Lag0001886 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0001886 vs. NCBI nr
Match: XP_038885260.1 (eIF-2-alpha kinase GCN2 isoform X4 [Benincasa hispida])

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 623/904 (68.92%), Postives = 643/904 (71.13%), Query Frame = 0

Query: 31  RHEVLIPAQDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMM-------- 90
           +HEVLIP QDSILT +VAE +DHG ESEPSEWSFASL+NEQESQT DRDIMM        
Sbjct: 159 QHEVLIPGQDSILTSNVAEGDDHGSESEPSEWSFASLNNEQESQTIDRDIMMVHLLHLAC 218

Query: 91  ---------------------VLSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN 150
                                VLSKVALDLASKPSS FHKKF+TAF++QMN+TSFSQFWN
Sbjct: 219 APKGPLADALPKLASELYNLGVLSKVALDLASKPSSTFHKKFKTAFQEQMNATSFSQFWN 278

Query: 151 PSDFGGPTSSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWL 210
           PSDFGG  SSQLSSRYLNDFEELK L                                  
Sbjct: 279 PSDFGGSASSQLSSRYLNDFEELKPL---------------------------------- 338

Query: 211 SLIFLRGLGIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKR 270
                                                                       
Sbjct: 339 ------------------------------------------------------------ 398

Query: 271 FASLGKWMWLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAF 330
                                                                       
Sbjct: 399 ------------------------------------------------------------ 458

Query: 331 YSWRLDSLKVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSG 390
                                                                      G
Sbjct: 459 -----------------------------------------------------------G 518

Query: 391 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 450
           HGGFGHVVLCKNKLDGR YAVKKIRLKDKILP+NDRILREVATLSRLQHQHVVRYYQAWY
Sbjct: 519 HGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPINDRILREVATLSRLQHQHVVRYYQAWY 578

Query: 451 ETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVF 510
           E+GVSDSYGEAAWGS+TPLSSTFSYKGASAT T EHENK+ESTYLYIQMEYCPRTLRQVF
Sbjct: 579 ESGVSDSYGEAAWGSVTPLSSTFSYKGASATDT-EHENKMESTYLYIQMEYCPRTLRQVF 638

Query: 511 ESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 570
           ESYTHFDKE AWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK
Sbjct: 639 ESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 698

Query: 571 LEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 630
           LEQLDQD+GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH
Sbjct: 699 LEQLDQDIGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 758

Query: 631 PFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPP 690
           PFGTAMERHLVLSDLKQKGELPT WVAEFSEQASLLRRLMSQSPS+RPSA ELLQ+AFPP
Sbjct: 759 PFGTAMERHLVLSDLKQKGELPTAWVAEFSEQASLLRRLMSQSPSDRPSALELLQNAFPP 818

Query: 691 RMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVR 750
           RMEYQLLDNILRTMK+SEDSSIYD+VVNAIFDEE+L MKDD+HDCGTA IQYTDLGTEVR
Sbjct: 819 RMEYQLLDNILRTMKSSEDSSIYDRVVNAIFDEETLVMKDDRHDCGTAPIQYTDLGTEVR 848

Query: 751 DHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLN 810
           DHVID TREMFRLHCAKHLEISSMYLLDGS QINRNTVKLLSHGGDILELCHELRLPFLN
Sbjct: 879 DHVIDATREMFRLHCAKHLEISSMYLLDGSTQINRNTVKLLSHGGDILELCHELRLPFLN 848

Query: 811 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 870
           WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY
Sbjct: 939 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 848

Query: 871 FFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 906
           FF+SDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR
Sbjct: 999 FFNSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 848

BLAST of Lag0001886 vs. NCBI nr
Match: XP_038885259.1 (eIF-2-alpha kinase GCN2 isoform X3 [Benincasa hispida])

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 623/904 (68.92%), Postives = 643/904 (71.13%), Query Frame = 0

Query: 31  RHEVLIPAQDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMM-------- 90
           +HEVLIP QDSILT +VAE +DHG ESEPSEWSFASL+NEQESQT DRDIMM        
Sbjct: 199 QHEVLIPGQDSILTSNVAEGDDHGSESEPSEWSFASLNNEQESQTIDRDIMMVHLLHLAC 258

Query: 91  ---------------------VLSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN 150
                                VLSKVALDLASKPSS FHKKF+TAF++QMN+TSFSQFWN
Sbjct: 259 APKGPLADALPKLASELYNLGVLSKVALDLASKPSSTFHKKFKTAFQEQMNATSFSQFWN 318

Query: 151 PSDFGGPTSSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWL 210
           PSDFGG  SSQLSSRYLNDFEELK L                                  
Sbjct: 319 PSDFGGSASSQLSSRYLNDFEELKPL---------------------------------- 378

Query: 211 SLIFLRGLGIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKR 270
                                                                       
Sbjct: 379 ------------------------------------------------------------ 438

Query: 271 FASLGKWMWLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAF 330
                                                                       
Sbjct: 439 ------------------------------------------------------------ 498

Query: 331 YSWRLDSLKVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSG 390
                                                                      G
Sbjct: 499 -----------------------------------------------------------G 558

Query: 391 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 450
           HGGFGHVVLCKNKLDGR YAVKKIRLKDKILP+NDRILREVATLSRLQHQHVVRYYQAWY
Sbjct: 559 HGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPINDRILREVATLSRLQHQHVVRYYQAWY 618

Query: 451 ETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVF 510
           E+GVSDSYGEAAWGS+TPLSSTFSYKGASAT T EHENK+ESTYLYIQMEYCPRTLRQVF
Sbjct: 619 ESGVSDSYGEAAWGSVTPLSSTFSYKGASATDT-EHENKMESTYLYIQMEYCPRTLRQVF 678

Query: 511 ESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 570
           ESYTHFDKE AWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK
Sbjct: 679 ESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 738

Query: 571 LEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 630
           LEQLDQD+GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH
Sbjct: 739 LEQLDQDIGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 798

Query: 631 PFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPP 690
           PFGTAMERHLVLSDLKQKGELPT WVAEFSEQASLLRRLMSQSPS+RPSA ELLQ+AFPP
Sbjct: 799 PFGTAMERHLVLSDLKQKGELPTAWVAEFSEQASLLRRLMSQSPSDRPSALELLQNAFPP 858

Query: 691 RMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVR 750
           RMEYQLLDNILRTMK+SEDSSIYD+VVNAIFDEE+L MKDD+HDCGTA IQYTDLGTEVR
Sbjct: 859 RMEYQLLDNILRTMKSSEDSSIYDRVVNAIFDEETLVMKDDRHDCGTAPIQYTDLGTEVR 888

Query: 751 DHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLN 810
           DHVID TREMFRLHCAKHLEISSMYLLDGS QINRNTVKLLSHGGDILELCHELRLPFLN
Sbjct: 919 DHVIDATREMFRLHCAKHLEISSMYLLDGSTQINRNTVKLLSHGGDILELCHELRLPFLN 888

Query: 811 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 870
           WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY
Sbjct: 979 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 888

Query: 871 FFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 906
           FF+SDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR
Sbjct: 1039 FFNSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 888

BLAST of Lag0001886 vs. NCBI nr
Match: XP_038885257.1 (eIF-2-alpha kinase GCN2 isoform X1 [Benincasa hispida])

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 623/904 (68.92%), Postives = 643/904 (71.13%), Query Frame = 0

Query: 31  RHEVLIPAQDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMM-------- 90
           +HEVLIP QDSILT +VAE +DHG ESEPSEWSFASL+NEQESQT DRDIMM        
Sbjct: 292 QHEVLIPGQDSILTSNVAEGDDHGSESEPSEWSFASLNNEQESQTIDRDIMMVHLLHLAC 351

Query: 91  ---------------------VLSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN 150
                                VLSKVALDLASKPSS FHKKF+TAF++QMN+TSFSQFWN
Sbjct: 352 APKGPLADALPKLASELYNLGVLSKVALDLASKPSSTFHKKFKTAFQEQMNATSFSQFWN 411

Query: 151 PSDFGGPTSSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWL 210
           PSDFGG  SSQLSSRYLNDFEELK L                                  
Sbjct: 412 PSDFGGSASSQLSSRYLNDFEELKPL---------------------------------- 471

Query: 211 SLIFLRGLGIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKR 270
                                                                       
Sbjct: 472 ------------------------------------------------------------ 531

Query: 271 FASLGKWMWLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAF 330
                                                                       
Sbjct: 532 ------------------------------------------------------------ 591

Query: 331 YSWRLDSLKVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSG 390
                                                                      G
Sbjct: 592 -----------------------------------------------------------G 651

Query: 391 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 450
           HGGFGHVVLCKNKLDGR YAVKKIRLKDKILP+NDRILREVATLSRLQHQHVVRYYQAWY
Sbjct: 652 HGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPINDRILREVATLSRLQHQHVVRYYQAWY 711

Query: 451 ETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVF 510
           E+GVSDSYGEAAWGS+TPLSSTFSYKGASAT T EHENK+ESTYLYIQMEYCPRTLRQVF
Sbjct: 712 ESGVSDSYGEAAWGSVTPLSSTFSYKGASATDT-EHENKMESTYLYIQMEYCPRTLRQVF 771

Query: 511 ESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 570
           ESYTHFDKE AWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK
Sbjct: 772 ESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 831

Query: 571 LEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 630
           LEQLDQD+GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH
Sbjct: 832 LEQLDQDIGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 891

Query: 631 PFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPP 690
           PFGTAMERHLVLSDLKQKGELPT WVAEFSEQASLLRRLMSQSPS+RPSA ELLQ+AFPP
Sbjct: 892 PFGTAMERHLVLSDLKQKGELPTAWVAEFSEQASLLRRLMSQSPSDRPSALELLQNAFPP 951

Query: 691 RMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVR 750
           RMEYQLLDNILRTMK+SEDSSIYD+VVNAIFDEE+L MKDD+HDCGTA IQYTDLGTEVR
Sbjct: 952 RMEYQLLDNILRTMKSSEDSSIYDRVVNAIFDEETLVMKDDRHDCGTAPIQYTDLGTEVR 981

Query: 751 DHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLN 810
           DHVID TREMFRLHCAKHLEISSMYLLDGS QINRNTVKLLSHGGDILELCHELRLPFLN
Sbjct: 1012 DHVIDATREMFRLHCAKHLEISSMYLLDGSTQINRNTVKLLSHGGDILELCHELRLPFLN 981

Query: 811 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 870
           WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY
Sbjct: 1072 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 981

Query: 871 FFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 906
           FF+SDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR
Sbjct: 1132 FFNSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 981

BLAST of Lag0001886 vs. NCBI nr
Match: XP_022150410.1 (eIF-2-alpha kinase GCN2 isoform X2 [Momordica charantia])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 613/904 (67.81%), Postives = 637/904 (70.46%), Query Frame = 0

Query: 31  RHEVLIPAQDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMM-------- 90
           +H+V +PAQDSILTDHVAED+DHG ESEPSEWSFASLSN+QESQTT+RDIMM        
Sbjct: 152 QHDVFMPAQDSILTDHVAEDDDHGSESEPSEWSFASLSNDQESQTTNRDIMMVHLLHLAC 211

Query: 91  ---------------------VLSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN 150
                                VLS+VALDL SKPSSMFHKKFETAF++QMN+TSFSQFWN
Sbjct: 212 APKGPLADALPKLASELYNLGVLSEVALDLTSKPSSMFHKKFETAFQEQMNATSFSQFWN 271

Query: 151 PSDFGGPTSSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWL 210
           PSDFGGPTSSQLSSRYLNDFEELK L                                  
Sbjct: 272 PSDFGGPTSSQLSSRYLNDFEELKPL---------------------------------- 331

Query: 211 SLIFLRGLGIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKR 270
                                                                       
Sbjct: 332 ------------------------------------------------------------ 391

Query: 271 FASLGKWMWLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAF 330
                                                                       
Sbjct: 392 ------------------------------------------------------------ 451

Query: 331 YSWRLDSLKVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSG 390
                                                                      G
Sbjct: 452 -----------------------------------------------------------G 511

Query: 391 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 450
           HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY
Sbjct: 512 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 571

Query: 451 ETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVF 510
           ETG SDSYGE AWGS TPLSSTFSYKG SA  TLEHE+K+ESTYLYIQMEYCPRTLRQ F
Sbjct: 572 ETGFSDSYGETAWGSTTPLSSTFSYKGVSAADTLEHESKLESTYLYIQMEYCPRTLRQDF 631

Query: 511 ESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 570
           ESYTHFDK+ AWHLFHQIVEGLAHIHGQGIIHRDLTP+NIFFDARNDIKIGDFGLAKFLK
Sbjct: 632 ESYTHFDKDLAWHLFHQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK 691

Query: 571 LEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 630
           LEQLDQD+GVP DT GVSIDGTGQVGTYFYTAPEIEQGWPKIDE ADMYSLGIVFFELWH
Sbjct: 692 LEQLDQDLGVPPDTIGVSIDGTGQVGTYFYTAPEIEQGWPKIDEMADMYSLGIVFFELWH 751

Query: 631 PFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPP 690
           PFGTAMERHLVLSDLKQKGE+P  W+AEF EQASLLRRLMSQSPSERPSA ELLQHAFPP
Sbjct: 752 PFGTAMERHLVLSDLKQKGEIPNAWIAEFPEQASLLRRLMSQSPSERPSALELLQHAFPP 811

Query: 691 RMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVR 750
           +MEYQLLDNILRTMKTSEDSSIYD+VVNAIFDEE+L  K DQHDCGTASIQYTDLGTEVR
Sbjct: 812 KMEYQLLDNILRTMKTSEDSSIYDRVVNAIFDEETLVTKGDQHDCGTASIQYTDLGTEVR 842

Query: 751 DHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLN 810
           DHVIDVTREMFRLHCAKHLEIS MYLLDGS QINRNTVK+L+HGGDILELCHELRLPFLN
Sbjct: 872 DHVIDVTREMFRLHCAKHLEISPMYLLDGSEQINRNTVKILTHGGDILELCHELRLPFLN 842

Query: 811 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 870
           W+VSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY
Sbjct: 932 WIVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 842

Query: 871 FFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 906
           FF+SDSCDIHLNHGDLLNAIWSW GVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR
Sbjct: 992 FFYSDSCDIHLNHGDLLNAIWSWAGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 842

BLAST of Lag0001886 vs. NCBI nr
Match: XP_022150409.1 (eIF-2-alpha kinase GCN2 isoform X1 [Momordica charantia])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 613/904 (67.81%), Postives = 637/904 (70.46%), Query Frame = 0

Query: 31  RHEVLIPAQDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMM-------- 90
           +H+V +PAQDSILTDHVAED+DHG ESEPSEWSFASLSN+QESQTT+RDIMM        
Sbjct: 282 QHDVFMPAQDSILTDHVAEDDDHGSESEPSEWSFASLSNDQESQTTNRDIMMVHLLHLAC 341

Query: 91  ---------------------VLSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN 150
                                VLS+VALDL SKPSSMFHKKFETAF++QMN+TSFSQFWN
Sbjct: 342 APKGPLADALPKLASELYNLGVLSEVALDLTSKPSSMFHKKFETAFQEQMNATSFSQFWN 401

Query: 151 PSDFGGPTSSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWL 210
           PSDFGGPTSSQLSSRYLNDFEELK L                                  
Sbjct: 402 PSDFGGPTSSQLSSRYLNDFEELKPL---------------------------------- 461

Query: 211 SLIFLRGLGIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKR 270
                                                                       
Sbjct: 462 ------------------------------------------------------------ 521

Query: 271 FASLGKWMWLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAF 330
                                                                       
Sbjct: 522 ------------------------------------------------------------ 581

Query: 331 YSWRLDSLKVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSG 390
                                                                      G
Sbjct: 582 -----------------------------------------------------------G 641

Query: 391 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 450
           HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY
Sbjct: 642 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 701

Query: 451 ETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVF 510
           ETG SDSYGE AWGS TPLSSTFSYKG SA  TLEHE+K+ESTYLYIQMEYCPRTLRQ F
Sbjct: 702 ETGFSDSYGETAWGSTTPLSSTFSYKGVSAADTLEHESKLESTYLYIQMEYCPRTLRQDF 761

Query: 511 ESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 570
           ESYTHFDK+ AWHLFHQIVEGLAHIHGQGIIHRDLTP+NIFFDARNDIKIGDFGLAKFLK
Sbjct: 762 ESYTHFDKDLAWHLFHQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK 821

Query: 571 LEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 630
           LEQLDQD+GVP DT GVSIDGTGQVGTYFYTAPEIEQGWPKIDE ADMYSLGIVFFELWH
Sbjct: 822 LEQLDQDLGVPPDTIGVSIDGTGQVGTYFYTAPEIEQGWPKIDEMADMYSLGIVFFELWH 881

Query: 631 PFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPP 690
           PFGTAMERHLVLSDLKQKGE+P  W+AEF EQASLLRRLMSQSPSERPSA ELLQHAFPP
Sbjct: 882 PFGTAMERHLVLSDLKQKGEIPNAWIAEFPEQASLLRRLMSQSPSERPSALELLQHAFPP 941

Query: 691 RMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVR 750
           +MEYQLLDNILRTMKTSEDSSIYD+VVNAIFDEE+L  K DQHDCGTASIQYTDLGTEVR
Sbjct: 942 KMEYQLLDNILRTMKTSEDSSIYDRVVNAIFDEETLVTKGDQHDCGTASIQYTDLGTEVR 972

Query: 751 DHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLN 810
           DHVIDVTREMFRLHCAKHLEIS MYLLDGS QINRNTVK+L+HGGDILELCHELRLPFLN
Sbjct: 1002 DHVIDVTREMFRLHCAKHLEISPMYLLDGSEQINRNTVKILTHGGDILELCHELRLPFLN 972

Query: 811 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 870
           W+VSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY
Sbjct: 1062 WIVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 972

Query: 871 FFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 906
           FF+SDSCDIHLNHGDLLNAIWSW GVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR
Sbjct: 1122 FFYSDSCDIHLNHGDLLNAIWSWAGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 972

BLAST of Lag0001886 vs. ExPASy Swiss-Prot
Match: Q9LX30 (eIF-2-alpha kinase GCN2 OS=Arabidopsis thaliana OX=3702 GN=GCN2 PE=2 SV=2)

HSP 1 Score: 848.6 bits (2191), Expect = 6.8e-245
Identity = 418/566 (73.85%), Postives = 472/566 (83.39%), Query Frame = 0

Query: 361 GHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAW 420
           G GGFGHVVLCKNKLDGRQYAVKKIRLKDK +PVN RI+REVATLSRLQHQHVVRYYQAW
Sbjct: 432 GQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVVRYYQAW 491

Query: 421 YETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQV 480
           +ETGV D +  A WGS T  SS FSY GA +T   E +N +ESTYLYIQMEYCPRTLRQV
Sbjct: 492 FETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPRTLRQV 551

Query: 481 FESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFL 540
           FESY HFDK+FAWHL  QIVEGLAHIHGQGIIHRD TP+NIFFDARNDIKIGDFGLAKFL
Sbjct: 552 FESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFGLAKFL 611

Query: 541 KLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELW 600
           KLEQLDQD G   D  G  +D TGQ GTYFYTAPEIEQ WPKIDEKADMYSLG+VFFELW
Sbjct: 612 KLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVVFFELW 671

Query: 601 HPFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFP 660
           HPFGTAMERH++L++LK KGELP  WV EF EQASLLRRLMS SPS+RPSA+ELL+HAFP
Sbjct: 672 HPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFP 731

Query: 661 PRMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHD----CGTAS-IQYTD 720
           PRME +LLDNILR M+TSEDSS+YD+VV+ IFDEE L MK  Q      C   S IQYT+
Sbjct: 732 PRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSHQSSRSRLCADDSYIQYTE 791

Query: 721 LGTEVRDHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHEL 780
           + TE+RD+V+++T+E+FR HCAKHLE+  M LL    Q +R TVKLL++GGD+LELC+EL
Sbjct: 792 INTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 851

Query: 781 RLPFLNWLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVT 840
           RLPF++W+  +QKSSFKRY+IS VYRRAIGHSPPN  LQ DFDI+GGT +LTEAEV+KV 
Sbjct: 852 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVI 911

Query: 841 VDVISYFFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSK 900
           VD+ ++ FH  SCDIHLNHGDLL+AIWSW G+KAEHR KVAELLSMM SLRPQSSERK K
Sbjct: 912 VDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLK 971

Query: 901 WVVIRRQLLQGESAVPHLDMHYLSVV 922
           WV IRRQLLQ E  +P   ++ L  V
Sbjct: 972 WVFIRRQLLQ-ELKLPEAVVNRLQTV 996

HSP 2 Score: 77.8 bits (190), Expect = 7.3e-13
Identity = 58/142 (40.85%), Postives = 72/142 (50.70%), Query Frame = 0

Query: 39  QDSILTDHVAEDEDHGYESEP-SEWSFASLSNEQESQTTDRDIMMV-------------- 98
           ++S L D  AED+    ESE    WS  SL+ +Q  Q + +D++MV              
Sbjct: 292 KESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLA 351

Query: 99  ---------------LSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN-PSDFGG 150
                          LS+  LDLASK S  F++ FE AF   M STS  QFW  PSD   
Sbjct: 352 DALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCE 411

BLAST of Lag0001886 vs. ExPASy Swiss-Prot
Match: Q9HGN1 (eIF-2-alpha kinase GCN2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gcn2 PE=2 SV=2)

HSP 1 Score: 247.3 bits (630), Expect = 6.9e-64
Identity = 210/672 (31.25%), Postives = 311/672 (46.28%), Query Frame = 0

Query: 337  KPSITGVIDGIFSLVGFK-------DHESGS--GHGGFGHVVLCKNKLDGRQYAVKKIRL 396
            +PS  G  DGI  L+  K       D E     G GGFG VV  KN++DGR YAVKK+ L
Sbjct: 530  RPSYGGQQDGIIDLLYRKSVSRYETDFEELEFLGRGGFGEVVKVKNRIDGRFYAVKKLVL 589

Query: 397  --KDKILPVNDRILREVATLSRLQHQHVVRYYQAWYETGVSDSYGEAAWGSMTPLSS--- 456
               DK    N RILREV TLSRL H+HVVRYY AW ET  +D+  E        LS    
Sbjct: 590  LSDDK---ENSRILREVMTLSRLHHEHVVRYYTAWVETEANDTVTEIISSDSESLSQSLN 649

Query: 457  -----------------------------------------TFSYKGAS----------- 516
                                                     +F Y   S           
Sbjct: 650  MAVDFRQSSSLPADKLSSLDIHFEDDYNSSADEEDPEASDISFQYSNTSDKEGSSDKDSS 709

Query: 517  ---ATGTLEHENKVESTYLYIQMEYCPR-TLRQVFESYTHFDKEFAWHLFHQIVEGLAHI 576
               A+     EN + +T LYIQMEYC + +L+ +       D+   W LF QI+E LA+I
Sbjct: 710  IEEASSVKTQENGLNAT-LYIQMEYCEKLSLQDIIRDKIPVDE--MWRLFRQILEALAYI 769

Query: 577  HGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDVGVPLDTTGVSIDGTGQV 636
            H +G++HRDL P NIF D   ++K+GDFGLA   +  Q + D     +      D T  V
Sbjct: 770  HSRGMMHRDLKPGNIFLDENRNVKLGDFGLATENENYQDNNDKW--KNRQSADEDLTTGV 829

Query: 637  GTYFYTAPEI--EQGWPKIDEKADMYSLGIVFFELWHPFGTAMERHLVLSDLKQKG-ELP 696
            GT  Y APE+   +   + D K DMYSLGI+ FE+   F T+MER  ++  ++      P
Sbjct: 830  GTALYVAPELLSRRNGVRYDAKVDMYSLGIILFEMCMTFSTSMERIRIIDTIRSPSISFP 889

Query: 697  TTW-VAEFSEQASLLRRLMSQSPSERPSASELLQ-HAFPPRMEYQLLDNILRTMKTSEDS 756
            +T+  +  S +  ++  L+   P++RPS+ ELL+  A PP++  + +   LR + ++ ++
Sbjct: 890  STFPFSRASHEFKVIHCLLQHDPTKRPSSQELLESEAIPPKVGEEFIQEGLRLL-SNPNT 949

Query: 757  SIYDKVVNAIFDEESLTMKDDQHDCGTAS-----IQYTDLGTE------VRDHVIDVTRE 816
              Y K++  +F +     KD  +D   +       + +D G +      VRDHV+ V   
Sbjct: 950  PYYLKLLKVLFGQVPDRHKDFTYDFNLSEESGVLSKVSDRGWDSLLACLVRDHVVKV--- 1009

Query: 817  MFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLNWLVSSQKSS 876
             FR H AK  E   ++        ++ +V LL   G +L+L ++  LP+   +  +    
Sbjct: 1010 -FRRHGAKERESHILFPKSSQYDKDQASVSLLDKNGTLLQLPYDTVLPYARNVARNAVEE 1069

Query: 877  FKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALT--EAEVIKVTVDVISYFFHSDSC 921
             K Y IS V+R A G   P    +  FDI   +  L   +AE IK   +V++        
Sbjct: 1070 EKTYLISDVFREAKGGGRPKAIKEISFDITTNSDNLDWYDAETIKALDEVLTEIPSLTES 1129

BLAST of Lag0001886 vs. ExPASy Swiss-Prot
Match: Q9P2K8 (eIF-2-alpha kinase GCN2 OS=Homo sapiens OX=9606 GN=EIF2AK4 PE=1 SV=3)

HSP 1 Score: 216.9 bits (551), Expect = 1.0e-54
Identity = 194/653 (29.71%), Postives = 294/653 (45.02%), Query Frame = 0

Query: 361  GHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAW 420
            G G FG V+  +NKLDG  YAVK+I +         RI  EV  LSRL H+++VRYY AW
Sbjct: 597  GKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFR-RIKGEVTLLSRLHHENIVRYYNAW 656

Query: 421  YE--------------------------------TGVSDSYGEAAWGSMTPLSSTFSYKG 480
             E                                T   DS   AA   +   S  +S  G
Sbjct: 657  IERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSG 716

Query: 481  ---------ASATGTLE------------------------------------------- 540
                     A+  G+ +                                           
Sbjct: 717  ERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDE 776

Query: 541  --------HENKVEST-----YLYIQMEYCPR-TLRQVFESYTHFDKEFAWHLFHQIVEG 600
                    HE++   T     YLYIQMEYC + TLR   +   + D    W LF +I++G
Sbjct: 777  DCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDG 836

Query: 601  LAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLA----KFLKLEQLDQDVGVPLDTTGV 660
            LA+IH +G+IHRDL P NIF D+ + +KIGDFGLA     F    + D   G  L  +  
Sbjct: 837  LAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTG-DLIKSDP 896

Query: 661  SIDGTGQVGTYFYTAPEIEQGWPK--IDEKADMYSLGIVFFEL-WHPFGTAMERHLVLSD 720
            S   TG VGT  Y +PE+ QG  K   ++K D++SLGI+FFE+ +HP  TA ER  VL+ 
Sbjct: 897  SGHLTGMVGTALYVSPEV-QGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQ 956

Query: 721  LKQ--KGELPTTW-VAEFSEQASLLRRLMSQSPSERPSASELLQHAF--PPRMEYQLLDN 780
            L+     + P  +   E ++Q S++  L++  P++RP+A+ELL+     PP+ME   L  
Sbjct: 957  LRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHE 1016

Query: 781  ILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVRDHVIDVTRE 840
            +L    T+ D   Y  ++  IF +      D  +D       ++    +++ HV +    
Sbjct: 1017 VLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIR 1076

Query: 841  MFRLHCAKHLEISSMYLLDGSMQI--NRNTVKLLSHGGDILELCHELRLPFLNWLVSSQK 897
            +F+ H A  +++ +  LL  + QI  +      + H G ++ L  +LR+PF  ++  +  
Sbjct: 1077 IFKRHGA--VQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNI 1136

BLAST of Lag0001886 vs. ExPASy Swiss-Prot
Match: Q75JN1 (Probable serine/threonine-protein kinase ifkC OS=Dictyostelium discoideum OX=44689 GN=ifkC PE=3 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 2.9e-54
Identity = 215/888 (24.21%), Postives = 332/888 (37.39%), Query Frame = 0

Query: 361  GHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAW 420
            G GGFG VV  +NKLDGR YA+KKI+L D    +N RILREV TLSRL HQHVVRYYQAW
Sbjct: 501  GRGGFGQVVKVRNKLDGRYYAIKKIKL-DSNQSLNRRILREVITLSRLHHQHVVRYYQAW 560

Query: 421  YETGVS-------------------------DSYGEAAWGSMTPLSSTFSYKGASATGTL 480
             E+  S                         +S  E+   S     S+ S + + ++ + 
Sbjct: 561  IESAESLSLTNDNSDDDDDDDDEFESGSESEESQSESESESSESEESSESEESSESSESE 620

Query: 481  EHE--------------------------------------------------------- 540
            E E                                                         
Sbjct: 621  ESEESEYSDDSEFESEENNDFDQSFSEVFLFQNGNKRSLEFDLTDDSYSFLHSDCGFLLE 680

Query: 541  -----NKV---------------------------------------------------- 600
                 NK+                                                    
Sbjct: 681  VFDLNNKIGGNNTRSMGSSNQHLQQQQQQNQSQQQKKQPQQNQSQQQKKLKNSNSKSKSK 740

Query: 601  --------------------------EST---YLYIQMEYCPRTLRQVFESYTHFDKEFA 660
                                      +ST   YLYIQMEYC + LR + E+  + + +  
Sbjct: 741  SKSKSKSKSKSNSKKSISSSKLKNIKKSTSIPYLYIQMEYCQKILRNLTETGMNLEDDDI 800

Query: 661  WHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK-LEQLDQDVGV 720
            W LF QIVEG+A++HGQGIIHRDL PSNIFFD+  DIKIGDFGLA   K    L     +
Sbjct: 801  WKLFRQIVEGMAYVHGQGIIHRDLKPSNIFFDSCGDIKIGDFGLAINNKTTSTLSPTTNI 860

Query: 721  PLDTTGV-------------------------------SIDG------------------ 780
               T+                                  ++G                  
Sbjct: 861  NSSTSSAGSLTTSSNSVQSVINTKQQQQQQQPQNYWEDEVEGQQQQQQQQQQQQQQQQQQ 920

Query: 781  -------TGQVGTYFYTAPEIEQGW---PKIDEKADMYSLGIVFFELWHPFGTAMERHLV 840
                   T +VGT FYT+PE E G       D+K DMYSLGIVFFE+W+ F T  ER +V
Sbjct: 921  QQQQQQHTARVGTLFYTSPEQEAGTNGDSAYDDKVDMYSLGIVFFEMWYVFSTGHERVIV 980

Query: 841  LSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQ-HAFPPRMEYQLLDNI 900
            L +L++K E P+ +    S QA+L+R L+ + P++RPSA +LLQ    PP+ME + + N 
Sbjct: 981  LRNLREKFEFPSDFERNHSRQATLIRMLIDKDPAKRPSAQQLLQSELMPPKMEDEYIKNS 1040

Query: 901  LRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLG---------TEVRD 906
            +R + T+  +  Y  ++N++F +    +    +    +S+  +             +V+ 
Sbjct: 1041 IRVI-TNPTNQFYQTMLNSLFSQNHQHLHSHIYHHQQSSVTSSSYSKSLFCSLDLLQVKQ 1100

BLAST of Lag0001886 vs. ExPASy Swiss-Prot
Match: Q9QZ05 (eIF-2-alpha kinase GCN2 OS=Mus musculus OX=10090 GN=Eif2ak4 PE=1 SV=2)

HSP 1 Score: 213.8 bits (543), Expect = 8.5e-54
Identity = 188/658 (28.57%), Postives = 291/658 (44.22%), Query Frame = 0

Query: 361  GHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVND------RILREVATLSRLQHQHVV 420
            G G FG V+  +NKLDG  YAVK+I       P+N       RI  EV  LSRL H+++V
Sbjct: 596  GKGAFGAVIKVQNKLDGCCYAVKRI-------PINPASRHFRRIKGEVTLLSRLHHENIV 655

Query: 421  RYYQAWYE-----------------------------TGVSDSYGEAAWGSMTPL----- 480
            RYY AW E                             +G ++  G     +  P+     
Sbjct: 656  RYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSV 715

Query: 481  ----------SSTFSYKGASATGTLEHENKVEST-------------------------- 540
                      S+ F   G  ++   E E++ +                            
Sbjct: 716  EWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKS 775

Query: 541  --------------------------YLYIQMEYCPR-TLRQVFESYTHFDKEFAWHLFH 600
                                      YLYIQMEYC + TLR   +     D    W LF 
Sbjct: 776  QNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFR 835

Query: 601  QIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLA----KFLKLEQLDQDVGVPL 660
            +I++GLA+IH +G+IHRDL P NIF D+ + +KIGDFGLA     F    + D   G  +
Sbjct: 836  EILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGV 895

Query: 661  DTTGVSIDGTGQVGTYFYTAPEIEQGWPK--IDEKADMYSLGIVFFEL-WHPFGTAMERH 720
              +  S   TG VGT  Y +PE+ QG  K   ++K D++SLGI+FFE+ +HP  TA ER 
Sbjct: 896  IKSDPSGHLTGMVGTALYVSPEV-QGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERI 955

Query: 721  LVLSDLKQ--KGELPTTW-VAEFSEQASLLRRLMSQSPSERPSASELLQHAF--PPRMEY 780
             VL+ L+     + P  +   E ++Q S++  L++  P++RP+A ELL+     PP+ME 
Sbjct: 956  FVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEE 1015

Query: 781  QLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVRDHVI 840
              L  +L     + D   Y  +++ IF +      D  +D       +     +++  V 
Sbjct: 1016 SELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVC 1075

Query: 841  DVTREMFRLHCAKHLEISSMYLLDGSMQI--NRNTVKLLSHGGDILELCHELRLPFLNWL 897
            +    +F+ H A  +++ +  LL  + QI  +      + H G ++ L  +LR+PF  ++
Sbjct: 1076 ETIVRVFKRHGA--VQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYV 1135

BLAST of Lag0001886 vs. ExPASy TrEMBL
Match: A0A6J1D9Z7 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111018572 PE=4 SV=1)

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 613/904 (67.81%), Postives = 637/904 (70.46%), Query Frame = 0

Query: 31  RHEVLIPAQDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMM-------- 90
           +H+V +PAQDSILTDHVAED+DHG ESEPSEWSFASLSN+QESQTT+RDIMM        
Sbjct: 282 QHDVFMPAQDSILTDHVAEDDDHGSESEPSEWSFASLSNDQESQTTNRDIMMVHLLHLAC 341

Query: 91  ---------------------VLSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN 150
                                VLS+VALDL SKPSSMFHKKFETAF++QMN+TSFSQFWN
Sbjct: 342 APKGPLADALPKLASELYNLGVLSEVALDLTSKPSSMFHKKFETAFQEQMNATSFSQFWN 401

Query: 151 PSDFGGPTSSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWL 210
           PSDFGGPTSSQLSSRYLNDFEELK L                                  
Sbjct: 402 PSDFGGPTSSQLSSRYLNDFEELKPL---------------------------------- 461

Query: 211 SLIFLRGLGIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKR 270
                                                                       
Sbjct: 462 ------------------------------------------------------------ 521

Query: 271 FASLGKWMWLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAF 330
                                                                       
Sbjct: 522 ------------------------------------------------------------ 581

Query: 331 YSWRLDSLKVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSG 390
                                                                      G
Sbjct: 582 -----------------------------------------------------------G 641

Query: 391 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 450
           HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY
Sbjct: 642 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 701

Query: 451 ETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVF 510
           ETG SDSYGE AWGS TPLSSTFSYKG SA  TLEHE+K+ESTYLYIQMEYCPRTLRQ F
Sbjct: 702 ETGFSDSYGETAWGSTTPLSSTFSYKGVSAADTLEHESKLESTYLYIQMEYCPRTLRQDF 761

Query: 511 ESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 570
           ESYTHFDK+ AWHLFHQIVEGLAHIHGQGIIHRDLTP+NIFFDARNDIKIGDFGLAKFLK
Sbjct: 762 ESYTHFDKDLAWHLFHQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK 821

Query: 571 LEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 630
           LEQLDQD+GVP DT GVSIDGTGQVGTYFYTAPEIEQGWPKIDE ADMYSLGIVFFELWH
Sbjct: 822 LEQLDQDLGVPPDTIGVSIDGTGQVGTYFYTAPEIEQGWPKIDEMADMYSLGIVFFELWH 881

Query: 631 PFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPP 690
           PFGTAMERHLVLSDLKQKGE+P  W+AEF EQASLLRRLMSQSPSERPSA ELLQHAFPP
Sbjct: 882 PFGTAMERHLVLSDLKQKGEIPNAWIAEFPEQASLLRRLMSQSPSERPSALELLQHAFPP 941

Query: 691 RMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVR 750
           +MEYQLLDNILRTMKTSEDSSIYD+VVNAIFDEE+L  K DQHDCGTASIQYTDLGTEVR
Sbjct: 942 KMEYQLLDNILRTMKTSEDSSIYDRVVNAIFDEETLVTKGDQHDCGTASIQYTDLGTEVR 972

Query: 751 DHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLN 810
           DHVIDVTREMFRLHCAKHLEIS MYLLDGS QINRNTVK+L+HGGDILELCHELRLPFLN
Sbjct: 1002 DHVIDVTREMFRLHCAKHLEISPMYLLDGSEQINRNTVKILTHGGDILELCHELRLPFLN 972

Query: 811 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 870
           W+VSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY
Sbjct: 1062 WIVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 972

Query: 871 FFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 906
           FF+SDSCDIHLNHGDLLNAIWSW GVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR
Sbjct: 1122 FFYSDSCDIHLNHGDLLNAIWSWAGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 972

BLAST of Lag0001886 vs. ExPASy TrEMBL
Match: A0A6J1D8E1 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111018572 PE=4 SV=1)

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 613/904 (67.81%), Postives = 637/904 (70.46%), Query Frame = 0

Query: 31  RHEVLIPAQDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMM-------- 90
           +H+V +PAQDSILTDHVAED+DHG ESEPSEWSFASLSN+QESQTT+RDIMM        
Sbjct: 152 QHDVFMPAQDSILTDHVAEDDDHGSESEPSEWSFASLSNDQESQTTNRDIMMVHLLHLAC 211

Query: 91  ---------------------VLSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN 150
                                VLS+VALDL SKPSSMFHKKFETAF++QMN+TSFSQFWN
Sbjct: 212 APKGPLADALPKLASELYNLGVLSEVALDLTSKPSSMFHKKFETAFQEQMNATSFSQFWN 271

Query: 151 PSDFGGPTSSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWL 210
           PSDFGGPTSSQLSSRYLNDFEELK L                                  
Sbjct: 272 PSDFGGPTSSQLSSRYLNDFEELKPL---------------------------------- 331

Query: 211 SLIFLRGLGIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKR 270
                                                                       
Sbjct: 332 ------------------------------------------------------------ 391

Query: 271 FASLGKWMWLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAF 330
                                                                       
Sbjct: 392 ------------------------------------------------------------ 451

Query: 331 YSWRLDSLKVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSG 390
                                                                      G
Sbjct: 452 -----------------------------------------------------------G 511

Query: 391 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 450
           HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY
Sbjct: 512 HGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWY 571

Query: 451 ETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVF 510
           ETG SDSYGE AWGS TPLSSTFSYKG SA  TLEHE+K+ESTYLYIQMEYCPRTLRQ F
Sbjct: 572 ETGFSDSYGETAWGSTTPLSSTFSYKGVSAADTLEHESKLESTYLYIQMEYCPRTLRQDF 631

Query: 511 ESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK 570
           ESYTHFDK+ AWHLFHQIVEGLAHIHGQGIIHRDLTP+NIFFDARNDIKIGDFGLAKFLK
Sbjct: 632 ESYTHFDKDLAWHLFHQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK 691

Query: 571 LEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 630
           LEQLDQD+GVP DT GVSIDGTGQVGTYFYTAPEIEQGWPKIDE ADMYSLGIVFFELWH
Sbjct: 692 LEQLDQDLGVPPDTIGVSIDGTGQVGTYFYTAPEIEQGWPKIDEMADMYSLGIVFFELWH 751

Query: 631 PFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPP 690
           PFGTAMERHLVLSDLKQKGE+P  W+AEF EQASLLRRLMSQSPSERPSA ELLQHAFPP
Sbjct: 752 PFGTAMERHLVLSDLKQKGEIPNAWIAEFPEQASLLRRLMSQSPSERPSALELLQHAFPP 811

Query: 691 RMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVR 750
           +MEYQLLDNILRTMKTSEDSSIYD+VVNAIFDEE+L  K DQHDCGTASIQYTDLGTEVR
Sbjct: 812 KMEYQLLDNILRTMKTSEDSSIYDRVVNAIFDEETLVTKGDQHDCGTASIQYTDLGTEVR 842

Query: 751 DHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLN 810
           DHVIDVTREMFRLHCAKHLEIS MYLLDGS QINRNTVK+L+HGGDILELCHELRLPFLN
Sbjct: 872 DHVIDVTREMFRLHCAKHLEISPMYLLDGSEQINRNTVKILTHGGDILELCHELRLPFLN 842

Query: 811 WLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 870
           W+VSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY
Sbjct: 932 WIVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISY 842

Query: 871 FFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 906
           FF+SDSCDIHLNHGDLLNAIWSW GVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR
Sbjct: 992 FFYSDSCDIHLNHGDLLNAIWSWAGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRR 842

BLAST of Lag0001886 vs. ExPASy TrEMBL
Match: A0A6J1H7A8 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111460823 PE=4 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 616/896 (68.75%), Postives = 635/896 (70.87%), Query Frame = 0

Query: 39  QDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMMV--------------- 98
           QDSI+ DH AED+DHG ESEPSEWSFAS+S EQESQTTDRDIMMV               
Sbjct: 157 QDSIIVDHAAEDDDHGSESEPSEWSFASMSKEQESQTTDRDIMMVHLLHLACAPKGPLAD 216

Query: 99  --------------LSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWNPSDFGGPT 158
                         LSKVALDLASKPSSMFHKKFE+AF+++MN+TSFS+FWNPSDFGGPT
Sbjct: 217 ALPKLASELYNSGILSKVALDLASKPSSMFHKKFESAFQEEMNATSFSEFWNPSDFGGPT 276

Query: 159 SSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWLSLIFLRGL 218
           SSQLSSRYLNDFEELK L                                          
Sbjct: 277 SSQLSSRYLNDFEELKPL------------------------------------------ 336

Query: 219 GIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKRFASLGKWM 278
                                                                       
Sbjct: 337 ------------------------------------------------------------ 396

Query: 279 WLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAFYSWRLDSL 338
                                                                       
Sbjct: 397 ------------------------------------------------------------ 456

Query: 339 KVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSGHGGFGHVV 398
                                                              GHGGFGHVV
Sbjct: 457 ---------------------------------------------------GHGGFGHVV 516

Query: 399 LCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWYETGVSDSY 458
           LCKNKLDGRQYAVKKIRLKDKILPV+DRILREVATLSRLQHQHVVRYYQAW E GVSDSY
Sbjct: 517 LCKNKLDGRQYAVKKIRLKDKILPVDDRILREVATLSRLQHQHVVRYYQAWSEAGVSDSY 576

Query: 459 GEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVFESYTHFDK 518
           GEAAWGS TPLSSTFSYKGASAT TL+HENK+ESTYLYIQMEYCPRTLRQ FESYTHFDK
Sbjct: 577 GEAAWGSTTPLSSTFSYKGASATDTLDHENKLESTYLYIQMEYCPRTLRQDFESYTHFDK 636

Query: 519 EFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDV 578
           E AWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDV
Sbjct: 637 ELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDV 696

Query: 579 GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER 638
           GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER
Sbjct: 697 GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER 756

Query: 639 HLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPPRMEYQLLD 698
           HLVLSDLKQKGELPTTWVAEF EQASLLRRLMSQSPSERPSA ELLQHAFPPRMEYQLLD
Sbjct: 757 HLVLSDLKQKGELPTTWVAEFPEQASLLRRLMSQSPSERPSALELLQHAFPPRMEYQLLD 816

Query: 699 NILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVRDHVIDVTR 758
           NILRTMKTSEDSSIYD+VVNAIF EE+L +KDDQ DCGTASIQYTDLGTEVRDHVIDVTR
Sbjct: 817 NILRTMKTSEDSSIYDRVVNAIFSEETLAIKDDQLDCGTASIQYTDLGTEVRDHVIDVTR 839

Query: 759 EMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLNWLVSSQKS 818
           E+FRLHCAKHLEIS M+LLDGS QINRNTVKLLSHGGDILELCHELRLPFLNWLVSSQKS
Sbjct: 877 ELFRLHCAKHLEISPMHLLDGSAQINRNTVKLLSHGGDILELCHELRLPFLNWLVSSQKS 839

Query: 819 SFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHSDSCD 878
           SFKRYDI+CVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHS+SCD
Sbjct: 937 SFKRYDIACVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHSESCD 839

Query: 879 IHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQ 906
           IHLNHGDLLNAIWSWVGVKAE RHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQ
Sbjct: 997 IHLNHGDLLNAIWSWVGVKAEQRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQ 839

BLAST of Lag0001886 vs. ExPASy TrEMBL
Match: A0A6J1H5V7 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111460823 PE=4 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 616/896 (68.75%), Postives = 635/896 (70.87%), Query Frame = 0

Query: 39  QDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMMV--------------- 98
           QDSI+ DH AED+DHG ESEPSEWSFAS+S EQESQTTDRDIMMV               
Sbjct: 290 QDSIIVDHAAEDDDHGSESEPSEWSFASMSKEQESQTTDRDIMMVHLLHLACAPKGPLAD 349

Query: 99  --------------LSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWNPSDFGGPT 158
                         LSKVALDLASKPSSMFHKKFE+AF+++MN+TSFS+FWNPSDFGGPT
Sbjct: 350 ALPKLASELYNSGILSKVALDLASKPSSMFHKKFESAFQEEMNATSFSEFWNPSDFGGPT 409

Query: 159 SSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWLSLIFLRGL 218
           SSQLSSRYLNDFEELK L                                          
Sbjct: 410 SSQLSSRYLNDFEELKPL------------------------------------------ 469

Query: 219 GIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKRFASLGKWM 278
                                                                       
Sbjct: 470 ------------------------------------------------------------ 529

Query: 279 WLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAFYSWRLDSL 338
                                                                       
Sbjct: 530 ------------------------------------------------------------ 589

Query: 339 KVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSGHGGFGHVV 398
                                                              GHGGFGHVV
Sbjct: 590 ---------------------------------------------------GHGGFGHVV 649

Query: 399 LCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWYETGVSDSY 458
           LCKNKLDGRQYAVKKIRLKDKILPV+DRILREVATLSRLQHQHVVRYYQAW E GVSDSY
Sbjct: 650 LCKNKLDGRQYAVKKIRLKDKILPVDDRILREVATLSRLQHQHVVRYYQAWSEAGVSDSY 709

Query: 459 GEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVFESYTHFDK 518
           GEAAWGS TPLSSTFSYKGASAT TL+HENK+ESTYLYIQMEYCPRTLRQ FESYTHFDK
Sbjct: 710 GEAAWGSTTPLSSTFSYKGASATDTLDHENKLESTYLYIQMEYCPRTLRQDFESYTHFDK 769

Query: 519 EFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDV 578
           E AWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDV
Sbjct: 770 ELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDV 829

Query: 579 GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER 638
           GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER
Sbjct: 830 GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER 889

Query: 639 HLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPPRMEYQLLD 698
           HLVLSDLKQKGELPTTWVAEF EQASLLRRLMSQSPSERPSA ELLQHAFPPRMEYQLLD
Sbjct: 890 HLVLSDLKQKGELPTTWVAEFPEQASLLRRLMSQSPSERPSALELLQHAFPPRMEYQLLD 949

Query: 699 NILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVRDHVIDVTR 758
           NILRTMKTSEDSSIYD+VVNAIF EE+L +KDDQ DCGTASIQYTDLGTEVRDHVIDVTR
Sbjct: 950 NILRTMKTSEDSSIYDRVVNAIFSEETLAIKDDQLDCGTASIQYTDLGTEVRDHVIDVTR 972

Query: 759 EMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLNWLVSSQKS 818
           E+FRLHCAKHLEIS M+LLDGS QINRNTVKLLSHGGDILELCHELRLPFLNWLVSSQKS
Sbjct: 1010 ELFRLHCAKHLEISPMHLLDGSAQINRNTVKLLSHGGDILELCHELRLPFLNWLVSSQKS 972

Query: 819 SFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHSDSCD 878
           SFKRYDI+CVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHS+SCD
Sbjct: 1070 SFKRYDIACVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHSESCD 972

Query: 879 IHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQ 906
           IHLNHGDLLNAIWSWVGVKAE RHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQ
Sbjct: 1130 IHLNHGDLLNAIWSWVGVKAEQRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQ 972

BLAST of Lag0001886 vs. ExPASy TrEMBL
Match: A0A6J1KZ64 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111497683 PE=4 SV=1)

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 614/896 (68.53%), Postives = 633/896 (70.65%), Query Frame = 0

Query: 39  QDSILTDHVAEDEDHGYESEPSEWSFASLSNEQESQTTDRDIMMV--------------- 98
           QDSI+ DH AED+DHG ESEPSEWSFAS+S EQESQTTDRDIMMV               
Sbjct: 290 QDSIIVDHAAEDDDHGSESEPSEWSFASMSKEQESQTTDRDIMMVHLLHLACAPKGPLAD 349

Query: 99  --------------LSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWNPSDFGGPT 158
                         LSKVALDLASKPSS FHKKFE+AF+++MN+TSFS+FWNPSDFGGPT
Sbjct: 350 ALPKLASELYNSGILSKVALDLASKPSSTFHKKFESAFQEEMNATSFSEFWNPSDFGGPT 409

Query: 159 SSQLSSRYLNDFEELKSLGKHRSLAFWDSMIDKFRSKIAKWKNSILSKGGWLSLIFLRGL 218
           SSQLSSRYLNDFEELK L                                          
Sbjct: 410 SSQLSSRYLNDFEELKPL------------------------------------------ 469

Query: 219 GIGSLEHRNSALLTKCSQNCLWFDSVKLMSQYEKFIRFKDATGDLELEYKKRFASLGKWM 278
                                                                       
Sbjct: 470 ------------------------------------------------------------ 529

Query: 279 WLVESLEAWKMKGKSKVCWKFASRTLFWSLWLEWNQSVGLSTCLPNILMIAFYSWRLDSL 338
                                                                       
Sbjct: 530 ------------------------------------------------------------ 589

Query: 339 KVVHGWKMKGKVKILWGCVVRAFLWLLKPSITGVIDGIFSLVGFKDHESGSGHGGFGHVV 398
                                                              GHGGFGHVV
Sbjct: 590 ---------------------------------------------------GHGGFGHVV 649

Query: 399 LCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWYETGVSDSY 458
           LCKNKLDGRQYAVKKIRLKDKILPV+DRILREV+TLSRLQHQHVVRYYQAW E GVSDSY
Sbjct: 650 LCKNKLDGRQYAVKKIRLKDKILPVDDRILREVSTLSRLQHQHVVRYYQAWSEAGVSDSY 709

Query: 459 GEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQVFESYTHFDK 518
           GEAAWGS TPLSSTFSYKGASAT  L+HENK+ESTYLYIQMEYCPRTLRQ FESYTHFDK
Sbjct: 710 GEAAWGSTTPLSSTFSYKGASATDALDHENKLESTYLYIQMEYCPRTLRQDFESYTHFDK 769

Query: 519 EFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDV 578
           E AWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDV
Sbjct: 770 ELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDV 829

Query: 579 GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER 638
           GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER
Sbjct: 830 GVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER 889

Query: 639 HLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFPPRMEYQLLD 698
           HLVLSDLKQKGELPTTWVAEF EQASLLRRLMSQSPSERPSA ELLQHAFPPRMEYQLLD
Sbjct: 890 HLVLSDLKQKGELPTTWVAEFPEQASLLRRLMSQSPSERPSALELLQHAFPPRMEYQLLD 949

Query: 699 NILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHDCGTASIQYTDLGTEVRDHVIDVTR 758
           NILRTMKTSEDSSIYD+VVNAIF EE+L MKDDQ DCGTASIQYTDLGTEVRDHVIDVTR
Sbjct: 950 NILRTMKTSEDSSIYDRVVNAIFSEETLVMKDDQLDCGTASIQYTDLGTEVRDHVIDVTR 972

Query: 759 EMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHELRLPFLNWLVSSQKS 818
           E+FRLHCAKHLEIS M+LLDGS QINRNTVKLLSHGGDILELCHELRLPFLNWLVS QKS
Sbjct: 1010 ELFRLHCAKHLEISPMHLLDGSAQINRNTVKLLSHGGDILELCHELRLPFLNWLVSCQKS 972

Query: 819 SFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHSDSCD 878
           SFKRYDI+CVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHS+SCD
Sbjct: 1070 SFKRYDIACVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHSESCD 972

Query: 879 IHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQ 906
           IHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQ
Sbjct: 1130 IHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQ 972

BLAST of Lag0001886 vs. TAIR 10
Match: AT3G59410.1 (protein kinase family protein )

HSP 1 Score: 848.6 bits (2191), Expect = 4.8e-246
Identity = 418/566 (73.85%), Postives = 472/566 (83.39%), Query Frame = 0

Query: 361 GHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAW 420
           G GGFGHVVLCKNKLDGRQYAVKKIRLKDK +PVN RI+REVATLSRLQHQHVVRYYQAW
Sbjct: 432 GQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVVRYYQAW 491

Query: 421 YETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQV 480
           +ETGV D +  A WGS T  SS FSY GA +T   E +N +ESTYLYIQMEYCPRTLRQV
Sbjct: 492 FETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPRTLRQV 551

Query: 481 FESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFL 540
           FESY HFDK+FAWHL  QIVEGLAHIHGQGIIHRD TP+NIFFDARNDIKIGDFGLAKFL
Sbjct: 552 FESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFGLAKFL 611

Query: 541 KLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELW 600
           KLEQLDQD G   D  G  +D TGQ GTYFYTAPEIEQ WPKIDEKADMYSLG+VFFELW
Sbjct: 612 KLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVVFFELW 671

Query: 601 HPFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFP 660
           HPFGTAMERH++L++LK KGELP  WV EF EQASLLRRLMS SPS+RPSA+ELL+HAFP
Sbjct: 672 HPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFP 731

Query: 661 PRMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHD----CGTAS-IQYTD 720
           PRME +LLDNILR M+TSEDSS+YD+VV+ IFDEE L MK  Q      C   S IQYT+
Sbjct: 732 PRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSHQSSRSRLCADDSYIQYTE 791

Query: 721 LGTEVRDHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHEL 780
           + TE+RD+V+++T+E+FR HCAKHLE+  M LL    Q +R TVKLL++GGD+LELC+EL
Sbjct: 792 INTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 851

Query: 781 RLPFLNWLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVT 840
           RLPF++W+  +QKSSFKRY+IS VYRRAIGHSPPN  LQ DFDI+GGT +LTEAEV+KV 
Sbjct: 852 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVI 911

Query: 841 VDVISYFFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSK 900
           VD+ ++ FH  SCDIHLNHGDLL+AIWSW G+KAEHR KVAELLSMM SLRPQSSERK K
Sbjct: 912 VDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLK 971

Query: 901 WVVIRRQLLQGESAVPHLDMHYLSVV 922
           WV IRRQLLQ E  +P   ++ L  V
Sbjct: 972 WVFIRRQLLQ-ELKLPEAVVNRLQTV 996

HSP 2 Score: 77.8 bits (190), Expect = 5.2e-14
Identity = 58/142 (40.85%), Postives = 72/142 (50.70%), Query Frame = 0

Query: 39  QDSILTDHVAEDEDHGYESEP-SEWSFASLSNEQESQTTDRDIMMV-------------- 98
           ++S L D  AED+    ESE    WS  SL+ +Q  Q + +D++MV              
Sbjct: 292 KESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLA 351

Query: 99  ---------------LSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN-PSDFGG 150
                          LS+  LDLASK S  F++ FE AF   M STS  QFW  PSD   
Sbjct: 352 DALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCE 411

BLAST of Lag0001886 vs. TAIR 10
Match: AT3G59410.2 (protein kinase family protein )

HSP 1 Score: 848.6 bits (2191), Expect = 4.8e-246
Identity = 418/566 (73.85%), Postives = 472/566 (83.39%), Query Frame = 0

Query: 361  GHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAW 420
            G GGFGHVVLCKNKLDGRQYAVKKIRLKDK +PVN RI+REVATLSRLQHQHVVRYYQAW
Sbjct: 456  GQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVVRYYQAW 515

Query: 421  YETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCPRTLRQV 480
            +ETGV D +  A WGS T  SS FSY GA +T   E +N +ESTYLYIQMEYCPRTLRQV
Sbjct: 516  FETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPRTLRQV 575

Query: 481  FESYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFL 540
            FESY HFDK+FAWHL  QIVEGLAHIHGQGIIHRD TP+NIFFDARNDIKIGDFGLAKFL
Sbjct: 576  FESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFGLAKFL 635

Query: 541  KLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELW 600
            KLEQLDQD G   D  G  +D TGQ GTYFYTAPEIEQ WPKIDEKADMYSLG+VFFELW
Sbjct: 636  KLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVVFFELW 695

Query: 601  HPFGTAMERHLVLSDLKQKGELPTTWVAEFSEQASLLRRLMSQSPSERPSASELLQHAFP 660
            HPFGTAMERH++L++LK KGELP  WV EF EQASLLRRLMS SPS+RPSA+ELL+HAFP
Sbjct: 696  HPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFP 755

Query: 661  PRMEYQLLDNILRTMKTSEDSSIYDKVVNAIFDEESLTMKDDQHD----CGTAS-IQYTD 720
            PRME +LLDNILR M+TSEDSS+YD+VV+ IFDEE L MK  Q      C   S IQYT+
Sbjct: 756  PRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSHQSSRSRLCADDSYIQYTE 815

Query: 721  LGTEVRDHVIDVTREMFRLHCAKHLEISSMYLLDGSMQINRNTVKLLSHGGDILELCHEL 780
            + TE+RD+V+++T+E+FR HCAKHLE+  M LL    Q +R TVKLL++GGD+LELC+EL
Sbjct: 816  INTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 875

Query: 781  RLPFLNWLVSSQKSSFKRYDISCVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVT 840
            RLPF++W+  +QKSSFKRY+IS VYRRAIGHSPPN  LQ DFDI+GGT +LTEAEV+KV 
Sbjct: 876  RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVI 935

Query: 841  VDVISYFFHSDSCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSK 900
            VD+ ++ FH  SCDIHLNHGDLL+AIWSW G+KAEHR KVAELLSMM SLRPQSSERK K
Sbjct: 936  VDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLK 995

Query: 901  WVVIRRQLLQGESAVPHLDMHYLSVV 922
            WV IRRQLLQ E  +P   ++ L  V
Sbjct: 996  WVFIRRQLLQ-ELKLPEAVVNRLQTV 1020

HSP 2 Score: 77.8 bits (190), Expect = 5.2e-14
Identity = 58/142 (40.85%), Postives = 72/142 (50.70%), Query Frame = 0

Query: 39  QDSILTDHVAEDEDHGYESEP-SEWSFASLSNEQESQTTDRDIMMV-------------- 98
           ++S L D  AED+    ESE    WS  SL+ +Q  Q + +D++MV              
Sbjct: 316 KESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLA 375

Query: 99  ---------------LSKVALDLASKPSSMFHKKFETAFRDQMNSTSFSQFWN-PSDFGG 150
                          LS+  LDLASK S  F++ FE AF   M STS  QFW  PSD   
Sbjct: 376 DALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCE 435

BLAST of Lag0001886 vs. TAIR 10
Match: AT1G54510.1 (NIMA-related serine/threonine kinase 1 )

HSP 1 Score: 103.6 bits (257), Expect = 8.8e-22
Identity = 84/320 (26.25%), Postives = 142/320 (44.38%), Query Frame = 0

Query: 361 GHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAW 420
           G G FG  +L ++K + ++Y +KKIRL  +         +E+  +S+++H  +V Y  +W
Sbjct: 11  GKGSFGSALLVRHKHEKKKYVLKKIRLARQTQRTRRSAHQEMELISKMRHPFIVEYKDSW 70

Query: 421 YETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCP-RTLRQ 480
            E                                       ++ Y+ I + YC    + Q
Sbjct: 71  VE---------------------------------------KACYVCIVIGYCEGGDMAQ 130

Query: 481 VFE--SYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLA 540
             +  +  HF +E       Q++ GL ++H   I+HRD+  SNIF     DI++GDFGLA
Sbjct: 131 AIKKSNGVHFQEEKLCKWLVQLLMGLEYLHSNHILHRDVKCSNIFLTKEQDIRLGDFGLA 190

Query: 541 KFLKLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFF 600
           K L  + L   V                VGT  Y  PE+    P    K+D++SLG   +
Sbjct: 191 KILTSDDLTSSV----------------VGTPSYMCPELLADIP-YGSKSDIWSLGCCIY 250

Query: 601 ELWH--PFGTAMERHLVLSDLKQK--GELPTTWVAEFSEQASLLRRLMSQSPSERPSASE 660
           E+ +  P   A +   +++ + +     LP  +   F     L++ ++ ++P  RPSAS+
Sbjct: 251 EMAYLKPAFKAFDMQALINKINKTIVSPLPAKYSGPF---RGLVKSMLRKNPEVRPSASD 268

Query: 661 LLQHAFPPRMEYQLLDNILR 674
           LL+H   P ++  +LD  LR
Sbjct: 311 LLRH---PHLQPYVLDVKLR 268

BLAST of Lag0001886 vs. TAIR 10
Match: AT1G54510.2 (NIMA-related serine/threonine kinase 1 )

HSP 1 Score: 103.6 bits (257), Expect = 8.8e-22
Identity = 84/320 (26.25%), Postives = 142/320 (44.38%), Query Frame = 0

Query: 361 GHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAW 420
           G G FG  +L ++K + ++Y +KKIRL  +         +E+  +S+++H  +V Y  +W
Sbjct: 11  GKGSFGSALLVRHKHEKKKYVLKKIRLARQTQRTRRSAHQEMELISKMRHPFIVEYKDSW 70

Query: 421 YETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCP-RTLRQ 480
            E                                       ++ Y+ I + YC    + Q
Sbjct: 71  VE---------------------------------------KACYVCIVIGYCEGGDMAQ 130

Query: 481 VFE--SYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLA 540
             +  +  HF +E       Q++ GL ++H   I+HRD+  SNIF     DI++GDFGLA
Sbjct: 131 AIKKSNGVHFQEEKLCKWLVQLLMGLEYLHSNHILHRDVKCSNIFLTKEQDIRLGDFGLA 190

Query: 541 KFLKLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFF 600
           K L  + L   V                VGT  Y  PE+    P    K+D++SLG   +
Sbjct: 191 KILTSDDLTSSV----------------VGTPSYMCPELLADIP-YGSKSDIWSLGCCIY 250

Query: 601 ELWH--PFGTAMERHLVLSDLKQK--GELPTTWVAEFSEQASLLRRLMSQSPSERPSASE 660
           E+ +  P   A +   +++ + +     LP  +   F     L++ ++ ++P  RPSAS+
Sbjct: 251 EMAYLKPAFKAFDMQALINKINKTIVSPLPAKYSGPF---RGLVKSMLRKNPEVRPSASD 268

Query: 661 LLQHAFPPRMEYQLLDNILR 674
           LL+H   P ++  +LD  LR
Sbjct: 311 LLRH---PHLQPYVLDVKLR 268

BLAST of Lag0001886 vs. TAIR 10
Match: AT1G54510.3 (NIMA-related serine/threonine kinase 1 )

HSP 1 Score: 103.6 bits (257), Expect = 8.8e-22
Identity = 84/320 (26.25%), Postives = 142/320 (44.38%), Query Frame = 0

Query: 361 GHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAW 420
           G G FG  +L ++K + ++Y +KKIRL  +         +E+  +S+++H  +V Y  +W
Sbjct: 11  GKGSFGSALLVRHKHEKKKYVLKKIRLARQTQRTRRSAHQEMELISKMRHPFIVEYKDSW 70

Query: 421 YETGVSDSYGEAAWGSMTPLSSTFSYKGASATGTLEHENKVESTYLYIQMEYCP-RTLRQ 480
            E                                       ++ Y+ I + YC    + Q
Sbjct: 71  VE---------------------------------------KACYVCIVIGYCEGGDMAQ 130

Query: 481 VFE--SYTHFDKEFAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLA 540
             +  +  HF +E       Q++ GL ++H   I+HRD+  SNIF     DI++GDFGLA
Sbjct: 131 AIKKSNGVHFQEEKLCKWLVQLLMGLEYLHSNHILHRDVKCSNIFLTKEQDIRLGDFGLA 190

Query: 541 KFLKLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFF 600
           K L  + L   V                VGT  Y  PE+    P    K+D++SLG   +
Sbjct: 191 KILTSDDLTSSV----------------VGTPSYMCPELLADIP-YGSKSDIWSLGCCIY 250

Query: 601 ELWH--PFGTAMERHLVLSDLKQK--GELPTTWVAEFSEQASLLRRLMSQSPSERPSASE 660
           E+ +  P   A +   +++ + +     LP  +   F     L++ ++ ++P  RPSAS+
Sbjct: 251 EMAYLKPAFKAFDMQALINKINKTIVSPLPAKYSGPF---RGLVKSMLRKNPEVRPSASD 268

Query: 661 LLQHAFPPRMEYQLLDNILR 674
           LL+H   P ++  +LD  LR
Sbjct: 311 LLRH---PHLQPYVLDVKLR 268