Lag0002219 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002219 vs. NCBI nr
Match: XP_038875622.1 (UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 468.0 bits (1203), Expect = 9.0e-128
Identity = 256/451 (56.76%), Postives = 333/451 (73.84%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVVG-------SEQPSIYKL 62
           E QPLLP   IR I  D+   K       VKNNL + L  + V        S QPSIYK+
Sbjct: 2   EEQPLLP---IRHIRNDNQVGKKDDVVIQVKNNLNKLLDSAAVDEMGPLGESTQPSIYKI 61

Query: 63  PECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGS 122
           PE +R++Q KAFEP+ VS+GPYHHG++HLV ME EK KAF  F   +GL +ESIV+ + +
Sbjct: 62  PEFMRKIQAKAFEPQLVSLGPYHHGKEHLVSMEEEKQKAFRRFVNDYGLNLESIVESMSN 121

Query: 123 MLKDLQGSYDELEDEWKEDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDML 182
            L++L G+YD+L+++WK+D A KFL++MIVDGCFML+ F +  CP SLS M+ ++ RDML
Sbjct: 122 NLENLWGAYDKLDEKWKQDGA-KFLEVMIVDGCFMLDFFKE--CPYSLSTMRWDIKRDML 181

Query: 183 LLENQLPMKLLEELY-SMTNNNQKQKNVKSLVSDFMCFKNKNKLDGKYLHILDMYRATLL 242
           LLENQLPM+LL+ELY +M N NQ+ ++++SL+   MC  N+  + G+ LHILDMYRA+LL
Sbjct: 182 LLENQLPMQLLQELYDNMPNTNQEDQSLRSLICQPMCI-NEEAVMGRELHILDMYRASLL 241

Query: 243 QPPVDREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDVDF-- 302
            PPVDR+DRS + K + Q     +S+P+ QIIP A QL +AGIKF+ S T++  DV F  
Sbjct: 242 YPPVDRKDRSGKTKVTYQ-----QSDPECQIIPQATQLHDAGIKFKSSPTKNLTDVSFNP 301

Query: 303 EGGVLKLPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSA 362
           + GVL+LP + VDDDTE  LLNVMAFEKL+V AG +VTSF+I M+NLID D DV LL S 
Sbjct: 302 KQGVLELPYIVVDDDTETTLLNVMAFEKLYVEAGSKVTSFVILMNNLIDVDRDVALLASD 361

Query: 363 GILVNALGDDQSAAGLFKLLAKGASMDSNSHISQVHQKVHRHCERKWNQWCASLKHNYFQ 422
            IL NALG+D+SAA LF LL KGA+MD +SHI+ VH KV++HC   WNQWCASLKH+YFQ
Sbjct: 362 KILANALGNDESAADLFSLLGKGAAMDLDSHITDVHYKVNKHCSLSWNQWCASLKHDYFQ 421

Query: 423 NPWAIISLIAALVGFLIILLQAVYQLLDYYR 444
           NPWAIISL AA+ GF I+++QA+YQ++DY+R
Sbjct: 422 NPWAIISLFAAVFGFAILIVQAIYQIVDYHR 440

BLAST of Lag0002219 vs. NCBI nr
Match: XP_022148888.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 433.3 bits (1113), Expect = 2.5e-117
Identity = 254/449 (56.57%), Postives = 323/449 (71.94%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVVGSEQPSIYKLPECIREV 62
           E  P +P+ II    +D+NE    +   +  N L    P   V +E  SIYK+P  +R+V
Sbjct: 2   ERPPSMPIRII----KDNNESAVVIRVRENLNQLLLDTP--AVEAEGLSIYKIPHFMRQV 61

Query: 63  QPKA-FEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGSMLKDLQ 122
           QPKA FEP+ VS GPYHHG+ HL RME EK KAF +F+T +GL VE IV+RV SML+D+Q
Sbjct: 62  QPKALFEPQLVSFGPYHHGESHLFRMELEKRKAFKNFQTRYGLPVEPIVERVWSMLEDVQ 121

Query: 123 GSYDELEDEWK-EDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDMLLLENQ 182
           G YDELE EWK ED A KFLQLM++DGCFMLE    +     LSNM+ ++ RDMLLLENQ
Sbjct: 122 GCYDELEGEWKLEDGAAKFLQLMVLDGCFMLEVLLAD-SGGWLSNMREDIVRDMLLLENQ 181

Query: 183 LPMKLLEELYSMTNNNQKQKNVKSLVSDFMCFKNK--NKLDGKYLHILDMYRATLLQPPV 242
           LPMKLLEEL+SM N++   KNVKSLV DFM   +K  +KL  +YLHIL+MY  TLL P V
Sbjct: 182 LPMKLLEELHSMANHSY-LKNVKSLVCDFMGIPDKVEDKLKVEYLHILEMYMDTLLLPKV 241

Query: 243 DREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDV--DFEGGV 302
             + R    +K + +E ++E + +IQII PA +L EAGI+FR S+++S  DV  D + GV
Sbjct: 242 VSQGR----QKGRSKEAAKELDSEIQIIWPATRLHEAGIRFRRSQSKSITDVWFDEKRGV 301

Query: 303 LKLPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSAGILV 362
           LKLP M VDDDTE   LNVMAFEKLH  AG +VT FI+ M+NLID D DV LL S  I++
Sbjct: 302 LKLPKMVVDDDTESTFLNVMAFEKLHKEAGSKVTCFILLMNNLIDVDQDVALLASNKIIL 361

Query: 363 NALGDDQSAAGLFKLLAKGASMD--SNSHISQVHQKVHRHCERKWNQWCASLKHNYFQNP 422
           NALG+DQ AA LF  LA+GA++D  S+SHI+ V + V  HC+++ ++WCASLKHNYFQ+P
Sbjct: 362 NALGNDQDAAELFGRLAQGAALDLKSDSHITHVQEMVIEHCKKRRHKWCASLKHNYFQHP 421

Query: 423 WAIISLIAALVGFLIILLQAVYQLLDYYR 444
           WAI+SLIAA +GF+I++LQAVYQ+ DYYR
Sbjct: 422 WAIVSLIAAALGFVILILQAVYQICDYYR 438

BLAST of Lag0002219 vs. NCBI nr
Match: XP_023513986.1 (UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 406.8 bits (1044), Expect = 2.5e-109
Identity = 232/451 (51.44%), Postives = 308/451 (68.29%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVV-------GSEQPSIYKL 62
           E  P L V    ++ RDDN    A     VK  L + L    +        SE  SIYK+
Sbjct: 2   EKHPSLQV----MLGRDDNIGDKADMVVQVKGTLDQLLDSPAILNMEAEQSSELLSIYKI 61

Query: 63  PECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGS 122
           P  + +  PKA+EPR VS+GPY+HG+QHL  ME EKLK F+SF+T   L+VESIV  V +
Sbjct: 62  PFFMTQTHPKAYEPRVVSLGPYNHGKQHLSPMELEKLKLFYSFKTRCLLDVESIVKGVST 121

Query: 123 MLKDLQGSYDELEDEWKEDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDML 182
           +L +L  SYD+LE+EW EDP GKFLQLMIVDGCFML    +  CP SL N+  ++ +DML
Sbjct: 122 ILDELMESYDKLEEEWTEDP-GKFLQLMIVDGCFMLGFLIN--CPDSLINVSPDIKQDML 181

Query: 183 LLENQLPMKLLEELYSMTNNNQK--QKNVKSLVSDFMCFKNKNKLDGKYLHILDMYRATL 242
           LLENQLPM LLE+LYS+   N +  Q++++ LVS+++    +N++    LHIL+MY+ +L
Sbjct: 182 LLENQLPMLLLEKLYSIAARNVQLPQQDLQKLVSNWLNIP-RNEVMKDCLHILEMYKESL 241

Query: 243 LQPPVDREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDV--D 302
           L PP+DR D S          + + S+P+ Q+IPPA +LREAGIKF+ S+T S  DV  D
Sbjct: 242 LHPPIDRTDWS---------VELDHSDPESQVIPPATKLREAGIKFKRSKTDSLTDVFFD 301

Query: 303 FEGGVLKLPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVS 362
            +GGVL LP + VDDDTE  LLNVMAFEKLH+ AG  VTSF+I M NLID + DV +L  
Sbjct: 302 AKGGVLWLPQLMVDDDTESTLLNVMAFEKLHMKAGRRVTSFVILMSNLIDDERDVAVLAG 361

Query: 363 AGILVNALGDDQSAAGLFKLLAKGASMDSNSHISQVHQKVHRHCERKWNQWCASLKHNYF 422
             +L NA+G+D+ AAGLF  L  GA+M  ++H++ VH+KV+ HC + WN+ CA+LKH+YF
Sbjct: 362 EKVLANAVGNDKEAAGLFNRLGSGAAMGLDTHMAGVHKKVNEHCNQPWNERCATLKHDYF 421

Query: 423 QNPWAIISLIAALVGFLIILLQAVYQLLDYY 443
           Q+PW IISL AA+ GF+I++LQA+YQ LDYY
Sbjct: 422 QSPWTIISLCAAIFGFIILILQAIYQFLDYY 435

BLAST of Lag0002219 vs. NCBI nr
Match: XP_023513987.1 (UPF0481 protein At3g47200-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 406.4 bits (1043), Expect = 3.2e-109
Identity = 232/450 (51.56%), Postives = 307/450 (68.22%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVV-------GSEQPSIYKL 62
           E  P L V    ++ RDDN    A     VK  L + L    +        SE  SIYK+
Sbjct: 2   EKHPSLQV----MLGRDDNIGDKADMVVQVKGTLDQLLDSPAILNMEAEQSSELLSIYKI 61

Query: 63  PECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGS 122
           P  + +  PKA+EPR VS+GPY+HG+QHL  ME EKLK F+SF+T   L+VESIV  V +
Sbjct: 62  PFFMTQTHPKAYEPRVVSLGPYNHGKQHLSPMELEKLKLFYSFKTRCLLDVESIVKGVST 121

Query: 123 MLKDLQGSYDELEDEWKEDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDML 182
           +L +L  SYD+LE+EW EDP GKFLQLMIVDGCFML    +  CP SL N+  ++ +DML
Sbjct: 122 ILDELMESYDKLEEEWTEDP-GKFLQLMIVDGCFMLGFLIN--CPDSLINVSPDIKQDML 181

Query: 183 LLENQLPMKLLEELYSMTNNN-QKQKNVKSLVSDFMCFKNKNKLDGKYLHILDMYRATLL 242
           LLENQLPM LLE+LYS+   N Q  ++++ LVS+++    +N++    LHIL+MY+ +LL
Sbjct: 182 LLENQLPMLLLEKLYSIAARNVQLPQDLQKLVSNWLNIP-RNEVMKDCLHILEMYKESLL 241

Query: 243 QPPVDREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDV--DF 302
            PP+DR D S          + + S+P+ Q+IPPA +LREAGIKF+ S+T S  DV  D 
Sbjct: 242 HPPIDRTDWS---------VELDHSDPESQVIPPATKLREAGIKFKRSKTDSLTDVFFDA 301

Query: 303 EGGVLKLPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSA 362
           +GGVL LP + VDDDTE  LLNVMAFEKLH+ AG  VTSF+I M NLID + DV +L   
Sbjct: 302 KGGVLWLPQLMVDDDTESTLLNVMAFEKLHMKAGRRVTSFVILMSNLIDDERDVAVLAGE 361

Query: 363 GILVNALGDDQSAAGLFKLLAKGASMDSNSHISQVHQKVHRHCERKWNQWCASLKHNYFQ 422
            +L NA+G+D+ AAGLF  L  GA+M  ++H++ VH+KV+ HC + WN+ CA+LKH+YFQ
Sbjct: 362 KVLANAVGNDKEAAGLFNRLGSGAAMGLDTHMAGVHKKVNEHCNQPWNERCATLKHDYFQ 421

Query: 423 NPWAIISLIAALVGFLIILLQAVYQLLDYY 443
           +PW IISL AA+ GF+I++LQA+YQ LDYY
Sbjct: 422 SPWTIISLCAAIFGFIILILQAIYQFLDYY 434

BLAST of Lag0002219 vs. NCBI nr
Match: XP_022960454.1 (UPF0481 protein At3g47200-like [Cucurbita moschata])

HSP 1 Score: 404.4 bits (1038), Expect = 1.2e-108
Identity = 231/451 (51.22%), Postives = 308/451 (68.29%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVV-------GSEQPSIYKL 62
           E  P L V    ++ RDDN    A     VK  L + L    +        SE  SIYK+
Sbjct: 2   EKHPSLQV----MLGRDDNIGDNADMVVRVKGTLDQLLDSPAILNMEAEQSSELLSIYKI 61

Query: 63  PECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGS 122
           P  + +  PKA+EP+ VS+GPY+HG+QHL  ME EKLK FHSF+    L+VESIV  V +
Sbjct: 62  PFFMTQTHPKAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKARCLLDVESIVRGVST 121

Query: 123 MLKDLQGSYDELEDEWKEDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDML 182
           +L +L  SYD+LE++WKEDP GKFLQLMIVDGCFML    +  CP SL N+  ++ +DML
Sbjct: 122 ILDELMESYDKLEEDWKEDP-GKFLQLMIVDGCFMLGFLIN--CPDSLINVSPDIKQDML 181

Query: 183 LLENQLPMKLLEELYSMTNNNQK--QKNVKSLVSDFMCFKNKNKLDGKYLHILDMYRATL 242
           LLENQLPM LLE+LYS+ + N +  Q+++K LVS+++    +N++    LHIL+MY+ +L
Sbjct: 182 LLENQLPMLLLEKLYSIADRNVQLPQQDLKKLVSNWLNIP-RNEVMKDCLHILEMYKESL 241

Query: 243 LQPPVDREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDV--D 302
           L PP+DR D S          +   S+P+ Q+IPPA +LREAGIKF+ S+T S  DV  D
Sbjct: 242 LHPPIDRTDWS---------VELGHSDPECQVIPPATKLREAGIKFKRSKTGSLTDVFFD 301

Query: 303 FEGGVLKLPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVS 362
            +GGVL LP + VDD+TE  LLNVMAFEKLH+ AG +VTSF+I M NLID + DV +L  
Sbjct: 302 AKGGVLWLPRLMVDDNTESTLLNVMAFEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAG 361

Query: 363 AGILVNALGDDQSAAGLFKLLAKGASMDSNSHISQVHQKVHRHCERKWNQWCASLKHNYF 422
             +L NA+G+D+ AAGLF  L  GA+M  ++H++ VH+KV+ HC + WN+ CA+LKH YF
Sbjct: 362 EQVLANAVGNDKEAAGLFNRLGSGAAMGLDTHMAWVHKKVNEHCNQPWNERCATLKHEYF 421

Query: 423 QNPWAIISLIAALVGFLIILLQAVYQLLDYY 443
           Q+PW IISL AA+ GF+I++LQA+YQ LDYY
Sbjct: 422 QSPWTIISLCAAIFGFIILILQAIYQFLDYY 435

BLAST of Lag0002219 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 6.7e-33
Identity = 120/431 (27.84%), Postives = 212/431 (49.19%), Query Frame = 0

Query: 46  GSEQPSIYKLPECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREK---LKAFHSFRTHH 105
           G E   I+++PE    + PKA++P+ VSIGPYH+G++HL  +++ K   L+ F       
Sbjct: 42  GKESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKK 101

Query: 106 GLEVESIVDRVGSMLKDLQGSYDELEDEWKEDPAGKFLQ-LMIVDGCFMLECF------- 165
            +E   +V  V  +   ++ SY E      E   G  L  +M++DGCF+L  F       
Sbjct: 102 DVEENVLVKAVVDLEDKIRKSYSE------ELKTGHDLMFMMVLDGCFILMVFLIMSGNI 161

Query: 166 ----DDNY-CPKSLSNMQAELARDMLLLENQLPMKLLEELYSMTNNNQKQKNVKSLVSDF 225
               D  +  P  LS++Q+    D+LLLENQ+P  +L+ LY + +      ++  +   F
Sbjct: 162 ELSEDPIFSIPWLLSSIQS----DLLLLENQVPFFVLQTLY-VGSKIGVSSDLNRIAFHF 221

Query: 226 MCFKNKNKLDGKY---------LHILDMYRATLLQPPVDREDRSRRGKKSQQEEQSEESN 285
             FKN    +G Y          H+LD+ R T L P     D++       Q  + +  N
Sbjct: 222 --FKNPIDKEGSYWEKHRNYKAKHLLDLIRETFL-PNTSESDKASSPHVQVQLHEGKSGN 281

Query: 286 ------PKIQIIPPARQLREAGIKFRESRTR--SFKDVDFEGGVLKLPVMEVDDDTEPGL 345
                   + +I  A++LR  GIKFR  R++  S  +V  +   L++P +  D       
Sbjct: 282 VPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISSFF 341

Query: 346 LNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSAGILV-NALGDDQSAAGLFKL 405
           LN +AFE+ +  +  E+T++I+ M  L++ + DV  L +  +++ N  G +   +  FK 
Sbjct: 342 LNCVAFEQFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKT 401

Query: 406 LAKGASMD-SNSHISQVHQKVHRHCERKWNQWCASLKHNYFQNPWAIISLIAALVGFLII 442
           ++K    +   S+++ V + V+ + ++ +N   A  +H +F++PW  +S  A L   L+ 
Sbjct: 402 ISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLFVILLT 458

BLAST of Lag0002219 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 3.8e-12
Identity = 46/170 (27.06%), Postives = 87/170 (51.18%), Query Frame = 0

Query: 266 IPPARQLREAGIKFRESRTRSFKDVDFE--GGVLKLPVMEVDDDTEPGLLNVMAFEKLHV 325
           IP    L +AG++F+ +   +   V F+   G   LPV+ +D +TE  L N++A+E  + 
Sbjct: 345 IPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNT 404

Query: 326 GAGGEVTSFIIHMDNLIDTDGDVELLVSAGILVNALGDDQSAAGLFKLLAKGASMDSNSH 385
                 T +   ++ +ID++ DV LL   G+LV+ L  DQ AA ++  ++K   +     
Sbjct: 405 SGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVRLTKVGF 464

Query: 386 ISQVHQKVHRHCERKWNQWCASLKHNYFQNPWAIISLIAALVGFLIILLQ 434
           + +  + V+R+   +W      L   Y    W I++ +AA++  +++ LQ
Sbjct: 465 LDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSLQ 514

BLAST of Lag0002219 vs. ExPASy TrEMBL
Match: A0A6J1D5C0 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111017450 PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 1.2e-117
Identity = 254/449 (56.57%), Postives = 323/449 (71.94%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVVGSEQPSIYKLPECIREV 62
           E  P +P+ II    +D+NE    +   +  N L    P   V +E  SIYK+P  +R+V
Sbjct: 2   ERPPSMPIRII----KDNNESAVVIRVRENLNQLLLDTP--AVEAEGLSIYKIPHFMRQV 61

Query: 63  QPKA-FEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGSMLKDLQ 122
           QPKA FEP+ VS GPYHHG+ HL RME EK KAF +F+T +GL VE IV+RV SML+D+Q
Sbjct: 62  QPKALFEPQLVSFGPYHHGESHLFRMELEKRKAFKNFQTRYGLPVEPIVERVWSMLEDVQ 121

Query: 123 GSYDELEDEWK-EDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDMLLLENQ 182
           G YDELE EWK ED A KFLQLM++DGCFMLE    +     LSNM+ ++ RDMLLLENQ
Sbjct: 122 GCYDELEGEWKLEDGAAKFLQLMVLDGCFMLEVLLAD-SGGWLSNMREDIVRDMLLLENQ 181

Query: 183 LPMKLLEELYSMTNNNQKQKNVKSLVSDFMCFKNK--NKLDGKYLHILDMYRATLLQPPV 242
           LPMKLLEEL+SM N++   KNVKSLV DFM   +K  +KL  +YLHIL+MY  TLL P V
Sbjct: 182 LPMKLLEELHSMANHSY-LKNVKSLVCDFMGIPDKVEDKLKVEYLHILEMYMDTLLLPKV 241

Query: 243 DREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDV--DFEGGV 302
             + R    +K + +E ++E + +IQII PA +L EAGI+FR S+++S  DV  D + GV
Sbjct: 242 VSQGR----QKGRSKEAAKELDSEIQIIWPATRLHEAGIRFRRSQSKSITDVWFDEKRGV 301

Query: 303 LKLPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSAGILV 362
           LKLP M VDDDTE   LNVMAFEKLH  AG +VT FI+ M+NLID D DV LL S  I++
Sbjct: 302 LKLPKMVVDDDTESTFLNVMAFEKLHKEAGSKVTCFILLMNNLIDVDQDVALLASNKIIL 361

Query: 363 NALGDDQSAAGLFKLLAKGASMD--SNSHISQVHQKVHRHCERKWNQWCASLKHNYFQNP 422
           NALG+DQ AA LF  LA+GA++D  S+SHI+ V + V  HC+++ ++WCASLKHNYFQ+P
Sbjct: 362 NALGNDQDAAELFGRLAQGAALDLKSDSHITHVQEMVIEHCKKRRHKWCASLKHNYFQHP 421

Query: 423 WAIISLIAALVGFLIILLQAVYQLLDYYR 444
           WAI+SLIAA +GF+I++LQAVYQ+ DYYR
Sbjct: 422 WAIVSLIAAALGFVILILQAVYQICDYYR 438

BLAST of Lag0002219 vs. ExPASy TrEMBL
Match: A0A6J1HB25 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461183 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 5.9e-109
Identity = 231/451 (51.22%), Postives = 308/451 (68.29%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVV-------GSEQPSIYKL 62
           E  P L V    ++ RDDN    A     VK  L + L    +        SE  SIYK+
Sbjct: 2   EKHPSLQV----MLGRDDNIGDNADMVVRVKGTLDQLLDSPAILNMEAEQSSELLSIYKI 61

Query: 63  PECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGS 122
           P  + +  PKA+EP+ VS+GPY+HG+QHL  ME EKLK FHSF+    L+VESIV  V +
Sbjct: 62  PFFMTQTHPKAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKARCLLDVESIVRGVST 121

Query: 123 MLKDLQGSYDELEDEWKEDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDML 182
           +L +L  SYD+LE++WKEDP GKFLQLMIVDGCFML    +  CP SL N+  ++ +DML
Sbjct: 122 ILDELMESYDKLEEDWKEDP-GKFLQLMIVDGCFMLGFLIN--CPDSLINVSPDIKQDML 181

Query: 183 LLENQLPMKLLEELYSMTNNNQK--QKNVKSLVSDFMCFKNKNKLDGKYLHILDMYRATL 242
           LLENQLPM LLE+LYS+ + N +  Q+++K LVS+++    +N++    LHIL+MY+ +L
Sbjct: 182 LLENQLPMLLLEKLYSIADRNVQLPQQDLKKLVSNWLNIP-RNEVMKDCLHILEMYKESL 241

Query: 243 LQPPVDREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDV--D 302
           L PP+DR D S          +   S+P+ Q+IPPA +LREAGIKF+ S+T S  DV  D
Sbjct: 242 LHPPIDRTDWS---------VELGHSDPECQVIPPATKLREAGIKFKRSKTGSLTDVFFD 301

Query: 303 FEGGVLKLPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVS 362
            +GGVL LP + VDD+TE  LLNVMAFEKLH+ AG +VTSF+I M NLID + DV +L  
Sbjct: 302 AKGGVLWLPRLMVDDNTESTLLNVMAFEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAG 361

Query: 363 AGILVNALGDDQSAAGLFKLLAKGASMDSNSHISQVHQKVHRHCERKWNQWCASLKHNYF 422
             +L NA+G+D+ AAGLF  L  GA+M  ++H++ VH+KV+ HC + WN+ CA+LKH YF
Sbjct: 362 EQVLANAVGNDKEAAGLFNRLGSGAAMGLDTHMAWVHKKVNEHCNQPWNERCATLKHEYF 421

Query: 423 QNPWAIISLIAALVGFLIILLQAVYQLLDYY 443
           Q+PW IISL AA+ GF+I++LQA+YQ LDYY
Sbjct: 422 QSPWTIISLCAAIFGFIILILQAIYQFLDYY 435

BLAST of Lag0002219 vs. ExPASy TrEMBL
Match: A0A6J1KYV8 (UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497633 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 3.9e-108
Identity = 230/451 (51.00%), Postives = 303/451 (67.18%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVV-------GSEQPSIYKL 62
           E QP L V    ++ RDDN    A     VK  L + L    +        SE  SIYK+
Sbjct: 2   EKQPPLQV----MVGRDDNIGDKADMVVRVKGTLDQLLDSPAILNMEAEQSSELLSIYKI 61

Query: 63  PECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGS 122
           P  + +  PKA+EP+ VS+GPY+HG+QHL  ME EKLK FHSF+T   L+VESIV  V +
Sbjct: 62  PFFMTQTHPKAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKTRCLLDVESIVRGVST 121

Query: 123 MLKDLQGSYDELEDEWKEDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDML 182
           +L +L  SYD LE+EW +DP GKFLQLMIVDGCFML       CP SL N+  ++ +DML
Sbjct: 122 ILDELMESYDRLEEEWTQDP-GKFLQLMIVDGCFMLGFLIS--CPNSLINVSPDIKQDML 181

Query: 183 LLENQLPMKLLEELYSMTNNNQK--QKNVKSLVSDFMCFKNKNKLDGKYLHILDMYRATL 242
           LLENQLPM LLE+LYS+   N +  Q++ K LV  ++    +N++    LHIL+MY+ +L
Sbjct: 182 LLENQLPMLLLEKLYSIAGRNVQLPQQDPKKLVCKWLSIP-QNEVMKDCLHILEMYKESL 241

Query: 243 LQPPVDREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDV--D 302
           L PP+DR D S          + + S+P+ Q+IPPA +L EAGIKF+ S+T S +DV  D
Sbjct: 242 LYPPIDRRDWS---------AELDHSDPECQVIPPATKLHEAGIKFKRSKTESLRDVWFD 301

Query: 303 FEGGVLKLPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVS 362
            + GVL LP + VDDDTE  +LNVMAFEKLH+ AG +VTSF+I M NLID + DV +L  
Sbjct: 302 TKRGVLWLPQLMVDDDTESTMLNVMAFEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAG 361

Query: 363 AGILVNALGDDQSAAGLFKLLAKGASMDSNSHISQVHQKVHRHCERKWNQWCASLKHNYF 422
             IL NA+G+D+ AAGLF  L  GA+M  +SH++ VH+ V+ HC + WN+ CA+LKH+YF
Sbjct: 362 EKILANAVGNDKEAAGLFSRLGSGAAMGLDSHMAGVHKMVNAHCNQPWNERCATLKHDYF 421

Query: 423 QNPWAIISLIAALVGFLIILLQAVYQLLDYY 443
           Q+PW IISL AA+ GF+I++LQA+YQ LDYY
Sbjct: 422 QSPWTIISLCAAIFGFIILILQAIYQFLDYY 435

BLAST of Lag0002219 vs. ExPASy TrEMBL
Match: A0A6J1KVQ6 (UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497633 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 5.0e-108
Identity = 230/450 (51.11%), Postives = 302/450 (67.11%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVV-------GSEQPSIYKL 62
           E QP L V    ++ RDDN    A     VK  L + L    +        SE  SIYK+
Sbjct: 2   EKQPPLQV----MVGRDDNIGDKADMVVRVKGTLDQLLDSPAILNMEAEQSSELLSIYKI 61

Query: 63  PECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGS 122
           P  + +  PKA+EP+ VS+GPY+HG+QHL  ME EKLK FHSF+T   L+VESIV  V +
Sbjct: 62  PFFMTQTHPKAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKTRCLLDVESIVRGVST 121

Query: 123 MLKDLQGSYDELEDEWKEDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDML 182
           +L +L  SYD LE+EW +DP GKFLQLMIVDGCFML       CP SL N+  ++ +DML
Sbjct: 122 ILDELMESYDRLEEEWTQDP-GKFLQLMIVDGCFMLGFLIS--CPNSLINVSPDIKQDML 181

Query: 183 LLENQLPMKLLEELYSMTNNN-QKQKNVKSLVSDFMCFKNKNKLDGKYLHILDMYRATLL 242
           LLENQLPM LLE+LYS+   N Q  ++ K LV  ++    +N++    LHIL+MY+ +LL
Sbjct: 182 LLENQLPMLLLEKLYSIAGRNVQLPQDPKKLVCKWLSIP-QNEVMKDCLHILEMYKESLL 241

Query: 243 QPPVDREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDV--DF 302
            PP+DR D S          + + S+P+ Q+IPPA +L EAGIKF+ S+T S +DV  D 
Sbjct: 242 YPPIDRRDWS---------AELDHSDPECQVIPPATKLHEAGIKFKRSKTESLRDVWFDT 301

Query: 303 EGGVLKLPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSA 362
           + GVL LP + VDDDTE  +LNVMAFEKLH+ AG +VTSF+I M NLID + DV +L   
Sbjct: 302 KRGVLWLPQLMVDDDTESTMLNVMAFEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAGE 361

Query: 363 GILVNALGDDQSAAGLFKLLAKGASMDSNSHISQVHQKVHRHCERKWNQWCASLKHNYFQ 422
            IL NA+G+D+ AAGLF  L  GA+M  +SH++ VH+ V+ HC + WN+ CA+LKH+YFQ
Sbjct: 362 KILANAVGNDKEAAGLFSRLGSGAAMGLDSHMAGVHKMVNAHCNQPWNERCATLKHDYFQ 421

Query: 423 NPWAIISLIAALVGFLIILLQAVYQLLDYY 443
           +PW IISL AA+ GF+I++LQA+YQ LDYY
Sbjct: 422 SPWTIISLCAAIFGFIILILQAIYQFLDYY 434

BLAST of Lag0002219 vs. ExPASy TrEMBL
Match: A0A6J1H6V9 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461034 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 2.4e-102
Identity = 227/458 (49.56%), Postives = 305/458 (66.59%), Query Frame = 0

Query: 3   ENQPLLPVPIIRIISRDDNEDKAAMSFDDVKNNLKEHLPCSVVGSEQPSIYKLPECIREV 62
           E QP L + +I    RDDN DK       V   L E  P     + + SIYKLP  +R+ 
Sbjct: 11  EKQPPLSIKLI----RDDNVDKPDDMVIQVMECLNE--PGLDSPTNKHSIYKLPFFMRQT 70

Query: 63  QP----KAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGSMLK 122
                 KAF+P+ VS GPYHHG++HL  MER+K KAF++ +T  GL VE+IV  V ++L 
Sbjct: 71  HSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKT-RGLPVEAIVREVSTILD 130

Query: 123 DLQGSYDELEDEWKEDPAGKFLQLMIVDGCFMLECFDDNYCPKSLSNMQAELARDMLLLE 182
           DL  SYD LE+EW +DP GKFL+LMIVDGCFML  F D  CP SL NM+ ++  + LLLE
Sbjct: 131 DLLESYDRLEEEWTQDP-GKFLKLMIVDGCFMLRLFCD--CPASLINMKMDIRLETLLLE 190

Query: 183 NQLPMKLLEELYSMT-----NNNQKQKNVKSLVSDFMCFKNKNKLDGKYLHILDMYRATL 242
           NQLP+KLL +L+S+      +    ++ ++ L+ +++    K+ LD ++LHILD+Y+A+L
Sbjct: 191 NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLD-EFLHILDLYKASL 250

Query: 243 LQPPVDREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDVDFE 302
           L PP+DR         ++  E+ E S  + Q+IPPA +L EAGIKF+ S+T+S KDV F+
Sbjct: 251 LCPPIDR---------TKWCEKGELSGAEFQVIPPATKLHEAGIKFKMSQTKSLKDVIFD 310

Query: 303 --GGVLKLPVMEVDDDTEPGLLNVMAFEKLHV-GAGGE----VTSFIIHMDNLIDTDGDV 362
              GVL LP++ VDD+TE   LNVMAFEKLHV GA  E    +TSF+I M NLID + DV
Sbjct: 311 EVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLIDDERDV 370

Query: 363 ELLVSAGILVNALGDDQSAAGLFKLLAKGASMDSNSHISQVHQKVHRHCERKWNQWCASL 422
            LL S G L NALG+D+ AA LF  L KG +M  N H+  VH+ ++ +C+  WN+ CA+L
Sbjct: 371 ALLSSKGTLANALGNDREAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWNERCATL 430

Query: 423 KHNYFQNPWAIISLIAALVGFLIILLQAVYQLLDYYRQ 445
           KH YFQNPW +ISL AA+ GFLI++LQA+YQLLDYY++
Sbjct: 431 KHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKK 448

BLAST of Lag0002219 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-54
Identity = 140/426 (32.86%), Postives = 224/426 (52.58%), Query Frame = 0

Query: 52  IYKLPECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVD 111
           IY++P+ ++E   K++ P+ VS+GP+HHG +HL+ M+R K +A +        ++E  +D
Sbjct: 141 IYRVPQYLQENNKKSYFPQTVSLGPFHHGNKHLLPMDRHKWRAVNMVMARTKHDIEMYID 200

Query: 112 RVGSMLKDLQGSYDELEDEWKEDPAGKFLQLMIVDGCFMLECF------------DDNYC 171
            +  +    +  Y+   D      + KF +++++DGCF+LE F            D N  
Sbjct: 201 AMKELEDRARACYEGPIDL----SSNKFSEMLVLDGCFVLELFRGADEGFSELGYDRNDP 260

Query: 172 PKSLSNMQAELARDMLLLENQLPMKLLEELYSMTNNNQKQKNVKS----------LVSDF 231
             ++      + RDM++LENQLP+ +L  L  +    + Q  + S          + +D 
Sbjct: 261 VFAMRGSMHSIQRDMVMLENQLPLFVLNRLLEIQLGKRHQTGLVSRLAVRFFDPLMPTDE 320

Query: 232 MCFKNKNKL------------DGKYLHILDMYRATLLQPPVDREDRSRRGKKSQQEEQSE 291
              K  + L            D   LH LD++R  LL+P  + E R  R + S +   ++
Sbjct: 321 PLTKTDDSLEQDKFFNPIADKDKGELHCLDVFRRNLLRPCSNPEPRLSRMRWSWRTRVAD 380

Query: 292 ESNPKIQIIPPARQLREAGIKFRESRTRSFKDVDFEGGVLKLPVMEVDDDTEPGLLNVMA 351
           +     Q+I    +LREAGIKFR  +T  F D+ F+ G L++P + + D T+    N++A
Sbjct: 381 KRQQ--QLIHCVTELREAGIKFRTRKTDRFWDIRFKNGYLEIPKLLIHDGTKSLFSNLIA 440

Query: 352 FEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSAGILVNALGDDQSAAGLFKLLAKGAS 411
           FE+ H+ +  ++TS+II MDNLID+  DV  L   GI+ + LG+D   A LF  L +  +
Sbjct: 441 FEQCHIDSSNDITSYIIFMDNLIDSSEDVRYLHYCGIIEHWLGNDYEVADLFNRLCQEVA 500

Query: 412 MD-SNSHISQVHQKVHRHCERKWNQWCASLKHNYFQNPWAIISLIAALVGFLIILLQAVY 443
            D  NS++SQ+  KV R+  RKWN   A LKH YF NPWA  S  AALV  ++ L Q+ +
Sbjct: 501 FDPQNSYLSQLSNKVDRNYSRKWNVLKAILKHKYFNNPWAYFSFFAALVLLVLTLFQSFF 560

BLAST of Lag0002219 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 203.4 bits (516), Expect = 3.9e-52
Identity = 146/487 (29.98%), Postives = 250/487 (51.33%), Query Frame = 0

Query: 4   NQPLLPVPIIRII--SRDDNEDKAAMSFDDVKNNLKEHLPCSVVGSEQPSIYKLPECIRE 63
           NQ     P I +I  S  D+ D   +S  D           ++ G  +  IY++P  ++E
Sbjct: 57  NQQTQSDPAIYVIKESPKDSRDDWVISITDKLEQAHRDDDTTLWG--KLCIYRVPYYLQE 116

Query: 64  VQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGSMLKDLQ 123
              K++ P+ VS+GPYHHG++ L  M+R K +A           V  ++ R    +K   
Sbjct: 117 NDNKSYFPQTVSLGPYHHGKKRLRSMDRHKWRA-----------VNRVLKRTNQGIKMYI 176

Query: 124 GSYDELEDEWK---EDP----AGKFLQLMIVDGCFMLECFDD------------NYCPKS 183
            +  ELE++ +   E P    + +F++++++DGCF+LE F              N    +
Sbjct: 177 DAMRELEEKARACYEGPLSLSSNEFIEMLVLDGCFVLELFRGAVEGFTELGYARNDPVFA 236

Query: 184 LSNMQAELARDMLLLENQLPMKLLEELYSMTNNNQKQKNVKSLVS-----------DFMC 243
           +      + RDM++LENQLP+ +L  L  +    + Q  + + ++           + + 
Sbjct: 237 MRGSMHSIQRDMVMLENQLPLFVLNRLLELQLGTRNQTGLVAQLAIRFFDPLMPTDEPLT 296

Query: 244 FKNKNKLDGKY--------------LHILDMYRATLLQPPVDREDRSRRGKKSQQEEQSE 303
              ++KL+                 LH LD++R +LL+     E R  R + S+    ++
Sbjct: 297 KSGQSKLENSLARDKSFDPFADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWSRNTRVAD 356

Query: 304 ESNPKIQIIPPARQLREAGIKFRESRTRSFKDVDFEGGVLKLPVMEVDDDTEPGLLNVMA 363
           +   + Q+I    +L+EAGIKFR  +T  F D+ F+ G L++P + + D T+   LN++A
Sbjct: 357 KR--RQQLIHCVTELKEAGIKFRRRKTDRFWDMQFKNGYLEIPRLLIHDGTKSLFLNLIA 416

Query: 364 FEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSAGILVNALGDDQSAAGLFKLLAKGAS 423
           FE+ H+ +  ++TS+II MDNLID+  DV  L   GI+ + LG D   A LF  L +   
Sbjct: 417 FEQCHIDSSNDITSYIIFMDNLIDSHEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVV 476

Query: 424 MDS-NSHISQVHQKVHRHCERKWNQWCASLKHNYFQNPWAIISLIAALVGFLIILLQAVY 444
            D+ +S++S++  +V+R+ + KWN W A+LKH YF NPWAI+S  AA++  ++   Q+ Y
Sbjct: 477 FDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVILLVLTFSQSFY 528

BLAST of Lag0002219 vs. TAIR 10
Match: AT3G50140.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 200.7 bits (509), Expect = 2.5e-51
Identity = 136/434 (31.34%), Postives = 225/434 (51.84%), Query Frame = 0

Query: 52  IYKLPECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSF--RTHHGLEVESI 111
           IY++P  +++    ++ P+ VS+GPYHHG +HL  M+  K +A +    RT  G+E    
Sbjct: 115 IYRVPLSLKKSDKNSYFPQAVSLGPYHHGDEHLRPMDYHKWRAVNMVMKRTKQGIE---- 174

Query: 112 VDRVGSMLKDLQGSYDELEDEWKEDPAG----KFLQLMIVDGCFMLECF----------- 171
                 M  D     +E      E P G    KF Q++++DGCF+L+ F           
Sbjct: 175 ------MYIDAMKELEERARACYEGPIGLSSNKFTQMLVLDGCFVLDLFRGAYEGFSKLG 234

Query: 172 -DDNYCPKSLSNMQAELARDMLLLENQLPMKLLEELYSMTNNNQKQKNV---------KS 231
            D N    ++      + RDML+LENQLP+ +L  L  +    Q Q  +           
Sbjct: 235 YDRNDPVFAMRGSMHSIRRDMLMLENQLPLFVLNRLLELQLGTQYQTGLVAQLAVRFFNP 294

Query: 232 LVSDFMCF-------KNKNKL-------DGKYLHILDMYRATLLQPPVDREDRSRRGKKS 291
           L+  +M         +N NK        + + LH LD++R +LLQP +  + R  R + S
Sbjct: 295 LMPTYMSSTKIENSQENNNKFFNPIADKEKEELHCLDVFRRSLLQPSLKPDPRLSRSRWS 354

Query: 292 QQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDVDFEGGVLKLPVMEVDDDTEP 351
           ++   +++     Q++    +LREAGIKF+  ++  F D+ F+ G L++P + + D T+ 
Sbjct: 355 RKPLVADKRQQ--QLLHCVTELREAGIKFKRRKSDRFWDIQFKNGCLEIPKLLIHDGTKS 414

Query: 352 GLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSAGILVNALGDDQSAAGLFK 411
              N++A+E+ H+ +  ++TS+II MDNLID+  D+  L    I+ + LG+D   A +F 
Sbjct: 415 LFSNLIAYEQCHIDSTNDITSYIIFMDNLIDSAEDIRYLHYYDIIEHWLGNDSEVADVFN 474

Query: 412 LLAKGASMD-SNSHISQVHQKVHRHCERKWNQWCASLKHNYFQNPWAIISLIAALVGFLI 444
            L +  + D  N+++S++  KV R+  RKWN   A+LKH YF NPWA  S  AA++  L+
Sbjct: 475 RLCQEVAFDLENTYLSELSNKVDRYYNRKWNVLKATLKHKYFSNPWAYFSFFAAVILLLL 534

BLAST of Lag0002219 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 198.7 bits (504), Expect = 9.5e-51
Identity = 134/435 (30.80%), Postives = 228/435 (52.41%), Query Frame = 0

Query: 52  IYKLPECIREVQPKAFEPRFVSIGPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVD 111
           IY++P  ++E   K++ P+ VS+GPYHHG++ L  MER K +A           +  ++ 
Sbjct: 115 IYRVPHYLQENDKKSYFPQTVSLGPYHHGKKRLRPMERHKWRA-----------LNKVLK 174

Query: 112 RVGSMLKDLQGSYDELEDEWK---EDP----AGKFLQLMIVDGCFMLECF--------DD 171
           R+   ++    +  ELE++ +   E P      +F +++++DGCF+LE F        + 
Sbjct: 175 RLKQRIEMYTNAMRELEEKARACYEGPISLSRNEFTEMLVLDGCFVLELFRGTVEGFTEI 234

Query: 172 NYCPK----SLSNMQAELARDMLLLENQLPMKLLEELYSMTNNNQKQKNVKSLVS----- 231
            Y       ++  +   + RDM++LENQLP+ +L+ L  +    Q Q  + + V+     
Sbjct: 235 GYARNDPVFAMRGLMHSIQRDMIMLENQLPLFVLDRLLELQLGTQNQTGIVAHVAVKFFD 294

Query: 232 ------DFMCFKNKNKL------------DGKYLHILDMYRATLLQPPVDREDRSRRGKK 291
                 + +   +++KL            D   LH LD++R +LLQ       RS   + 
Sbjct: 295 PLMPTGEALTKPDQSKLMNWLEKSLDTLGDKGELHCLDVFRRSLLQSSPTPNTRSLLKRL 354

Query: 292 SQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDVDFEGGVLKLPVMEVDDDTE 351
           ++     ++     Q++    +LREAG+KFR+ +T  F D++F+ G L++P + + D T+
Sbjct: 355 TRNTRVVDKRQQ--QLVHCVTELREAGVKFRKRKTDRFWDIEFKNGYLEIPKLLIHDGTK 414

Query: 352 PGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSAGILVNALGDDQSAAGLF 411
               N++AFE+ H+ +   +TS+II MDNLI++  DV  L   GI+ + LG D   A LF
Sbjct: 415 SLFSNLIAFEQCHIESSNHITSYIIFMDNLINSSEDVSYLHYCGIIEHWLGSDSEVADLF 474

Query: 412 KLLAKGASMD-SNSHISQVHQKVHRHCERKWNQWCASLKHNYFQNPWAIISLIAALVGFL 444
             L +    D  +SH+S++   V+R+  RKWN   A+L H YF NPWA  S  AA++  L
Sbjct: 475 NRLCQEVVFDPKDSHLSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLL 534

BLAST of Lag0002219 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 193.7 bits (491), Expect = 3.1e-49
Identity = 136/446 (30.49%), Postives = 225/446 (50.45%), Query Frame = 0

Query: 19  DDNEDKA----AMSFDDVKNNLKEHLPCSVVGSEQPSIYKLPECIREVQPKAFEPRFVSI 78
           D NE K      +S +D    L ++   +    +   IY++P  ++E   K++ P+ VSI
Sbjct: 72  DKNEQKLREIWVISLNDKMKTLGDN---ATTSWDNLCIYRVPPYLQENDTKSYMPQIVSI 131

Query: 79  GPYHHGQQHLVRMEREKLKAFHSFRTHHGLEVESIVDRVGSMLKDLQGSYDELEDEWKED 138
           GPYHHG +HL+ MER K +A +        ++E  +D +  + +  +  Y    +  + +
Sbjct: 132 GPYHHGHKHLMPMERHKWRAVNMVMARAKHDIEMYIDAMKELEEKARACYQGPINMNRNE 191

Query: 139 PAGKFLQLMIVDGCFMLECF--------DDNYCPK----SLSNMQAELARDMLLLENQLP 198
               F++++++DG F++E F        +  Y P      +  +   + RDM++LENQLP
Sbjct: 192 ----FIEMLVLDGVFIIEIFKGTSEGFQEIGYAPNDPVFGMRGLMQSIRRDMVMLENQLP 251

Query: 199 MKLLEELYSMTNNNQKQK-NVKSLVSDFMCFKNKNKL--DGKYLHILDMYRATLLQPP-V 258
             +L+ L  +   +   K NV+     F       ++  +   LH LD+ R  LLQ    
Sbjct: 252 WSVLKGLLQLQRPDVLDKVNVQLFQPFFQPLLPTREVLTEEGGLHCLDVLRRGLLQSSGT 311

Query: 259 DREDRSRRGKKSQQEEQSEESNPKIQIIPPARQLREAGIKFRESRTRSFKDVDFEGGVLK 318
             ED S   K+ Q            Q+I    +LR AG++F    T  F D++F+ G LK
Sbjct: 312 SDEDMSMVNKQPQ------------QLIHCVTELRNAGVEFMRKETGHFWDIEFKNGYLK 371

Query: 319 LPVMEVDDDTEPGLLNVMAFEKLHVGAGGEVTSFIIHMDNLIDTDGDVELLVSAGILVNA 378
           +P + + D T+   LN++AFE+ H+ +  ++TS+II MDNLI++  DV  L   GI+ N 
Sbjct: 372 IPKLLIHDGTKSLFLNLIAFEQCHIKSSKKITSYIIFMDNLINSSEDVSYLHHYGIIENW 431

Query: 379 LGDDQSAAGLFKLLAKGASMDSN-SHISQVHQKVHRHCERKWNQWCASLKHNYFQNPWAI 438
           LG D   + LF  L K    D N  ++S +  +V+ +  RKWN   A+L+H YF NPWA 
Sbjct: 432 LGSDSEVSDLFNGLGKEVIFDPNDGYLSALTGEVNIYYRRKWNYLKATLRHKYFNNPWAY 491

Query: 439 ISLIAALVGFLIILLQAVYQLLDYYR 444
            S IAA+   +    Q+ + +  Y++
Sbjct: 492 FSFIAAVTLLIFTFCQSFFAVFAYFK 498