Lag0002310 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002310
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein KINESIN LIGHT CHAIN-RELATED 1
Locationchr4: 41546340 .. 41549379 (+)
RNA-Seq ExpressionLag0002310
SyntenyLag0002310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAGAAACGCTTCAATTTCACTTCTTTCCCACACCAATCGTCTCAATCTCCGCTCACTTTCATCTCTCATTTCCCCAAATTCCATTTCCAATCCTTCCCCTTCATGTTCCTCCCCTGTTTCAACCCCACATCTCAAGCCCTGCACCAAAAACCATGGCTTGTTCTTCAAATTCACCCGATTTCAAGCCAACCCATCTCGGAATTTGGACACCCTTGTGGAGAAACGCCCTCAGATTTCCTCCAGACAGCGAAGAGTCAAAGAGAAATCCGAGCTCGAGGAGGCCTTTGAGTCTGCGGACACTGTTGATGAAATGGTCAAGGCGTTTAAGGACATGGAATCCGCTTTTGAAGAGAGGGATCTTGGATTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAAGAAGGTGAGGATCCTGAAAAGGCTCTGGTGTATGCTAGTAGAGCTTTGAATGCTTTGGATAAAGATGATAATGGGAAACCTTCTTTACTTATTGCTATGACTTTGCAATTGATGGGTTCTGTTAATTATAGCTTGAAAAAGTTTAGTGATAGTTTGGGGTATTTGAATAGGGCCAACAGGGTTCTGGGTCAGTTAGAGGGGAAGGGTTTTAGTGCTGAGGATGTTAGGCCAGTGCTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAGACTGCAATGGGAAGGAGAGAAGAAGCTATTGGTAATCTTAGGAATTGTTTAGAGATCAAGGAGTTGACCTTGGAGAAGGATAGTAGAGAATTGGGGAAGGCGAACCGTGATTTGGCCGAAGCTTATGTTGCTATTTTGAACTTCAAGGATGCCCTTCCCTTTTGTATGAAGGCATTGGAGATACATAAGAAGCAGTTGGGACATAATTCAGTTGAAGTTGCTCATGATAGGAGGCTTCTTGGGGTGATTTATAGTGGGTTGGAAGAACATCAGAAAGCATTGGAGCAGAATGAATTGTCTCAGAAGGTTTTGAAGAACTGGGGCCTTAATTCCGATTTGCTTCGTGCGGAAATTGATGCTGCTAATATGCAAATTGCGCTAGGTAGGTATGATGAGGCCATTAATACTCTCAAGGATGTTGTTCAGCATACAGATAAAGACAGTGAAACTCGGGCAGTGGTGTTTATCTCAATGGGAAAGGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAAATTGCCAGTGGAATTTTTGACAAGAAAGAAGCAACTTCTCCTGTGGAAGCCGCTGAGGCGTATTCAGAAATATCAATGCAGTATGAGACAATGAATGAGTTCGAAACTGCAATTTCTTTATTAAAAAGATCTCTAGCTTTGCTTGAGAAGCTTCCACAAGAGCAGCACTCAGAAGGTAGTGTTTCAGCTAGATTAGGGTGGTTGCTACTGTTGACCGGTAAGGTTCAAGAAGCTATTCCATACTTGGAGGGTGCAGCAGAGACTTTGAGAGAGAGTTTTGGTTCCAAACATTTTGGAGTTGGATATATATACAATAATCTCGGGGCTGCATATTTGGAACTCGAGAGACCTCAATCGGCTGCACAGATGTTTGCTATGGCAAAGGACATTATGGACGTATCACTTGGCCCCCATCATGCAGATTCAATTGAAGCATGTCAGAATCTTTCAAAAGCATATGGTGCAATGGGAAGGTACGGTTACTTTCAAGCTTTACTTTCTTCTCCTCACTATTTATAATGTTTTACTTATTATCACTCTTTGAAATCCTTATTGGGGGAGTTGGGTTTTATGGATCATATTGAGACACGGAATCATATGAGTTAAGGAGATCAAGGGAATCATGGACGAGCTTTCAAAAAGTTTATTGTTTAGAATGGCAGAGTAGGAAACTGATCCATGTAATATGTCTTCTGACCTCGAAGCCGCTTAGTTCCTTTTGGAGATATTTTTAGCTTCCTTGTTTTCTTGTAAACCGAAAGTGGATAGCACTTAGCAGACATGTTTTGCCCCTCCTTAAGCTCTAGGGAGGAAGTTTTTCCCTTGTTAGCTTCCATACTCCCTCCTTTGGACTTCGGTGTGTTAATGACTTTTGTTATTACCCCTTAGGTATTATCTTGCTTGATTGGAACTCTTTTTTATAGTTTGGCTCCTTCTGGGGTTGTTTTTTTTATGCACTTTGTATTCTTTAATTTTTTCTCAATGAAAGCTCGATTTCAAATTAAAAAAATGTTTTTAATGCTCGGCCTATTCTGCTAATGCTTAGCCATGATTGTATTTGTACGGTTGTGGAAAATTTGAGGTTAATTAGATTCACAAAAATGACAAACAGACTTTGAGAGGATTCAAACCAATAATTTGACTTACCAAACTAATGGGTTGTTCATGATGGTGAAGTGAGGGTCTTTGAATTCAATCATGTGATCTTGGAATGGAATTTCCTTCAAGAGTCACTTGACAGAAACGATAAAAATATCTCACTAGTATTTTGAACAGAAATTATTATAGGGCAGATAGCTTTTTGTTTGGTCTAGATTCGAAAATAAAAATGAAAATGATGAATATTGAGGAGTCTAGCTATGATATTACCAAGTCGTCTTTGGTGATGATTGCTTGTGGAACTGACTTAATACAGTGAGTCGAACGTGCGGGTTGAATTGATTTTGAATTGCTTTTTAACTGTCATGAAGGTATTAGAACCATCTTCAATCCTGCGTAAAGTAACCTTTATTGTCTGCACGCATGGATCATTAGAGAAACTCAGCACAGCAGCAAGTGCCGTTCTTGAACTCATTAATTTACTCCTTTTTCTGTGTGTTGGTGCAGTTATGCCCTTGCAATTGAATTCCAAGAGCAGGTGGTTGATGCTTGGGAAAGCCATGGCTCAACTGCAGAAGATGAACTTAGAGAAGCCCAACGAATGCTCCAACAATTAAAGAGGAAAGCACATGGTACATCCAAAATCGAGCTCTCGACAAAGGCCTTGCCCCTACCTCATTCCCCAACGTCTTCCAAAACTTTGCAGACCGATATTCACAGCAGTCCGAATCATCTATGA

mRNA sequence

ATGAGAAGAAACGCTTCAATTTCACTTCTTTCCCACACCAATCGTCTCAATCTCCGCTCACTTTCATCTCTCATTTCCCCAAATTCCATTTCCAATCCTTCCCCTTCATGTTCCTCCCCTGTTTCAACCCCACATCTCAAGCCCTGCACCAAAAACCATGGCTTGTTCTTCAAATTCACCCGATTTCAAGCCAACCCATCTCGGAATTTGGACACCCTTGTGGAGAAACGCCCTCAGATTTCCTCCAGACAGCGAAGAGTCAAAGAGAAATCCGAGCTCGAGGAGGCCTTTGAGTCTGCGGACACTGTTGATGAAATGGTCAAGGCGTTTAAGGACATGGAATCCGCTTTTGAAGAGAGGGATCTTGGATTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAAGAAGGTGAGGATCCTGAAAAGGCTCTGGTGTATGCTAGTAGAGCTTTGAATGCTTTGGATAAAGATGATAATGGGAAACCTTCTTTACTTATTGCTATGACTTTGCAATTGATGGGTTCTGTTAATTATAGCTTGAAAAAGTTTAGTGATAGTTTGGGGTATTTGAATAGGGCCAACAGGGTTCTGGGTCAGTTAGAGGGGAAGGGTTTTAGTGCTGAGGATGTTAGGCCAGTGCTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAGACTGCAATGGGAAGGAGAGAAGAAGCTATTGGTAATCTTAGGAATTGTTTAGAGATCAAGGAGTTGACCTTGGAGAAGGATAGTAGAGAATTGGGGAAGGCGAACCGTGATTTGGCCGAAGCTTATGTTGCTATTTTGAACTTCAAGGATGCCCTTCCCTTTTGTATGAAGGCATTGGAGATACATAAGAAGCAGTTGGGACATAATTCAGTTGAAGTTGCTCATGATAGGAGGCTTCTTGGGGTGATTTATAGTGGGTTGGAAGAACATCAGAAAGCATTGGAGCAGAATGAATTGTCTCAGAAGGTTTTGAAGAACTGGGGCCTTAATTCCGATTTGCTTCGTGCGGAAATTGATGCTGCTAATATGCAAATTGCGCTAGGTAGGTATGATGAGGCCATTAATACTCTCAAGGATGTTGTTCAGCATACAGATAAAGACAGTGAAACTCGGGCAGTGGTGTTTATCTCAATGGGAAAGGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAAATTGCCAGTGGAATTTTTGACAAGAAAGAAGCAACTTCTCCTGTGGAAGCCGCTGAGGCGTATTCAGAAATATCAATGCAGTATGAGACAATGAATGAGTTCGAAACTGCAATTTCTTTATTAAAAAGATCTCTAGCTTTGCTTGAGAAGCTTCCACAAGAGCAGCACTCAGAAGGTAGTGTTTCAGCTAGATTAGGGTGGTTGCTACTGTTGACCGGTAAGGTTCAAGAAGCTATTCCATACTTGGAGGGTGCAGCAGAGACTTTGAGAGAGAGTTTTGGTTCCAAACATTTTGGAGTTGGATATATATACAATAATCTCGGGGCTGCATATTTGGAACTCGAGAGACCTCAATCGGCTGCACAGATGTTTGCTATGGCAAAGGACATTATGGACGTATCACTTGGCCCCCATCATGCAGATTCAATTGAAGCATGTCAGAATCTTTCAAAAGCATATGGTGCAATGGGAAGTTATGCCCTTGCAATTGAATTCCAAGAGCAGGTGGTTGATGCTTGGGAAAGCCATGGCTCAACTGCAGAAGATGAACTTAGAGAAGCCCAACGAATGCTCCAACAATTAAAGAGGAAAGCACATGGTACATCCAAAATCGAGCTCTCGACAAAGGCCTTGCCCCTACCTCATTCCCCAACGTCTTCCAAAACTTTGCAGACCGATATTCACAGCAGTCCGAATCATCTATGA

Coding sequence (CDS)

ATGAGAAGAAACGCTTCAATTTCACTTCTTTCCCACACCAATCGTCTCAATCTCCGCTCACTTTCATCTCTCATTTCCCCAAATTCCATTTCCAATCCTTCCCCTTCATGTTCCTCCCCTGTTTCAACCCCACATCTCAAGCCCTGCACCAAAAACCATGGCTTGTTCTTCAAATTCACCCGATTTCAAGCCAACCCATCTCGGAATTTGGACACCCTTGTGGAGAAACGCCCTCAGATTTCCTCCAGACAGCGAAGAGTCAAAGAGAAATCCGAGCTCGAGGAGGCCTTTGAGTCTGCGGACACTGTTGATGAAATGGTCAAGGCGTTTAAGGACATGGAATCCGCTTTTGAAGAGAGGGATCTTGGATTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAAGAAGGTGAGGATCCTGAAAAGGCTCTGGTGTATGCTAGTAGAGCTTTGAATGCTTTGGATAAAGATGATAATGGGAAACCTTCTTTACTTATTGCTATGACTTTGCAATTGATGGGTTCTGTTAATTATAGCTTGAAAAAGTTTAGTGATAGTTTGGGGTATTTGAATAGGGCCAACAGGGTTCTGGGTCAGTTAGAGGGGAAGGGTTTTAGTGCTGAGGATGTTAGGCCAGTGCTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAGACTGCAATGGGAAGGAGAGAAGAAGCTATTGGTAATCTTAGGAATTGTTTAGAGATCAAGGAGTTGACCTTGGAGAAGGATAGTAGAGAATTGGGGAAGGCGAACCGTGATTTGGCCGAAGCTTATGTTGCTATTTTGAACTTCAAGGATGCCCTTCCCTTTTGTATGAAGGCATTGGAGATACATAAGAAGCAGTTGGGACATAATTCAGTTGAAGTTGCTCATGATAGGAGGCTTCTTGGGGTGATTTATAGTGGGTTGGAAGAACATCAGAAAGCATTGGAGCAGAATGAATTGTCTCAGAAGGTTTTGAAGAACTGGGGCCTTAATTCCGATTTGCTTCGTGCGGAAATTGATGCTGCTAATATGCAAATTGCGCTAGGTAGGTATGATGAGGCCATTAATACTCTCAAGGATGTTGTTCAGCATACAGATAAAGACAGTGAAACTCGGGCAGTGGTGTTTATCTCAATGGGAAAGGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAAATTGCCAGTGGAATTTTTGACAAGAAAGAAGCAACTTCTCCTGTGGAAGCCGCTGAGGCGTATTCAGAAATATCAATGCAGTATGAGACAATGAATGAGTTCGAAACTGCAATTTCTTTATTAAAAAGATCTCTAGCTTTGCTTGAGAAGCTTCCACAAGAGCAGCACTCAGAAGGTAGTGTTTCAGCTAGATTAGGGTGGTTGCTACTGTTGACCGGTAAGGTTCAAGAAGCTATTCCATACTTGGAGGGTGCAGCAGAGACTTTGAGAGAGAGTTTTGGTTCCAAACATTTTGGAGTTGGATATATATACAATAATCTCGGGGCTGCATATTTGGAACTCGAGAGACCTCAATCGGCTGCACAGATGTTTGCTATGGCAAAGGACATTATGGACGTATCACTTGGCCCCCATCATGCAGATTCAATTGAAGCATGTCAGAATCTTTCAAAAGCATATGGTGCAATGGGAAGTTATGCCCTTGCAATTGAATTCCAAGAGCAGGTGGTTGATGCTTGGGAAAGCCATGGCTCAACTGCAGAAGATGAACTTAGAGAAGCCCAACGAATGCTCCAACAATTAAAGAGGAAAGCACATGGTACATCCAAAATCGAGCTCTCGACAAAGGCCTTGCCCCTACCTCATTCCCCAACGTCTTCCAAAACTTTGCAGACCGATATTCACAGCAGTCCGAATCATCTATGA

Protein sequence

MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFTRFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEERDLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSLKKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRNCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAHGTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNHL
Homology
BLAST of Lag0002310 vs. NCBI nr
Match: XP_038899312.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 588/628 (93.63%), Postives = 607/628 (96.66%), Query Frame = 0

Query: 1   MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFT 60
           MRRNASISLLSH+NRLNLRSLSS IS NSISNPSPS SSPVSTP+LKPCTKNHGLFFKFT
Sbjct: 1   MRRNASISLLSHSNRLNLRSLSSFISSNSISNPSPSNSSPVSTPNLKPCTKNHGLFFKFT 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNLDTLVEKRPQISSRQR+VKEKSELEEAFESADTVDEMVKAFKDMESAFEER
Sbjct: 61  RFQANPSRNLDTLVEKRPQISSRQRKVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSL 180
           DLGLASLKIGLKLDQEGEDPE  L+YA+RAL ALDKD NG PSL +AMTLQLMGSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPENILLYANRALKALDKDVNGNPSLPVAMTLQLMGSVNYSL 180

Query: 181 KKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 240
           K+FSDSLGYLNRANR+LGQL  KGFSAEDVRPVLHAVQLELANVKTAMGRREEA+ NLR 
Sbjct: 181 KRFSDSLGYLNRANRILGQLVEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELG ANRDLAEAYVA+LNFKDALPFCMKALEIHK QLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGNANRDLAEAYVAVLNFKDALPFCMKALEIHKMQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLL VIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRY+EAI+
Sbjct: 301 DRRLLAVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYNEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 420
           TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTD KRCLEIASGIFDK+EATSPVEAAE
Sbjct: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDPKRCLEIASGIFDKREATSPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYETMNEFETAISLLK+SLALLEKLP EQHSEGSVSARLGWLLLLTGKVQEAI
Sbjct: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPHEQHSEGSVSARLGWLLLLTGKVQEAI 480

Query: 481 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 600
           ADSIEAC+NLSKAYGAMGSY LAIEFQEQVVDAWESHGSTAEDELREAQ+ML+QLKRKAH
Sbjct: 541 ADSIEACRNLSKAYGAMGSYPLAIEFQEQVVDAWESHGSTAEDELREAQQMLKQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQTDI 629
           GTSKIELSTKALPLPHS TSSKTLQ D+
Sbjct: 601 GTSKIELSTKALPLPHSSTSSKTLQPDL 628

BLAST of Lag0002310 vs. NCBI nr
Match: XP_023549125.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 576/634 (90.85%), Postives = 602/634 (94.95%), Query Frame = 0

Query: 1   MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFT 60
           MRRNASISLL H+NR+NLRSLSSLISP  IS+PSPS SSPVS PHL PCTKNHGLFFKF 
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKFN 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQA+PSRNL+TLVEKRPQISSRQR+ KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQASPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK LVYA+RAL ALDKDDNGKPSL +AMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 240
           K+FSDSLGYLNRANR+LGQLE  GFSAE +RPVLHAVQLELANVKTAMGRREEA+ NLR 
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPV AAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVAAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAWE HGSTAEDELREAQRML+QLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSTAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNH 635
           G SKIELS KALPLPHS TSSKTLQ DI SS  H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 634

BLAST of Lag0002310 vs. NCBI nr
Match: XP_022954096.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita moschata])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 575/634 (90.69%), Postives = 601/634 (94.79%), Query Frame = 0

Query: 1   MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFT 60
           MRRNASISLL H+NR+NLRSLSSLISP  IS+PSPS SSPVS PHL PCTKNHGLFFK +
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKLS 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQR+ KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK LVYA+RAL ALDKDDNGKPSL +AMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 240
           K+FSDSLGYLNRANR+LGQLE  GFSAE +RPVLHAVQLELANVKTAMGRREEA+ NLR 
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELER QSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAWE HGS AEDELREAQRML+QLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNH 635
           G SKIELS KALPLPHS TSSKTLQ DI SS  H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 634

BLAST of Lag0002310 vs. NCBI nr
Match: KAG7014584.1 (Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 572/634 (90.22%), Postives = 601/634 (94.79%), Query Frame = 0

Query: 1   MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFT 60
           MRRNASISLL H+NR+NLRSLSSLISP  IS+PSPS SSPVS  HL PCTKNHGLFFKF+
Sbjct: 11  MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSASHLNPCTKNHGLFFKFS 70

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQR+ KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 71  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 130

Query: 121 DLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK LVYA+RAL ALDKDDNGKPSL +AMTLQL+GSVNYSL
Sbjct: 131 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 190

Query: 181 KKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 240
           K+FSDSLGYLNRANR+LGQLE  GFSAE +RPVLHAVQLELANVKTAMGRREEA+ +LR 
Sbjct: 191 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALASLRK 250

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSREL KANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 251 CLEIKELTLEKDSRELAKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 310

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGR+DEAI+
Sbjct: 311 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRFDEAIS 370

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA+SPVEAAE
Sbjct: 371 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEASSPVEAAE 430

Query: 421 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 431 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 490

Query: 481 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 491 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 550

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 600
           ADSIEACQNL+KAYG++ SYALAIEFQEQVVDAWE HGS AEDELREAQRML+QLKRKAH
Sbjct: 551 ADSIEACQNLAKAYGSLESYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 610

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNH 635
           G SKIELS KALPLPHS TSSKTLQ DI SS  H
Sbjct: 611 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 644

BLAST of Lag0002310 vs. NCBI nr
Match: KAG6576067.1 (Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 572/634 (90.22%), Postives = 601/634 (94.79%), Query Frame = 0

Query: 1   MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFT 60
           MRRNASISLL H+NR+NLRSLSSLISP  IS+PSPS SSPVS  HL PCTKNHGLFFKF+
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSASHLNPCTKNHGLFFKFS 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQR+ KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK LVYA+RAL ALDKDDNGKPSL +AMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 240
           K+FSDSLGYLNRANR+LGQLE  GFSAE +RPVLHAVQLELANVKTAMGRREEA+ +LR 
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALASLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSREL KANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELAKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGR+DEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRFDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA+SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEASSPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 600
           ADSIEACQNL+KAYG++ SYALAIEFQEQVVDAWE HGS AEDELREAQRML+QLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLESYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNH 635
           G SKIELS KALPLPHS TSSKTLQ DI SS  H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 634

BLAST of Lag0002310 vs. ExPASy Swiss-Prot
Match: O81629 (Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.8e-47
Identity = 163/581 (28.06%), Postives = 275/581 (47.33%), Query Frame = 0

Query: 36  SCSSPVSTPHLKPCTKNHGLFFKFTRFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEE 95
           S  +P  TP L+    +       T+  +NP R   T ++K P  SS   R K       
Sbjct: 9   SVKTPSDTPPLRVAVPD-------TQPLSNPPR---TPMKKTP--SSTPSRSKPSPNRST 68

Query: 96  AFESADTVDEMVKAFKDMES-AFEERDLGLASLKIGLKLDQEGEDPEKALVYASRALNAL 155
             + + TV     A  D++  + +  DLG   LK+       GE P KAL YA RA  + 
Sbjct: 69  GKKDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSF 128

Query: 156 DK-----------DDNGKPSLLIAMTLQLMGSVNYSLKKFSDSLGYLNRANRVLGQLEGK 215
           ++             +G P L +AM+L ++ ++  SL +F +++  L RA +V     G 
Sbjct: 129 ERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTRGP 188

Query: 216 GFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRNCLEIKELTLEKDSRELGKANRDL 275
             S         +  ++L +  + +G+ + +I      L+I+  TL      +G+  R L
Sbjct: 189 DHSL-----AAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYL 248

Query: 276 AEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNE 335
           AEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++ ALE   
Sbjct: 249 AEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLV 308

Query: 336 LSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKDVV----QHTDKDSETRAVV 395
           L+   +   G  S++   ++   N+ ++L R+DEA+ + +  +        +   T A V
Sbjct: 309 LASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASV 368

Query: 396 FISMGKVLCNQEKFTDAKRCLEIASGIFDKK-EATSPVEAAEAYSEISMQYETMNEFETA 455
           F+ + ++     K  ++K   E A  I++K    T+  E A   +EIS  YE+++E E A
Sbjct: 369 FVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEA 428

Query: 456 ISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLRESFGSKH 515
           + LL++S+ LLE  P +Q +   + AR+G +    G+ ++A    E A   LR + G K 
Sbjct: 429 LKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAA-GEKS 488

Query: 516 FGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMG 575
              G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+  Y AMG
Sbjct: 489 AFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMG 548

Query: 576 SYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKA 600
               AIE  EQV+   E    TA  +  + ++ L +L ++A
Sbjct: 549 RIEDAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEA 571

BLAST of Lag0002310 vs. ExPASy Swiss-Prot
Match: Q9LII8 (Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 7.5e-46
Identity = 144/497 (28.97%), Postives = 238/497 (47.89%), Query Frame = 0

Query: 118 EERDLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKP--SLLIAMTLQLMGS 177
           E  +LG+  LK   +L   GE+  KAL  A RA+   +K   G+    L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 178 VNYSLKKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAI 237
           +   L +++D++  L R+  +    +G     ED      A  ++L ++   MG+ E +I
Sbjct: 209 IYAGLGRYNDAVPVLERSIEIPMIEDG-----EDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 238 GNLRNCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQ--LGH 297
                 LEI+   L +    +G+  R LAEA+V  + F++A   C  AL+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 298 NSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALG 357
            S+E A DR+L+G+I     +++ ALE   L+   + +     D+   +    +  ++L 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 358 RYDEAI----NTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDK 417
           R+DEAI      L    Q   +   + A+V++ +  +     K  D+K   E A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 418 KEATSPV-EAAEAYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGW 477
               +P+ E A  + EIS  Y++MNE + A+ LL+R+L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 478 LLLLTGKVQEAIPYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMA 537
           +  + G   E+    + A    R S   K    G   N +G A ++      AA +F  A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 538 KDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELR-E 597
           K I++   GP+H D++    NL+  Y AMG    AIE  E VV   E    TA  E+  E
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 598 AQRMLQQLKRKAHGTSK 605
            QR+   LK    G SK
Sbjct: 629 KQRLAALLKEAGRGRSK 640

BLAST of Lag0002310 vs. ExPASy Swiss-Prot
Match: F4HSX9 (Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 5.6e-41
Identity = 143/518 (27.61%), Postives = 240/518 (46.33%), Query Frame = 0

Query: 114 ESAFEERDLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKD-DNGKPSLLIAMTLQL 173
           E + E  +L    L     L   G+   KAL    RA    +   +NGKP L   M L +
Sbjct: 138 EPSSENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHV 197

Query: 174 MGSVNYSLKKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRRE 233
             +V+  LK++++++  L R+  +    EG     E+      A  ++L +    +G+ E
Sbjct: 198 TAAVHCKLKEYNEAIPVLQRSVEIPVVEEG-----EEHALAKFAGLMQLGDTYAMVGQLE 257

Query: 234 EAIGNLRNCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLG 293
            +I      L I++  L ++   +G+  R LAEA V  L F +A   C  AL IH++   
Sbjct: 258 SSISCYTEGLNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGL 317

Query: 294 HNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIAL 353
             S+  A DRRL+G+I     +H+ ALE   L+   +   G  S++   +    +  ++L
Sbjct: 318 PGSIAEAADRRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSL 377

Query: 354 GRYDEAINTLKD--VVQHTDKDSETRAV--VFISMGKVLCNQEKFTDAKRCLEIASGIFD 413
            R+DEAI   +       T K     AV  V+I +  +     K  +AK   E A  I++
Sbjct: 378 SRFDEAICAYQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYE 437

Query: 414 KKE-ATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLG 473
                 SP E A   ++IS+  E+MNE E AI+LL+++L +    P ++     + A++G
Sbjct: 438 SHNLEISPEEIASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMG 497

Query: 474 WLLLLTGKVQEAIPYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAM 533
            L  + GK  E+    + A   LR +   +    G   N +G A ++L+  + A ++F  
Sbjct: 498 VLYYMMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEE 557

Query: 534 AKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELRE 593
           AK I++   GP+H +++    NL+ AY A+G    AI+    VV   E    TA     +
Sbjct: 558 AKCILEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTED 617

Query: 594 AQRMLQQLKRKAHGTSKIELSTKALPLPHSPTSSKTLQ 626
            +R L QL ++A   +  +  +    +    TSS  L+
Sbjct: 618 EKRRLAQLLKEAGNVTGRKAKSLKTLIDSDLTSSSALR 650

BLAST of Lag0002310 vs. ExPASy Swiss-Prot
Match: A0JM23 (Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 6.2e-16
Identity = 80/353 (22.66%), Postives = 150/353 (42.49%), Query Frame = 0

Query: 228  MGRREEAIGNLRNCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIH 287
            +G   +A+  L+  LEI+E  L+ D   + ++   LA  YV    F +A     +ALEI 
Sbjct: 940  LGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYVQSKKFGNAEQLYKQALEIS 999

Query: 288  KKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAAN 347
            +   G   + VA +   L V+Y    + ++A +  + S K+ +          A    + 
Sbjct: 1000 ENAYGSEHMRVARELDALAVLYQKQNKFEQAEQLRKKSLKIRQK--------SARRKGSM 1059

Query: 348  MQIALGRYDEAINTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIF 407
               AL R       L+       KD+   A     +G +   Q     A+  L+ +  + 
Sbjct: 1060 YGFALLRR----RALQLEELTLGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMR 1119

Query: 408  DKKEATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSAR-L 467
            ++       + A++ + ++  Y    +++ A  L +R+L +  +     H   + + + L
Sbjct: 1120 ERVLGADHPDCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHL 1179

Query: 468  GWLLLLTGKVQEAIPYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA 527
              L    GK+ +A+P  E A E  ++SFG KH  V     NL   Y ++++   A+ ++ 
Sbjct: 1180 AVLYKRKGKLDKAVPLYELAVEIRQKSFGPKHPSVATALVNLAVLYCQMKKQAEASPLYE 1239

Query: 528  MAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGS 580
             A  I + SLG  H    E  +NL+      G +  A E  ++ ++  E+  S
Sbjct: 1240 RAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDFEKAAELYKRAMEIKETETS 1280

BLAST of Lag0002310 vs. ExPASy Swiss-Prot
Match: Q6AZT7 (Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.4e-15
Identity = 79/353 (22.38%), Postives = 149/353 (42.21%), Query Frame = 0

Query: 228  MGRREEAIGNLRNCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIH 287
            +G   +A+  L+  LEI+E  L+ D   + ++   LA  Y+    F +A     +ALEI 
Sbjct: 929  LGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYMQSKKFGNAEQLYKQALEIS 988

Query: 288  KKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAAN 347
            +   G   + VA +   L V+Y    + ++A +  + S K+ +          A    + 
Sbjct: 989  ENAYGSEHLRVARELDALAVLYQKQNKFEQAEQLRKKSLKIRQK--------SARRKGSM 1048

Query: 348  MQIALGRYDEAINTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIF 407
               AL R       L+       KD+   A     +G +   Q     A+  L+ +  + 
Sbjct: 1049 YGFALLRR----RALQLEELTLGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMR 1108

Query: 408  DKKEATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSAR-L 467
            ++       + A++ + ++  Y    +++ A  L +R+L +  +     H   + + + L
Sbjct: 1109 ERVLGADHPDCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHL 1168

Query: 468  GWLLLLTGKVQEAIPYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA 527
              L    GK+ +A+P  E A +  ++SFG KH  V     NL   Y ++++   A  ++ 
Sbjct: 1169 AVLYKRKGKLDKAVPLYELAVDIRQKSFGPKHPSVATALVNLAVLYCQMKKQDDALPLYE 1228

Query: 528  MAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGS 580
             A  I + SLG  H    E  +NL+      G Y  A E  ++ ++  E+  S
Sbjct: 1229 RAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDYEKAAELYKRAMEIKETETS 1269

BLAST of Lag0002310 vs. ExPASy TrEMBL
Match: A0A6J1GPX7 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456463 PE=4 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 575/634 (90.69%), Postives = 601/634 (94.79%), Query Frame = 0

Query: 1   MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFT 60
           MRRNASISLL H+NR+NLRSLSSLISP  IS+PSPS SSPVS PHL PCTKNHGLFFK +
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKLS 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQR+ KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK LVYA+RAL ALDKDDNGKPSL +AMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 240
           K+FSDSLGYLNRANR+LGQLE  GFSAE +RPVLHAVQLELANVKTAMGRREEA+ NLR 
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELER QSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAWE HGS AEDELREAQRML+QLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNH 635
           G SKIELS KALPLPHS TSSKTLQ DI SS  H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 634

BLAST of Lag0002310 vs. ExPASy TrEMBL
Match: A0A6J1JWL0 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC111488134 PE=4 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 572/634 (90.22%), Postives = 596/634 (94.01%), Query Frame = 0

Query: 1   MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFT 60
           MRRNASISLL H+NR NLRSLSSLISP  IS+PS S SSPVST HL PCTKNHGLFFKF 
Sbjct: 1   MRRNASISLLFHSNRFNLRSLSSLISPKFISDPSFSYSSPVSTTHLNPCTKNHGLFFKFK 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQR+ KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK LVYA+RAL ALDKDDNGK SL +AMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKLSLPVAMTLQLLGSVNYSL 180

Query: 181 KKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 240
           K+FSDSLGYLNRANR+LGQLE  GFSAE +RPVLHAVQLELANVKTAMGRREEA+ NLR 
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKA RDLAEAYVAILNFKDAL FCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKAKRDLAEAYVAILNFKDALLFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLKRSLALLEKLPQEQHS GSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSVGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAWE HGS AEDELREAQRML+QLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNH 635
           G SKIELS KALPLPHS TSSKTLQ DI SS  H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQEH 634

BLAST of Lag0002310 vs. ExPASy TrEMBL
Match: A0A6J1CRC1 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Momordica charantia OX=3673 GN=LOC111013468 PE=4 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 573/635 (90.24%), Postives = 603/635 (94.96%), Query Frame = 0

Query: 3   RNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFTRF 62
           R ASISLLSH+NRLNLRS+SSLIS +   +P PS SSP+S  HL PC+K+HGLFFK TRF
Sbjct: 2   RKASISLLSHSNRLNLRSISSLIS-DPPPSPPPS-SSPLSAAHLNPCSKSHGLFFKLTRF 61

Query: 63  QANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEERDL 122
           QANP RNLDTLVEKRPQISSRQR++KEKSELEEAFESA+TVDEM+ AFKDMESAFEERDL
Sbjct: 62  QANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDL 121

Query: 123 GLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDN-GKPSLLIAMTLQLMGSVNYSLK 182
           GLASLKIGLKLDQEGEDPEK L+YASRAL ALD DDN GKPSLL+AMTLQLMGSVNYSLK
Sbjct: 122 GLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLK 181

Query: 183 KFSDSLGYLNRANRVLGQLEGKGFS-AEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 242
           KFSDSLGYLNRANRVL QLE KGFS AED+RPVLHAVQLELANVKTAMGRREEA+GNLR 
Sbjct: 182 KFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRK 241

Query: 243 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 302
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNF+DALPFCMKALEIHK QLGHNSVEVAH
Sbjct: 242 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAH 301

Query: 303 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 362
           DRRLLGVIY+GL+EH+KALEQNELSQKVLKNWGLNSDLL AEIDAANMQIALGRYDEAI+
Sbjct: 302 DRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIS 361

Query: 363 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 422
           TLKDV Q TDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE
Sbjct: 362 TLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 421

Query: 423 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 482
           AYSEISMQYETMNEFETAISLLKRSLA+LEKLPQEQHSEGSVSAR+GWLLLLTGKVQEAI
Sbjct: 422 AYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAI 481

Query: 483 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 542
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 482 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 541

Query: 543 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 602
           ADSIE+CQNLSKAYGAMGSYALAI+FQEQVVDAWESHGS+AEDEL+EAQRML+QLKRKAH
Sbjct: 542 ADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAH 601

Query: 603 GTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNHL 636
           GTSK ELSTKALPLPHSPTSSK+LQ +I  S   L
Sbjct: 602 GTSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQL 634

BLAST of Lag0002310 vs. ExPASy TrEMBL
Match: A0A1S3BR15 (nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 2.1e-309
Identity = 562/634 (88.64%), Postives = 594/634 (93.69%), Query Frame = 0

Query: 1   MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFT 60
           MRRNASIS+L + NRLNLRSLSSLISPNSISN  P  SSPVSTP+LKPCTKN+GLFFKFT
Sbjct: 1   MRRNASISILPYFNRLNLRSLSSLISPNSISN-LPLNSSPVSTPNLKPCTKNNGLFFKFT 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQAN SRNLDTLVEKRP++SSRQR++KEKS+LEEAFESADTVDEMVKAFK+MESAFEE+
Sbjct: 61  RFQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEK 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSL 180
           DLGLASLKIGLKLDQEGEDPE+ L+YA RAL ALDKDDN  PSL +AMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 180

Query: 181 KKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 240
           K+FSDSLGYLNRANR+LGQLE KGFSAEDVRPVLHAVQLELANVKTAMGRREEA+ NLR 
Sbjct: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
            LEIKEL LEKDSRELG ANRDLAEAYVAILNFKDALPFCMK LEIHKK+LG+NSVEVAH
Sbjct: 241 SLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELS+KVLKNWGLNSDLLRAEI+AANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 420
           TLKDVVQHTDKDSE R VVF SMGKVLCNQEKFTD KRCLEIA GIFDK+E TSP  AAE
Sbjct: 361 TLKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISM YETMN+FETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAI
Sbjct: 421 AYSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAI 480

Query: 481 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 600
           ADSIEAC NLSKAYGAMGSYALAIEFQ+QVVDAWESHGSTAEDELREA++ LQQLKRKA 
Sbjct: 541 ADSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQ 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNH 635
           GTSK ELSTKALPLPHS TSSKTLQ D+ +   H
Sbjct: 601 GTSKFELSTKALPLPHSSTSSKTLQPDLPAHQKH 633

BLAST of Lag0002310 vs. ExPASy TrEMBL
Match: A0A5A7UWT8 (Nephrocystin-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00740 PE=4 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 2.8e-309
Identity = 562/634 (88.64%), Postives = 594/634 (93.69%), Query Frame = 0

Query: 1   MRRNASISLLSHTNRLNLRSLSSLISPNSISNPSPSCSSPVSTPHLKPCTKNHGLFFKFT 60
           MRRNASIS+L + NRLNLRSLSSLISPNSISN  P  SSPVSTP+LKPCTKN+GLFFKFT
Sbjct: 1   MRRNASISVLPYFNRLNLRSLSSLISPNSISN-LPLNSSPVSTPNLKPCTKNNGLFFKFT 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQAN SRNLDTLVEKRP++SSRQR++KEKS+LEEAFESADTVDEMVKAFK+MESAFEE+
Sbjct: 61  RFQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEK 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVNYSL 180
           DLGLASLKIGLKLDQEGEDPE+ L+YA RAL ALDKDDN  PSL +AMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 180

Query: 181 KKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRN 240
           K+FSDSLGYLNRANR+LGQLE KGFSAEDVRPVLHAVQLELANVKTAMGRREEA+ NLR 
Sbjct: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
            LEIKEL LEKDSRELG ANRDLAEAYVAILNFKDALPFCMK LEIHKK+LG+NSVEVAH
Sbjct: 241 SLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELS+KVLKNWGLNSDLLRAEI+AANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 420
           TLKDVVQHTDKDSE R VVF SMGKVLCNQEKFTD KRCLEIA GIFDK+E TSP  AAE
Sbjct: 361 TLKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISM YETMN+FETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAI
Sbjct: 421 AYSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAI 480

Query: 481 PYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKAH 600
           ADSIEAC NLSKAYGAMGSYALAIEFQ+QVVDAWESHGSTAEDELREA++ LQQLKRKA 
Sbjct: 541 ADSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQ 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQTDIHSSPNH 635
           GTSK ELSTKALPLPHS TSSKTLQ D+ +   H
Sbjct: 601 GTSKFELSTKALPLPHSSTSSKTLQPDLPAHQKH 633

BLAST of Lag0002310 vs. TAIR 10
Match: AT2G31240.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 757.7 bits (1955), Expect = 7.6e-219
Identity = 405/626 (64.70%), Postives = 503/626 (80.35%), Query Frame = 0

Query: 3   RNASISLLS--HTNRLNLRSLSSLISPNSISNPSPSCSSP---VSTPHLKPCTKNHGLFF 62
           ++ SISL+   H +++    LS  ++  S S+ S S  SP   +ST     C + + L  
Sbjct: 2   KSLSISLIRRLHFHKIRPIPLSIHLNAASSSSSSSSLISPAREIST----SCRRTNDLIK 61

Query: 63  KFTRFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEEAFESADTVDEMVKAFKDMESAF 122
           +  +F  NP RNL+T VE+ P+ISS     KEK +LEEAFESA+T DEMV+ FK+ME +F
Sbjct: 62  R--QFWVNPYRNLNTHVEEPPEISSSD---KEKIDLEEAFESANTTDEMVRLFKEMELSF 121

Query: 123 EERDLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKPSLLIAMTLQLMGSVN 182
           E  +LGL++LK+GL LD+EGEDPEK L YA +AL + D D N KP+LL+AM  QLMGS N
Sbjct: 122 EGNELGLSALKLGLHLDREGEDPEKVLSYADKALKSFDGDGN-KPNLLVAMASQLMGSAN 181

Query: 183 YSLKKFSDSLGYLNRANRVLGQLEGKG-FSAEDVRPVLHAVQLELANVKTAMGRREEAIG 242
           Y LK+FSDSLGYLNRANR+L +LE  G    EDVRPVLHAVQLELANVK AMGRREEAI 
Sbjct: 182 YGLKRFSDSLGYLNRANRILVKLEADGDCVVEDVRPVLHAVQLELANVKNAMGRREEAIE 241

Query: 243 NLRNCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSV 302
           NL+  LEIKE+T ++DS+E+G ANR LA+AYVA+LNF +ALP+ +KALEIHKK+LG+NS 
Sbjct: 242 NLKKSLEIKEMTFDEDSKEMGVANRSLADAYVAVLNFNEALPYALKALEIHKKELGNNSA 301

Query: 303 EVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYD 362
           EVA DRRLLGVIYSGLE+H KALEQN LSQ+VLKNWG+  +L+RAEIDAANM++ALG+Y+
Sbjct: 302 EVAQDRRLLGVIYSGLEQHDKALEQNRLSQRVLKNWGMKLELIRAEIDAANMKVALGKYE 361

Query: 363 EAINTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPV 422
           EAI+ LK VVQ TDKDSE RA+VFISM K L NQ+KF ++KRCLE A  I +KKE   PV
Sbjct: 362 EAIDILKSVVQQTDKDSEMRAMVFISMSKALVNQQKFAESKRCLEFACEILEKKETALPV 421

Query: 423 EAAEAYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKV 482
           E AEAYSE++MQYE+MNEFETAISLL+++L +LEKLPQEQHSEGSVSAR+GWLLL +G+V
Sbjct: 422 EVAEAYSEVAMQYESMNEFETAISLLQKTLGILEKLPQEQHSEGSVSARIGWLLLFSGRV 481

Query: 483 QEAIPYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSL 542
            +A+PYLE AAE L+ESFG+KHFGVGY+YNNLGAAYLEL RPQSAAQMFA+AKDIMDVSL
Sbjct: 482 SQAVPYLESAAERLKESFGAKHFGVGYVYNNLGAAYLELGRPQSAAQMFAVAKDIMDVSL 541

Query: 543 GPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLK 602
           GP+H DSI+ACQNLSKAY  MG+Y+LA+EFQ++V++AW++HG +A+DE++EA+R+L+ L+
Sbjct: 542 GPNHVDSIDACQNLSKAYAGMGNYSLAVEFQQRVINAWDNHGDSAKDEMKEAKRLLEDLR 601

Query: 603 RKAH-GTSKIELSTKALPLPHSPTSS 622
            KA  G S  +L  KALPLP    SS
Sbjct: 602 LKARGGVSTNKLLNKALPLPKPSHSS 617

BLAST of Lag0002310 vs. TAIR 10
Match: AT4G10840.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 1.3e-48
Identity = 163/581 (28.06%), Postives = 275/581 (47.33%), Query Frame = 0

Query: 36  SCSSPVSTPHLKPCTKNHGLFFKFTRFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEE 95
           S  +P  TP L+    +       T+  +NP R   T ++K P  SS   R K       
Sbjct: 9   SVKTPSDTPPLRVAVPD-------TQPLSNPPR---TPMKKTP--SSTPSRSKPSPNRST 68

Query: 96  AFESADTVDEMVKAFKDMES-AFEERDLGLASLKIGLKLDQEGEDPEKALVYASRALNAL 155
             + + TV     A  D++  + +  DLG   LK+       GE P KAL YA RA  + 
Sbjct: 69  GKKDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSF 128

Query: 156 DK-----------DDNGKPSLLIAMTLQLMGSVNYSLKKFSDSLGYLNRANRVLGQLEGK 215
           ++             +G P L +AM+L ++ ++  SL +F +++  L RA +V     G 
Sbjct: 129 ERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTRGP 188

Query: 216 GFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRNCLEIKELTLEKDSRELGKANRDL 275
             S         +  ++L +  + +G+ + +I      L+I+  TL      +G+  R L
Sbjct: 189 DHSL-----AAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYL 248

Query: 276 AEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNE 335
           AEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++ ALE   
Sbjct: 249 AEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLV 308

Query: 336 LSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKDVV----QHTDKDSETRAVV 395
           L+   +   G  S++   ++   N+ ++L R+DEA+ + +  +        +   T A V
Sbjct: 309 LASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASV 368

Query: 396 FISMGKVLCNQEKFTDAKRCLEIASGIFDKK-EATSPVEAAEAYSEISMQYETMNEFETA 455
           F+ + ++     K  ++K   E A  I++K    T+  E A   +EIS  YE+++E E A
Sbjct: 369 FVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEA 428

Query: 456 ISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLRESFGSKH 515
           + LL++S+ LLE  P +Q +   + AR+G +    G+ ++A    E A   LR + G K 
Sbjct: 429 LKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAA-GEKS 488

Query: 516 FGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMG 575
              G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+  Y AMG
Sbjct: 489 AFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMG 548

Query: 576 SYALAIEFQEQVVDAWESHGSTAEDELREAQRMLQQLKRKA 600
               AIE  EQV+   E    TA  +  + ++ L +L ++A
Sbjct: 549 RIEDAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEA 571

BLAST of Lag0002310 vs. TAIR 10
Match: AT3G27960.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 186.8 bits (473), Expect = 5.4e-47
Identity = 144/497 (28.97%), Postives = 238/497 (47.89%), Query Frame = 0

Query: 118 EERDLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKDDNGKP--SLLIAMTLQLMGS 177
           E  +LG+  LK   +L   GE+  KAL  A RA+   +K   G+    L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 178 VNYSLKKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRREEAI 237
           +   L +++D++  L R+  +    +G     ED      A  ++L ++   MG+ E +I
Sbjct: 209 IYAGLGRYNDAVPVLERSIEIPMIEDG-----EDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 238 GNLRNCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQ--LGH 297
                 LEI+   L +    +G+  R LAEA+V  + F++A   C  AL+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 298 NSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALG 357
            S+E A DR+L+G+I     +++ ALE   L+   + +     D+   +    +  ++L 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 358 RYDEAI----NTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDK 417
           R+DEAI      L    Q   +   + A+V++ +  +     K  D+K   E A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 418 KEATSPV-EAAEAYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGW 477
               +P+ E A  + EIS  Y++MNE + A+ LL+R+L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 478 LLLLTGKVQEAIPYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMA 537
           +  + G   E+    + A    R S   K    G   N +G A ++      AA +F  A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 538 KDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELR-E 597
           K I++   GP+H D++    NL+  Y AMG    AIE  E VV   E    TA  E+  E
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 598 AQRMLQQLKRKAHGTSK 605
            QR+   LK    G SK
Sbjct: 629 KQRLAALLKEAGRGRSK 640

BLAST of Lag0002310 vs. TAIR 10
Match: AT4G10840.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 8.6e-45
Identity = 151/540 (27.96%), Postives = 255/540 (47.22%), Query Frame = 0

Query: 36  SCSSPVSTPHLKPCTKNHGLFFKFTRFQANPSRNLDTLVEKRPQISSRQRRVKEKSELEE 95
           S  +P  TP L+    +       T+  +NP R   T ++K P  SS   R K       
Sbjct: 9   SVKTPSDTPPLRVAVPD-------TQPLSNPPR---TPMKKTP--SSTPSRSKPSPNRST 68

Query: 96  AFESADTVDEMVKAFKDMES-AFEERDLGLASLKIGLKLDQEGEDPEKALVYASRALNAL 155
             + + TV     A  D++  + +  DLG   LK+       GE P KAL YA RA  + 
Sbjct: 69  GKKDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSF 128

Query: 156 DK-----------DDNGKPSLLIAMTLQLMGSVNYSLKKFSDSLGYLNRANRVLGQLEGK 215
           ++             +G P L +AM+L ++ ++  SL +F +++  L RA +V     G 
Sbjct: 129 ERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTRGP 188

Query: 216 GFSAEDVRPVLHAVQLELANVKTAMGRREEAIGNLRNCLEIKELTLEKDSRELGKANRDL 275
             S         +  ++L +  + +G+ + +I      L+I+  TL      +G+  R L
Sbjct: 189 DHSL-----AAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYL 248

Query: 276 AEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNE 335
           AEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++ ALE   
Sbjct: 249 AEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLV 308

Query: 336 LSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKDVV----QHTDKDSETRAVV 395
           L+   +   G  S++   ++   N+ ++L R+DEA+ + +  +        +   T A V
Sbjct: 309 LASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASV 368

Query: 396 FISMGKVLCNQEKFTDAKRCLEIASGIFDKK-EATSPVEAAEAYSEISMQYETMNEFETA 455
           F+ + ++     K  ++K   E A  I++K    T+  E A   +EIS  YE+++E E A
Sbjct: 369 FVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEA 428

Query: 456 ISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLRESFGSKH 515
           + LL++S+ LLE  P +Q +   + AR+G +    G+ ++A    E A   LR + G K 
Sbjct: 429 LKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAA-GEKS 488

Query: 516 FGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMG 559
              G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+  Y AMG
Sbjct: 489 AFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMG 530

BLAST of Lag0002310 vs. TAIR 10
Match: AT1G27500.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 4.0e-42
Identity = 143/518 (27.61%), Postives = 240/518 (46.33%), Query Frame = 0

Query: 114 ESAFEERDLGLASLKIGLKLDQEGEDPEKALVYASRALNALDKD-DNGKPSLLIAMTLQL 173
           E + E  +L    L     L   G+   KAL    RA    +   +NGKP L   M L +
Sbjct: 138 EPSSENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHV 197

Query: 174 MGSVNYSLKKFSDSLGYLNRANRVLGQLEGKGFSAEDVRPVLHAVQLELANVKTAMGRRE 233
             +V+  LK++++++  L R+  +    EG     E+      A  ++L +    +G+ E
Sbjct: 198 TAAVHCKLKEYNEAIPVLQRSVEIPVVEEG-----EEHALAKFAGLMQLGDTYAMVGQLE 257

Query: 234 EAIGNLRNCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLG 293
            +I      L I++  L ++   +G+  R LAEA V  L F +A   C  AL IH++   
Sbjct: 258 SSISCYTEGLNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGL 317

Query: 294 HNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIAL 353
             S+  A DRRL+G+I     +H+ ALE   L+   +   G  S++   +    +  ++L
Sbjct: 318 PGSIAEAADRRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSL 377

Query: 354 GRYDEAINTLKD--VVQHTDKDSETRAV--VFISMGKVLCNQEKFTDAKRCLEIASGIFD 413
            R+DEAI   +       T K     AV  V+I +  +     K  +AK   E A  I++
Sbjct: 378 SRFDEAICAYQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYE 437

Query: 414 KKE-ATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLG 473
                 SP E A   ++IS+  E+MNE E AI+LL+++L +    P ++     + A++G
Sbjct: 438 SHNLEISPEEIASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMG 497

Query: 474 WLLLLTGKVQEAIPYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAM 533
            L  + GK  E+    + A   LR +   +    G   N +G A ++L+  + A ++F  
Sbjct: 498 VLYYMMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEE 557

Query: 534 AKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELRE 593
           AK I++   GP+H +++    NL+ AY A+G    AI+    VV   E    TA     +
Sbjct: 558 AKCILEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTED 617

Query: 594 AQRMLQQLKRKAHGTSKIELSTKALPLPHSPTSSKTLQ 626
            +R L QL ++A   +  +  +    +    TSS  L+
Sbjct: 618 EKRRLAQLLKEAGNVTGRKAKSLKTLIDSDLTSSSALR 650

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899312.10.0e+0093.63protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida][more]
XP_023549125.10.0e+0090.85protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo][more]
XP_022954096.10.0e+0090.69protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita moschata][more]
KAG7014584.10.0e+0090.22Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. ar... [more]
KAG6576067.10.0e+0090.22Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
O816291.8e-4728.06Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 P... [more]
Q9LII87.5e-4628.97Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 P... [more]
F4HSX95.6e-4127.61Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 P... [more]
A0JM236.2e-1622.66Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2[more]
Q6AZT71.4e-1522.38Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GPX70.0e+0090.69protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
A0A6J1JWL00.0e+0090.22protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC11148813... [more]
A0A6J1CRC10.0e+0090.24protein KINESIN LIGHT CHAIN-RELATED 1 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A1S3BR152.1e-30988.64nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1[more]
A0A5A7UWT82.8e-30988.64Nephrocystin-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00740... [more]
Match NameE-valueIdentityDescription
AT2G31240.17.6e-21964.70Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.11.3e-4828.06Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G27960.15.4e-4728.97Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.28.6e-4527.96Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G27500.14.0e-4227.61Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 578..605
NoneNo IPR availableCOILSCoilCoilcoord: 432..452
NoneNo IPR availablePFAMPF13424TPR_12coord: 260..324
e-value: 4.9E-8
score: 33.2
coord: 466..531
e-value: 1.0E-9
score: 38.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 613..635
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 596..635
NoneNo IPR availablePANTHERPTHR47459:SF1KINESIN LIGHT CHAIN-RELATEDcoord: 3..623
NoneNo IPR availablePANTHERPTHR47459KINESIN LIGHT CHAIN-RELATEDcoord: 3..623
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 377..410
e-value: 35.0
score: 10.2
coord: 460..493
e-value: 200.0
score: 3.6
coord: 299..332
e-value: 450.0
score: 0.4
coord: 215..248
e-value: 41.0
score: 9.5
coord: 419..452
e-value: 9.5
score: 15.2
coord: 544..577
e-value: 30.0
score: 10.8
coord: 502..535
e-value: 31.0
score: 10.6
coord: 339..372
e-value: 88.0
score: 6.7
coord: 257..290
e-value: 18.0
score: 12.7
coord: 167..200
e-value: 250.0
score: 2.6
IPR019734Tetratricopeptide repeatPFAMPF13181TPR_8coord: 545..572
e-value: 0.0058
score: 16.7
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 419..452
score: 8.5849
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 415..612
e-value: 6.2E-28
score: 99.6
coord: 128..322
e-value: 1.5E-16
score: 62.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 337..413
e-value: 3.2E-5
score: 25.9
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 169..320
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 464..577
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 273..492

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002310.1Lag0002310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity