Lag0001991 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0001991 vs. NCBI nr
Match: XP_022156590.1 (putative lysine-specific demethylase JMJ16 [Momordica charantia])

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 681/827 (82.35%), Postives = 741/827 (89.60%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           MDEDC+RNSLNQGVENLL PPGFISRRSFRLRRVEQNE+DDS K ++ EI  LS+   IE
Sbjct: 1   MDEDCSRNSLNQGVENLLAPPGFISRRSFRLRRVEQNENDDSSKIKQTEISALSQTIGIE 60

Query: 61  -MEAACRKRPWILFNQ-NKDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
            MEAACR+RPWILF+Q N+DSLEFE ME DMNLPPQSDLPKGVAHGCPECSNC+KVTARW
Sbjct: 61  MMEAACRQRPWILFDQKNEDSLEFESMENDMNLPPQSDLPKGVAHGCPECSNCVKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
           HPE ARTD LE+APVFYPTEEEFADTL YIERIRPRAE CGICRIVPPPSWLPPC LKEK
Sbjct: 121 HPEDARTDSLEEAPVFYPTEEEFADTLQYIERIRPRAESCGICRIVPPPSWLPPCLLKEK 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWENSPFLAHYQRIDGFQKN+ACG+FP+H+ N+KN+ RR+EYEC NRC MDPDESCG+D
Sbjct: 181 EIWENSPFLAHYQRIDGFQKNFACGRFPKHNDNVKNKRRRIEYECGNRCFMDPDESCGID 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            KGPNS+LGRE TLKAFKSYADDFKSQYFS GNKVT+TE NS  +QEQWEP+VDQVE EY
Sbjct: 241 KKGPNSKLGREFTLKAFKSYADDFKSQYFSSGNKVTDTETNSSTVQEQWEPLVDQVESEY 300

Query: 301 RRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSL 360
           RRIVENPTEQIEVLCG SL S SLGSGFP SSSPLIEPGHADHM+SGWNLNNL RLPGSL
Sbjct: 301 RRIVENPTEQIEVLCGHSLDSPSLGSGFPASSSPLIEPGHADHMESGWNLNNLRRLPGSL 360

Query: 361 LSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFE 420
           LSFDSFKTCS+L P+VYVGMCFS+ CWRVEEHHLPLLCYLHLGAPKIWYGIPGRY  +F+
Sbjct: 361 LSFDSFKTCSILLPQVYVGMCFSSTCWRVEEHHLPLLCYLHLGAPKIWYGIPGRYIGQFD 420

Query: 421 VAMKSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNC 480
           VAMK+LPE F+EQK F    VSQPSIA LKREG+P+YRCIQNPGEFVLVFPG CHSGFNC
Sbjct: 421 VAMKNLPEFFVEQKRFHRGMVSQPSIAMLKREGLPVYRCIQNPGEFVLVFPGACHSGFNC 480

Query: 481 GFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRK 540
           GFS++EEANFAPL WLP+GYNA+ELY+++ RKT ISFDRLLL AA+EAVKAQWELSLCRK
Sbjct: 481 GFSITEEANFAPLHWLPYGYNAVELYAIDGRKTSISFDRLLLRAAIEAVKAQWELSLCRK 540

Query: 541 ETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSIC 600
           ETK+NLRWK+ACGK+G+LAQTFKSRIKSEGLRREYLT+ASQM+EV E++DAV KRECSIC
Sbjct: 541 ETKENLRWKDACGKNGVLAQTFKSRIKSEGLRREYLTSASQMQEVNENYDAVCKRECSIC 600

Query: 601 LYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAV 660
           LYDLH SAAGC CS DRYSCLNHAKQLCSCAWG +FF+VR+KMG+LNLLLEALEGKLSAV
Sbjct: 601 LYDLHLSAAGCPCSADRYSCLNHAKQLCSCAWGDKFFVVRYKMGNLNLLLEALEGKLSAV 660

Query: 661 YKWAKENLGLAVHSY--KNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKA 720
           YKW KENLGLAVHSY  KNS PQS  ADS  SSKG QSED+E  N+ N+SI RIKAEIKA
Sbjct: 661 YKWVKENLGLAVHSYITKNSLPQSQTADSSHSSKGDQSEDAELPNSANTSIIRIKAEIKA 720

Query: 721 RLLQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEK--TASKLQPVSSNELKGKE 780
           RLLQS TLK  K+NEKVIESEDAVK NG  +NSDS IP EK  TAS LQP+SSNELKGKE
Sbjct: 721 RLLQSTTLKRMKKNEKVIESEDAVKYNGNPSNSDSGIPLEKAATASMLQPISSNELKGKE 780

Query: 781 NTSTPAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDDCWSDSG 822
             STPAV LNERGDGLIFSLNL+SSE LSESS SSFS+ DDCWSDSG
Sbjct: 781 KPSTPAVALNERGDGLIFSLNLESSEILSESSASSFSDLDDCWSDSG 827

BLAST of Lag0001991 vs. NCBI nr
Match: XP_023515071.1 (putative lysine-specific demethylase JMJ16 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1379.8 bits (3570), Expect = 0.0e+00
Identity = 683/822 (83.09%), Postives = 733/822 (89.17%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           MDEDCTR+SLNQGVENLL PPGFISRR+FRLRRVEQNE+D SI+T+K +I TLSR  DI+
Sbjct: 1   MDEDCTRSSLNQGVENLLAPPGFISRRAFRLRRVEQNENDGSIQTKKTKIDTLSRKIDID 60

Query: 61  M-EAACRKRPWILFNQ-NKDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
           + EA CR+RPWILFNQ N+DSLEFEPM+ D++LPPQSDLPKGVAHGCPECSNCLKVTARW
Sbjct: 61  LVEATCRQRPWILFNQNNEDSLEFEPMQHDVDLPPQSDLPKGVAHGCPECSNCLKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
           HPE ART+IL++APVFYP+EEEF+DTL YIERIR RAE  GICRIVPPPSWLPPC LKE 
Sbjct: 121 HPEDARTNILDEAPVFYPSEEEFSDTLSYIERIRSRAESSGICRIVPPPSWLPPCLLKET 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWE+SPFLAH QRIDG QK+YACG+  +HS NI N+ RRLEYEC NRCL D DESCG D
Sbjct: 181 EIWEDSPFLAHCQRIDGIQKDYACGRLAKHSKNITNKRRRLEYECGNRCLKDSDESCGSD 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            KGP SELG+E TLKAFKSYADDFKSQYFS GNKVT+TE  S +LQEQWEP+VDQ+EGEY
Sbjct: 241 KKGPYSELGQEFTLKAFKSYADDFKSQYFSSGNKVTSTETKSSMLQEQWEPLVDQIEGEY 300

Query: 301 RRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSL 360
           R IVENPTEQIEVL G SLGS SLGSGFP S S  IEPGH DHMDSGWNLNNL RLPGSL
Sbjct: 301 RHIVENPTEQIEVLYGDSLGSPSLGSGFPSSPSSPIEPGHVDHMDSGWNLNNLPRLPGSL 360

Query: 361 LSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFE 420
           LSFDSFKTCS+LSPRV+VGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRY DKFE
Sbjct: 361 LSFDSFKTCSILSPRVHVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYIDKFE 420

Query: 421 VAMKSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNC 480
           VAMKSLPE F+EQK      VSQPSIATLKREGIPIYRCIQNPGEFVLV PG CHSGFNC
Sbjct: 421 VAMKSLPETFVEQKRSHRGMVSQPSIATLKREGIPIYRCIQNPGEFVLVLPGTCHSGFNC 480

Query: 481 GFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRK 540
           GFSV+EEANFAPLDWLP+GYNAMELYSVERRKT +SFDRLLLGA +E VKAQWELSLCRK
Sbjct: 481 GFSVTEEANFAPLDWLPYGYNAMELYSVERRKTFVSFDRLLLGAVIETVKAQWELSLCRK 540

Query: 541 ETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSIC 600
           +T DNLRWKNACGK+GILAQTFKSRI SEGLRREYLTTASQ+REVTE FDAV KRECSIC
Sbjct: 541 DTSDNLRWKNACGKNGILAQTFKSRITSEGLRREYLTTASQVREVTEIFDAVRKRECSIC 600

Query: 601 LYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAV 660
           LYDLH SAAGCSCS DRYSCLNHAKQLCSCAWG +FF+VR+KMGSLNLLLEALEGKLSAV
Sbjct: 601 LYDLHLSAAGCSCSADRYSCLNHAKQLCSCAWGDKFFVVRYKMGSLNLLLEALEGKLSAV 660

Query: 661 YKWAKENLGLAVHSYKNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKARL 720
           YKWAKENLGLAVHSYKNSS QS P DSPQSSK  QSED+E+ N VN+SI RIKAEIKARL
Sbjct: 661 YKWAKENLGLAVHSYKNSSLQSHPPDSPQSSKSHQSEDAETPNAVNNSILRIKAEIKARL 720

Query: 721 LQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEKTASKLQPVSSNELKGKENTST 780
           LQSKTLK+ KE E +IE +DAVKDNG LTNS S IPA+K  SKLQPVSSNELKGKE+ ST
Sbjct: 721 LQSKTLKHSKETETMIEPKDAVKDNGILTNSCSSIPADKAVSKLQPVSSNELKGKEDRST 780

Query: 781 PAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDDCWSDS 821
           PAVVLNER DGL FSLNL+SSE LSESS SSFSESD  WSDS
Sbjct: 781 PAVVLNERKDGLTFSLNLESSEILSESSTSSFSESDGYWSDS 822

BLAST of Lag0001991 vs. NCBI nr
Match: XP_022960375.1 (putative lysine-specific demethylase JMJ16 [Cucurbita moschata] >XP_022960376.1 putative lysine-specific demethylase JMJ16 [Cucurbita moschata] >XP_022960377.1 putative lysine-specific demethylase JMJ16 [Cucurbita moschata])

HSP 1 Score: 1364.0 bits (3529), Expect = 0.0e+00
Identity = 676/822 (82.24%), Postives = 728/822 (88.56%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           MDEDCTR+SLNQG+ENLL PPGF SRRSFRLRRVEQ E+D SI+T+K +I TLSR  DI+
Sbjct: 1   MDEDCTRSSLNQGLENLLAPPGFTSRRSFRLRRVEQKENDGSIQTKKTKIDTLSRKIDID 60

Query: 61  M-EAACRKRPWILFNQ-NKDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
           + EA CR+RPWILFNQ N+DSLEFE M+ D++ PPQSDLPKGVAHGCPECSNCLKVTARW
Sbjct: 61  LVEATCRQRPWILFNQNNEDSLEFESMQHDVDPPPQSDLPKGVAHGCPECSNCLKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
           HPE ART+IL++APVFYP+EEEF+DTL YIERIR RAE  GICRIVPPPSWLPPC LKE 
Sbjct: 121 HPEDARTNILDEAPVFYPSEEEFSDTLSYIERIRSRAESSGICRIVPPPSWLPPCLLKET 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWE+SPFLAH QRIDG QK+YACG+  +HS NI N+ RRLEYEC NRCL D DESCG D
Sbjct: 181 EIWEDSPFLAHCQRIDGIQKDYACGRLAKHSKNITNKRRRLEYECGNRCLKDSDESCGSD 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            KGP SELG+E TLKAFKSYADDFKSQYFS GNKVT+TE  S +LQEQWEP+VDQ+EGEY
Sbjct: 241 KKGPYSELGQEFTLKAFKSYADDFKSQYFSSGNKVTSTETKSSMLQEQWEPLVDQIEGEY 300

Query: 301 RRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSL 360
           R IVENPTEQIEVL G SLGS SLGSGFP S S  IEPGH DHMDSGWNLNNL RLPGSL
Sbjct: 301 RHIVENPTEQIEVLYGDSLGSPSLGSGFPSSPSSPIEPGHVDHMDSGWNLNNLPRLPGSL 360

Query: 361 LSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFE 420
           LSFDSFKTCS+LSPRV+VGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRY DKFE
Sbjct: 361 LSFDSFKTCSILSPRVHVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYIDKFE 420

Query: 421 VAMKSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNC 480
           VAMKSLPE F+EQK      VSQPSIATLKR GIPIYRCIQNPGEFVLV PG CHSGFNC
Sbjct: 421 VAMKSLPETFVEQKRSHRGMVSQPSIATLKRVGIPIYRCIQNPGEFVLVLPGTCHSGFNC 480

Query: 481 GFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRK 540
           GFSV+EEANFAPLDWLP+GYNAMELYSVERRKT++SFDRLLLGA +E VKAQWELSLCRK
Sbjct: 481 GFSVTEEANFAPLDWLPYGYNAMELYSVERRKTVVSFDRLLLGAVIETVKAQWELSLCRK 540

Query: 541 ETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSIC 600
           +T DNLRWKNACGK+GILAQTFKSRI SEGLRREYLTTASQ+REVTE FDAV KRECSIC
Sbjct: 541 DTSDNLRWKNACGKNGILAQTFKSRITSEGLRREYLTTASQVREVTEIFDAVRKRECSIC 600

Query: 601 LYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAV 660
           LYDLH SAAGCSCS DRYSCLNHAKQLCSCAWG +FF+VR+KMGSLNLLLEALEGKLSAV
Sbjct: 601 LYDLHLSAAGCSCSADRYSCLNHAKQLCSCAWGDKFFVVRYKMGSLNLLLEALEGKLSAV 660

Query: 661 YKWAKENLGLAVHSYKNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKARL 720
           YKWAKENLGLAVHSYKNSS QS P DSP+SSK  QSED+E+ N VN+SI RIKAEIKARL
Sbjct: 661 YKWAKENLGLAVHSYKNSSLQSHPPDSPESSKSYQSEDAETPNAVNNSILRIKAEIKARL 720

Query: 721 LQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEKTASKLQPVSSNELKGKENTST 780
           LQSKTLK+ KE E VIE +DAVKDNG LTNS S IPA+K  SKLQPV SNEL GKE+ ST
Sbjct: 721 LQSKTLKHMKETENVIEPKDAVKDNGILTNSCSSIPADKAVSKLQPVCSNELNGKEDRST 780

Query: 781 PAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDDCWSDS 821
           PAVVLNER DGL+FSLNL+SSE LSESS SSFSESD  WSDS
Sbjct: 781 PAVVLNERKDGLMFSLNLESSEILSESSTSSFSESDGYWSDS 822

BLAST of Lag0001991 vs. NCBI nr
Match: XP_023004812.1 (putative lysine-specific demethylase JMJ16 [Cucurbita maxima] >XP_023004814.1 putative lysine-specific demethylase JMJ16 [Cucurbita maxima])

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 674/817 (82.50%), Postives = 725/817 (88.74%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           MDEDCTR+SLNQGVENLL PPGF SRRSFRLRRVE NE+D SI+T+K +I TLSR   I+
Sbjct: 1   MDEDCTRSSLNQGVENLLAPPGFTSRRSFRLRRVELNENDGSIQTKKTKIDTLSRKIAID 60

Query: 61  M-EAACRKRPWILFNQN-KDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
           + EAACR+RPW+LFNQN +DSLEFE M+ D++ PPQSDLPKGVAHGCPECSNCLKVTARW
Sbjct: 61  LVEAACRQRPWVLFNQNTEDSLEFESMQHDVDPPPQSDLPKGVAHGCPECSNCLKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
           HPE ART+IL++APVFYP+EEEF+DTL +IERIR RAE  GICRIVPPPSWLPPC LKE 
Sbjct: 121 HPEDARTNILDEAPVFYPSEEEFSDTLSFIERIRSRAESSGICRIVPPPSWLPPCLLKET 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWE+SPFLA  QRIDG QK+YACG+  +HS NI  + RRLEYEC NRCL D DESCG D
Sbjct: 181 EIWEDSPFLAQCQRIDGIQKDYACGRLAKHSENITKKRRRLEYECGNRCLKDSDESCGSD 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            KGP SELG+E TLKAFKSYADDFKSQYFS GNKVTNTE  S +LQEQWEP+VDQ+EGEY
Sbjct: 241 KKGPYSELGQEFTLKAFKSYADDFKSQYFSSGNKVTNTETKSSMLQEQWEPLVDQIEGEY 300

Query: 301 RRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSL 360
           R IVENPTEQIEVL G SLGS SLGSGFP S S  IEPGH DHMDSGWNLNNL RLPGSL
Sbjct: 301 RHIVENPTEQIEVLYGDSLGSPSLGSGFPSSPSSPIEPGHVDHMDSGWNLNNLPRLPGSL 360

Query: 361 LSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFE 420
           LSFDSFKTCS+LS RV+VGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRY DKFE
Sbjct: 361 LSFDSFKTCSILSSRVHVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYIDKFE 420

Query: 421 VAMKSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNC 480
           VAMKSLPE F+EQK      VSQPSIATLKREGIPIYRCIQNPGEFVLV PG CHSGFNC
Sbjct: 421 VAMKSLPETFVEQKRSHRGMVSQPSIATLKREGIPIYRCIQNPGEFVLVLPGTCHSGFNC 480

Query: 481 GFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRK 540
           GFSV+EEANFAPLDWLP+GYNAMELYSVERRKTL+SFDRLLLGA +E VKAQWELSLCRK
Sbjct: 481 GFSVTEEANFAPLDWLPYGYNAMELYSVERRKTLVSFDRLLLGAVIETVKAQWELSLCRK 540

Query: 541 ETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSIC 600
           +T DNLRWKNACGK+GILAQTFKSRI SEGLRREYLTTASQ+REVTE FDAV KRECSIC
Sbjct: 541 DTSDNLRWKNACGKNGILAQTFKSRITSEGLRREYLTTASQVREVTEIFDAVRKRECSIC 600

Query: 601 LYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAV 660
           LYDLH SAAGCSCS DRYSCLNHAKQLCSCAWG +FF+VR+KMGSLNLLLEALEGKLSAV
Sbjct: 601 LYDLHLSAAGCSCSADRYSCLNHAKQLCSCAWGDKFFVVRYKMGSLNLLLEALEGKLSAV 660

Query: 661 YKWAKENLGLAVHSYKNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKARL 720
           YKWAKENLGLAVHSYKNSS QS P DSPQSSK  QSED+E+ N VN+SI RIKAEIKARL
Sbjct: 661 YKWAKENLGLAVHSYKNSSVQSHPPDSPQSSKSHQSEDAETPNAVNNSILRIKAEIKARL 720

Query: 721 LQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEKTASKLQPVSSNELKGKENTST 780
           LQSKTLK+ KE E VIE +DAVKDNG LTNS S   AEKTASKLQP+SSNEL+GKE+ ST
Sbjct: 721 LQSKTLKHMKETENVIEPKDAVKDNGILTNSYSS-TAEKTASKLQPMSSNELEGKEDRST 780

Query: 781 PAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDD 816
           PAVVLNER DGL+FSLNL+SSE LSESS SSFSESDD
Sbjct: 781 PAVVLNERKDGLMFSLNLESSEILSESSTSSFSESDD 816

BLAST of Lag0001991 vs. NCBI nr
Match: XP_038898268.1 (putative lysine-specific demethylase JMJ16 isoform X2 [Benincasa hispida])

HSP 1 Score: 1347.8 bits (3487), Expect = 0.0e+00
Identity = 681/823 (82.75%), Postives = 722/823 (87.73%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           MDEDCT NSLNQGVENLL  PGFISRRSFRLRRVEQN +DDSIKT+K   GTLS  +D E
Sbjct: 1   MDEDCTTNSLNQGVENLLASPGFISRRSFRLRRVEQNANDDSIKTKKTGKGTLSGTSDGE 60

Query: 61  -MEAACRKRPWILFNQNK-DSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
            M AACR+RPWILF+QNK DSLEFEPME D+N+PPQSDLPKGVAHGCPECSNC KVTARW
Sbjct: 61  MMAAACRQRPWILFDQNKEDSLEFEPMEHDVNIPPQSDLPKGVAHGCPECSNCQKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
            P+ AR+DILE+A VFYPTEEEFADTLHYIERIR RAE CGICRIVPPPSWLPPC LKEK
Sbjct: 121 RPDDARSDILEEAAVFYPTEEEFADTLHYIERIRSRAESCGICRIVPPPSWLPPCLLKEK 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWENSPFLAHYQRIDGFQK  A  QF  HSG++KN+ RRLEYE  NRCLMDPDESCG D
Sbjct: 181 EIWENSPFLAHYQRIDGFQKTCARDQFSNHSGDVKNKRRRLEYEYGNRCLMDPDESCGSD 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            KGPNSELGRE TLKAFKSYADDFKSQYFS GNKVTNTE  S +LQEQWEP+VDQVEGEY
Sbjct: 241 KKGPNSELGREFTLKAFKSYADDFKSQYFSSGNKVTNTETKSSMLQEQWEPLVDQVEGEY 300

Query: 301 RRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSL 360
           RRI+ENPTEQIEVLCG SLG+ SLGSGFP SSS   E GH DHMDSGWNLNNL RLPGSL
Sbjct: 301 RRILENPTEQIEVLCGDSLGTLSLGSGFPSSSSLWTETGHVDHMDSGWNLNNLPRLPGSL 360

Query: 361 LSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFE 420
           LS DSF+TCS+L P+V VGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRY DKF+
Sbjct: 361 LSLDSFRTCSILLPQVCVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYIDKFD 420

Query: 421 VAMKSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNC 480
           VAMKSLPE F+EQK      VSQPSIATLKREGIPIYRCIQNPGEFVLVFPG CHSGFNC
Sbjct: 421 VAMKSLPEFFVEQKRSHRGMVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGACHSGFNC 480

Query: 481 GFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRK 540
           GFSV+EEANFAPLDWLP GY + E YSVERRKTLISFDRLLLGAA+EAVKAQWELSLCRK
Sbjct: 481 GFSVTEEANFAPLDWLPHGYISTERYSVERRKTLISFDRLLLGAAIEAVKAQWELSLCRK 540

Query: 541 -ETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSI 600
            ETKDNLRWKNACGK GILAQT KSRI+SE LRREYLTTASQ REVTES DAV K +CSI
Sbjct: 541 EETKDNLRWKNACGKHGILAQTIKSRIRSESLRREYLTTASQTREVTESSDAVRKYKCSI 600

Query: 601 CLYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSA 660
           CL DLH SAAGCSCS DRYSCL HAKQLCSCAW  +FF+VR+KM +LNLLLEALEGKLSA
Sbjct: 601 CLCDLHLSAAGCSCSADRYSCLIHAKQLCSCAWSNKFFVVRYKMDNLNLLLEALEGKLSA 660

Query: 661 VYKWAKENLGLAVHSYKNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKAR 720
           VYKWAKENLGLAVHSYKNSS QS PAD+PQSS+G QSED+ SSN VN+SINRIKAEIKAR
Sbjct: 661 VYKWAKENLGLAVHSYKNSSLQSQPADTPQSSEGHQSEDAGSSNAVNNSINRIKAEIKAR 720

Query: 721 LLQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEKTASKLQPVSSNELKGKENTS 780
           LLQ+KTLK  K +  V ES DAVKDNG +TN+DSRI AEKTASKLQPV SNELKGKE TS
Sbjct: 721 LLQAKTLKNMKGSGGVTESVDAVKDNGIVTNTDSRISAEKTASKLQPVCSNELKGKERTS 780

Query: 781 TPAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDDCWSDS 821
           T AVVLN RGD LIFSLNL+SSE L ESSVSS  ESD+ WSDS
Sbjct: 781 TQAVVLNGRGDDLIFSLNLESSEILPESSVSSSLESDNDWSDS 823

BLAST of Lag0001991 vs. ExPASy Swiss-Prot
Match: C0SUT9 (Putative lysine-specific demethylase JMJ16 OS=Arabidopsis thaliana OX=3702 GN=JMJ16 PE=2 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 8.5e-175
Identity = 342/768 (44.53%), Postives = 458/768 (59.64%), Query Frame = 0

Query: 20  PPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIEMEA-------ACRKRPWIL 79
           PPGF S  +F L+RV    + D  KT   E  + +    +E+E+       A R+RPWI 
Sbjct: 21  PPGFESYATFTLKRVVPATTSDKAKTPAIESVSATEQAKMEVESDEAKAARALRRRPWIN 80

Query: 80  FN---------QNKDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARWHPEAA 139
            +          N D+   +  +++ ++ P   LPKGV  GC EC +C KVTARWHP+ A
Sbjct: 81  HSGCDDDGDCAANNDNAASQNPDQNCDVKPA--LPKGVVRGCEECKDCQKVTARWHPDEA 140

Query: 140 RTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEKEIWEN 199
           R   LEDAPVFYP+EEEF DTL+YI +IRP AE  GICRIVPPPSW PPC LKEK++WE 
Sbjct: 141 RRPDLEDAPVFYPSEEEFEDTLNYIAKIRPEAEKYGICRIVPPPSWKPPCPLKEKQVWEG 200

Query: 200 SPFLAHYQRIDGFQKNYA---CGQFPEHSGNIKNRWRRLEYECVNRCLMDP-DESCGVDN 259
           S F    QR+D  Q   +     + P      K +  ++  + V   + DP   S G++ 
Sbjct: 201 SKFTTRVQRVDKLQNRSSMKKISKLPNQMRKKKRKCMKMGMDSVTNGMGDPCSASTGMNE 260

Query: 260 -KGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 319
            +    E G   TLK F+ YAD+FK+QYF      T+ +       + WEP ++ VEGEY
Sbjct: 261 LETFGFEPGPGFTLKDFQKYADEFKAQYFKKSETSTDDKCKVDNSIDCWEPALEDVEGEY 320

Query: 320 RRIVENPTEQIEVLCGGSLGSHSLGSGFPP-SSSPLIEPGHADHMDSGWNLNNLARLPGS 379
            RIV+  TE+IEVL G  L +   GSGFP  SSS         +  SGWNLNN  RLPGS
Sbjct: 321 WRIVDKATEEIEVLYGADLETGVFGSGFPKISSSHNASSSEDKYAKSGWNLNNFPRLPGS 380

Query: 380 LLSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKF 439
           LL ++      VL P +Y+GMCFS+ CW VE+HHL  L Y+H GAPK+WYG+ G+   K 
Sbjct: 381 LLKYEGSDISGVLVPWLYIGMCFSSFCWHVEDHHLYSLNYMHWGAPKLWYGVGGKDAVKL 440

Query: 440 EVAM-KSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGF 499
           E AM K LP++F EQ   L   V+Q S + LK  G+P++RC+Q+ GEFVL FP   H+GF
Sbjct: 441 EEAMRKHLPDLFEEQPDLLHKLVTQLSPSKLKTAGVPVHRCVQHAGEFVLTFPRAYHAGF 500

Query: 500 NCGFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLC 559
           N GF+ +E  N AP+DWLP G  A+ELY  + RKT IS D+LLLGAA E VKA WEL+L 
Sbjct: 501 NSGFNCAEAVNVAPVDWLPHGQIAIELYCQQGRKTSISHDKLLLGAAREVVKADWELNLL 560

Query: 560 RKETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECS 619
           RK T DNLRWK    KDGILA+T K+RI  E  RRE+L  +S   ++  +FDA  +REC 
Sbjct: 561 RKNTVDNLRWKAFSAKDGILAKTLKARIDMERTRREFLCNSSLALKMHSNFDATNERECC 620

Query: 620 ICLYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLS 679
           IC +DLH SAAGC CS ++YSCL H K+LCSC W  ++FL R+ +  LN+L+EA+EGKLS
Sbjct: 621 ICFFDLHLSAAGCRCSPEKYSCLTHVKELCSCPWVTKYFLFRYDIDELNVLVEAVEGKLS 680

Query: 680 AVYKWAKENLGLAVHSYKNSSPQSLPAD-------SPQSS--KGQQSEDSESSNTVNSSI 739
           +VY+WA+++LGLA+ +  + S   +  +       +PQ++   G+  +   +S  V+  +
Sbjct: 681 SVYRWARQDLGLALSTDVSGSKMEIDEEGKVHKDPTPQTTALSGKDLQLKVTSKEVSKEL 740

Query: 740 NRIKAEIKARLLQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPA 756
            +        LL    LK K+E          VK+     +SD  + A
Sbjct: 741 EKTSKLSHVNLL----LKEKEEQITSSHCMKPVKEETVCDSSDPNVSA 782

BLAST of Lag0001991 vs. ExPASy Swiss-Prot
Match: Q53WJ1 (Lysine-specific demethylase JMJ703 OS=Oryza sativa subsp. japonica OX=39947 GN=JMJ703 PE=1 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 5.0e-159
Identity = 312/705 (44.26%), Postives = 419/705 (59.43%), Query Frame = 0

Query: 66  RKRPWILFNQ----NKDSLEFEPMERDM-NLPPQSDLPKGVAHGCPECSNCLKVTARWHP 125
           R RP I +++    + +  + E +E+D+ +   +  LPKGV  GC ECS+C KV A+W+P
Sbjct: 84  RNRPPIDYSRFEHISDEDSDVEIVEKDVSSTRRRQQLPKGVLRGCAECSDCQKVIAKWNP 143

Query: 126 EAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEKEI 185
             AR  +L++APVFYPTEEEF DTL YIE IRP AE  GICRIVPP SW PPC LK+K I
Sbjct: 144 AGARRPVLDEAPVFYPTEEEFEDTLKYIESIRPMAEPYGICRIVPPSSWKPPCLLKDKSI 203

Query: 186 WENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVDNK 245
           WE S F    Q++D  Q N    +     G +K   R+L     N              +
Sbjct: 204 WEGSKFSTRVQKVDKLQ-NRKSSKKGRRGGMMKR--RKLAESEENSATAHTQTGMQQSPE 263

Query: 246 GPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEYRR 305
               E G E TL+ F+ YADDF  QYF    K T+ +           P V+ +EGEY R
Sbjct: 264 RFGFEPGPEFTLQTFQKYADDFSKQYF---RKDTSMDS---------VPSVEDIEGEYWR 323

Query: 306 IVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSLLS 365
           IVE PTE+IEV+ G  L + + GSGFP  S          +  SGWNLNNL RL GS+LS
Sbjct: 324 IVEVPTEEIEVIYGADLETGTFGSGFPKLSPETKSDAEDKYAQSGWNLNNLPRLQGSVLS 383

Query: 366 FDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFEVA 425
           F+      VL P VYVGMCFS+ CW VE+HHL  L Y+H GAPK+WYG+PG+     E A
Sbjct: 384 FEGGDISGVLVPWVYVGMCFSSFCWHVEDHHLYSLNYMHWGAPKLWYGVPGKDAVNLESA 443

Query: 426 M-KSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNCG 485
           M K LPE+F EQ   L   V+Q S + LK EG+ +YRC+Q+ GEFVL FP   H+GFNCG
Sbjct: 444 MRKHLPELFEEQPDLLHNLVTQFSPSLLKSEGVHVYRCVQHEGEFVLTFPRAYHAGFNCG 503

Query: 486 FSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRKE 545
           F+ +E  N AP+DWLP G+NA+ELY  + RK  IS D+LLLGAA EA++AQW++   ++ 
Sbjct: 504 FNCAEAVNVAPIDWLPIGHNAVELYREQARKITISHDKLLLGAAREAIRAQWDILFLKRN 563

Query: 546 TKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSICL 605
           T DN+RWK+ CG D  + +  K+RI++E ++R+ L   +Q R++   FD++  REC++C 
Sbjct: 564 TADNMRWKSICGADSTIFKALKARIETELVQRKTLGVPAQSRKMDAEFDSI-DRECALCY 623

Query: 606 YDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAVY 665
           YDLH SA+GC C  ++Y+CL HAKQLCSC W  RFFL R+ +  LN+L +AL GKLSA++
Sbjct: 624 YDLHLSASGCPCCPEKYACLVHAKQLCSCDWDKRFFLFRYDVNELNILADALGGKLSAIH 683

Query: 666 KWAKENLGLAVHS------YKNSSPQSLPADSPQSSKGQQSE-----DSESSNTVNSSIN 725
           +W   +LGL++ S       ++S       D P+ S   Q+       S +SN      N
Sbjct: 684 RWGVSDLGLSLSSCVKREKVQDSKTVRRLTDGPRRSYMSQASAVSLVSSSTSNEQKDEGN 743

Query: 726 RIK--AEIKARLLQSKTLKYKKENEKVIESEDAVKDNGTLTNSDS 752
           +I   A  +   +     + K EN   ++      +    TNSDS
Sbjct: 744 KIMKIASPQTNNVCPSVEQRKSENISPLKEPCVRNELSCTTNSDS 772

BLAST of Lag0001991 vs. ExPASy Swiss-Prot
Match: Q8GUI6 (Probable lysine-specific demethylase JMJ14 OS=Arabidopsis thaliana OX=3702 GN=JMJ14 PE=1 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 6.0e-136
Identity = 268/637 (42.07%), Postives = 371/637 (58.24%), Query Frame = 0

Query: 113 KVTARWHPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPP 172
           K+TARW+P  A   +++DAP+FYPT E+F D L YIE++R +AE  GICRIVPP +W PP
Sbjct: 38  KITARWNPSEACRPLVDDAPIFYPTNEDFDDPLGYIEKLRSKAESYGICRIVPPVAWRPP 97

Query: 173 CFLKEKEIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPD 232
           C LKEK+IWENS F    Q ID  Q      +  +     + R  ++ Y    R     D
Sbjct: 98  CPLKEKKIWENSKFPTRIQFIDLLQNREPIKKSTKTKKRKRRRISKIGYTRRKR-----D 157

Query: 233 ESCGVDNKGPNS-------ELGREVTLKAFKSYADDFKSQYF-SCGNKVTNTEENSPLLQ 292
             C   + G +        + G + TL+ F+ Y + FK  YF S  +  +   EN     
Sbjct: 158 SGCDTASSGSSDSEGKFGFQTGPDFTLEEFQKYDEYFKECYFQSEDHPGSKASEN----- 217

Query: 293 EQWEPMVDQVEGEYRRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHAD-HMD 352
           ++++P V  +EGEY RIVE  T+++EV  G  L +   GSGF P   P      AD +  
Sbjct: 218 KKFKPKVKDLEGEYWRIVEQATDEVEVYYGADLETKKFGSGF-PKYKPGYPISEADQYSQ 277

Query: 353 SGWNLNNLARLPGSLLSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAP 412
            GWNLNNL+RLPGS+L+F+S     V+ P +YVGMCFST CW VE+HHL  + YLH G P
Sbjct: 278 CGWNLNNLSRLPGSVLAFESCDISGVIVPWLYVGMCFSTFCWHVEDHHLYSMNYLHTGDP 337

Query: 413 KIWYGIPGRYFDKFEVAMKS-LPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPG 472
           K+WYGIPG + + FE  MK  LP++F EQ   L   V+Q S   LK EG+P+YR +Q  G
Sbjct: 338 KVWYGIPGNHAESFENVMKKRLPDLFEEQPDLLHQLVTQLSPRILKEEGVPVYRAVQRSG 397

Query: 473 EFVLVFPGVCHSGFNCGFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGA 532
           EF+L FP   HSGFNCGF+ +E  N AP+DWL  G NA+E YS +RRK+ +S D+LLLGA
Sbjct: 398 EFILTFPKAYHSGFNCGFNCAEAVNVAPVDWLVHGQNAVEGYSKQRRKSSLSHDKLLLGA 457

Query: 533 AVEAVKAQWELSLCRKETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMRE 592
           A+EA    WELSL +K+T    RWK  C +DG+L +  K R++ E  R  +L     +R+
Sbjct: 458 AMEATYCLWELSLSKKKTPVIARWKRVCSEDGLLTKAVKKRVQMEEERLNHLQDGFSLRK 517

Query: 593 VTESFDAVGKRECSICLYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMG 652
           +   FD   +REC +C YDLH SA+ C CS +R++CL HAK LCSC    R+ L+RH + 
Sbjct: 518 MEGDFDNKRERECFLCFYDLHMSASSCKCSPNRFACLIHAKDLCSCESKDRYILIRHTLD 577

Query: 653 SLNLLLEALEGKLSAVYKWA--------KENLGLAVHSYKNSSPQSLPADSPQSSKGQQS 712
            L  L+ ALEG L A+  WA         ++     ++Y  S+P      S  SSK QQ 
Sbjct: 578 ELWALVRALEGDLDAIDLWASKCRDQYPSQHPRAREYAYLKSAP---CIKSRGSSKVQQR 637

Query: 713 EDSESSNTVNSSINRIKAEIKARLLQSKTLKYKKENE 732
           E         +++  +   +++ L  +K ++ K++ +
Sbjct: 638 E--------QNNLQLVSERLQSDLTSNKEVQLKQDGD 652

BLAST of Lag0001991 vs. ExPASy Swiss-Prot
Match: F4I6G4 (Lysine-specific demethylase JMJ18 OS=Arabidopsis thaliana OX=3702 GN=JMJ18 PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 6.6e-135
Identity = 273/626 (43.61%), Postives = 356/626 (56.87%), Query Frame = 0

Query: 80  LEFEPMERDM-------NLPPQSDLPKGVAHGCPECSNCLKVTARWHPEAARTDILEDAP 139
           +E  P+E ++       N PP  D  K      P      KV ARW P+ A+  I+ DAP
Sbjct: 1   MENPPLESEIKEDMSLKNHPPDKDKDKDTIMEQPSSPRHRKVVARWLPDEAQRPIINDAP 60

Query: 140 VFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEKEIWENSPFLAHYQR 199
           VF P+ EEF D L YIE+IRP AE  GICRI+PP +W PPC LKEK IWE + F    Q 
Sbjct: 61  VFTPSLEEFVDPLAYIEKIRPLAEPYGICRIIPPSTWKPPCRLKEKSIWEQTKFPTRIQT 120

Query: 200 IDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESC----GVDNKGPNSELGR 259
           +D  Q      + P+     + R  R+      R    P ES       +  G NS  G 
Sbjct: 121 VDLLQNREPMKKKPKSRKRKRRRNSRMG-SSKRRSGSSPAESTSSPEAEEKFGFNS--GS 180

Query: 260 EVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEYRRIVENPTEQ 319
           + TL  F+ YA  FK  YF         +++S     +W P VD +EGEY RIVE PT++
Sbjct: 181 DFTLDEFEKYALHFKDSYFE--------KKDSGGDIVKWTPSVDDIEGEYWRIVEQPTDE 240

Query: 320 IEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSLLSFDSFKTCS 379
           +EV  G  L +  LGSGF   +          +  SGWNLNNL RLPGS+LSF+      
Sbjct: 241 VEVYYGADLENGVLGSGFYKRAEKFTGSDMEQYTLSGWNLNNLPRLPGSVLSFEDCDISG 300

Query: 380 VLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFEVAM-KSLPEI 439
           VL P +YVGMCFS+ CW VE+HHL  L Y H G PK+WYG+PG      E AM K LP++
Sbjct: 301 VLVPWLYVGMCFSSFCWHVEDHHLYSLNYHHFGEPKVWYGVPGSNATALEKAMRKHLPDL 360

Query: 440 FLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNCGFSVSEEAN 499
           F EQ   L   V+Q S + LK EG+  YR +QN GE+VL FP   H+GFNCGF+ +E  N
Sbjct: 361 FEEQPDLLHGLVTQFSPSILKDEGVQAYRVVQNSGEYVLTFPRAYHAGFNCGFNCAEAVN 420

Query: 500 FAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCR-KETKDNLRW 559
            AP+DWL  G NA+ELYS E RKT +S D+LLLGAA EAVKA WELS    KE   NLRW
Sbjct: 421 VAPVDWLAHGQNAVELYSKETRKTSLSHDKLLLGAAYEAVKALWELSASEGKENTTNLRW 480

Query: 560 KNACGKDGILAQTFKSRIKSEGLRREYL-TTASQMREVTESFDAVGKRECSICLYDLHFS 619
           K+ CGK+G L    ++R++ E  R   L   +S ++++ + FD+  +REC  C YDLH S
Sbjct: 481 KSFCGKNGTLTNAIQARLQMEEGRITALGRDSSSLKKMEKDFDSNCERECFSCFYDLHLS 540

Query: 620 AAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAVYKWAKEN 679
           A+GC CS + Y+CL HA  LCSC     F L+R+ M  L+ L+ ALEG+   +  WA + 
Sbjct: 541 ASGCKCSPEEYACLKHADDLCSCDVKDGFILLRYTMDELSSLVRALEGESDDLKIWASKV 600

Query: 680 LGLAVHSYKNSSPQSLPADSPQSSKG 692
           LG+       +   S+ ++  +  +G
Sbjct: 601 LGIEHSDEDQTKTSSVISEEKKLKEG 615

BLAST of Lag0001991 vs. ExPASy Swiss-Prot
Match: Q8L7T6 (Probable inactive lysine-specific demethylase JMJ19 OS=Arabidopsis thaliana OX=3702 GN=JMJ19 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-129
Identity = 289/752 (38.43%), Postives = 416/752 (55.32%), Query Frame = 0

Query: 14  VENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIEMEAACRKRPWILF 73
           ++ +  PPGF+S+ SF LR V +++      +R+ +                + RPWI+ 
Sbjct: 15  MDTISAPPGFVSQTSFVLRNVPRDKESPRSVSRQEQTTGFGTDDKDSCNMFLKSRPWIVH 74

Query: 74  NQNKDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARWHPEAARTDILEDAPV 133
                S E         L P+                  +V  R   + + T +LE+APV
Sbjct: 75  GHTIPSSE--------ALRPKK----------------TEVRRRRPLKVSETKVLEEAPV 134

Query: 134 FYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEKEIWENSPFLAHYQRI 193
           F PTEEEF DTL YI  +R RAE  GIC +VPPPSW PPC LKEK+IWE S F    Q +
Sbjct: 135 FNPTEEEFRDTLSYISSLRDRAEPYGICCVVPPPSWKPPCLLKEKQIWEASTFFPQVQ-L 194

Query: 194 DGFQKNYACGQFPEHSGNIKNRWRRLEYEC-VNRCLMDPDESCGVDNKGPNSELGREVTL 253
            G Q               +NR  + E +   N    +  + C V+ +GP        TL
Sbjct: 195 FGIQ--------------TENRKIKKEVDADSNDAASEGVQLCRVE-RGPG------YTL 254

Query: 254 KAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEYRRIVENPTEQIEVL 313
           K+FK++AD +K  +F   ++V  +E +SP L+   E +V  +E EYR+IVE+P  +I VL
Sbjct: 255 KSFKNFADTYKKSHFGMKDEVLGSENSSPSLKPN-ELIVADIEKEYRQIVESPLIEIGVL 314

Query: 314 CGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSLLSFDSFKTCSVLSP 373
            G  L + + GSGFP S+     P  +    SGWNLN+ A+LPGSLLS +  +  SV  P
Sbjct: 315 YGNDLDTATFGSGFPLSA-----PSESSKYSSGWNLNSTAKLPGSLLSLEDCE--SVCVP 374

Query: 374 RVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFEVAMKS-LPEIFLEQ 433
           R+ VGMC S+  W+ E+  L  LCYLH+GAP++WY + G +  KF+ AMKS + E+  EQ
Sbjct: 375 RLSVGMCLSSQFWKSEKERLYSLCYLHVGAPRVWYSVAGCHRSKFKAAMKSFILEMSGEQ 434

Query: 434 KSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNCGFSVSEEANFAPL 493
                  V   S   L  EGIP+ RC+Q+PG++V++FPG  +S F+CGF+  E+ANFAPL
Sbjct: 435 PKKSHNPVMMMSPYQLSVEGIPVTRCVQHPGQYVIIFPGSYYSAFDCGFNCLEKANFAPL 494

Query: 494 DWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRKETKDNLRWKNACG 553
           DWLP G  A+++     + +LIS+D+LL  AA EAVK   E  L +K T    RW ++CG
Sbjct: 495 DWLPHGDIAVQVNQEMSKTSLISYDKLLFSAAREAVKCLKEYGLSKKNTACYTRWNDSCG 554

Query: 554 KDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSICLYDLHFSAAGCSC 613
            DG+ +   KSRIK E  RRE+L ++ + + + +S+DAV KREC +CL DL+ SA  CSC
Sbjct: 555 TDGLFSNIIKSRIKLEKNRREFLISSLESQRMDKSYDAVNKRECCVCLGDLYLSAVNCSC 614

Query: 614 STDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEG-KLSAVYKWAKENLGLAV 673
           S +RYSCLNH ++LC+C    + FL R+ M  LNLL+EALEG KLS++++WA    G+  
Sbjct: 615 SANRYSCLNHMRKLCACPCDRKSFLYRYTMDELNLLVEALEGKKLSSMFRWA----GI-- 674

Query: 674 HSYKNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSI---NRIKAEIKARLLQSKTLKYK 733
              K  +  +  +  P+  KG+++++    N     +    + +  +KAR L +  L  K
Sbjct: 675 -DQKFCASPATTSSKPEEDKGKETDEVTPCNITRKDVAAGTKDQTRVKARSL-ADILNVK 704

Query: 734 KENEKVIESEDAV--KDNGTLTNSDSRIPAEK 758
             N    E+ ++   K N    N  S   A K
Sbjct: 735 DGNNDAKETLESCSKKSNRPCDNDSSEANAPK 704

BLAST of Lag0001991 vs. ExPASy TrEMBL
Match: A0A6J1DQR3 (putative lysine-specific demethylase JMJ16 OS=Momordica charantia OX=3673 GN=LOC111023467 PE=4 SV=1)

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 681/827 (82.35%), Postives = 741/827 (89.60%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           MDEDC+RNSLNQGVENLL PPGFISRRSFRLRRVEQNE+DDS K ++ EI  LS+   IE
Sbjct: 1   MDEDCSRNSLNQGVENLLAPPGFISRRSFRLRRVEQNENDDSSKIKQTEISALSQTIGIE 60

Query: 61  -MEAACRKRPWILFNQ-NKDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
            MEAACR+RPWILF+Q N+DSLEFE ME DMNLPPQSDLPKGVAHGCPECSNC+KVTARW
Sbjct: 61  MMEAACRQRPWILFDQKNEDSLEFESMENDMNLPPQSDLPKGVAHGCPECSNCVKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
           HPE ARTD LE+APVFYPTEEEFADTL YIERIRPRAE CGICRIVPPPSWLPPC LKEK
Sbjct: 121 HPEDARTDSLEEAPVFYPTEEEFADTLQYIERIRPRAESCGICRIVPPPSWLPPCLLKEK 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWENSPFLAHYQRIDGFQKN+ACG+FP+H+ N+KN+ RR+EYEC NRC MDPDESCG+D
Sbjct: 181 EIWENSPFLAHYQRIDGFQKNFACGRFPKHNDNVKNKRRRIEYECGNRCFMDPDESCGID 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            KGPNS+LGRE TLKAFKSYADDFKSQYFS GNKVT+TE NS  +QEQWEP+VDQVE EY
Sbjct: 241 KKGPNSKLGREFTLKAFKSYADDFKSQYFSSGNKVTDTETNSSTVQEQWEPLVDQVESEY 300

Query: 301 RRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSL 360
           RRIVENPTEQIEVLCG SL S SLGSGFP SSSPLIEPGHADHM+SGWNLNNL RLPGSL
Sbjct: 301 RRIVENPTEQIEVLCGHSLDSPSLGSGFPASSSPLIEPGHADHMESGWNLNNLRRLPGSL 360

Query: 361 LSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFE 420
           LSFDSFKTCS+L P+VYVGMCFS+ CWRVEEHHLPLLCYLHLGAPKIWYGIPGRY  +F+
Sbjct: 361 LSFDSFKTCSILLPQVYVGMCFSSTCWRVEEHHLPLLCYLHLGAPKIWYGIPGRYIGQFD 420

Query: 421 VAMKSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNC 480
           VAMK+LPE F+EQK F    VSQPSIA LKREG+P+YRCIQNPGEFVLVFPG CHSGFNC
Sbjct: 421 VAMKNLPEFFVEQKRFHRGMVSQPSIAMLKREGLPVYRCIQNPGEFVLVFPGACHSGFNC 480

Query: 481 GFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRK 540
           GFS++EEANFAPL WLP+GYNA+ELY+++ RKT ISFDRLLL AA+EAVKAQWELSLCRK
Sbjct: 481 GFSITEEANFAPLHWLPYGYNAVELYAIDGRKTSISFDRLLLRAAIEAVKAQWELSLCRK 540

Query: 541 ETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSIC 600
           ETK+NLRWK+ACGK+G+LAQTFKSRIKSEGLRREYLT+ASQM+EV E++DAV KRECSIC
Sbjct: 541 ETKENLRWKDACGKNGVLAQTFKSRIKSEGLRREYLTSASQMQEVNENYDAVCKRECSIC 600

Query: 601 LYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAV 660
           LYDLH SAAGC CS DRYSCLNHAKQLCSCAWG +FF+VR+KMG+LNLLLEALEGKLSAV
Sbjct: 601 LYDLHLSAAGCPCSADRYSCLNHAKQLCSCAWGDKFFVVRYKMGNLNLLLEALEGKLSAV 660

Query: 661 YKWAKENLGLAVHSY--KNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKA 720
           YKW KENLGLAVHSY  KNS PQS  ADS  SSKG QSED+E  N+ N+SI RIKAEIKA
Sbjct: 661 YKWVKENLGLAVHSYITKNSLPQSQTADSSHSSKGDQSEDAELPNSANTSIIRIKAEIKA 720

Query: 721 RLLQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEK--TASKLQPVSSNELKGKE 780
           RLLQS TLK  K+NEKVIESEDAVK NG  +NSDS IP EK  TAS LQP+SSNELKGKE
Sbjct: 721 RLLQSTTLKRMKKNEKVIESEDAVKYNGNPSNSDSGIPLEKAATASMLQPISSNELKGKE 780

Query: 781 NTSTPAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDDCWSDSG 822
             STPAV LNERGDGLIFSLNL+SSE LSESS SSFS+ DDCWSDSG
Sbjct: 781 KPSTPAVALNERGDGLIFSLNLESSEILSESSASSFSDLDDCWSDSG 827

BLAST of Lag0001991 vs. ExPASy TrEMBL
Match: A0A6J1H785 (putative lysine-specific demethylase JMJ16 OS=Cucurbita moschata OX=3662 GN=LOC111461120 PE=4 SV=1)

HSP 1 Score: 1364.0 bits (3529), Expect = 0.0e+00
Identity = 676/822 (82.24%), Postives = 728/822 (88.56%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           MDEDCTR+SLNQG+ENLL PPGF SRRSFRLRRVEQ E+D SI+T+K +I TLSR  DI+
Sbjct: 1   MDEDCTRSSLNQGLENLLAPPGFTSRRSFRLRRVEQKENDGSIQTKKTKIDTLSRKIDID 60

Query: 61  M-EAACRKRPWILFNQ-NKDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
           + EA CR+RPWILFNQ N+DSLEFE M+ D++ PPQSDLPKGVAHGCPECSNCLKVTARW
Sbjct: 61  LVEATCRQRPWILFNQNNEDSLEFESMQHDVDPPPQSDLPKGVAHGCPECSNCLKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
           HPE ART+IL++APVFYP+EEEF+DTL YIERIR RAE  GICRIVPPPSWLPPC LKE 
Sbjct: 121 HPEDARTNILDEAPVFYPSEEEFSDTLSYIERIRSRAESSGICRIVPPPSWLPPCLLKET 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWE+SPFLAH QRIDG QK+YACG+  +HS NI N+ RRLEYEC NRCL D DESCG D
Sbjct: 181 EIWEDSPFLAHCQRIDGIQKDYACGRLAKHSKNITNKRRRLEYECGNRCLKDSDESCGSD 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            KGP SELG+E TLKAFKSYADDFKSQYFS GNKVT+TE  S +LQEQWEP+VDQ+EGEY
Sbjct: 241 KKGPYSELGQEFTLKAFKSYADDFKSQYFSSGNKVTSTETKSSMLQEQWEPLVDQIEGEY 300

Query: 301 RRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSL 360
           R IVENPTEQIEVL G SLGS SLGSGFP S S  IEPGH DHMDSGWNLNNL RLPGSL
Sbjct: 301 RHIVENPTEQIEVLYGDSLGSPSLGSGFPSSPSSPIEPGHVDHMDSGWNLNNLPRLPGSL 360

Query: 361 LSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFE 420
           LSFDSFKTCS+LSPRV+VGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRY DKFE
Sbjct: 361 LSFDSFKTCSILSPRVHVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYIDKFE 420

Query: 421 VAMKSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNC 480
           VAMKSLPE F+EQK      VSQPSIATLKR GIPIYRCIQNPGEFVLV PG CHSGFNC
Sbjct: 421 VAMKSLPETFVEQKRSHRGMVSQPSIATLKRVGIPIYRCIQNPGEFVLVLPGTCHSGFNC 480

Query: 481 GFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRK 540
           GFSV+EEANFAPLDWLP+GYNAMELYSVERRKT++SFDRLLLGA +E VKAQWELSLCRK
Sbjct: 481 GFSVTEEANFAPLDWLPYGYNAMELYSVERRKTVVSFDRLLLGAVIETVKAQWELSLCRK 540

Query: 541 ETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSIC 600
           +T DNLRWKNACGK+GILAQTFKSRI SEGLRREYLTTASQ+REVTE FDAV KRECSIC
Sbjct: 541 DTSDNLRWKNACGKNGILAQTFKSRITSEGLRREYLTTASQVREVTEIFDAVRKRECSIC 600

Query: 601 LYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAV 660
           LYDLH SAAGCSCS DRYSCLNHAKQLCSCAWG +FF+VR+KMGSLNLLLEALEGKLSAV
Sbjct: 601 LYDLHLSAAGCSCSADRYSCLNHAKQLCSCAWGDKFFVVRYKMGSLNLLLEALEGKLSAV 660

Query: 661 YKWAKENLGLAVHSYKNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKARL 720
           YKWAKENLGLAVHSYKNSS QS P DSP+SSK  QSED+E+ N VN+SI RIKAEIKARL
Sbjct: 661 YKWAKENLGLAVHSYKNSSLQSHPPDSPESSKSYQSEDAETPNAVNNSILRIKAEIKARL 720

Query: 721 LQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEKTASKLQPVSSNELKGKENTST 780
           LQSKTLK+ KE E VIE +DAVKDNG LTNS S IPA+K  SKLQPV SNEL GKE+ ST
Sbjct: 721 LQSKTLKHMKETENVIEPKDAVKDNGILTNSCSSIPADKAVSKLQPVCSNELNGKEDRST 780

Query: 781 PAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDDCWSDS 821
           PAVVLNER DGL+FSLNL+SSE LSESS SSFSESD  WSDS
Sbjct: 781 PAVVLNERKDGLMFSLNLESSEILSESSTSSFSESDGYWSDS 822

BLAST of Lag0001991 vs. ExPASy TrEMBL
Match: A0A6J1KXD9 (putative lysine-specific demethylase JMJ16 OS=Cucurbita maxima OX=3661 GN=LOC111498001 PE=4 SV=1)

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 674/817 (82.50%), Postives = 725/817 (88.74%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           MDEDCTR+SLNQGVENLL PPGF SRRSFRLRRVE NE+D SI+T+K +I TLSR   I+
Sbjct: 1   MDEDCTRSSLNQGVENLLAPPGFTSRRSFRLRRVELNENDGSIQTKKTKIDTLSRKIAID 60

Query: 61  M-EAACRKRPWILFNQN-KDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
           + EAACR+RPW+LFNQN +DSLEFE M+ D++ PPQSDLPKGVAHGCPECSNCLKVTARW
Sbjct: 61  LVEAACRQRPWVLFNQNTEDSLEFESMQHDVDPPPQSDLPKGVAHGCPECSNCLKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
           HPE ART+IL++APVFYP+EEEF+DTL +IERIR RAE  GICRIVPPPSWLPPC LKE 
Sbjct: 121 HPEDARTNILDEAPVFYPSEEEFSDTLSFIERIRSRAESSGICRIVPPPSWLPPCLLKET 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWE+SPFLA  QRIDG QK+YACG+  +HS NI  + RRLEYEC NRCL D DESCG D
Sbjct: 181 EIWEDSPFLAQCQRIDGIQKDYACGRLAKHSENITKKRRRLEYECGNRCLKDSDESCGSD 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            KGP SELG+E TLKAFKSYADDFKSQYFS GNKVTNTE  S +LQEQWEP+VDQ+EGEY
Sbjct: 241 KKGPYSELGQEFTLKAFKSYADDFKSQYFSSGNKVTNTETKSSMLQEQWEPLVDQIEGEY 300

Query: 301 RRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSL 360
           R IVENPTEQIEVL G SLGS SLGSGFP S S  IEPGH DHMDSGWNLNNL RLPGSL
Sbjct: 301 RHIVENPTEQIEVLYGDSLGSPSLGSGFPSSPSSPIEPGHVDHMDSGWNLNNLPRLPGSL 360

Query: 361 LSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFE 420
           LSFDSFKTCS+LS RV+VGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRY DKFE
Sbjct: 361 LSFDSFKTCSILSSRVHVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYIDKFE 420

Query: 421 VAMKSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNC 480
           VAMKSLPE F+EQK      VSQPSIATLKREGIPIYRCIQNPGEFVLV PG CHSGFNC
Sbjct: 421 VAMKSLPETFVEQKRSHRGMVSQPSIATLKREGIPIYRCIQNPGEFVLVLPGTCHSGFNC 480

Query: 481 GFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCRK 540
           GFSV+EEANFAPLDWLP+GYNAMELYSVERRKTL+SFDRLLLGA +E VKAQWELSLCRK
Sbjct: 481 GFSVTEEANFAPLDWLPYGYNAMELYSVERRKTLVSFDRLLLGAVIETVKAQWELSLCRK 540

Query: 541 ETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECSIC 600
           +T DNLRWKNACGK+GILAQTFKSRI SEGLRREYLTTASQ+REVTE FDAV KRECSIC
Sbjct: 541 DTSDNLRWKNACGKNGILAQTFKSRITSEGLRREYLTTASQVREVTEIFDAVRKRECSIC 600

Query: 601 LYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAV 660
           LYDLH SAAGCSCS DRYSCLNHAKQLCSCAWG +FF+VR+KMGSLNLLLEALEGKLSAV
Sbjct: 601 LYDLHLSAAGCSCSADRYSCLNHAKQLCSCAWGDKFFVVRYKMGSLNLLLEALEGKLSAV 660

Query: 661 YKWAKENLGLAVHSYKNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKARL 720
           YKWAKENLGLAVHSYKNSS QS P DSPQSSK  QSED+E+ N VN+SI RIKAEIKARL
Sbjct: 661 YKWAKENLGLAVHSYKNSSVQSHPPDSPQSSKSHQSEDAETPNAVNNSILRIKAEIKARL 720

Query: 721 LQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEKTASKLQPVSSNELKGKENTST 780
           LQSKTLK+ KE E VIE +DAVKDNG LTNS S   AEKTASKLQP+SSNEL+GKE+ ST
Sbjct: 721 LQSKTLKHMKETENVIEPKDAVKDNGILTNSYSS-TAEKTASKLQPMSSNELEGKEDRST 780

Query: 781 PAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDD 816
           PAVVLNER DGL+FSLNL+SSE LSESS SSFSESDD
Sbjct: 781 PAVVLNERKDGLMFSLNLESSEILSESSTSSFSESDD 816

BLAST of Lag0001991 vs. ExPASy TrEMBL
Match: A0A0A0K4R8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G373480 PE=4 SV=1)

HSP 1 Score: 1296.2 bits (3353), Expect = 0.0e+00
Identity = 653/824 (79.25%), Postives = 714/824 (86.65%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           MDEDCT +SLNQGVENLL PPGFISRRSFRL++VEQN +DDSIKT+K E GTLS+ +D+E
Sbjct: 1   MDEDCTTSSLNQGVENLLAPPGFISRRSFRLKKVEQNANDDSIKTKKTEKGTLSKTSDVE 60

Query: 61  M-EAACRKRPWILFNQNK-DSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
           M EAACR+RPWILF+QNK DSLEFE  E + ++PPQSDLPKGVA+GCPECSNCLKVTARW
Sbjct: 61  MVEAACRQRPWILFDQNKEDSLEFESTEHE-SIPPQSDLPKGVAYGCPECSNCLKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
            P+ AR+DILE+A VFYPTEEEFADTLHY+ERIR RAE CGICRIVPPPSWLPPC LKEK
Sbjct: 121 RPDDARSDILEEAAVFYPTEEEFADTLHYVERIRSRAESCGICRIVPPPSWLPPCLLKEK 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWENSPFLAHYQRIDGFQK +A  QF  H G++KN+ R+L+YEC NRCLMDPDESC   
Sbjct: 181 EIWENSPFLAHYQRIDGFQKTFARDQFSNHCGDMKNKRRKLDYECGNRCLMDPDESCSY- 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            +G NSE G+E TLK FKSYADDFKSQYF  GNK TNTE  S +L EQWEP+VDQVEGEY
Sbjct: 241 KQGQNSEHGQEFTLKVFKSYADDFKSQYFRSGNKDTNTETKSSMLGEQWEPLVDQVEGEY 300

Query: 301 RRIVENPTEQIEVLCG-GSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGS 360
           RRI+ENPTEQIEVL G  SL SH LGS FP SSSPL EPGHADHMDSGW LNNL RLPGS
Sbjct: 301 RRILENPTEQIEVLYGDSSLSSHLLGSRFPSSSSPLNEPGHADHMDSGWKLNNLPRLPGS 360

Query: 361 LLSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKF 420
           LLS DSF+T S+LSPR+ VGMCFSTA WRVEEHHLPLLCYLHLGAPKIWYGIPGRY DKF
Sbjct: 361 LLSLDSFETSSILSPRLCVGMCFSTAPWRVEEHHLPLLCYLHLGAPKIWYGIPGRYIDKF 420

Query: 421 EVAMKSLPEIFL-EQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGF 480
           +  MKSLPE F+  Q+S   + V+QPSIATLKREGIPIYRCIQNPGEFVLVFPG CHSGF
Sbjct: 421 DEVMKSLPEHFVGRQRSHRGMVVNQPSIATLKREGIPIYRCIQNPGEFVLVFPGACHSGF 480

Query: 481 NCGFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLC 540
           NCGFSV+EEANFAPLDWLP GYNA ELYSVERRKTLISFDRLLLGAA+EAVKAQWELSLC
Sbjct: 481 NCGFSVTEEANFAPLDWLPHGYNATELYSVERRKTLISFDRLLLGAAIEAVKAQWELSLC 540

Query: 541 RKETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECS 600
           R ETKDNLRWK+ACGK GILAQTFKSRI+SE LRREYL TA QMREVT SFD + KRECS
Sbjct: 541 RNETKDNLRWKDACGKHGILAQTFKSRIRSESLRREYLATALQMREVTSSFDDIRKRECS 600

Query: 601 ICLYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLS 660
           ICLYDLH SAAGCSCS DRYSCL HAKQLCSCAWG +FF+VR++M +LNLLL+ALEGKLS
Sbjct: 601 ICLYDLHLSAAGCSCSGDRYSCLVHAKQLCSCAWGNKFFVVRYQMSNLNLLLDALEGKLS 660

Query: 661 AVYKWAKENLGLAVHSYKNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKA 720
           AVYKWAKENLGLAVHSYKNSS QS PAD+ QSS+G QSED+ES +T NSSINRIKAEIKA
Sbjct: 661 AVYKWAKENLGLAVHSYKNSSLQSQPADTRQSSQGSQSEDAESPSTFNSSINRIKAEIKA 720

Query: 721 RLLQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEKTASKLQPVSSNELKGKENT 780
           RLLQ+KTLKY+KE  KV ES D VKDNG + N D R  AE++ SKLQPVS NE+KGKE+T
Sbjct: 721 RLLQAKTLKYRKEIGKVTESVDTVKDNGIVANLDMRTLAEQSVSKLQPVSPNEVKGKEST 780

Query: 781 STPAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDDCWSDS 821
           STPAVVLNERGD LIFSLNL+S  TL ES     SESD+ WSDS
Sbjct: 781 STPAVVLNERGDDLIFSLNLESFATLPES-----SESDEDWSDS 817

BLAST of Lag0001991 vs. ExPASy TrEMBL
Match: A0A5A7TKW1 (Putative lysine-specific demethylase JMJ16 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G005260 PE=4 SV=1)

HSP 1 Score: 1279.6 bits (3310), Expect = 0.0e+00
Identity = 651/825 (78.91%), Postives = 707/825 (85.70%), Query Frame = 0

Query: 1   MDEDCTRNSLNQGVENLLPPPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIE 60
           M EDCT +SLNQGVENLL PPGFISRRSFRLRRVEQN +DDS KT+K E GTLS+ +D++
Sbjct: 1   MGEDCTTSSLNQGVENLLAPPGFISRRSFRLRRVEQNANDDSNKTKKTEKGTLSKTSDVK 60

Query: 61  -MEAACRKRPWILFNQN-KDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARW 120
            MEAACR+RPWILF QN KDSLEFE  E + ++PPQSDLPKGVA+GCPECSNCLKVTARW
Sbjct: 61  MMEAACRQRPWILFGQNKKDSLEFEFTEHE-SIPPQSDLPKGVAYGCPECSNCLKVTARW 120

Query: 121 HPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEK 180
            P+ AR+D LE+A VFYPTEEEFADTL YIERIR RAE CGICRIVPPPSWLP C LKEK
Sbjct: 121 RPDDARSDPLEEAAVFYPTEEEFADTLQYIERIRSRAEPCGICRIVPPPSWLPACLLKEK 180

Query: 181 EIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESCGVD 240
           EIWENSPFLAHYQRIDGFQK +A  QF  H  ++KN+ RRL+  C NRCLMDPDESC   
Sbjct: 181 EIWENSPFLAHYQRIDGFQKTFAHDQFSNHCVDMKNKRRRLDSVCGNRCLMDPDESC--- 240

Query: 241 NKGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 300
            +G NSELGRE TLK FKSYADDFKSQYFS GNK TNTE  SP+L EQWEP+VDQVEGEY
Sbjct: 241 KQGQNSELGREFTLKVFKSYADDFKSQYFSSGNKDTNTETKSPMLGEQWEPLVDQVEGEY 300

Query: 301 RRIVENPTEQIEVLCG-GSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGS 360
           RRI+ENPTEQIEVL G  SL S  LGSGFP SSSPL EPGHADHMDSGW LNNL RLPGS
Sbjct: 301 RRILENPTEQIEVLYGDSSLSSLLLGSGFPSSSSPLNEPGHADHMDSGWKLNNLPRLPGS 360

Query: 361 LLSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKF 420
           LLS DSFKT S+L PR+ VGMCFSTA WRVEEHHLPLLCYLHLGAPKIWYGIPGR  DKF
Sbjct: 361 LLSLDSFKTSSILLPRLCVGMCFSTAPWRVEEHHLPLLCYLHLGAPKIWYGIPGRCIDKF 420

Query: 421 EVAMKSLPEIFLEQKSFLIIQ-VSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGF 480
           +V MKSLPE F+ ++SFLI Q V+QPSI TLK EGIPIYRCIQNPGEFVLVFPG CHSGF
Sbjct: 421 DVVMKSLPENFVGRQSFLITQVVNQPSITTLKSEGIPIYRCIQNPGEFVLVFPGACHSGF 480

Query: 481 NCGFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLC 540
           NCGFSV+EEANFAPLDWLP GYNA ELYSVERRKTLISFDRLLLGAA+EAVKAQWE SLC
Sbjct: 481 NCGFSVTEEANFAPLDWLPHGYNATELYSVERRKTLISFDRLLLGAAIEAVKAQWEFSLC 540

Query: 541 RKETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECS 600
           R ETKDNLRWK+ACGK GILAQTFKSRI+SE LRREYL TA  M E+T SFDAV KRECS
Sbjct: 541 RNETKDNLRWKDACGKYGILAQTFKSRIRSESLRREYLATALHMHEITRSFDAVRKRECS 600

Query: 601 ICLYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLS 660
           ICLYDLH SAAGCSCS DRYSCL HAKQLCSC WG +FF+VR++M +LNLLLEALEGKLS
Sbjct: 601 ICLYDLHLSAAGCSCSGDRYSCLIHAKQLCSCPWGNKFFVVRYQMSNLNLLLEALEGKLS 660

Query: 661 AVYKWAKENLGLAVHSYKNSSPQSLPADSPQSSKGQQSEDSESSNTVNSSINRIKAEIKA 720
           AVYKWAKENLGLAVHSYKN+S QS PAD+PQSS+  +SED ES +T NSSI+RIK+EIKA
Sbjct: 661 AVYKWAKENLGLAVHSYKNTSLQSQPADTPQSSQRSRSEDVESPSTFNSSIDRIKSEIKA 720

Query: 721 RLLQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPAEKTASKLQPVSSNELKGKENT 780
           RLLQ+KTLKY+KE  KV ES D VKDNG + NSD R  AE++ SKLQPVSSNELKGKE+T
Sbjct: 721 RLLQAKTLKYRKETGKVTESADTVKDNGIVANSDMRTLAEQSVSKLQPVSSNELKGKEST 780

Query: 781 STPAVVLNERGDGLIFSLNLQSSETLSESSVSSFSESDDCWSDSG 822
           STPAVVLNERGD LIFSLNL+S  TL ES     SESD+ WSDSG
Sbjct: 781 STPAVVLNERGDDLIFSLNLESLATLHES-----SESDEDWSDSG 816

BLAST of Lag0001991 vs. TAIR 10
Match: AT1G08620.1 (Transcription factor jumonji (jmj) family protein / zinc finger (C5HC2 type) family protein )

HSP 1 Score: 615.5 bits (1586), Expect = 6.0e-176
Identity = 342/768 (44.53%), Postives = 458/768 (59.64%), Query Frame = 0

Query: 20  PPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIEMEA-------ACRKRPWIL 79
           PPGF S  +F L+RV    + D  KT   E  + +    +E+E+       A R+RPWI 
Sbjct: 21  PPGFESYATFTLKRVVPATTSDKAKTPAIESVSATEQAKMEVESDEAKAARALRRRPWIN 80

Query: 80  FN---------QNKDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARWHPEAA 139
            +          N D+   +  +++ ++ P   LPKGV  GC EC +C KVTARWHP+ A
Sbjct: 81  HSGCDDDGDCAANNDNAASQNPDQNCDVKPA--LPKGVVRGCEECKDCQKVTARWHPDEA 140

Query: 140 RTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEKEIWEN 199
           R   LEDAPVFYP+EEEF DTL+YI +IRP AE  GICRIVPPPSW PPC LKEK++WE 
Sbjct: 141 RRPDLEDAPVFYPSEEEFEDTLNYIAKIRPEAEKYGICRIVPPPSWKPPCPLKEKQVWEG 200

Query: 200 SPFLAHYQRIDGFQKNYA---CGQFPEHSGNIKNRWRRLEYECVNRCLMDP-DESCGVDN 259
           S F    QR+D  Q   +     + P      K +  ++  + V   + DP   S G++ 
Sbjct: 201 SKFTTRVQRVDKLQNRSSMKKISKLPNQMRKKKRKCMKMGMDSVTNGMGDPCSASTGMNE 260

Query: 260 -KGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 319
            +    E G   TLK F+ YAD+FK+QYF      T+ +       + WEP ++ VEGEY
Sbjct: 261 LETFGFEPGPGFTLKDFQKYADEFKAQYFKKSETSTDDKCKVDNSIDCWEPALEDVEGEY 320

Query: 320 RRIVENPTEQIEVLCGGSLGSHSLGSGFPP-SSSPLIEPGHADHMDSGWNLNNLARLPGS 379
            RIV+  TE+IEVL G  L +   GSGFP  SSS         +  SGWNLNN  RLPGS
Sbjct: 321 WRIVDKATEEIEVLYGADLETGVFGSGFPKISSSHNASSSEDKYAKSGWNLNNFPRLPGS 380

Query: 380 LLSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKF 439
           LL ++      VL P +Y+GMCFS+ CW VE+HHL  L Y+H GAPK+WYG+ G+   K 
Sbjct: 381 LLKYEGSDISGVLVPWLYIGMCFSSFCWHVEDHHLYSLNYMHWGAPKLWYGVGGKDAVKL 440

Query: 440 EVAM-KSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGF 499
           E AM K LP++F EQ   L   V+Q S + LK  G+P++RC+Q+ GEFVL FP   H+GF
Sbjct: 441 EEAMRKHLPDLFEEQPDLLHKLVTQLSPSKLKTAGVPVHRCVQHAGEFVLTFPRAYHAGF 500

Query: 500 NCGFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLC 559
           N GF+ +E  N AP+DWLP G  A+ELY  + RKT IS D+LLLGAA E VKA WEL+L 
Sbjct: 501 NSGFNCAEAVNVAPVDWLPHGQIAIELYCQQGRKTSISHDKLLLGAAREVVKADWELNLL 560

Query: 560 RKETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECS 619
           RK T DNLRWK    KDGILA+T K+RI  E  RRE+L  +S   ++  +FDA  +REC 
Sbjct: 561 RKNTVDNLRWKAFSAKDGILAKTLKARIDMERTRREFLCNSSLALKMHSNFDATNERECC 620

Query: 620 ICLYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLS 679
           IC +DLH SAAGC CS ++YSCL H K+LCSC W  ++FL R+ +  LN+L+EA+EGKLS
Sbjct: 621 ICFFDLHLSAAGCRCSPEKYSCLTHVKELCSCPWVTKYFLFRYDIDELNVLVEAVEGKLS 680

Query: 680 AVYKWAKENLGLAVHSYKNSSPQSLPAD-------SPQSS--KGQQSEDSESSNTVNSSI 739
           +VY+WA+++LGLA+ +  + S   +  +       +PQ++   G+  +   +S  V+  +
Sbjct: 681 SVYRWARQDLGLALSTDVSGSKMEIDEEGKVHKDPTPQTTALSGKDLQLKVTSKEVSKEL 740

Query: 740 NRIKAEIKARLLQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPA 756
            +        LL    LK K+E          VK+     +SD  + A
Sbjct: 741 EKTSKLSHVNLL----LKEKEEQITSSHCMKPVKEETVCDSSDPNVSA 782

BLAST of Lag0001991 vs. TAIR 10
Match: AT1G08620.2 (Transcription factor jumonji (jmj) family protein / zinc finger (C5HC2 type) family protein )

HSP 1 Score: 615.5 bits (1586), Expect = 6.0e-176
Identity = 342/768 (44.53%), Postives = 458/768 (59.64%), Query Frame = 0

Query: 20  PPGFISRRSFRLRRVEQNESDDSIKTRKAEIGTLSRMTDIEMEA-------ACRKRPWIL 79
           PPGF S  +F L+RV    + D  KT   E  + +    +E+E+       A R+RPWI 
Sbjct: 21  PPGFESYATFTLKRVVPATTSDKAKTPAIESVSATEQAKMEVESDEAKAARALRRRPWIN 80

Query: 80  FN---------QNKDSLEFEPMERDMNLPPQSDLPKGVAHGCPECSNCLKVTARWHPEAA 139
            +          N D+   +  +++ ++ P   LPKGV  GC EC +C KVTARWHP+ A
Sbjct: 81  HSGCDDDGDCAANNDNAASQNPDQNCDVKPA--LPKGVVRGCEECKDCQKVTARWHPDEA 140

Query: 140 RTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEKEIWEN 199
           R   LEDAPVFYP+EEEF DTL+YI +IRP AE  GICRIVPPPSW PPC LKEK++WE 
Sbjct: 141 RRPDLEDAPVFYPSEEEFEDTLNYIAKIRPEAEKYGICRIVPPPSWKPPCPLKEKQVWEG 200

Query: 200 SPFLAHYQRIDGFQKNYA---CGQFPEHSGNIKNRWRRLEYECVNRCLMDP-DESCGVDN 259
           S F    QR+D  Q   +     + P      K +  ++  + V   + DP   S G++ 
Sbjct: 201 SKFTTRVQRVDKLQNRSSMKKISKLPNQMRKKKRKCMKMGMDSVTNGMGDPCSASTGMNE 260

Query: 260 -KGPNSELGREVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEY 319
            +    E G   TLK F+ YAD+FK+QYF      T+ +       + WEP ++ VEGEY
Sbjct: 261 LETFGFEPGPGFTLKDFQKYADEFKAQYFKKSETSTDDKCKVDNSIDCWEPALEDVEGEY 320

Query: 320 RRIVENPTEQIEVLCGGSLGSHSLGSGFPP-SSSPLIEPGHADHMDSGWNLNNLARLPGS 379
            RIV+  TE+IEVL G  L +   GSGFP  SSS         +  SGWNLNN  RLPGS
Sbjct: 321 WRIVDKATEEIEVLYGADLETGVFGSGFPKISSSHNASSSEDKYAKSGWNLNNFPRLPGS 380

Query: 380 LLSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKF 439
           LL ++      VL P +Y+GMCFS+ CW VE+HHL  L Y+H GAPK+WYG+ G+   K 
Sbjct: 381 LLKYEGSDISGVLVPWLYIGMCFSSFCWHVEDHHLYSLNYMHWGAPKLWYGVGGKDAVKL 440

Query: 440 EVAM-KSLPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGF 499
           E AM K LP++F EQ   L   V+Q S + LK  G+P++RC+Q+ GEFVL FP   H+GF
Sbjct: 441 EEAMRKHLPDLFEEQPDLLHKLVTQLSPSKLKTAGVPVHRCVQHAGEFVLTFPRAYHAGF 500

Query: 500 NCGFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLC 559
           N GF+ +E  N AP+DWLP G  A+ELY  + RKT IS D+LLLGAA E VKA WEL+L 
Sbjct: 501 NSGFNCAEAVNVAPVDWLPHGQIAIELYCQQGRKTSISHDKLLLGAAREVVKADWELNLL 560

Query: 560 RKETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMREVTESFDAVGKRECS 619
           RK T DNLRWK    KDGILA+T K+RI  E  RRE+L  +S   ++  +FDA  +REC 
Sbjct: 561 RKNTVDNLRWKAFSAKDGILAKTLKARIDMERTRREFLCNSSLALKMHSNFDATNERECC 620

Query: 620 ICLYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLS 679
           IC +DLH SAAGC CS ++YSCL H K+LCSC W  ++FL R+ +  LN+L+EA+EGKLS
Sbjct: 621 ICFFDLHLSAAGCRCSPEKYSCLTHVKELCSCPWVTKYFLFRYDIDELNVLVEAVEGKLS 680

Query: 680 AVYKWAKENLGLAVHSYKNSSPQSLPAD-------SPQSS--KGQQSEDSESSNTVNSSI 739
           +VY+WA+++LGLA+ +  + S   +  +       +PQ++   G+  +   +S  V+  +
Sbjct: 681 SVYRWARQDLGLALSTDVSGSKMEIDEEGKVHKDPTPQTTALSGKDLQLKVTSKEVSKEL 740

Query: 740 NRIKAEIKARLLQSKTLKYKKENEKVIESEDAVKDNGTLTNSDSRIPA 756
            +        LL    LK K+E          VK+     +SD  + A
Sbjct: 741 EKTSKLSHVNLL----LKEKEEQITSSHCMKPVKEETVCDSSDPNVSA 782

BLAST of Lag0001991 vs. TAIR 10
Match: AT4G20400.1 (JUMONJI 14 )

HSP 1 Score: 486.5 bits (1251), Expect = 4.2e-137
Identity = 268/637 (42.07%), Postives = 371/637 (58.24%), Query Frame = 0

Query: 113 KVTARWHPEAARTDILEDAPVFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPP 172
           K+TARW+P  A   +++DAP+FYPT E+F D L YIE++R +AE  GICRIVPP +W PP
Sbjct: 38  KITARWNPSEACRPLVDDAPIFYPTNEDFDDPLGYIEKLRSKAESYGICRIVPPVAWRPP 97

Query: 173 CFLKEKEIWENSPFLAHYQRIDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPD 232
           C LKEK+IWENS F    Q ID  Q      +  +     + R  ++ Y    R     D
Sbjct: 98  CPLKEKKIWENSKFPTRIQFIDLLQNREPIKKSTKTKKRKRRRISKIGYTRRKR-----D 157

Query: 233 ESCGVDNKGPNS-------ELGREVTLKAFKSYADDFKSQYF-SCGNKVTNTEENSPLLQ 292
             C   + G +        + G + TL+ F+ Y + FK  YF S  +  +   EN     
Sbjct: 158 SGCDTASSGSSDSEGKFGFQTGPDFTLEEFQKYDEYFKECYFQSEDHPGSKASEN----- 217

Query: 293 EQWEPMVDQVEGEYRRIVENPTEQIEVLCGGSLGSHSLGSGFPPSSSPLIEPGHAD-HMD 352
           ++++P V  +EGEY RIVE  T+++EV  G  L +   GSGF P   P      AD +  
Sbjct: 218 KKFKPKVKDLEGEYWRIVEQATDEVEVYYGADLETKKFGSGF-PKYKPGYPISEADQYSQ 277

Query: 353 SGWNLNNLARLPGSLLSFDSFKTCSVLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAP 412
            GWNLNNL+RLPGS+L+F+S     V+ P +YVGMCFST CW VE+HHL  + YLH G P
Sbjct: 278 CGWNLNNLSRLPGSVLAFESCDISGVIVPWLYVGMCFSTFCWHVEDHHLYSMNYLHTGDP 337

Query: 413 KIWYGIPGRYFDKFEVAMKS-LPEIFLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPG 472
           K+WYGIPG + + FE  MK  LP++F EQ   L   V+Q S   LK EG+P+YR +Q  G
Sbjct: 338 KVWYGIPGNHAESFENVMKKRLPDLFEEQPDLLHQLVTQLSPRILKEEGVPVYRAVQRSG 397

Query: 473 EFVLVFPGVCHSGFNCGFSVSEEANFAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGA 532
           EF+L FP   HSGFNCGF+ +E  N AP+DWL  G NA+E YS +RRK+ +S D+LLLGA
Sbjct: 398 EFILTFPKAYHSGFNCGFNCAEAVNVAPVDWLVHGQNAVEGYSKQRRKSSLSHDKLLLGA 457

Query: 533 AVEAVKAQWELSLCRKETKDNLRWKNACGKDGILAQTFKSRIKSEGLRREYLTTASQMRE 592
           A+EA    WELSL +K+T    RWK  C +DG+L +  K R++ E  R  +L     +R+
Sbjct: 458 AMEATYCLWELSLSKKKTPVIARWKRVCSEDGLLTKAVKKRVQMEEERLNHLQDGFSLRK 517

Query: 593 VTESFDAVGKRECSICLYDLHFSAAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMG 652
           +   FD   +REC +C YDLH SA+ C CS +R++CL HAK LCSC    R+ L+RH + 
Sbjct: 518 MEGDFDNKRERECFLCFYDLHMSASSCKCSPNRFACLIHAKDLCSCESKDRYILIRHTLD 577

Query: 653 SLNLLLEALEGKLSAVYKWA--------KENLGLAVHSYKNSSPQSLPADSPQSSKGQQS 712
            L  L+ ALEG L A+  WA         ++     ++Y  S+P      S  SSK QQ 
Sbjct: 578 ELWALVRALEGDLDAIDLWASKCRDQYPSQHPRAREYAYLKSAP---CIKSRGSSKVQQR 637

Query: 713 EDSESSNTVNSSINRIKAEIKARLLQSKTLKYKKENE 732
           E         +++  +   +++ L  +K ++ K++ +
Sbjct: 638 E--------QNNLQLVSERLQSDLTSNKEVQLKQDGD 652

BLAST of Lag0001991 vs. TAIR 10
Match: AT1G30810.1 (Transcription factor jumonji (jmj) family protein / zinc finger (C5HC2 type) family protein )

HSP 1 Score: 483.0 bits (1242), Expect = 4.7e-136
Identity = 273/626 (43.61%), Postives = 356/626 (56.87%), Query Frame = 0

Query: 80  LEFEPMERDM-------NLPPQSDLPKGVAHGCPECSNCLKVTARWHPEAARTDILEDAP 139
           +E  P+E ++       N PP  D  K      P      KV ARW P+ A+  I+ DAP
Sbjct: 1   MENPPLESEIKEDMSLKNHPPDKDKDKDTIMEQPSSPRHRKVVARWLPDEAQRPIINDAP 60

Query: 140 VFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEKEIWENSPFLAHYQR 199
           VF P+ EEF D L YIE+IRP AE  GICRI+PP +W PPC LKEK IWE + F    Q 
Sbjct: 61  VFTPSLEEFVDPLAYIEKIRPLAEPYGICRIIPPSTWKPPCRLKEKSIWEQTKFPTRIQT 120

Query: 200 IDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESC----GVDNKGPNSELGR 259
           +D  Q      + P+     + R  R+      R    P ES       +  G NS  G 
Sbjct: 121 VDLLQNREPMKKKPKSRKRKRRRNSRMG-SSKRRSGSSPAESTSSPEAEEKFGFNS--GS 180

Query: 260 EVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEYRRIVENPTEQ 319
           + TL  F+ YA  FK  YF         +++S     +W P VD +EGEY RIVE PT++
Sbjct: 181 DFTLDEFEKYALHFKDSYFE--------KKDSGGDIVKWTPSVDDIEGEYWRIVEQPTDE 240

Query: 320 IEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSLLSFDSFKTCS 379
           +EV  G  L +  LGSGF   +          +  SGWNLNNL RLPGS+LSF+      
Sbjct: 241 VEVYYGADLENGVLGSGFYKRAEKFTGSDMEQYTLSGWNLNNLPRLPGSVLSFEDCDISG 300

Query: 380 VLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFEVAM-KSLPEI 439
           VL P +YVGMCFS+ CW VE+HHL  L Y H G PK+WYG+PG      E AM K LP++
Sbjct: 301 VLVPWLYVGMCFSSFCWHVEDHHLYSLNYHHFGEPKVWYGVPGSNATALEKAMRKHLPDL 360

Query: 440 FLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNCGFSVSEEAN 499
           F EQ   L   V+Q S + LK EG+  YR +QN GE+VL FP   H+GFNCGF+ +E  N
Sbjct: 361 FEEQPDLLHGLVTQFSPSILKDEGVQAYRVVQNSGEYVLTFPRAYHAGFNCGFNCAEAVN 420

Query: 500 FAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCR-KETKDNLRW 559
            AP+DWL  G NA+ELYS E RKT +S D+LLLGAA EAVKA WELS    KE   NLRW
Sbjct: 421 VAPVDWLAHGQNAVELYSKETRKTSLSHDKLLLGAAYEAVKALWELSASEGKENTTNLRW 480

Query: 560 KNACGKDGILAQTFKSRIKSEGLRREYL-TTASQMREVTESFDAVGKRECSICLYDLHFS 619
           K+ CGK+G L    ++R++ E  R   L   +S ++++ + FD+  +REC  C YDLH S
Sbjct: 481 KSFCGKNGTLTNAIQARLQMEEGRITALGRDSSSLKKMEKDFDSNCERECFSCFYDLHLS 540

Query: 620 AAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAVYKWAKEN 679
           A+GC CS + Y+CL HA  LCSC     F L+R+ M  L+ L+ ALEG+   +  WA + 
Sbjct: 541 ASGCKCSPEEYACLKHADDLCSCDVKDGFILLRYTMDELSSLVRALEGESDDLKIWASKV 600

Query: 680 LGLAVHSYKNSSPQSLPADSPQSSKG 692
           LG+       +   S+ ++  +  +G
Sbjct: 601 LGIEHSDEDQTKTSSVISEEKKLKEG 615

BLAST of Lag0001991 vs. TAIR 10
Match: AT1G30810.2 (Transcription factor jumonji (jmj) family protein / zinc finger (C5HC2 type) family protein )

HSP 1 Score: 483.0 bits (1242), Expect = 4.7e-136
Identity = 273/626 (43.61%), Postives = 356/626 (56.87%), Query Frame = 0

Query: 80  LEFEPMERDM-------NLPPQSDLPKGVAHGCPECSNCLKVTARWHPEAARTDILEDAP 139
           +E  P+E ++       N PP  D  K      P      KV ARW P+ A+  I+ DAP
Sbjct: 1   MENPPLESEIKEDMSLKNHPPDKDKDKDTIMEQPSSPRHRKVVARWLPDEAQRPIINDAP 60

Query: 140 VFYPTEEEFADTLHYIERIRPRAELCGICRIVPPPSWLPPCFLKEKEIWENSPFLAHYQR 199
           VF P+ EEF D L YIE+IRP AE  GICRI+PP +W PPC LKEK IWE + F    Q 
Sbjct: 61  VFTPSLEEFVDPLAYIEKIRPLAEPYGICRIIPPSTWKPPCRLKEKSIWEQTKFPTRIQT 120

Query: 200 IDGFQKNYACGQFPEHSGNIKNRWRRLEYECVNRCLMDPDESC----GVDNKGPNSELGR 259
           +D  Q      + P+     + R  R+      R    P ES       +  G NS  G 
Sbjct: 121 VDLLQNREPMKKKPKSRKRKRRRNSRMG-SSKRRSGSSPAESTSSPEAEEKFGFNS--GS 180

Query: 260 EVTLKAFKSYADDFKSQYFSCGNKVTNTEENSPLLQEQWEPMVDQVEGEYRRIVENPTEQ 319
           + TL  F+ YA  FK  YF         +++S     +W P VD +EGEY RIVE PT++
Sbjct: 181 DFTLDEFEKYALHFKDSYFE--------KKDSGGDIVKWTPSVDDIEGEYWRIVEQPTDE 240

Query: 320 IEVLCGGSLGSHSLGSGFPPSSSPLIEPGHADHMDSGWNLNNLARLPGSLLSFDSFKTCS 379
           +EV  G  L +  LGSGF   +          +  SGWNLNNL RLPGS+LSF+      
Sbjct: 241 VEVYYGADLENGVLGSGFYKRAEKFTGSDMEQYTLSGWNLNNLPRLPGSVLSFEDCDISG 300

Query: 380 VLSPRVYVGMCFSTACWRVEEHHLPLLCYLHLGAPKIWYGIPGRYFDKFEVAM-KSLPEI 439
           VL P +YVGMCFS+ CW VE+HHL  L Y H G PK+WYG+PG      E AM K LP++
Sbjct: 301 VLVPWLYVGMCFSSFCWHVEDHHLYSLNYHHFGEPKVWYGVPGSNATALEKAMRKHLPDL 360

Query: 440 FLEQKSFLIIQVSQPSIATLKREGIPIYRCIQNPGEFVLVFPGVCHSGFNCGFSVSEEAN 499
           F EQ   L   V+Q S + LK EG+  YR +QN GE+VL FP   H+GFNCGF+ +E  N
Sbjct: 361 FEEQPDLLHGLVTQFSPSILKDEGVQAYRVVQNSGEYVLTFPRAYHAGFNCGFNCAEAVN 420

Query: 500 FAPLDWLPFGYNAMELYSVERRKTLISFDRLLLGAAVEAVKAQWELSLCR-KETKDNLRW 559
            AP+DWL  G NA+ELYS E RKT +S D+LLLGAA EAVKA WELS    KE   NLRW
Sbjct: 421 VAPVDWLAHGQNAVELYSKETRKTSLSHDKLLLGAAYEAVKALWELSASEGKENTTNLRW 480

Query: 560 KNACGKDGILAQTFKSRIKSEGLRREYL-TTASQMREVTESFDAVGKRECSICLYDLHFS 619
           K+ CGK+G L    ++R++ E  R   L   +S ++++ + FD+  +REC  C YDLH S
Sbjct: 481 KSFCGKNGTLTNAIQARLQMEEGRITALGRDSSSLKKMEKDFDSNCERECFSCFYDLHLS 540

Query: 620 AAGCSCSTDRYSCLNHAKQLCSCAWGARFFLVRHKMGSLNLLLEALEGKLSAVYKWAKEN 679
           A+GC CS + Y+CL HA  LCSC     F L+R+ M  L+ L+ ALEG+   +  WA + 
Sbjct: 541 ASGCKCSPEEYACLKHADDLCSCDVKDGFILLRYTMDELSSLVRALEGESDDLKIWASKV 600

Query: 680 LGLAVHSYKNSSPQSLPADSPQSSKG 692
           LG+       +   S+ ++  +  +G
Sbjct: 601 LGIEHSDEDQTKTSSVISEEKKLKEG 615