Lag0002313 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002313
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Location	chr4: 41574294 .. 41576319 (-)
RNA-Seq Expression	Lag0002313
Synteny	Lag0002313

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAATCAAAACAAGAGGCAAAGGAGATGGTGAAGTCTCCTCCAAGGCTCTATAGGAGGAAAAGTTGGACCAAGATGGATAAAAATGTTGCTTTCTTCTTTCTCATTTTGCATCTACTTTGTCTTTTGGCCCCGTTCGATTTCAGTTGGAGTGGTTTTTGGCTTGCCATCGTAGTAGGCAATGCTACAGGTATGGCAATTAGTGTTTCGTTTCATAGAAATCTTGCACATCGAAGCTTCAAACTTCCCAAATGGCTTGAGTATTCGCTTGCTTATTGTGCATCTCATGCACTTCAGGTTTGATATTTTGAATCCTTTCTCTTAATTAAAAATGAACTAAGCTAGGAATAAATGTGATTCTGAACTGCGTAATATGCAAGGTGTACACATTATTCTTATTTTGTAGTGTAAAAATGATTATATATTTATAACATATATGTAGTAACTTGCATAACACCTTATATCATCAAATCAAAAAGTTAGCTTTTTTACTATTGTATAAAAAAAAGAGTGACCTAAGAGCATAGCTCAACTGACATAAAGTTGTACTAATAACCACGAAGCCTGTGGTTCAAATCCCCACACCCCGATATTGTCAAAAAAAAAGAAAAAAAGAAAGAATGCCTTGTGTGATTGTTGCATGTCAATAAAAACATTGACAATGAGGCCCACATGGATTTTTTTTTTCTTTTCCTTTATTTTTTTTTTATTTTTTTTTGAACTAGAAGATGAGCTTGCCATAAAACTAGTGAAAAAACTATAGTATCATCCTCAAATATGCTTTGTTAATTATCTTTGGTCTCTTAACCTGTCTTTTATGTTTGGTAACATTTTCGTTGATTTCTGAATTTTAGGGTGATCCAATTGATTGGGTGAGCACACACAGGTGCCATCATCGATATGTTGATACAGAACGAGACCCACATAGTCCTATATATGGATTTTGGTTTAGTCAAATAACATGGTTTTTCGATTCCTATAACTTGACTAGAAAAGTGTGCCCAAATTATTTCAATAACTTTCAAAAGGTAGAAAGAAATAGCTTTGTGTTGTTCTTGAAGCATGGGTATCCAGACAATGTCAACGACTTGGAGAGACAAGCTTTCTATAGGTTTATGCACAAAACATATATGCTTCATCCACTTGCTCTTTCAGCATTGTTGTATGCCATCGGAGGATTTCATTTTATTCTATGGGGAATGGTAAAATGAATCTACTCTCTCATTCTTTTTCCTTTTAATTATATGAATGAAAGCAAATATAATATCGTTGAACTCAAAAAAGAAAGATATACTAGTTTTGGTATAATTCTCTTGTATTAAATATTTTACTTACGAGAGTCCTTTTTTTGTTTTTTTGTTTTTTTGTTTTTTTTTTTTTTTACTATTCATAGTGTGTGAGGTACATTCTGAGCTTGCATATAACATCTATGATAAATTCAGTATGTCATATATGGGGAAAACAACCATGGAATACTGGTGATTTGTCTCGAAACAATTGGTAAGATACTTATCCATCTCATTTTATCTATATCCTTTACAATTCTCTCTCTTTCATCCAACGGTGCAAAACTACAGACTTCATTGCACTGGAAACATGTAAAAATTTCTAGATGACAAAAGTTTATAGGGAAATCACTCACCTTAACCTAAGACATATAACTCCAAACATTTTTTTTTAGACATGTATTGATTAGAAAAAGTGAATAAAAGATTTGTTTATATCAATTTAATTTATTAAACATTAATAGTAATTAGAAAAGTGATTTTTGTTTAATATTATTTTTGTTATTAGGTTGGTGGCTTTATTTACCTTTGGAGAAGGATGGCATAATAACCACCATGCTTTTGAGTATTCAGCAAGGCATGGTCATGAATGGTGGCAGATTGATTTTGGTTGGTACATTATTAAGTTTCTTCATGTCATTGGACTGGCAACTAATGTGAAATTACCTTCTCCAACCAAAAAGTTGGCAGCTTTGGACAAATCAAAACTCACCCTTCTTAAGCCTATGATTAATTAA

mRNA sequence

ATGGAAGAATCAAAACAAGAGGCAAAGGAGATGGTGAAGTCTCCTCCAAGGCTCTATAGGAGGAAAAGTTGGACCAAGATGGATAAAAATGTTGCTTTCTTCTTTCTCATTTTGCATCTACTTTGTCTTTTGGCCCCGTTCGATTTCAGTTGGAGTGGTTTTTGGCTTGCCATCGTAGTAGGCAATGCTACAGGTATGGCAATTAGTGTTTCGTTTCATAGAAATCTTGCACATCGAAGCTTCAAACTTCCCAAATGGCTTGAGTATTCGCTTGCTTATTGTGCATCTCATGCACTTCAGGGTGATCCAATTGATTGGGTGAGCACACACAGGTGCCATCATCGATATGTTGATACAGAACGAGACCCACATAGTCCTATATATGGATTTTGGTTTAGTCAAATAACATGGTTTTTCGATTCCTATAACTTGACTAGAAAAGTGTGCCCAAATTATTTCAATAACTTTCAAAAGGTAGAAAGAAATAGCTTTGTGTTGTTCTTGAAGCATGGGTATCCAGACAATGTCAACGACTTGGAGAGACAAGCTTTCTATAGGTTTATGCACAAAACATATATGCTTCATCCACTTGCTCTTTCAGCATTGTTGTATGCCATCGGAGGATTTCATTTTATTCTATGGGGAATGTGTGTGAGGTACATTCTGAGCTTGCATATAACATCTATGATAAATTCAGTATGTCATATATGGGGAAAACAACCATGGAATACTGGTGATTTGTCTCGAAACAATTGGTTGGTGGCTTTATTTACCTTTGGAGAAGGATGGCATAATAACCACCATGCTTTTGAGTATTCAGCAAGGCATGGTCATGAATGGTGGCAGATTGATTTTGGTTGGTACATTATTAAGTTTCTTCATGTCATTGGACTGGCAACTAATGTGAAATTACCTTCTCCAACCAAAAAGTTGGCAGCTTTGGACAAATCAAAACTCACCCTTCTTAAGCCTATGATTAATTAA

Coding sequence (CDS)

ATGGAAGAATCAAAACAAGAGGCAAAGGAGATGGTGAAGTCTCCTCCAAGGCTCTATAGGAGGAAAAGTTGGACCAAGATGGATAAAAATGTTGCTTTCTTCTTTCTCATTTTGCATCTACTTTGTCTTTTGGCCCCGTTCGATTTCAGTTGGAGTGGTTTTTGGCTTGCCATCGTAGTAGGCAATGCTACAGGTATGGCAATTAGTGTTTCGTTTCATAGAAATCTTGCACATCGAAGCTTCAAACTTCCCAAATGGCTTGAGTATTCGCTTGCTTATTGTGCATCTCATGCACTTCAGGGTGATCCAATTGATTGGGTGAGCACACACAGGTGCCATCATCGATATGTTGATACAGAACGAGACCCACATAGTCCTATATATGGATTTTGGTTTAGTCAAATAACATGGTTTTTCGATTCCTATAACTTGACTAGAAAAGTGTGCCCAAATTATTTCAATAACTTTCAAAAGGTAGAAAGAAATAGCTTTGTGTTGTTCTTGAAGCATGGGTATCCAGACAATGTCAACGACTTGGAGAGACAAGCTTTCTATAGGTTTATGCACAAAACATATATGCTTCATCCACTTGCTCTTTCAGCATTGTTGTATGCCATCGGAGGATTTCATTTTATTCTATGGGGAATGTGTGTGAGGTACATTCTGAGCTTGCATATAACATCTATGATAAATTCAGTATGTCATATATGGGGAAAACAACCATGGAATACTGGTGATTTGTCTCGAAACAATTGGTTGGTGGCTTTATTTACCTTTGGAGAAGGATGGCATAATAACCACCATGCTTTTGAGTATTCAGCAAGGCATGGTCATGAATGGTGGCAGATTGATTTTGGTTGGTACATTATTAAGTTTCTTCATGTCATTGGACTGGCAACTAATGTGAAATTACCTTCTCCAACCAAAAAGTTGGCAGCTTTGGACAAATCAAAACTCACCCTTCTTAAGCCTATGATTAATTAA

Protein sequence

MEESKQEAKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVGNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPTKKLAALDKSKLTLLKPMIN

Homology

[Close]

BLAST of Lag0002313 vs. NCBI nr
Match: XP_038899717.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 584.3 bits (1505), Expect = 6.4e-163
Identity = 266/327 (81.35%), Postives = 298/327 (91.13%), Query Frame = 0


Query: 2   EESKQEAKEMV-KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVV 61
           E SK+  KEMV KSPPRL+RRK WTK D+NVAF+FLILHLLC+LAPF FSW+  +LA+ +
Sbjct: 3   EASKEGPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLILHLLCILAPFYFSWTCLFLALGL 62

Query: 62  GNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 121
              TGM ISVS+HRNLAHRSFKLPKWLEYSLAY A+HA+QGDPIDWVSTHRCHHRYVD+E
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRYVDSE 122

Query: 122 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 181
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYFNNFQKVERN+F+ ++KHG+PDNV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGHPDNVTDLE 182

Query: 182 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQ 241
           +Q FYRF+HKTY LHPLALS LLYAIGG  FI+WGMCVR++ SLH+TSM+NSVCHIWGKQ
Sbjct: 183 KQRFYRFIHKTYWLHPLALSVLLYAIGGVPFIIWGMCVRFVFSLHVTSMVNSVCHIWGKQ 242

Query: 242 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 301
           PWNTGDLS+NNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFL VIGLAT
Sbjct: 243 PWNTGDLSKNNWMVAILTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGLAT 302

Query: 302 NVKLPSPTKKLAALDKSKLTLLKPMIN 328
           NVKLPS  +KLAAL+KSK T LKPMI+
Sbjct: 303 NVKLPSEKQKLAALNKSKPTFLKPMID 329

[Close]

BLAST of Lag0002313 vs. NCBI nr
Match: XP_008451258.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 576.6 bits (1485), Expect = 1.3e-160
Identity = 261/327 (79.82%), Postives = 296/327 (90.52%), Query Frame = 0


Query: 2   EESKQEAKEMV-KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVV 61
           E SK+E+KEMV  SP RL++RK WTK DKNVAF+FL+LHLLC+LAPF FSW+  +L++ +
Sbjct: 3   EASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSLGL 62

Query: 62  GNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 121
            N TGM ISVS+HRNLAHRSFKLPKWLEYSLAY A+HA+QGDPIDWVSTHRCHHR+VD+E
Sbjct: 63  ANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122

Query: 122 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 181
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYFNNFQKVERN+F+ ++KHGY DNV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTDLE 182

Query: 182 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQ 241
           +Q FYRF+HKTY LHPLALS LLYAIGG  FI+WGMCVR+++SLH TSMINSVCHIWG Q
Sbjct: 183 KQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWGNQ 242

Query: 242 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 301
           PWNTGDLS+NNW+VA+FTFGEGWHNNHHAFEYSARHGHEWWQID+GWYIIKFL VIG AT
Sbjct: 243 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGFAT 302

Query: 302 NVKLPSPTKKLAALDKSKLTLLKPMIN 328
           NVKLPS  +K AAL+KSK T LKPMI+
Sbjct: 303 NVKLPSEKQKWAALNKSKPTFLKPMID 329

[Close]

BLAST of Lag0002313 vs. NCBI nr
Match: XP_023549408.1 (delta-9 acyl-lipid desaturase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571.2 bits (1471), Expect = 5.6e-159
Identity = 254/315 (80.63%), Postives = 286/315 (90.79%), Query Frame = 0


Query: 4   SKQEAKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVGNA 63
           +K+    MV+SPP L+ RK WTK D+NVA +FL+LHLLC+LAPF F+W+ F+L I++GN 
Sbjct: 17  TKKHPNNMVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIILGNL 76

Query: 64  TGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDP 123
           TGMAISVS+HRNLAHR+FKLPKWLEYSLAYCA+HA+QGDPIDWVSTHRCHHRYVDTERDP
Sbjct: 77  TGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTERDP 136

Query: 124 HSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQA 183
           HSPIYGFWFSQ+TWFFDSYNLTRKVCPNYF NF+KVERN F+ F+KHGYPDNV DLE+Q 
Sbjct: 137 HSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLEKQW 196

Query: 184 FYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWN 243
           FYRFMHKTY LHPLAL+ LLYA+GG  +++WGMCVR+++SLH+TSMINSVCHIWGKQPWN
Sbjct: 197 FYRFMHKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVVSLHVTSMINSVCHIWGKQPWN 256

Query: 244 TGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVK 303
           TGDLSRNNWLVAL TFGEGWHNNHHAFEYSARHGH WWQIDFGWYIIKFL VIGLAT VK
Sbjct: 257 TGDLSRNNWLVALITFGEGWHNNHHAFEYSARHGHVWWQIDFGWYIIKFLQVIGLATCVK 316

Query: 304 LPSPTKKLAALDKSK 319
           LPS T  LAAL+KSK
Sbjct: 317 LPSQTHNLAALNKSK 331

[Close]

BLAST of Lag0002313 vs. NCBI nr
Match: XP_022953867.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 568.5 bits (1464), Expect = 3.6e-158
Identity = 254/318 (79.87%), Postives = 288/318 (90.57%), Query Frame = 0


Query: 1   MEESKQEAKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVV 60
           ME SKQ    MV+SPP L+ RK WTK D+NVA +FL+LHLLC+LAPF F+W+ F+L I++
Sbjct: 1   MEASKQH-PNMVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTSFFLTIIL 60

Query: 61  GNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 120
            N TGMAISVS+HRNLAHR+FKLPKWLEYSLAYCA+HA+QGDPIDWVSTHRCHHRYVDTE
Sbjct: 61  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 120

Query: 121 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 180
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYF NF+KVERN F+ F+KHGYPDNV DLE
Sbjct: 121 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 180

Query: 181 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQ 240
           +Q FYRFM KTY LHPLAL+ LLYA+GG  +++WGMCVR++LSLHITSM+NSVCHIWG Q
Sbjct: 181 KQWFYRFMRKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVLSLHITSMVNSVCHIWGTQ 240

Query: 241 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 300
           PWNTGDLSRNNW+VAL TFGEGWHNNHHAFEYSARHGHEWW+ID+GWY+IKFL VIGLAT
Sbjct: 241 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWEIDYGWYVIKFLQVIGLAT 300

Query: 301 NVKLPSPTKKLAALDKSK 319
            VKLPS T+KLAAL+KSK
Sbjct: 301 CVKLPSQTQKLAALNKSK 317

[Close]

BLAST of Lag0002313 vs. NCBI nr
Match: XP_022992040.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 565.1 bits (1455), Expect = 4.0e-157
Identity = 254/318 (79.87%), Postives = 285/318 (89.62%), Query Frame = 0


Query: 1   MEESKQEAKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVV 60
           ME SKQ    MV+SPP L+ RK WTK D+NVA +FL+LHLLC+LAPF F+W+ F+L I++
Sbjct: 1   MEASKQH-PNMVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIIL 60

Query: 61  GNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 120
            N TGMAISVS+HRNLAHR+FKLPKWLEYSLAYCA+HA+QGDPIDWVSTHRCHHRYVDTE
Sbjct: 61  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 120

Query: 121 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 180
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYF NF+KVERN F+ F+KHGYPDNV DLE
Sbjct: 121 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 180

Query: 181 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQ 240
           +Q FYRFM KTY LHPLAL+ LLYA GG  +++WGMCVR++LSLH+TSM+NSVCHIWG Q
Sbjct: 181 KQWFYRFMRKTYWLHPLALAGLLYAAGGIPYVIWGMCVRFVLSLHVTSMVNSVCHIWGNQ 240

Query: 241 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 300
           PWNTGDLSRNNW+VAL TFGEGWHNNHHAFEYSARHGHEWWQID+GWYIIKFL VIGLAT
Sbjct: 241 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 300

Query: 301 NVKLPSPTKKLAALDKSK 319
            VKLPS T  LAAL+KSK
Sbjct: 301 CVKLPSQTHNLAALNKSK 317

[Close]

BLAST of Lag0002313 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 359.0 bits (920), Expect = 5.7e-98
Identity = 163/290 (56.21%), Postives = 204/290 (70.34%), Query Frame = 0


Query: 22  KSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVGNATG-MAISVSFHRNLAHRS 81
           + W  MD       L +HLL LLAPF F+W    +A  +   TG + I++SFHRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 82  FKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWFFD 141
           FKLPKWLEY  AYC + ALQG+PIDWVSTHR HH++ D++RDPHSP+ GFWFS + W FD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 142 SYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLALS 201
           +  +T++                       G P+NV DLE+Q FYRF+  TY+LHPLAL+
Sbjct: 216 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 275

Query: 202 ALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFTFG 261
             LYA+GGF FI+WGM VR +   HIT ++NS CH+WGKQ WNTGDLS+NNW VA   FG
Sbjct: 276 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFG 335

Query: 262 EGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPTKK 311
           EGWHNNHHAFE+SARHG EWWQ+D  WY++KFL  IGLAT+VKLPS  +K
Sbjct: 336 EGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQK 363

[Close]

BLAST of Lag0002313 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 4.8e-89
Identity = 155/290 (53.45%), Postives = 187/290 (64.48%), Query Frame = 0


Query: 22  KSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVGNATGM-AISVSFHRNLAHRS 81
           + W   D     F   LH+LCL AP  FSW  FW+   +    G+   ++SFHRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 82  FKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWFFD 141
           FKLPK+LEY  AY   HALQGDP+ WVSTHR HH+Y DT  DPHSPI GFWF  I W FD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 142 SYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLALS 201
           S  +  K C  Y                     +N  DL +Q++YRF+ +T++ H    +
Sbjct: 158 SKYIIEK-CGRY---------------------ENAGDLMKQSYYRFLERTFVYHVYLQA 217

Query: 202 ALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFTFG 261
           ALLY  GGF FI+WGM VR IL  H++ ++NSVCH WG +PWNTGDLS NNW +A+ T G
Sbjct: 218 ALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSG 277

Query: 262 EGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPTKK 311
           EGWHNNHHAFEYSARHG EWWQID  WYIIK L  +GLAT++K+PS   K
Sbjct: 278 EGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHK 305

[Close]

BLAST of Lag0002313 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.4e-88
Identity = 162/299 (54.18%), Postives = 193/299 (64.55%), Query Frame = 0


Query: 21  RKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLA-IVVGNATG-MAISVSFHRNLAH 80
           R+ W   D   A   L+LH L  +APF FSW  FW++ I++G A+G + I++ FHR L H
Sbjct: 78  RRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGITLCFHRCLTH 137

Query: 81  RSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWF 140
             FKLPK +EY  AYC S ALQGDP++WVS HR HH++VDTERD HSP  GFWF  I W 
Sbjct: 138 GGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGFWFCHIGWV 197

Query: 141 FDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLA 200
            D      K                     + G  +NVNDL++QAFYRF+ KTYM H LA
Sbjct: 198 LDKDLFVEK---------------------RGGRRNNVNDLKKQAFYRFLQKTYMYHQLA 257

Query: 201 LSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFT 260
           L ALLY +GGF +I+WGM  R +   H T  INSVCH WG +PWNTGDLS NN  VAL  
Sbjct: 258 LIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGDLSTNNMFVALCA 317

Query: 261 FGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPTKKLAALDKS 318
           FGEGWHNNHHAFE SARHG EWW+ID  WY+I+ L  IGLATNVKLP+  +K     KS
Sbjct: 318 FGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLPTEAQKQKLKAKS 355

[Close]

BLAST of Lag0002313 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.4e-88
Identity = 152/314 (48.41%), Postives = 201/314 (64.01%), Query Frame = 0


Query: 3   ESKQEAKEMVKSPPRLYRRK------SWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWL 62
           E ++  K+M      + R+K       W ++D   AF  L +H LCLLAPF+F+W    +
Sbjct: 7   EKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRV 66

Query: 63  AIVVGNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRY 122
           A++V    G+ I+VS+HRNLAHRSFK+PKWLEY  AYC   A+QGDPIDWVSTHR HH++
Sbjct: 67  ALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQF 126

Query: 123 VDTERDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNV 182
            D++RDPHSP  GFWFS + W FD+  L  K                       G   NV
Sbjct: 127 TDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNV 186

Query: 183 NDLERQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHI 242
            DL+RQ +Y+F+ +T + H L    LLY  GG  F+ WGM +   +  H+T +INS+CH+
Sbjct: 187 EDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHV 246

Query: 243 WGKQPWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVI 302
           WG + W T D SRN W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++FL +I
Sbjct: 247 WGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEII 298

Query: 303 GLATNVKLPSPTKK 311
           GLAT+VKLPS +++
Sbjct: 307 GLATDVKLPSESQR 298

[Close]

BLAST of Lag0002313 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 322.8 bits (826), Expect = 4.5e-87
Identity = 148/293 (50.51%), Postives = 191/293 (65.19%), Query Frame = 0


Query: 17  RLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVGNATGMAISVSFHRNL 76
           R +  +SWT  D +    F+  HLL LLAPF FSW  FW+   +    G+ I++S+HRNL
Sbjct: 84  RSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICITLSYHRNL 143

Query: 77  AHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQIT 136
           +HRSF LPKWLEY  AY    A QGDPI+WVS HR HH++ +T+RDPHSP  GFWFS + 
Sbjct: 144 SHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMA 203

Query: 137 WFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHP 196
           W FD+ ++                          G  +NV+DL RQ FYRF+ +T +LH 
Sbjct: 204 WIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQRTVLLHM 263

Query: 197 LALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVAL 256
           +A S L Y  GG   ++WG+ +   + LH+T ++NSVCHIWG + WNT D S+NNW VA+
Sbjct: 264 MAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAI 323

Query: 257 FTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPTK 310
            T GEGWHNNHHAFE+SARHG EWWQ+D  W +I+FL  IGLATNVKLP+ T+
Sbjct: 324 LTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQ 354

[Close]

BLAST of Lag0002313 vs. ExPASy TrEMBL
Match: A0A1S3BS49 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492608 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.4e-161
Identity = 261/327 (79.82%), Postives = 296/327 (90.52%), Query Frame = 0


Query: 2   EESKQEAKEMV-KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVV 61
           E SK+E+KEMV  SP RL++RK WTK DKNVAF+FL+LHLLC+LAPF FSW+  +L++ +
Sbjct: 3   EASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSLGL 62

Query: 62  GNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 121
            N TGM ISVS+HRNLAHRSFKLPKWLEYSLAY A+HA+QGDPIDWVSTHRCHHR+VD+E
Sbjct: 63  ANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122

Query: 122 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 181
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYFNNFQKVERN+F+ ++KHGY DNV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTDLE 182

Query: 182 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQ 241
           +Q FYRF+HKTY LHPLALS LLYAIGG  FI+WGMCVR+++SLH TSMINSVCHIWG Q
Sbjct: 183 KQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWGNQ 242

Query: 242 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 301
           PWNTGDLS+NNW+VA+FTFGEGWHNNHHAFEYSARHGHEWWQID+GWYIIKFL VIG AT
Sbjct: 243 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGFAT 302

Query: 302 NVKLPSPTKKLAALDKSKLTLLKPMIN 328
           NVKLPS  +K AAL+KSK T LKPMI+
Sbjct: 303 NVKLPSEKQKWAALNKSKPTFLKPMID 329

[Close]

BLAST of Lag0002313 vs. ExPASy TrEMBL
Match: A0A6J1GPG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456275 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.8e-158
Identity = 254/318 (79.87%), Postives = 288/318 (90.57%), Query Frame = 0


Query: 1   MEESKQEAKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVV 60
           ME SKQ    MV+SPP L+ RK WTK D+NVA +FL+LHLLC+LAPF F+W+ F+L I++
Sbjct: 1   MEASKQH-PNMVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTSFFLTIIL 60

Query: 61  GNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 120
            N TGMAISVS+HRNLAHR+FKLPKWLEYSLAYCA+HA+QGDPIDWVSTHRCHHRYVDTE
Sbjct: 61  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 120

Query: 121 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 180
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYF NF+KVERN F+ F+KHGYPDNV DLE
Sbjct: 121 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 180

Query: 181 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQ 240
           +Q FYRFM KTY LHPLAL+ LLYA+GG  +++WGMCVR++LSLHITSM+NSVCHIWG Q
Sbjct: 181 KQWFYRFMRKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVLSLHITSMVNSVCHIWGTQ 240

Query: 241 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 300
           PWNTGDLSRNNW+VAL TFGEGWHNNHHAFEYSARHGHEWW+ID+GWY+IKFL VIGLAT
Sbjct: 241 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWEIDYGWYVIKFLQVIGLAT 300

Query: 301 NVKLPSPTKKLAALDKSK 319
            VKLPS T+KLAAL+KSK
Sbjct: 301 CVKLPSQTQKLAALNKSK 317

[Close]

BLAST of Lag0002313 vs. ExPASy TrEMBL
Match: A0A0A0KAB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.7e-158
Identity = 258/327 (78.90%), Postives = 292/327 (89.30%), Query Frame = 0


Query: 2   EESKQEAKEMV-KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVV 61
           E SK++ KEMV KSPPRL+RRK WTK D+NVAF+FL LHLLC+LAPF FSW+   LA+ +
Sbjct: 3   EVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGL 62

Query: 62  GNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 121
              TGM ISVS+HRNLAHRSFKLPKWLEYSLAY A+HA+QGDPIDWVSTHRCHHR+VD+ 
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSA 122

Query: 122 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 181
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYFNN+QKVERN F+ ++KHGYPDNV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLE 182

Query: 182 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQ 241
           +Q FYRF+HKTY LHPLALS LLYAIGG  FI+WGMCVR++ SLH+TSM+NSVCHIWG Q
Sbjct: 183 KQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQ 242

Query: 242 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 301
           PWNTGDLS+NNW+VAL TFGEGWHNNHHAFEYSARHGHEWWQID+GWY+I+FL VIGLAT
Sbjct: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLAT 302

Query: 302 NVKLPSPTKKLAALDKSKLTLLKPMIN 328
           NVKLPS  +KLAAL+KSK   LK MI+
Sbjct: 303 NVKLPSEKQKLAALNKSKPIFLKLMID 329

[Close]

BLAST of Lag0002313 vs. ExPASy TrEMBL
Match: A0A6J1JUL6 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488510 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.9e-157
Identity = 254/318 (79.87%), Postives = 285/318 (89.62%), Query Frame = 0


Query: 1   MEESKQEAKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVV 60
           ME SKQ    MV+SPP L+ RK WTK D+NVA +FL+LHLLC+LAPF F+W+ F+L I++
Sbjct: 1   MEASKQH-PNMVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIIL 60

Query: 61  GNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 120
            N TGMAISVS+HRNLAHR+FKLPKWLEYSLAYCA+HA+QGDPIDWVSTHRCHHRYVDTE
Sbjct: 61  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 120

Query: 121 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 180
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYF NF+KVERN F+ F+KHGYPDNV DLE
Sbjct: 121 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 180

Query: 181 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQ 240
           +Q FYRFM KTY LHPLAL+ LLYA GG  +++WGMCVR++LSLH+TSM+NSVCHIWG Q
Sbjct: 181 KQWFYRFMRKTYWLHPLALAGLLYAAGGIPYVIWGMCVRFVLSLHVTSMVNSVCHIWGNQ 240

Query: 241 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 300
           PWNTGDLSRNNW+VAL TFGEGWHNNHHAFEYSARHGHEWWQID+GWYIIKFL VIGLAT
Sbjct: 241 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 300

Query: 301 NVKLPSPTKKLAALDKSK 319
            VKLPS T  LAAL+KSK
Sbjct: 301 CVKLPSQTHNLAALNKSK 317

[Close]

BLAST of Lag0002313 vs. ExPASy TrEMBL
Match: A0A6J1GMY0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111455910 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.0e-154
Identity = 245/308 (79.55%), Postives = 280/308 (90.91%), Query Frame = 0


Query: 11  MVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVGNATGMAISV 70
           M+KSPP L+ RK WTK+D+N A + L+LHLLC+LAPF F+W+ F+L I++GN TGMAISV
Sbjct: 1   MMKSPPMLFTRKRWTKLDRNNASYILLLHLLCILAPFYFTWTCFFLTIILGNLTGMAISV 60

Query: 71  SFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGF 130
           S+HRNLAHR+FKLPKWLEY LAYCA+HA+QGDPI+WVSTHRCHHRYVDTERDPHSPIYGF
Sbjct: 61  SYHRNLAHRAFKLPKWLEYFLAYCAAHAIQGDPINWVSTHRCHHRYVDTERDPHSPIYGF 120

Query: 131 WFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHK 190
           WFSQ+TWFFDSYNLTRKVCP YFNNF+KVERN F+ F+K+  PDNV DLE+Q FYRFMHK
Sbjct: 121 WFSQMTWFFDSYNLTRKVCPKYFNNFEKVERNKFMFFIKYANPDNVRDLEKQWFYRFMHK 180

Query: 191 TYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWNTGDLSRN 250
           TY LHPLAL+ LL+AIGG  +++WGMCVR++LSLH+TSM+NSVCHIWGKQPWNTGDLS+N
Sbjct: 181 TYWLHPLALAVLLFAIGGVPYVIWGMCVRFVLSLHVTSMVNSVCHIWGKQPWNTGDLSKN 240

Query: 251 NWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPTKK 310
           NW+VAL TFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFL VIGLAT VKLPS T  
Sbjct: 241 NWMVALITFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGLATCVKLPSQTHN 300

Query: 311 LAALDKSK 319
           LAAL+KSK
Sbjct: 301 LAALNKSK 308

[Close]

BLAST of Lag0002313 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 359.0 bits (920), Expect = 4.1e-99
Identity = 163/290 (56.21%), Postives = 204/290 (70.34%), Query Frame = 0


Query: 22  KSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVGNATG-MAISVSFHRNLAHRS 81
           + W  MD       L +HLL LLAPF F+W    +A  +   TG + I++SFHRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 82  FKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWFFD 141
           FKLPKWLEY  AYC + ALQG+PIDWVSTHR HH++ D++RDPHSP+ GFWFS + W FD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 142 SYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLALS 201
           +  +T++                       G P+NV DLE+Q FYRF+  TY+LHPLAL+
Sbjct: 216 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 275

Query: 202 ALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFTFG 261
             LYA+GGF FI+WGM VR +   HIT ++NS CH+WGKQ WNTGDLS+NNW VA   FG
Sbjct: 276 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFG 335

Query: 262 EGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPTKK 311
           EGWHNNHHAFE+SARHG EWWQ+D  WY++KFL  IGLAT+VKLPS  +K
Sbjct: 336 EGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQK 363

[Close]

BLAST of Lag0002313 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 327.0 bits (837), Expect = 1.7e-89
Identity = 152/314 (48.41%), Postives = 201/314 (64.01%), Query Frame = 0


Query: 3   ESKQEAKEMVKSPPRLYRRK------SWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWL 62
           E ++  K+M      + R+K       W ++D   AF  L +H LCLLAPF+F+W    +
Sbjct: 7   EKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRV 66

Query: 63  AIVVGNATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRY 122
           A++V    G+ I+VS+HRNLAHRSFK+PKWLEY  AYC   A+QGDPIDWVSTHR HH++
Sbjct: 67  ALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQF 126

Query: 123 VDTERDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNV 182
            D++RDPHSP  GFWFS + W FD+  L  K                       G   NV
Sbjct: 127 TDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNV 186

Query: 183 NDLERQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHI 242
            DL+RQ +Y+F+ +T + H L    LLY  GG  F+ WGM +   +  H+T +INS+CH+
Sbjct: 187 EDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHV 246

Query: 243 WGKQPWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVI 302
           WG + W T D SRN W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++FL +I
Sbjct: 247 WGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEII 298

Query: 303 GLATNVKLPSPTKK 311
           GLAT+VKLPS +++
Sbjct: 307 GLATDVKLPSESQR 298

[Close]

BLAST of Lag0002313 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 322.8 bits (826), Expect = 3.2e-88
Identity = 148/293 (50.51%), Postives = 191/293 (65.19%), Query Frame = 0


Query: 17  RLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVGNATGMAISVSFHRNL 76
           R +  +SWT  D +    F+  HLL LLAPF FSW  FW+   +    G+ I++S+HRNL
Sbjct: 84  RSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICITLSYHRNL 143

Query: 77  AHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQIT 136
           +HRSF LPKWLEY  AY    A QGDPI+WVS HR HH++ +T+RDPHSP  GFWFS + 
Sbjct: 144 SHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMA 203

Query: 137 WFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHP 196
           W FD+ ++                          G  +NV+DL RQ FYRF+ +T +LH 
Sbjct: 204 WIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQRTVLLHM 263

Query: 197 LALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVAL 256
           +A S L Y  GG   ++WG+ +   + LH+T ++NSVCHIWG + WNT D S+NNW VA+
Sbjct: 264 MAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAI 323

Query: 257 FTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPTK 310
            T GEGWHNNHHAFE+SARHG EWWQ+D  W +I+FL  IGLATNVKLP+ T+
Sbjct: 324 LTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQ 354

[Close]

BLAST of Lag0002313 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 319.7 bits (818), Expect = 2.7e-87
Identity = 152/307 (49.51%), Postives = 194/307 (63.19%), Query Frame = 0


Query: 4   SKQEAKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVGNA 63
           S   A E  K    ++  + W ++D         +H L LLAPF F+WS  W+  +    
Sbjct: 16  STPAAVEEKKKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTI 75

Query: 64  TGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDP 123
            G+ I+VS+HRNLAHRSFK+PKWLEY LAYCA  A+QGDPIDWVSTHR HH++ D+ERDP
Sbjct: 76  GGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDP 135

Query: 124 HSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQA 183
           HSP  GFWFS + W +DS  L  K                       G   NV DL+RQ 
Sbjct: 136 HSPKEGFWFSHLLWIYDSAYLVSKC----------------------GRRANVEDLKRQW 195

Query: 184 FYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQPWN 243
           FYRF+ KT + H L L   L+ +GG  F+ WGM V   L +H+T +INS+CHIWG + W 
Sbjct: 196 FYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWK 255

Query: 244 TGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVK 303
           T D SRN W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++F  +IGLAT+VK
Sbjct: 256 TNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVK 300

Query: 304 LPSPTKK 311
           +P+  ++
Sbjct: 316 VPTEAQR 300

[Close]

BLAST of Lag0002313 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 308.1 bits (788), Expect = 8.2e-84
Identity = 151/309 (48.87%), Postives = 196/309 (63.43%), Query Frame = 0


Query: 2   EESKQEAKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFWLAIVVG 61
           ++S  ++K  V+   R +  + WT++D         +HLLCLLAPF+++W  F  A +VG
Sbjct: 7   DDSSSQSK-AVRKEKRAFLFRKWTRVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVG 66

Query: 62  NATGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTER 121
            +T ++I+ S+HRNL HRSFKLPKWLEY  AY A  ALQG PIDWVSTHR HH++ D++R
Sbjct: 67  ISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDR 126

Query: 122 DPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLER 181
           DPHSPI GFWFS + W FD+ +  R+ C                     G  DNV DL++
Sbjct: 127 DPHSPIEGFWFSHVFWIFDT-SYIREKC---------------------GGRDNVMDLKQ 186

Query: 182 QAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYILSLHITSMINSVCHIWGKQP 241
           Q FYRF+  T  LH L    L+Y  GG  ++ W + V   +  H T +INS CHIWG + 
Sbjct: 187 QWFYRFLQNTIGLHILTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGSRA 246

Query: 242 WNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATN 301
           WNT D SRN W +  FT GE WHNNHHAFE SARHG EW+Q+D  WY+I F  V+GLAT+
Sbjct: 247 WNTKDTSRNIWWLGPFTMGESWHNNHHAFEASARHGLEWYQVDLTWYLIWFFQVLGLATD 292

Query: 302 VKLPSPTKK 311
           VKLP+  +K
Sbjct: 307 VKLPTDAQK 292

The following BLAST results are available for this feature:

BLAST of Lag0002313 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899717.1	6.4e-163	81.35	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B...	[more]
XP_008451258.1	1.3e-160	79.82	PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas...	[more]
XP_023549408.1	5.6e-159	80.63	delta-9 acyl-lipid desaturase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022953867.1	3.6e-158	79.87	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]
XP_022992040.1	4.0e-157	79.87	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]

BLAST of Lag0002313 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	5.7e-98	56.21	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
P0DOW2	4.8e-89	53.45	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
Q9FV68	1.4e-88	54.18	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1	[more]
O65797	2.4e-88	48.41	Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=...	[more]
Q9LVZ3	4.5e-87	50.51	Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Lag0002313 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BS49	6.4e-161	79.82	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GPG5	1.8e-158	79.87	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A0A0KAB1	6.7e-158	78.90	FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G3763...	[more]
A0A6J1JUL6	1.9e-157	79.87	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GMY0	2.0e-154	79.55	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

BLAST of Lag0002313 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	4.1e-99	56.21	fatty acid desaturase 5	[more]
AT1G06080.1	1.7e-89	48.41	delta 9 desaturase 1	[more]
AT3G15870.1	3.2e-88	50.51	Fatty acid desaturase family protein	[more]
AT2G31360.1	2.7e-87	49.51	16:0delta9 desaturase 2	[more]
AT1G06120.1	8.2e-84	48.87	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 73..93 score: 37.09 coord: 110..139 score: 46.32
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 12..311
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 50..303 e-value: 1.17514E-69 score: 212.801
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 50..287 e-value: 1.2E-15 score: 58.0
None	No IPR available	PANTHER	PTHR11351:SF87	FATTY ACID DESATURASE, TYPE 1, CORE-RELATED	coord: 12..311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002313.1	Lag0002313.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water