Lag0002204 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002204 vs. NCBI nr
Match: XP_004140662.1 (cytochrome P450 81Q32 [Cucumis sativus] >AIY67847.1 cytochrome P450 [Cucumis sativus] >KGN46390.1 hypothetical protein Csa_005013 [Cucumis sativus])

HSP 1 Score: 847.8 bits (2189), Expect = 4.8e-242
Identity = 425/510 (83.33%), Postives = 461/510 (90.39%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTKNLPPSPLALPVIGHLHLLKHPIHRTLQ 65
           ME    T+   LI  FL + +IF +Q+   T KNLPPSPLALP++GHLHLLKHPIHRTL 
Sbjct: 1   MEMEAITYLPFLIT-FLALVSIFFIQK---TKKNLPPSPLALPILGHLHLLKHPIHRTLH 60

Query: 66  TLSQKHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGA 125
            LSQK+GPIFTLRLGSRLVVVVSS SA+EECFTTNDIVFANRPEFVSGKYLTYGNSTLGA
Sbjct: 61  NLSQKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGA 120

Query: 126 APYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFS 185
           APYGDHWRNLRRLSATE+LS+IRLN SA IR+EEIGIL++KL+RV G +FG+VKLKSLFS
Sbjct: 121 APYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFS 180

Query: 186 ELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQYTK 245
           ELTFNIIMRM+AGKRYYGEEV+ELEEAKKFREIME+AFQLGSYPGDFLPFLKWVD QYTK
Sbjct: 181 ELTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTK 240

Query: 246 RVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTI 305
           RVESLG+DTD FLQNLVDEHRR+E EG+K NTMISHLLCLQKTQPDYYTDEIIKGLIVTI
Sbjct: 241 RVESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTI 300

Query: 306 LSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISE 365
           LSAATETSSVTIEWAMSNLLNHPEAL KVKDELDK IGQ+R ++E+DI NL YLQNVISE
Sbjct: 301 LSAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISE 360

Query: 366 TLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQN 425
           TLRLYP APLLAPHLSS SCSLGGYHIP DT+LMVNAWA+QRDPKVW+DSTSFKPERF++
Sbjct: 361 TLRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFES 420

Query: 426 ------GGSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDM 485
                 G +  NN Y FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKR+  KEIDM
Sbjct: 421 DHQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDM 480

Query: 486 TEGQGISMPKVEPLEALCKARPIMKKLASD 510
           TEGQGISMPKVEPLEALC+ARPI+KKLA D
Sbjct: 481 TEGQGISMPKVEPLEALCRARPIIKKLALD 506

BLAST of Lag0002204 vs. NCBI nr
Match: KAA0039876.1 (cytochrome P450 81E8-like [Cucumis melo var. makuwa] >TYK24625.1 cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 838.2 bits (2164), Expect = 3.8e-239
Identity = 424/510 (83.14%), Postives = 459/510 (90.00%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTK--NLPPSPLALPVIGHLHLLKHPIHRT 65
           MET    +F  LI   L + +IF +++ K  TK  NLPPSPL LP++GHLHLLKHPIHRT
Sbjct: 1   MET--NAYFPFLIT-LLALISIFFIRKSKTKTKRNNLPPSPLPLPILGHLHLLKHPIHRT 60

Query: 66  LQTLSQKHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTL 125
           L TLSQK+GPIFTLRLGSRLVVVVSSTSA EECFTTNDIVFANRPEFVSGKYLTYGNSTL
Sbjct: 61  LHTLSQKYGPIFTLRLGSRLVVVVSSTSAAEECFTTNDIVFANRPEFVSGKYLTYGNSTL 120

Query: 126 GAAPYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSL 185
           GAAPYGDHWRNLRRLSATEVLS+IRLN SA IR+EEI IL++KL+RVS  DFG+VKLKSL
Sbjct: 121 GAAPYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRVSSTDFGKVKLKSL 180

Query: 186 FSELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQY 245
           FSELTFN+IMRMVAGKRYYGEEV+ELEEAKKFREIME+AFQLGSYPGDFLPFLKWVD QY
Sbjct: 181 FSELTFNMIMRMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQY 240

Query: 246 TKRVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIV 305
           TKRVESLG+DTD FLQNLVDEHR +E EG+K NTM+SHLL LQKTQPDYYTDEIIKGLIV
Sbjct: 241 TKRVESLGKDTDWFLQNLVDEHRSNEEEGEKGNTMVSHLLRLQKTQPDYYTDEIIKGLIV 300

Query: 306 TILSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVI 365
           TILSAATETSSVTIEWAMSNLLNHPEAL KVK+ELD  IGQ+R ++E+DI NL YLQNVI
Sbjct: 301 TILSAATETSSVTIEWAMSNLLNHPEALKKVKEELDTQIGQDRILDEEDISNLPYLQNVI 360

Query: 366 SETLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERF 425
           SETLRLYP APLLAPHLSS SCSLGGYHIP DTILMVNAWA+QRDPKVW+DSTSFKPERF
Sbjct: 361 SETLRLYPPAPLLAPHLSSSSCSLGGYHIPADTILMVNAWAIQRDPKVWEDSTSFKPERF 420

Query: 426 QNG----GSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDM 485
           ++     GS  NN Y+FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKR+  KEIDM
Sbjct: 421 ESDQVREGSNNNNGYRFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDM 480

Query: 486 TEGQGISMPKVEPLEALCKARPIMKKLASD 510
           TEGQGISMPKVEPLEALC+ARPI+KKLA D
Sbjct: 481 TEGQGISMPKVEPLEALCRARPIIKKLALD 507

BLAST of Lag0002204 vs. NCBI nr
Match: XP_038907082.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 838.2 bits (2164), Expect = 3.8e-239
Identity = 418/507 (82.45%), Postives = 462/507 (91.12%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTK-NLPPSPLALPVIGHLHLLKHPIHRTL 65
           MET  TT+F +LI  FL + +IFL Q RK TTK NLPPSPLALP++GHLHLLKHPIHRTL
Sbjct: 1   MET--TTYFPVLIT-FLALISIFLFQNRKHTTKTNLPPSPLALPILGHLHLLKHPIHRTL 60

Query: 66  QTLSQKHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLG 125
            +L+Q HGPIFTLRLGSR VVVVSSTSAVEECFTTNDIVFANRP+FVSGKYL+YGN+TLG
Sbjct: 61  HSLAQNHGPIFTLRLGSRPVVVVSSTSAVEECFTTNDIVFANRPQFVSGKYLSYGNTTLG 120

Query: 126 AAPYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLF 185
           AAPYGDHWRNLRRLSATEVLSSIRLN SAGIR+EEI ILLRKL++VSG ++G+VKLK+ F
Sbjct: 121 AAPYGDHWRNLRRLSATEVLSSIRLNMSAGIRKEEIKILLRKLYKVSGTEYGKVKLKTPF 180

Query: 186 SELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQYT 245
           SELTFN+IMRMVAGKRYYGEEV+ELEEAKKFREIM+K+FQLGSYPGDFLPFLKWVD QY 
Sbjct: 181 SELTFNMIMRMVAGKRYYGEEVSELEEAKKFREIMQKSFQLGSYPGDFLPFLKWVDWQYA 240

Query: 246 KRVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVT 305
           KR+E+LG+D D FLQNLVDEHRR++G+GK+ NTMISH+LCLQKTQPDYYTDEIIKGLIVT
Sbjct: 241 KRLENLGKDMDLFLQNLVDEHRRNQGDGKQGNTMISHILCLQKTQPDYYTDEIIKGLIVT 300

Query: 306 ILSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVIS 365
           ILSAATETSS TIEWAMSNLLNHPE LNK K+ELDK IGQ+R ++E+DI NL YLQNVIS
Sbjct: 301 ILSAATETSSATIEWAMSNLLNHPEVLNKAKNELDKQIGQDRLLDEEDICNLPYLQNVIS 360

Query: 366 ETLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQ 425
           ETLRLYP APLLAPH SS SC LGGYHIP +T+LMVNAW +QRDPKVW+DSTSFKPERF+
Sbjct: 361 ETLRLYPPAPLLAPHFSSSSCCLGGYHIPANTMLMVNAWTIQRDPKVWEDSTSFKPERFE 420

Query: 426 N--GGSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEG 485
           +  GGS+ NN Y FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKR+  KEIDMTEG
Sbjct: 421 SEGGGSSNNNGYGFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEG 480

Query: 486 QGISMPKVEPLEALCKARPIMKKLASD 510
           QGISMPKVEPLEALC+ARPIMK LA D
Sbjct: 481 QGISMPKVEPLEALCRARPIMKNLALD 504

BLAST of Lag0002204 vs. NCBI nr
Match: XP_008459939.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 835.9 bits (2158), Expect = 1.9e-238
Identity = 423/510 (82.94%), Postives = 458/510 (89.80%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTK--NLPPSPLALPVIGHLHLLKHPIHRT 65
           MET    +F  LI   L + +IF +++ K  TK  NLPPSPL LP++GHLHLLKHPIHRT
Sbjct: 1   MET--NAYFPFLIT-LLALISIFFIRKSKTKTKRNNLPPSPLPLPILGHLHLLKHPIHRT 60

Query: 66  LQTLSQKHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTL 125
           L TLSQK+GPIFTLRLGSRLVVVVSSTSA EECFTTNDIVFANRPEFVSGKYLTYGNSTL
Sbjct: 61  LHTLSQKYGPIFTLRLGSRLVVVVSSTSAAEECFTTNDIVFANRPEFVSGKYLTYGNSTL 120

Query: 126 GAAPYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSL 185
           GAAPYGDHWRNLRRLSATEVLS+IRLN SA IR+EEI IL++KL+RVS  DFG+VKLKSL
Sbjct: 121 GAAPYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRVSSTDFGKVKLKSL 180

Query: 186 FSELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQY 245
           FSELTFN+IM MVAGKRYYGEEV+ELEEAKKFREIME+AFQLGSYPGDFLPFLKWVD QY
Sbjct: 181 FSELTFNMIMTMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQY 240

Query: 246 TKRVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIV 305
           TKRVESLG+DTD FLQNLVDEHR +E EG+K NTM+SHLL LQKTQPDYYTDEIIKGLIV
Sbjct: 241 TKRVESLGKDTDWFLQNLVDEHRSNEEEGEKGNTMVSHLLRLQKTQPDYYTDEIIKGLIV 300

Query: 306 TILSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVI 365
           TILSAATETSSVTIEWAMSNLLNHPEAL KVK+ELD  IGQ+R ++E+DI NL YLQNVI
Sbjct: 301 TILSAATETSSVTIEWAMSNLLNHPEALKKVKEELDTQIGQDRILDEEDISNLPYLQNVI 360

Query: 366 SETLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERF 425
           SETLRLYP APLLAPHLSS SCSLGGYHIP DTILMVNAWA+QRDPKVW+DSTSFKPERF
Sbjct: 361 SETLRLYPPAPLLAPHLSSSSCSLGGYHIPADTILMVNAWAIQRDPKVWEDSTSFKPERF 420

Query: 426 QNG----GSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDM 485
           ++     GS  NN Y+FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKR+  KEIDM
Sbjct: 421 ESDQVREGSNNNNGYRFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDM 480

Query: 486 TEGQGISMPKVEPLEALCKARPIMKKLASD 510
           TEGQGISMPKVEPLEALC+ARPI+KKLA D
Sbjct: 481 TEGQGISMPKVEPLEALCRARPIIKKLALD 507

BLAST of Lag0002204 vs. NCBI nr
Match: XP_023549077.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 803.9 bits (2075), Expect = 8.0e-229
Identity = 392/497 (78.87%), Postives = 446/497 (89.74%), Query Frame = 0

Query: 11  TTHFQLLIPFFLFIFTIFLLQRRKPTTKNLPPSPLALPVIGHLHLLKHPIHRTLQTLSQK 70
           T+ +   +  FL + +IFLLQRRK   KNLPP+P ALP++GHLHLLKHP+HRTLQ+L+QK
Sbjct: 3   TSSYVAALITFLAVGSIFLLQRRKNRAKNLPPTPWALPILGHLHLLKHPVHRTLQSLAQK 62

Query: 71  HGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGD 130
           HGPIFTLRLGSRL VVVSS  AVEECFTTNDIVFANRP+FVSG YL+YGN+T+GAAPYGD
Sbjct: 63  HGPIFTLRLGSRLAVVVSSAPAVEECFTTNDIVFANRPQFVSGMYLSYGNTTMGAAPYGD 122

Query: 131 HWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFSELTFN 190
           HWRNLRRLS+TE+LSSIRLN SAGIR+EE+ ILLRKL++ S  DFG+VKLK+LFSELTFN
Sbjct: 123 HWRNLRRLSSTEILSSIRLNMSAGIRKEEVNILLRKLYQFSRHDFGKVKLKTLFSELTFN 182

Query: 191 IIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQYTKRVESL 250
           IIMRMVAGKRYYG+EV ELEEAK+FR+IME +F+LGSYPGDFLPFLKWVDRQY KR++SL
Sbjct: 183 IIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPGDFLPFLKWVDRQYPKRMKSL 242

Query: 251 GRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAAT 310
           GR  D  LQNLVDEHRR + EGKK++TMISHLLCLQ++QP+YY+DEIIKGLIVTIL+AAT
Sbjct: 243 GRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSDEIIKGLIVTILTAAT 302

Query: 311 ETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISETLRLY 370
           ETSSVTIEWAMSNLLNHPEALNK + E+D  IGQ+R +EE+DIPNL YLQNVISETLRLY
Sbjct: 303 ETSSVTIEWAMSNLLNHPEALNKARAEVDAQIGQDRLLEEEDIPNLPYLQNVISETLRLY 362

Query: 371 PAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQNGGSAP 430
           PAAPLLAPH SS+SCSLGGYHIP  T L+VNAWAVQRDP+VW+D T FKPERF++GG A 
Sbjct: 363 PAAPLLAPHFSSESCSLGGYHIPAGTTLLVNAWAVQRDPEVWEDPTRFKPERFESGGVA- 422

Query: 431 NNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGISMPKV 490
            NAYKFLPFGLGRRACPGMGMANRVVGL LGSLIQSFEWKR+  +E+DMTE QGISM KV
Sbjct: 423 GNAYKFLPFGLGRRACPGMGMANRVVGLALGSLIQSFEWKRVSDEELDMTESQGISMYKV 482

Query: 491 EPLEALCKARPIMKKLA 508
           EPLEALC+ARPI+K LA
Sbjct: 483 EPLEALCRARPIIKNLA 498

BLAST of Lag0002204 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 4.2e-156
Identity = 275/509 (54.03%), Postives = 368/509 (72.30%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTKNLPPSP-LALPVIGHLHLLKHPIHRTL 65
           ME++   +  L +        +F + RR+   +NLPPSP LALPVIGHLHL+   +HR+L
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRR---RNLPPSPGLALPVIGHLHLIGKLLHRSL 60

Query: 66  QTLSQKHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLG 125
             LS+K+G +F+L+LG+RLV+VVSS +A EECFT NDIVFANRP F+ GKY+ Y  +T+ 
Sbjct: 61  YDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMV 120

Query: 126 AAPYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLF 185
            +PYG+HWRNLRRL+A E+ S+  LN    IR +E+  LL  L++ SG DFG+V++KS  
Sbjct: 121 GSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKL 180

Query: 186 SELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQL--GSYPGDFLPFLKWVD-R 245
           SEL+FN+ MRMVAGKRY+G++V + +EAK FR ++ + F+    S PGDF+PFL+W+D +
Sbjct: 181 SELSFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFK 240

Query: 246 QYTKRVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGL 305
            Y K+V  + ++ D FLQ L+ E R +    K   TMI HLL LQ++QP+YYTD+IIKG+
Sbjct: 241 NYEKKVSKISQEMDAFLQRLIHESRIN----KNNVTMIDHLLSLQESQPEYYTDQIIKGI 300

Query: 306 IVTILSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQN 365
           I+ +L A T+TS+VT+EWAMS LLNHPE L K + E++  +G  R +EE D+P L YL N
Sbjct: 301 IMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHN 360

Query: 366 VISETLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPE 425
           +ISET RL PAAP+L PH SSD C + GY +P  TIL+VNAWA+ RDP+ WD+ T FKPE
Sbjct: 361 IISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPE 420

Query: 426 RFQNGGSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTE 485
           R    G       K +PFG+GRR+CPG G+A RVVGLTLG+LIQ FEWKR+G  +IDM E
Sbjct: 421 RH---GGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAE 480

Query: 486 GQGISMPKVEPLEALCKARPIMKKLASDT 511
           G G++MPK +PLEALCK R I+ K+ S+T
Sbjct: 481 GSGLTMPKAQPLEALCKPRNILHKVVSET 498

BLAST of Lag0002204 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.1e-153
Identity = 279/499 (55.91%), Postives = 356/499 (71.34%), Query Frame = 0

Query: 11  TTHFQLLIPFFLFIFTIFLLQRRKPTTKNLPPSPLALPVIGHLHLLKHPIHRTLQTLSQK 70
           T +  L+I  F  I T+ +        KNLPP P  LP+IG+LH LK P+H T  TLSQK
Sbjct: 3   TFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQK 62

Query: 71  HGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGD 130
           +G IF+L  GSRLVVVVSS +  +ECFT NDIV ANRP F++GKY+ Y N+T+  +PYGD
Sbjct: 63  YGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGD 122

Query: 131 HWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFSELTFN 190
           HWRNLRR+ + E+LSS RLNS   IRR+EI  L++KL + S + F  V+L+ +FSE+TFN
Sbjct: 123 HWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFN 182

Query: 191 IIMRMVAGKRYYGE--EVAELEEAKKFREIMEKAFQLG--SYPGDFLPFLKWVDRQ-YTK 250
            IMRMV+GKRYYG   +V+++EEA+ FR I+++   LG  +  GDFL FL+W D     K
Sbjct: 183 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEK 242

Query: 251 RVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTI 310
           R++ + + TD FLQ L+DEHR  +   +  NTMI HLL  Q++QP+YYTD+IIKGL+V +
Sbjct: 243 RLKKISKRTDAFLQGLIDEHRFGK---RNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 302

Query: 311 LSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISE 370
           L A T+TSSVTIEWAMSNLLNHPE + K K+ELD HIG +R V+E DI  L YLQ+++ E
Sbjct: 303 LLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYE 362

Query: 371 TLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQN 430
           TLRL+ AAPLL PHLSS+  SLGGY+IP +TILMVNAW + RDP +W D T FKPERF+ 
Sbjct: 363 TLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK 422

Query: 431 GGSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGI 490
            G       K L FGLGRRACPG  ++ R  GLTLG LIQ FEWKR+G ++IDM E +GI
Sbjct: 423 EG----EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGI 482

Query: 491 SMPKVEPLEALCKARPIMK 505
           +  K   L A+CK R  +K
Sbjct: 483 TAGKKTSLNAMCKVRHPLK 494

BLAST of Lag0002204 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 531.9 bits (1369), Expect = 7.7e-150
Identity = 255/492 (51.83%), Postives = 362/492 (73.58%), Query Frame = 0

Query: 20  FFLFIFTIFLLQRRKPTTKNLPPSPLALPVIGHLHLLKHPIHRTLQTLSQKHGPIFTLRL 79
           F+L +F IF +  R    KNLPP P +LP+IG+LH LK P+HRT + LS+K+G +F+L  
Sbjct: 12  FYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWF 71

Query: 80  GSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHWRNLRRLS 139
           GSRLVVVVSS S  ++CFT ND+V ANRP F+SGKY+ Y  +TLG+  YG+HWRNLRR++
Sbjct: 72  GSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRIT 131

Query: 140 ATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFSELTFNIIMRMVAGK 199
           A +VLS+ R+NS +GIRR+E   L+ +L   S  +F  ++L S   ++TFN IMRM++GK
Sbjct: 132 ALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGK 191

Query: 200 RYYGE--EVAELEEAKKFREIMEKAFQL--GSYPGDFLPFLKWVD-RQYTKRVESLGRDT 259
           RYYGE  + ++L+EA +FR+++ +  QL   +   DF+P L+++D     KR++ +   T
Sbjct: 192 RYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKT 251

Query: 260 DGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAATETSS 319
           D FL+ L++EHR  +   ++ NTMI HLL LQ +QP+YYTD+IIKGL + +L A T++S+
Sbjct: 252 DAFLRGLIEEHRTKK---ERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSA 311

Query: 320 VTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISETLRLYPAAP 379
           VT+EW+MSNLLNHPE L KVKDELD H+GQ+R V+E D+P L YL+NVI+ETLRLY  AP
Sbjct: 312 VTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAP 371

Query: 380 LLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQNGGSAPNNAY 439
           LL PH +SD C++GGY +P DTI+++NAWA+ RDP++W ++T+FKPERF+  G       
Sbjct: 372 LLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE---- 431

Query: 440 KFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGISMPKVEPLE 499
           K + FG+GRRACPG G+A R + +TL  LIQ F+WK + G +ID+ E  G ++ K+ PL+
Sbjct: 432 KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLK 491

Query: 500 ALCKARPIMKKL 507
           A+CK+RP++ K+
Sbjct: 492 AMCKSRPVINKV 496

BLAST of Lag0002204 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.8e-144
Identity = 248/492 (50.41%), Postives = 353/492 (71.75%), Query Frame = 0

Query: 20  FFLFIFTIFLLQRRKPTTKNLPPSPLALPVIGHLHLLKHPIHRTLQTLSQKHGPIFTLRL 79
           F+L IF I  L  +    KNLPP P +LP+IG+LH LK P++RT + L++K+G + +L  
Sbjct: 12  FYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWF 71

Query: 80  GSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHWRNLRRLS 139
           GSRLVVVVSS S  +ECFT ND+V ANRP F+SGKY+ Y  +TLG+  YG+HWRNLRR++
Sbjct: 72  GSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRIT 131

Query: 140 ATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFSELTFNIIMRMVAGK 199
           + +VLS+ R+N+ A IRR+E   L++KL   S   F  V+L   F ++TFN IMRM++GK
Sbjct: 132 SLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMISGK 191

Query: 200 RYYGE--EVAELEEAKKFREIMEKAFQL--GSYPGDFLPFLKWVD-RQYTKRVESLGRDT 259
           RYYG+  +++E++EA +FR+++ +  QL   +   DF+P LK++D     KRV+ +G   
Sbjct: 192 RYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIGEKN 251

Query: 260 DGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAATETSS 319
           D FL  L+ E R    + ++ NTMI HLL +Q++QP+YYTD IIKGL + +L A T++S+
Sbjct: 252 DVFLSGLLQEQR---SKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAGTDSSA 311

Query: 320 VTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISETLRLYPAAP 379
           VT+EW MSN+LN+PE L KV+DE+D H+GQ+R V+E D+P L YL+NVI ETLRLY  AP
Sbjct: 312 VTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLYTPAP 371

Query: 380 LLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQNGGSAPNNAY 439
           LL PH ++D C +GGY +P DTI+++NAWA+ RDP+ W ++T+FKPERF   G       
Sbjct: 372 LLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKGELE---- 431

Query: 440 KFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGISMPKVEPLE 499
           K + FG+GRRACPG G+A R + +TL  L+Q F+WKR+  ++IDM+E  G +M K+ PL+
Sbjct: 432 KMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKLLPLK 491

Query: 500 ALCKARPIMKKL 507
           A+CK RP++ K+
Sbjct: 492 AMCKTRPVVNKV 496

BLAST of Lag0002204 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.7e-144
Identity = 259/489 (52.97%), Postives = 345/489 (70.55%), Query Frame = 0

Query: 23  FIFTI-FLLQRRKPTTKNLPPSPLALPVIGHLHLLKHPIHRTLQTLSQKHGPIFTLRLGS 82
           FI TI  LL+      KNLPP P  +P+IG+LH LKHP+HRT  TLSQ +G IF+L  GS
Sbjct: 12  FIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGS 71

Query: 83  RLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHWRNLRRLSAT 142
           RLVVVVSS S   ECFT NDI+ ANRP F++GKY+ Y  +TLG+A YGDHWRNLRR++  
Sbjct: 72  RLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTI 131

Query: 143 EVLSSIRLNSSAGIRREEIGILLRKLHR---VSGDDFGRVKLKSLFSELTFNIIMRMVAG 202
           +VLS+ RLNS  G+RR+E   L++KL +     G  F +V+L+   +E+TFN +MRM++G
Sbjct: 132 DVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMISG 191

Query: 203 KRYYGE--EVAELEEAKKFREIMEKAFQL--GSYPGDFLPFLKWVD-RQYTKRVESLGRD 262
           KRYYG+  +V+++EEAK+FREI+ +   L   +  GDFLP L+ VD     KR + + + 
Sbjct: 192 KRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIAKR 251

Query: 263 TDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAATETS 322
           ++ FL+ L++EHRR         TMI HLL L ++QP+YY+D +IKGLI  +L A T+TS
Sbjct: 252 SNAFLEGLIEEHRRGNIHSDG-GTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTS 311

Query: 323 SVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISETLRLYPAA 382
           +VTIEW MS LLNHPE L K K+ELD  IG+ + V+E D+  L YLQN+ISETLRL+P A
Sbjct: 312 AVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPA 371

Query: 383 PLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQNGGSAPNNA 442
           PLL PH SS+ C++G +++P DTI++ N W + RDPK W+D+ SFKPERF+         
Sbjct: 372 PLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEK----EEEV 431

Query: 443 YKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGISMPKVEPL 502
            K + FGLGRRACPG+ +A R VG T+G LIQ FEW+R   +++DM EG+GI+MP   PL
Sbjct: 432 NKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPL 491

BLAST of Lag0002204 vs. ExPASy TrEMBL
Match: A0A0A0K9E5 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088180 PE=2 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 2.3e-242
Identity = 425/510 (83.33%), Postives = 461/510 (90.39%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTKNLPPSPLALPVIGHLHLLKHPIHRTLQ 65
           ME    T+   LI  FL + +IF +Q+   T KNLPPSPLALP++GHLHLLKHPIHRTL 
Sbjct: 1   MEMEAITYLPFLIT-FLALVSIFFIQK---TKKNLPPSPLALPILGHLHLLKHPIHRTLH 60

Query: 66  TLSQKHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGA 125
            LSQK+GPIFTLRLGSRLVVVVSS SA+EECFTTNDIVFANRPEFVSGKYLTYGNSTLGA
Sbjct: 61  NLSQKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGA 120

Query: 126 APYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFS 185
           APYGDHWRNLRRLSATE+LS+IRLN SA IR+EEIGIL++KL+RV G +FG+VKLKSLFS
Sbjct: 121 APYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFS 180

Query: 186 ELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQYTK 245
           ELTFNIIMRM+AGKRYYGEEV+ELEEAKKFREIME+AFQLGSYPGDFLPFLKWVD QYTK
Sbjct: 181 ELTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTK 240

Query: 246 RVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTI 305
           RVESLG+DTD FLQNLVDEHRR+E EG+K NTMISHLLCLQKTQPDYYTDEIIKGLIVTI
Sbjct: 241 RVESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTI 300

Query: 306 LSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISE 365
           LSAATETSSVTIEWAMSNLLNHPEAL KVKDELDK IGQ+R ++E+DI NL YLQNVISE
Sbjct: 301 LSAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISE 360

Query: 366 TLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQN 425
           TLRLYP APLLAPHLSS SCSLGGYHIP DT+LMVNAWA+QRDPKVW+DSTSFKPERF++
Sbjct: 361 TLRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFES 420

Query: 426 ------GGSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDM 485
                 G +  NN Y FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKR+  KEIDM
Sbjct: 421 DHQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDM 480

Query: 486 TEGQGISMPKVEPLEALCKARPIMKKLASD 510
           TEGQGISMPKVEPLEALC+ARPI+KKLA D
Sbjct: 481 TEGQGISMPKVEPLEALCRARPIIKKLALD 506

BLAST of Lag0002204 vs. ExPASy TrEMBL
Match: A0A5A7TCR6 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001720 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.9e-239
Identity = 424/510 (83.14%), Postives = 459/510 (90.00%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTK--NLPPSPLALPVIGHLHLLKHPIHRT 65
           MET    +F  LI   L + +IF +++ K  TK  NLPPSPL LP++GHLHLLKHPIHRT
Sbjct: 1   MET--NAYFPFLIT-LLALISIFFIRKSKTKTKRNNLPPSPLPLPILGHLHLLKHPIHRT 60

Query: 66  LQTLSQKHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTL 125
           L TLSQK+GPIFTLRLGSRLVVVVSSTSA EECFTTNDIVFANRPEFVSGKYLTYGNSTL
Sbjct: 61  LHTLSQKYGPIFTLRLGSRLVVVVSSTSAAEECFTTNDIVFANRPEFVSGKYLTYGNSTL 120

Query: 126 GAAPYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSL 185
           GAAPYGDHWRNLRRLSATEVLS+IRLN SA IR+EEI IL++KL+RVS  DFG+VKLKSL
Sbjct: 121 GAAPYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRVSSTDFGKVKLKSL 180

Query: 186 FSELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQY 245
           FSELTFN+IMRMVAGKRYYGEEV+ELEEAKKFREIME+AFQLGSYPGDFLPFLKWVD QY
Sbjct: 181 FSELTFNMIMRMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQY 240

Query: 246 TKRVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIV 305
           TKRVESLG+DTD FLQNLVDEHR +E EG+K NTM+SHLL LQKTQPDYYTDEIIKGLIV
Sbjct: 241 TKRVESLGKDTDWFLQNLVDEHRSNEEEGEKGNTMVSHLLRLQKTQPDYYTDEIIKGLIV 300

Query: 306 TILSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVI 365
           TILSAATETSSVTIEWAMSNLLNHPEAL KVK+ELD  IGQ+R ++E+DI NL YLQNVI
Sbjct: 301 TILSAATETSSVTIEWAMSNLLNHPEALKKVKEELDTQIGQDRILDEEDISNLPYLQNVI 360

Query: 366 SETLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERF 425
           SETLRLYP APLLAPHLSS SCSLGGYHIP DTILMVNAWA+QRDPKVW+DSTSFKPERF
Sbjct: 361 SETLRLYPPAPLLAPHLSSSSCSLGGYHIPADTILMVNAWAIQRDPKVWEDSTSFKPERF 420

Query: 426 QNG----GSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDM 485
           ++     GS  NN Y+FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKR+  KEIDM
Sbjct: 421 ESDQVREGSNNNNGYRFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDM 480

Query: 486 TEGQGISMPKVEPLEALCKARPIMKKLASD 510
           TEGQGISMPKVEPLEALC+ARPI+KKLA D
Sbjct: 481 TEGQGISMPKVEPLEALCRARPIIKKLALD 507

BLAST of Lag0002204 vs. ExPASy TrEMBL
Match: A0A1S3CBC7 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103498908 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 9.2e-239
Identity = 423/510 (82.94%), Postives = 458/510 (89.80%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTK--NLPPSPLALPVIGHLHLLKHPIHRT 65
           MET    +F  LI   L + +IF +++ K  TK  NLPPSPL LP++GHLHLLKHPIHRT
Sbjct: 1   MET--NAYFPFLIT-LLALISIFFIRKSKTKTKRNNLPPSPLPLPILGHLHLLKHPIHRT 60

Query: 66  LQTLSQKHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTL 125
           L TLSQK+GPIFTLRLGSRLVVVVSSTSA EECFTTNDIVFANRPEFVSGKYLTYGNSTL
Sbjct: 61  LHTLSQKYGPIFTLRLGSRLVVVVSSTSAAEECFTTNDIVFANRPEFVSGKYLTYGNSTL 120

Query: 126 GAAPYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSL 185
           GAAPYGDHWRNLRRLSATEVLS+IRLN SA IR+EEI IL++KL+RVS  DFG+VKLKSL
Sbjct: 121 GAAPYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRVSSTDFGKVKLKSL 180

Query: 186 FSELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQY 245
           FSELTFN+IM MVAGKRYYGEEV+ELEEAKKFREIME+AFQLGSYPGDFLPFLKWVD QY
Sbjct: 181 FSELTFNMIMTMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQY 240

Query: 246 TKRVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIV 305
           TKRVESLG+DTD FLQNLVDEHR +E EG+K NTM+SHLL LQKTQPDYYTDEIIKGLIV
Sbjct: 241 TKRVESLGKDTDWFLQNLVDEHRSNEEEGEKGNTMVSHLLRLQKTQPDYYTDEIIKGLIV 300

Query: 306 TILSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVI 365
           TILSAATETSSVTIEWAMSNLLNHPEAL KVK+ELD  IGQ+R ++E+DI NL YLQNVI
Sbjct: 301 TILSAATETSSVTIEWAMSNLLNHPEALKKVKEELDTQIGQDRILDEEDISNLPYLQNVI 360

Query: 366 SETLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERF 425
           SETLRLYP APLLAPHLSS SCSLGGYHIP DTILMVNAWA+QRDPKVW+DSTSFKPERF
Sbjct: 361 SETLRLYPPAPLLAPHLSSSSCSLGGYHIPADTILMVNAWAIQRDPKVWEDSTSFKPERF 420

Query: 426 QNG----GSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDM 485
           ++     GS  NN Y+FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKR+  KEIDM
Sbjct: 421 ESDQVREGSNNNNGYRFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDM 480

Query: 486 TEGQGISMPKVEPLEALCKARPIMKKLASD 510
           TEGQGISMPKVEPLEALC+ARPI+KKLA D
Sbjct: 481 TEGQGISMPKVEPLEALCRARPIIKKLALD 507

BLAST of Lag0002204 vs. ExPASy TrEMBL
Match: A0A6J1GPQ4 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111456346 PE=3 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.9e-229
Identity = 392/496 (79.03%), Postives = 446/496 (89.92%), Query Frame = 0

Query: 11  TTHFQLLIPFFLFIFTIFLLQRRKPTTKNLPPSPLALPVIGHLHLLKHPIHRTLQTLSQK 70
           T+ +   +  FL + +IFLLQ+RK   KNLPP+P ALP++GHLHLLKHP+HRTLQ+L+QK
Sbjct: 3   TSSYVAALITFLAVGSIFLLQKRKNRAKNLPPTPWALPILGHLHLLKHPVHRTLQSLAQK 62

Query: 71  HGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGD 130
           HGPIFTLRLGSRL VVVSS  AVEECFTTNDIVFANRP+FVSG YL+YGN+T+GAAPYGD
Sbjct: 63  HGPIFTLRLGSRLAVVVSSAPAVEECFTTNDIVFANRPQFVSGMYLSYGNTTMGAAPYGD 122

Query: 131 HWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFSELTFN 190
           HWRNLRRLS+TE+LSSIRLN SAGIR+EE+ ILLRKL++ S  DFG+VKLK+LFSELTFN
Sbjct: 123 HWRNLRRLSSTEILSSIRLNMSAGIRKEEVNILLRKLYQFSRHDFGKVKLKTLFSELTFN 182

Query: 191 IIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQYTKRVESL 250
           IIMRMVAGKRYYG+EV ELEEAK+FR+IME +F+LGSYPGDFLPFLKWVDRQY KR++SL
Sbjct: 183 IIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPGDFLPFLKWVDRQYPKRMKSL 242

Query: 251 GRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAAT 310
           GR  D  LQNLVDEHRR + EGKK++TMISHLLCLQ++QP+YY+DEIIKGLIVTIL+AAT
Sbjct: 243 GRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSDEIIKGLIVTILTAAT 302

Query: 311 ETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISETLRLY 370
           ETSSVTIEWAMSNLLNHPEALNK + ELD  IGQ+R +EE+DIPNL YLQNVISETLRLY
Sbjct: 303 ETSSVTIEWAMSNLLNHPEALNKARAELDAQIGQDRLLEEEDIPNLPYLQNVISETLRLY 362

Query: 371 PAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQNGGSAP 430
           PAAPLLAPH SS+SCSLGGYHIP  T L+VNAWAVQRDP+VW+D TSFKPERF++GG A 
Sbjct: 363 PAAPLLAPHFSSESCSLGGYHIPAGTTLLVNAWAVQRDPEVWEDPTSFKPERFESGGVAA 422

Query: 431 NNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGISMPKV 490
            NAYKFLPFGLGRRACPGMGMANRVVGL LGSLIQSFEWKR+  +E+DMTE QGISM KV
Sbjct: 423 -NAYKFLPFGLGRRACPGMGMANRVVGLALGSLIQSFEWKRVSDEELDMTESQGISMYKV 482

Query: 491 EPLEALCKARPIMKKL 507
           EPLEALC+ARPI+K L
Sbjct: 483 EPLEALCRARPIIKNL 497

BLAST of Lag0002204 vs. ExPASy TrEMBL
Match: A0A6J1JR43 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111488144 PE=3 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.5e-228
Identity = 394/502 (78.49%), Postives = 449/502 (89.44%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTKNLPPSPLALPVIGHLHLLKHPIHRTLQ 65
           MET  +++F  L+   L + +IFLLQ+RK   KNLPPSP ALP++GHLHLLKHP+HRTLQ
Sbjct: 1   MET--SSYFAALVT-LLALGSIFLLQKRKNRAKNLPPSPWALPILGHLHLLKHPVHRTLQ 60

Query: 66  TLSQKHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGA 125
           +L+QKHGPIFTLRLGSRL +VVSS  AVEECFTTNDIVFANRP+FVSG YL+YGN+T+GA
Sbjct: 61  SLAQKHGPIFTLRLGSRLAIVVSSAPAVEECFTTNDIVFANRPQFVSGMYLSYGNTTMGA 120

Query: 126 APYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFS 185
           APYGDHWRNLRRLS+TE+LSSIRLN SAGIR+EE+ ILLRKL++ S  DFG+VKLK+LFS
Sbjct: 121 APYGDHWRNLRRLSSTEILSSIRLNMSAGIRKEEVNILLRKLYQFSRHDFGKVKLKTLFS 180

Query: 186 ELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDRQYTK 245
           ELTFNIIMRMVAGKRY+G+EV ELEEAK+FREIME +F+LGSYPGDFLPFLKWVDRQY K
Sbjct: 181 ELTFNIIMRMVAGKRYFGDEVTELEEAKEFREIMETSFRLGSYPGDFLPFLKWVDRQYPK 240

Query: 246 RVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTI 305
           R++SLGR  D  LQNLVDEHRR + EGKK++TMISHLLCLQ++QP+YY+DEIIKGLIVTI
Sbjct: 241 RMKSLGRALDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSDEIIKGLIVTI 300

Query: 306 LSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISE 365
           L+AATETSSVTIEWAMSNLLNHPEALNK + ELD  IGQ+R +EE+DIP L YLQNVISE
Sbjct: 301 LTAATETSSVTIEWAMSNLLNHPEALNKARAELDAQIGQDRLLEEEDIPTLPYLQNVISE 360

Query: 366 TLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQN 425
           TLRLYPAAPLLAPH SS+SCSLGGYHIP  T ++VNAWAVQRDP+VW+D T FKPERF++
Sbjct: 361 TLRLYPAAPLLAPHFSSESCSLGGYHIPAGTTMLVNAWAVQRDPEVWEDPTRFKPERFES 420

Query: 426 GGSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGI 485
           GG A  NAYKFLPFGLGRRACPGMGMANRVVGL LGSLIQSFEWKR+  +E+DMTE QGI
Sbjct: 421 GGVAA-NAYKFLPFGLGRRACPGMGMANRVVGLALGSLIQSFEWKRVSDEELDMTESQGI 480

Query: 486 SMPKVEPLEALCKARPIMKKLA 508
           SM KVEPLEALC+ARPIMK LA
Sbjct: 481 SMYKVEPLEALCRARPIMKNLA 498

BLAST of Lag0002204 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 537.0 bits (1382), Expect = 1.7e-152
Identity = 270/501 (53.89%), Postives = 363/501 (72.46%), Query Frame = 0

Query: 16  LLIPFFLFIFTIFLLQRRKPTTKNLPPSPL-ALPVIGHLHLLKHPIHRTLQTLSQKHG-- 75
           L+  F   + ++  L  R     NLPPSP  ALPVIGHL LLK P+HR   ++SQ  G  
Sbjct: 6   LIFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDA 65

Query: 76  PIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHW 135
           PI +LRLG+RL+ VVSS S  EECFT ND++ ANR   +S K+++YGNST+ +A Y +HW
Sbjct: 66  PIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHW 125

Query: 136 RNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFSELTFNII 195
           RNLRR+ A E+ S+ RLNS + IRR+EI  L+ +L R S   F +V++KS+FS+LTFN I
Sbjct: 126 RNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNI 185

Query: 196 MRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPG---DFLPFLKWVDRQYTKRVES 255
           +RM+AGK YYG+   +  EAK+ R ++ +A    S PG   D++P L W+    T R++ 
Sbjct: 186 IRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMS-SSGPGNAADYIPILTWITYSET-RIKK 245

Query: 256 LGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA 315
           L    D FLQ LVDE R  EG+ KK NTM+ HLLCLQ+TQP+YY D IIKG ++++++  
Sbjct: 246 LAGRLDEFLQGLVDEKR--EGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGG 305

Query: 316 TETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISETLRL 375
           T+T++VT+EWA+S+LLN+PE LNK +DE+D+ IG +R +EE DIPNL YLQN++SETLRL
Sbjct: 306 TDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRL 365

Query: 376 YPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQNGGSA 435
           YPAAP+L PH++S  C +GGY +P  T+L+ NAWA+ RDP +WDD TSFKPERF+  G A
Sbjct: 366 YPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEGEA 425

Query: 436 PNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGISMPK 495
                K +PFGLGRRACPG G+A R+V L+LGSLIQ FEW+R+G +E+DMTEG G++MPK
Sbjct: 426 K----KLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPK 485

Query: 496 VEPLEALCKARPIMKKLASDT 511
             PLEA+C+AR  + K+  D+
Sbjct: 486 ARPLEAMCRARDFVGKILPDS 498

BLAST of Lag0002204 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 528.1 bits (1359), Expect = 7.9e-150
Identity = 273/508 (53.74%), Postives = 365/508 (71.85%), Query Frame = 0

Query: 15  QLLIPFFLFI-----FTIFLLQRRKPTTKNLPPSP-LALPVIGHLHLLKHPIHRTLQTLS 74
           Q LI  FLFI     F I  ++RR     NLPPSP  ALPVIGHL LLK P+HR   ++S
Sbjct: 4   QTLIFTFLFISLSLTFIIGRIKRR----PNLPPSPSWALPVIGHLRLLKPPLHRVFLSVS 63

Query: 75  QKHG--PIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 134
           +  G  PI +LRLG+RLV VVSS S  EECFT ND+V ANR   ++ K+++YG +T+  A
Sbjct: 64  ESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTA 123

Query: 135 PYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFSE 194
            YGDHWRNLRR+ A E+ S+ RLNS + IRR+EI  L+  L R S  +F +V++KS+FS 
Sbjct: 124 SYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSN 183

Query: 195 LTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEK---AFQLGSYPGDFLPFLKWVDRQY 254
           LTFN I+RM+AGK YYG+   +  EAK+ RE++ +    F  G+   D+LP L W+    
Sbjct: 184 LTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGN-TADYLPILTWITGS- 243

Query: 255 TKRVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIV 314
            KR++ +    D FLQ LVDE R  EG+ K+ NTM+ HLLCLQ+TQP+YYTD IIKG+++
Sbjct: 244 EKRIKKIASRLDEFLQGLVDERR--EGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIML 303

Query: 315 TILSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVI 374
           +++ A T+TS+VT+EW +S LLNHP+ L+K +DE+D  +G  R VEE D+ +L YLQN++
Sbjct: 304 SLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIV 363

Query: 375 SETLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERF 434
           SE+LRLYPA+PLL PH++S+ C +GGYH+P  T+L+ NAWA+ RDPK+WDD TSFKPERF
Sbjct: 364 SESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERF 423

Query: 435 QNGGSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTE-G 494
           +  G     A K L FGLGRRACPG G+A R+  LT+GSLIQ FEW+R+G +E+DMTE G
Sbjct: 424 EKEG----EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGG 483

Query: 495 QGISMPKVEPLEALCKARPIMKKLASDT 511
            G+ MPK  PL A+CKARP++ K+ +++
Sbjct: 484 GGVIMPKAIPLVAMCKARPVVGKILNES 499

BLAST of Lag0002204 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 523.1 bits (1346), Expect = 2.5e-148
Identity = 271/511 (53.03%), Postives = 365/511 (71.43%), Query Frame = 0

Query: 6   METAITTHFQLLIPFFLFIFTIFLLQRRKPTTKNLPPSPL-ALPVIGHLHLLKHPIHRTL 65
           MET  T  F +L      I+ I  L +RKP   NLPPSP  +LPVIGHL LLK PIHRT 
Sbjct: 1   METK-TLIFSILFVVLSLIYLIGKL-KRKP---NLPPSPAWSLPVIGHLRLLKPPIHRTF 60

Query: 66  QTLSQ--KHGPIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNST 125
            +LSQ   + PIF+LRLG+RLV V SS S  EECFT ND+V ANRP F+  K++ Y  +T
Sbjct: 61  LSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTT 120

Query: 126 LGAAPYGDHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKS 185
           + AA YGDHWRNLRR+ + E+ S+ RLNS   IR++EI  L+ +L R    +F +V +KS
Sbjct: 121 MIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKS 180

Query: 186 LFSELTFNIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPG--DFLPFLKWVD 245
           + S+LTFN I+RMVAGKRYYG+ V +  EAK+ R+++             D+LP L+ V 
Sbjct: 181 MLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVS 240

Query: 246 RQYTKRVESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKG 305
             Y  RV+ L    D FLQ LVDE R  + +G   NTMI HLL LQ++QPDY+TD IIKG
Sbjct: 241 -DYETRVKKLAGRLDEFLQGLVDEKREAKEKG---NTMIDHLLTLQESQPDYFTDRIIKG 300

Query: 306 LIVTILSAATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQ 365
            ++ ++ A T+TS+VT+EWA+SN+LNHP+ LNK +DE+D+ IG +R ++E DI NL YLQ
Sbjct: 301 NMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQ 360

Query: 366 NVISETLRLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKP 425
           N++SETLRLYPAAP+L PH++S+ C + GY +P  TIL+ N WA+ RDP++WDD  SFKP
Sbjct: 361 NIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKP 420

Query: 426 ERFQNGGSAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMT 485
           ERF+  G     A K +PFGLGRRACPG G+A+R++ LTLGSLIQ  EW+++ G+E+DM+
Sbjct: 421 ERFEKEG----EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI-GEEVDMS 480

Query: 486 EGQGISMPKVEPLEALCKARPIMKKLASDTI 512
           EG+G++MPK +PLEA+C+ARP + K+ ++++
Sbjct: 481 EGKGVTMPKAKPLEAMCRARPSVVKIFNESV 497

BLAST of Lag0002204 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 508.1 bits (1307), Expect = 8.5e-144
Identity = 267/499 (53.51%), Postives = 352/499 (70.54%), Query Frame = 0

Query: 15  QLLIPFFLFIFT--IFLLQRRKPTTKNLPPSP-LALPVIGHLHLLKHPIHRTLQTLSQKH 74
           Q+LI  FL +FT  IFLL R K    NLPPSP ++LPVIGHLHLLK P+HRT  +LS+  
Sbjct: 5   QILILSFLSLFTLAIFLLTRSK-RKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSI 64

Query: 75  G--PIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYG 134
           G  P+F LRLG+RLV V+SS S  EECFT ND+V ANRP+F   K+L Y  + L +A YG
Sbjct: 65  GNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYG 124

Query: 135 DHWRNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFSELTF 194
           DHWRNLRR++A E+ S+ RLNS   IR++EI  L+  L R S   F  V++K+L + L  
Sbjct: 125 DHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLAS 184

Query: 195 NIIMRMVAGKRYYGEEVAELEEAKKFREIMEKAFQL--GSYPGDFLPFLKWVDRQYTKRV 254
           N  +RM+AGKRY+GE   + ++AK  + ++ +A        P D+L  L+WV   Y KR+
Sbjct: 185 NTTIRMLAGKRYFGE---DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWVS-SYEKRI 244

Query: 255 ESLGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTILS 314
           ++LG   D FLQ LVDE R ++ +G+   TMI HLL LQ  QPDYYTD IIKG+I+T++ 
Sbjct: 245 KNLGNRFDTFLQKLVDEKRAEKEKGE---TMIDHLLALQDIQPDYYTDVIIKGIILTLII 304

Query: 315 AATETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISETL 374
           A T+TSSVT+EWAMSNLLNHPE L K + E+D+ +G +R V+E DI NL YLQ+++ ETL
Sbjct: 305 AGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETL 364

Query: 375 RLYPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQNGG 434
           R+YPA PLL PHLSS+ C +GGY IP  T+++ NAWA+ RDP+VW+D   FKPERF+  G
Sbjct: 365 RMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEG 424

Query: 435 SAPNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGISM 494
            A     K + FG+GRRACPG G+A+R++   LGSL+Q FEW+R+G   +DMTE +G ++
Sbjct: 425 EAE----KLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATL 484

Query: 495 PKVEPLEALCKARPIMKKL 507
           PK  PL A+CKAR I+ KL
Sbjct: 485 PKAIPLRAMCKARSIVDKL 491

BLAST of Lag0002204 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 503.8 bits (1296), Expect = 1.6e-142
Identity = 260/496 (52.42%), Postives = 340/496 (68.55%), Query Frame = 0

Query: 16  LLIPFFLFIFTIFLLQRRKPTTKNLPPSP-LALPVIGHLHLLKHPIHRTLQTLSQKHG-- 75
           LL+ F     +I LL  +     NLPPSP   LPVIGHLHLLK P+HRT  ++S+  G  
Sbjct: 7   LLLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNA 66

Query: 76  PIFTLRLGSRLVVVVSSTSAVEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHW 135
           PIF LRLG+RLV V+SS S  EECFT ND+V ANRP+ +  K++ Y  + + AA YGDHW
Sbjct: 67  PIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHW 126

Query: 136 RNLRRLSATEVLSSIRLNSSAGIRREEIGILLRKLHRVSGDDFGRVKLKSLFSELTFNII 195
           RNLRR++A E+ SS R+++ + IR++EI  L+  L R S   F  V+LKSL + L FN I
Sbjct: 127 RNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNI 186

Query: 196 MRMVAGKRYYGEEVAELEEAKKFREIMEKAFQLGSYPG---DFLPFLKWVDRQYTKRVES 255
           + MVAGKRYYG    + +EAK  RE++ +    G+  G   D+LP + WV   +  + + 
Sbjct: 187 IMMVAGKRYYGTGTEDNDEAKLVRELIAE-IMAGAGSGNLADYLPSINWV-TNFENQTKI 246

Query: 256 LGRDTDGFLQNLVDEHRRDEGEGKKMNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA 315
           LG   D  LQ LVDE R ++ +G+   T+I HLL  Q+T+P+YYTD IIKG+I+ ++ A 
Sbjct: 247 LGNRLDRVLQKLVDEKRAEKEKGQ---TLIDHLLSFQETEPEYYTDVIIKGIILALVLAG 306

Query: 316 TETSSVTIEWAMSNLLNHPEALNKVKDELDKHIGQERFVEEDDIPNLQYLQNVISETLRL 375
           T+TSSVT+EWAMSNLLNHPE L K + E+D  IG +R VEE DI NL YLQN++SETLRL
Sbjct: 307 TDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRL 366

Query: 376 YPAAPLLAPHLSSDSCSLGGYHIPPDTILMVNAWAVQRDPKVWDDSTSFKPERFQNGGSA 435
           YPA PLL PH SSD C + GY +P  T+L+ N WA+ RDP +W++   FKPERF+  G  
Sbjct: 367 YPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKEG-- 426

Query: 436 PNNAYKFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRLGGKEIDMTEGQGISMPK 495
              A K +PFG+GRRACPG  +  R+V L LG LIQSFEW+R+G + +DMTEG+GI+MPK
Sbjct: 427 --EARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPK 486

Query: 496 VEPLEALCKARPIMKK 506
             PL A+CKAR I+ K
Sbjct: 487 ATPLRAMCKARAIVGK 493