Lag0002212 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002212
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	hsp70 nucleotide exchange factor FES1
Location	chr4: 40606272 .. 40610357 (-)
RNA-Seq Expression	Lag0002212
Synteny	Lag0002212

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGGTTGGAATCGAGAAGGAGAAGACTAAAGGCTGAAGGTTAGGGTTTTTGTTAAAGGGGTATATATATAGTCGGTTCGGTTCGATTTTTATTGGTTTTTTTCAATTAAGAACCGAACTGAAAAAATTGGTTTTCTCAAAAACTAAATTGAGATGTCCAGATTTTTGCTAAATAACTAAACCAAACCTATGGATTGGTTCGATTTGGTTGGGTTTGTCGGTTTTTCGGTTATCGCTTGCACTCCTAACATAAGCATAGCTCAACTGGCATGAGACTTGTACTAATGACCTAAAGGTTTGTGGTTCGAAGAGACTTATTAAATACTTTCACATTCTTATACTCTTAACCAAGAGGTTAGAAGTTCGAATCTTACACTTTGTCAAAAGAAAAAATATATATATGGAGTATTTTCGAAACAAAAATACATCGAGTATGGAGTGTATATGGAGTTGTATGTGAATTTCAAAATGAAATTAAATATATTAAAACGAGGGTTGATGTTGATGTAATAAAAAAAAAAAAAAAAAAAAAAGGTTGAAGAATAATAATAGAAAATCAAAATAAAAAGGATAAATCGGAACCCAGGCGCCCAGCCCAGCCCGACTCTTCCAGCCCAACAACTTTTCAAGAAACCATCCCACAGTCTCAATCTCACTCTTAGATTTCTCTCCCGTCTCTCCCGACCGAAGTCTGCAGCCTCCTTCCCCAATTGCTTACCCGAGTTCCCAACTGCAATCTTCAATCTCTCTCAGATCTTAGCCGAGATTTCGAACTAATTCCATTCATTCAAGGGGAAAAAGGAAACTACCTATTGATCTTTGGAATTTCGAAGTCCAAAAGAGTAAATACATGGGGCGGTGCCTAGTAGCTGGGCTCTGCTTATGGTTCTCTCTGACTATGGTGATTGCAGATGCAAATGAGTCTTCTTTGGGAGGTTTAATCTGGTCTTCTGCAAAAGAAGAGGGCGATCTCCTTATCGAGCACGGGCCCCAGGAAGATTCCGCCGTCGCTACACCCGTTCTTCACGACGCCGATGGCTTCGATGGCGGCTTTTCTTCCCTCGATAGCATGTTACAGTGGGCTATAGGTACTCTTATATTCATTATTACATTTCATGTTCGGCAATAAGGTTATACCATCAAGTATTAGCATTAGCAATCACAAGGATGACTGAAATTCATGCCACTGTACTTGGTGTAGTAGTTTGAGACGTCCTTCAAATTCATGCCAATCAGCGTTTTGTACTAACCTAAATTATACTTCTAGACTTGAAAGTATCTGTTTTCAAATTATAATGTGACATAATTTATACACTTCGATAAAGGATGAGTTTTGCAATGAAGAAGATGAGGGTAGGATGGGCGGAAATGTAACTCGGGAGTCTAAATTAGCGACGTTTGACAGAAAGTTGGTTGAATCCTTGTGCAGCTCTCTATCAGTGTAGGTTGGTCGGAACTGAAGACATGTGGAAGGGCAGGAGGGTTATTAACATTATGAAATGAAGGAAATGGAAGGTGGGGAATGAGCTAGAAGGTGTATTTTCTTTACTGAGTAAGTTGGAACTTGCGATGGCTCAATATTGTGGGTGTTCGATGTTTATTAACCCTGTAAAAGAAAGGATAAGAAGGAATCTTGTGGGAAATTGAAACTCCTGTATGGTTACTGTGAGGGTACAATGTTCAGATAGTATGAGAAGGTCTGATGACCTGATTCAGAGACAAGCAGTGGAAATTTTAAGTGGTGTAATTTTTGCATGCGCACACACTGTTCATTGCTTGTATGCTGGAGTCTCCTCTGTATTTTAAGTTTCACACCATTGTGATTTTGCATTTCATTGTTTTTGCATACTGTGTGAAACTTGCCATTGAACGATGTGATTTTTTATTCAAGTCTTTTAAGTTCTTTTACCAAATTGATTATTAAATGTTGAAGTTCCTCTAGTATATGTAATGGAGAGAGGGTTGATATGCCTCATATTTCTCCAAACCTTTGAACCAACTTCCTGATTATGTACTTGTCTGAATTAGGCCATTCTGATCCTGCAAAACTAAAGGATACTGCGCAAGATGTTAAGCAATTGTCTCCAAGTGAGCTAAAGAAGCATCAAGAAGAAATTAAGGTACAATTGTAATGTATTTCAGTTTCTGTATGGAAATTAATCAAAGAAATGGTCAAGAAATGAACATTTTGTATTGCTATGTATGTTCACTTTTGTAAATTTCATTATTTTAACGAAATCTTATTATTTAAAAAATATATATATTATTTATGTTATTCCCTTTTGTTATGGGTTGTCCAACTTTGAATACTTATTTATCTCATATATAGTACTCTGCTTTTGTAGGACCTAATAGAGAAGTTGAAGATGCCATCAGATGCAAAACTGATGCAAATTGCGATAGATGACTTGAAAAATTCCTCTCTATCATTGGAAGATCGCTATCGGGCATTACAAGAGCTTTTAATACTTGTTGAGCCAATAGATAATGCAAATGGTTAGTTCTAGCATCACTATTAGTGAGGATCCTTACAATGAATCATACAACTTCTTTCTTTTTATTTGTTGCTGTTGGCTGTGGCAATTAACTATGCTCCTATTACCTTATGAGTCGAGGGACCTGTGAATTTCTTCATTCAATTCCTTACACTTTATGTTACATGTTGAAAACACTTTAAAGAAGTTGAATTTCTTCTCTCAGAAGGCACTTACTCTCAAAGCAATTAAAATACATGTTAACATTCGTGAGTTATTCTTGAAAATTTGCTGCCATAGGACGTGAGGACTTAACTGTTTTAAGTTGCAGTGACTTGTTTAGTGCTTTTAACTGAGTTCATAATTGTATCCTACATTATTCTTAATGTTATTTTCGACTTTGTTATCTTGCCTTCTCTTACATTATTGAAATTTTGCGTTTCTTTTTCTAAAAAAAAAATCTTTTTATTTCATAGATTTGGACAAACTTGGGGGCCTTGTTGTGCTTATCCACGAGCTTAATCATATTGATCCTGATGTTAGGAAAGTTGCAGCATGGATTCTTGGAAAAGCCAGTCAAAATAACCCTATTGTTCAAAAGCAGGTACTATCATACACCGAGTGCAATTTTTTTTATAGATCTCTTTTTAGAATTTGTCTCCATCTGCATTCTTATTGCCAGGTATTGGAACTCGGGGCACTAGCAAAATTAGTAAGGATGGTAAAATCTGACTTCGTAGAAGAAGCCATCAAGGCATTGTATGCTATTTCATCCCTGGTTCGAAATAATTTATTTGGTCAGGAATTATTCTATGCTGAAGCTGGAGAAACTATGCTTCAGGTGATCTACTGAAGATTAGAAGGGGATTGATGTGCCACTTTCATGCTACAACCAGTTGAAGTGACGGTTTTCTTTTTGTTCCTATAGGATATTCTAAGCAACTCAAGCATGGATATCAGATTGCTAAAGAAAGTTGTGTTTCTAGCAAGTGATCTGGCCGAGACACAACTAGAAAGAGCAGATGAAGTGAGGCCATTTTTTGGAGATCGCCTTTTCTTGAAGTCCGTGGTTGATCTAACCCATTTGACAGACATTGATCTCCAGGAGAAGGTGGGTTTTTTTTTTTTTTTTTTTTTTCTGAAATCCTTCTGCATTTTAATTTATATTATTATTTCAGTGCGTTAACAGGAGCTTCCTTTTAGATTAATCTTCATTTTTAGAAGAAGCCATCAAGGCATCGTGTTATTGTTGTTTCGTCAAGATGTCTGTTTACTACATTATTCCAAAGTTTCTTTGACATATTGTGTAAGGTAGGAGGTATGGCCCTTATTCAAATGAAAGGTCTTCGTATGCATTCACAGGCTCTTATTACTCTAAAGAACCTCCTGCTTCTGAGGACAACAAAAGCTCAAGTTTTAAGAGATTTTTGTGGGTTGGACGTTGCTTTGGAGAGGATGAGGATGAAGCTGGAGGTATTAATGGAAGAGGAAGATCACCGAGATTATGCAATGGATGTGGAAAATCTACGAAGCCAAGTGGAGTTCATTTTCCTTGAAAAGCTGGGAAAGGTAGTTTCTACTCTGAGATTAATCATTGTAATGGTGAGTTAG

mRNA sequence

ATGCGGTTGGAATCGAGAAGGAGAAGACTAAAGGCTGAAGATGCAAATGAGTCTTCTTTGGGAGGTTTAATCTGGTCTTCTGCAAAAGAAGAGGGCGATCTCCTTATCGAGCACGGGCCCCAGGAAGATTCCGCCGTCGCTACACCCGTTCTTCACGACGCCGATGGCTTCGATGGCGGCTTTTCTTCCCTCGATAGCATGTTACAGTGGGCTATAGGCCATTCTGATCCTGCAAAACTAAAGGATACTGCGCAAGATGTTAAGCAATTGTCTCCAAGTGAGCTAAAGAAGCATCAAGAAGAAATTAAGGACCTAATAGAGAAGTTGAAGATGCCATCAGATGCAAAACTGATGCAAATTGCGATAGATGACTTGAAAAATTCCTCTCTATCATTGGAAGATCGCTATCGGGCATTACAAGAGCTTTTAATACTTGTTGAGCCAATAGATAATGCAAATGATTTGGACAAACTTGGGGGCCTTGTTGTGCTTATCCACGAGCTTAATCATATTGATCCTGATGTTAGGAAAGTTGCAGCATGGATTCTTGGAAAAGCCAGTCAAAATAACCCTATTGTTCAAAAGCAGGTATTGGAACTCGGGGCACTAGCAAAATTAGTAAGGATGGTAAAATCTGACTTCGTAGAAGAAGCCATCAAGGCATTGTATGCTATTTCATCCCTGGTTCGAAATAATTTATTTGGTCAGGAATTATTCTATGCTGAAGCTGGAGAAACTATGCTTCAGGATATTCTAAGCAACTCAAGCATGGATATCAGATTGCTAAAGAAAGTTGTGTTTCTAGCAAGTGATCTGGCCGAGACACAACTAGAAAGAGCAGATGAAGTGAGGCCATTTTTTGGAGATCGCCTTTTCTTGAAGTCCGTGGTTGATCTAACCCATTTGACAGACATTGATCTCCAGGAGAAGGTAGGAGGTATGGCCCTTATTCAAATGAAAGGTCTTCGTATGCATTCACAGGCTCTTATTACTCTAAAGAACCTCCTGCTTCTGAGGACAACAAAAGCTCAAGTTTTAAGAGATTTTTGTGGGTTGGACGTTGCTTTGGAGAGGATGAGGATGAAGCTGGAGGTATTAATGGAAGAGGAAGATCACCGAGATTATGCAATGGATGTGGAAAATCTACGAAGCCAAGTGGAGTTCATTTTCCTTGAAAAGCTGGGAAAGGTAGTTTCTACTCTGAGATTAATCATTGTAATGGTGAGTTAG

Coding sequence (CDS)

ATGCGGTTGGAATCGAGAAGGAGAAGACTAAAGGCTGAAGATGCAAATGAGTCTTCTTTGGGAGGTTTAATCTGGTCTTCTGCAAAAGAAGAGGGCGATCTCCTTATCGAGCACGGGCCCCAGGAAGATTCCGCCGTCGCTACACCCGTTCTTCACGACGCCGATGGCTTCGATGGCGGCTTTTCTTCCCTCGATAGCATGTTACAGTGGGCTATAGGCCATTCTGATCCTGCAAAACTAAAGGATACTGCGCAAGATGTTAAGCAATTGTCTCCAAGTGAGCTAAAGAAGCATCAAGAAGAAATTAAGGACCTAATAGAGAAGTTGAAGATGCCATCAGATGCAAAACTGATGCAAATTGCGATAGATGACTTGAAAAATTCCTCTCTATCATTGGAAGATCGCTATCGGGCATTACAAGAGCTTTTAATACTTGTTGAGCCAATAGATAATGCAAATGATTTGGACAAACTTGGGGGCCTTGTTGTGCTTATCCACGAGCTTAATCATATTGATCCTGATGTTAGGAAAGTTGCAGCATGGATTCTTGGAAAAGCCAGTCAAAATAACCCTATTGTTCAAAAGCAGGTATTGGAACTCGGGGCACTAGCAAAATTAGTAAGGATGGTAAAATCTGACTTCGTAGAAGAAGCCATCAAGGCATTGTATGCTATTTCATCCCTGGTTCGAAATAATTTATTTGGTCAGGAATTATTCTATGCTGAAGCTGGAGAAACTATGCTTCAGGATATTCTAAGCAACTCAAGCATGGATATCAGATTGCTAAAGAAAGTTGTGTTTCTAGCAAGTGATCTGGCCGAGACACAACTAGAAAGAGCAGATGAAGTGAGGCCATTTTTTGGAGATCGCCTTTTCTTGAAGTCCGTGGTTGATCTAACCCATTTGACAGACATTGATCTCCAGGAGAAGGTAGGAGGTATGGCCCTTATTCAAATGAAAGGTCTTCGTATGCATTCACAGGCTCTTATTACTCTAAAGAACCTCCTGCTTCTGAGGACAACAAAAGCTCAAGTTTTAAGAGATTTTTGTGGGTTGGACGTTGCTTTGGAGAGGATGAGGATGAAGCTGGAGGTATTAATGGAAGAGGAAGATCACCGAGATTATGCAATGGATGTGGAAAATCTACGAAGCCAAGTGGAGTTCATTTTCCTTGAAAAGCTGGGAAAGGTAGTTTCTACTCTGAGATTAATCATTGTAATGGTGAGTTAG

Protein sequence

MRLESRRRRLKAEDANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIGHSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLEDRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIVQKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILSNSSMDIRLLKKVVFLASDLAETQLERADEVRPFFGDRLFLKSVVDLTHLTDIDLQEKVGGMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDHRDYAMDVENLRSQVEFIFLEKLGKVVSTLRLIIVMVS

Homology

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BLAST of Lag0002212 vs. NCBI nr
Match: KAG7025239.1 (FES1-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 604.7 bits (1558), Expect = 5.7e-169
Identity = 339/451 (75.17%), Postives = 361/451 (80.04%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIG 73
           D N+SS GGLIWSSAKEEGDLLI++ P+EDS  A PV+HDADGFDGGFSSLDSMLQWAIG
Sbjct: 21  DENKSSAGGLIWSSAKEEGDLLIQYRPEEDSTAAAPVVHDADGFDGGFSSLDSMLQWAIG 80

Query: 74  HSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLE 133
           HS+P KLK+TAQDVKQLSPSELKK +EEIKDL+EKLKMPSDAKLMQIAIDDLKNSSLSLE
Sbjct: 81  HSNPEKLKETAQDVKQLSPSELKKRREEIKDLMEKLKMPSDAKLMQIAIDDLKNSSLSLE 140

Query: 134 DRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIV 193
           DRYRALQELLILVEPIDNANDL KLGGL VLIHELNHIDP+VRKVAAWILGKASQNNPIV
Sbjct: 141 DRYRALQELLILVEPIDNANDLGKLGGLAVLIHELNHIDPEVRKVAAWILGKASQNNPIV 200

Query: 194 QK--------------------------QVLELGALAKLVRMVKSDFVEEAIKALYAISS 253
           QK                          QVLELGAL+KLVRM KSDFVEEAIKA YAISS
Sbjct: 201 QKQVLPYAEWNYSYRPLCRICLQLHSYCQVLELGALSKLVRMAKSDFVEEAIKAFYAISS 260

Query: 254 LVRNNLFGQELFYAEAGETMLQDILSNSSMDIRLLKKVVFLASDLAETQLERADEV-RPF 313
           LVRNNL GQELFYA AGETMLQDIL NSSMDIRL KK VFL SDLAETQLE+A+E  R F
Sbjct: 261 LVRNNLSGQELFYAAAGETMLQDILRNSSMDIRLQKKAVFLVSDLAETQLEKANEAERSF 320

Query: 314 FGDRLFLKSVVDLTHLTDIDLQEKV---------------------------------GG 373
           FGDR FLKSVVDLTH  DIDLQEKV                                  G
Sbjct: 321 FGDRFFLKSVVDLTHSKDIDLQEKVEEAIKVLYYCCLAQDVCFLRIFQNLSDVLICKLRG 380

Query: 374 MALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDHR 405
           +ALIQM+G  ++ QALI LKNLLLLRTTKAQVLR+FCGLDVALERMR+KLEVLMEEEDHR
Sbjct: 381 VALIQMEGFDVNLQALIALKNLLLLRTTKAQVLREFCGLDVALERMRVKLEVLMEEEDHR 440

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BLAST of Lag0002212 vs. NCBI nr
Match: XP_023513988.1 (hsp70 nucleotide exchange factor FES1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602.1 bits (1551), Expect = 3.7e-168
Identity = 324/384 (84.38%), Postives = 340/384 (88.54%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIG 73
           D N+SS GGLIWSSAKEEGDLLI++ P+EDS  A PV+HDADGFDGGFSSLDSMLQWAIG
Sbjct: 21  DENKSSAGGLIWSSAKEEGDLLIQYRPEEDSTAAAPVVHDADGFDGGFSSLDSMLQWAIG 80

Query: 74  HSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLE 133
           HS+P KLK+TAQDVKQLSPSELKK QEEIKDL+EKLKMPSDAKLMQIAIDDLKNSSLSLE
Sbjct: 81  HSNPEKLKETAQDVKQLSPSELKKRQEEIKDLMEKLKMPSDAKLMQIAIDDLKNSSLSLE 140

Query: 134 DRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIV 193
           DRYRALQELLILVEPIDNANDL KLGGL VLIHELNHIDP+VRKVAAWILGKASQNNPIV
Sbjct: 141 DRYRALQELLILVEPIDNANDLGKLGGLAVLIHELNHIDPEVRKVAAWILGKASQNNPIV 200

Query: 194 QKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILS 253
           QKQVLELGAL+KLVRM KSDFVEEAIKA YAISSLVRNNL GQELFYA AGETMLQDIL 
Sbjct: 201 QKQVLELGALSKLVRMAKSDFVEEAIKAFYAISSLVRNNLSGQELFYAAAGETMLQDILR 260

Query: 254 NSSMDIRLLKKVVFLASDLAETQLERADEV-RPFFGDRLFLKSVVDLTHLTDIDLQEKVG 313
           NSSMDIRL KK VFL SDLAETQLE+ADE  R FFGDR FLKSVVDLTH  DIDLQEK  
Sbjct: 261 NSSMDIRLQKKAVFLVSDLAETQLEKADEAERSFFGDRFFLKSVVDLTHSKDIDLQEK-- 320

Query: 314 GMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDH 373
                          ALI LKNLLLLRTTKAQVLR+FCGLDVALERMR+KLEVLMEEEDH
Sbjct: 321 ---------------ALIALKNLLLLRTTKAQVLREFCGLDVALERMRVKLEVLMEEEDH 380

Query: 374 RDYAMDVENLRSQVEFIFLEKLGK 397
           RDYAMDVE+LR+QVE IF E+LGK
Sbjct: 381 RDYAMDVESLRNQVELIFHERLGK 387

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BLAST of Lag0002212 vs. NCBI nr
Match: XP_022960457.1 (hsp70 nucleotide exchange factor FES1 [Cucurbita moschata])

HSP 1 Score: 600.1 bits (1546), Expect = 1.4e-167
Identity = 323/384 (84.11%), Postives = 340/384 (88.54%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIG 73
           D N+SS GGLIWSSAKEEGDLLI++ P+EDS  A PV+HDADGFDGGFSSLDSMLQWAIG
Sbjct: 21  DENKSSAGGLIWSSAKEEGDLLIQYRPEEDSTAAAPVVHDADGFDGGFSSLDSMLQWAIG 80

Query: 74  HSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLE 133
           HS+P KLK+TAQDVKQLSPSELKK QEEIKDL+EKLKMPSDAKLMQIAIDDLKNSSLSLE
Sbjct: 81  HSNPEKLKETAQDVKQLSPSELKKRQEEIKDLMEKLKMPSDAKLMQIAIDDLKNSSLSLE 140

Query: 134 DRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIV 193
           DRYRALQELLILVEPIDNANDL KLGGL VLIHELNHIDP+VRKVAAWILGKASQNNPIV
Sbjct: 141 DRYRALQELLILVEPIDNANDLGKLGGLAVLIHELNHIDPEVRKVAAWILGKASQNNPIV 200

Query: 194 QKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILS 253
           QKQVLELGAL+KLVRM KSDFVEEAIKA YAISSLVRNNL GQELFYA AGETMLQDIL 
Sbjct: 201 QKQVLELGALSKLVRMAKSDFVEEAIKAFYAISSLVRNNLSGQELFYAAAGETMLQDILR 260

Query: 254 NSSMDIRLLKKVVFLASDLAETQLERADEV-RPFFGDRLFLKSVVDLTHLTDIDLQEKVG 313
           NSSMDIRL KK VFL SDLAETQLE+A+E  R FFGDR FLKSVVDLTH  DIDLQEK  
Sbjct: 261 NSSMDIRLQKKAVFLVSDLAETQLEKANEAERSFFGDRFFLKSVVDLTHSKDIDLQEK-- 320

Query: 314 GMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDH 373
                          ALI LKNLLLLRTTKAQVLR+FCGLDVALERMR+KLEVLMEEEDH
Sbjct: 321 ---------------ALIALKNLLLLRTTKAQVLREFCGLDVALERMRVKLEVLMEEEDH 380

Query: 374 RDYAMDVENLRSQVEFIFLEKLGK 397
           RDYAMDVE+LR+QVE IF E+LGK
Sbjct: 381 RDYAMDVESLRNQVELIFHERLGK 387

[Close]

BLAST of Lag0002212 vs. NCBI nr
Match: XP_023004240.1 (hsp70 nucleotide exchange factor FES1 [Cucurbita maxima])

HSP 1 Score: 597.8 bits (1540), Expect = 7.0e-167
Identity = 320/384 (83.33%), Postives = 339/384 (88.28%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIG 73
           D N+SS+G LIWSSAKEEGDLLI++ P+EDS  A PV HDADGFDGGFSSLDSMLQWAIG
Sbjct: 21  DENKSSVGSLIWSSAKEEGDLLIQYRPEEDSTAAAPVAHDADGFDGGFSSLDSMLQWAIG 80

Query: 74  HSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLE 133
           HS+P KLK+TAQDVKQLSPSELKK QEEIKDL+EKLKMPSDAKLMQIA+DDLKNSSLSLE
Sbjct: 81  HSNPEKLKETAQDVKQLSPSELKKRQEEIKDLMEKLKMPSDAKLMQIALDDLKNSSLSLE 140

Query: 134 DRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIV 193
           DRYRALQELLILVEPIDNANDL KLGGL VLIHELNHIDP+VRKVAAWILGKASQNNPIV
Sbjct: 141 DRYRALQELLILVEPIDNANDLGKLGGLAVLIHELNHIDPEVRKVAAWILGKASQNNPIV 200

Query: 194 QKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILS 253
           QKQVLELGAL+KLVRM KSDFVEEAIKA YAISSLVRNNL GQELFYA AGETMLQDIL 
Sbjct: 201 QKQVLELGALSKLVRMAKSDFVEEAIKAFYAISSLVRNNLSGQELFYAAAGETMLQDILR 260

Query: 254 NSSMDIRLLKKVVFLASDLAETQLERADEV-RPFFGDRLFLKSVVDLTHLTDIDLQEKVG 313
           NSSMDIRL KK VFL SDLAETQLE+ADE  R FFGDR FLKSVVDLTH  DIDLQEK  
Sbjct: 261 NSSMDIRLQKKAVFLVSDLAETQLEKADEAERSFFGDRFFLKSVVDLTHSKDIDLQEK-- 320

Query: 314 GMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDH 373
                          ALI +KNLLLLRTTKAQVLR+FCGLD+ALERMR+KLEVLMEEEDH
Sbjct: 321 ---------------ALIAIKNLLLLRTTKAQVLREFCGLDIALERMRVKLEVLMEEEDH 380

Query: 374 RDYAMDVENLRSQVEFIFLEKLGK 397
           RDYAMDVE+LR+QVE IF E+LGK
Sbjct: 381 RDYAMDVESLRNQVELIFHERLGK 387

[Close]

BLAST of Lag0002212 vs. NCBI nr
Match: XP_022148873.1 (nucleotide exchange factor SIL1 isoform X1 [Momordica charantia])

HSP 1 Score: 595.5 bits (1534), Expect = 3.5e-166
Identity = 317/384 (82.55%), Postives = 342/384 (89.06%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIG 73
           + N+SSLGGLIWSSAKEEGDLL+E+ PQEDS  AT V+HD DGFDGGF SLDSMLQWAIG
Sbjct: 44  NTNKSSLGGLIWSSAKEEGDLLVENRPQEDSTAATAVVHDPDGFDGGFYSLDSMLQWAIG 103

Query: 74  HSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLE 133
           HSDPAKLK TAQDVKQLSPS LKK QEEIKDL+EKLKMPSDAKL+QIAIDDLKNSSLSLE
Sbjct: 104 HSDPAKLKGTAQDVKQLSPSALKKRQEEIKDLMEKLKMPSDAKLIQIAIDDLKNSSLSLE 163

Query: 134 DRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIV 193
           D +RALQELL+LVEPIDNANDL+KLGG+ VLIHELNHIDP+ RKVAAWILGKASQNNPIV
Sbjct: 164 DHHRALQELLVLVEPIDNANDLNKLGGIAVLIHELNHIDPNARKVAAWILGKASQNNPIV 223

Query: 194 QKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILS 253
           QKQVLELGALAKLV MVKSDFVEEAIKALYAIS+L+RNNL GQELFYAE+G+++LQDILS
Sbjct: 224 QKQVLELGALAKLVTMVKSDFVEEAIKALYAISALIRNNLSGQELFYAESGDSLLQDILS 283

Query: 254 NSSMDIRLLKKVVFLASDLAETQLERADEVR-PFFGDRLFLKSVVDLTHLTDIDLQEKVG 313
           NSSMDIRL KK VFL SDLAETQLE+ADE   PF GDRLFLKSVVDLTH TDIDLQEK  
Sbjct: 284 NSSMDIRLKKKAVFLTSDLAETQLEKADEAEMPFLGDRLFLKSVVDLTHSTDIDLQEK-- 343

Query: 314 GMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDH 373
                          AL+ LKNLLLLRTT+AQVLR+FCGLDVAL+RMR+KLEVLMEEEDH
Sbjct: 344 ---------------ALVALKNLLLLRTTEAQVLREFCGLDVALQRMRVKLEVLMEEEDH 403

Query: 374 RDYAMDVENLRSQVEFIFLEKLGK 397
           RDYAMDVE+LRSQVE IFLEKLGK
Sbjct: 404 RDYAMDVESLRSQVELIFLEKLGK 410

[Close]

BLAST of Lag0002212 vs. ExPASy Swiss-Prot
Match: Q32KV6 (Nucleotide exchange factor SIL1 OS=Bos taurus OX=9913 GN=SIL1 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 9.5e-18
Identity = 67/185 (36.22%), Postives = 109/185 (58.92%), Query Frame = 0


Query: 94  ELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLEDRYRALQELLILVEPIDNAN 153
           ELKK  EE+  +IE     +D ++M   I+   +SS SLE++  AL +L   V  +DNA 
Sbjct: 167 ELKKDFEELNVVIE-----TDMQIMVRLINKFNSSSSSLEEKIAALFDLEYYVHQMDNAQ 226

Query: 154 DLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIVQKQVLELGALAKLVRMVKSD 213
           DL   GGL V+I+ LN  +P V++ AA++LG A  +NP VQ + +E GAL KL+ ++ ++
Sbjct: 227 DLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVILATE 286

Query: 214 F-VEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILSNSSMDIRLLKKVVFLASDL 273
             +    KAL+A+ SL+R+  + Q+ F    G  +L+ ++    M++ L  +VV L  DL
Sbjct: 287 QPLTTKKKALFALCSLLRHFPYAQQQFLKLGGLQVLRSLVQEKGMEV-LAVRVVTLLYDL 345

Query: 274 AETQL 278
              ++
Sbjct: 347 VTEKM 345

[Close]

BLAST of Lag0002212 vs. ExPASy Swiss-Prot
Match: Q1E3S4 (Hsp70 nucleotide exchange factor FES1 OS=Coccidioides immitis (strain RS) OX=246410 GN=FES1 PE=3 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 1.6e-17
Identity = 59/172 (34.30%), Postives = 102/172 (59.30%), Query Frame = 0


Query: 62  SSLDSMLQWAIGHSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIA 121
           S ++++L+W+I +S PA+  D  Q  ++ S   L    + ++ L+     PSDA LM+ A
Sbjct: 3   SHMNNLLKWSIENSVPAQPDDPEQVKQERSLDRL--DTQALQRLLS--NAPSDADLMKAA 62

Query: 122 IDDLKNSSLSLEDRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAW 181
           ++ + +   +LE++  A      L+E +DNAN++  LG    L+  L+  +P +RK+AAW
Sbjct: 63  MEVVSDDFATLENKLIAFDNFEQLIENLDNANNMGVLGLWTPLVEALSDAEPQMRKMAAW 122

Query: 182 ILGKASQNNPIVQKQVLELGALAKLVRMVKSD-FVEEAIKALYAISSLVRNN 233
            +G A QNN + Q ++L+  A+ KL+ + K+D       KA+YA+SS VRN+
Sbjct: 123 CIGTAVQNNEMAQNKLLDFKAVPKLLSLAKTDPDTTVRRKAIYALSSAVRNH 170

[Close]

BLAST of Lag0002212 vs. ExPASy Swiss-Prot
Match: A2R4I6 (Hsp70 nucleotide exchange factor fes1 OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=fes1 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.8e-17
Identity = 52/121 (42.98%), Postives = 77/121 (63.64%), Query Frame = 0


Query: 112 PSDAKLMQIAIDDLKNSSLSLEDRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHI 171
           PS+A LM+ A++ L++  + LE++  A      L+E IDNAN+L+ LG    L+  L+H 
Sbjct: 55  PSEADLMKAAMEALRSDEVDLENKLIAFDNFEQLIESIDNANNLEPLGLWTPLVELLDHK 114

Query: 172 DPDVRKVAAWILGKASQNNPIVQKQVLELGALAKLVRMVKSDFVEEA-IKALYAISSLVR 231
           +PD+R++AAW +G A QNN   Q +++ L AL KLV +  +D       KA+YAISS VR
Sbjct: 115 EPDMRRMAAWCIGTAVQNNEKAQDKLIVLNALPKLVSLATADTTPVVRKKAVYAISSAVR 174

[Close]

BLAST of Lag0002212 vs. ExPASy Swiss-Prot
Match: Q6P6S4 (Nucleotide exchange factor SIL1 OS=Rattus norvegicus OX=10116 GN=Sil1 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.1e-16
Identity = 73/208 (35.10%), Postives = 115/208 (55.29%), Query Frame = 0


Query: 87  VKQL--SPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLEDRYRALQELLI 146
           VKQL     ELKK  +E+  ++E     +D ++M   I+   +SS SLE++  AL +L  
Sbjct: 161 VKQLFRPIEELKKEFDELNVVLE-----TDMQIMVRLINKFNSSSSSLEEKVAALFDLEY 220

Query: 147 LVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIVQKQVLELGALA 206
            V  +DNA DL   GGL V+I+ LN  +P V++ AA++LG A  +NP VQ + +E GAL 
Sbjct: 221 YVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQ 280

Query: 207 KLVRMVKSDF-VEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILSNSSMDIRLLK 266
           KL+ ++ ++  +    K L+A+ SL+R+  + Q+ F    G  +L+ ++   S  + L  
Sbjct: 281 KLLVILATEQPLPAKKKVLFALCSLLRHFPYAQQQFLKLGGLQVLRSLVQEKSAKV-LAV 340

Query: 267 KVVFLASDL------AETQLERADEVRP 286
           +VV L  DL      AE + E   E  P
Sbjct: 341 RVVTLLYDLVTEKMFAEEEAELTQESSP 362

[Close]

BLAST of Lag0002212 vs. ExPASy Swiss-Prot
Match: Q9H173 (Nucleotide exchange factor SIL1 OS=Homo sapiens OX=9606 GN=SIL1 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.4e-16
Identity = 77/227 (33.92%), Postives = 121/227 (53.30%), Query Frame = 0


Query: 78  AKLKDTAQDVKQLSPSELKKHQEEIKDL---IEKLK---------MPSDAKLMQIAIDDL 137
           AK K+ A+     S  E K  Q E+K L   IE+LK         + +D ++M   I+  
Sbjct: 136 AKFKEGAE---MESSKEDKARQAEVKRLFRPIEELKKDFDELNVVIETDMQIMVRLINKF 195

Query: 138 KNSSLSLEDRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGK 197
            +SS SLE++  AL +L   V  +DNA DL   GGL V+I+ LN  +P V++ AA++LG 
Sbjct: 196 NSSSSSLEEKIAALFDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGA 255

Query: 198 ASQNNPIVQKQVLELGALAKLVRMVKSDF-VEEAIKALYAISSLVRNNLFGQELFYAEAG 257
           A  +NP VQ + +E GAL KL+ ++ ++  +    K L+A+ SL+R+  + Q  F    G
Sbjct: 256 AFSSNPKVQVEAIEGGALQKLLVILATEQPLTAKKKVLFALCSLLRHFPYAQRQFLKLGG 315

Query: 258 ETMLQDILSNSSMDIRLLKKVVFLASDL------AETQLERADEVRP 286
             +L+ ++     ++ L  +VV L  DL      AE + E   E+ P
Sbjct: 316 LQVLRTLVQEKGTEV-LAVRVVTLLYDLVTEKMFAEEEAELTQEMSP 358

[Close]

BLAST of Lag0002212 vs. ExPASy TrEMBL
Match: A0A6J1H7G4 (hsp70 nucleotide exchange factor FES1 OS=Cucurbita moschata OX=3662 GN=LOC111461185 PE=4 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 6.8e-168
Identity = 323/384 (84.11%), Postives = 340/384 (88.54%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIG 73
           D N+SS GGLIWSSAKEEGDLLI++ P+EDS  A PV+HDADGFDGGFSSLDSMLQWAIG
Sbjct: 21  DENKSSAGGLIWSSAKEEGDLLIQYRPEEDSTAAAPVVHDADGFDGGFSSLDSMLQWAIG 80

Query: 74  HSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLE 133
           HS+P KLK+TAQDVKQLSPSELKK QEEIKDL+EKLKMPSDAKLMQIAIDDLKNSSLSLE
Sbjct: 81  HSNPEKLKETAQDVKQLSPSELKKRQEEIKDLMEKLKMPSDAKLMQIAIDDLKNSSLSLE 140

Query: 134 DRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIV 193
           DRYRALQELLILVEPIDNANDL KLGGL VLIHELNHIDP+VRKVAAWILGKASQNNPIV
Sbjct: 141 DRYRALQELLILVEPIDNANDLGKLGGLAVLIHELNHIDPEVRKVAAWILGKASQNNPIV 200

Query: 194 QKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILS 253
           QKQVLELGAL+KLVRM KSDFVEEAIKA YAISSLVRNNL GQELFYA AGETMLQDIL 
Sbjct: 201 QKQVLELGALSKLVRMAKSDFVEEAIKAFYAISSLVRNNLSGQELFYAAAGETMLQDILR 260

Query: 254 NSSMDIRLLKKVVFLASDLAETQLERADEV-RPFFGDRLFLKSVVDLTHLTDIDLQEKVG 313
           NSSMDIRL KK VFL SDLAETQLE+A+E  R FFGDR FLKSVVDLTH  DIDLQEK  
Sbjct: 261 NSSMDIRLQKKAVFLVSDLAETQLEKANEAERSFFGDRFFLKSVVDLTHSKDIDLQEK-- 320

Query: 314 GMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDH 373
                          ALI LKNLLLLRTTKAQVLR+FCGLDVALERMR+KLEVLMEEEDH
Sbjct: 321 ---------------ALIALKNLLLLRTTKAQVLREFCGLDVALERMRVKLEVLMEEEDH 380

Query: 374 RDYAMDVENLRSQVEFIFLEKLGK 397
           RDYAMDVE+LR+QVE IF E+LGK
Sbjct: 381 RDYAMDVESLRNQVELIFHERLGK 387

[Close]

BLAST of Lag0002212 vs. ExPASy TrEMBL
Match: A0A6J1KU13 (hsp70 nucleotide exchange factor FES1 OS=Cucurbita maxima OX=3661 GN=LOC111497634 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 3.4e-167
Identity = 320/384 (83.33%), Postives = 339/384 (88.28%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIG 73
           D N+SS+G LIWSSAKEEGDLLI++ P+EDS  A PV HDADGFDGGFSSLDSMLQWAIG
Sbjct: 21  DENKSSVGSLIWSSAKEEGDLLIQYRPEEDSTAAAPVAHDADGFDGGFSSLDSMLQWAIG 80

Query: 74  HSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLE 133
           HS+P KLK+TAQDVKQLSPSELKK QEEIKDL+EKLKMPSDAKLMQIA+DDLKNSSLSLE
Sbjct: 81  HSNPEKLKETAQDVKQLSPSELKKRQEEIKDLMEKLKMPSDAKLMQIALDDLKNSSLSLE 140

Query: 134 DRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIV 193
           DRYRALQELLILVEPIDNANDL KLGGL VLIHELNHIDP+VRKVAAWILGKASQNNPIV
Sbjct: 141 DRYRALQELLILVEPIDNANDLGKLGGLAVLIHELNHIDPEVRKVAAWILGKASQNNPIV 200

Query: 194 QKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILS 253
           QKQVLELGAL+KLVRM KSDFVEEAIKA YAISSLVRNNL GQELFYA AGETMLQDIL 
Sbjct: 201 QKQVLELGALSKLVRMAKSDFVEEAIKAFYAISSLVRNNLSGQELFYAAAGETMLQDILR 260

Query: 254 NSSMDIRLLKKVVFLASDLAETQLERADEV-RPFFGDRLFLKSVVDLTHLTDIDLQEKVG 313
           NSSMDIRL KK VFL SDLAETQLE+ADE  R FFGDR FLKSVVDLTH  DIDLQEK  
Sbjct: 261 NSSMDIRLQKKAVFLVSDLAETQLEKADEAERSFFGDRFFLKSVVDLTHSKDIDLQEK-- 320

Query: 314 GMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDH 373
                          ALI +KNLLLLRTTKAQVLR+FCGLD+ALERMR+KLEVLMEEEDH
Sbjct: 321 ---------------ALIAIKNLLLLRTTKAQVLREFCGLDIALERMRVKLEVLMEEEDH 380

Query: 374 RDYAMDVENLRSQVEFIFLEKLGK 397
           RDYAMDVE+LR+QVE IF E+LGK
Sbjct: 381 RDYAMDVESLRNQVELIFHERLGK 387

[Close]

BLAST of Lag0002212 vs. ExPASy TrEMBL
Match: A0A6J1D5A9 (nucleotide exchange factor SIL1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017440 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.7e-166
Identity = 317/384 (82.55%), Postives = 342/384 (89.06%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIG 73
           + N+SSLGGLIWSSAKEEGDLL+E+ PQEDS  AT V+HD DGFDGGF SLDSMLQWAIG
Sbjct: 44  NTNKSSLGGLIWSSAKEEGDLLVENRPQEDSTAATAVVHDPDGFDGGFYSLDSMLQWAIG 103

Query: 74  HSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLE 133
           HSDPAKLK TAQDVKQLSPS LKK QEEIKDL+EKLKMPSDAKL+QIAIDDLKNSSLSLE
Sbjct: 104 HSDPAKLKGTAQDVKQLSPSALKKRQEEIKDLMEKLKMPSDAKLIQIAIDDLKNSSLSLE 163

Query: 134 DRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIV 193
           D +RALQELL+LVEPIDNANDL+KLGG+ VLIHELNHIDP+ RKVAAWILGKASQNNPIV
Sbjct: 164 DHHRALQELLVLVEPIDNANDLNKLGGIAVLIHELNHIDPNARKVAAWILGKASQNNPIV 223

Query: 194 QKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILS 253
           QKQVLELGALAKLV MVKSDFVEEAIKALYAIS+L+RNNL GQELFYAE+G+++LQDILS
Sbjct: 224 QKQVLELGALAKLVTMVKSDFVEEAIKALYAISALIRNNLSGQELFYAESGDSLLQDILS 283

Query: 254 NSSMDIRLLKKVVFLASDLAETQLERADEVR-PFFGDRLFLKSVVDLTHLTDIDLQEKVG 313
           NSSMDIRL KK VFL SDLAETQLE+ADE   PF GDRLFLKSVVDLTH TDIDLQEK  
Sbjct: 284 NSSMDIRLKKKAVFLTSDLAETQLEKADEAEMPFLGDRLFLKSVVDLTHSTDIDLQEK-- 343

Query: 314 GMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDH 373
                          AL+ LKNLLLLRTT+AQVLR+FCGLDVAL+RMR+KLEVLMEEEDH
Sbjct: 344 ---------------ALVALKNLLLLRTTEAQVLREFCGLDVALQRMRVKLEVLMEEEDH 403

Query: 374 RDYAMDVENLRSQVEFIFLEKLGK 397
           RDYAMDVE+LRSQVE IFLEKLGK
Sbjct: 404 RDYAMDVESLRSQVELIFLEKLGK 410

[Close]

BLAST of Lag0002212 vs. ExPASy TrEMBL
Match: A0A0A0KA97 (Fes1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G089250 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.7e-166
Identity = 320/386 (82.90%), Postives = 341/386 (88.34%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDS--AVATPVLHDADGFDGGFSSLDSMLQWA 73
           DAN+SSL GLIWSSAK+EGDLLI+H PQEDS  A A  V+HDADGFDGGFSSLDSMLQWA
Sbjct: 21  DANDSSLEGLIWSSAKQEGDLLIDHRPQEDSSAAAAASVVHDADGFDGGFSSLDSMLQWA 80

Query: 74  IGHSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLS 133
           IGHSDPAKLKDTAQDVK+LSP+E+KK QEEIKDLIE+LK+PSDAKLMQIA+DDLKNSSLS
Sbjct: 81  IGHSDPAKLKDTAQDVKRLSPNEIKKRQEEIKDLIEELKLPSDAKLMQIAVDDLKNSSLS 140

Query: 134 LEDRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNP 193
           LEDR+RALQELL+LVEPIDNANDLDKLGGL VL  ELNH+DPDVRK+AAWILGKASQNNP
Sbjct: 141 LEDRHRALQELLVLVEPIDNANDLDKLGGLAVLTRELNHVDPDVRKIAAWILGKASQNNP 200

Query: 194 IVQKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDI 253
           IVQKQVLELGALAKLV M KSDFVEEAIKALYAISSLV+NNL GQELFYAEAGE MLQDI
Sbjct: 201 IVQKQVLELGALAKLVSMAKSDFVEEAIKALYAISSLVQNNLSGQELFYAEAGEIMLQDI 260

Query: 254 LSNSSMDIRLLKKVVFLASDLAETQLERADEV-RPFFGDRLFLKSVVDLTHLTDIDLQEK 313
           LSNSSMDIRL KK VFL SDLA TQLE+ DE  RPFFGDRLFLKSVV+L H TDIDLQEK
Sbjct: 261 LSNSSMDIRLQKKAVFLVSDLAVTQLEKPDEAERPFFGDRLFLKSVVNLIHSTDIDLQEK 320

Query: 314 VGGMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEE 373
                            ALI LKNLLLLRTTKAQVL++FCGLD ALERMRMKL+VLMEEE
Sbjct: 321 -----------------ALIALKNLLLLRTTKAQVLKEFCGLDAALERMRMKLKVLMEEE 380

Query: 374 DHRDYAMDVENLRSQVEFIFLEKLGK 397
           DHRDYAMDVE+LRSQVE  FLEKLGK
Sbjct: 381 DHRDYAMDVEDLRSQVELTFLEKLGK 389

[Close]

BLAST of Lag0002212 vs. ExPASy TrEMBL
Match: A0A1S3CBB7 (hsp70 nucleotide exchange factor FES1 OS=Cucumis melo OX=3656 GN=LOC103498900 PE=4 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 6.2e-161
Identity = 314/385 (81.56%), Postives = 334/385 (86.75%), Query Frame = 0


Query: 14  DANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWAIG 73
           DAN+SSL GLIWSSAKE           + +A A PV+HDADGFDGGFSSLDSMLQWAIG
Sbjct: 21  DANDSSLEGLIWSSAKE-----------DSAAAAAPVVHDADGFDGGFSSLDSMLQWAIG 80

Query: 74  HSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLE 133
           HSDPAKLKDTAQDVKQLSPSE+KK QEEIKDLIE+LK+PSDAKLMQIA+DDLKNSSLSLE
Sbjct: 81  HSDPAKLKDTAQDVKQLSPSEIKKRQEEIKDLIEELKLPSDAKLMQIAVDDLKNSSLSLE 140

Query: 134 DRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIV 193
           DR+RALQELL+LVEPIDNANDLDKLGGL VL  ELNH+DPDVRK+AAWILGKASQNNPIV
Sbjct: 141 DRHRALQELLVLVEPIDNANDLDKLGGLAVLTRELNHVDPDVRKIAAWILGKASQNNPIV 200

Query: 194 QKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDILS 253
           QKQVLELGALAKLV MVKSDFVEEAIKALYAISSLV+NNL GQ+LFYAEAGETMLQDILS
Sbjct: 201 QKQVLELGALAKLVSMVKSDFVEEAIKALYAISSLVQNNLSGQKLFYAEAGETMLQDILS 260

Query: 254 NSSMDIRLLKKVVFLASDLAETQLERADEV-RPFFGDRLFLKSVVDLTHLTDIDLQEKVG 313
           NSSMDIRL KK VFLASDL  TQLE+ DE  RPFFGDR FLKSVV+L H TDIDLQEK  
Sbjct: 261 NSSMDIRLQKKAVFLASDLTVTQLEKPDEAERPFFGDRFFLKSVVNLIHSTDIDLQEK-- 320

Query: 314 GMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEEDH 373
                          ALI LKNLLLLRTTKAQVL++FCGLD ALERMRMKL+VLMEEEDH
Sbjct: 321 ---------------ALIALKNLLLLRTTKAQVLKEFCGLDAALERMRMKLKVLMEEEDH 377

Query: 374 RDYAMDVENLRSQVEFIFLEKLGKV 398
           RDYAMDVE+LRSQVE IFLEKLGKV
Sbjct: 381 RDYAMDVEDLRSQVEQIFLEKLGKV 377

[Close]

BLAST of Lag0002212 vs. TAIR 10
Match: AT3G51980.1 (ARM repeat superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.3e-112
Identity = 222/385 (57.66%), Postives = 286/385 (74.29%), Query Frame = 0


Query: 12  AEDANESSLGGLIWSSAKEEGDLLIEHGPQEDSAVATPVLHDADGFDGGFSSLDSMLQWA 71
           AE  N S  GG++WSS ++E +L+      EDS V   V+ + D  DGGFSSLD ML WA
Sbjct: 24  AEKVNSS--GGMVWSSVRDEAELV------EDSGV---VIGEQDQIDGGFSSLDGMLHWA 83

Query: 72  IGHSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLS 131
           IGHSDPA LK+ A+D +++S  EL+K Q E+K+L+EKLKMPS+AKLMQIAIDDL NSSLS
Sbjct: 84  IGHSDPATLKEAAKDAEKMSLDELQKRQLELKELVEKLKMPSNAKLMQIAIDDLNNSSLS 143

Query: 132 LEDRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILGKASQNNP 191
           LEDR+RALQELLILVEPIDNANDL K GGL V+  ELNH D +VRK+AAW+LGKASQNNP
Sbjct: 144 LEDRHRALQELLILVEPIDNANDLSKSGGLRVVAGELNHDDTEVRKLAAWVLGKASQNNP 203

Query: 192 IVQKQVLELGALAKLVRMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAEAGETMLQDI 251
            VQ+QVLELGAL  L++MV S   EEA+KAL+A+S+L+RNN+ GQ+LF+A  G  ML+D+
Sbjct: 204 FVQEQVLELGALTTLIKMVNSSSTEEAVKALFAVSALIRNNIAGQDLFFAAHGYIMLRDV 263

Query: 252 LSNSSMDIRLLKKVVFLASDLAETQLERAD-EVRPFFGDRLFLKSVVDLTHLTDIDLQEK 311
           ++N S+D++L +K VFL  DLAE+QL+  + +  P F DRLFLKSVVDL  + D+DLQEK
Sbjct: 264 MNNGSLDMKLRRKAVFLVGDLAESQLQNTEKDELPIFKDRLFLKSVVDLIVVLDLDLQEK 323

Query: 312 VGGMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRDFCGLDVALERMRMKLEVLMEEE 371
                            AL  ++ LL L++ + QVL++ CGL+ ALERM+++LE  M +E
Sbjct: 324 -----------------ALTAIQTLLQLKSIEPQVLKESCGLEEALERMKLQLEESMADE 380

Query: 372 DHRDYAMDVENLRSQVEFIFLEKLG 396
             RDYA DVE++R +VE IF +KLG
Sbjct: 384 YKRDYAADVESIRGEVELIFRQKLG 380

[Close]

BLAST of Lag0002212 vs. TAIR 10
Match: AT3G09350.1 (Fes1A )

HSP 1 Score: 81.6 bits (200), Expect = 1.6e-15
Identity = 76/250 (30.40%), Postives = 122/250 (48.80%), Query Frame = 0


Query: 65  DSMLQWAIGHSDPAKLKDTAQDVKQLSPSELKKHQEEIK----DLIEKLKMPSDAKLMQI 124
           D +L+W++ H+D  +        +QLS  + K   E ++    D+++++K      +MQ 
Sbjct: 9   DGLLKWSLSHADGTR------PTRQLSEEDRKWFMEAMQSQTVDVVKRMK--EITLVMQT 68

Query: 125 AIDDLKNSSLSLEDRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAA 184
               L    ++ ED    L EL   VE ID ANDL  +GGLV L+  L +   ++R  AA
Sbjct: 69  PEQVLVEHGVTPEDIQDLLDELQEHVESIDMANDLHSIGGLVPLLSFLKNSHANIRAKAA 128

Query: 185 WILGKASQNNPIVQKQVLELGALAKLVRMVKSDF-VEEAIKALYAISSLVRNNLFGQELF 244
            ++    QNNP  Q+ V+E  AL  L+    SD  +    +AL AISSL+R+N  G   F
Sbjct: 129 DVVSTIVQNNPRSQELVMETNALESLLSNFTSDTDIHARTQALGAISSLIRHNKPGVTAF 188

Query: 245 YAEAGETMLQDILSNSSMDIRLLKKVVFLASDLAETQLERADEVRPFFGDRLFLKSVVDL 304
               G   L+D L++ S  +R  +K    A +L +  L+  D  R       F + ++ L
Sbjct: 189 KLANGYAGLRDALASDS--VRFQRK----ALNLLQYLLQEDDSDRSIATGLGFPRVMMHL 244

Query: 305 THLTDIDLQE 310
               D +++E
Sbjct: 249 ASSDDAEIRE 244

[Close]

BLAST of Lag0002212 vs. TAIR 10
Match: AT3G53800.1 (Fes1B )

HSP 1 Score: 78.6 bits (192), Expect = 1.3e-14
Identity = 82/288 (28.47%), Postives = 138/288 (47.92%), Query Frame = 0


Query: 65  DSMLQWAIGHSDPAKLKD-TAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAID 124
           D +L+W++ HSD A      +++ +Q     ++ H     D I ++K+ S  ++M++   
Sbjct: 9   DGLLKWSLSHSDGASSSSRISEEDRQWFVEAMQAH---TIDSISRMKVIS--QIMKMPEQ 68

Query: 125 DLKNSSLSLEDRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWIL 184
            L+   ++ +D    L EL   VE ID ANDL  +GGLV L+  L + +  +R  +A +L
Sbjct: 69  VLEAQGVTPDDLEGMLAELQEHVESIDLANDLHSIGGLVPLLSYLKNSNAKIRAKSADVL 128

Query: 185 GKASQNNPIVQKQVLELGALAKLV-RMVKSDFVEEAIKALYAISSLVRNNLFGQELFYAE 244
               QNNP  Q+ V+E      L+   +    +    KAL AISSL+RNN  G   F   
Sbjct: 129 TTVVQNNPRSQQLVMEANGFEPLLTNFIADPDIRVRTKALGAISSLIRNNQPGITAFRLA 188

Query: 245 AGETMLQDILSNSSMDIRLLKKVVFLASDLAETQLERADEVRPFFGDRLFLKSVVDLTHL 304
            G   L+D L + +  +R  +K + L   L +        VR    D  F + ++ L   
Sbjct: 189 NGYAGLRDALVSDT--VRFQRKALNLLHYLLQESNSDCKIVR----DLGFPRIMIHLASN 248

Query: 305 TDIDLQEKV--GGMALIQMKGLRMHSQALITLKNLLLLRTTKAQVLRD 349
            D +++E    G + L + + +R   +  + L+ LL  RT +  V+ D
Sbjct: 249 QDFEVREFALRGLLELAREESVRNLDRGDVNLRQLLEERTRRIIVMSD 285

[Close]

BLAST of Lag0002212 vs. TAIR 10
Match: AT5G02150.1 (Fes1C )

HSP 1 Score: 73.2 bits (178), Expect = 5.6e-13
Identity = 64/205 (31.22%), Postives = 105/205 (51.22%), Query Frame = 0


Query: 65  DSMLQWAIGHSDPAKLKDTAQDVKQLSPSELKKHQEEIKDLIEKLKMPSDAKLMQIAIDD 124
           D +L+W++ HSD    + T Q  ++      +  Q +  D++++LK     +++Q     
Sbjct: 9   DGLLKWSLSHSDGT--RPTCQLSEEDRKWFAEAMQSQTVDVVKRLK--EITQVLQTPQQV 68

Query: 125 LKNSSLSLEDRYRALQELLILVEPIDNANDLDKLGGLVVLIHELNHIDPDVRKVAAWILG 184
           L+   ++ +D    L EL   VE ID ANDL  +GGLV L+  L + + ++R  +A ++ 
Sbjct: 69  LEAHEVTPQDIEGLLDELQEHVESIDMANDLHSVGGLVPLLGYLKNSNANIRAKSADVVS 128

Query: 185 KASQNNPIVQKQVLELGALAKLVRMVKSDF-VEEAIKALYAISSLVRNNLFGQELFYAEA 244
              +NNP  Q+ V+E   L  L+    SD  +    +AL AISSL+RNN  G   F    
Sbjct: 129 TIVENNPRSQESVMEANGLESLLLRFTSDTDMHSRTQALGAISSLIRNNKPGITGFRIAN 188

Query: 245 GETMLQDILSNSSMDIRLLKKVVFL 269
           G + L+D L   S  +R  +K + L
Sbjct: 189 GYSGLKDALETDS--VRFQRKALNL 207

[Close]

BLAST of Lag0002212 vs. TAIR 10
Match: AT3G09350.2 (Fes1A )

HSP 1 Score: 72.8 bits (177), Expect = 7.3e-13
Identity = 67/212 (31.60%), Postives = 104/212 (49.06%), Query Frame = 0


Query: 99  QEEIKDLIEKLKMPSDAKLMQIAIDDLKNSSLSLEDRYRALQELLILVEPIDNANDLDKL 158
           Q +  D+++++K      +MQ     L    ++ ED    L EL   VE ID ANDL  +
Sbjct: 5   QSQTVDVVKRMK--EITLVMQTPEQVLVEHGVTPEDIQDLLDELQEHVESIDMANDLHSI 64

Query: 159 GGLVVLIHELNHIDPDVRKVAAWILGKASQNNPIVQKQVLELGALAKLVRMVKSDF-VEE 218
           GGLV L+  L +   ++R  AA ++    QNNP  Q+ V+E  AL  L+    SD  +  
Sbjct: 65  GGLVPLLSFLKNSHANIRAKAADVVSTIVQNNPRSQELVMETNALESLLSNFTSDTDIHA 124

Query: 219 AIKALYAISSLVRNNLFGQELFYAEAGETMLQDILSNSSMDIRLLKKVVFLASDLAETQL 278
             +AL AISSL+R+N  G   F    G   L+D L++ S  +R  +K    A +L +  L
Sbjct: 125 RTQALGAISSLIRHNKPGVTAFKLANGYAGLRDALASDS--VRFQRK----ALNLLQYLL 184

Query: 279 ERADEVRPFFGDRLFLKSVVDLTHLTDIDLQE 310
           +  D  R       F + ++ L    D +++E
Sbjct: 185 QEDDSDRSIATGLGFPRVMMHLASSDDAEIRE 208

The following BLAST results are available for this feature:

BLAST of Lag0002212 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025239.1	5.7e-169	75.17	FES1-1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023513988.1	3.7e-168	84.38	hsp70 nucleotide exchange factor FES1 [Cucurbita pepo subsp. pepo]	[more]
XP_022960457.1	1.4e-167	84.11	hsp70 nucleotide exchange factor FES1 [Cucurbita moschata]	[more]
XP_023004240.1	7.0e-167	83.33	hsp70 nucleotide exchange factor FES1 [Cucurbita maxima]	[more]
XP_022148873.1	3.5e-166	82.55	nucleotide exchange factor SIL1 isoform X1 [Momordica charantia]	[more]

BLAST of Lag0002212 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q32KV6	9.5e-18	36.22	Nucleotide exchange factor SIL1 OS=Bos taurus OX=9913 GN=SIL1 PE=2 SV=1	[more]
Q1E3S4	1.6e-17	34.30	Hsp70 nucleotide exchange factor FES1 OS=Coccidioides immitis (strain RS) OX=246...	[more]
A2R4I6	2.8e-17	42.98	Hsp70 nucleotide exchange factor fes1 OS=Aspergillus niger (strain CBS 513.88 / ...	[more]
Q6P6S4	1.1e-16	35.10	Nucleotide exchange factor SIL1 OS=Rattus norvegicus OX=10116 GN=Sil1 PE=2 SV=1	[more]
Q9H173	2.4e-16	33.92	Nucleotide exchange factor SIL1 OS=Homo sapiens OX=9606 GN=SIL1 PE=1 SV=1	[more]

BLAST of Lag0002212 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7G4	6.8e-168	84.11	hsp70 nucleotide exchange factor FES1 OS=Cucurbita moschata OX=3662 GN=LOC111461...	[more]
A0A6J1KU13	3.4e-167	83.33	hsp70 nucleotide exchange factor FES1 OS=Cucurbita maxima OX=3661 GN=LOC11149763...	[more]
A0A6J1D5A9	1.7e-166	82.55	nucleotide exchange factor SIL1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A0A0KA97	3.7e-166	82.90	Fes1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G089250 PE=4 S...	[more]
A0A1S3CBB7	6.2e-161	81.56	hsp70 nucleotide exchange factor FES1 OS=Cucumis melo OX=3656 GN=LOC103498900 PE...	[more]

BLAST of Lag0002212 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51980.1	2.3e-112	57.66	ARM repeat superfamily protein	[more]
AT3G09350.1	1.6e-15	30.40	Fes1A	[more]
AT3G53800.1	1.3e-14	28.47	Fes1B	[more]
AT5G02150.1	5.6e-13	31.22	Fes1C	[more]
AT3G09350.2	7.3e-13	31.60	Fes1A	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 122..142
None	No IPR available	PANTHER	PTHR19316	PROTEIN FOLDING REGULATOR	coord: 13..397
None	No IPR available	PANTHER	PTHR19316:SF29	ARM REPEAT SUPERFAMILY PROTEIN	coord: 13..397
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 95..388 e-value: 1.4E-41 score: 144.5
IPR013918	Nucleotide exchange factor Fes1	PFAM	PF08609	Fes1	coord: 64..155 e-value: 1.2E-15 score: 58.1
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 103..311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002212.1	Lag0002212.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000774	adenyl-nucleotide exchange factor activity