Lag0001853 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0001853
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Biotin synthase
Location	chr4: 36227543 .. 36231065 (-)
RNA-Seq Expression	Lag0001853
Synteny	Lag0001853

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTCTCAAAGCTTCCTCCATGAAAATTATGAGAAGGTCGTCTTTATTTCAGGTTCTATTGCACAGTCTTTGTAAGTAAAGCTTCTCATTAATTTTCATGGAGGAATACTTTGAAGGAAGAGAGAGGAAGAAGAAAAAGAAACGTTTTTCATCTAAAACGTTATTTTTGAAATTTTTTCCCAAGTCATGGGTTAATGCAACTTGTTAAAGTTGAGGTATTATTGAACTTTGTGATCGACATAAGAGGGATGAGAGAAGGAAGCATTTCATTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGAGGGATTCAGGTACTCTCCTTACCATATATTGATATATTGAGGAAGATAATGCCCTAAGGCAATACAAAACATTCTTACAGTCGATTTGAGAGGGCCGGTAATCTCTCCCCTTACCCTAGAAAAGCATCAAATTCCACAGTACTAACCTATTACATACATATAGTTCCCAACAACCCAAACATTCTCACTGCATTCCCCTCTCCTCCCTGACCTTTCTAACTAACTGATCGACGGGGAAATCCCTTTCTTACCCCTCTGTGTATATTGAATGGTAATTGGGGGCCTAATAGATTCTTTTTGTTTTAAACTTTTGGTGTAAATGATATAAAGCCTTTTTTTTTTTGCTAATTGGTAGATTTTGTGTAACTTTCTTATGACTTTCATATTTCTCATTCTATTGATGACGTCATTTTTACAAAAAAAAAAGGTTGTTACATGGCGATGTCTTCACTTTTTCTCCTCTCTTCTTCATTCCATGCTATCCTCTTATTGCACATTATCTGTACCTTGAGCACCATTTAAAAGCATAATTCACATTGTCTGGCAGGCAAAAGAGGCTGGTAGTACAAGATTTTGTATGGGTGCTGCGTGGAGGGACACGATAGGAAGAAAAACTAATTTCAATCAGATACTTGATTATGTAAAAGAAATAAGGTAACGAATTTTCACCTTAAGTAACAATTAGCTACAAAGATAGAAGACCCAAATAAGTAGTTTACTTACAGAATACTGATTAAAAATGAATCAACAGTAAAATGAAAAAAAGTTTTTATATTCCCCTGCTCCTGTCAGGTTAGAATTGTTAGGACGAAAATGCTGTTGCATCTTAGAGTGAGTGATGGCACATAGCAGAGCACTTGGTAAGAATCTGATTTAAAAATAAACATTTAGTCAATGATTTGAAAGCGCCCAGTCTTGCACTTTTTTTTTTTTTTTTTTTTTGATAGGAAACAAGAGCCATTTCATTGACGTATGAAATAGATACAAAAGGGAGTCTATGTCTTGCACTTGTGTTTTCTCCCCCTTCTTTTCTTTACATACTAGAAAGAAAGTTGCTAGGATTTCTACTAAACACAGTTTCTTAAGCAAAGCCTTTGTCTTTTCTAATTTTTTTTAATCTCCACTTTATATTATGAAATTTTGTACTCTTTCAAGGCTTGGAATTTAGTTTTCTTCCCCTTTTGGTAATTTTTGCAATCAATGAAATATTTTTCTTATATATATATGTAAGATTTCTGCAATTGTTAAGATTAGCAATGGTTGACTCTTTAATATTGTCCAATCAAGCTATTGAAATTCATGATTGAGAAACTGAAACTTTGGTGAGAATGTACCACAATTATAGCTCTTTTGAATGAGAATAATCACTTGTCCATCGACATTCATTCTCAAATCAAAATTTTCTGTGATGTATTAGAGAGATGGGGATGGAGGTCTGCTGCACCTTGGGCATGATAGAGAAGCAACAAGCTGTAGAACTCAAGAAGGCAGGCCTTACAGCTTATAACCACAACCTAGACACGTCAAGGGAATACTACCCAAATATTATTACCACGAGGAGTTATGATGAGCGTTTGAAAACCCTAGAATTTGTTCGTGATGCTGGAATCAATGTCTGTTCTGGTAATTTTGTACATCTTTGATTTTGACTAAGATTTGGATTGATATATTGATAATTTGATTGGATATTCATAGATTCATAACTTATTTATGGGAAAAAGTTTCATTGAGAATAACTACTAGAAGAAATAGCTTACGAAAGAGAGGGGAAAAGCCCCACAAATCATAAGACTACAGAAGGGCAAACCAGTTGGCATTTGGCCATTATTTAGAATTCCTAACGCATGTAAGGAAAAATATATTCCTGGAAAATGTTCAGCCTGGTCAGAAAGCCAAGAAGTCTACTAAAAGTTATAATTGTAACAATTTGAAGAGGATAATATTCATTCCAGTGAGACATAGATATGGCTTTAAGATGGTATGAGGTTTCATCTGTTAGTAGACCAACTAACCCAAGGGTTGGGATCTGAGGACGTTGCATTTCTGAAGATCTCCACAGCTTCCGGGTTCTTGATTTGGGGTATTCGCTTGCCTGATGATGTTGGGAACATTTTTATTGATTATTTGTGCTGTGATTTTGTCTAGTTCTCTAATGTCCTCTTTATGATGGTAAACATCCAAAGAGATTTTTCCCCTTTCGTTGGTTGCTAGATGGTGGCGTAAACTCTCCTTTTTTTTTAATAGAAAAATATATATTTTTTGATGTGATGAAATATACCAAAGAAAGTTTCTGGGAGCCTTTGTAGTCTTACTTTGATATTCAAGCCAATTCTAGAGGTTTTTGCAACACCTTTGGCTAGATTATGAAGTTACCTCTTCCACCCCTTTTGTACTCTCTTTTTATGGCACATTATTGCAGTGTTTCTCCTTTGAAGAATATAAAGGGTATTAGGTAGACAAATACTCTCTGGGATTAATCCAGTAATGACTTCCTTTGAAAGCTAAACGTTTCTCTTTAATTTTTTAGCATTTTGAAGTAAATATGGCATTGGTACTTATGAGAACTTGGTGAAATTCTTAATTTATGGCAATAGGAGGAATAATTGGGCTTGGAGAGGCAGAAGAAGACCGTGTCGGTTTGCTGCATACCTTGGCAACGCTACCCACGCACCCTGAGAGCGTTCCCATCAACGCGCTGGTTGCAGTGAAAGGAACCCCTCTTCAAGATCAAAAAGTAGCTCCTATTTACTTAAGAATAACCTTTTTGTTTATTGCTCTCGAAGTCAGTTTACACGCTTGTTCATATTTGTTGCACAATTACCTTGTCTTATCTAGACACCACAACTTTCTTACTCATGAAATCTAGTTGCTTTGATTTCTATAGTGAATGATTTTCTTACCTATCTTTTCAGCCTGTTGAAATATGGGAGATGGTTCGAATGATCGCTACAGCACGGATCACCATGCCGAAAGCAATGGTCAGGTTGTCAGCAGGTAGAGTGAAGTTCTCCATGCCCGAGCAAGCGTTATGCTTCCTTGCAGGAGCGAATTCTATCTTCACCGGAGAGAAACTGCTAACGACGCCAAATAATGATTTTGATGCTGATCAACTCATGTTCAAGATGCTAGGTCTGATTCCAAAAGCTCCCAGTTTCACTGATGATGTTGAGAAAGCCCCTGAAGAAGAGTCATTACAAGAAGCTGCTTCCAGTTCATAA

mRNA sequence

ATGAAGTCTCAAAGCTTCCTCCATGAAAATTATGAGAAGGTCGTCTTTATTTCAGGTTCTATTGCACAGTCTTTTCGATTTGAGAGGGCCGGTAATCTCTCCCCTTACCCTAGAAAAGCATCAAATTCCACAGCAAAAGAGGCTGGTAGTACAAGATTTTGTATGGGTGCTGCGTGGAGGGACACGATAGGAAGAAAAACTAATTTCAATCAGATACTTGATTATGTAAAAGAAATAAGAGAGATGGGGATGGAGGTCTGCTGCACCTTGGGCATGATAGAGAAGCAACAAGCTGTAGAACTCAAGAAGGCAGGCCTTACAGCTTATAACCACAACCTAGACACGTCAAGGGAATACTACCCAAATATTATTACCACGAGGAGTTATGATGAGCGTTTGAAAACCCTAGAATTTGTTCGTGATGCTGGAATCAATGTCTGTTCTGGAGGAATAATTGGGCTTGGAGAGGCAGAAGAAGACCGTGTCGGTTTGCTGCATACCTTGGCAACGCTACCCACGCACCCTGAGAGCGTTCCCATCAACGCGCTGGTTGCAGTGAAAGGAACCCCTCTTCAAGATCAAAAACCTGTTGAAATATGGGAGATGGTTCGAATGATCGCTACAGCACGGATCACCATGCCGAAAGCAATGGTCAGGTTGTCAGCAGGTAGAGTGAAGTTCTCCATGCCCGAGCAAGCGTTATGCTTCCTTGCAGGAGCGAATTCTATCTTCACCGGAGAGAAACTGCTAACGACGCCAAATAATGATTTTGATGCTGATCAACTCATGTTCAAGATGCTAGGTCTGATTCCAAAAGCTCCCAGTTTCACTGATGATGTTGAGAAAGCCCCTGAAGAAGAGTCATTACAAGAAGCTGCTTCCAGTTCATAA

Coding sequence (CDS)

ATGAAGTCTCAAAGCTTCCTCCATGAAAATTATGAGAAGGTCGTCTTTATTTCAGGTTCTATTGCACAGTCTTTTCGATTTGAGAGGGCCGGTAATCTCTCCCCTTACCCTAGAAAAGCATCAAATTCCACAGCAAAAGAGGCTGGTAGTACAAGATTTTGTATGGGTGCTGCGTGGAGGGACACGATAGGAAGAAAAACTAATTTCAATCAGATACTTGATTATGTAAAAGAAATAAGAGAGATGGGGATGGAGGTCTGCTGCACCTTGGGCATGATAGAGAAGCAACAAGCTGTAGAACTCAAGAAGGCAGGCCTTACAGCTTATAACCACAACCTAGACACGTCAAGGGAATACTACCCAAATATTATTACCACGAGGAGTTATGATGAGCGTTTGAAAACCCTAGAATTTGTTCGTGATGCTGGAATCAATGTCTGTTCTGGAGGAATAATTGGGCTTGGAGAGGCAGAAGAAGACCGTGTCGGTTTGCTGCATACCTTGGCAACGCTACCCACGCACCCTGAGAGCGTTCCCATCAACGCGCTGGTTGCAGTGAAAGGAACCCCTCTTCAAGATCAAAAACCTGTTGAAATATGGGAGATGGTTCGAATGATCGCTACAGCACGGATCACCATGCCGAAAGCAATGGTCAGGTTGTCAGCAGGTAGAGTGAAGTTCTCCATGCCCGAGCAAGCGTTATGCTTCCTTGCAGGAGCGAATTCTATCTTCACCGGAGAGAAACTGCTAACGACGCCAAATAATGATTTTGATGCTGATCAACTCATGTTCAAGATGCTAGGTCTGATTCCAAAAGCTCCCAGTTTCACTGATGATGTTGAGAAAGCCCCTGAAGAAGAGTCATTACAAGAAGCTGCTTCCAGTTCATAA

Protein sequence

MKSQSFLHENYEKVVFISGSIAQSFRFERAGNLSPYPRKASNSTAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGRVKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAPEEESLQEAASSS

Homology

[Close]

BLAST of Lag0001853 vs. NCBI nr
Match: XP_038886510.1 (biotin synthase, mitochondrial [Benincasa hispida])

HSP 1 Score: 491.5 bits (1264), Expect = 5.1e-135
Identity = 245/252 (97.22%), Postives = 251/252 (99.60%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA++LKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFT+DV+KAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVQKAP 373

Query: 285 EEESLQEAASSS 297
           EEESLQE ASSS
Sbjct: 374 EEESLQEVASSS 385

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BLAST of Lag0001853 vs. NCBI nr
Match: XP_008445703.1 (PREDICTED: biotin synthase [Cucumis melo])

HSP 1 Score: 490.3 bits (1261), Expect = 1.1e-134
Identity = 244/250 (97.60%), Postives = 249/250 (99.60%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA++LKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFT+D EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 373

Query: 285 EEESLQEAAS 295
           EEESLQEAAS
Sbjct: 374 EEESLQEAAS 383

[Close]

BLAST of Lag0001853 vs. NCBI nr
Match: XP_004140874.1 (biotin synthase, mitochondrial [Cucumis sativus] >KGN45987.1 hypothetical protein Csa_005313 [Cucumis sativus])

HSP 1 Score: 490.3 bits (1261), Expect = 1.1e-134
Identity = 245/252 (97.22%), Postives = 250/252 (99.21%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA++LKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFT+D EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 373

Query: 285 EEESLQEAASSS 297
           EEESLQEAA SS
Sbjct: 374 EEESLQEAALSS 385

[Close]

BLAST of Lag0001853 vs. NCBI nr
Match: KAA0033098.1 (biotin synthase [Cucumis melo var. makuwa] >TYK17135.1 biotin synthase [Cucumis melo var. makuwa])

HSP 1 Score: 490.3 bits (1261), Expect = 1.1e-134
Identity = 244/250 (97.60%), Postives = 249/250 (99.60%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA++LKKA
Sbjct: 140 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 199

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 200 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 259

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 260 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 319

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFT+D EKAP
Sbjct: 320 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 379

Query: 285 EEESLQEAAS 295
           EEESLQEAAS
Sbjct: 380 EEESLQEAAS 389

[Close]

BLAST of Lag0001853 vs. NCBI nr
Match: XP_022150359.1 (biotin synthase [Momordica charantia])

HSP 1 Score: 488.0 bits (1255), Expect = 5.6e-134
Identity = 245/252 (97.22%), Postives = 250/252 (99.21%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA ELKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAEELKKA 193

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLP+HPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPSHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSF+D VEKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFSDGVEKAP 373

Query: 285 EEESLQEAASSS 297
           EEESLQEAAS+S
Sbjct: 374 EEESLQEAASNS 385

[Close]

BLAST of Lag0001853 vs. ExPASy Swiss-Prot
Match: P54967 (Biotin synthase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BIO2 PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 8.8e-122
Identity = 217/252 (86.11%), Postives = 236/252 (93.65%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNF+QIL+Y+KEIR MGMEVCCTLGMIEKQQA+ELKKA
Sbjct: 129 AKEAGSTRFCMGAAWRDTIGRKTNFSQILEYIKEIRGMGMEVCCTLGMIEKQQALELKKA 188

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPN+ITTRSYD+RL+TL  VRDAGINVCSGGIIGLGEAEEDR+GL
Sbjct: 189 GLTAYNHNLDTSREYYPNVITTRSYDDRLETLSHVRDAGINVCSGGIIGLGEAEEDRIGL 248

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLP+HPESVPINAL+AVKGTPL+DQKPVEIWEM+RMI TARI MPKAMVRLSAGR
Sbjct: 249 LHTLATLPSHPESVPINALLAVKGTPLEDQKPVEIWEMIRMIGTARIVMPKAMVRLSAGR 308

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           V+FSM EQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFK LGLIPK PSF++D     
Sbjct: 309 VRFSMSEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKTLGLIPKPPSFSED---DS 368

Query: 285 EEESLQEAASSS 297
           E E+ ++ AS+S
Sbjct: 369 ESENCEKVASAS 377

[Close]

BLAST of Lag0001853 vs. ExPASy Swiss-Prot
Match: Q11S94 (Biotin synthase OS=Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465) OX=269798 GN=bioB PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.9e-84
Identity = 157/243 (64.61%), Postives = 194/243 (79.84%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKE+GSTRFCMGAAWR+    K +F++++D VK +  MGMEVCCTLGM+  +QA +LK A
Sbjct: 89  AKESGSTRFCMGAAWREVRDNK-DFDKVIDMVKGVSTMGMEVCCTLGMLTPEQADKLKDA 148

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GL AYNHNLDTS E+Y  +ITTR+YD+RL+TL+ VR+A I+VCSGGIIG+GE+  DRVG+
Sbjct: 149 GLYAYNHNLDTSAEHYDKVITTRTYDDRLETLDNVRNAKISVCSGGIIGMGESHGDRVGM 208

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLA +  HPESVP+NALV V+GTPL+DQ  V +WEMVRMIATARI MPKAMVRLSAGR
Sbjct: 209 LHTLANMVEHPESVPVNALVPVEGTPLEDQPRVSVWEMVRMIATARIIMPKAMVRLSAGR 268

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           V+ +  EQALCFLAGANSIF G+KLLTTPN + +AD+ MF++L L P+      D  K  
Sbjct: 269 VRMNTEEQALCFLAGANSIFAGDKLLTTPNPEVNADKEMFQVLNLKPRQSFKNGDAPKIK 328

Query: 285 EEE 288
            E+
Sbjct: 329 FEQ 330

[Close]

BLAST of Lag0001853 vs. ExPASy Swiss-Prot
Match: Q5AYI7 (Biotin synthase, mitochondrial OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bioB PE=2 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.4e-82
Identity = 158/279 (56.63%), Postives = 199/279 (71.33%), Query Frame = 0


Query: 22  AQSFRFE---RAGNLSPYPRKASNS-TAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVK 81
           AQS R+    +A  +SP       +  AK  GSTRFCMGAAWRD  GRKT+   +   V 
Sbjct: 104 AQSSRYSTGLKATKMSPVDDVLEKARIAKANGSTRFCMGAAWRDMRGRKTSLKNVKQMVS 163

Query: 82  EIREMGMEVCCTLGMIEKQQAVELKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLE 141
            +REMGMEVC TLGMI+  QA ELK AGLTAYNHNLDTSRE+YP IITTRSYDERLKTL 
Sbjct: 164 GVREMGMEVCVTLGMIDADQAKELKDAGLTAYNHNLDTSREFYPTIITTRSYDERLKTLS 223

Query: 142 FVRDAGINVCSGGIIGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPV 201
            VRDAGINVCSGGI+GLGEA+ DR+GL+HT+++LP+HPES P+NALV +KGTPL D+K +
Sbjct: 224 HVRDAGINVCSGGILGLGEADSDRIGLIHTVSSLPSHPESFPVNALVPIKGTPLGDRKMI 283

Query: 202 EIWEMVRMIATARITMPKAMVRLSAGRVKFSMPEQALCFLAGANSIFTGEKLLTTPNNDF 261
              +++R +ATARI +P  +VRL+AGR+  +  +Q  CF+AGAN++FTGEK+LTT  N +
Sbjct: 284 SFDKLLRTVATARIVLPATIVRLAAGRISLTEEQQVACFMAGANAVFTGEKMLTTDCNGW 343

Query: 262 DADQLMFKMLGLIPKAPSFTDDVEKAPEEESLQEAASSS 297
           D D+ MF   G  P   SF  +   A  ++ +   A  S
Sbjct: 344 DEDRAMFDRWGFYPMR-SFEKETNAATPQQHVDSVAHES 381

[Close]

BLAST of Lag0001853 vs. ExPASy Swiss-Prot
Match: A5FLT1 (Biotin synthase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=bioB PE=3 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.2e-75
Identity = 138/236 (58.47%), Postives = 178/236 (75.42%), Query Frame = 0


Query: 39  KASNSTAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA 98
           KA    AK  GS+R CMGAAWR+ +     F+Q+L+ V+ I ++ MEVCCTLGM+ + QA
Sbjct: 84  KAQALRAKSNGSSRVCMGAAWRN-VKDGEEFDQVLEMVRTINKLDMEVCCTLGMLTENQA 143

Query: 99  VELKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAE 158
             L +AGL AYNHNLDTS EYY ++I+TR +++RL+T+E VR   + VCSGGIIG+GE+ 
Sbjct: 144 QRLAEAGLYAYNHNLDTSEEYYKDVISTRGFEDRLQTIENVRKTNVTVCSGGIIGMGESI 203

Query: 159 EDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMV 218
           EDR G+L  L+TL   PESVPINALVAV+GTP++++KPVEIWEM+RM+AT RI MP+  V
Sbjct: 204 EDRAGMLVALSTLNPQPESVPINALVAVEGTPMEEEKPVEIWEMIRMVATTRIVMPETQV 263

Query: 219 RLSAGRVKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAP 275
           RLSAGR   S   QA+CF AGANSIF G+KLLTTPN D + D  MF+ LG++ + P
Sbjct: 264 RLSAGRTNMSREGQAMCFFAGANSIFAGDKLLTTPNPDVNEDMKMFETLGMVAQKP 318

[Close]

BLAST of Lag0001853 vs. ExPASy Swiss-Prot
Match: A6GW77 (Biotin synthase OS=Flavobacterium psychrophilum (strain ATCC 49511 / DSM 21280 / CIP 103535 / JIP02/86) OX=402612 GN=bioB PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.6e-75
Identity = 138/236 (58.47%), Postives = 178/236 (75.42%), Query Frame = 0


Query: 39  KASNSTAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA 98
           KA    AK +GS+R CMGAAWR+ +     F+Q+L+ V+ I ++ MEVCCTLGMI + QA
Sbjct: 83  KAQALRAKSSGSSRVCMGAAWRN-VKDGPEFDQVLEMVRTINKLDMEVCCTLGMITENQA 142

Query: 99  VELKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAE 158
             L +AGL AYNHNLDTS EYY  +I+TR +++R++T+  VR   + VCSGGIIG+GE+ 
Sbjct: 143 HRLAEAGLYAYNHNLDTSEEYYKEVISTRGFEDRIETIANVRKTNVTVCSGGIIGMGESI 202

Query: 159 EDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMV 218
            DR G+L  L+TL   PESVPINALVAV+GTP++++KPVEIWEM+RM+AT RI MP+  V
Sbjct: 203 GDRAGMLVALSTLSPQPESVPINALVAVEGTPMEEEKPVEIWEMIRMVATTRIIMPETQV 262

Query: 219 RLSAGRVKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAP 275
           RLSAGR+  +   QA+CF AGANSIF G+KLLTTPN D + D  MF+MLGL P+ P
Sbjct: 263 RLSAGRMNMTREGQAMCFFAGANSIFAGDKLLTTPNPDVNEDMKMFEMLGLNPQKP 317

[Close]

BLAST of Lag0001853 vs. ExPASy TrEMBL
Match: A0A5D3D025 (Biotin synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G00740 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.5e-135
Identity = 244/250 (97.60%), Postives = 249/250 (99.60%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA++LKKA
Sbjct: 140 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 199

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 200 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 259

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 260 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 319

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFT+D EKAP
Sbjct: 320 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 379

Query: 285 EEESLQEAAS 295
           EEESLQEAAS
Sbjct: 380 EEESLQEAAS 389

[Close]

BLAST of Lag0001853 vs. ExPASy TrEMBL
Match: A0A0A0KCA2 (Biotin synthase OS=Cucumis sativus OX=3659 GN=Csa_6G041720 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.5e-135
Identity = 245/252 (97.22%), Postives = 250/252 (99.21%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA++LKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFT+D EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 373

Query: 285 EEESLQEAASSS 297
           EEESLQEAA SS
Sbjct: 374 EEESLQEAALSS 385

[Close]

BLAST of Lag0001853 vs. ExPASy TrEMBL
Match: A0A1S3BDC5 (Biotin synthase OS=Cucumis melo OX=3656 GN=LOC103488656 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.5e-135
Identity = 244/250 (97.60%), Postives = 249/250 (99.60%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA++LKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFT+D EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 373

Query: 285 EEESLQEAAS 295
           EEESLQEAAS
Sbjct: 374 EEESLQEAAS 383

[Close]

BLAST of Lag0001853 vs. ExPASy TrEMBL
Match: A0A6J1D9U9 (Biotin synthase OS=Momordica charantia OX=3673 GN=LOC111018547 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.7e-134
Identity = 245/252 (97.22%), Postives = 250/252 (99.21%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA ELKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAEELKKA 193

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLP+HPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPSHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSF+D VEKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFSDGVEKAP 373

Query: 285 EEESLQEAASSS 297
           EEESLQEAAS+S
Sbjct: 374 EEESLQEAASNS 385

[Close]

BLAST of Lag0001853 vs. ExPASy TrEMBL
Match: A0A6J1EPY7 (Biotin synthase OS=Cucurbita moschata OX=3662 GN=LOC111436330 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.3e-133
Identity = 242/251 (96.41%), Postives = 248/251 (98.80%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVK+IREMGMEVCCTLGMIEKQQA++LKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEM+RMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           VKFS+PEQALCFLAGANSIFTGEKLLTTPNNDFDADQ MFKMLGLIPKAPSFTD  EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIPKAPSFTDGAEKAP 373

Query: 285 EEESLQEAASS 296
           EEESLQEAAS+
Sbjct: 374 EEESLQEAASN 384

[Close]

BLAST of Lag0001853 vs. TAIR 10
Match: AT2G43360.1 (Radical SAM superfamily protein )

HSP 1 Score: 438.0 bits (1125), Expect = 6.2e-123
Identity = 217/252 (86.11%), Postives = 236/252 (93.65%), Query Frame = 0


Query: 45  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAVELKKA 104
           AKEAGSTRFCMGAAWRDTIGRKTNF+QIL+Y+KEIR MGMEVCCTLGMIEKQQA+ELKKA
Sbjct: 129 AKEAGSTRFCMGAAWRDTIGRKTNFSQILEYIKEIRGMGMEVCCTLGMIEKQQALELKKA 188

Query: 105 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 164
           GLTAYNHNLDTSREYYPN+ITTRSYD+RL+TL  VRDAGINVCSGGIIGLGEAEEDR+GL
Sbjct: 189 GLTAYNHNLDTSREYYPNVITTRSYDDRLETLSHVRDAGINVCSGGIIGLGEAEEDRIGL 248

Query: 165 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMVRMIATARITMPKAMVRLSAGR 224
           LHTLATLP+HPESVPINAL+AVKGTPL+DQKPVEIWEM+RMI TARI MPKAMVRLSAGR
Sbjct: 249 LHTLATLPSHPESVPINALLAVKGTPLEDQKPVEIWEMIRMIGTARIVMPKAMVRLSAGR 308

Query: 225 VKFSMPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTDDVEKAP 284
           V+FSM EQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFK LGLIPK PSF++D     
Sbjct: 309 VRFSMSEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKTLGLIPKPPSFSED---DS 368

Query: 285 EEESLQEAASSS 297
           E E+ ++ AS+S
Sbjct: 369 ESENCEKVASAS 377

The following BLAST results are available for this feature:

BLAST of Lag0001853 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886510.1	5.1e-135	97.22	biotin synthase, mitochondrial [Benincasa hispida]	[more]
XP_008445703.1	1.1e-134	97.60	PREDICTED: biotin synthase [Cucumis melo]	[more]
XP_004140874.1	1.1e-134	97.22	biotin synthase, mitochondrial [Cucumis sativus] >KGN45987.1 hypothetical protei...	[more]
KAA0033098.1	1.1e-134	97.60	biotin synthase [Cucumis melo var. makuwa] >TYK17135.1 biotin synthase [Cucumis ...	[more]
XP_022150359.1	5.6e-134	97.22	biotin synthase [Momordica charantia]	[more]

BLAST of Lag0001853 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P54967	8.8e-122	86.11	Biotin synthase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BIO2 PE=1 SV=1	[more]
Q11S94	1.9e-84	64.61	Biotin synthase OS=Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 10...	[more]
Q5AYI7	1.4e-82	56.63	Biotin synthase, mitochondrial OS=Emericella nidulans (strain FGSC A4 / ATCC 381...	[more]
A5FLT1	1.2e-75	58.47	Biotin synthase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM...	[more]
A6GW77	3.6e-75	58.47	Biotin synthase OS=Flavobacterium psychrophilum (strain ATCC 49511 / DSM 21280 /...	[more]

BLAST of Lag0001853 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D025	5.5e-135	97.60	Biotin synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G007...	[more]
A0A0A0KCA2	5.5e-135	97.22	Biotin synthase OS=Cucumis sativus OX=3659 GN=Csa_6G041720 PE=3 SV=1	[more]
A0A1S3BDC5	5.5e-135	97.60	Biotin synthase OS=Cucumis melo OX=3656 GN=LOC103488656 PE=3 SV=1	[more]
A0A6J1D9U9	2.7e-134	97.22	Biotin synthase OS=Momordica charantia OX=3673 GN=LOC111018547 PE=3 SV=1	[more]
A0A6J1EPY7	2.3e-133	96.41	Biotin synthase OS=Cucurbita moschata OX=3662 GN=LOC111436330 PE=3 SV=1	[more]

BLAST of Lag0001853 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43360.1	6.2e-123	86.11	Radical SAM superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010722	Biotin and thiamin synthesis-associated domain	SMART	SM00876	BATS_2	coord: 179..271 e-value: 2.0E-41 score: 153.6
IPR010722	Biotin and thiamin synthesis-associated domain	PFAM	PF06968	BATS	coord: 179..269 e-value: 2.5E-18 score: 66.0
IPR006638	Elp3/MiaB/NifB	SMART	SM00729	MiaB	coord: 16..208 e-value: 2.8E-21 score: 86.7
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 25..273 e-value: 2.7E-101 score: 340.9
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 71..165 e-value: 4.9E-7 score: 30.3
IPR007197	Radical SAM	PROSITE	PS51918	RADICAL_SAM	coord: 1..224 score: 23.668663
IPR002684	Biotin synthase/Biotin biosynthesis bifunctional protein BioAB	TIGRFAM	TIGR00433	TIGR00433	coord: 42..269 e-value: 1.6E-87 score: 291.5
IPR002684	Biotin synthase/Biotin biosynthesis bifunctional protein BioAB	PANTHER	PTHR22976	BIOTIN SYNTHASE	coord: 43..288
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 276..296
None	No IPR available	CDD	cd01335	Radical_SAM	coord: 72..207 e-value: 2.40113E-8 score: 51.1801
None	No IPR available	SUPERFAMILY	102114	Radical SAM enzymes	coord: 44..272

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0001853.1	Lag0001853.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009102	biotin biosynthetic process
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0004076	biotin synthase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0051536	iron-sulfur cluster binding