Lag0002240 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002240
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	LITAF domain-containing protein
Location	chr4: 40813995 .. 40815713 (+)
RNA-Seq Expression	Lag0002240
Synteny	Lag0002240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAAGAACGACGAGCCGGTCGTCGGAGTTCCATTCTATGTGGAGAACAACGCGTATCAGGCCGGACAGATCCCACCGAACGCCATTTTTGGCGATCCTCAGGGCGTTCCTATCCAGCAAACCATGTACCGCGACACTCCTGCTCCTTTCTACTGTGTTTATTGTGGGAATTCCGGTCTCACCGACATCAGGTGCCTTTTCTACCTTCTCTCTACCACTGCTTCTAGGTTATTTTTCTCCTGTCTTTGATCTGTTTGAAATTCTGTGTTTAATTATTGCGTTAATTGCCGTTCTGTTTCTCCATGATTATCCCCTCTCGGAAAATAATGGAAATTGCTAGCTTTAATCGAATTGGCAATGAAATTTTCGAATTCGTTTATTGAAACTTGTATGTGATTGATCAATGGGCCTTCCCCATATTTGTCTTTGACCTAATTTTCTTCTTTAGGATTAGAGGTACTTGTACGCGATTAAATTTCGTGAGCCACGGAACTTCATCTTGCTTACAACATAAGGCAGGAATTGTGAAATGATTTGAGTGACTTTTACCTATATAATTACATTGCTTGGATGTTTAGACACCTGATTCTGTTTTATAAAGTATACTGTTTTTCGCTTATATTTATTTCAAAATCCATTCAATTATGTGTACCAGATTGTGGCAATCTTGAGCGATTGCTGCATTGTTTGGTTTCTTCTCCCTATGGATAAATCTAATTGTTTTCACGTCAGTTTCTTTGGTTTTGAATTTGTTTAAATACTCAGTTAGGACGCAGGTGCAAGGTGATAGAAATTCAAGGGTTCCCTTAGCGCTGATAACTAATAGCTACACCATACAAAGTCTTGTTTGGCTATAAATACTTTTTAATGTTAAAAACATTCAAATGACTTTTACATTAGATTCTCTCTATCTTAAAAAATAGCATTATAGGGAGTGTGGGAGCATTAATTTGCATTGCAACAGGGGCACTTGAAATTTTATCTTGCTGGGTTTTTCAATCAAATCTATTTTTTTGGTATTGCATTGAAATTTATGTCTTGTTTATTCTTTGTTCAACATCAACAATGACAATTACAATAATTGCATAAATTAAGTAGGTTACCATTTTACCATCTTAGTTTTTAGTGGGAGCTGCATAAAGAAATATAAGTCCAAATGTTAGGTTTATATACACTCTTAAAAAATGTTGTTGCTCATAAGCTCAAGACTTCTTGTAGTAGGGGCAGCAATATAATTGATCATGGTTGGGAAGCCAGAGTTTCTGTATATATATTTTGTCTAAGAAGATATAGAAAGGGGGGATAGGTCATAGGTGGATTTATATGTTTATATTCGGTCAATAAATATGCTTCTTCTTCATCTGGTTATGCTACTTTTTTGACCTACAGATCAAAGATAAGTTCAGCTGCTTTCATTGGTTGTATGGTGCCTTTTATGTTGGGAGTATGCTTTCTTTGTCCTTCAATGGACTGCCTTTGGCATAAGTACCACTATTGCCCCAGCTGCAAGGAAAAGGTATGACTATTTTCTGGTCATTTTTCATTCTGTCAGTTGCTTCTTGTAATCTACATTTCTGATAGAGCCAAGTACATGATTGGAGTAATTAACTATACTATCCATGTTTTGATTAGGTTGGTGATTTTGAGAAATTGGATAGGTGTGCTGTGATGGATCCTCCACACTGGACCCAGCCAAGCTTTGCATTGCCTGCTTGA

mRNA sequence

ATGTCGAAGAACGACGAGCCGGTCGTCGGAGTTCCATTCTATGTGGAGAACAACGCGTATCAGGCCGGACAGATCCCACCGAACGCCATTTTTGGCGATCCTCAGGGCGTTCCTATCCAGCAAACCATGTACCGCGACACTCCTGCTCCTTTCTACTGTGTTTATTGTGGGAATTCCGGTCTCACCGACATCAGATCAAAGATAAGTTCAGCTGCTTTCATTGGTTGTATGGTGCCTTTTATGTTGGGAGTATGCTTTCTTTGTCCTTCAATGGACTGCCTTTGGCATAAGTACCACTATTGCCCCAGCTGCAAGGAAAAGGTTGGTGATTTTGAGAAATTGGATAGGTGTGCTGTGATGGATCCTCCACACTGGACCCAGCCAAGCTTTGCATTGCCTGCTTGA

Coding sequence (CDS)

ATGTCGAAGAACGACGAGCCGGTCGTCGGAGTTCCATTCTATGTGGAGAACAACGCGTATCAGGCCGGACAGATCCCACCGAACGCCATTTTTGGCGATCCTCAGGGCGTTCCTATCCAGCAAACCATGTACCGCGACACTCCTGCTCCTTTCTACTGTGTTTATTGTGGGAATTCCGGTCTCACCGACATCAGATCAAAGATAAGTTCAGCTGCTTTCATTGGTTGTATGGTGCCTTTTATGTTGGGAGTATGCTTTCTTTGTCCTTCAATGGACTGCCTTTGGCATAAGTACCACTATTGCCCCAGCTGCAAGGAAAAGGTTGGTGATTTTGAGAAATTGGATAGGTGTGCTGTGATGGATCCTCCACACTGGACCCAGCCAAGCTTTGCATTGCCTGCTTGA

Protein sequence

MSKNDEPVVGVPFYVENNAYQAGQIPPNAIFGDPQGVPIQQTMYRDTPAPFYCVYCGNSGLTDIRSKISSAAFIGCMVPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVMDPPHWTQPSFALPA

Homology

[Close]

BLAST of Lag0002240 vs. NCBI nr
Match: XP_004140553.1 (GSH-induced LITAF domain protein [Cucumis sativus] >KGN46422.1 hypothetical protein Csa_005550 [Cucumis sativus])

HSP 1 Score: 275.4 bits (703), Expect = 2.6e-70
Identity = 122/133 (91.73%), Postives = 125/133 (93.98%), Query Frame = 0


Query: 1   MSKNDEPVVGVPFYVENNAYQAGQIPPNAIFGDPQGVPIQQTMYRDTPAPFYCVYCGNSG 60
           MSKN EPVVGVP YV NN YQAGQIPPNAIFGDP GVPIQQTMYRDTPAPF CVYCGNSG
Sbjct: 1   MSKNHEPVVGVPLYVGNNPYQAGQIPPNAIFGDPHGVPIQQTMYRDTPAPFNCVYCGNSG 60

Query: 61  LTDIRSKISSAAFIGCMVPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM 120
           LT IRSKISSAA +GCM+PFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM
Sbjct: 61  LTTIRSKISSAAVVGCMMPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM 120

Query: 121 DPPHWTQPSFALP 134
           DPPHWTQPS+ALP
Sbjct: 121 DPPHWTQPSYALP 133

[Close]

BLAST of Lag0002240 vs. NCBI nr
Match: KAA0039847.1 (zf-LITAF-like domain-containing protein [Cucumis melo var. makuwa] >TYK24652.1 zf-LITAF-like domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 275.0 bits (702), Expect = 3.4e-70
Identity = 122/133 (91.73%), Postives = 125/133 (93.98%), Query Frame = 0


Query: 1   MSKNDEPVVGVPFYVENNAYQAGQIPPNAIFGDPQGVPIQQTMYRDTPAPFYCVYCGNSG 60
           MSKN EPVVGVP YV NN YQAGQIPPNAIFGDPQGVPIQQTMYRDTPAPF CVYCGNSG
Sbjct: 1   MSKNHEPVVGVPLYVGNNPYQAGQIPPNAIFGDPQGVPIQQTMYRDTPAPFNCVYCGNSG 60

Query: 61  LTDIRSKISSAAFIGCMVPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM 120
           LT IRSKIS AA +GCM+PFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM
Sbjct: 61  LTTIRSKISLAAVVGCMMPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM 120

Query: 121 DPPHWTQPSFALP 134
           DPPHWTQPS+ALP
Sbjct: 121 DPPHWTQPSYALP 133

[Close]

BLAST of Lag0002240 vs. NCBI nr
Match: XP_022954275.1 (GSH-induced LITAF domain protein [Cucurbita moschata] >XP_023547364.1 GSH-induced LITAF domain protein [Cucurbita pepo subsp. pepo] >KAG6576107.1 GSH-induced LITAF domain protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014627.1 GSH-induced LITAF domain protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273.5 bits (698), Expect = 9.9e-70
Identity = 121/134 (90.30%), Postives = 125/134 (93.28%), Query Frame = 0


Query: 1   MSKNDEPVVGVPFYVENNAYQAGQIPPNAIFGDPQGVPIQQTMYRDTPAPFYCVYCGNSG 60
           MSKNDEPVVG+PFYV NNAYQAGQIPPNAIFG PQGVPI QTMYRDTPAP+ CVYCGNSG
Sbjct: 1   MSKNDEPVVGIPFYVGNNAYQAGQIPPNAIFGHPQGVPIHQTMYRDTPAPYCCVYCGNSG 60

Query: 61  LTDIRSKISSAAFIGCMVPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM 120
           LT IRSKIS AA +GCM+PFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEK D CAVM
Sbjct: 61  LTSIRSKISLAAVVGCMMPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKSDVCAVM 120

Query: 121 DPPHWTQPSFALPA 135
           DPPHWTQPSFALPA
Sbjct: 121 DPPHWTQPSFALPA 134

[Close]

BLAST of Lag0002240 vs. NCBI nr
Match: XP_022991744.1 (GSH-induced LITAF domain protein [Cucurbita maxima])

HSP 1 Score: 273.1 bits (697), Expect = 1.3e-69
Identity = 120/134 (89.55%), Postives = 125/134 (93.28%), Query Frame = 0


Query: 1   MSKNDEPVVGVPFYVENNAYQAGQIPPNAIFGDPQGVPIQQTMYRDTPAPFYCVYCGNSG 60
           MSKNDEPVVG+PFYV NN+YQAGQIPPNAIFG PQGVPI QTMYRDTPAP+ CVYCGNSG
Sbjct: 1   MSKNDEPVVGIPFYVGNNSYQAGQIPPNAIFGHPQGVPIHQTMYRDTPAPYCCVYCGNSG 60

Query: 61  LTDIRSKISSAAFIGCMVPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM 120
           LT IRSKIS AA +GCM+PFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEK D CAVM
Sbjct: 61  LTSIRSKISLAAVVGCMMPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKSDACAVM 120

Query: 121 DPPHWTQPSFALPA 135
           DPPHWTQPSFALPA
Sbjct: 121 DPPHWTQPSFALPA 134

[Close]

BLAST of Lag0002240 vs. NCBI nr
Match: XP_038906445.1 (GSH-induced LITAF domain protein [Benincasa hispida])

HSP 1 Score: 273.1 bits (697), Expect = 1.3e-69
Identity = 120/134 (89.55%), Postives = 126/134 (94.03%), Query Frame = 0


Query: 1   MSKNDEPVVGVPFYVENNAYQAGQIPPNAIFGDPQGVPIQQTMYRDTPAPFYCVYCGNSG 60
           MSKNDEPVVG+P Y  NN YQAGQIP NAIFGDPQGVPIQQTMYRDTPAPFYCVYCGNSG
Sbjct: 1   MSKNDEPVVGIPLYDGNNPYQAGQIPSNAIFGDPQGVPIQQTMYRDTPAPFYCVYCGNSG 60

Query: 61  LTDIRSKISSAAFIGCMVPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM 120
           LTD+RSKISSAA +GCM PF+LGVCFL PSMDCLWHKYHYCPSCKEKVGDFEK+DRCAVM
Sbjct: 61  LTDVRSKISSAAVVGCMTPFLLGVCFLFPSMDCLWHKYHYCPSCKEKVGDFEKMDRCAVM 120

Query: 121 DPPHWTQPSFALPA 135
           DPP+WTQPSFALPA
Sbjct: 121 DPPNWTQPSFALPA 134

[Close]

BLAST of Lag0002240 vs. ExPASy Swiss-Prot
Match: Q94CD4 (GSH-induced LITAF domain protein OS=Arabidopsis thaliana OX=3702 GN=GILP PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.4e-55
Identity = 92/134 (68.66%), Postives = 106/134 (79.10%), Query Frame = 0


Query: 1   MSKNDEPVVGVPFYVENNAYQAGQIPPNAIFGDPQGVPIQQTMYRDTPAPFYCVYCGNSG 60
           M+K    V+GVP+Y   N YQAG +PPNAI+GDP G PIQQT+YRDTPAPF C+YCGN+G
Sbjct: 1   MAKEGTTVIGVPYYAGQNPYQAGIVPPNAIYGDPLGAPIQQTIYRDTPAPFNCLYCGNTG 60

Query: 61  LTDIRSKISSAAFIGCMVPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKLDRCAVM 120
           LT++RSK   AA + CM+PFMLG CFLCPSMDCLW+K H+CP C  KV DFEK D C VM
Sbjct: 61  LTNLRSKPGVAAVVACMMPFMLGFCFLCPSMDCLWNKQHHCPQCGNKVADFEKSDPCLVM 120

Query: 121 DPPHWTQPSFALPA 135
           DPP W QPSFALPA
Sbjct: 121 DPPQWKQPSFALPA 134

[Close]

BLAST of Lag0002240 vs. ExPASy Swiss-Prot
Match: Q9JLJ0 (Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog OS=Mus musculus OX=10090 GN=Litaf PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 2.6e-04
Identity = 28/102 (27.45%), Postives = 48/102 (47.06%), Query Frame = 0


Query: 19  AYQAGQIP-PNAIFGDPQGVPIQQTM-YRDTPAPFYCVYCGNSGLTDIRSKISSAAFIG- 78
           +Y    +P PNA     Q V +QQ + + D P    C  C    +T +     +  ++  
Sbjct: 60  SYYTQPVPVPNANAIAVQTVYVQQPVSFYDRPVQMCCPSCSKMIVTQLSYNAGALTWLSC 119

Query: 79  ---CMVPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKL 115
              C++  + G CF+   +D L    HYCP+CK  +G +++L
Sbjct: 120 GSLCLLGCVAGCCFIPFCVDALQDVDHYCPNCKALLGTYKRL 161

[Close]

BLAST of Lag0002240 vs. ExPASy Swiss-Prot
Match: P0C0T0 (Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog OS=Rattus norvegicus OX=10116 GN=Litaf PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 3.4e-04
Identity = 28/102 (27.45%), Postives = 48/102 (47.06%), Query Frame = 0


Query: 19  AYQAGQIP-PNAIFGDPQGVPIQQTM-YRDTPAPFYCVYCGNSGLTDIRSKISSAAFIG- 78
           +Y    +P PNA     Q V +QQ + + D P    C  C    +T +     +  ++  
Sbjct: 60  SYYTQPVPVPNANAIAVQTVYVQQPISFYDRPIQMCCPSCNKMIVTQLSYNAGALTWLSC 119

Query: 79  ---CMVPFMLGVCFLCPSMDCLWHKYHYCPSCKEKVGDFEKL 115
              C++  + G CF+   +D L    HYCP+CK  +G +++L
Sbjct: 120 GSLCLLGCVAGCCFIPFCVDALQDVDHYCPNCKALLGTYKRL 161

The following BLAST results are available for this feature:

BLAST of Lag0002240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140553.1	2.6e-70	91.73	GSH-induced LITAF domain protein [Cucumis sativus] >KGN46422.1 hypothetical prot...	[more]
KAA0039847.1	3.4e-70	91.73	zf-LITAF-like domain-containing protein [Cucumis melo var. makuwa] >TYK24652.1 z...	[more]
XP_022954275.1	9.9e-70	90.30	GSH-induced LITAF domain protein [Cucurbita moschata] >XP_023547364.1 GSH-induce...	[more]
XP_022991744.1	1.3e-69	89.55	GSH-induced LITAF domain protein [Cucurbita maxima]	[more]
XP_038906445.1	1.3e-69	89.55	GSH-induced LITAF domain protein [Benincasa hispida]	[more]

BLAST of Lag0002240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94CD4	2.4e-55	68.66	GSH-induced LITAF domain protein OS=Arabidopsis thaliana OX=3702 GN=GILP PE=1 SV...	[more]
Q9JLJ0	2.6e-04	27.45	Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog OS=Mus mus...	[more]
P0C0T0	3.4e-04	27.45	Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog OS=Rattus ...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006629	LPS-induced tumour necrosis factor alpha factor	SMART	SM00714	litaf	coord: 48..113 e-value: 9.8E-13 score: 58.3
IPR006629	LPS-induced tumour necrosis factor alpha factor	PFAM	PF10601	zf-LITAF-like	coord: 46..110 e-value: 2.3E-13 score: 50.2
IPR006629	LPS-induced tumour necrosis factor alpha factor	PROSITE	PS51837	LITAF	coord: 33..113 score: 11.666648
IPR037519	LITAF domain containing protein	PANTHER	PTHR23292	LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR	coord: 3..126
None	No IPR available	PANTHER	PTHR23292:SF37	BNAC09G43880D PROTEIN	coord: 3..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002240.1	Lag0002240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding