Lag0002103 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002103
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	pectinesterase inhibitor-like
Location	chr4: 39331973 .. 39332491 (+)
RNA-Seq Expression	Lag0002103
Synteny	Lag0002103

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAATTCCATCATCCTTGTAGCTTTCGTCCTTTCACTTGTTTTGTCCACGCAAGCCGCTTCCTCAAACGACGTCGTCTCCACCATCTGTTCAAGAACTAGAAACCCTCCCTTTTGCACCAACGTCTTGAAATCTGCTGGCACCACAGAGCTGAAAGGGCTGGCCACCTTCACCCTGAACCTGGCCCGCACGAGGGCTGGCGAGAGCCGCGTCCTGGCACTGTCCCTAGCGTCCAAGGCCGTCGATCCCAAGCTCAAGGAGCGCTACGCCTCTTGCACCGAAAACTACGACGATGCCGTTGGCGACATCGACGATGCCGGGAAGGACTTGGCGTCGGGTGACTACAATGGCGTCAACGTTCACGCTTCTGGAGCCATGACCGAGGCCGATGACTGCCAGGACAACTTCGCGCAGCCGCCGACGGACCCGTCGGCGCTGTCCGCCAACGCTAAGGCTTTGGAAGATATTTGTAGTATTATCTTGGTCATAGCTAATCTTCTCCTTGGCCGCATTTGA

mRNA sequence

ATGGCGAATTCCATCATCCTTGTAGCTTTCGTCCTTTCACTTGTTTTGTCCACGCAAGCCGCTTCCTCAAACGACGTCGTCTCCACCATCTGTTCAAGAACTAGAAACCCTCCCTTTTGCACCAACGTCTTGAAATCTGCTGGCACCACAGAGCTGAAAGGGCTGGCCACCTTCACCCTGAACCTGGCCCGCACGAGGGCTGGCGAGAGCCGCGTCCTGGCACTGTCCCTAGCGTCCAAGGCCGTCGATCCCAAGCTCAAGGAGCGCTACGCCTCTTGCACCGAAAACTACGACGATGCCGTTGGCGACATCGACGATGCCGGGAAGGACTTGGCGTCGGGTGACTACAATGGCGTCAACGTTCACGCTTCTGGAGCCATGACCGAGGCCGATGACTGCCAGGACAACTTCGCGCAGCCGCCGACGGACCCGTCGGCGCTGTCCGCCAACGCTAAGGCTTTGGAAGATATTTGTAGTATTATCTTGGTCATAGCTAATCTTCTCCTTGGCCGCATTTGA

Coding sequence (CDS)

ATGGCGAATTCCATCATCCTTGTAGCTTTCGTCCTTTCACTTGTTTTGTCCACGCAAGCCGCTTCCTCAAACGACGTCGTCTCCACCATCTGTTCAAGAACTAGAAACCCTCCCTTTTGCACCAACGTCTTGAAATCTGCTGGCACCACAGAGCTGAAAGGGCTGGCCACCTTCACCCTGAACCTGGCCCGCACGAGGGCTGGCGAGAGCCGCGTCCTGGCACTGTCCCTAGCGTCCAAGGCCGTCGATCCCAAGCTCAAGGAGCGCTACGCCTCTTGCACCGAAAACTACGACGATGCCGTTGGCGACATCGACGATGCCGGGAAGGACTTGGCGTCGGGTGACTACAATGGCGTCAACGTTCACGCTTCTGGAGCCATGACCGAGGCCGATGACTGCCAGGACAACTTCGCGCAGCCGCCGACGGACCCGTCGGCGCTGTCCGCCAACGCTAAGGCTTTGGAAGATATTTGTAGTATTATCTTGGTCATAGCTAATCTTCTCCTTGGCCGCATTTGA

Protein sequence

MANSIILVAFVLSLVLSTQAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELKGLATFTLNLARTRAGESRVLALSLASKAVDPKLKERYASCTENYDDAVGDIDDAGKDLASGDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGRI

Homology

[Close]

BLAST of Lag0002103 vs. NCBI nr
Match: XP_038896410.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 228.0 bits (580), Expect = 6.1e-56
Identity = 130/182 (71.43%), Postives = 142/182 (78.02%), Query Frame = 0


Query: 1   MANSIILVA-----FVLSL-----VLSTQAASSNDVVSTICSRTRNPPFCTNVLKSAGTT 60
           MANSIIL A     FVLSL     V+   AAS N+VVS IC RT NPPFC+N+LKSA TT
Sbjct: 1   MANSIILTASSLLPFVLSLLFFHHVVPMHAASQNNVVSIICQRTGNPPFCSNLLKSARTT 60

Query: 61  ELKGLATFTLNLARTRAGESRVLALSLASKAVDPKLKERYASCTENYDDAVGDIDDAGKD 120
           +LKGLATFTLNLART A +SR LA  LA KA DPKLKERYASC E+YDDA GD+DDA   
Sbjct: 61  DLKGLATFTLNLARTNATKSRALAQFLALKAADPKLKERYASCAEHYDDAAGDLDDAKNY 120

Query: 121 LASGDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLG 173
           LA+GDYNGVN+ ASGAMTE DDCQDNF Q P D S LS N KALEDICSIILVIAN+LLG
Sbjct: 121 LATGDYNGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKNGKALEDICSIILVIANILLG 180

[Close]

BLAST of Lag0002103 vs. NCBI nr
Match: XP_038896411.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 222.6 bits (566), Expect = 2.6e-54
Identity = 126/182 (69.23%), Postives = 142/182 (78.02%), Query Frame = 0


Query: 1   MANSIILVA-----FVLSL-----VLSTQAASSNDVVSTICSRTRNPPFCTNVLKSAGTT 60
           MANSIIL A     FVLSL     V+   AAS NDVVSTIC RTRNPPFC+N+LKSA TT
Sbjct: 1   MANSIILPASSLLPFVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTT 60

Query: 61  ELKGLATFTLNLARTRAGESRVLALSLASKAVDPKLKERYASCTENYDDAVGDIDDAGKD 120
           +LKGL TFT+NLA T+A ESR LA  LASKA DPK KERYASC ++YDDA  D++DA   
Sbjct: 61  DLKGLITFTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNY 120

Query: 121 LASGDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLG 173
           LA+GDY+GVN+ ASGAMTE DDCQDNF Q P D S LS + KALEDICSIILVIAN+LLG
Sbjct: 121 LATGDYDGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILLG 180

[Close]

BLAST of Lag0002103 vs. NCBI nr
Match: XP_022949093.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 221.1 bits (562), Expect = 7.5e-54
Identity = 113/152 (74.34%), Postives = 125/152 (82.24%), Query Frame = 0


Query: 20  AASSNDVVSTICSRTRNPPFCTNVLKSAGTTELKGLATFTLNLARTRAGESRVLALSLAS 79
           AAS +D+VSTIC +TRNP FC NVLKSAGTT+LKGLATFTLNLA  + G++R LALSLAS
Sbjct: 3   AASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKVGQTRALALSLAS 62

Query: 80  KAVDPKLKERYASCTENYDDAVGDIDDAGKDLASGDYNGVNVHASGAMTEADDCQDNFAQ 139
           KA DPKLKERYA+C E YDDA  DI+D   DL  GDYNGVN+ AS AMTEA DC D+F Q
Sbjct: 63  KAADPKLKERYATCAEQYDDAADDIEDGKNDLGEGDYNGVNIKASAAMTEAGDCLDSFTQ 122

Query: 140 PPTDPSALSANAKALEDICSIILVIANLLLGR 172
           PP DPSALS N K +EDICSIILVIANLLLGR
Sbjct: 123 PPKDPSALSGNGKTVEDICSIILVIANLLLGR 154

[Close]

BLAST of Lag0002103 vs. NCBI nr
Match: XP_023521203.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 220.3 bits (560), Expect = 1.3e-53
Identity = 115/167 (68.86%), Postives = 133/167 (79.64%), Query Frame = 0


Query: 5   IILVAFVLSLVLSTQAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELKGLATFTLNLAR 64
           I+L+    + ++  QAAS +D+VSTIC +TRNP FC NVLKSAGTT+LKGLATFTLNLA 
Sbjct: 12  IVLLVLFFNGMVPMQAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAH 71

Query: 65  TRAGESRVLALSLASKAVDPKLKERYASCTENYDDAVGDIDDAGKDLASGDYNGVNVHAS 124
            +  ++R LA SLASKAVDPKLKERYA+C E YDDA GDI+D   +L  GDYNGVN+ AS
Sbjct: 72  DKVAQTRALAQSLASKAVDPKLKERYATCAEQYDDAAGDIEDGKNNLGKGDYNGVNIKAS 131

Query: 125 GAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGR 172
            AMTEA DC D+F QPP DPSALS N K +EDICSIILVIANLLLGR
Sbjct: 132 AAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Lag0002103 vs. NCBI nr
Match: XP_023524536.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 218.8 bits (556), Expect = 3.7e-53
Identity = 121/178 (67.98%), Postives = 137/178 (76.97%), Query Frame = 0


Query: 1   MANSIILVAF-VLSLVL------STQAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELK 60
           MA+S  L  F ++ LVL        QAAS +D+VSTIC +TRNP FC NVLKSAGTT+LK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMGPMQAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLARTRAGESRVLALSLASKAVDPKLKERYASCTENYDDAVGDIDDAGKDLAS 120
           GLATFTLNLA  +  ++R LA S ASKAVDPKLKERYA+C E YDDA GDI+D   DL  
Sbjct: 61  GLATFTLNLAHDKVAQTRALAQSQASKAVDPKLKERYATCAEQYDDAAGDIEDGKNDLGK 120

Query: 121 GDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGR 172
           GDYNGVN+ AS AMTEA DC D+F QPP DPSALS+N K +EDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSSNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Lag0002103 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 3.6e-27
Identity = 67/169 (39.64%), Postives = 104/169 (61.54%), Query Frame = 0


Query: 5   IILVAFVLSLVLSTQAASSNDVVSTICSRTRNPPFCTNVLKS---AGTTELKGLATFTLN 64
           ++++ F+  L       + N ++S IC +TRNP  C   L+S   + + +LKGL  F+++
Sbjct: 15  LLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLKGLGQFSID 74

Query: 65  LARTRAGESRVLALSLASKAVDPKLKERYASCTENYDDAVGDIDDAGKDLASGDYNGVNV 124
           +A+  A ++  +  SL ++A DPKLK RY +C+ENY DA+  +  A + L SGDYN +N+
Sbjct: 75  IAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTSGDYNSLNI 134

Query: 125 HASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLG 171
           +AS A   A  C+D+F  PP  P+ L      LED+C I+LVI+NLL G
Sbjct: 135 YASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of Lag0002103 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.9e-18
Identity = 56/169 (33.14%), Postives = 99/169 (58.58%), Query Frame = 0


Query: 1   MANSIILVAFVLSLVLSTQAASSNDVVSTICSRTRNPPFCTNVLKSA-GTTELKGLATFT 60
           + N+  L + +  L++ +  A ++  +STIC +T NP FC   L +   +  L+ LA  T
Sbjct: 5   LRNNAFLSSLMFLLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTT 64

Query: 61  LNLARTRAGESRVLALSLASKAVDPKLKERYASCTENYDDAVGDIDDAGKDLASGDYNGV 120
           L+  + RA ++     S+    VDP+ K  Y SC + Y+ A+G++++A + LASGD  G+
Sbjct: 65  LDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGM 124

Query: 121 NVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLL 169
           N+  S A+  AD C D+  +  +  S++  N+K ++++C I LVI+N+L
Sbjct: 125 NMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Lag0002103 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.2e-17
Identity = 59/165 (35.76%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 7   LVAFVLSLVLSTQAASSNDVVSTICSRTRNPPFCTNVLKSAGTT---ELKGLATFTLNLA 66
           L+ FV++  L+ Q A     +  IC + +N  FCT+ +KS   T   +L+ LA  T   A
Sbjct: 14  LMFFVMTGSLNAQVAD----IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSA 73

Query: 67  RTRAGESRVLALSLASKAVDPKLKERYASCTENYDDAVGDIDDAGKDLASGDYNGVNVHA 126
           +T A E      SL   A +P +K+ Y SC ++Y  A+  ++DA + LASGD  G+N+  
Sbjct: 74  QTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKV 133

Query: 127 SGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLL 169
           S AM     C+ + A    DPSA+  N+   ++IC I+LVI+N++
Sbjct: 134 SAAMEGPSTCEQDMADFKVDPSAVK-NSGDFQNICGIVLVISNMM 173

The following BLAST results are available for this feature:

BLAST of Lag0002103 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896410.1	6.1e-56	71.43	pectinesterase inhibitor-like [Benincasa hispida]	[more]
XP_038896411.1	2.6e-54	69.23	pectinesterase inhibitor-like [Benincasa hispida]	[more]
XP_022949093.1	7.5e-54	74.34	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
XP_023521203.1	1.3e-53	68.86	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_023524536.1	3.7e-53	67.98	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0002103 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	3.6e-27	39.64	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LNF2	8.9e-18	33.14	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]
Q9LUV1	1.2e-17	35.76	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 22..164 e-value: 7.8E-27 score: 105.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 26..164 e-value: 2.5E-21 score: 76.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..168 e-value: 6.7E-35 score: 118.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 21..168 e-value: 3.5E-36 score: 126.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 24..169
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 2..168
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 2..168
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 24..168 e-value: 1.11549E-43 score: 140.25

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002103.1	Lag0002103.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity