F
Sbjct: 133 AGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIF 192

Query: 195 DP-VGDYSRGIKRAIGVPEFDSYFRYKSL-LDEETQDELLQEAISKVKEHTCKLALRQLG 254
           DP   DYS GI+RAIGVPE D + R +      ET + LL+ AI K+KE+TC LA RQL 
Sbjct: 193 DPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQ 252

Query: 255 KIQRLRNVNGWNIHRLDATEAFTKQGKEAEEAWNKLVMAPSTTIIDQFL 299
           KIQRL     WN+HR+DATE F ++G+EA+EAW+  V  PS   +++FL
Sbjct: 253 KIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of Lag0002428 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 142/283 (50.18%), Postives = 205/283 (72.44%), Query Frame = 0

Query: 24  RLNTNPFFNLRKLQKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKVTKQEQRGVPHHLLGII-NPFSDFSATSFSDMATLSLDAISGRDRLPVIAGGSNSYIE 143
           NKVT +E RGVPHHLLG+  +   + +AT +S +A+ ++  +S  ++LP++AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 TLIDSN---FRSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEKFFDPVGDYSR 203
            L++ +     + Y+CCF+WVDVS+PVL+SFVSKRVD+M+  G+++E  + F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSLLDEETQDELLQEAISKVKEHTCKLALRQLGKIQRLRNVNG 263
           GI+RAIGVPE   Y R +SL+D  T+ ++L  A+  +K++T  LA RQL KIQRL     
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFTKQG-KEAEEAWNKLVMAPSTTIIDQFLHPN 302
            ++HR+DATE F K+  +E +EAW  LV  PS  I+D+F + N
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

BLAST of Lag0002428 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 244.6 bits (623), Expect = 1.1e-64
Identity = 136/336 (40.48%), Postives = 203/336 (60.42%), Query Frame = 0

Query: 4   STMFMSKQITQQPLINLPPGRLNTNPFFNLRKLQ-------KEKVVIVIGATGTGKSRLS 63
           S  F S   +   L++    R    P  +  +++       K+KVV+++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINP-FSDFSATSFSD 123
           +D+ATRFPSE+INSDK+QVY+GL+I TN++T Q++RGVPHHLLG+INP   + +A  F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 MATLSLDAISGRDRLPVIAGGSNSYIETLIDSNFRSK----------------YECCFLW 183
            A+  +  I+ R ++P+IAGGSNS++  L+   F  K                YECCF+W
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSMPVLHSFVSKRVDKMVANGMVDEAEKFFDPV--GDYSR-GIKRAIGVPEFDSYFR- 243
           VDVS  VL+ ++ +RVD+M+ +GM +E  +F+DPV  G  +R GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YKSLLDEETQDELLQEAISKVKEHTCKLALRQLGKIQRLRNVNGWNIHRLDATEAF 302
                K +  +  +     +A+  +K +T  LA RQ+ KI+ L++  GW I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDA-GWEIERVDATASF 322

BLAST of Lag0002428 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 233.8 bits (595), Expect = 2.0e-61
Identity = 132/328 (40.24%), Postives = 201/328 (61.28%), Query Frame = 0

Query: 2   NVSTMFMSKQI--TQQPLINLPPGRLNTNPFFN--LRKLQKEKVVIVIGATGTGKSRLSI 61
           N+++ F+S  +     P + LPP R +  P     + +  K+KVV+++GATG+GKS LSI
Sbjct: 3   NLTSTFVSPSMIPITSPRLRLPPPR-SVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSI 62

Query: 62  DIATRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIINP-FSDFSATSFSDM 121
           D+ATRF  E++NSDK+Q YDGL + TN+++  E+ GVPHHLLG + P  S+ + + F  +
Sbjct: 63  DLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSL 122

Query: 122 ATLSLDAISGRDRLPVIAGGSNSYIETLIDSNFRSK---------------YECCFLWVD 181
           A+ S+  I+ R  LP+IAGGSNS+I  L+   F  K               YECCFLWVD
Sbjct: 123 ASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVD 182

Query: 182 VSMPVLHSFVSKRVDKMVANGMVDEAEKFFDPVGDYS----RGIKRAIGVPEFDSYF--- 241
           VS+ VL  ++SKRVD+M+ +GM +E   F+DP    S     GI + IG+PEFD YF   
Sbjct: 183 VSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLY 242

Query: 242 ----RYKSLLDEETQDELLQEAISKVKEHTCKLALRQLGKIQRLRNVNGWNIHRLDATEA 299
               + K    ++ +     EA+ ++KE+T +LA +Q+ +I +L++ +GW+I RLDAT +
Sbjct: 243 PPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKLKS-SGWDIQRLDATPS 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899604.13.1e-16589.09adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida][more]
KAG7025533.12.6e-16487.88Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper... [more]
XP_023514910.15.8e-16487.58adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
XP_022960175.13.8e-16387.88adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata][more]
XP_023004596.11.9e-16286.97adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q93WC91.2e-8751.97Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID21.5e-8557.09Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID12.8e-7650.18Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q5GHF73.8e-6543.22Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID31.6e-6340.48Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1H8C91.8e-16387.88adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1KSJ29.1e-16386.97adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A5D3DBM71.0e-15886.06Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BRC22.6e-15785.45adenylate isopentenyltransferase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A0A0K9194.4e-15785.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G63110.18.6e-8951.97isopentenyltransferase 3 [more]
AT5G19040.11.0e-8657.09isopentenyltransferase 5 [more]
AT3G23630.12.0e-7750.18isopentenyltransferase 7 [more]
AT1G68460.11.1e-6440.48isopentenyltransferase 1 [more]
AT3G19160.12.0e-6140.24ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01715IPPTcoord: 74..147
e-value: 1.6E-20
score: 73.9
coord: 151..248
e-value: 5.8E-12
score: 45.8
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 164..262
e-value: 8.9E-108
score: 361.5
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 35..300
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 38..298
e-value: 8.9E-108
score: 361.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 37..191
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 35..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002428.1Lag0002428.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
cellular_component GO:0009536 plastid
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity