Lag0002135 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002135 vs. NCBI nr
Match: XP_038885921.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 298.5 bits (763), Expect = 7.5e-77
Identity = 159/316 (50.32%), Postives = 212/316 (67.09%), Query Frame = 0

Query: 29  ENLSLADWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAPLVREEFALAKLR 88
           E +S+++W K  VL   S+ G VYLG+P KP+LA VAIKSAP+  +  L+RE+  L K  
Sbjct: 8   EKISISNWDKGSVLGRGSF-GHVYLGVPNKPYLANVAIKSAPYQTACSLIREKVVLQKFI 67

Query: 89  PYPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTIL 148
             P IV+ IG            +Y+L+LE+  +G+LA LI+ R   L   + + YLR IL
Sbjct: 68  GCPEIVQYIGEALTVNTDDNI-VYDLILEYAASGSLAHLIKTRGRLLEREV-KVYLRMIL 127

Query: 149 QGLSSVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKRRL 208
           QGLS +HA GYVHCDLKP+NIL FP  D +   LKI++F L+  Y      +      +L
Sbjct: 128 QGLSCIHARGYVHCDLKPENILVFPIGDRKKMKLKIADFRLSERYFGKKLEESG----KL 187

Query: 209 RFRGTPRYMSPESLTGEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLLDETA 268
           +FRGTP+YMSPESL G+IKP+LD+WS+GCV V+MI+G K +WN C+TR+EVL KL+ E  
Sbjct: 188 KFRGTPKYMSPESLIGKIKPSLDIWSLGCVLVNMISG-KCVWNDCTTREEVLLKLMFEMK 247

Query: 269 TPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYLLERDEEEQILDETRCSQRLPF 328
           TP++PEELS  GK FL+KCFERNPK RW+ DMLL+HPYL+E  EE+ I       + LP 
Sbjct: 248 TPNIPEELSFQGKDFLQKCFERNPKERWSADMLLNHPYLVE--EEDSI-------EMLPI 305

Query: 329 FVGRLCSASIVVVVLF 345
           F+G L +  I+V +LF
Sbjct: 308 FIG-LLTIMIIVAILF 305

BLAST of Lag0002135 vs. NCBI nr
Match: XP_022131647.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-62
Identity = 132/285 (46.32%), Postives = 186/285 (65.26%), Query Frame = 0

Query: 29  ENLSLADWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAP-FNDSAPLVREEFALAKL 88
           +++S+ DW KA+ L   SY G V+LG PTKP L  VAIK AP +  ++ L++E+  L + 
Sbjct: 11  DDISVFDWNKAQFLGKGSY-GKVFLGTPTKPDLGFVAIKVAPLWQKTSSLIKEKVVLEQF 70

Query: 89  RPYPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTI 148
              P IV+ IG     +      +YNL+LE+   GTLAD+I+ +   L    A++YLR I
Sbjct: 71  IGCPEIVQYIGDAVTIKRDDHL-VYNLILEYAAGGTLADIIK-KQKKLQEEDAKQYLRMI 130

Query: 149 LQGLSSVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKRR 208
           L+GLS +HA  +VH DLKP NILAFP  DG    LKI++F LAR Y      D      +
Sbjct: 131 LRGLSCIHAKSFVHADLKPTNILAFPLKDGR-MQLKIADFGLARIYYKKEERDFYWKNNK 190

Query: 209 LRFRGTPRYMSPESLTGEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLLDET 268
           ++F GT  YMSPES+ G I P+LD+WS+GC+F++MI G + +WN C T +++++KL+ E 
Sbjct: 191 MKFVGTTEYMSPESVVGNIDPSLDIWSLGCIFLNMITG-ECVWNDCLTYEQLITKLVYEG 250

Query: 269 ATPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYLLERDE 313
            TP +PEELS  GK FL+KCFERN + RW+VD L  HPYL++ ++
Sbjct: 251 ETPTIPEELSDLGKDFLQKCFERNYEERWSVDRLFRHPYLVQENK 290

BLAST of Lag0002135 vs. NCBI nr
Match: XP_022131646.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 227.3 bits (578), Expect = 2.1e-55
Identity = 128/283 (45.23%), Postives = 175/283 (61.84%), Query Frame = 0

Query: 25  SFHGENLSLADWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAPLVREEFAL 84
           SF   +LS  DW K  VL   S+ G V+LG   +  L +VAIK AP   ++ LV+E+  L
Sbjct: 6   SFKEYDLSAFDWDKTPVLGKGSH-GKVFLG---ELDLGSVAIKVAPCRAASSLVKEKAIL 65

Query: 85  AKLRPYPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYL 144
            +      IV+ IG T   +      +Y+L+LE+   GTL D+I+ +   +    A++YL
Sbjct: 66  KQFIGCSKIVQYIGDTVTKKGDD--VVYSLILEYATGGTLGDIIR-KQEKISEEDAKKYL 125

Query: 145 RTILQGLSSVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAP 204
           R ILQGLS +HA G+VH DLK DNILAF S  G    LKI++F L+R Y           
Sbjct: 126 RMILQGLSCIHAKGFVHADLKSDNILAFRSKSGR-MKLKIADFGLSRMYDEKEEKSLCWN 185

Query: 205 KRRLRFRGTPRYMSPESLTGEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLL 264
             ++ F GT +YMSPES+   +KPALD+WS+GC+FV+M+ G + +WN C   KE++SKL+
Sbjct: 186 YYKMSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTG-RHVWNDCKDNKELISKLI 245

Query: 265 DETATPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYL 308
            E   P +P+ELS  GK FL KCFERN ++RW+ DMLL HPYL
Sbjct: 246 YEREIPTIPKELSELGKDFLEKCFERNYEKRWSADMLLRHPYL 279

BLAST of Lag0002135 vs. NCBI nr
Match: XP_022150204.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 224.6 bits (571), Expect = 1.4e-54
Identity = 128/296 (43.24%), Postives = 175/296 (59.12%), Query Frame = 0

Query: 33  LADWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAPLVREEFALAKLRPYPH 92
           + +W +   L   SY G V+L  PT   L   A+K A     + L+ E+  L     +P 
Sbjct: 1   MTNWVRVXDLGKGSY-GKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPE 60

Query: 93  IVRSIGYTHHTRAQS-RFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTILQGL 152
           IV+  G    T   S     Y+LVLE+   GTLADLI+ +   L  +  + YL+ IL+GL
Sbjct: 61  IVQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIR-KQKKLSENTVKEYLKMILRGL 120

Query: 153 SSVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKRRLRFR 212
           S +H  G+VH DLKP NILAFP  DG+   LKI++F L+R  R   + + +    +L+FR
Sbjct: 121 SCIHKLGFVHVDLKPANILAFPQIDGK-MKLKIADFGLSR--RCNKKRECNF---KLKFR 180

Query: 213 GTPRYMSPESLTGEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLLDETATPD 272
           GTP YMSPES+ GE+   LD+WS+GC+ V+M+ G K  W+ C   ++++ KL     TP 
Sbjct: 181 GTPMYMSPESIYGEVNTPLDIWSLGCILVEMVTG-KHAWDDCIDLEQLMLKLRVNMETPK 240

Query: 273 VPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYLLERDEEEQILDETRCSQRLP 328
           +PEELS  GK FL KCF+RNPKRRWT DMLL HPY LE+++E    D+      LP
Sbjct: 241 IPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPY-LEQEKEAIKKDDDPTYSYLP 286

BLAST of Lag0002135 vs. NCBI nr
Match: XP_022154080.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 214.9 bits (546), Expect = 1.1e-51
Identity = 125/278 (44.96%), Postives = 169/278 (60.79%), Query Frame = 0

Query: 36  WKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAPLVREEFALAKLRPYPHIVR 95
           W K + L   SY G V+L   T   +  VA+K AP   S+ L  E+  L  LR    IV+
Sbjct: 4   WTKVKELGRGSY-GKVFLAKSTIGPIDYVAVKVAPVERSSSLELEKDILQHLRGCSEIVQ 63

Query: 96  SIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTILQGLSSVH 155
            IG      ++     Y L+LE+   GTLA+LI+ +   L  S  + YLR IL+GLS +H
Sbjct: 64  CIG------SEMTSVCYYLILEYAGGGTLAELIR-KQKKLTESKVKEYLRMILRGLSCIH 123

Query: 156 ATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKRRLRFRGTPR 215
             G+VH DLKP NILAFP  +G+   LKI++F L++  R     D+ A     RFRGT R
Sbjct: 124 RRGFVHADLKPANILAFPRPNGK-MKLKIADFGLSK--RWVVGQDEAAEHSEPRFRGTAR 183

Query: 216 YMSPESLT-GEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLLDETATP-DVP 275
           YMSPES+  G+I P LD+WS+GC+F++MI G+  +WN C T  ++L KL++ET  P DV 
Sbjct: 184 YMSPESIIFGKISPPLDIWSLGCIFIEMITGSP-VWNSCRTYDQLLKKLVNETELPTDVL 243

Query: 276 EELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYLLERD 312
           ++LS  G+ FL  C +R+ KRRWT DMLL HPYL E +
Sbjct: 244 KKLSKEGRDFLASCLDRDSKRRWTADMLLKHPYLSEEN 269

BLAST of Lag0002135 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.8e-34
Identity = 100/282 (35.46%), Postives = 149/282 (52.84%), Query Frame = 0

Query: 62  ATVAIKSAPFNDSAPLVREEFALAKLRPYPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPT 121
           A +A+KS     +A L  E+  L  L   P I+R   Y   +  ++   ++NL+LE+   
Sbjct: 31  ALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRC--YGEDSTVENGEEMHNLLLEYASR 90

Query: 122 GTLADLI-QARTNNLPHSLARRYLRTILQGLSSVHATGYVHCDLKPDNILAFPSADGESF 181
           G+LA  + +     LP S  RR+  ++L+GL  +HA G+ HCD+K  NIL F   DG   
Sbjct: 91  GSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKGFAHCDIKLANILLF--NDG--- 150

Query: 182 DLKISEFSLARWYRSTWRSDDD--APKRRLRFRGTPRYMSPESLT-GEIKPALDVWSVGC 241
            +KI++F LA       R D D  A ++ +  RGTP YM+PE +   E   A DVW++GC
Sbjct: 151 SVKIADFGLA------MRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGC 210

Query: 242 VFVDMINGNKGIWN--GCSTRKEVLSKLLDETATPDVPEELSAGGKYFLRKCFERNPKRR 301
             V+M +G K  W+    S    +L ++      P +PE LS  GK FL KCF ++P +R
Sbjct: 211 AVVEMFSG-KTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKR 270

Query: 302 WTVDMLLDHPYL---LERDEEEQIL------DETRCSQRLPF 329
           WT +MLL+H ++   LE D  E  +      D+   S + PF
Sbjct: 271 WTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPF 298

BLAST of Lag0002135 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.1e-29
Identity = 88/289 (30.45%), Postives = 141/289 (48.79%), Query Frame = 0

Query: 35  DWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAPLVREEFALAKLRPYPHIV 94
           +W + R+L   S    VY           +A+KS+  + S  L RE   L+ L   P++ 
Sbjct: 2   EWTRGRILGRGS-TATVYAAAGHNSD-EILAVKSSEVHRSEFLQREAKILSSLSS-PYV- 61

Query: 95  RSIGYTHHTRAQSRFGI--YNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTILQGLS 154
             IGY      +   G+  YNL++E+ P GTL D        +  +   +Y R IL+GL 
Sbjct: 62  --IGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLE 121

Query: 155 SVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKRRLRFRG 214
            +H+ G VHCD+K  N++       E  + KI++F  A+     + S            G
Sbjct: 122 YIHSKGIVHCDVKGSNVVI-----SEKGEAKIADFGCAKRVDPVFESP---------VMG 181

Query: 215 TPRYMSPESLTGEIK-PALDVWSVGCVFVDMINGNKGIWNGCSTRKE---VLSKLLDETA 274
           TP +M+PE   GE +    D+W+VGC  ++M+ G+   W    +R++   VL ++   + 
Sbjct: 182 TPAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPP-WTKADSREDPVSVLYRVGYSSE 241

Query: 275 TPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYLLERDEEEQIL 318
           TP++P  L+   K FL KC +R    RWT   LL+HP+L  + + E +L
Sbjct: 242 TPELPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVL 269

BLAST of Lag0002135 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 6.1e-29
Identity = 84/259 (32.43%), Postives = 132/259 (50.97%), Query Frame = 0

Query: 63  TVAIKSAPFNDSAPLVREEFALAKLR-PYPHIVRSIGYTH---HTRAQSRFGIYNLVLEH 122
           T+A+KSA F+ S  L RE   L+ L  PY    R    T    H   ++    Y+L++E+
Sbjct: 28  TLAVKSAEFHRSEFLQREAKILSSLNSPYVIGYRGCEITREPFHNNGEAT--TYSLLMEY 87

Query: 123 TPTGTLADLIQARTNNLPHSLARRYLRTILQGLSSVH-ATGYVHCDLKPDNILAFPSADG 182
            P GTL D+       +  +   +Y R IL GL  +H + G  HCD+K  N+L      G
Sbjct: 88  APYGTLTDVATKNGGFIDEARVVKYTRQILLGLEYIHNSKGIAHCDIKGSNVLV-----G 147

Query: 183 ESFDLKISEFSLARWYRSTWRSDDDAPKRRLRFRGTPRYMSPESLTGEIK-PALDVWSVG 242
           E+ + KI++F  A+W           P+     RGTP +M+PE+  GE +    D+W+VG
Sbjct: 148 ENGEAKIADFGCAKWVE---------PEITEPVRGTPAFMAPEAARGERQGKESDIWAVG 207

Query: 243 CVFVDMINGNKGIWNGC--STRKEVLSKLLDETATPDVPEELSAGGKYFLRKCFERNPKR 302
           C  ++M+ G++  W G   +    VL ++      P++P  L+   K FL KC ++    
Sbjct: 208 CTVIEMVTGSQP-WIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFLGKCLKKEATE 267

Query: 303 RWTVDMLLDHPYLLERDEE 314
           RWT   LL+HP+L+ ++ E
Sbjct: 268 RWTASQLLNHPFLVNKEPE 269

BLAST of Lag0002135 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.9e-24
Identity = 82/289 (28.37%), Postives = 139/289 (48.10%), Query Frame = 0

Query: 34  ADWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAP-------LVREEFALAK 93
           + WKK +++   ++ G VY+   ++   A  A+K        P        + +E  L  
Sbjct: 344 SQWKKGKLIGRGTF-GSVYVASNSETG-ALCAMKEVELFPDDPKSAECIKQLEQEIKLLS 403

Query: 94  LRPYPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRT 153
              +P+IV+  G       + RF IY   LE+   G++   I+     +  S+ R + R 
Sbjct: 404 NLQHPNIVQYFG---SETVEDRFFIY---LEYVHPGSINKYIRDHCGTMTESVVRNFTRH 463

Query: 154 ILQGLSSVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKR 213
           IL GL+ +H    VH D+K  N+L   S       +K+++F +A+ + +  R+D      
Sbjct: 464 ILSGLAYLHNKKTVHRDIKGANLLVDASG-----VVKLADFGMAK-HLTGQRAD------ 523

Query: 214 RLRFRGTPRYMSPESLTG--------EIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKE 273
            L  +G+P +M+PE +          ++  A+D+WS+GC  ++M  G K  W+       
Sbjct: 524 -LSLKGSPYWMAPELMQAVMQKDSNPDLAFAVDIWSLGCTIIEMFTG-KPPWSEFEGAAA 583

Query: 274 VLSKLLDETATPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYL 308
           +   + D   +P +PE +S  GK FLR CF+RNP  R T  MLL+H +L
Sbjct: 584 MFKVMRD---SPPIPESMSPEGKDFLRLCFQRNPAERPTASMLLEHRFL 607

BLAST of Lag0002135 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 5.0e-23
Identity = 82/280 (29.29%), Postives = 132/280 (47.14%), Query Frame = 0

Query: 36  WKKARVLSTASYGGGVYLGIPTKP------HLATVAIKSAPFNDSAPLVREEFALAKLRP 95
           WKK R+L   S+ G VYLG  ++          T+        +SA  + +E ++     
Sbjct: 400 WKKGRLLGMGSF-GHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSRLR 459

Query: 96  YPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTILQ 155
           + +IV+   Y        +  IY   LE+   G++  L+Q        +  R Y + IL 
Sbjct: 460 HQNIVQ---YYGSETVDDKLYIY---LEYVSGGSIYKLLQ-EYGQFGENAIRNYTQQILS 519

Query: 156 GLSSVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKRRLR 215
           GL+ +HA   VH D+K  NIL  P        +K+++F +A+           A    L 
Sbjct: 520 GLAYLHAKNTVHRDIKGANILVDPHG-----RVKVADFGMAKHI--------TAQSGPLS 579

Query: 216 FRGTPRYMSPESL--TGEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLLDET 275
           F+G+P +M+PE +  +     A+D+WS+GC  ++M    K  W+        + K+ +  
Sbjct: 580 FKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMAT-TKPPWSQYE-GVPAMFKIGNSK 639

Query: 276 ATPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYL 308
             PD+P+ LS  GK F+RKC +RNP  R T   LLDH ++
Sbjct: 640 ELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

BLAST of Lag0002135 vs. ExPASy TrEMBL
Match: A0A6J1BRL7 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111004775 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 5.1e-63
Identity = 132/285 (46.32%), Postives = 186/285 (65.26%), Query Frame = 0

Query: 29  ENLSLADWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAP-FNDSAPLVREEFALAKL 88
           +++S+ DW KA+ L   SY G V+LG PTKP L  VAIK AP +  ++ L++E+  L + 
Sbjct: 11  DDISVFDWNKAQFLGKGSY-GKVFLGTPTKPDLGFVAIKVAPLWQKTSSLIKEKVVLEQF 70

Query: 89  RPYPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTI 148
              P IV+ IG     +      +YNL+LE+   GTLAD+I+ +   L    A++YLR I
Sbjct: 71  IGCPEIVQYIGDAVTIKRDDHL-VYNLILEYAAGGTLADIIK-KQKKLQEEDAKQYLRMI 130

Query: 149 LQGLSSVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKRR 208
           L+GLS +HA  +VH DLKP NILAFP  DG    LKI++F LAR Y      D      +
Sbjct: 131 LRGLSCIHAKSFVHADLKPTNILAFPLKDGR-MQLKIADFGLARIYYKKEERDFYWKNNK 190

Query: 209 LRFRGTPRYMSPESLTGEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLLDET 268
           ++F GT  YMSPES+ G I P+LD+WS+GC+F++MI G + +WN C T +++++KL+ E 
Sbjct: 191 MKFVGTTEYMSPESVVGNIDPSLDIWSLGCIFLNMITG-ECVWNDCLTYEQLITKLVYEG 250

Query: 269 ATPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYLLERDE 313
            TP +PEELS  GK FL+KCFERN + RW+VD L  HPYL++ ++
Sbjct: 251 ETPTIPEELSDLGKDFLQKCFERNYEERWSVDRLFRHPYLVQENK 290

BLAST of Lag0002135 vs. ExPASy TrEMBL
Match: A0A6J1BQ31 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111004774 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.0e-55
Identity = 128/283 (45.23%), Postives = 175/283 (61.84%), Query Frame = 0

Query: 25  SFHGENLSLADWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAPLVREEFAL 84
           SF   +LS  DW K  VL   S+ G V+LG   +  L +VAIK AP   ++ LV+E+  L
Sbjct: 6   SFKEYDLSAFDWDKTPVLGKGSH-GKVFLG---ELDLGSVAIKVAPCRAASSLVKEKAIL 65

Query: 85  AKLRPYPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYL 144
            +      IV+ IG T   +      +Y+L+LE+   GTL D+I+ +   +    A++YL
Sbjct: 66  KQFIGCSKIVQYIGDTVTKKGDD--VVYSLILEYATGGTLGDIIR-KQEKISEEDAKKYL 125

Query: 145 RTILQGLSSVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAP 204
           R ILQGLS +HA G+VH DLK DNILAF S  G    LKI++F L+R Y           
Sbjct: 126 RMILQGLSCIHAKGFVHADLKSDNILAFRSKSGR-MKLKIADFGLSRMYDEKEEKSLCWN 185

Query: 205 KRRLRFRGTPRYMSPESLTGEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLL 264
             ++ F GT +YMSPES+   +KPALD+WS+GC+FV+M+ G + +WN C   KE++SKL+
Sbjct: 186 YYKMSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTG-RHVWNDCKDNKELISKLI 245

Query: 265 DETATPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYL 308
            E   P +P+ELS  GK FL KCFERN ++RW+ DMLL HPYL
Sbjct: 246 YEREIPTIPKELSELGKDFLEKCFERNYEKRWSADMLLRHPYL 279

BLAST of Lag0002135 vs. ExPASy TrEMBL
Match: A0A6J1D9A6 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111018431 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 6.7e-55
Identity = 128/296 (43.24%), Postives = 175/296 (59.12%), Query Frame = 0

Query: 33  LADWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAPLVREEFALAKLRPYPH 92
           + +W +   L   SY G V+L  PT   L   A+K A     + L+ E+  L     +P 
Sbjct: 1   MTNWVRVXDLGKGSY-GKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPE 60

Query: 93  IVRSIGYTHHTRAQS-RFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTILQGL 152
           IV+  G    T   S     Y+LVLE+   GTLADLI+ +   L  +  + YL+ IL+GL
Sbjct: 61  IVQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIR-KQKKLSENTVKEYLKMILRGL 120

Query: 153 SSVHATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKRRLRFR 212
           S +H  G+VH DLKP NILAFP  DG+   LKI++F L+R  R   + + +    +L+FR
Sbjct: 121 SCIHKLGFVHVDLKPANILAFPQIDGK-MKLKIADFGLSR--RCNKKRECNF---KLKFR 180

Query: 213 GTPRYMSPESLTGEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLLDETATPD 272
           GTP YMSPES+ GE+   LD+WS+GC+ V+M+ G K  W+ C   ++++ KL     TP 
Sbjct: 181 GTPMYMSPESIYGEVNTPLDIWSLGCILVEMVTG-KHAWDDCIDLEQLMLKLRVNMETPK 240

Query: 273 VPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYLLERDEEEQILDETRCSQRLP 328
           +PEELS  GK FL KCF+RNPKRRWT DMLL HPY LE+++E    D+      LP
Sbjct: 241 IPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPY-LEQEKEAIKKDDDPTYSYLP 286

BLAST of Lag0002135 vs. ExPASy TrEMBL
Match: A0A6J1DIL5 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111021423 PE=4 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 5.3e-52
Identity = 125/278 (44.96%), Postives = 169/278 (60.79%), Query Frame = 0

Query: 36  WKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAPLVREEFALAKLRPYPHIVR 95
           W K + L   SY G V+L   T   +  VA+K AP   S+ L  E+  L  LR    IV+
Sbjct: 4   WTKVKELGRGSY-GKVFLAKSTIGPIDYVAVKVAPVERSSSLELEKDILQHLRGCSEIVQ 63

Query: 96  SIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTILQGLSSVH 155
            IG      ++     Y L+LE+   GTLA+LI+ +   L  S  + YLR IL+GLS +H
Sbjct: 64  CIG------SEMTSVCYYLILEYAGGGTLAELIR-KQKKLTESKVKEYLRMILRGLSCIH 123

Query: 156 ATGYVHCDLKPDNILAFPSADGESFDLKISEFSLARWYRSTWRSDDDAPKRRLRFRGTPR 215
             G+VH DLKP NILAFP  +G+   LKI++F L++  R     D+ A     RFRGT R
Sbjct: 124 RRGFVHADLKPANILAFPRPNGK-MKLKIADFGLSK--RWVVGQDEAAEHSEPRFRGTAR 183

Query: 216 YMSPESLT-GEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLLDETATP-DVP 275
           YMSPES+  G+I P LD+WS+GC+F++MI G+  +WN C T  ++L KL++ET  P DV 
Sbjct: 184 YMSPESIIFGKISPPLDIWSLGCIFIEMITGSP-VWNSCRTYDQLLKKLVNETELPTDVL 243

Query: 276 EELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYLLERD 312
           ++LS  G+ FL  C +R+ KRRWT DMLL HPYL E +
Sbjct: 244 KKLSKEGRDFLASCLDRDSKRRWTADMLLKHPYLSEEN 269

BLAST of Lag0002135 vs. ExPASy TrEMBL
Match: A0A5N6QLW2 (Protein kinase domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_004658 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 9.0e-52
Identity = 131/309 (42.39%), Postives = 178/309 (57.61%), Query Frame = 0

Query: 33  LADWKKARVLSTASYGGGVYLGIPTKPHLATVAIKSAPFNDSAPLVREEFALAKLRPYPH 92
           +A W++ RVL   +Y G V+L  PT P    +A+K+ P   S+ L  EE  LA+LR  P 
Sbjct: 1   MAMWQRRRVLGKGTY-GTVFLAEPTDPTAPPIAVKTCPIAKSSSLHLEERVLARLRGCPD 60

Query: 93  IVRSIGYTHHTRAQSRFGIYNLVLEHTPTGTLADLIQARTNNLPHSLARRYLRTILQGLS 152
           IV  +G +     +   G+YNL LE+   GTLADLI+ + + L     RRY+R IL+GL 
Sbjct: 61  IVSCLGSS--LTVEDGTGLYNLFLEYAAGGTLADLIK-KESGLCERDMRRYVRMILRGLC 120

Query: 153 SVHATGYVHCDLKPDNILAFPSADG--ESFDLKISEFSLARWYRSTWRSDDDAPKRRLRF 212
           SVH  G VHCDLKP NIL FP   G   +  LKI++F LA+         ++     L F
Sbjct: 121 SVHGKGMVHCDLKPSNILVFPGEGGVLNADRLKIADFGLAK------EGKEEMGFWGLNF 180

Query: 213 RGTPRYMSPESL-TGEIKPALDVWSVGCVFVDMINGNKGIWNGCSTRKEVLSKLL-DETA 272
           RGTP YMSPES+ +G+I PALD+WS+GC+ V+M+ G   +W       + L+ LL     
Sbjct: 181 RGTPFYMSPESVASGDIGPALDIWSLGCILVEMVTGKPALWK--YRNLDPLNLLLVIAKQ 240

Query: 273 TPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLLDHPYLLERDEEEQILDETRCSQRLPF 332
           +P +PE LS  GK FLR CF  NP +RWT  MLL+HP+ + R    +  DE   S + P+
Sbjct: 241 SPGIPEGLSEDGKDFLRNCFAMNPCQRWTAKMLLNHPF-VSRPMYGKTPDEPPASPKSPW 293

Query: 333 FVGRLCSAS 338
               LC +S
Sbjct: 301 ---DLCPSS 293

BLAST of Lag0002135 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 159.5 bits (402), Expect = 5.1e-39
Identity = 103/282 (36.52%), Postives = 148/282 (52.48%), Query Frame = 0

Query: 54  GIPTKPHLATVAIKSAPFNDSAPLVREEFALAKLRPYPHIVRSIGYTHHTRAQSRFGIYN 113
           G  +K   + +A+KS+    SA L  E   L  L     IVR  G       ++   IYN
Sbjct: 24  GSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCSEIVRCFG--EGRTVENGEEIYN 83

Query: 114 LVLEHTPTGTLADLIQARTNNLPHSLARRYLRTILQGLSSVHATGYVHCDLKPDNILAFP 173
           L LE+   G+LAD I++    LP    RR+ R+I++GL  +H  G+ HCD+K +N+L F 
Sbjct: 84  LFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGLCHIHGNGFTHCDIKLENVLVF- 143

Query: 174 SADGESFDLKISEFSLARWYRSTWRSDDDAPKRRLRFRGTPRYMSPESLT-GEIKPALDV 233
             DG   D+KIS+F LA+      RS +      +  RGTP YM+PES+  GE +   D+
Sbjct: 144 -GDG---DVKISDFGLAK-----RRSGEVC----VEIRGTPLYMAPESVNHGEFESPADI 203

Query: 234 WSVGCVFVDMING------NKGIWNGCSTRKEVLSKLLDETATPDVPEELSAGGKYFLRK 293
           W++GC  V+M +G        G+ N   +   +L ++      P +P ELS  GK F+ K
Sbjct: 204 WALGCSVVEMSSGKTAWCLEDGVMNNVMS---LLVRIGSGDEVPRIPVELSEEGKDFVSK 263

Query: 294 CFERNPKRRWTVDMLLDHPYLLERDEEEQILDETRCSQRLPF 329
           CF +N   RWT +MLLDHP+L   DE  +  +    S R PF
Sbjct: 264 CFVKNAAERWTAEMLLDHPFLAVDDESGEEDEACSVSPRNPF 286

BLAST of Lag0002135 vs. TAIR 10
Match: AT2G42550.1 (Protein kinase superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 5.6e-38
Identity = 96/250 (38.40%), Postives = 135/250 (54.00%), Query Frame = 0

Query: 65  AIKSAPFNDSAPLVREEFALAKLRPYPHIVRSIG-YT-HHTRAQSRFGIYNLVLEHTPTG 124
           A+K+A   D   L RE   L+KL     IV+  G YT         F +Y +V+E+   G
Sbjct: 37  AVKTAECEDYNSLEREIQILSKLEGCRRIVQCYGNYTLEEDFDVGGFRVYKMVMEYAAAG 96

Query: 125 TLADLIQA-RTNNLPHSLARRYLRTILQGLSSVHATGYVHCDLKPDNILAFPSADGESFD 184
           +L   + + +   LP ++ + + R ILQGL SVH  GYVHCDLKPDN+L FP    +S++
Sbjct: 97  SLFSFMDSYKDRKLPETMIKDFTRMILQGLVSVHRLGYVHCDLKPDNLLVFPCR--QSYE 156

Query: 185 LKISEFSLAR---WYRSTWRSDDDAPKRRLRFRGTPRYMSPESL-TGEIKPALDVWSVGC 244
           LKIS+F  +R    Y   W  D       L F GTP YMSPES+ +G  + ALD+WS+GC
Sbjct: 157 LKISDFGSSRKVGEYSDCWDVD-------LPFVGTPVYMSPESVRSGVAEKALDLWSLGC 216

Query: 245 VFVDMINGNKGIWNGCSTRKEVLSKLLDETATPDVPEELSAGGKYFLRKCFERNPKRRWT 304
           + ++M     G+        E L+  L +   P++P+ L    + FL  CF RNPK R +
Sbjct: 217 IVLEMYT---GVIPWSEVEFEDLAPALSKGKAPEIPKSLPCDARKFLETCFSRNPKERGS 274

Query: 305 VDMLLDHPYL 308
              LL H +L
Sbjct: 277 ASDLLSHQFL 274

BLAST of Lag0002135 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-36
Identity = 99/258 (38.37%), Postives = 142/258 (55.04%), Query Frame = 0

Query: 65  AIKSAPFNDSAPLVREEFALAKLRPYPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPTGTL 124
           A+KSA F+ SA L RE+  L+KL   P+IV+ IG ++ T+   +  +YNL++E+   G+L
Sbjct: 32  AVKSAEFSSSAFLQREQSILSKLSS-PYIVKYIG-SNVTKENDKL-MYNLLMEYVSGGSL 91

Query: 125 ADLIQARTNNLPHSLARRYLRTILQGLSSVHATGYVHCDLKPDNILAFPSADGESFDLKI 184
            DLI+     LP  L R Y R IL+GL  +H  G VHCD+K  N++      GE    KI
Sbjct: 92  HDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQGIVHCDVKSQNVM----IGGEI--AKI 151

Query: 185 SEFSLARWYRSTWRSDDDAPKRRLRFRGTPRYMSPESLTGEIK--PALDVWSVGCVFVDM 244
            +   A+                L F GTP +MSPE   GE +  PA DVW++GC  ++M
Sbjct: 152 VDLGCAKTVEE---------NENLEFSGTPAFMSPEVARGEEQSFPA-DVWALGCTVIEM 211

Query: 245 INGNKGIWNGCSTRKEVLSKLLDETATPDVPEELSAGGKYFLRKCFERNPKRRWTVDMLL 304
             G+   W   +     + K+     +P +P  LS  G+ FLRKC  ++PK+RWTV+ LL
Sbjct: 212 ATGSSP-WPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQRWTVEELL 266

Query: 305 DHPYLLERDEEEQILDET 321
            HP+L   DEE+   D+T
Sbjct: 272 QHPFL---DEEDNDSDQT 266

BLAST of Lag0002135 vs. TAIR 10
Match: AT5G27510.1 (Protein kinase superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.6e-35
Identity = 93/255 (36.47%), Postives = 137/255 (53.73%), Query Frame = 0

Query: 65  AIKSAPFNDSAPLVREEFALAKLRPYPHIVRSIGYTHHTRAQSRFG-IYNLVLEHTPTGT 124
           A+KS+  +D   L++E   L++L+  P I++  G         +   +Y L+LE+   G+
Sbjct: 35  AVKSS--DDENSLLKEFHILSELKGCPRIIQCFGNDLEEGFDDKGNRVYKLLLEYASEGS 94

Query: 125 LADLI-QARTNNLPHSLARRYLRTILQGLSSVHATGYVHCDLKPDNILAFPSADGESFDL 184
           L+D +       LP  + R + R ILQGL S+H+ GYVHCDLKP+N+L FP   G+S+++
Sbjct: 95  LSDFMNNCVDRKLPDLMIRDFTRMILQGLVSIHSHGYVHCDLKPENVLVFPC--GDSYEV 154

Query: 185 KISEFSLARWYRSTWRSDDDAPKRRLRFRGTPRYMSPESL-TGEIKPALDVWSVGCVFVD 244
           KIS+F L+           D  K    F GT  YM PESL  G     LD+WS+GC+ ++
Sbjct: 155 KISDFGLSLQVGEV----PDHWKIEYPFVGTLNYMPPESLHDGVANKTLDLWSLGCLVLE 214

Query: 245 MINGNKGIWNGCSTRKEVLSKLLDETATPDVPEELSAGGKYFLRKCFERNPKRRWTVDML 304
           M    K  W G      V   +L     P++PE L    + F++KCF RNPK R T   L
Sbjct: 215 MYVCKKP-WIGFIPEDFVY--ILSNGNPPEIPESLPCDARAFIQKCFSRNPKERGTASEL 274

Query: 305 LDHPYLLERDEEEQI 317
           L H +L  R E+ ++
Sbjct: 275 LSHRFL--RQEKSKL 276

BLAST of Lag0002135 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 146.7 bits (369), Expect = 3.4e-35
Identity = 100/282 (35.46%), Postives = 149/282 (52.84%), Query Frame = 0

Query: 62  ATVAIKSAPFNDSAPLVREEFALAKLRPYPHIVRSIGYTHHTRAQSRFGIYNLVLEHTPT 121
           A +A+KS     +A L  E+  L  L   P I+R   Y   +  ++   ++NL+LE+   
Sbjct: 31  ALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRC--YGEDSTVENGEEMHNLLLEYASR 90

Query: 122 GTLADLI-QARTNNLPHSLARRYLRTILQGLSSVHATGYVHCDLKPDNILAFPSADGESF 181
           G+LA  + +     LP S  RR+  ++L+GL  +HA G+ HCD+K  NIL F   DG   
Sbjct: 91  GSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKGFAHCDIKLANILLF--NDG--- 150

Query: 182 DLKISEFSLARWYRSTWRSDDD--APKRRLRFRGTPRYMSPESLT-GEIKPALDVWSVGC 241
            +KI++F LA       R D D  A ++ +  RGTP YM+PE +   E   A DVW++GC
Sbjct: 151 SVKIADFGLA------MRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGC 210

Query: 242 VFVDMINGNKGIWN--GCSTRKEVLSKLLDETATPDVPEELSAGGKYFLRKCFERNPKRR 301
             V+M +G K  W+    S    +L ++      P +PE LS  GK FL KCF ++P +R
Sbjct: 211 AVVEMFSG-KTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKR 270

Query: 302 WTVDMLLDHPYL---LERDEEEQIL------DETRCSQRLPF 329
           WT +MLL+H ++   LE D  E  +      D+   S + PF
Sbjct: 271 WTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPF 298