Lag0002425 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002425 vs. NCBI nr
Match: XP_022959602.1 (basic 7S globulin 2-like [Cucurbita moschata])

HSP 1 Score: 734.2 bits (1894), Expect = 6.9e-208
Identity = 366/450 (81.33%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MGTRASILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGG 60
           M T    +F  F LLSFPFLSLQTAF+APIHKDHNS LYSISVHLKTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN Y+SSSYRLVKC+S L +S +  VCGSC++AP PICANDTIF  VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120

Query: 121 IRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNV 180
           IRD+YVDY + K TDSEN++TDVLALSTTDGS SG LR IS+ PF+C KT+FLRGL+RNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKS 240
           IGLAALG ANESIP  ISAKFNSPRIFA+CLSGTR GSGVAF GS+GPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN +AV FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVD 360
           TSTTYALLQ+SIYR FA AFMKEAA LNFTL NAVEPFGVCF   SV +TAAGP AP V 
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L +EKGKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E YRFGFSSSALL+GTSCS FNF+S+ +FL
Sbjct: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 450

BLAST of Lag0002425 vs. NCBI nr
Match: KAG7027263.1 (hypothetical protein SDJN02_11275, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 729.2 bits (1881), Expect = 2.2e-206
Identity = 362/450 (80.44%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MGTRASILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGG 60
           M T    +F  F LLSFPFLSLQTAF+APIHKDHNS LYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN Y+SSSYRLVKC+S L +S + +VC SC++AP  ICAND IF DVYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPS 120

Query: 121 IRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNV 180
           IRD+YVDY +PK TDSEN++TDVLALSTTDGS  G LR IS+ PF+C KT+FLRGL+RNV
Sbjct: 121 IRDEYVDYLNPKLTDSENVVTDVLALSTTDGSRPGLLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKS 240
           IGLAALG ANESIP  ISAKFNSPRIFA+CLSGTR GSGVAF GS+GPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN +AV FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVD 360
           TSTTYALL++SIYR FATAFMKEAA LNFTL NAVEPFGVCF   SVA+TAAGP AP V 
Sbjct: 301 TSTTYALLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVY 360

Query: 361 LVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L +EKGKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E +RFGFSSSALL+GTSCS FNF+S+ + L
Sbjct: 421 EKHRFGFSSSALLQGTSCSKFNFSSSDNLL 450

BLAST of Lag0002425 vs. NCBI nr
Match: XP_023514746.1 (basic 7S globulin 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 728.0 bits (1878), Expect = 4.9e-206
Identity = 361/450 (80.22%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MGTRASILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGG 60
           M T    +F  F LL FPFLSLQTAF+APIHKDHNS LYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLPFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN Y+SSSYRLVKC+S L +S + +VC SC++AP  ICAND IF DVYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPS 120

Query: 121 IRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNV 180
           IRD+YVDY +PK TDSEN++TDVLALSTTDGS SG LR IS+ PF+C KT+FLRGL+RNV
Sbjct: 121 IRDEYVDYLNPKLTDSENVVTDVLALSTTDGSRSGLLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKS 240
           IGLAALG ANESIP  ISAKFNSPRIFA+CLSGTR GSGVAF GS+GPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN +AV FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVD 360
           TSTTYALL++SIYR FATAFMKEAA LNFTL NAVEPFGVCF   SVA+TAAGP AP V 
Sbjct: 301 TSTTYALLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVY 360

Query: 361 LVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L +EKGKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E +RFGF+SSALL+GTSCS FNF+S+ + L
Sbjct: 421 EKHRFGFTSSALLQGTSCSKFNFSSSDNLL 450

BLAST of Lag0002425 vs. NCBI nr
Match: KAG6593130.1 (putative aspartic proteinase GIP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 726.9 bits (1875), Expect = 1.1e-205
Identity = 360/450 (80.00%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MGTRASILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGG 60
           M T    +F  F LLSFPFLSLQTAF+APIHKDHNS LYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN Y+SSSYRLVKC+S L +S + +VC SC++AP  ICAND IF DVYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPS 120

Query: 121 IRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNV 180
           IRD+YVDY +PK TDSEN++TDVLALSTTDGS SG LR IS+ PF+C KT+FLRGL+RN+
Sbjct: 121 IRDEYVDYLNPKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNI 180

Query: 181 IGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKS 240
           IGLAALG ANESIP  ISA+FNSPRIFA+CLSGTR GSGVAF GS+GPY FS NVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAEFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSTNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN +AV FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVD 360
           TSTTYALL++SIYR FATAFMKEAA LNFTL NAVEPFGVCF   SVA+TAAGP AP V 
Sbjct: 301 TSTTYALLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVY 360

Query: 361 LVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L +EKGKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E +RFGFSSSALL+GTSCS FNF+S+ + L
Sbjct: 421 EKHRFGFSSSALLQGTSCSKFNFSSSDNLL 450

BLAST of Lag0002425 vs. NCBI nr
Match: XP_023004536.1 (basic 7S globulin 2-like [Cucurbita maxima])

HSP 1 Score: 725.7 bits (1872), Expect = 2.4e-205
Identity = 360/450 (80.00%), Postives = 390/450 (86.67%), Query Frame = 0

Query: 1   MGTRASILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGG 60
           M T    +F  F LLSFPF SLQTAF+APIHKDHNS LYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFFSLQTAFIAPIHKDHNSLLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN Y+SSSYRLVKC+S L +S +  VCGSC++AP P C NDTIF  VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPTCGNDTIFTYVYPEKPS 120

Query: 121 IRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNV 180
           IRD+YVDY + K TDSEN++TDVLALSTTDGS SG LR +S  PF+C KT+FLRGL+RNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRQVSYMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKS 240
           IGLAALG ANESIP  ISAKFNSPRIFA+CLSGTR GSGVAFFGS+GPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFFGSKGPYIFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN + V FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEVVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVD 360
           TSTTYALLQ+SIYR FA AFMKEAA LNFTL NAVEPFGVCF   SV +TAAGP AP V 
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAARLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L +EKGKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E YRFGFSSSAL++GTSCS FNF+S+ +FL
Sbjct: 421 EKYRFGFSSSALMQGTSCSKFNFSSSDNFL 450

BLAST of Lag0002425 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 353.6 bits (906), Expect = 3.3e-96
Identity = 198/435 (45.52%), Postives = 271/435 (62.30%), Query Frame = 0

Query: 8   LFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGGAFSWIDC 67
           L I+F  L+  F   Q   +API KD  ++LY++SV LKTPL+P  LHL LG + SW+ C
Sbjct: 13  LAILFLFLTPTF---QIPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLC 72

Query: 68  YNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVD 127
            + Y+SSS   + C++ LCNS            P+  C+N++  C ++PE P  R+  +D
Sbjct: 73  DSTYTSSSSHHIPCNTPLCNSF-----------PSNACSNNSSLCALFPENPVTRNTLLD 132

Query: 128 YNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNVIGLAALG 187
                       + D LAL T D SSS  L  ISDF FSCA    L+GL+ N +GLA+LG
Sbjct: 133 ----------TALIDSLALPTYDASSS--LVLISDFIFSCATAHLLQGLAANALGLASLG 192

Query: 188 HANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGS-RGPYRFSPNVDLSKSLIYTPL 247
            +N S+P+ IS    SPR F +CL  + + +G A F S    + FS  +DL+    YT L
Sbjct: 193 RSNYSLPAQISTSLTSPRSFTLCLPASSANTGAAIFASTASSFLFSSKIDLT----YTQL 252

Query: 248 LFNPVSGSIYT-WWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTY 307
           + NPV+ ++ T    PS EY+I L++I+INGK +  N+S+L  +   G GGTKIST+  Y
Sbjct: 253 IVNPVADTVVTDNPQPSDEYFINLTSIKINGKPLYINSSILTVDQT-GFGGTKISTAEPY 312

Query: 308 ALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVDLVIEK 367
            +L+TSIYR F   F+ E++A N T+T AVEPFGVC+ A  +  T  GP  P VDLV+  
Sbjct: 313 TVLETSIYRLFVQRFVNESSAFNLTVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHS 372

Query: 368 GKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRF 427
             V W++ G NSMVR+ K GVD WCLGF++GG   RTPIVIGG Q+ED+L+QFDL++ RF
Sbjct: 373 EDVFWRIFGGNSMVRVAKGGVDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRF 416

Query: 428 GFSSSALLKGTSCSN 441
           GF+S+ LL+   CSN
Sbjct: 433 GFTSTLLLQDAKCSN 416

BLAST of Lag0002425 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 4.5e-85
Identity = 180/422 (42.65%), Postives = 246/422 (58.29%), Query Frame = 0

Query: 27  VAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNRYSSSSYRLVKCDSALC 86
           + PI KD  +  Y   +  +TPL P +L LDLGG F W+DC   Y SS+YR  +C SA C
Sbjct: 35  ILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQC 94

Query: 87  NSMDS-KVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVDYNHPKFTDSENLITDVLA 146
           +   +   CG C   P P C N+T  C + P+    R             S  L +D + 
Sbjct: 95  SLAGAGSGCGQCFSPPKPGCNNNT--CGLLPDNTITRT----------ATSGELASDTVQ 154

Query: 147 LSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNVIGLAALGHANESIPSMISAKFNSPR 206
           + +++G + G      DF F C  T  L GL+  V G+A LG    S+PS  SA+F+ PR
Sbjct: 155 VQSSNGKNPGRHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSFPR 214

Query: 207 IFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKS-LIYTPLLFNPVS-GSIYTWWLPSY 266
            FAVCLS + +  GV  FG  GPY F PN + + +   YTPL  NPVS  S ++   PS 
Sbjct: 215 KFAVCLSSSTNSKGVVLFGD-GPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREPSS 274

Query: 267 EYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMKE 326
           EY+IG+ +I+IN K VP NT+LL+ +   G GGTKIST   Y +L+TSIY      F+KE
Sbjct: 275 EYFIGVKSIKINEKVVPINTTLLSIDN-QGVGGTKISTVNPYTILETSIYNAVTNFFVKE 334

Query: 327 AAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVDLVIEKGKVVWKLGGRNSMVRIKK 386
              +N T   +V PF  CF + ++A T  GP  P++DLV++   V W++ G NSMV++ +
Sbjct: 335 --LVNITRVASVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANSMVQVSE 394

Query: 387 KGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALLKGTSCSNFNF 446
              +  CLGF++GG  PRT IV+GG  +ED+LLQFDL   R GF+SS L + T+C+NFNF
Sbjct: 395 ---NVLCLGFVDGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCANFNF 437

BLAST of Lag0002425 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 1.0e-65
Identity = 153/425 (36.00%), Postives = 228/425 (53.65%), Query Frame = 0

Query: 27  VAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNRYSSSSYRLVKCDSALC 86
           V  + KD  + L+ + +H +TPL      +DL G F  ++C N+Y+SS+Y+   C S+ C
Sbjct: 39  VLKVQKDRATNLHVVQIHKRTPLVQFPFVIDLTGRFLSVNCENQYTSSTYKAPVCHSSQC 98

Query: 87  NSMDSKVCGSCVEAPA-PICANDTIFCDVYPEKPSIRDQYVDYNHPKFTDSENLITDVLA 146
              +S  C +C  +   P C   T  C +    P  +           +    L  DVL 
Sbjct: 99  ARANSHTCRTCSSSKTRPGC--HTNACGLLTTNPVTQQ----------SAQGELAEDVLK 158

Query: 147 LSTTDGSSSGPLRPISDFPFSCAKTDFL-RGLSRNVIGLAALGHANESIPSMISAKFNSP 206
           + +T GSS GP+     F F+CA ++ L +GL +NV G+A LGH+  S+P  +++ F  P
Sbjct: 159 IPSTQGSSPGPMVTYPHFLFACAPSNILQKGLPKNVQGVAGLGHSPISLPYQLASHFGFP 218

Query: 207 RIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKSLIYTPLLFNPVSGSIYTWWLPSYE 266
             FAVCL+ +   +G  FFG  GPY   P +D+S+ L Y P                  E
Sbjct: 219 PKFAVCLTSSPGKNGAVFFG-EGPYFMKPGIDVSRQLTYAPFTIGQQG-----------E 278

Query: 267 YYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMKEA 326
           YYI + + +I       N ++L   P  G GG  IST+T Y  LQT I+R     FM + 
Sbjct: 279 YYINVQSFKI-------NNAMLPSIPKGGFGGAMISTTTPYTTLQTPIFRALNQLFMNQL 338

Query: 327 AALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVDLVIE-KGKVVWKLGGRNSMVRIKK 386
             +       V PFG CF A+ +  +  GPT P++DLV++ K  ++W++ G N+M++  +
Sbjct: 339 RGVPH--VKPVAPFGACFDANRIPTSKMGPTVPSIDLVLDNKKNIMWRIFGANAMIQ-PR 398

Query: 387 KGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALLKGTSCSNFNF 446
            GV   CL F++GG  P+ PIVIG  Q+ED+LLQFDL N R GFSSS L + T+C+NFNF
Sbjct: 399 PGV--MCLAFVDGGMRPKAPIVIGTQQLEDNLLQFDLMNSRLGFSSSLLFRRTNCANFNF 427

Query: 447 TSAQS 449
            ++ +
Sbjct: 459 GTSST 427

BLAST of Lag0002425 vs. ExPASy Swiss-Prot
Match: Q8RVH5 (Basic 7S globulin 2 OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 4.7e-50
Identity = 144/428 (33.64%), Postives = 214/428 (50.00%), Query Frame = 0

Query: 27  VAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNRYSSSSYRLVKCDSALC 86
           V P+  D ++ L+  ++  +TPL    + +DL G   W++C   YSS +Y+   C S  C
Sbjct: 42  VLPVQNDASTGLHWANLQKRTPLMQVPVLVDLNGNHLWVNCEQHYSSKTYQAPFCHSTQC 101

Query: 87  NSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVDYNHPKFTDSENLITDVLAL 146
           +  ++  C SC  A  P C  +T  C +    P  +           T    L  DVLA+
Sbjct: 102 SRANTHQCLSCPAASRPGCHKNT--CGLMSTNPITQQ----------TGLGELGQDVLAI 161

Query: 147 STTDGSSS--GPLRPISDFPFSCAKTDFL-RGLSRNVIGLAALGHANESIPSMISAKFNS 206
             T GS+   GPL  +  F FSCA +  L +GL RN+ G+A LGHA  S+P+ +++ F  
Sbjct: 162 HATQGSTQQLGPLVTVPQFLFSCAPSFLLQKGLPRNIQGVAGLGHAPISLPNQLASHFGL 221

Query: 207 PRIFAVCLSGTRSGSGVAFFGSRGPYRFSP--NVDLSKSLIYTPLLFNPVSGSIYTWWLP 266
              F  CLS   +  G   FG   P       N D+   L +TPL   P           
Sbjct: 222 QHQFTTCLSRYPTSKGALIFGD-APNNMQQFHNQDIFHDLAFTPLTVTPQG--------- 281

Query: 267 SYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFM 326
             EY + +S+IRIN  +V F  + ++   V   GGT ISTST + +LQ S+Y+ F   F 
Sbjct: 282 --EYNVRVSSIRINQHSV-FPPNKISSTIVGSSGGTMISTSTPHMVLQQSLYQAFTQVFA 341

Query: 327 KEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVDLVIEK-GKVVWKLGGRNSMVR 386
           ++          +V PFG+CF ++ +         P+VDLV++K    VW++ G + MV+
Sbjct: 342 QQLE--KQAQVKSVAPFGLCFNSNKI------NAYPSVDLVMDKPNGPVWRISGEDLMVQ 401

Query: 387 IKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSAL-LKGTSCS 446
             + GV   CLG +NGG  PR  + +G  Q+E+ L+ FDL   R GFS+S+L   G  C 
Sbjct: 402 -AQPGVT--CLGVMNGGMQPRAEVTLGTRQLEEKLMVFDLARSRVGFSTSSLHSHGVKCG 433

BLAST of Lag0002425 vs. ExPASy Swiss-Prot
Match: P13917 (Basic 7S globulin OS=Glycine max OX=3847 GN=BG PE=1 SV=2)

HSP 1 Score: 195.7 bits (496), Expect = 1.2e-48
Identity = 142/427 (33.26%), Postives = 216/427 (50.59%), Query Frame = 0

Query: 27  VAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNRYSSSSYRLVKCDSALC 86
           V P+  D ++ L+  ++  +TPL    + +DL G   W++C  +YSS +Y+   C S  C
Sbjct: 35  VLPVQNDGSTGLHWANLQKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQC 94

Query: 87  NSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVDYNHPKFTDSENLITDVLAL 146
           +  ++  C SC  A  P C  +T  C +    P  +           T    L  DVLA+
Sbjct: 95  SRANTHQCLSCPAASRPGCHKNT--CGLMSTNPITQQ----------TGLGELGEDVLAI 154

Query: 147 STTDGSSS--GPLRPISDFPFSCAKTDFL-RGLSRNVIGLAALGHANESIPSMISAKFNS 206
             T GS+   GPL  +  F FSCA +  + +GL RN  G+A LGHA  S+P+ +++ F  
Sbjct: 155 HATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGL 214

Query: 207 PRIFAVCLSGTRSGSGVAFFG-SRGPYRFSPNVDLSKSLIYTPLLFNPVSGSIYTWWLPS 266
            R F  CLS   +  G   FG +    R   N D+   L +TPL    + G         
Sbjct: 215 QRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTIT-LQG--------- 274

Query: 267 YEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMK 326
            EY + +++IRIN  +V     + +       GGT ISTST + +LQ S+Y+ F   F +
Sbjct: 275 -EYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQ 334

Query: 327 EAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVDLVIEK-GKVVWKLGGRNSMVRI 386
           +          +V PFG+CF ++ +         P+VDLV++K    VW++ G + MV+ 
Sbjct: 335 QLP--KQAQVKSVAPFGLCFNSNKI------NAYPSVDLVMDKPNGPVWRISGEDLMVQ- 394

Query: 387 KKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSAL-LKGTSCSN 446
            + GV   CLG +NGG  PR  I +G  Q+E++L+ FDL   R GFS+S+L   G  C++
Sbjct: 395 AQPGVT--CLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFSTSSLHSHGVKCAD 427

BLAST of Lag0002425 vs. ExPASy TrEMBL
Match: A0A6J1H501 (basic 7S globulin 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460627 PE=4 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.3e-208
Identity = 366/450 (81.33%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 1   MGTRASILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGG 60
           M T    +F  F LLSFPFLSLQTAF+APIHKDHNS LYSISVHLKTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN Y+SSSYRLVKC+S L +S +  VCGSC++AP PICANDTIF  VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120

Query: 121 IRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNV 180
           IRD+YVDY + K TDSEN++TDVLALSTTDGS SG LR IS+ PF+C KT+FLRGL+RNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKS 240
           IGLAALG ANESIP  ISAKFNSPRIFA+CLSGTR GSGVAF GS+GPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN +AV FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVD 360
           TSTTYALLQ+SIYR FA AFMKEAA LNFTL NAVEPFGVCF   SV +TAAGP AP V 
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L +EKGKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E YRFGFSSSALL+GTSCS FNF+S+ +FL
Sbjct: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 450

BLAST of Lag0002425 vs. ExPASy TrEMBL
Match: A0A6J1KSD2 (basic 7S globulin 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497811 PE=4 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 1.2e-205
Identity = 360/450 (80.00%), Postives = 390/450 (86.67%), Query Frame = 0

Query: 1   MGTRASILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGG 60
           M T    +F  F LLSFPF SLQTAF+APIHKDHNS LYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFFSLQTAFIAPIHKDHNSLLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 120
           AFSWIDCYN Y+SSSYRLVKC+S L +S +  VCGSC++AP P C NDTIF  VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPTCGNDTIFTYVYPEKPS 120

Query: 121 IRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNV 180
           IRD+YVDY + K TDSEN++TDVLALSTTDGS SG LR +S  PF+C KT+FLRGL+RNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRQVSYMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKS 240
           IGLAALG ANESIP  ISAKFNSPRIFA+CLSGTR GSGVAFFGS+GPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFFGSKGPYIFSPNVDLSKS 240

Query: 241 LIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKIS 300
           LIYTPLLFNP SGSIYT WLPSYEYYIGLSAIRIN + V FNTSLL FEPVHG GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEVVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVD 360
           TSTTYALLQ+SIYR FA AFMKEAA LNFTL NAVEPFGVCF   SV +TAAGP AP V 
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAARLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L +EKGKVVWKLGGRNSMVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           E YRFGFSSSAL++GTSCS FNF+S+ +FL
Sbjct: 421 EKYRFGFSSSALMQGTSCSKFNFSSSDNFL 450

BLAST of Lag0002425 vs. ExPASy TrEMBL
Match: A0A5D3D4L4 (Basic 7S globulin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2032G00050 PE=4 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.9e-196
Identity = 338/440 (76.82%), Postives = 381/440 (86.59%), Query Frame = 0

Query: 7   ILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGGAFSWID 66
           + F  FFL+SFP  SLQTA VAP+ KDH S LYSIS+HLKTPLRPA L+LDLGG FSWID
Sbjct: 6   LFFFFFFLISFPSYSLQTASVAPLFKDHISLLYSISLHLKTPLRPATLYLDLGGPFSWID 65

Query: 67  CYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYV 126
           CY  Y+SSSY+L+ C++ L NS +  +CGSCV+AP+P CANDTIF   YP+ PS+RDQ+V
Sbjct: 66  CYQNYNSSSYKLLLCNTPLSNSFNQGICGSCVQAPSPTCANDTIFTYAYPQNPSLRDQFV 125

Query: 127 DYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNVIGLAAL 186
           DY+ P+ TDSEN+ITDVLALSTTDGS SGPLR IS+ PF+C KT+FLRGL++NVIGLAAL
Sbjct: 126 DYDRPELTDSENVITDVLALSTTDGSKSGPLRRISEIPFACVKTNFLRGLAKNVIGLAAL 185

Query: 187 GHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKSLIYTPL 246
           G +N SIPS+ISAKFNSP+ FA+CLSG RSG GVAFFGS+GPYRFSPNVDLSKSL YTPL
Sbjct: 186 GRSNLSIPSVISAKFNSPKFFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKSLTYTPL 245

Query: 247 LFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYA 306
           LFNP SGSI+T+WLPSYEYY+GLSAIRINGK VPFNTSLL FEP+HG GG KISTST Y 
Sbjct: 246 LFNPASGSIHTYWLPSYEYYVGLSAIRINGKVVPFNTSLLPFEPIHGNGGAKISTSTNYG 305

Query: 307 LLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAG-PTAPAVDLVIEK 366
           LL++SIYR FA  FMKEAAALNF L NAV+PFGVC+AA SV VTA G   AP VDLV+EK
Sbjct: 306 LLESSIYRAFARVFMKEAAALNFKLINAVKPFGVCYAAKSVGVTAEGHAKAPVVDLVMEK 365

Query: 367 GKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRF 426
           GKVVWKLGGRN+MVRIKKKGVDAWCLGFINGGEFPRTPIV+GGLQMEDHLLQFDLE +RF
Sbjct: 366 GKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVMGGLQMEDHLLQFDLEKFRF 425

Query: 427 GFSSSALLKGTSCSNFNFTS 446
           GFSSSAL +GTSCS FNF S
Sbjct: 426 GFSSSALTEGTSCSKFNFNS 445

BLAST of Lag0002425 vs. ExPASy TrEMBL
Match: A0A0A0K506 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G390050 PE=4 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 6.5e-196
Identity = 337/443 (76.07%), Postives = 383/443 (86.46%), Query Frame = 0

Query: 7   ILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGGAFSWID 66
           + F  FFL+SFP  SLQTA +AP++K H S LYSIS+HLKTPLRPA+L+LDLGGAFSWI 
Sbjct: 6   LFFFFFFLISFPLYSLQTALIAPLYKHHTSLLYSISLHLKTPLRPASLYLDLGGAFSWIH 65

Query: 67  CYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYV 126
           CY  Y+SSSY+ V C++ L NS +  +CGSCV+AP+PICANDTIF   YPE PS+RD +V
Sbjct: 66  CYQNYNSSSYKFVLCNTPLSNSFNQAICGSCVQAPSPICANDTIFSYAYPENPSLRDHFV 125

Query: 127 DYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNVIGLAAL 186
           DY+HPK TDSEN+ITDVLALSTT GS+S PLR I +FPF+C KT+FLR +++NVIGLAAL
Sbjct: 126 DYDHPKLTDSENVITDVLALSTTGGSTSAPLRRIPEFPFACVKTNFLREVAKNVIGLAAL 185

Query: 187 GHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKSLIYTPL 246
           G +N SIPS+ISAKF+SP+ FA+CLSG RSG GVAFFGS+GPYRFSPNVDLSKSL YTPL
Sbjct: 186 GRSNLSIPSVISAKFSSPKYFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKSLTYTPL 245

Query: 247 LFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYA 306
           LFNPVS SIYT+WLPSYEYY+GLSAIRINGK VPFNTSLL+FEP+HG GG KISTST YA
Sbjct: 246 LFNPVSASIYTYWLPSYEYYVGLSAIRINGKVVPFNTSLLSFEPIHGRGGAKISTSTNYA 305

Query: 307 LLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPT-APAVDLVIEK 366
           LL++SIYR FAT FMKEA  LNF L NAVEPFGVC+ A SV VTA G   AP VDLV+EK
Sbjct: 306 LLRSSIYRAFATVFMKEAVVLNFKLINAVEPFGVCYEAKSVGVTAEGQAKAPVVDLVMEK 365

Query: 367 GKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRF 426
            KVVWKLGGRN+MVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLEN+RF
Sbjct: 366 EKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENFRF 425

Query: 427 GFSSSALLKGTSCSNFNFTSAQS 449
           GFSSSAL +GTSCS F+FTSA +
Sbjct: 426 GFSSSALKEGTSCSKFDFTSANN 448

BLAST of Lag0002425 vs. ExPASy TrEMBL
Match: A0A6J1D7R8 (LOW QUALITY PROTEIN: basic 7S globulin 2 OS=Momordica charantia OX=3673 GN=LOC111018418 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.5e-168
Identity = 304/409 (74.33%), Postives = 335/409 (81.91%), Query Frame = 0

Query: 45  LKTPLRPANLHLDLGGAFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPI 104
           +KTPLRP  LHLDLGGAFSWIDCYN Y+SSSYRLV  +SALCNSM   + G CV+AP P 
Sbjct: 1   MKTPLRPTKLHLDLGGAFSWIDCYNHYNSSSYRLVDSESALCNSMHPNIAGPCVDAPTPF 60

Query: 105 CAND--TIFCDVYPEK-PSIRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPIS 164
           C+N+  T+FC VYPEK PSIRD  VDY+H K TDSENLI DVLALSTTDGS+ GPLR I 
Sbjct: 61  CSNNGTTLFCYVYPEKPPSIRDS-VDYDHVKLTDSENLIVDVLALSTTDGSNPGPLRQIP 120

Query: 165 DFPFSCAKTDFLRGLSRNVIGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVA 224
             P SC           +VIGL ALG ANESIPSM+S  F   + FAVCL G RSG GVA
Sbjct: 121 HLPISC----------XDVIGLVALGXANESIPSMVSTTFGRSKYFAVCLPGARSGPGVA 180

Query: 225 FFGSRGPYRFSPNVDLSKSLIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPF 284
           FFGS+GPY+FSPNVDLSKSLIYTPLLFNPVS SIYT+WLPSYEYYI LSAIRIN K VPF
Sbjct: 181 FFGSKGPYKFSPNVDLSKSLIYTPLLFNPVSASIYTYWLPSYEYYIALSAIRINAKTVPF 240

Query: 285 NTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVC 344
           NTSLL FEP+HGGGGTKISTS TYALLQTSIYR FA AF KE+AALNFT T+ V+PFG+C
Sbjct: 241 NTSLLPFEPIHGGGGTKISTSATYALLQTSIYRAFAAAFAKESAALNFTATDPVKPFGLC 300

Query: 345 FAADSVAVTAAGPTAPAVDLVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPR 404
           +AA+SVA+TAAGP APAVDLV+E GK  W+LGGRNSMVR+KK GVDAWCLGFI+G E PR
Sbjct: 301 YAAESVAMTAAGPAAPAVDLVMEGGKSAWRLGGRNSMVRMKKVGVDAWCLGFIDGVENPR 360

Query: 405 TPIVIGGLQMEDHLLQFDLENYRFGFSSSALLKGTSCSNFNFTSAQSFL 451
           TPIVIGGLQMED LLQFDLEN RFGF SS LL+GTSCS FNFTS  +F+
Sbjct: 361 TPIVIGGLQMEDQLLQFDLENSRFGFGSSVLLQGTSCSKFNFTSLDNFI 398

BLAST of Lag0002425 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 315.5 bits (807), Expect = 7.1e-86
Identity = 185/446 (41.48%), Postives = 266/446 (59.64%), Query Frame = 0

Query: 6   SILFIIFFLLSFPFLSLQTAF-----VAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGG 65
           S+L +  F LS    S QT F     + P+ KD ++  Y+  ++ +TPL PA++  DLGG
Sbjct: 9   SVLLLFIFSLS---SSAQTPFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGG 68

Query: 66  AFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPS 125
              W+DC   Y SS+Y+  +C+SA+C+   S  CG+C   P P C+N+T  C   P    
Sbjct: 69  RELWVDCDKGYVSSTYQSPRCNSAVCSRAGSTSCGTCFSPPRPGCSNNT--CGGIP---- 128

Query: 126 IRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNV 185
                 D        S     DV+++ +T+GS+ G +  I +  F C  T  L+GL++  
Sbjct: 129 ------DNTVTGTATSGEFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGLAKGT 188

Query: 186 IGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVDLSKS 245
           +G+A +G  N  +PS  +A F+  R FAVCL+   SG GVAFFG+ GPY F P + +S S
Sbjct: 189 VGMAGMGRHNIGLPSQFAAAFSFHRKFAVCLT---SGKGVAFFGN-GPYVFLPGIQIS-S 248

Query: 246 LIYTPLLFNPVS-GSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKI 305
           L  TPLL NPVS  S ++    S EY+IG++AI+I  K VP N +LL      G GGTKI
Sbjct: 249 LQTTPLLINPVSTASAFSQGEKSSEYFIGVTAIQIVEKTVPINPTLLKINASTGIGGTKI 308

Query: 306 STSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAV 365
           S+   Y +L++SIY  F + F+K+AAA +     +V+PFG CF+  +V VT  G   P +
Sbjct: 309 SSVNPYTVLESSIYNAFTSEFVKQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEI 368

Query: 366 DLVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFD 425
           +LV+    VVW++ G NSMV +     D  CLGF++GG   RT +VIGG Q+ED+L++FD
Sbjct: 369 ELVLHSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNARTSVVIGGFQLEDNLIEFD 428

Query: 426 LENYRFGFSSSALLKGTSCSNFNFTS 446
           L + +FGFSS+ L + T+C+NFNFTS
Sbjct: 429 LASNKFGFSSTLLGRQTNCANFNFTS 431

BLAST of Lag0002425 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 305.8 bits (782), Expect = 5.6e-83
Identity = 177/451 (39.25%), Postives = 265/451 (58.76%), Query Frame = 0

Query: 5   ASILFIIFFLLSFPFLSLQT---------AFVAPIHKDHNSRLYSISVHLKTPLRPANLH 64
           AS   IIF +L     SL +         A + P+ KD ++  Y+  ++ +TPL PA++ 
Sbjct: 2   ASSRIIIFSVLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASVV 61

Query: 65  LDLGGAFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVY 124
            DLGG   W+DC   Y S++YR  +C+SA+C+   S  CG+C   P P C+N+T  C  +
Sbjct: 62  FDLGGREFWVDCDQGYVSTTYRSPRCNSAVCSRAGSIACGTCFSPPRPGCSNNT--CGAF 121

Query: 125 PEKPSIRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRG 184
           P          D +   +  S     DV+++ +T+GS+ G    I +  FSC  T  L+G
Sbjct: 122 P----------DNSITGWATSGEFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLKG 181

Query: 185 LSRNVIGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNV 244
           L++  +G+A +G  N  +P   +A F+  R FAVCL+   SG GVAFFG+ GPY F P +
Sbjct: 182 LAKGAVGMAGMGRHNIGLPLQFAAAFSFNRKFAVCLT---SGRGVAFFGN-GPYVFLPGI 241

Query: 245 DLSKSLIYTPLLFNPVSGSI-YTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGG 304
            +S+ L  TPLL NP +    ++    S EY+IG++AI+I  K +P + +LL      G 
Sbjct: 242 QISR-LQKTPLLINPGTTVFEFSKGEKSPEYFIGVTAIKIVEKTLPIDPTLLKINASTGI 301

Query: 305 GGTKISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGP 364
           GGTKIS+   Y +L++SIY+ F + F+++AAA +     +V+PFG CF+  +V VT  G 
Sbjct: 302 GGTKISSVNPYTVLESSIYKAFTSEFIRQAAARSIKRVASVKPFGACFSTKNVGVTRLGY 361

Query: 365 TAPAVDLVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDH 424
             P + LV+    VVW++ G NSMV +     D  CLGF++GG  P   +VIGG Q+ED+
Sbjct: 362 AVPEIQLVLHSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNPGASVVIGGFQLEDN 421

Query: 425 LLQFDLENYRFGFSSSALLKGTSCSNFNFTS 446
           L++FDL + +FGFSS+ L + T+C+NFNFTS
Sbjct: 422 LIEFDLASNKFGFSSTLLGRQTNCANFNFTS 432

BLAST of Lag0002425 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.8e-44
Identity = 134/448 (29.91%), Postives = 207/448 (46.21%), Query Frame = 0

Query: 1   MGTRASILFIIFFLLSFPFLSLQTAFVAPIHKDHNSRLYSISVHLKTPLR-PANLHLDLG 60
           MG+  + L +   + +   L   + ++ PI K   + L+  + ++ +  + P NL LDLG
Sbjct: 1   MGSSLTRLLVFLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLG 60

Query: 61  GAFSWIDCYNRYSSSSYRLVKCDSALCNSMDSKVCG--SCV-EAPAPICANDTIFCDVYP 120
              +W+DC    S SS RLV C S+ C S+    C   SC+ + P P+  N  +      
Sbjct: 61  TNLTWLDCRKLKSLSSLRLVTCQSSTCKSIPGNGCAGKSCLYKQPNPLGQNPVV------ 120

Query: 121 EKPSIRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGL 180
                              +  ++ D  +L TTDG        +  F FSCA    L+GL
Sbjct: 121 -------------------TGRVVQDRASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGL 180

Query: 181 SRNVIGLAALGHANESIPSMISAKFNSPRIFAVCLSGTRSGSGVAFFGSRGPYRFSPNVD 240
              V G+ AL   + S    +++ FN    F++CL      SG   F   G + F P  +
Sbjct: 181 PPPVDGVLALSPGSSSFTKQVTSAFNVIPKFSLCL----PSSGTGHFYIAGIHYFIPPFN 240

Query: 241 LSKSLIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGG 300
            S + I  P    P+ G+       S +Y I + +I + G A+  N  LL        GG
Sbjct: 241 SSDNPI--PRTLTPIKGT------DSGDYLITVKSIYVGGTALKLNPDLLT-------GG 300

Query: 301 TKISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVT-AAGPT 360
            K+ST   Y +LQT IY   A +F  +A A+      +V PF  CF + +      AGP 
Sbjct: 301 AKLSTVVHYTVLQTDIYNALAQSFTLKAKAMGIAKVPSVAPFKHCFDSRTAGKNLTAGPN 360

Query: 361 APAVDLVI--EKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMED 420
            P +++ +    G+V W   G N++V++K+      CL FI+GG+ P+  +VIG  Q++D
Sbjct: 361 VPVIEIGLPGRIGEVKWGFYGANTVVKVKE---TVMCLAFIDGGKTPKDLMVIGTHQLQD 401

Query: 421 HLLQFDLENYRFGFSSSALLKGTSCSNF 442
           H+L+FD       FS S LL  TSCS +
Sbjct: 421 HMLEFDFSGTVLAFSESLLLHNTSCSTW 401

BLAST of Lag0002425 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 171.0 bits (432), Expect = 2.2e-42
Identity = 145/444 (32.66%), Postives = 205/444 (46.17%), Query Frame = 0

Query: 10  IIFFLLSFPFLSLQT---------AFVAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGG 69
           +IF LLS  FL L           +F+ PI+KD    +Y+I + + +        LDL G
Sbjct: 5   VIFLLLSLVFLYLANTSHSLRKFQSFLHPIYKDTAKNIYTIPLSIGS-TSSEKFVLDLNG 64

Query: 70  AFSWI-DCYNRYSSSSYRLVKCDSALCNSMDSKVCGSCVEAPAPICANDTIFCDVYPEKP 129
           A   + +C     S++Y  ++C S  C     K        P  + A     C       
Sbjct: 65  AAPLLQNCPTAAKSTTYHPIRCGSTRC-----KYANPNFPCPNNVIAKKRTVC------- 124

Query: 130 SIRDQYVDYNHPKFTDSENLITDVLALSTTDGSSSGPLRPISDFPFSCAKTDFLRGLSRN 189
                    N   F D+  L+     + T D   S      S    +C  TD    L + 
Sbjct: 125 -----LSSDNSRLFRDTVPLLYTFNGVYTRDSEMS------SSLTLTC--TDGAPALKQR 184

Query: 190 VIGLAALGHANESIPSMISAKFNSPRIFAVCLSGT---RSGSGVAFFGSRGPYRFSP-NV 249
            IGLA   + + SIPS + + +  P   A+CL  T   +S +G  + G +G Y + P + 
Sbjct: 185 TIGLA---NTHLSIPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIG-KGEYYYLPYDK 244

Query: 250 DLSKSLIYTPLLFNPVSGSIYTWWLPSYEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGG 309
           D+SK    TPL+ N  SG          EY I + +I+I  K VP              G
Sbjct: 245 DVSKIFASTPLIGNGKSG----------EYLIDVKSIQIGAKTVPIPY-----------G 304

Query: 310 GTKISTSTTYALLQTSIYRGFATAFMKEAAALNFTLTNAVEPFGVCFAADSVAVTAAGPT 369
            TKIST   Y + QTS+Y+   TAF +    +      AV+PFG CF ++       G  
Sbjct: 305 ATKISTLAPYTVFQTSLYKALLTAFTEN---IKIAKAPAVKPFGACFYSN------GGRG 364

Query: 370 APAVDLVIEKGKVVWKLGGRNSMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHL 429
            P +DLV+  G   W++ G NS+V++ K  V   CLGF++GG  P+ PIVIGG QMED+L
Sbjct: 365 VPVIDLVL-SGGAKWRIYGSNSLVKVNKNVV---CLGFVDGGVKPKYPIVIGGFQMEDNL 384

Query: 430 LQFDLENYRFGFSSSALLKGTSCS 440
           ++FDLE  +F FSSS LL  TSCS
Sbjct: 425 VEFDLEASKFSFSSSLLLHNTSCS 384

BLAST of Lag0002425 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 154.8 bits (390), Expect = 1.6e-37
Identity = 132/415 (31.81%), Postives = 190/415 (45.78%), Query Frame = 0

Query: 27  VAPIHKDHNSRLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNRYSSSSYRLVKCDSALC 86
           V P+ KD  +  Y   + L     P  L +DL G+  W DC +R+ SSS  L+   S+ C
Sbjct: 33  VFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSILWFDCSSRHVSSSRNLISGSSSGC 92

Query: 87  NSMDSKVCGSCVEAPAPICANDTIFCDVYPEKPSIRDQYVDYNHPKFTDSENLITDVLAL 146
             + +KV    V + +    +    C++  +  +             T    L +DV+++
Sbjct: 93  --LKAKVGNERVSSSSSSRKDQNADCELLVKNDAF----------GITARGELFSDVMSV 152

Query: 147 STTDGSSSGPLRPISDFPFSCAKTDFLRGLSRNVIGLAALGHANESIPSMISAKFNSPRI 206
               GS + P     D  F+C     LRGL+    G+  LG A  S+PS ++A+ N  R 
Sbjct: 153 ----GSVTSP--GTVDLLFACTPPWLLRGLASGAQGVMGLGRAQISLPSQLAAETNERRR 212

Query: 207 FAVCLS---GTRSGSGV-AFFGSRGPYRFSPNVDLSKSLIYTPLLFNPVSGSIYTWWLPS 266
             V LS   G  S S V   FG          V  S+SL+YTPLL    +GS       S
Sbjct: 213 LTVYLSPLNGVVSTSSVEEVFG----------VAASRSLVYTPLL----TGS-------S 272

Query: 267 YEYYIGLSAIRINGKAVPFNTSLLAFEPVHGGGGTKISTSTTYALLQTSIYRGFATAFMK 326
             Y I + +IR+NG+ +           V G    ++ST   Y +L++SIY+ FA A+ K
Sbjct: 273 GNYVINVKSIRVNGEKL----------SVEGPLAVELSTVVPYTILESSIYKVFAEAYAK 332

Query: 327 EAAALNFTLTNAVEPFGVCFAADSVAVTAAGPTAPAVDLVIEKGKVVWKLGGRNSMVRIK 386
             AA   T    V PFG+CF +D           PAVDL ++   V W++ G+N MV + 
Sbjct: 333 --AAGEATSVPPVAPFGLCFTSD--------VDFPAVDLALQSEMVRWRIHGKNLMVDV- 385

Query: 387 KKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALLKGTS 438
             G    C G ++GG     PIV+GGLQ+E  +L FDL N   GF        TS
Sbjct: 393 --GGGVRCSGIVDGGSSRVNPIVMGGLQLEGFILDFDLGNSMMGFGQRTRSDSTS 385