Lag0002290 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002290
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr4: 41335233 .. 41335658 (-)
RNA-Seq Expression	Lag0002290
Synteny	Lag0002290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGAAGAACAGATGGAGAGATCGAGTGAGGAAGTTCGAGTTCCAGTGATCGACATTCATTCAGAGAGGCTGAGCGAATGGAGTGTTGAAGCTCTGAGAGAAGCATGCAAGGAATGGGGATTCTTCTACATCAGAAACCATGGCGTTTCGAAGGAGTTTTACCGGAAACTGGTCGCCGGCGCCGACGAACTCTTTGGTTTTCCCGAAGAGACGAAGCGCAAAATGAAACTGGGAATCGAATCTTACACGCCGAGATTTGTGGTGTCGCCCTTCGTGGAGAGCTTCAAGGTCATGGGGCCTCACTTCTCTGCCTCTGCTGCTGCGCAAGGTTTCACTGAGTCGTTATTCGGCCACCACGCTGCTGAATTTAGGTCTACTTTTCTTTCCCTTCTCTTTATTTTACGGTGTTGCATTAGTCGATGA

mRNA sequence

ATGTCGGAAGAACAGATGGAGAGATCGAGTGAGGAAGTTCGAGTTCCAGTGATCGACATTCATTCAGAGAGGCTGAGCGAATGGAGTGTTGAAGCTCTGAGAGAAGCATGCAAGGAATGGGGATTCTTCTACATCAGAAACCATGGCGTTTCGAAGGAGTTTTACCGGAAACTGGTCGCCGGCGCCGACGAACTCTTTGGTTTTCCCGAAGAGACGAAGCGCAAAATGAAACTGGGAATCGAATCTTACACGCCGAGATTTGTGGTGTCGCCCTTCGTGGAGAGCTTCAAGGTCATGGGGCCTCACTTCTCTGCCTCTGCTGCTGCGCAAGGTTTCACTGAGTCGTTATTCGGCCACCACGCTGCTGAATTTAGGTCTACTTTTCTTTCCCTTCTCTTTATTTTACGGTGTTGCATTAGTCGATGA

Coding sequence (CDS)

ATGTCGGAAGAACAGATGGAGAGATCGAGTGAGGAAGTTCGAGTTCCAGTGATCGACATTCATTCAGAGAGGCTGAGCGAATGGAGTGTTGAAGCTCTGAGAGAAGCATGCAAGGAATGGGGATTCTTCTACATCAGAAACCATGGCGTTTCGAAGGAGTTTTACCGGAAACTGGTCGCCGGCGCCGACGAACTCTTTGGTTTTCCCGAAGAGACGAAGCGCAAAATGAAACTGGGAATCGAATCTTACACGCCGAGATTTGTGGTGTCGCCCTTCGTGGAGAGCTTCAAGGTCATGGGGCCTCACTTCTCTGCCTCTGCTGCTGCGCAAGGTTTCACTGAGTCGTTATTCGGCCACCACGCTGCTGAATTTAGGTCTACTTTTCTTTCCCTTCTCTTTATTTTACGGTGTTGCATTAGTCGATGA

Protein sequence

MSEEQMERSSEEVRVPVIDIHSERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGADELFGFPEETKRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFRSTFLSLLFILRCCISR

Homology

[Close]

BLAST of Lag0002290 vs. NCBI nr
Match: KAG6576056.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 163.3 bits (412), Expect = 1.5e-36
Identity = 75/110 (68.18%), Postives = 91/110 (82.73%), Query Frame = 0


Query: 15  VPVIDIHSERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGADELFGFPEETKR 74
           VPVID+ S+ L+E +  +L  AC E GFF+IRNHGVSKEFY KL++GADELF  PEETK+
Sbjct: 6   VPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEF 125
           ++K+G+ SYTPRFVVSPF ESF+V GP+FS SAA  GFT+SLFGHHA EF
Sbjct: 66  QLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEF 115

[Close]

BLAST of Lag0002290 vs. NCBI nr
Match: XP_022953358.1 (gibberellin 20-oxidase-like protein [Cucurbita moschata])

HSP 1 Score: 163.3 bits (412), Expect = 1.5e-36
Identity = 75/110 (68.18%), Postives = 91/110 (82.73%), Query Frame = 0


Query: 15  VPVIDIHSERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGADELFGFPEETKR 74
           VPVID+  + L+E +  +L   C E GFF+IRNHGVSKEFY KL++GADELF  PEETK+
Sbjct: 6   VPVIDLSCQSLTESTASSLLRTCNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEF 125
           ++K+G+ SYTPRFVVSPF ESF+V GP+FSAS AAQGFT+SLFGHHA EF
Sbjct: 66  QLKMGVSSYTPRFVVSPFFESFRVSGPNFSASTAAQGFTQSLFGHHATEF 115

[Close]

BLAST of Lag0002290 vs. NCBI nr
Match: XP_022991455.1 (gibberellin 20-oxidase-like protein [Cucurbita maxima])

HSP 1 Score: 162.5 bits (410), Expect = 2.6e-36
Identity = 75/110 (68.18%), Postives = 93/110 (84.55%), Query Frame = 0


Query: 15  VPVIDIHSERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGADELFGFPEETKR 74
           VPVID+  + L+E +  +L  AC + GFF+IRNHGVSKEFY KL++GADELF  PEETK+
Sbjct: 6   VPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEF 125
           ++K+G+ SYTPRF+VSPF ESF+V GP+FSASAAAQGFT+SLFGHHA EF
Sbjct: 66  QLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEF 115

[Close]

BLAST of Lag0002290 vs. NCBI nr
Match: XP_038899419.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 161.0 bits (406), Expect = 7.5e-36
Identity = 74/113 (65.49%), Postives = 93/113 (82.30%), Query Frame = 0


Query: 15  VPVIDIHSERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGAD-ELFGFPEETK 74
           +PV+DI+S   S   + +L +ACKEWGFF+IRNHG+ KEFY KL++ AD  LF FP+ETK
Sbjct: 10  IPVMDIYSHSFSNSDISSLSKACKEWGFFFIRNHGIPKEFYGKLISDADRRLFSFPDETK 69

Query: 75  RKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFRS 127
           RKMK+G+ SYTPRFVVSP +ESFKV+GP+FSASAAA GFT+S+FG  A EFR+
Sbjct: 70  RKMKVGVSSYTPRFVVSPSMESFKVLGPNFSASAAAHGFTDSIFGRQATEFRN 122

[Close]

BLAST of Lag0002290 vs. NCBI nr
Match: KAG6576052.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 159.5 bits (402), Expect = 2.2e-35
Identity = 74/110 (67.27%), Postives = 91/110 (82.73%), Query Frame = 0


Query: 15  VPVIDIHSERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGADELFGFPEETKR 74
           +PVIDI  + L+E +  +L  AC E+GFF+IRNHG+SKE Y+KL++GAD+LF  PEETK+
Sbjct: 6   IPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGADQLFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEF 125
           K+ +G  SYTPRFVVSPF ESFKV GP+FSASAAAQGFT+SLFGH A EF
Sbjct: 66  KLTMGASSYTPRFVVSPFFESFKVSGPNFSASAAAQGFTQSLFGHQATEF 115

[Close]

BLAST of Lag0002290 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 9.3e-21
Identity = 50/95 (52.63%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 13  VRVPVIDIHSERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGADELFGFPEET 72
           + +PV DI S+ LSE S+ +L++ACKEWGFFY+ NHGVS++ Y+KL   +  +F   +E 
Sbjct: 3   LELPVFDI-SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDE- 62

Query: 73  KRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASA 108
             KMK+G  +YTPRF+ SPF ES +V GP F ASA
Sbjct: 63  -EKMKMGASNYTPRFIASPFFESLRVSGPDFYASA 94

[Close]

BLAST of Lag0002290 vs. ExPASy Swiss-Prot
Match: Q76NT9 (1-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium discoideum OX=44689 GN=aco PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.0e-07
Identity = 28/71 (39.44%), Postives = 40/71 (56.34%), Query Frame = 0


Query: 14 RVPVIDIHS----ERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGADELFGFP 73
          ++P+IDI S    E       + + +ACKE+GFFYI+NHGV +E    L     + F  P
Sbjct: 7  KLPIIDISSFQNNENDKNQVAKEINKACKEYGFFYIKNHGVDQELIENLQNVIKKFFSLP 66

Query: 74 EETKRKMKLGI 81
           E K K K+G+
Sbjct: 67 LEIKMKWKMGL 77

[Close]

BLAST of Lag0002290 vs. ExPASy Swiss-Prot
Match: A6BM06 (1-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium mucoroides OX=31287 GN=aco PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.0e-07
Identity = 28/71 (39.44%), Postives = 40/71 (56.34%), Query Frame = 0


Query: 14 RVPVIDIHS----ERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGADELFGFP 73
          ++P+IDI S    E       + + +ACKE+GFFYI+NHGV +E    L     + F  P
Sbjct: 7  KLPIIDISSFQNNENDKNQVAKEINKACKEYGFFYIKNHGVDQELIENLQNVIKKFFSLP 66

Query: 74 EETKRKMKLGI 81
           E K K K+G+
Sbjct: 67 LEIKMKWKMGL 77

[Close]

BLAST of Lag0002290 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 3.4e-07
Identity = 31/85 (36.47%), Postives = 44/85 (51.76%), Query Frame = 0


Query: 12  EVRVPVIDIH---SERLSEWSVEALREACKEWGFFYIRNHGVSKEFYRKLVAGADELFGF 71
           ++ +P+ID+    S    +  VE L  ACKEWGFF + NHG+   F  K+ +   + F  
Sbjct: 50  KIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNL 109

Query: 72  PEETKRKM---KLGIESYTPRFVVS 91
           P E K+K       IE +   FVVS
Sbjct: 110 PMEEKKKFWQRPDEIEGFGQAFVVS 134

[Close]

BLAST of Lag0002290 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 2.9e-06
Identity = 29/69 (42.03%), Postives = 38/69 (55.07%), Query Frame = 0


Query: 8   RSSEEVRVPVIDIHSERLSEWSV-EALREACKEWGFFYIRNHGVSKEFYRKLVAGADELF 67
           +   +  +PVIDI    L E SV +A+ +A +EWGFF + NHGVS E    +       F
Sbjct: 55  KEDSDESIPVIDI--SNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFF 114

Query: 68  GFPEETKRK 76
           G P E KRK
Sbjct: 115 GLPVEEKRK 121

The following BLAST results are available for this feature:

BLAST of Lag0002290 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6576056.1	1.5e-36	68.18	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022953358.1	1.5e-36	68.18	gibberellin 20-oxidase-like protein [Cucurbita moschata]	[more]
XP_022991455.1	2.6e-36	68.18	gibberellin 20-oxidase-like protein [Cucurbita maxima]	[more]
XP_038899419.1	7.5e-36	65.49	gibberellin 20-oxidase-like protein [Benincasa hispida]	[more]
KAG6576052.1	2.2e-35	67.27	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro...	[more]

BLAST of Lag0002290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	9.3e-21	52.63	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q76NT9	2.0e-07	39.44	1-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium discoideum OX=44689 G...	[more]
A6BM06	2.0e-07	39.44	1-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium mucoroides OX=31287 G...	[more]
Q39224	3.4e-07	36.47	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
Q9C899	2.9e-06	42.03	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 15..77 e-value: 1.8E-16 score: 61.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..127 e-value: 1.3E-17 score: 66.4
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 9..127
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 9..127
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 9..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002290.1	Lag0002290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity