Lag0002152 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002152
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DEAD-box ATP-dependent RNA helicase 31-like
Location	chr4: 39906315 .. 39906903 (+)
RNA-Seq Expression	Lag0002152
Synteny	Lag0002152

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAATGAAGGTCCTTGTTCTTGACGAAGCTGATCATTTATTAGACATGGGATTTCGAAAGGACATCGAGAGGATTATTGATGCAGTTCCAAAGCAAAGGCAAACTCTTTTATTTTCAACCACGGTTCCAGAAGAGGTATTTATTTACTTGCTGCTTTTTCCGTCCTGTTTTTCATACTTCTTCATTTTAAATATAAGCAAATATAAAATGCTAAACATTCCTCTTGAATAAAGGTTCGCCAAATCAGTCACAGTGCTTTGAGAAGAGATCATGAATCTGTCAATACAATTCATGAACAGGTAGGACTTATCATAAGCAGCTGTATCTTCTTCTGGCTCCTTTACCATCTTTAATGAATTTGGTCATCTAATAACAAGCTGGATCATTTCAGGTGAGACAAATGCAAATAGTTGCTCCATTGGAGAAGCATTTTAGTCTACTCTATGCTATACTTAAAGAACATATTGCTGATGACGTTGACTATAAGGTATCGACTTTAATCAGGGATAACAAATTTTTACCAATAGTTCAGTTTTAGTAATATCCTTATCTGTTATAATGAAGATAGGAAGTTCCTAGCTATTAG

mRNA sequence

ATGGGAATGAAGGTCCTTGTTCTTGACGAAGCTGATCATTTATTAGACATGGGATTTCGAAAGGACATCGAGAGGATTATTGATGCAGTTCCAAAGCAAAGGCAAACTCTTTTATTTTCAACCACGGTTCCAGAAGAGGTTCGCCAAATCAGTCACAGTGCTTTGAGAAGAGATCATGAATCTGTCAATACAATTCATGAACAGGTGAGACAAATGCAAATAGTTGCTCCATTGGAGAAGCATTTTAGTCTACTCTATGCTATACTTAAAGAACATATTGCTGATGACGTTGACTATAAGGAAGTTCCTAGCTATTAG

Coding sequence (CDS)

ATGGGAATGAAGGTCCTTGTTCTTGACGAAGCTGATCATTTATTAGACATGGGATTTCGAAAGGACATCGAGAGGATTATTGATGCAGTTCCAAAGCAAAGGCAAACTCTTTTATTTTCAACCACGGTTCCAGAAGAGGTTCGCCAAATCAGTCACAGTGCTTTGAGAAGAGATCATGAATCTGTCAATACAATTCATGAACAGGTGAGACAAATGCAAATAGTTGCTCCATTGGAGAAGCATTTTAGTCTACTCTATGCTATACTTAAAGAACATATTGCTGATGACGTTGACTATAAGGAAGTTCCTAGCTATTAG

Protein sequence

MGMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHESVNTIHEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYKEVPSY

Homology

[Close]

BLAST of Lag0002152 vs. NCBI nr
Match: XP_038898727.1 (DEAD-box ATP-dependent RNA helicase 31-like [Benincasa hispida])

HSP 1 Score: 174.9 bits (442), Expect = 3.7e-40
Identity = 92/107 (85.98%), Postives = 95/107 (88.79%), Query Frame = 0


Query: 1   MGMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHE 60
           MG+KVLVLDEADHLLDMGFRKDIERI+ AVPKQRQTLLFS TVP+EVRQISH ALRRDHE
Sbjct: 468 MGVKVLVLDEADHLLDMGFRKDIERIVAAVPKQRQTLLFSATVPDEVRQISHIALRRDHE 527

Query: 61  SVNTI-------HEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYK 101
            VNTI       H QVRQMQIVAPLEKHFSLLYAILKEHIADDVDYK
Sbjct: 528 FVNTIQEGSEDTHAQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYK 574

[Close]

BLAST of Lag0002152 vs. NCBI nr
Match: KAA0050997.1 (DEAD-box ATP-dependent RNA helicase 31-like [Cucumis melo var. makuwa] >TYK04387.1 DEAD-box ATP-dependent RNA helicase 31-like [Cucumis melo var. makuwa])

HSP 1 Score: 173.3 bits (438), Expect = 1.1e-39
Identity = 92/107 (85.98%), Postives = 94/107 (87.85%), Query Frame = 0


Query: 1   MGMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHE 60
           MG+KVLVLDEADHLLDMGFRKDIERII AVPKQRQTLLFS TVP+EVRQISH ALRRDHE
Sbjct: 426 MGVKVLVLDEADHLLDMGFRKDIERIIAAVPKQRQTLLFSATVPDEVRQISHIALRRDHE 485

Query: 61  SVNTI-------HEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYK 101
            VNTI       H QVRQM IVAPLEKHFSLLYAILKEHIADDVDYK
Sbjct: 486 FVNTIQEGSEDTHAQVRQMHIVAPLEKHFSLLYAILKEHIADDVDYK 532

[Close]

BLAST of Lag0002152 vs. NCBI nr
Match: XP_011659146.2 (DEAD-box ATP-dependent RNA helicase 31 [Cucumis sativus] >XP_011659147.2 DEAD-box ATP-dependent RNA helicase 31 [Cucumis sativus] >XP_011659148.2 DEAD-box ATP-dependent RNA helicase 31 [Cucumis sativus] >KAE8646212.1 hypothetical protein Csa_015831 [Cucumis sativus])

HSP 1 Score: 173.3 bits (438), Expect = 1.1e-39
Identity = 92/107 (85.98%), Postives = 95/107 (88.79%), Query Frame = 0


Query: 1   MGMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHE 60
           MG+KVLVLDEADHLLDMGFRKDIERII AVPKQRQTLLFS TVP+EVRQISH ALRRDHE
Sbjct: 510 MGVKVLVLDEADHLLDMGFRKDIERIIAAVPKQRQTLLFSATVPDEVRQISHIALRRDHE 569

Query: 61  SVNTI-------HEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYK 101
            VNTI       H QVRQMQIVAPLEKHFSLLYAILKEHIA+DVDYK
Sbjct: 570 FVNTIEEGSEDTHAQVRQMQIVAPLEKHFSLLYAILKEHIANDVDYK 616

[Close]

BLAST of Lag0002152 vs. NCBI nr
Match: XP_008461517.1 (PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Cucumis melo] >XP_008461520.1 PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Cucumis melo])

HSP 1 Score: 173.3 bits (438), Expect = 1.1e-39
Identity = 92/107 (85.98%), Postives = 94/107 (87.85%), Query Frame = 0


Query: 1   MGMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHE 60
           MG+KVLVLDEADHLLDMGFRKDIERII AVPKQRQTLLFS TVP+EVRQISH ALRRDHE
Sbjct: 477 MGVKVLVLDEADHLLDMGFRKDIERIIAAVPKQRQTLLFSATVPDEVRQISHIALRRDHE 536

Query: 61  SVNTI-------HEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYK 101
            VNTI       H QVRQM IVAPLEKHFSLLYAILKEHIADDVDYK
Sbjct: 537 FVNTIQEGSEDTHAQVRQMHIVAPLEKHFSLLYAILKEHIADDVDYK 583

[Close]

BLAST of Lag0002152 vs. NCBI nr
Match: XP_022158751.1 (DEAD-box ATP-dependent RNA helicase 31-like [Momordica charantia])

HSP 1 Score: 172.6 bits (436), Expect = 1.9e-39
Identity = 92/107 (85.98%), Postives = 93/107 (86.92%), Query Frame = 0


Query: 1   MGMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHE 60
           MG+KVLVLDEADHLLDMGFRKDIERII AVPKQRQTLLFS TVPEEVRQI H ALRRDHE
Sbjct: 452 MGVKVLVLDEADHLLDMGFRKDIERIIAAVPKQRQTLLFSATVPEEVRQICHIALRRDHE 511

Query: 61  SVNTI-------HEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYK 101
            VNTI       H QVRQM IVAPLEKHFSLLYAILKEHIADDVDYK
Sbjct: 512 FVNTIEEGSEETHAQVRQMHIVAPLEKHFSLLYAILKEHIADDVDYK 558

[Close]

BLAST of Lag0002152 vs. ExPASy Swiss-Prot
Match: Q9FNM7 (DEAD-box ATP-dependent RNA helicase 26 OS=Arabidopsis thaliana OX=3702 GN=RH26 PE=1 SV=2)

HSP 1 Score: 149.8 bits (377), Expect = 1.7e-35
Identity = 75/109 (68.81%), Postives = 90/109 (82.57%), Query Frame = 0


Query: 1   MGMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHE 60
           MG+KVLVLDEADHLLDMGFR+DIERII AVPKQRQT LFS TVPEEVRQI H AL+RDHE
Sbjct: 536 MGVKVLVLDEADHLLDMGFRRDIERIIAAVPKQRQTFLFSATVPEEVRQICHVALKRDHE 595

Query: 61  SVNTI-------HEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYKEV 103
            +N +       H++V QM ++A L++HFSLL+ +LKEHIAD+VDYK +
Sbjct: 596 FINCVQEGSGETHQKVTQMYMIASLDRHFSLLHVLLKEHIADNVDYKVI 644

[Close]

BLAST of Lag0002152 vs. ExPASy Swiss-Prot
Match: Q9FFQ1 (DEAD-box ATP-dependent RNA helicase 31 OS=Arabidopsis thaliana OX=3702 GN=RH31 PE=2 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 8.4e-35
Identity = 76/108 (70.37%), Postives = 87/108 (80.56%), Query Frame = 0


Query: 2   GMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHES 61
           G+KVLVLDEADHLLDMGFRKDIERII AVPK+RQT LFS TVPEEVRQI   ALRRDHE 
Sbjct: 403 GVKVLVLDEADHLLDMGFRKDIERIISAVPKERQTFLFSATVPEEVRQICLVALRRDHEF 462

Query: 62  VNTIHE-------QVRQMQIVAPLEKHFSLLYAILKEHIADDVDYKEV 103
           VN +HE       QVRQM ++A L++HFSLLY +L+EHI  +VDYK +
Sbjct: 463 VNCVHEGTIETHQQVRQMHMIASLDRHFSLLYTLLREHIMGNVDYKVI 510

[Close]

BLAST of Lag0002152 vs. ExPASy Swiss-Prot
Match: Q0DBU5 (DEAD-box ATP-dependent RNA helicase 31 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0526600 PE=3 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 7.8e-33
Identity = 69/109 (63.30%), Postives = 88/109 (80.73%), Query Frame = 0


Query: 1   MGMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHE 60
           MG+KVL+LDEAD LLDMGFR DIERI+ A+PKQRQTLLFS TVP+EVRQ+ H A++RD E
Sbjct: 233 MGVKVLILDEADRLLDMGFRTDIERIVAALPKQRQTLLFSATVPDEVRQVCHIAMKRDLE 292

Query: 61  SVNTI-------HEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYKEV 103
            VNT+       H QV+QM +VAPL+K FS+LY +L +HI+++VDYK +
Sbjct: 293 FVNTVEEGSEETHSQVKQMHVVAPLDKQFSILYGLLTDHISENVDYKVI 341

[Close]

BLAST of Lag0002152 vs. ExPASy Swiss-Prot
Match: Q94C75 (DEAD-box ATP-dependent RNA helicase 25 OS=Arabidopsis thaliana OX=3702 GN=RH25 PE=2 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 1.0e-32
Identity = 71/109 (65.14%), Postives = 87/109 (79.82%), Query Frame = 0


Query: 1   MGMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHE 60
           MG+KVLVLDEADHLLDMGFR++IERII AVPKQRQT LFS TV +EVRQI H AL+RDHE
Sbjct: 234 MGVKVLVLDEADHLLDMGFRREIERIIAAVPKQRQTFLFSATVSDEVRQICHVALKRDHE 293

Query: 61  SVNTI-------HEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYKEV 103
            VN +       H++V QM ++A L++HFSLLY +LK+HI D+V YK +
Sbjct: 294 FVNCVQEGAGETHQKVSQMYMIASLDRHFSLLYGLLKKHITDNVGYKVI 342

[Close]

BLAST of Lag0002152 vs. ExPASy Swiss-Prot
Match: Q5ZBH5 (DEAD-box ATP-dependent RNA helicase 25 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0618400 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 2.3e-32
Identity = 68/108 (62.96%), Postives = 89/108 (82.41%), Query Frame = 0


Query: 2   GMKVLVLDEADHLLDMGFRKDIERIIDAVPKQRQTLLFSTTVPEEVRQISHSALRRDHES 61
           G+KVLVLDEAD LLDMGFR+DIERII +VPK+RQTLLFS TVPEEVRQISH A++++++ 
Sbjct: 281 GVKVLVLDEADRLLDMGFRRDIERIIASVPKERQTLLFSATVPEEVRQISHIAMKKNYKF 340

Query: 62  VNTI-------HEQVRQMQIVAPLEKHFSLLYAILKEHIADDVDYKEV 103
           +NT+       H QV QM ++APL+ HFS+LY +LK+H+A+D DYK +
Sbjct: 341 INTVKDGDEETHAQVSQMFMIAPLDLHFSILYDVLKKHVAEDADYKVI 388

The following BLAST results are available for this feature:

BLAST of Lag0002152 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898727.1	3.7e-40	85.98	DEAD-box ATP-dependent RNA helicase 31-like [Benincasa hispida]	[more]
KAA0050997.1	1.1e-39	85.98	DEAD-box ATP-dependent RNA helicase 31-like [Cucumis melo var. makuwa] >TYK04387...	[more]
XP_011659146.2	1.1e-39	85.98	DEAD-box ATP-dependent RNA helicase 31 [Cucumis sativus] >XP_011659147.2 DEAD-bo...	[more]
XP_008461517.1	1.1e-39	85.98	PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Cucumis melo] >XP_008461...	[more]
XP_022158751.1	1.9e-39	85.98	DEAD-box ATP-dependent RNA helicase 31-like [Momordica charantia]	[more]

BLAST of Lag0002152 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FNM7	1.7e-35	68.81	DEAD-box ATP-dependent RNA helicase 26 OS=Arabidopsis thaliana OX=3702 GN=RH26 P...	[more]
Q9FFQ1	8.4e-35	70.37	DEAD-box ATP-dependent RNA helicase 31 OS=Arabidopsis thaliana OX=3702 GN=RH31 P...	[more]
Q0DBU5	7.8e-33	63.30	DEAD-box ATP-dependent RNA helicase 31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q94C75	1.0e-32	65.14	DEAD-box ATP-dependent RNA helicase 25 OS=Arabidopsis thaliana OX=3702 GN=RH25 P...	[more]
Q5ZBH5	2.3e-32	62.96	DEAD-box ATP-dependent RNA helicase 25 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011545	DEAD/DEAH box helicase domain	PFAM	PF00270	DEAD	coord: 3..49 e-value: 1.3E-10 score: 41.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 2..75 e-value: 6.9E-18 score: 66.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 3..56
None	No IPR available	PANTHER	PTHR24031:SF688	DEAD-BOX ATP-DEPENDENT RNA HELICASE 25	coord: 1..96
None	No IPR available	PANTHER	PTHR24031	RNA HELICASE	coord: 1..96
IPR014001	Helicase superfamily 1/2, ATP-binding domain	PROSITE	PS51192	HELICASE_ATP_BIND_1	coord: 1..61 score: 12.074646

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002152.1	Lag0002152.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003724	RNA helicase activity