Lag0002155 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002155
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	S-protein homolog
Location	chr4: 39975485 .. 39975925 (+)
RNA-Seq Expression	Lag0002155
Synteny	Lag0002155

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGTTCTCAAAAATCCATTGAGCTTCATCACAATCGTGGGTTTGGTCCTTGTTGGTTCTTCCTCGGTAGCTTTAGGAGGATGGCCACTCCCCAAATGGCGTGTAGACATTGTGAATGAGCTTAGCAACGGACAAACACTATTCGTTCATTGCAAGTCCAAGGATGATGATTTGGGACAACACAAACTCAAAAGTGGAACTAAGTATGGTTTTAATTTCAAAGAAAACGTTTGGGGAACCACACTATTTTGGTGTTCCTTGCGTAAACCAGACAATGCCCGTGTTGCTTTTGATGTGTATTGGTTTGATGCTAGCACAAGTCATTGGCTTTACACAAGATGCAGCTGGAAAAATTGCATTTGGATTGCTAAAGTAGATGGGATTTACATTAAAAATATCCCTTTGAATCAAGATGAGCTGGTTCATACGTGGAAATGA

mRNA sequence

ATGGGAGTTCTCAAAAATCCATTGAGCTTCATCACAATCGTGGGTTTGGTCCTTGTTGGTTCTTCCTCGGTAGCTTTAGGAGGATGGCCACTCCCCAAATGGCGTGTAGACATTGTGAATGAGCTTAGCAACGGACAAACACTATTCGTTCATTGCAAGTCCAAGGATGATGATTTGGGACAACACAAACTCAAAAGTGGAACTAAGTATGGTTTTAATTTCAAAGAAAACGTTTGGGGAACCACACTATTTTGGTGTTCCTTGCGTAAACCAGACAATGCCCGTGTTGCTTTTGATGTGTATTGGTTTGATGCTAGCACAAGTCATTGGCTTTACACAAGATGCAGCTGGAAAAATTGCATTTGGATTGCTAAAGTAGATGGGATTTACATTAAAAATATCCCTTTGAATCAAGATGAGCTGGTTCATACGTGGAAATGA

Coding sequence (CDS)

ATGGGAGTTCTCAAAAATCCATTGAGCTTCATCACAATCGTGGGTTTGGTCCTTGTTGGTTCTTCCTCGGTAGCTTTAGGAGGATGGCCACTCCCCAAATGGCGTGTAGACATTGTGAATGAGCTTAGCAACGGACAAACACTATTCGTTCATTGCAAGTCCAAGGATGATGATTTGGGACAACACAAACTCAAAAGTGGAACTAAGTATGGTTTTAATTTCAAAGAAAACGTTTGGGGAACCACACTATTTTGGTGTTCCTTGCGTAAACCAGACAATGCCCGTGTTGCTTTTGATGTGTATTGGTTTGATGCTAGCACAAGTCATTGGCTTTACACAAGATGCAGCTGGAAAAATTGCATTTGGATTGCTAAAGTAGATGGGATTTACATTAAAAATATCCCTTTGAATCAAGATGAGCTGGTTCATACGTGGAAATGA

Protein sequence

MGVLKNPLSFITIVGLVLVGSSSVALGGWPLPKWRVDIVNELSNGQTLFVHCKSKDDDLGQHKLKSGTKYGFNFKENVWGTTLFWCSLRKPDNARVAFDVYWFDASTSHWLYTRCSWKNCIWIAKVDGIYIKNIPLNQDELVHTWK

Homology

[Close]

BLAST of Lag0002155 vs. NCBI nr
Match: XP_022152463.1 (S-protein homolog 1-like [Momordica charantia])

HSP 1 Score: 250.4 bits (638), Expect = 9.7e-63
Identity = 107/146 (73.29%), Postives = 120/146 (82.19%), Query Frame = 0


Query: 1   MGVLKNPLSFITIVGLVLVGSSSVALGGWPLPKWRVDIVNELSNGQTLFVHCKSKDDDLG 60
           MG +K PL F+ +V LVL+G S+VAL  WP+P W VDIVNELSNGQ LFVHCKSKDDDLG
Sbjct: 1   MGGIKKPLGFVIVVALVLLGCSTVALATWPMPTWTVDIVNELSNGQELFVHCKSKDDDLG 60

Query: 61  QHKLKSGTKYGFNFKENVWGTTLFWCSLRKPDNARVAFDVYWFDASTSHWLYTRCSWKNC 120
           +H L SG +YGF FK+NVW TTLFWC LRKPDN+  AFDVYW+D S  HWLYTRC +KNC
Sbjct: 61  EHNLDSGAQYGFTFKDNVWQTTLFWCYLRKPDNSHAAFDVYWYDISKGHWLYTRCDYKNC 120

Query: 121 IWIAKVDGIYIKNIPLNQDELVHTWK 147
           IWIAK DGIYIKNIP NQDELVH W+
Sbjct: 121 IWIAKADGIYIKNIPSNQDELVHPWE 146

[Close]

BLAST of Lag0002155 vs. NCBI nr
Match: XP_022143694.1 (S-protein homolog 1-like [Momordica charantia])

HSP 1 Score: 219.2 bits (557), Expect = 2.4e-53
Identity = 92/147 (62.59%), Postives = 115/147 (78.23%), Query Frame = 0


Query: 1   MGVLKNPLSFITIVGLVLVGSSSVALGGWP-LPKWRVDIVNELSNGQTLFVHCKSKDDDL 60
           MG LK PL F+ +V LVL+  S++A   WP  P W V+IVNEL++ QTLFVHC+SKDDDL
Sbjct: 1   MGCLKEPLGFMIVVALVLLHCSTLASSAWPSWPTWHVNIVNELNSEQTLFVHCQSKDDDL 60

Query: 61  GQHKLKSGTKYGFNFKENVWGTTLFWCSLRKPDNARVAFDVYWFDASTSHWLYTRCSWKN 120
           G+H +  GT+Y F FK+N+WGTTLFWC LRKP+N+   FDVYW D    +WLYTRC+WK 
Sbjct: 61  GEHNISVGTQYSFAFKDNIWGTTLFWCYLRKPNNSHADFDVYWVDNGKGYWLYTRCNWKT 120

Query: 121 CIWIAKVDGIYIKNIPLNQDELVHTWK 147
           CIWIAK DGIYIK+IP+NQD+L+H W+
Sbjct: 121 CIWIAKDDGIYIKDIPVNQDQLIHKWE 147

[Close]

BLAST of Lag0002155 vs. NCBI nr
Match: XP_022143641.1 (S-protein homolog 1-like [Momordica charantia])

HSP 1 Score: 196.4 bits (498), Expect = 1.7e-46
Identity = 86/143 (60.14%), Postives = 106/143 (74.13%), Query Frame = 0


Query: 4   LKNPLSFITIVGLVLV-GSSSVALGGWPLPKWRVDIVNELSNGQTLFVHCKSKDDDLGQH 63
           +  P   + +V +VLV  S++ A   WP  KWRVDIVN LS+GQTLF+HCKSKDDDLG H
Sbjct: 1   MDGPKKLLILVAMVLVCSSTTTAHSTWP-NKWRVDIVNGLSSGQTLFIHCKSKDDDLGIH 60

Query: 64  KLKSGTKYGFNFKENVWGTTLFWCSLRKPDNARVAFDVYWFDASTSHWLYTRCSWKNCIW 123
            L  G K+G+NF++N+WGTTLFWC +RKPDNA  +F+VYW D S   WLY  C+W++CIW
Sbjct: 61  NLAVGAKFGWNFRDNLWGTTLFWCYMRKPDNANASFEVYWDDKSKEQWLYNFCNWEDCIW 120

Query: 124 IAKVDGIYIKNIPLNQDELVHTW 146
            AK DGIYIKNI  N+DEL H W
Sbjct: 121 TAKDDGIYIKNILGNKDELAHKW 142

[Close]

BLAST of Lag0002155 vs. NCBI nr
Match: XP_023005093.1 (S-protein homolog 1-like [Cucurbita maxima])

HSP 1 Score: 194.1 bits (492), Expect = 8.3e-46
Identity = 82/146 (56.16%), Postives = 100/146 (68.49%), Query Frame = 0


Query: 1   MGVLKNPLSFITIVGLVLVGSSSVALGGWPLPKWRVDIVNELSNGQTLFVHCKSKDDDLG 60
           MG L+  L  + +  L LV  S+VA+G WPL +W V +VN L  GQTL  HCKSK+DDLG
Sbjct: 1   MGDLRMSLRNMIVATLALVAFSTVAMGSWPLERWTVQVVNGLGGGQTLLAHCKSKNDDLG 60

Query: 61  QHKLKSGTKYGFNFKENVWGTTLFWCSLRKPDNARVAFDVYWFDASTSHWLYTRCSWKNC 120
            H L +G  + F FK+NVW TT FWC+L KP+NA  +FDVYW+D S   WLYTRC    C
Sbjct: 61  DHNLATGALFSFTFKDNVWQTTEFWCTLTKPNNAHASFDVYWYDTSKDQWLYTRCGDHTC 120

Query: 121 IWIAKVDGIYIKNIPLNQDELVHTWK 147
           +WI K DG+YIKN   NQDELVH W+
Sbjct: 121 VWIGKDDGVYIKNASTNQDELVHPWE 146

[Close]

BLAST of Lag0002155 vs. NCBI nr
Match: KAG6592857.1 (S-protein-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 193.0 bits (489), Expect = 1.8e-45
Identity = 81/146 (55.48%), Postives = 99/146 (67.81%), Query Frame = 0


Query: 1   MGVLKNPLSFITIVGLVLVGSSSVALGGWPLPKWRVDIVNELSNGQTLFVHCKSKDDDLG 60
           MG L+ PL  + +  L LV  S+VA+G WPL  W V +VN L  GQTL  HCKSK+DD+G
Sbjct: 1   MGDLRMPLRNMIVATLALVAFSTVAMGSWPLETWTVQVVNGLGGGQTLLAHCKSKNDDMG 60

Query: 61  QHKLKSGTKYGFNFKENVWGTTLFWCSLRKPDNARVAFDVYWFDASTSHWLYTRCSWKNC 120
              + +G  Y F FK+NVW TT FWC+L KP+NA  +FDVYW+D S   WLYTRC    C
Sbjct: 61  DQNIATGALYSFTFKDNVWQTTEFWCTLTKPNNAHASFDVYWYDTSKDQWLYTRCGDHTC 120

Query: 121 IWIAKVDGIYIKNIPLNQDELVHTWK 147
           +WI K DG+YIKN   NQDELVH W+
Sbjct: 121 VWIGKDDGVYIKNASTNQDELVHPWE 146

[Close]

BLAST of Lag0002155 vs. ExPASy Swiss-Prot
Match: F4JLS0 (S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.4e-27
Identity = 52/116 (44.83%), Postives = 75/116 (64.66%), Query Frame = 0


Query: 31  LPKWRVDIVNELSNGQTLFVHCKSKDDDLGQHKLKSGTKYGFNFKENVWGTTLFWCSLRK 90
           + +W+V +VN L+ G+TLF+HCKSK+DDLG+  LK   ++ +NF EN+  +T FWC + K
Sbjct: 38  ISEWQVTVVNGLTTGETLFIHCKSKEDDLGEINLKFRNRFSWNFGENMLHSTFFWCYMNK 97

Query: 91  PDNARVAFDVYWFDASTSHWLYTRCSWKNCIWIAKVDGIYIKNIPLNQDELVHTWK 147
            DN  +  +V+W D      L+ RC WKNCIW AK DG+Y+ N    +D L   W+
Sbjct: 98  -DNGHMNVNVFWDDVI----LFHRCGWKNCIWTAKTDGLYLWNSASGEDVLSRKWE 148

[Close]

BLAST of Lag0002155 vs. ExPASy Swiss-Prot
Match: Q2HQ46 (S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 9.9e-26
Identity = 50/116 (43.10%), Postives = 74/116 (63.79%), Query Frame = 0


Query: 31  LPKWRVDIVNELSNGQTLFVHCKSKDDDLGQHKLKSGTKYGFNFKENVWGTTLFWCSLRK 90
           + +W+V + N L+ G+TLF+HCKSK++DLG   LK   ++ +NF EN+  +TLFWC + K
Sbjct: 38  ISEWQVTVANGLTTGETLFIHCKSKENDLGDINLKFLDRFSWNFGENMLHSTLFWCYMSK 97

Query: 91  PDNARVAFDVYWFDASTSHWLYTRCSWKNCIWIAKVDGIYIKNIPLNQDELVHTWK 147
            D+  +   V+W D      L+ RC WKNC+W AK DG+Y+ N  + +D L   WK
Sbjct: 98  -DDGHMNVKVFWDDVI----LFHRCDWKNCVWTAKNDGLYLWNSAIGEDVLSEKWK 148

[Close]

BLAST of Lag0002155 vs. ExPASy Swiss-Prot
Match: F4JLQ5 (S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.1e-14
Identity = 40/117 (34.19%), Postives = 60/117 (51.28%), Query Frame = 0


Query: 29  WPLPKWRVDIVNELSNGQTLFVHCKSKDDDLGQHKLKSGTKYGFNFKENVWGTTLFWCSL 88
           +P  K  V+I N+L N  TL  HCKSKDDDLG   L+ G  + F+F    +G TL++CS 
Sbjct: 43  FPTSKRTVEINNDLGNQLTLLYHCKSKDDDLGNRTLQPGESWSFSFGRQFFGRTLYFCSF 102

Query: 89  RKPDNARVAFDVYWFDASTSHWLYTRCSWKNCIWIAKVDGIYIKNIPLNQDELVHTW 146
             P+ +  +FD+Y     +      +C    C+W  + +G    N    Q +L + W
Sbjct: 103 SWPNESH-SFDIYKDHRDSGG--DNKCESDRCVWKIRRNGPCRFNDETKQFDLCYPW 156

[Close]

BLAST of Lag0002155 vs. ExPASy Swiss-Prot
Match: F2Q9V4 (S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 9.6e-13
Identity = 50/139 (35.97%), Postives = 71/139 (51.08%), Query Frame = 0


Query: 11  ITIVGLVLVGSSSVALGG--WPL--PKWRVDIVNELSNGQTLFVHCKSKDDDLGQHKLKS 70
           I IV + L+G  ++   G  +P+  P  RV I N+  N   L VHCKS+DDD G H L+ 
Sbjct: 5   IFIVLISLIGCETLQHDGKVFPMKGPLTRVVIYND--NDYLLGVHCKSRDDDHGFHILQK 64

Query: 71  GTKYGFNFKENVWGTTLFWCSLRKPDNARVAFDVYWFDASTSHWLYTRCSWKNCIWIAKV 130
           G  YG+ F  N   +TL++C   +    +  FD+Y     +S     RC  +NC W AK 
Sbjct: 65  GGLYGWMFYVNFMNSTLYFCGFSQEQVKKGVFDIYKAVRDSS-----RC--RNCTWEAKE 124

Query: 131 DGIYIKNIPLNQDELVHTW 146
           DGIY       ++ L + W
Sbjct: 125 DGIYGYGEIPKKNPLFYKW 134

[Close]

BLAST of Lag0002155 vs. ExPASy Swiss-Prot
Match: Q9LW22 (S-protein homolog 21 OS=Arabidopsis thaliana OX=3702 GN=SPH21 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 6.2e-12
Identity = 51/137 (37.23%), Postives = 69/137 (50.36%), Query Frame = 0


Query: 4   LKNPLSFITIVGLVLVGSSSVALGGWPLPKWRVDIVNELS--NGQTLFVHCKSKDDDLGQ 63
           +KN   F+ +VGL ++   S   G     K  + + NEL+  N   L VHCKSK++D+G 
Sbjct: 1   MKNLSIFLFVVGLCMI---SDVYG----KKSTITVKNELNPKNKNILKVHCKSKNNDIGV 60

Query: 64  HKLKSGTKYGFNFKENVWGTTLFWCSLRK-PDNARV-------AFDVYWFDASTSHWLYT 123
             LK G    F+FK N WGTT FWC+L K PD  R        A  ++  D S+ +WL  
Sbjct: 61  KYLKIGEVMSFSFKTNFWGTTEFWCNLYKGPDYKRYRGITAYQAIGLFAKDGSSYNWL-- 118

Query: 124 RCSWKNCIWIAKVDGIY 131
                     A+ DGIY
Sbjct: 121 ----------ARDDGIY 118

The following BLAST results are available for this feature:

BLAST of Lag0002155 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152463.1	9.7e-63	73.29	S-protein homolog 1-like [Momordica charantia]	[more]
XP_022143694.1	2.4e-53	62.59	S-protein homolog 1-like [Momordica charantia]	[more]
XP_022143641.1	1.7e-46	60.14	S-protein homolog 1-like [Momordica charantia]	[more]
XP_023005093.1	8.3e-46	56.16	S-protein homolog 1-like [Cucurbita maxima]	[more]
KAG6592857.1	1.8e-45	55.48	S-protein-like 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0002155 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JLS0	2.4e-27	44.83	S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1	[more]
Q2HQ46	9.9e-26	43.10	S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1	[more]
F4JLQ5	5.1e-14	34.19	S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1	[more]
F2Q9V4	9.6e-13	35.97	S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2	[more]
Q9LW22	6.2e-12	37.23	S-protein homolog 21 OS=Arabidopsis thaliana OX=3702 GN=SPH21 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010264	Plant self-incompatibility S1	PFAM	PF05938	Self-incomp_S1	coord: 36..145 e-value: 2.6E-28 score: 98.6
IPR010264	Plant self-incompatibility S1	PANTHER	PTHR31232	FAMILY NOT NAMED	coord: 31..137
None	No IPR available	PANTHER	PTHR31232:SF34	PUTATIVE-RELATED	coord: 31..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002155.1	Lag0002155.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0060320	rejection of self pollen
cellular_component	GO:0005576	extracellular region