Lag0002205 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002205 vs. NCBI nr
Match: XP_022992031.1 (cucurbitadienol synthase [Cucurbita maxima])

HSP 1 Score: 1421.0 bits (3677), Expect = 0.0e+00
Identity = 649/727 (89.27%), Postives = 690/727 (94.91%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L+VGAES   GEK+EKW+KS+SNHLGRQVWEFC DAAA +PHQ LQ+ NAR HFHHN
Sbjct: 1   MWRLKVGAESV--GEKDEKWVKSVSNHLGRQVWEFCADAAADTPHQLLQIQNARNHFHHN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  KQ+SDL LAIQYEKEI  GAK GA+KVKEGEEV KEAVK TLERALGFYSA+QT D
Sbjct: 61  RFHRKQSSDLFLAIQYEKEIAKGAKGGAVKVKEGEEVGKEAVKSTLERALGFYSAVQTSD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           GNWASDLGGPMFL+PGLVI L+VTG LNS+LSKHHR E+CRYLYNHQNEDGGWGLHIEG 
Sbjct: 121 GNWASDLGGPMFLLPGLVIALHVTGVLNSVLSKHHRVEMCRYLYNHQNEDGGWGLHIEGT 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYVALRLLGEDADGG+GGAMTKARAWIL RGGATAI SWGKLWLS+LGVYEW
Sbjct: 181 STMFGSALNYVALRLLGEDADGGDGGAMTKARAWILERGGATAITSWGKLWLSVLGVYEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLR+ELYT
Sbjct: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPKVLSLRQELYT 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           IPYHEIDWNKS NTCAKEDL+YPHPK+QDILWGS++H+YEPLF+RWPGKR+REKALQ AM
Sbjct: 301 IPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPLFTRWPGKRLREKALQAAM 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           +HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY
Sbjct: 361 KHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDTAFSIQAI++TKLVDSY PTLRKAHDFVK+SQIQ+DCPGDPNVWFRHIHKGAW
Sbjct: 421 NGSQLWDTAFSIQAIVATKLVDSYAPTLRKAHDFVKDSQIQEDCPGDPNVWFRHIHKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           PFSTRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRLCDAVNVLLSLQNDNGGFA
Sbjct: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           SYELTRSYPWLELINP+ETFGDIVIDY YVECTAATMEAL LFKKLHPGHRTKEIDT + 
Sbjct: 541 SYELTRSYPWLELINPAETFGDIVIDYPYVECTAATMEALTLFKKLHPGHRTKEIDTAIG 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           KAANFLE +QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKAC+FLLSKEL
Sbjct: 601 KAANFLEKMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACEFLLSKEL 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           PGGGWGESYLSCQN+VYTNL+GN+PHLVNTAWVL+ALIEAGQ ERDP PLHRAARLL+NS
Sbjct: 661 PGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQGERDPAPLHRAARLLMNS 720

Query: 721 QLDNGDF 728
           QL+NGDF
Sbjct: 721 QLENGDF 725

BLAST of Lag0002205 vs. NCBI nr
Match: Q6BE24.1 (RecName: Full=Cucurbitadienol synthase [Cucurbita pepo] >BAD34645.1 cucurbitadienol synthase [Cucurbita pepo])

HSP 1 Score: 1415.6 bits (3663), Expect = 0.0e+00
Identity = 646/727 (88.86%), Postives = 689/727 (94.77%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L+VGAES   GE++EKW+KS+SNHLGRQVWEFC DAAA +PHQ LQ+ NAR HFHHN
Sbjct: 1   MWRLKVGAESV--GEEDEKWVKSVSNHLGRQVWEFCADAAADTPHQLLQIQNARNHFHHN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  KQ+SDL LAIQYEKEI  GAK GA+KVKEGEEV KEAVK TLERALGFYSA+QT D
Sbjct: 61  RFHRKQSSDLFLAIQYEKEIAKGAKGGAVKVKEGEEVGKEAVKSTLERALGFYSAVQTRD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           GNWASDLGGP+FL+PGLVI L+VTG LNS+LSKHHR E+CRYLYNHQNEDGGWGLHIEG 
Sbjct: 121 GNWASDLGGPLFLLPGLVIALHVTGVLNSVLSKHHRVEMCRYLYNHQNEDGGWGLHIEGT 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYVALRLLGEDADGG+GGAMTKARAWIL RGGATAI SWGKLWLS+LGVYEW
Sbjct: 181 STMFGSALNYVALRLLGEDADGGDGGAMTKARAWILERGGATAITSWGKLWLSVLGVYEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLR+ELYT
Sbjct: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPKVLSLRQELYT 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           IPYHEIDWNKS NTCAKEDL+YPHPK+QDILWGS++H+YEPLF+RWPGKR+REKALQ AM
Sbjct: 301 IPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPLFTRWPGKRLREKALQAAM 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           +HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY
Sbjct: 361 KHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDTAFSIQAI++TKLVDSY PTLRKAHDFVK+SQIQ+DCPGDPNVWFRHIHKGAW
Sbjct: 421 NGSQLWDTAFSIQAIVATKLVDSYAPTLRKAHDFVKDSQIQEDCPGDPNVWFRHIHKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           P STRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRLCDAVNVLLSLQNDNGGFA
Sbjct: 481 PLSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           SYELTRSYPWLELINP+ETFGDIVIDY YVECTAATMEAL LFKKLHPGHRTKEIDT + 
Sbjct: 541 SYELTRSYPWLELINPAETFGDIVIDYPYVECTAATMEALTLFKKLHPGHRTKEIDTAIG 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           KAANFLE +QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKAC+FLLSKEL
Sbjct: 601 KAANFLEKMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACEFLLSKEL 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           PGGGWGESYLSCQN+VYTNL+GN+PHLVNTAWVL+ALIEAGQ ERDP PLHRAARLL+NS
Sbjct: 661 PGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQGERDPAPLHRAARLLMNS 720

Query: 721 QLDNGDF 728
           QL+NGDF
Sbjct: 721 QLENGDF 725

BLAST of Lag0002205 vs. NCBI nr
Match: KAG7014610.1 (Cucurbitadienol synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1409.4 bits (3647), Expect = 0.0e+00
Identity = 644/727 (88.58%), Postives = 686/727 (94.36%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L+VGAES   GEK+EKW+KS+SNHLGRQVWEFC DAAAA+P Q LQ+ NAR HFH N
Sbjct: 1   MWRLKVGAESV--GEKDEKWVKSVSNHLGRQVWEFCADAAAATPQQLLQIQNARNHFHRN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  KQ+SDL LAIQYEKEI  G K GA+KVKE EEV KEAVK TLERAL FYSA+QT D
Sbjct: 61  RFHRKQSSDLFLAIQYEKEIAKGGKGGAVKVKEEEEVGKEAVKSTLERALSFYSAVQTSD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           GNWASDLGGPMFL+PGLVI LYVTG LNS+LSKHHR E+CRYLYNHQNEDGGWGLHIEG 
Sbjct: 121 GNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRVEMCRYLYNHQNEDGGWGLHIEGT 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYVALRLLGEDADGG+ GAMTKARAWIL RGGATAI SWGKLWLS+LGVYEW
Sbjct: 181 STMFGSALNYVALRLLGEDADGGDDGAMTKARAWILERGGATAITSWGKLWLSVLGVYEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLR+ELYT
Sbjct: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPKVLSLRQELYT 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           +PYHEIDWNKS NTCAKEDL+YPHPK+QDILWGS++H+YEPLF+RWPGKR+REKALQTAM
Sbjct: 301 VPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPLFTRWPGKRLREKALQTAM 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           +HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY
Sbjct: 361 KHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDTAFSIQAI++TKLVDS+ PTLRKAHDFVK+SQIQ+DCPGDPNVWFRHIHKGAW
Sbjct: 421 NGSQLWDTAFSIQAIVATKLVDSFAPTLRKAHDFVKDSQIQEDCPGDPNVWFRHIHKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           PFSTRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRLCDAVNVLLSLQNDNGGFA
Sbjct: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           SYELTRSYPWLELINP+ETFGDIVIDY YVECTAATMEAL LFKKLHPGHRTKEIDT + 
Sbjct: 541 SYELTRSYPWLELINPAETFGDIVIDYPYVECTAATMEALTLFKKLHPGHRTKEIDTAIG 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           KAANFLE +QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKAC+FLLSKEL
Sbjct: 601 KAANFLEQMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACEFLLSKEL 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           PGGGWGESYLSCQN+VYTNL+GN+PHLVNTAWVL+ALIEAGQ ERDP PLHRAARLL+NS
Sbjct: 661 PGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQGERDPAPLHRAARLLMNS 720

Query: 721 QLDNGDF 728
           QL+NGDF
Sbjct: 721 QLENGDF 725

BLAST of Lag0002205 vs. NCBI nr
Match: XP_022953219.1 (cucurbitadienol synthase [Cucurbita moschata])

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 644/728 (88.46%), Postives = 686/728 (94.23%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPD-AAAASPHQQLQLDNARKHFHH 60
           MW+L+VGAES   GEK+EKW+KS+SNHLGRQVWEFC D AAAA+P Q LQ+ NAR HFH 
Sbjct: 1   MWRLKVGAESV--GEKDEKWVKSVSNHLGRQVWEFCADAAAAATPRQLLQIQNARNHFHR 60

Query: 61  NRFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTP 120
           NRF  KQ+SDL LAIQYEKEI  G K GA+KVKE EEV KEAVK TLERAL FYSA+QT 
Sbjct: 61  NRFHRKQSSDLFLAIQYEKEIAEGGKGGAVKVKEEEEVGKEAVKSTLERALSFYSAVQTS 120

Query: 121 DGNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEG 180
           DGNWASDLGGPMFL+PGLVI LYVTG LNS+LSKHHR E+CRYLYNHQNEDGGWGLHIEG
Sbjct: 121 DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRVEMCRYLYNHQNEDGGWGLHIEG 180

Query: 181 PSTMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYE 240
            STMFGS LNYVALRLLGEDADGG+ GAMTKARAWIL RGGATAI SWGKLWLS+LGVYE
Sbjct: 181 TSTMFGSALNYVALRLLGEDADGGDDGAMTKARAWILERGGATAITSWGKLWLSVLGVYE 240

Query: 241 WSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELY 300
           WSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLR+ELY
Sbjct: 241 WSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPKVLSLRQELY 300

Query: 301 TIPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTA 360
           T+PYHEIDWNKS NTCAKEDL+YPHPK+QDILWGS++H+YEPLF+RWPGKR+REKALQTA
Sbjct: 301 TVPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPLFTRWPGKRLREKALQTA 360

Query: 361 MQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG 420
           M+HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG
Sbjct: 361 MKHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG 420

Query: 421 YNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGA 480
           YNGSQLWDTAFSIQAI++TKLVDS+ PTLRKAHDFVK+SQIQ+DCPGDPNVWFRHIHKGA
Sbjct: 421 YNGSQLWDTAFSIQAIVATKLVDSFAPTLRKAHDFVKDSQIQEDCPGDPNVWFRHIHKGA 480

Query: 481 WPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGF 540
           WPFSTRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRLCDAVNVLLSLQNDNGGF
Sbjct: 481 WPFSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRLCDAVNVLLSLQNDNGGF 540

Query: 541 ASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTL 600
           ASYELTRSYPWLELINP+ETFGDIVIDY YVECTAATMEAL LFKKLHPGHRTKEIDT +
Sbjct: 541 ASYELTRSYPWLELINPAETFGDIVIDYPYVECTAATMEALTLFKKLHPGHRTKEIDTAV 600

Query: 601 AKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKE 660
            KAANFLE +QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKAC+FLLSKE
Sbjct: 601 GKAANFLEKMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACEFLLSKE 660

Query: 661 LPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLIN 720
           LPGGGWGESYLSCQN+VYTNL+GN+PHLVNTAWVL+ALIEAGQ ERDP PLHRAARLL+N
Sbjct: 661 LPGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQGERDPAPLHRAARLLMN 720

Query: 721 SQLDNGDF 728
           SQL+NGDF
Sbjct: 721 SQLENGDF 726

BLAST of Lag0002205 vs. NCBI nr
Match: XP_023548912.1 (cucurbitadienol synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 643/729 (88.20%), Postives = 687/729 (94.24%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFC-PDAAAASPHQQLQLDNARKHFHH 60
           MW+L+VGAES   GEK+EKW+KS+SNHLGRQVWEFC  DAAA +PHQ LQ+ NAR HFH 
Sbjct: 1   MWRLKVGAESV--GEKDEKWVKSVSNHLGRQVWEFCAADAAAVTPHQLLQIQNARNHFHR 60

Query: 61  NRFSCKQASDLLLAIQYEKEIGNGAK-VGAIKVKEGEEVRKEAVKITLERALGFYSAIQT 120
           NRF  KQ+SDL LAIQYEKEI  G K   A+KVKEGEEV KEAVK TLERAL FY+A+QT
Sbjct: 61  NRFHRKQSSDLFLAIQYEKEIAKGGKGKEAVKVKEGEEVGKEAVKSTLERALSFYTAVQT 120

Query: 121 PDGNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIE 180
            DGNWASDLGGPMFL+PGLVI LYVTG LNS+LSKHHR E+CRY+YNHQNEDGGWGLHIE
Sbjct: 121 SDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRVEMCRYIYNHQNEDGGWGLHIE 180

Query: 181 GPSTMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVY 240
           G STMFGS LNYVALRLLGEDADGG+ GAMTKARAWIL RGGATAI SWGKLWLS+LGVY
Sbjct: 181 GTSTMFGSALNYVALRLLGEDADGGDDGAMTKARAWILERGGATAITSWGKLWLSVLGVY 240

Query: 241 EWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKEL 300
           EWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLR+EL
Sbjct: 241 EWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPKVLSLRQEL 300

Query: 301 YTIPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQT 360
           YT+PYHEIDWNKS NTCAKEDL+YPHPK+QDILWGS++H+YEPLF+RWPGKR+REKALQT
Sbjct: 301 YTVPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPLFTRWPGKRLREKALQT 360

Query: 361 AMQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQ 420
           AM+HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQ
Sbjct: 361 AMKHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQ 420

Query: 421 GYNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKG 480
           GYNGSQLWDTAFSIQAI++TKLVDSY PTLRKAHDFVK+SQIQ+DCPGDPNVWFRHIHKG
Sbjct: 421 GYNGSQLWDTAFSIQAIVATKLVDSYAPTLRKAHDFVKDSQIQEDCPGDPNVWFRHIHKG 480

Query: 481 AWPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGG 540
           AWPFSTRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRLCDAVNVLLSLQNDNGG
Sbjct: 481 AWPFSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRLCDAVNVLLSLQNDNGG 540

Query: 541 FASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTT 600
           FASYELTRSYPWLELINP+ETFGDIVIDYSYVECTAATMEAL LFKKLHPGHRTKEIDT 
Sbjct: 541 FASYELTRSYPWLELINPAETFGDIVIDYSYVECTAATMEALTLFKKLHPGHRTKEIDTA 600

Query: 601 LAKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSK 660
           + KAANFLE +QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKAC+FLLSK
Sbjct: 601 IGKAANFLEKMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACEFLLSK 660

Query: 661 ELPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLI 720
           ELPGGGWGESYLSCQN+VYTNL+GN+PHLVNTAWVL+ALIEAGQ ERDP PLHRAARL++
Sbjct: 661 ELPGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQGERDPAPLHRAARLVM 720

Query: 721 NSQLDNGDF 728
           NSQL+NGDF
Sbjct: 721 NSQLENGDF 727

BLAST of Lag0002205 vs. ExPASy Swiss-Prot
Match: Q6BE24 (Cucurbitadienol synthase OS=Cucurbita pepo OX=3663 GN=CPQ PE=1 SV=1)

HSP 1 Score: 1415.6 bits (3663), Expect = 0.0e+00
Identity = 646/727 (88.86%), Postives = 689/727 (94.77%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L+VGAES   GE++EKW+KS+SNHLGRQVWEFC DAAA +PHQ LQ+ NAR HFHHN
Sbjct: 1   MWRLKVGAESV--GEEDEKWVKSVSNHLGRQVWEFCADAAADTPHQLLQIQNARNHFHHN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  KQ+SDL LAIQYEKEI  GAK GA+KVKEGEEV KEAVK TLERALGFYSA+QT D
Sbjct: 61  RFHRKQSSDLFLAIQYEKEIAKGAKGGAVKVKEGEEVGKEAVKSTLERALGFYSAVQTRD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           GNWASDLGGP+FL+PGLVI L+VTG LNS+LSKHHR E+CRYLYNHQNEDGGWGLHIEG 
Sbjct: 121 GNWASDLGGPLFLLPGLVIALHVTGVLNSVLSKHHRVEMCRYLYNHQNEDGGWGLHIEGT 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYVALRLLGEDADGG+GGAMTKARAWIL RGGATAI SWGKLWLS+LGVYEW
Sbjct: 181 STMFGSALNYVALRLLGEDADGGDGGAMTKARAWILERGGATAITSWGKLWLSVLGVYEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLR+ELYT
Sbjct: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPKVLSLRQELYT 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           IPYHEIDWNKS NTCAKEDL+YPHPK+QDILWGS++H+YEPLF+RWPGKR+REKALQ AM
Sbjct: 301 IPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPLFTRWPGKRLREKALQAAM 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           +HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY
Sbjct: 361 KHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDTAFSIQAI++TKLVDSY PTLRKAHDFVK+SQIQ+DCPGDPNVWFRHIHKGAW
Sbjct: 421 NGSQLWDTAFSIQAIVATKLVDSYAPTLRKAHDFVKDSQIQEDCPGDPNVWFRHIHKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           P STRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRLCDAVNVLLSLQNDNGGFA
Sbjct: 481 PLSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           SYELTRSYPWLELINP+ETFGDIVIDY YVECTAATMEAL LFKKLHPGHRTKEIDT + 
Sbjct: 541 SYELTRSYPWLELINPAETFGDIVIDYPYVECTAATMEALTLFKKLHPGHRTKEIDTAIG 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           KAANFLE +QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKAC+FLLSKEL
Sbjct: 601 KAANFLEKMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACEFLLSKEL 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           PGGGWGESYLSCQN+VYTNL+GN+PHLVNTAWVL+ALIEAGQ ERDP PLHRAARLL+NS
Sbjct: 661 PGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQGERDPAPLHRAARLLMNS 720

Query: 721 QLDNGDF 728
           QL+NGDF
Sbjct: 721 QLENGDF 725

BLAST of Lag0002205 vs. ExPASy Swiss-Prot
Match: K7NBZ9 (Cucurbitadienol synthase OS=Siraitia grosvenorii OX=190515 GN=CBQ PE=1 SV=1)

HSP 1 Score: 1376.7 bits (3562), Expect = 0.0e+00
Identity = 630/730 (86.30%), Postives = 677/730 (92.74%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L+VGAES   GE +EKWLKSISNHLGRQVWEFCPD  A +  Q LQ+  ARK FH +
Sbjct: 1   MWRLKVGAESV--GENDEKWLKSISNHLGRQVWEFCPD--AGTQQQLLQVHKARKAFHDD 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  KQ+SDL + IQY KE+ NG K   +K+KEGEEVRKEAV+ +LERAL FYS+IQT D
Sbjct: 61  RFHRKQSSDLFITIQYGKEVENGGKTAGVKLKEGEEVRKEAVESSLERALSFYSSIQTSD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           GNWASDLGGPMFL+PGLVI LYVTG LNS+LSKHHRQE+CRY+YNHQNEDGGWGLHIEGP
Sbjct: 121 GNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRYVYNHQNEDGGWGLHIEGP 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYVALRLLGEDA+    GAM KARAWIL  GGAT I SWGKLWLS+LGVYEW
Sbjct: 181 STMFGSALNYVALRLLGEDAN---AGAMPKARAWILDHGGATGITSWGKLWLSVLGVYEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPEFWL PY LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLRKELY 
Sbjct: 241 SGNNPLPPEFWLFPYFLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIVLSLRKELYA 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           +PYHEIDWNKS NTCAKEDL+YPHPK+QDILWGSLHH+YEPLF+RWP KR+REKALQTAM
Sbjct: 301 VPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSLHHVYEPLFTRWPAKRLREKALQTAM 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           QHIHYEDENTRYICLGPVNKVLN+LCCWVEDPYSDAFKLHLQRVHDYLWVAEDGM+MQGY
Sbjct: 361 QHIHYEDENTRYICLGPVNKVLNLLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMKMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDTAFSIQAI+STKLVD+YGPTLRKAHDFVK+SQIQQDCPGDPNVW+RHIHKGAW
Sbjct: 421 NGSQLWDTAFSIQAIVSTKLVDNYGPTLRKAHDFVKSSQIQQDCPGDPNVWYRHIHKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           PFSTRDHGWLISDCTAEGLKA+LMLSKLPS+ VGE LE+NRLCDAVNVLLSLQNDNGGFA
Sbjct: 481 PFSTRDHGWLISDCTAEGLKAALMLSKLPSETVGESLERNRLCDAVNVLLSLQNDNGGFA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           SYELTRSYPWLELINP+ETFGDIVIDY YVECT+ATMEAL LFKKLHPGHRTKEIDT + 
Sbjct: 541 SYELTRSYPWLELINPAETFGDIVIDYPYVECTSATMEALTLFKKLHPGHRTKEIDTAIV 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           +AANFLEN+QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYN+CLAIRKACDFLLSKEL
Sbjct: 601 RAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNNCLAIRKACDFLLSKEL 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           PGGGWGESYLSCQN+VYTNL+GNRPHLVNTAWVL+ALIEAGQAERDP PLHRAARLLINS
Sbjct: 661 PGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMALIEAGQAERDPTPLHRAARLLINS 720

Query: 721 QLDNGDFPKR 731
           QL+NGDFP++
Sbjct: 721 QLENGDFPQQ 723

BLAST of Lag0002205 vs. ExPASy Swiss-Prot
Match: Q8W3Z4 (Cycloartenol synthase OS=Betula platyphylla OX=78630 GN=CASBPX1 PE=1 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 523/733 (71.35%), Postives = 614/733 (83.77%), Query Frame = 0

Query: 1   MWKLRVGAESA----GGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKH 60
           MWKL++GAE+A    GGG  +E WL+S++NHLGRQ+WEF P+       Q  Q+D+AR+ 
Sbjct: 1   MWKLKIGAETARGDGGGGGGSETWLRSLNNHLGRQIWEFHPELGTQEELQ--QIDDARRR 60

Query: 61  FHHNRFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAI 120
           F   RF  + +SDLL+ IQ+ KE  + A +  +K+K+ EEVR+EAV +TL RA+ FYS I
Sbjct: 61  FWERRFERRHSSDLLMRIQFAKENPSSANIPQVKIKDTEEVREEAVGMTLRRAINFYSTI 120

Query: 121 QTPDGNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLH 180
           Q  DG+W  D GGPMFLIPGLVI L +TG LN+ LSK H+ E+CRYLYNHQNEDGGWGLH
Sbjct: 121 QADDGHWPGDYGGPMFLIPGLVITLSITGTLNAFLSKEHQCEICRYLYNHQNEDGGWGLH 180

Query: 181 IEGPSTMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILG 240
           IEGPSTMFG+ LNY+ LRLLGE  DG   GA+ KAR WIL  GGATAI SWGK+WLS+LG
Sbjct: 181 IEGPSTMFGTALNYITLRLLGEPEDG--MGAVEKARKWILDHGGATAITSWGKMWLSVLG 240

Query: 241 VYEWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRK 300
           VYEWSGNNPLPPE WL PY LP HPGRMWCHCRMVYLPMSYLYGKRFVGPIT T+ SLRK
Sbjct: 241 VYEWSGNNPLPPEVWLCPYLLPCHPGRMWCHCRMVYLPMSYLYGKRFVGPITSTIQSLRK 300

Query: 301 ELYTIPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKAL 360
           ELYT+PYHEIDWNK+ N CAKEDL+YPHP +QDILW SL++ YEP+F  WP KR+REKAL
Sbjct: 301 ELYTVPYHEIDWNKARNDCAKEDLYYPHPLVQDILWASLYYAYEPIFMYWPAKRLREKAL 360

Query: 361 QTAMQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMR 420
            T MQHIHYEDENTRYIC+GPVNKVLNMLCCW EDP S+AFKLHL R+ DYLW+AEDGM+
Sbjct: 361 DTVMQHIHYEDENTRYICIGPVNKVLNMLCCWAEDPNSEAFKLHLPRILDYLWIAEDGMK 420

Query: 421 MQGYNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIH 480
           MQGYNGSQLWDT F++QAI+ST + + YG TLRKAH+++K+SQ+ +DCPGD N W+RHI 
Sbjct: 421 MQGYNGSQLWDTTFAVQAIISTNIAEEYGQTLRKAHEYIKDSQVLEDCPGDLNFWYRHIS 480

Query: 481 KGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDN 540
           KGAWPFST DHGW ISDCTAEGLKA ++LS+ PS+ VG+ ++  RL DAV+V+LSLQN +
Sbjct: 481 KGAWPFSTADHGWPISDCTAEGLKAVILLSQFPSETVGKSVDVKRLYDAVHVILSLQNTD 540

Query: 541 GGFASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEID 600
           GGFA+YELTRSY WLELINP+ETFGDIVIDY YVECT+A ++AL LFKKLHPGHR +EI+
Sbjct: 541 GGFATYELTRSYHWLELINPAETFGDIVIDYPYVECTSAAIQALTLFKKLHPGHRREEIE 600

Query: 601 TTLAKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLL 660
             +AKAA F+ENIQ  DGSWYG WGVCFTYAGWFGIKGLVAAGRTY +C +I KACD+LL
Sbjct: 601 NCIAKAAEFIENIQASDGSWYGSWGVCFTYAGWFGIKGLVAAGRTYKNCSSIHKACDYLL 660

Query: 661 SKELPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARL 720
           SKEL  GGWGESYLSCQ++VYTNLK NRPH+VNT W +LALI+AGQAERDP PLHRAAR+
Sbjct: 661 SKELASGGWGESYLSCQDKVYTNLKDNRPHIVNTGWAMLALIDAGQAERDPTPLHRAARI 720

Query: 721 LINSQLDNGDFPK 730
           LINSQ++NGDFP+
Sbjct: 721 LINSQMENGDFPQ 729

BLAST of Lag0002205 vs. ExPASy Swiss-Prot
Match: Q9SLP9 (Cycloartenol synthase OS=Luffa aegyptiaca OX=3670 GN=CAS1 PE=1 SV=1)

HSP 1 Score: 1151.7 bits (2978), Expect = 0.0e+00
Identity = 511/730 (70.00%), Postives = 617/730 (84.52%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNE-KWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHH 60
           MW+L++GA++      N   WL S++NH+GRQVW F P+       QQ+Q  +AR+ F  
Sbjct: 1   MWQLKIGADTVPADPSNAGGWLSSLNNHVGRQVWHFHPELGTPEDLQQIQ--HARQRFSD 60

Query: 61  NRFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTP 120
           +RF  K ++DLL+ +Q+ K   +   +  IKVK+ E+V +EAV  TL RA+ FYS IQ  
Sbjct: 61  HRFEKKHSADLLMRMQFAKNNSSFVNLPQIKVKDKEDVTEEAVSRTLRRAINFYSTIQGD 120

Query: 121 DGNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEG 180
           DG+W  D GGPMFLIPGLVI L +TGALN++LS  H++E+CRYLYNHQN+DGGWGLHIEG
Sbjct: 121 DGHWPGDYGGPMFLIPGLVITLSITGALNAVLSTEHQREICRYLYNHQNKDGGWGLHIEG 180

Query: 181 PSTMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYE 240
           PSTMFGS LNYV+LRLLGE+A+ G+ GA+ KAR WIL  GGA+AI SWGK+WLS+LGVYE
Sbjct: 181 PSTMFGSVLNYVSLRLLGEEAEDGQ-GAVDKARKWILDHGGASAITSWGKMWLSVLGVYE 240

Query: 241 WSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELY 300
           W+GNNPLPPE WLLPY LPFHPGRMWCHCRMVYLPM YLYGKRFVGPITP + SLRKELY
Sbjct: 241 WAGNNPLPPELWLLPYLLPFHPGRMWCHCRMVYLPMCYLYGKRFVGPITPIIRSLRKELY 300

Query: 301 TIPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTA 360
            +PYHE+DWNK+ N CAKEDL+YPHP +QDI+W SLHH+YEPLF RWP KR+REKALQ  
Sbjct: 301 LVPYHEVDWNKARNECAKEDLYYPHPLVQDIVWASLHHVYEPLFMRWPAKRLREKALQCV 360

Query: 361 MQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG 420
           MQHIHYEDENTRYIC+GPVNKVLNMLCCWVEDP+S+AFKLH+ R+ DYLW+AEDGM+MQG
Sbjct: 361 MQHIHYEDENTRYICIGPVNKVLNMLCCWVEDPHSEAFKLHIPRIFDYLWIAEDGMKMQG 420

Query: 421 YNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGA 480
           YNGSQLWDTAF++QAI+STKL + YG TLRKAH ++K+SQ+ +DCPGD   W+RHI KGA
Sbjct: 421 YNGSQLWDTAFAVQAIMSTKLAEEYGTTLRKAHKYIKDSQVLEDCPGDLQSWYRHISKGA 480

Query: 481 WPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGF 540
           WPFST DHGW ISDCTAEGLKA L+LSKLPS+IVG+ +++ ++ DAVNV+LSLQN +GGF
Sbjct: 481 WPFSTADHGWPISDCTAEGLKAVLLLSKLPSEIVGKSIDEEQIYDAVNVILSLQNTDGGF 540

Query: 541 ASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTL 600
           A+YELTRSYPWLEL+NP+ETFGDIVIDY+YVECT+A ++AL  FKKL+PGHR  EID  +
Sbjct: 541 ATYELTRSYPWLELMNPAETFGDIVIDYTYVECTSAAIQALVAFKKLYPGHRRDEIDNCV 600

Query: 601 AKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKE 660
           AKAA+F+E+IQ  DGSWYG WGVCFTY GWFGI+GLVAAGR Y++C ++RKACDFLLSKE
Sbjct: 601 AKAADFIESIQATDGSWYGSWGVCFTYGGWFGIRGLVAAGRRYDNCSSLRKACDFLLSKE 660

Query: 661 LPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLIN 720
           L  GGWGESYLS QN+VYTN+K +RPH+VNT W +L+LI+AGQ+ERDP PLHRAAR+LIN
Sbjct: 661 LASGGWGESYLSGQNKVYTNIKDDRPHIVNTGWAMLSLIDAGQSERDPTPLHRAARILIN 720

Query: 721 SQLDNGDFPK 730
           SQ+D+GDFP+
Sbjct: 721 SQMDDGDFPQ 727

BLAST of Lag0002205 vs. ExPASy Swiss-Prot
Match: Q6BE25 (Cycloartenol synthase OS=Cucurbita pepo OX=3663 GN=CPX PE=1 SV=1)

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 505/730 (69.18%), Postives = 614/730 (84.11%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNE-KWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHH 60
           MW+L++GA++      N   WL +++NH+GRQVW F P+    SP    Q+  AR+HF  
Sbjct: 1   MWQLKIGADTVPSDPSNAGGWLSTLNNHVGRQVWHFHPE--LGSPEDLQQIQQARQHFSD 60

Query: 61  NRFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTP 120
           +RF  K ++DLL+ +Q+ KE  +   +  +KVK+ E+V +EAV  TL RA+ FYS IQ  
Sbjct: 61  HRFEKKHSADLLMRMQFAKENSSFVNLPQVKVKDKEDVTEEAVTRTLRRAINFYSTIQAD 120

Query: 121 DGNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEG 180
           DG+W  D GGPMFLIPGLVI L +TGALN++LS  H++E+CRYLYNHQN+DGGWGLHIEG
Sbjct: 121 DGHWPGDYGGPMFLIPGLVITLSITGALNAVLSTEHQREICRYLYNHQNKDGGWGLHIEG 180

Query: 181 PSTMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYE 240
           PSTMFGS LNYV LRLLGE+A+ G+ GA+ KAR WIL  GGA AI SWGK+WLS+LGVYE
Sbjct: 181 PSTMFGSVLNYVTLRLLGEEAEDGQ-GAVDKARKWILDHGGAAAITSWGKMWLSVLGVYE 240

Query: 241 WSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELY 300
           W+GNNPLPPE WLLPY LP HPGRMWCHCRMVYLPM YLYGKRFVGPITP + SLRKELY
Sbjct: 241 WAGNNPLPPELWLLPYLLPCHPGRMWCHCRMVYLPMCYLYGKRFVGPITPIIRSLRKELY 300

Query: 301 TIPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTA 360
            +PYHE+DWNK+ N CAKEDL+YPHP +QDILW +LHH+YEPLF  WP KR+REKALQ+ 
Sbjct: 301 LVPYHEVDWNKARNQCAKEDLYYPHPLVQDILWATLHHVYEPLFMHWPAKRLREKALQSV 360

Query: 361 MQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG 420
           MQHIHYEDENTRYIC+GPVNKVLNMLCCW EDP+S+AFKLH+ R++DYLW+AEDGM+MQG
Sbjct: 361 MQHIHYEDENTRYICIGPVNKVLNMLCCWAEDPHSEAFKLHIPRIYDYLWIAEDGMKMQG 420

Query: 421 YNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGA 480
           YNGSQLWDTAF++QAI+ST+L + Y  TLRKAH ++K+SQ+ +DCPGD   W+RHI KGA
Sbjct: 421 YNGSQLWDTAFAVQAIISTELAEEYETTLRKAHKYIKDSQVLEDCPGDLQSWYRHISKGA 480

Query: 481 WPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGF 540
           WPFST DHGW ISDCTAEGLKA L+LSKLPS+IVG+ +++ +L +AVNV+LSLQN +GGF
Sbjct: 481 WPFSTADHGWPISDCTAEGLKAVLLLSKLPSEIVGKSIDEQQLYNAVNVILSLQNTDGGF 540

Query: 541 ASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTL 600
           A+YELTRSY WLEL+NP+ETFGDIVIDY YVEC++A ++ALA FKKL+PGHR  EID  +
Sbjct: 541 ATYELTRSYRWLELMNPAETFGDIVIDYPYVECSSAAIQALAAFKKLYPGHRRDEIDNCI 600

Query: 601 AKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKE 660
           A+AA+F+E+IQ  DGSWYG WGVCFTY GWFGI+GLVAAGR YN+C ++RKACDFLLSKE
Sbjct: 601 AEAADFIESIQATDGSWYGSWGVCFTYGGWFGIRGLVAAGRRYNNCSSLRKACDFLLSKE 660

Query: 661 LPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLIN 720
           L  GGWGESYLSCQN+VYTN+K +RPH+VNT W +L+LI+AGQ+ERDP PLHRAAR+LIN
Sbjct: 661 LAAGGWGESYLSCQNKVYTNIKDDRPHIVNTGWAMLSLIDAGQSERDPTPLHRAARVLIN 720

Query: 721 SQLDNGDFPK 730
           SQ+++GDFP+
Sbjct: 721 SQMEDGDFPQ 727

BLAST of Lag0002205 vs. ExPASy TrEMBL
Match: A0A6J1JSG0 (Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111488502 PE=3 SV=1)

HSP 1 Score: 1421.0 bits (3677), Expect = 0.0e+00
Identity = 649/727 (89.27%), Postives = 690/727 (94.91%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L+VGAES   GEK+EKW+KS+SNHLGRQVWEFC DAAA +PHQ LQ+ NAR HFHHN
Sbjct: 1   MWRLKVGAESV--GEKDEKWVKSVSNHLGRQVWEFCADAAADTPHQLLQIQNARNHFHHN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  KQ+SDL LAIQYEKEI  GAK GA+KVKEGEEV KEAVK TLERALGFYSA+QT D
Sbjct: 61  RFHRKQSSDLFLAIQYEKEIAKGAKGGAVKVKEGEEVGKEAVKSTLERALGFYSAVQTSD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           GNWASDLGGPMFL+PGLVI L+VTG LNS+LSKHHR E+CRYLYNHQNEDGGWGLHIEG 
Sbjct: 121 GNWASDLGGPMFLLPGLVIALHVTGVLNSVLSKHHRVEMCRYLYNHQNEDGGWGLHIEGT 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYVALRLLGEDADGG+GGAMTKARAWIL RGGATAI SWGKLWLS+LGVYEW
Sbjct: 181 STMFGSALNYVALRLLGEDADGGDGGAMTKARAWILERGGATAITSWGKLWLSVLGVYEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLR+ELYT
Sbjct: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPKVLSLRQELYT 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           IPYHEIDWNKS NTCAKEDL+YPHPK+QDILWGS++H+YEPLF+RWPGKR+REKALQ AM
Sbjct: 301 IPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPLFTRWPGKRLREKALQAAM 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           +HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY
Sbjct: 361 KHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDTAFSIQAI++TKLVDSY PTLRKAHDFVK+SQIQ+DCPGDPNVWFRHIHKGAW
Sbjct: 421 NGSQLWDTAFSIQAIVATKLVDSYAPTLRKAHDFVKDSQIQEDCPGDPNVWFRHIHKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           PFSTRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRLCDAVNVLLSLQNDNGGFA
Sbjct: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           SYELTRSYPWLELINP+ETFGDIVIDY YVECTAATMEAL LFKKLHPGHRTKEIDT + 
Sbjct: 541 SYELTRSYPWLELINPAETFGDIVIDYPYVECTAATMEALTLFKKLHPGHRTKEIDTAIG 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           KAANFLE +QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKAC+FLLSKEL
Sbjct: 601 KAANFLEKMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACEFLLSKEL 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           PGGGWGESYLSCQN+VYTNL+GN+PHLVNTAWVL+ALIEAGQ ERDP PLHRAARLL+NS
Sbjct: 661 PGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQGERDPAPLHRAARLLMNS 720

Query: 721 QLDNGDF 728
           QL+NGDF
Sbjct: 721 QLENGDF 725

BLAST of Lag0002205 vs. ExPASy TrEMBL
Match: A0A6J1GP10 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111455830 PE=3 SV=1)

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 644/728 (88.46%), Postives = 686/728 (94.23%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPD-AAAASPHQQLQLDNARKHFHH 60
           MW+L+VGAES   GEK+EKW+KS+SNHLGRQVWEFC D AAAA+P Q LQ+ NAR HFH 
Sbjct: 1   MWRLKVGAESV--GEKDEKWVKSVSNHLGRQVWEFCADAAAAATPRQLLQIQNARNHFHR 60

Query: 61  NRFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTP 120
           NRF  KQ+SDL LAIQYEKEI  G K GA+KVKE EEV KEAVK TLERAL FYSA+QT 
Sbjct: 61  NRFHRKQSSDLFLAIQYEKEIAEGGKGGAVKVKEEEEVGKEAVKSTLERALSFYSAVQTS 120

Query: 121 DGNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEG 180
           DGNWASDLGGPMFL+PGLVI LYVTG LNS+LSKHHR E+CRYLYNHQNEDGGWGLHIEG
Sbjct: 121 DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRVEMCRYLYNHQNEDGGWGLHIEG 180

Query: 181 PSTMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYE 240
            STMFGS LNYVALRLLGEDADGG+ GAMTKARAWIL RGGATAI SWGKLWLS+LGVYE
Sbjct: 181 TSTMFGSALNYVALRLLGEDADGGDDGAMTKARAWILERGGATAITSWGKLWLSVLGVYE 240

Query: 241 WSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELY 300
           WSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLR+ELY
Sbjct: 241 WSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPKVLSLRQELY 300

Query: 301 TIPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTA 360
           T+PYHEIDWNKS NTCAKEDL+YPHPK+QDILWGS++H+YEPLF+RWPGKR+REKALQTA
Sbjct: 301 TVPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPLFTRWPGKRLREKALQTA 360

Query: 361 MQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG 420
           M+HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG
Sbjct: 361 MKHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG 420

Query: 421 YNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGA 480
           YNGSQLWDTAFSIQAI++TKLVDS+ PTLRKAHDFVK+SQIQ+DCPGDPNVWFRHIHKGA
Sbjct: 421 YNGSQLWDTAFSIQAIVATKLVDSFAPTLRKAHDFVKDSQIQEDCPGDPNVWFRHIHKGA 480

Query: 481 WPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGF 540
           WPFSTRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRLCDAVNVLLSLQNDNGGF
Sbjct: 481 WPFSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRLCDAVNVLLSLQNDNGGF 540

Query: 541 ASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTL 600
           ASYELTRSYPWLELINP+ETFGDIVIDY YVECTAATMEAL LFKKLHPGHRTKEIDT +
Sbjct: 541 ASYELTRSYPWLELINPAETFGDIVIDYPYVECTAATMEALTLFKKLHPGHRTKEIDTAV 600

Query: 601 AKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKE 660
            KAANFLE +QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKAC+FLLSKE
Sbjct: 601 GKAANFLEKMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACEFLLSKE 660

Query: 661 LPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLIN 720
           LPGGGWGESYLSCQN+VYTNL+GN+PHLVNTAWVL+ALIEAGQ ERDP PLHRAARLL+N
Sbjct: 661 LPGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQGERDPAPLHRAARLLMN 720

Query: 721 SQLDNGDF 728
           SQL+NGDF
Sbjct: 721 SQLENGDF 726

BLAST of Lag0002205 vs. ExPASy TrEMBL
Match: A0A0D3QXV2 (Terpene cyclase/mutase family member OS=Citrullus colocynthis OX=252529 GN=CDS2 PE=2 SV=1)

HSP 1 Score: 1397.9 bits (3617), Expect = 0.0e+00
Identity = 642/729 (88.07%), Postives = 683/729 (93.69%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L+VGAES   GEK EKWLKSISNHLGRQVWEFC D   ASP+   Q+DNARKHF +N
Sbjct: 1   MWRLKVGAESV--GEKEEKWLKSISNHLGRQVWEFCADQPTASPNHLQQIDNARKHFRNN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  KQ+SDL LAIQ EKEI NG K G IKVKE E+VRKE VK T+ERAL FYSAIQT D
Sbjct: 61  RFHRKQSSDLFLAIQNEKEIANGTKGGGIKVKEEEDVRKETVKNTVERALSFYSAIQTND 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           GNWASDLGGPMFL+PGLVI LYVTG LNS+LSKHHRQE+CRYLYNHQNEDGGWGLHIEG 
Sbjct: 121 GNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRYLYNHQNEDGGWGLHIEGT 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYVALRLLGEDADGGEGGAMTKAR WIL RGGATAI SWGKLWLS+LGVYEW
Sbjct: 181 STMFGSALNYVALRLLGEDADGGEGGAMTKARGWILDRGGATAITSWGKLWLSVLGVYEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLRKELYT
Sbjct: 241 SGNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIVLSLRKELYT 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           IPYHEIDWNKS NTCAKEDL+YPHPK+QDILWGS++HLYEPLF+RWPGKR+REKALQ AM
Sbjct: 301 IPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHLYEPLFTRWPGKRLREKALQMAM 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           +HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFK HLQRV DYLW+AEDGMRMQGY
Sbjct: 361 KHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKFHLQRVPDYLWIAEDGMRMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDTAFS+QAI+STKL+DS+G TL+KAHDFVK+SQIQQD PGDPNVWFRHIHKGAW
Sbjct: 421 NGSQLWDTAFSVQAIISTKLIDSFGTTLKKAHDFVKDSQIQQDFPGDPNVWFRHIHKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           PFSTRDHGWLISDCTAEGLKASLMLSKLPS IVGEPLEK+RLCDAVNVLLSLQN+NGGFA
Sbjct: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKSRLCDAVNVLLSLQNENGGFA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           SYELTRSYPWLELINP+ETFGDIVIDY YVECT+ATMEAL LFKKLHPGHRTKEIDT +A
Sbjct: 541 SYELTRSYPWLELINPAETFGDIVIDYPYVECTSATMEALTLFKKLHPGHRTKEIDTAVA 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           KAANFLEN+QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTY++C+AIRKACDFLLSKEL
Sbjct: 601 KAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYSTCVAIRKACDFLLSKEL 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           PGGGWGESYLSCQN+VYTNL+GNRPHLVNTAWVL+ALIEAGQAERDP PLHRAARLLINS
Sbjct: 661 PGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMALIEAGQAERDPAPLHRAARLLINS 720

Query: 721 QLDNGDFPK 730
           QL+NGDFP+
Sbjct: 721 QLENGDFPQ 727

BLAST of Lag0002205 vs. ExPASy TrEMBL
Match: A0A0D3QY32 (Terpene cyclase/mutase family member OS=Citrullus colocynthis OX=252529 GN=CDS1 PE=2 SV=1)

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 640/729 (87.79%), Postives = 681/729 (93.42%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L+VGAES   GEK EKWLKSISNHLGRQVWEFC     ASP+   Q+DNAR HF +N
Sbjct: 1   MWRLKVGAESV--GEKEEKWLKSISNHLGRQVWEFCAHQPTASPNHLQQIDNARNHFRNN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  KQ+SDL LAIQ EKEI N  K G IKVKE E+VRKE VK T+ERAL FYSAIQT D
Sbjct: 61  RFHRKQSSDLFLAIQNEKEIANVTKGGGIKVKEEEDVRKETVKNTVERALSFYSAIQTND 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           GNWASDLGGPMFL+PGLVI LYVTG LNS+LSKHHRQE+CRYLYNHQNEDGGWGLHIEG 
Sbjct: 121 GNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRYLYNHQNEDGGWGLHIEGT 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYVALRLLGEDADGGEGGAMTKAR+WIL RGGATAI SWGKLWLS+LGVYEW
Sbjct: 181 STMFGSALNYVALRLLGEDADGGEGGAMTKARSWILDRGGATAITSWGKLWLSVLGVYEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLRKELYT
Sbjct: 241 SGNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIVLSLRKELYT 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           IPYHEIDWN+S NTCAKEDL+YPHPK+QDILWGS++HLYEPLF+RWPGKR+REKALQ AM
Sbjct: 301 IPYHEIDWNRSRNTCAKEDLYYPHPKMQDILWGSIYHLYEPLFTRWPGKRLREKALQMAM 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           +HIHYEDEN+RYICLGPVNKVLNMLCCWVEDPYSDAFK HLQRV DYLWVAEDGMRMQGY
Sbjct: 361 KHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKFHLQRVPDYLWVAEDGMRMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDTAFS+QAI+STKL+DS+G TL+KAHDFVK+SQIQQDCPGDPNVWFRHIHKGAW
Sbjct: 421 NGSQLWDTAFSVQAIISTKLIDSFGTTLKKAHDFVKDSQIQQDCPGDPNVWFRHIHKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           PFSTRDHGWLISDCTAEGLKASLMLSKLPS IVGEPLEK+RLCDAVNVLLSLQN+NGGFA
Sbjct: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKSRLCDAVNVLLSLQNENGGFA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           SYELTRSYPWLELINP+ETFGDIVIDY YVECT+ATMEAL LFKKLHPGHRTKEID  +A
Sbjct: 541 SYELTRSYPWLELINPAETFGDIVIDYPYVECTSATMEALTLFKKLHPGHRTKEIDIAVA 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           +AANFLEN+QR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSC+AIRKACDFLLSKEL
Sbjct: 601 RAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCVAIRKACDFLLSKEL 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           PGGGWGESYLSCQN+VYTNL+GNRPHLVNTAWVL+ALIEAGQAERDP PLHRAARLLINS
Sbjct: 661 PGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMALIEAGQAERDPAPLHRAARLLINS 720

Query: 721 QLDNGDFPK 730
           QL+NGDFP+
Sbjct: 721 QLENGDFPQ 727

BLAST of Lag0002205 vs. ExPASy TrEMBL
Match: A0A5A4WLU4 (Terpene cyclase/mutase family member OS=Momordica charantia var. pavel OX=1300971 GN=CBS PE=2 SV=1)

HSP 1 Score: 1388.6 bits (3593), Expect = 0.0e+00
Identity = 636/729 (87.24%), Postives = 682/729 (93.55%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L+VGAES   GE +EKW+KSISNHLGRQVWEFCPD  A +P Q LQ++ ARK F  N
Sbjct: 1   MWRLKVGAESV--GENDEKWVKSISNHLGRQVWEFCPD--AGTPQQLLQIEKARKAFQDN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  KQ SDLL++IQ EK   NGA+V   K+KEGEEVRKEAVK TLERAL FYS+IQT D
Sbjct: 61  RFHRKQTSDLLVSIQCEKGTTNGARVPGTKLKEGEEVRKEAVKSTLERALSFYSSIQTSD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           GNWASDLGGPMFL+PGLVI L VTGALNS+LSKHHRQE+CRYLYNHQNEDGGWGLHIE P
Sbjct: 121 GNWASDLGGPMFLLPGLVIALCVTGALNSVLSKHHRQEMCRYLYNHQNEDGGWGLHIESP 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYVALRLLGEDADGGEG AMTKARAWIL  GGATAI SWGKLWLS+LGVYEW
Sbjct: 181 STMFGSALNYVALRLLGEDADGGEGRAMTKARAWILGHGGATAITSWGKLWLSVLGVYEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP VLSLRKELYT
Sbjct: 241 SGNNPLPPEFWLLPYFLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPVVLSLRKELYT 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           +PYHEIDWNKS NTCAKEDL+YPH K+QDILWGS+HH+YEPLF+ WP KR+REKAL+TAM
Sbjct: 301 VPYHEIDWNKSRNTCAKEDLYYPHSKMQDILWGSIHHMYEPLFTHWPAKRLREKALKTAM 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYS+AFKLHLQRVHDYLWVAEDGM+MQGY
Sbjct: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSEAFKLHLQRVHDYLWVAEDGMKMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDTAFS+QAI+STKLVD+YGPTLRKAHD+VKNSQIQQDCPG+PNVWFRHIHKGAW
Sbjct: 421 NGSQLWDTAFSVQAIISTKLVDNYGPTLRKAHDYVKNSQIQQDCPGEPNVWFRHIHKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           PFSTRDHGWLISDCTAEGLKASLMLSKLPS+ VGEPLE+NRLCDAVNVLLSLQNDNGGFA
Sbjct: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSETVGEPLERNRLCDAVNVLLSLQNDNGGFA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           SYELTRSYPWLELINP+ETFGDIVIDY YVECT+ATMEALALFKKLHPGHRTKEIDT +A
Sbjct: 541 SYELTRSYPWLELINPAETFGDIVIDYPYVECTSATMEALALFKKLHPGHRTKEIDTAIA 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           +AA+FLEN+QR DGSWYGCWGVCFTYAGWFGIKGLVAAGR Y++CLAIRKACDFLLSKEL
Sbjct: 601 RAADFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRAYSNCLAIRKACDFLLSKEL 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           PGGGWGESYLSCQN+VYTNL+GNRPHLVNTAWVL+ALIEAGQ ERDP PLHRAARLLINS
Sbjct: 661 PGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMALIEAGQGERDPAPLHRAARLLINS 720

Query: 721 QLDNGDFPK 730
           QL+NGDFP+
Sbjct: 721 QLENGDFPQ 725

BLAST of Lag0002205 vs. TAIR 10
Match: AT2G07050.1 (cycloartenol synthase 1 )

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 488/730 (66.85%), Postives = 591/730 (80.96%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MWKL++        E    WL++ +NH+GRQ WEF P+    +P     ++ ARK F  N
Sbjct: 1   MWKLKI-------AEGGSPWLRTTNNHVGRQFWEFDPN--LGTPEDLAAVEEARKSFSDN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  K ++DLL+ +Q+ +E      +  +K+++ ++V +E V+ TL+R L FYS IQ  D
Sbjct: 61  RFVQKHSADLLMRLQFSRENLISPVLPQVKIEDTDDVTEEMVETTLKRGLDFYSTIQAHD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           G+W  D GGPMFL+PGL+I L +TGALN++LS+ H+QE+ RYLYNHQNEDGGWGLHIEGP
Sbjct: 121 GHWPGDYGGPMFLLPGLIITLSITGALNTVLSEQHKQEMRRYLYNHQNEDGGWGLHIEGP 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMFGS LNYV LRLLGE  + G+G  M K R WIL  GGAT I SWGK+WLS+LG +EW
Sbjct: 181 STMFGSVLNYVTLRLLGEGPNDGDGD-MEKGRDWILNHGGATNITSWGKMWLSVLGAFEW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SGNNPLPPE WLLPY LP HPGRMWCHCRMVYLPMSYLYGKRFVGPIT TVLSLRKEL+T
Sbjct: 241 SGNNPLPPEIWLLPYFLPIHPGRMWCHCRMVYLPMSYLYGKRFVGPITSTVLSLRKELFT 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQTAM 360
           +PYHE++WN++ N CAKEDL+YPHP +QDILW SLH + EP+  RWPG  +REKA++TA+
Sbjct: 301 VPYHEVNWNEARNLCAKEDLYYPHPLVQDILWASLHKIVEPVLMRWPGANLREKAIRTAI 360

Query: 361 QHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQGY 420
           +HIHYEDENTRYIC+GPVNKVLNMLCCWVEDP S+AFKLHL R+HD+LW+AEDGM+MQGY
Sbjct: 361 EHIHYEDENTRYICIGPVNKVLNMLCCWVEDPNSEAFKLHLPRIHDFLWLAEDGMKMQGY 420

Query: 421 NGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGAW 480
           NGSQLWDT F+IQAIL+T LV+ YGP L KAH FVKNSQ+ +DCPGD N W+RHI KGAW
Sbjct: 421 NGSQLWDTGFAIQAILATNLVEEYGPVLEKAHSFVKNSQVLEDCPGDLNYWYRHISKGAW 480

Query: 481 PFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGFA 540
           PFST DHGW ISDCTAEGLKA+L+LSK+P  IVGEP++  RL +AVNV++SLQN +GG A
Sbjct: 481 PFSTADHGWPISDCTAEGLKAALLLSKVPKAIVGEPIDAKRLYEAVNVIISLQNADGGLA 540

Query: 541 SYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTLA 600
           +YELTRSYPWLELINP+ETFGDIVIDY YVECT+A ++AL  F+KL+PGHR KE+D  + 
Sbjct: 541 TYELTRSYPWLELINPAETFGDIVIDYPYVECTSAAIQALISFRKLYPGHRKKEVDECIE 600

Query: 601 KAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKEL 660
           KA  F+E+IQ  DGSWYG W VCFTY  WFG+KGLVA G+T  +   + KAC+FLLSK+ 
Sbjct: 601 KAVKFIESIQAADGSWYGSWAVCFTYGTWFGVKGLVAVGKTLKNSPHVAKACEFLLSKQQ 660

Query: 661 PGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLINS 720
           P GGWGESYLSCQ++VY+NL GNR H+VNTAW +LALI AGQAE D KPLHRAAR LIN+
Sbjct: 661 PSGGWGESYLSCQDKVYSNLDGNRSHVVNTAWAMLALIGAGQAEVDRKPLHRAARYLINA 720

Query: 721 QLDNGDFPKR 731
           Q++NGDFP++
Sbjct: 721 QMENGDFPQQ 720

BLAST of Lag0002205 vs. TAIR 10
Match: AT3G45130.1 (lanosterol synthase 1 )

HSP 1 Score: 1008.8 bits (2607), Expect = 2.2e-294
Identity = 464/733 (63.30%), Postives = 568/733 (77.49%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L++   S G  E       S++ H+GRQ WE+  D    +  ++  +++ R +F  N
Sbjct: 1   MWRLKL---SEGDEE-------SVNQHVGRQFWEY--DNQFGTSEERHHINHLRSNFTLN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNG-AKVGAIKVKEGEE--VRKEAVKITLERALGFYSAIQ 120
           RFS K +SDLL   Q  KE G G  ++  +KVKEGEE  + +E V +TL R+L FYS +Q
Sbjct: 61  RFSSKHSSDLLYRFQCWKEKGKGMERLPQVKVKEGEERLINEEVVNVTLRRSLRFYSILQ 120

Query: 121 TPDGNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHI 180
           + DG W  D GGP+FL+P LVIGLYVT  L+  L+  H+ E+ RYLYNHQN+DGGWGLH+
Sbjct: 121 SQDGFWPGDYGGPLFLLPALVIGLYVTEVLDGTLTAQHQIEIRRYLYNHQNKDGGWGLHV 180

Query: 181 EGPSTMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGV 240
           EG STMF + L+YVALRL+GE+ DGG+ GAM  AR+WI   GGAT IPSWGK WLS+LG 
Sbjct: 181 EGNSTMFCTVLSYVALRLMGEELDGGD-GAMESARSWIHHHGGATFIPSWGKFWLSVLGA 240

Query: 241 YEWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKE 300
           YEWSGNNPLPPE WLLPYSLPFHPGRMWCHCRMVYLPMSYLYG+RFV     T+LSLR+E
Sbjct: 241 YEWSGNNPLPPELWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGRRFVCRTNGTILSLRRE 300

Query: 301 LYTIPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKRIREKALQ 360
           LYTIPYH IDW+ + N CAKEDL+YPHPKIQD+LW  L+   EPL  RWP   +R  ALQ
Sbjct: 301 LYTIPYHHIDWDTARNQCAKEDLYYPHPKIQDVLWSCLNKFGEPLLERWPLNNLRNHALQ 360

Query: 361 TAMQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRM 420
           T MQHIHYED+N+ YIC+GPVNKVLNMLCCWVE   S+AFK HL R+ DYLWVAEDGM+M
Sbjct: 361 TVMQHIHYEDQNSHYICIGPVNKVLNMLCCWVESSNSEAFKSHLSRIKDYLWVAEDGMKM 420

Query: 421 QGYNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHK 480
           QGYNGSQLWD   ++QAIL+T LVD YG  L+KAH+++KN+QI++D  GDP +W+RH  K
Sbjct: 421 QGYNGSQLWDVTLAVQAILATNLVDDYGLMLKKAHNYIKNTQIRKDTSGDPGLWYRHPCK 480

Query: 481 GAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNG 540
           G W FST D+ W +SDCTAE LKA+L+LS++P ++VGEP+ +  L DAVN +LSLQN NG
Sbjct: 481 GGWGFSTGDNPWPVSDCTAEALKAALLLSQMPVNLVGEPMPEEHLVDAVNFILSLQNKNG 540

Query: 541 GFASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDT 600
           GFASYELTRSYP LE+INPSETFGDI+IDY YVECT+A ++ L LF  L+  ++ KEI  
Sbjct: 541 GFASYELTRSYPELEVINPSETFGDIIIDYQYVECTSAAIQGLVLFTTLNSSYKRKEIVG 600

Query: 601 TLAKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLS 660
           ++ KA  F+E  Q PDGSWYG WGVCFTYA WFGIKG++A+G+TY S L IRKAC FLLS
Sbjct: 601 SINKAVEFIEKTQLPDGSWYGSWGVCFTYATWFGIKGMLASGKTYESSLCIRKACGFLLS 660

Query: 661 KELPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLL 720
           K+L  GGWGESYLSCQN+VYTNL GN+ H+VNT+W LLALIEAGQA RDP PLHR A+ L
Sbjct: 661 KQLCCGGWGESYLSCQNKVYTNLPGNKSHIVNTSWALLALIEAGQASRDPMPLHRGAKSL 720

Query: 721 INSQLDNGDFPKR 731
           INSQ+++GD+P++
Sbjct: 721 INSQMEDGDYPQQ 720

BLAST of Lag0002205 vs. TAIR 10
Match: AT1G78955.1 (camelliol C synthase 1 )

HSP 1 Score: 880.9 bits (2275), Expect = 6.8e-256
Identity = 396/731 (54.17%), Postives = 529/731 (72.37%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MWKL++       G K E +L S +N LGRQ WEF PDA      +   ++ AR+ F+ +
Sbjct: 1   MWKLKI-----ANGNKEEPYLFSTNNFLGRQTWEFDPDAGTV--EELAAVEEARRKFYDD 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  K +SDL+  +Q+ KE      +   KV++   +  E     L + + F SA+Q  D
Sbjct: 61  RFRVKASSDLIWRMQFLKEKKFEQVIPPAKVEDANNITSEIATNALRKGVNFLSALQASD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           G+W ++  GP+F +P LV  LYVTG L+ I ++ HR+E+ RY+Y HQNEDGGWGLHIEG 
Sbjct: 121 GHWPAENAGPLFFLPPLVFCLYVTGHLHEIFTQDHRREVLRYIYCHQNEDGGWGLHIEGN 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMF + LNY+ +R+LGE  +GG G A  +AR WIL  GGAT IPSWGK WLSILGV++W
Sbjct: 181 STMFCTTLNYICMRILGEGPNGGPGNACKRARDWILDHGGATYIPSWGKTWLSILGVFDW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SG+NP+PPEFW+LP  LP HP +MWC+CR+VY+PMSYLYGKRFVGPI+P +L LR+E+Y 
Sbjct: 241 SGSNPMPPEFWILPSFLPIHPAKMWCYCRLVYMPMSYLYGKRFVGPISPLILQLREEIYL 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWP-GKRIREKALQTA 360
            PY +I+WN++ + CAKED + PHP+IQD++W  L+   EP  + WP  K +REKAL  A
Sbjct: 301 QPYAKINWNRARHLCAKEDAYCPHPQIQDVIWNCLYIFTEPFLACWPFNKLLREKALGVA 360

Query: 361 MQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG 420
           M+HIHYEDEN+RYI +G V K L ML CWVEDP    FK HL R+ DYLW+AEDGM+MQ 
Sbjct: 361 MKHIHYEDENSRYITIGCVEKALCMLACWVEDPNGIHFKKHLLRISDYLWIAEDGMKMQS 420

Query: 421 YNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGA 480
           + GSQLWD+ F++QA++++ LV+     LR+ +DF+KNSQ++++  GD    +RHI KG+
Sbjct: 421 F-GSQLWDSGFALQALVASNLVNEIPDVLRRGYDFLKNSQVRENPSGDFTNMYRHISKGS 480

Query: 481 WPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGF 540
           W FS RDHGW  SDCTAE  K  L+LS +P DIVG  ++  +L +AV +LLSLQ+ NGG 
Sbjct: 481 WTFSDRDHGWQASDCTAESFKCCLLLSMIPPDIVGPKMDPEQLYEAVTILLSLQSKNGGV 540

Query: 541 ASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTL 600
            ++E  R   WLEL+NP+E F DIV+++ Y ECT++ ++AL LFK+L+P HRT+EI+T++
Sbjct: 541 TAWEPARGQEWLELLNPTEVFADIVVEHEYNECTSSAIQALILFKQLYPNHRTEEINTSI 600

Query: 601 AKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKE 660
            KA  ++E+IQ  DGSWYG WGVCFTY+ WFG+ GL AAG+TYN+CLA+RK   FLL+ +
Sbjct: 601 KKAVQYIESIQMLDGSWYGSWGVCFTYSTWFGLGGLAAAGKTYNNCLAMRKGVHFLLTTQ 660

Query: 661 LPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLIN 720
              GGWGESYLSC  + Y   +G R +LV T+W ++ L+ AGQAERDP PLHRAA+LLIN
Sbjct: 661 KDNGGWGESYLSCPKKRYIPSEGERSNLVQTSWAMMGLLHAGQAERDPSPLHRAAKLLIN 720

Query: 721 SQLDNGDFPKR 731
           SQL+NGDFP++
Sbjct: 721 SQLENGDFPQQ 723

BLAST of Lag0002205 vs. TAIR 10
Match: AT1G78950.1 (Terpenoid cyclases family protein )

HSP 1 Score: 877.9 bits (2267), Expect = 5.8e-255
Identity = 399/731 (54.58%), Postives = 521/731 (71.27%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MW+L++G      G  ++ +L + +N  GRQ WEF PD    SP ++  +  AR+ F+ N
Sbjct: 1   MWRLKIGE-----GNGDDPYLFTTNNFAGRQTWEFDPD--GGSPEERHSVVEARRIFYDN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           RF  K +SDLL  +Q+ +E     ++  +KV++ E+V  E     L R + F+SA+Q  D
Sbjct: 61  RFHVKASSDLLWRMQFLREKKFEQRIAPVKVEDSEKVTFETATSALRRGIHFFSALQASD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           G+W ++  GP+F +P LV  LY+TG L+ + +  HR+E+ RY+Y HQ EDGGWGLHIEG 
Sbjct: 121 GHWPAENAGPLFFLPPLVFCLYITGHLDEVFTSEHRKEILRYIYCHQKEDGGWGLHIEGH 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           STMF + LNY+ +R+LGE  DGG   A  +AR WIL+ GG T IPSWGK WLSILGV++W
Sbjct: 181 STMFCTTLNYICMRILGESPDGGHDNACGRAREWILSHGGVTYIPSWGKTWLSILGVFDW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SG+NP+PPEFW+LP   P HP +MW +CRMVYLPMSYLYGKRFVGPIT  +L LRKELY 
Sbjct: 241 SGSNPMPPEFWILPSFFPVHPAKMWSYCRMVYLPMSYLYGKRFVGPITSLILQLRKELYL 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWP-GKRIREKALQTA 360
            PY EI+W K  + CAKED +YP P +Q+++W SL+   EP  +RWP  K +REKALQ A
Sbjct: 301 QPYEEINWMKVRHLCAKEDTYYPRPLVQELVWDSLYIFAEPFLARWPFNKLLREKALQLA 360

Query: 361 MQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG 420
           M+HIHYEDEN+RYI +G V KVL ML CWVEDP  D FK HL R+ DYLW+AEDGM+MQ 
Sbjct: 361 MKHIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKKHLSRISDYLWMAEDGMKMQS 420

Query: 421 YNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGA 480
           + GSQLWDT F++QA+L++ L       LR+ H+F+KNSQ+ ++  GD    +RHI KGA
Sbjct: 421 F-GSQLWDTGFAMQALLASNLSSEISDVLRRGHEFIKNSQVGENPSGDYKSMYRHISKGA 480

Query: 481 WPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGF 540
           W FS RDHGW +SDCTA GLK  L+ S L  DIVG   +  RL D+VN+LLSLQ+ NGG 
Sbjct: 481 WTFSDRDHGWQVSDCTAHGLKCCLLFSMLAPDIVGPKQDPERLHDSVNILLSLQSKNGGM 540

Query: 541 ASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTL 600
            ++E   +  WLEL+NP+E F DIVI++ Y ECT++ ++AL+LFK+L+P HRT EI   +
Sbjct: 541 TAWEPAGAPKWLELLNPTEMFSDIVIEHEYSECTSSAIQALSLFKQLYPDHRTTEITAFI 600

Query: 601 AKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKE 660
            KAA +LEN+Q  DGSWYG WG+CFTY  WF + GL AAG+T+N C AIRK   FLL+ +
Sbjct: 601 KKAAEYLENMQTRDGSWYGNWGICFTYGTWFALAGLAAAGKTFNDCEAIRKGVQFLLAAQ 660

Query: 661 LPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLIN 720
              GGWGESYLSC  ++Y    G   ++V TAW L+ LI +GQAERDP PLHRAA+L+IN
Sbjct: 661 KDNGGWGESYLSCSKKIYIAQVGEISNVVQTAWALMGLIHSGQAERDPIPLHRAAKLIIN 720

Query: 721 SQLDNGDFPKR 731
           SQL++GDFP++
Sbjct: 721 SQLESGDFPQQ 723

BLAST of Lag0002205 vs. TAIR 10
Match: AT1G78960.1 (lupeol synthase 2 )

HSP 1 Score: 863.2 bits (2229), Expect = 1.5e-250
Identity = 389/731 (53.21%), Postives = 524/731 (71.68%), Query Frame = 0

Query: 1   MWKLRVGAESAGGGEKNEKWLKSISNHLGRQVWEFCPDAAAASPHQQLQLDNARKHFHHN 60
           MWKL++G    G GE  + +L S +N +GRQ WEF  D  A +P ++  +++AR+++  N
Sbjct: 1   MWKLKIG---EGNGE--DPYLFSSNNFVGRQTWEF--DPKAGTPEERAAVEDARRNYLDN 60

Query: 61  RFSCKQASDLLLAIQYEKEIGNGAKVGAIKVKEGEEVRKEAVKITLERALGFYSAIQTPD 120
           R   K  SDLL  +Q+ KE      +  +K+ +GE +  +     L RA+ FYSA+Q+ D
Sbjct: 61  RPRVKGCSDLLWRMQFLKEAKFEQVIPPVKIDDGEGITYKNATDALRRAVSFYSALQSSD 120

Query: 121 GNWASDLGGPMFLIPGLVIGLYVTGALNSILSKHHRQELCRYLYNHQNEDGGWGLHIEGP 180
           G+W +++ G +F +P LV   Y+TG L  I    HR+E+ R++Y HQNEDGGWGLHIEG 
Sbjct: 121 GHWPAEITGTLFFLPPLVFCFYITGHLEKIFDAEHRKEMLRHIYCHQNEDGGWGLHIEGK 180

Query: 181 STMFGSGLNYVALRLLGEDADGGEGGAMTKARAWILARGGATAIPSWGKLWLSILGVYEW 240
           S MF + LNY+ LR+LGE  +GG   A  +AR WIL  GG T IPSWGK+WLSILG+Y+W
Sbjct: 181 SVMFCTVLNYICLRMLGEGPNGGRNNACKRARQWILDHGGVTYIPSWGKIWLSILGIYDW 240

Query: 241 SGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELYT 300
           SG NP+PPE WLLP   P H G+  C+ RMVY+PMSYLYGKRFVGP+TP ++ LRKEL+ 
Sbjct: 241 SGTNPMPPEIWLLPSFFPIHLGKTLCYTRMVYMPMSYLYGKRFVGPLTPLIMLLRKELHL 300

Query: 301 IPYHEIDWNKSCNTCAKEDLFYPHPKIQDILWGSLHHLYEPLFSRWPGKR-IREKALQTA 360
            PY EI+WNK+   CAKED+ YPHP +QD+LW +LH+  EP+ + WP K+ +REKAL+ A
Sbjct: 301 QPYEEINWNKARRLCAKEDMIYPHPLVQDLLWDTLHNFVEPILTNWPLKKLVREKALRVA 360

Query: 361 MQHIHYEDENTRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQRVHDYLWVAEDGMRMQG 420
           M+HIHYEDEN+ YI +G V KVL ML CW+E+P  D FK HL R+ D++WVAEDG++MQ 
Sbjct: 361 MEHIHYEDENSHYITIGCVEKVLCMLACWIENPNGDHFKKHLARIPDFMWVAEDGLKMQS 420

Query: 421 YNGSQLWDTAFSIQAILSTKLVDSYGPTLRKAHDFVKNSQIQQDCPGDPNVWFRHIHKGA 480
           + GSQLWDT F+IQA+L+  L D     LRK H F+K SQ++++  GD    +RHI KGA
Sbjct: 421 F-GSQLWDTVFAIQALLACDLSDETDDVLRKGHSFIKKSQVRENPSGDFKSMYRHISKGA 480

Query: 481 WPFSTRDHGWLISDCTAEGLKASLMLSKLPSDIVGEPLEKNRLCDAVNVLLSLQNDNGGF 540
           W  S RDHGW +SDCTAE LK  ++LS +P+++VG+ ++  +L D+VN+LLSLQ + GG 
Sbjct: 481 WTLSDRDHGWQVSDCTAEALKCCMLLSMMPAEVVGQKIDPEQLYDSVNLLLSLQGEKGGL 540

Query: 541 ASYELTRSYPWLELINPSETFGDIVIDYSYVECTAATMEALALFKKLHPGHRTKEIDTTL 600
            ++E  R+  WLEL+NP++ F  ++ +  YVECT+A ++AL LFK+L+P HRTKEI  ++
Sbjct: 541 TAWEPVRAQEWLELLNPTDFFTCVMAEREYVECTSAVIQALVLFKQLYPDHRTKEIIKSI 600

Query: 601 AKAANFLENIQRPDGSWYGCWGVCFTYAGWFGIKGLVAAGRTYNSCLAIRKACDFLLSKE 660
            K   F+E+ Q PDGSW+G WG+CF YA WF + GL AAG+TY SCLA+RK  DFLL+ +
Sbjct: 601 EKGVQFIESKQTPDGSWHGNWGICFIYATWFALSGLAAAGKTYKSCLAVRKGVDFLLAIQ 660

Query: 661 LPGGGWGESYLSCQNRVYTNLKGNRPHLVNTAWVLLALIEAGQAERDPKPLHRAARLLIN 720
              GGWGES+LSC  + Y  L+GNR +LV TAW ++ LI AGQAERDP PLHRAA+L+I 
Sbjct: 661 EEDGGWGESHLSCPEQRYIPLEGNRSNLVQTAWAMMGLIHAGQAERDPTPLHRAAKLIIT 720

Query: 721 SQLDNGDFPKR 731
           SQL+NGDFP++
Sbjct: 721 SQLENGDFPQQ 723