Lag0001824 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0001824
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein TIC 22-like, chloroplastic
Locationchr4: 35842824 .. 35846674 (-)
RNA-Seq ExpressionLag0001824
SyntenyLag0001824
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCGCTCGTTTTGATTGCTTCTCTCCCTGCGAAGAAAACGCACCTAAAGCGTTGAGTCCATTTTCTTCTGCAACTGCAAACCCCACACTCTCCCTTCTTCCTCCCGACGAAATGAGTTTCCGAGGAAATGACCCAAATTCTTCTCTTCCTAAACCCAATCAACTCGAACGCAGCCTTCGTCAAGCTCTTGCCAGCTTTCAAAACCAGTGCTCTGGGGTACTCCAGGGCCTTTCCAACCTTCCGCCCTTCAATCCCCCTCTTGCGCTCAAAACCCAACTTCAATCTACCTTCTCGAGCCTCCAAAACCAAGCGAAACGGGCACTTGACTCCAGAATTTCAGCCTTTGATCCCGTAGCTAAAAACCCAGTTTGGGCTCGGGTTGCTGAAACTGCGAAGACCCAATTTAGCCCTTCTGCCAGACTTGGTGTCGCCTTGTCGACGGAAGCCATCGAAGAGCGGTTAGCTGGTATTCCTGTTTATGCCCTTAGCAACGCGGCTGAGGAATTCGTCTTGGTTTCTGGTGCTTCGACTGCAAAATCTCTGGGTTTGTTTTGCTTTAAGAAGGAGGATGCTGAAGCGCTTCTTGAGCACATTGGAACGATGGATGCTTCAGCGCGGAACGGGTCTAAAGTGGTGCCCGTTGCTTTGAATAAAGTGGGTATTACTAATTTCCGCTATGTTTTGTGTGTATAAGAGCTTCTCTTGTCTTTGCAATCCAATATTGTTGTCTGTTCCCGCAGGTTTTTCAACTTCATGTTAATGGGGTGGCCTTCAGGTTGATTCCGGAGTGCTCTCAGGTGAAAAACGCTTTGAGTGTAAGTTCTCTCTCGCTCTCTCTGTCTCTCCCTATGGCGTTAGTTGGAAAGCGGGAATGGTTTTTGAATTTAGTTTCTCTCATATTCTGTTTTCTTTGAAATGTCAAATCACAGTATCTGTTACTAGTTTCTCTTCAGTACAGAAAATCCTTTGCTGTTTTTCTTCATCTGCTTGTAGCCGAGGCCCTAAATTCACTCTATGCACTTGTCATCATCTTTATTTACTGTACATAAACAATATGAATTTGCCTGCGTAGGAAAGAGAAAAGGCTGGCATTTCTGCTGATGGATTTCCCGGGGTTCCTGTTTTCCAGGTTAATTTCTAACATTTCTTAAATCAAAATTGTTGTTCATAGTTCATTTGTTGATCACTATGCAATTCACATTCAATGGTAGAGAAAGTAGTACCTAGGAAACTGCTAACCTTTTACTTGGAAAGTATAGTCCTGATTAATATCGAGTTTGTTTTTTTGAAAATCATGTTCTCGTTGTTGGGGTTTTCACTTTTCAAAAGTACTTTGGAAATCTTCCTATCCAAAAAATTACTTTTGAAGTCTCAGTCAAATTTCAAAAATAAGAAACAGAATTTTGAAATCTAGGCTATGATTTCAAAAAAGTTTTAAAGGTAAATTAGAAAGCAAAAGAAAAGGTTAGCAAATAAGCATAACGCTCAAAAACTGAAAACTAAAACTAAAAACTAAAACTAAGAACATTTTCAAATGGGTCCATATCAACCAAAAAACTTTTGTTTTTCAGGAATGCCGAAATCTATTAATTCTGTTCTTGAAATTAACAGTCATGGGAATGATGGCATGCTTTTTCTGAGTATGAGGTTACTAGTTGCCTTGTTAAAAGTTGTATGATTATATTTTAATTTTTAACATTTAATTTTAATATTTAAACATTTAATTAATGCCATAGATTTTAGTTCTCATCATTTTCTTTCCCAATGATTGAACGCTGAACATTGAGTTGAAGGATCAGTTTGTATATTAACTTTCTAGAGCAACAAACCTGTATACACAAATCCAATCCCTTTTCTTTTGTATTTTTTAAAGATTATAATTGTTTATGATGCAGAACTGTACGACTTTGAGAATTTAGTTTTAAATTTGATTCCGCTAGGGTGGTCAACTGTTTGGGATATTGATAGGAGTTTGATATTTTGAAACTCTAGGATGGTTTCTGAGATTTGATCAGAGCAGTTATCGTGTAAAAAAGAATGGAAGCAAACAGAATAAAACCAAATGAACAAAAAATATGAATTTATTTGTGTACCTCAATAATAAGAAGAATGTTGCAGTTCAAAAAAGAAGCAAGGAAAGGTGGAATCAGTGTATCTATCCCTGTTTTTTCCTCCTGCTTTCTTTCAACAATTGAAACCTACACAATTTAGAAGCAGTGTTATTGAGGCAAGTGCCTCAATGTGCCTATGGCTAGTGGTGAGGTGCCTCTTGCCTTCAATGCCTCAGCTCAAGTCCATGGCATGCACACATTATTGCCACCATGAGCATAAAGTTCATGGTGAGCCATAGTGAATTAATCGAGACAAACTCAAACTTGAAGGAAAAAAACTAGTATTTTCTAGGGAGAGAAGAAAATCCACACAACAACTTTCTTCAGCTTGCCCTCCTGGCCTTTGAAGCTTCAACTCCAACCTCATTCCTTTTAACAGCTCAAAATAGGAAGGAAAAGAGAACAAAAAGAAAATCTTTTCTCCTCTTCGATTACCCTCTAAGTTGCAAGGAATCCATGATAGTCTATGCATCCTTGCATCGGTTCAAGTTTTCAAATATACAAACTCTTTAAGACTTCTATTATTGTTTCTGATTTTCTATAAAAATTTCAATTTTCTTTGTTTCAATTCGTCAATCTTTTTTTTTAAAAAAAAAAATTATAGGAACTATGGATATTGTTTGTTTTTTTTATTCTTTATTTATGTTTTCAGTTTCATATATTAACTATTGTTCTCAAGCTTGAAGGCTATGGCATTCTATTTTAAAGAATTTATAGTGTATAATTTATTAGTTTACTTAAATACACCTTGTTTCACTATGTTTCTTGGTCTCTTCTTGTTTCTATGCCTTTTGGTTGCTTCATGCCTCAAGGTGATCTCGAGACCTGTCGCCTTAGAACATAGGCTAAGCCCTCCGGGGTCAGCCAAGGCATCATGGATTTAGAACTCTGCTGAGCTGCCTATTTGGGGAATCAAATATACCTTTGATTTTTTAAACTACAAATATAGAAATGAATAGGGTTAAATGATATTCCCGATTTCAAATGTAATGAAGCATAGTTATATGAAGTTCCATTCTCCTCAGCTGTCGCATACACGGGTTTCTTTTAGTTTTCAATCCAGTTAATATTACCTTGTTTAAGGATTTCTAAACATTTGGGACTGATGTAATCAAGTTTTTGGACTTTTCATAGTCAAAGAGTTTGATACTGAGGAGCCAAAACAAGAGCTACCGTCCTGCCTTTTTCAGAAAGGTTAGTTATGATATGAAGAGTAGTTTTCTTGACATGGACTATCATCTTTACATGGATTCATTGTTATAACTCTCATTCTTGGATTTTTGTAGGAGGATTTGGAGAATTCTCTACAAAGAGCATCTCGTGAACAAAATCAAATCAATCCTGCCTTCCGACCTGGGGATATACAGGTATAGCTAGCAGCTAGCTCTTCGCGCTTGTTGTTCGTGAAGTATGTTCTGTGGATGGGAACTTTAAAGTTCTGTCTGAATCTTGTAGGTTGCTGTTTTTGAAGAAATTTTAAAAGGAATGAAGGTGAGAAAGAAGCCTATCTCTTTTTTATTTTCTAGGAACTCTCTCTGTTTCTCTCTTATCTAAGAAAGAACTCTTTTTAACTCTCTTTAAATGTTTGTTAAGAGCCAACAATAATCGTAAACCTTTTAATCATTCTAAAGTTGTAGCTTTGTTCATGGACAGGATAATTCTATTTCAACATGGGATGATGTAGTCTTCATTCCTCCTGGATTTGATGTTTCAACTGACCCCAAAAAACAACATTAG

mRNA sequence

ATGCTCGCTCGTTTTGATTGCTTCTCTCCCTGCGAAGAAAACGCACCTAAAGCGTTGAGTCCATTTTCTTCTGCAACTGCAAACCCCACACTCTCCCTTCTTCCTCCCGACGAAATGAGTTTCCGAGGAAATGACCCAAATTCTTCTCTTCCTAAACCCAATCAACTCGAACGCAGCCTTCGTCAAGCTCTTGCCAGCTTTCAAAACCAGTGCTCTGGGGTACTCCAGGGCCTTTCCAACCTTCCGCCCTTCAATCCCCCTCTTGCGCTCAAAACCCAACTTCAATCTACCTTCTCGAGCCTCCAAAACCAAGCGAAACGGGCACTTGACTCCAGAATTTCAGCCTTTGATCCCGTAGCTAAAAACCCAGTTTGGGCTCGGGTTGCTGAAACTGCGAAGACCCAATTTAGCCCTTCTGCCAGACTTGGTGTCGCCTTGTCGACGGAAGCCATCGAAGAGCGGTTAGCTGGTATTCCTGTTTATGCCCTTAGCAACGCGGCTGAGGAATTCGTCTTGGTTTCTGGTGCTTCGACTGCAAAATCTCTGGGTTTGTTTTGCTTTAAGAAGGAGGATGCTGAAGCGCTTCTTGAGCACATTGGAACGATGGATGCTTCAGCGCGGAACGGGTCTAAAGTGGTGCCCGTTGCTTTGAATAAAGTTTTTCAACTTCATGTTAATGGGGTGGCCTTCAGGTTGATTCCGGAGTGCTCTCAGGTGAAAAACGCTTTGAGTGAAAGAGAAAAGGCTGGCATTTCTGCTGATGGATTTCCCGGGGTTCCTGTTTTCCAGTCAAAGAGTTTGATACTGAGGAGCCAAAACAAGAGCTACCGTCCTGCCTTTTTCAGAAAGGAGGATTTGGAGAATTCTCTACAAAGAGCATCTCGTGAACAAAATCAAATCAATCCTGCCTTCCGACCTGGGGATATACAGGTTGCTGTTTTTGAAGAAATTTTAAAAGGAATGAAGGATAATTCTATTTCAACATGGGATGATGTAGTCTTCATTCCTCCTGGATTTGATGTTTCAACTGACCCCAAAAAACAACATTAG

Coding sequence (CDS)

ATGCTCGCTCGTTTTGATTGCTTCTCTCCCTGCGAAGAAAACGCACCTAAAGCGTTGAGTCCATTTTCTTCTGCAACTGCAAACCCCACACTCTCCCTTCTTCCTCCCGACGAAATGAGTTTCCGAGGAAATGACCCAAATTCTTCTCTTCCTAAACCCAATCAACTCGAACGCAGCCTTCGTCAAGCTCTTGCCAGCTTTCAAAACCAGTGCTCTGGGGTACTCCAGGGCCTTTCCAACCTTCCGCCCTTCAATCCCCCTCTTGCGCTCAAAACCCAACTTCAATCTACCTTCTCGAGCCTCCAAAACCAAGCGAAACGGGCACTTGACTCCAGAATTTCAGCCTTTGATCCCGTAGCTAAAAACCCAGTTTGGGCTCGGGTTGCTGAAACTGCGAAGACCCAATTTAGCCCTTCTGCCAGACTTGGTGTCGCCTTGTCGACGGAAGCCATCGAAGAGCGGTTAGCTGGTATTCCTGTTTATGCCCTTAGCAACGCGGCTGAGGAATTCGTCTTGGTTTCTGGTGCTTCGACTGCAAAATCTCTGGGTTTGTTTTGCTTTAAGAAGGAGGATGCTGAAGCGCTTCTTGAGCACATTGGAACGATGGATGCTTCAGCGCGGAACGGGTCTAAAGTGGTGCCCGTTGCTTTGAATAAAGTTTTTCAACTTCATGTTAATGGGGTGGCCTTCAGGTTGATTCCGGAGTGCTCTCAGGTGAAAAACGCTTTGAGTGAAAGAGAAAAGGCTGGCATTTCTGCTGATGGATTTCCCGGGGTTCCTGTTTTCCAGTCAAAGAGTTTGATACTGAGGAGCCAAAACAAGAGCTACCGTCCTGCCTTTTTCAGAAAGGAGGATTTGGAGAATTCTCTACAAAGAGCATCTCGTGAACAAAATCAAATCAATCCTGCCTTCCGACCTGGGGATATACAGGTTGCTGTTTTTGAAGAAATTTTAAAAGGAATGAAGGATAATTCTATTTCAACATGGGATGATGTAGTCTTCATTCCTCCTGGATTTGATGTTTCAACTGACCCCAAAAAACAACATTAG

Protein sequence

MLARFDCFSPCEENAPKALSPFSSATANPTLSLLPPDEMSFRGNDPNSSLPKPNQLERSLRQALASFQNQCSGVLQGLSNLPPFNPPLALKTQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTEAIEERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNGSKVVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLILRSQNKSYRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTWDDVVFIPPGFDVSTDPKKQH
Homology
BLAST of Lag0001824 vs. NCBI nr
Match: XP_022959990.1 (protein TIC 22-like, chloroplastic [Cucurbita moschata])

HSP 1 Score: 553.9 bits (1426), Expect = 9.8e-154
Identity = 284/320 (88.75%), Postives = 301/320 (94.06%), Query Frame = 0

Query: 30  TLSLLPPDEMSFRGNDPNSSLPKPNQLERSLRQALASFQNQCSGVLQGLSNLPPFNPPLA 89
           TLSLLPPDEMS +GNDPNSSLPKP+QLE +LRQALA FQNQCSGVL  LSNLPPFNPPL+
Sbjct: 39  TLSLLPPDEMSSQGNDPNSSLPKPSQLECNLRQALAGFQNQCSGVLHNLSNLPPFNPPLS 98

Query: 90  LKTQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTE 149
           LK+QLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWAR+AETAKT   P AR  VALSTE
Sbjct: 99  LKSQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARIAETAKTP-RPYARPTVALSTE 158

Query: 150 AIEERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNG 209
           AIEERLAGIPVYALSNA+EEFVLVSGASTAKSLGLFCF KEDAE LL HIGT+D SA+NG
Sbjct: 159 AIEERLAGIPVYALSNASEEFVLVSGASTAKSLGLFCFNKEDAETLLGHIGTVDPSAQNG 218

Query: 210 SKVVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLIL 269
           SKVV VALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGIS +GFPGVPVFQSKSLIL
Sbjct: 219 SKVVTVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISDNGFPGVPVFQSKSLIL 278

Query: 270 RSQNKSYRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTW 329
           RSQNKSYRPAFFRKEDLENSL+RAS++QNQ+NPA RPGD+QVA FE+I+KGMKDNSISTW
Sbjct: 279 RSQNKSYRPAFFRKEDLENSLKRASQDQNQMNPALRPGDVQVAAFEDIIKGMKDNSISTW 338

Query: 330 DDVVFIPPGFDVSTDPKKQH 350
           DDVVFIPPGFDVSTDPKKQH
Sbjct: 339 DDVVFIPPGFDVSTDPKKQH 357

BLAST of Lag0001824 vs. NCBI nr
Match: XP_023005035.1 (protein TIC 22-like, chloroplastic [Cucurbita maxima])

HSP 1 Score: 550.8 bits (1418), Expect = 8.3e-153
Identity = 282/320 (88.12%), Postives = 300/320 (93.75%), Query Frame = 0

Query: 30  TLSLLPPDEMSFRGNDPNSSLPKPNQLERSLRQALASFQNQCSGVLQGLSNLPPFNPPLA 89
           TLSLLPPDEMS +GNDPNSSLPKP+QLE +LRQALA FQNQCSGVL  LSNLPPFNPPL+
Sbjct: 40  TLSLLPPDEMSSQGNDPNSSLPKPSQLECNLRQALAGFQNQCSGVLHNLSNLPPFNPPLS 99

Query: 90  LKTQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTE 149
           LK+QLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWAR+AETAKT   P AR  VALSTE
Sbjct: 100 LKSQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARIAETAKTP-RPYARPTVALSTE 159

Query: 150 AIEERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNG 209
           AIEERLAGIPVYALSNA+EEFVLVSGASTAKSLG FCF KEDAE LL HIGT+D SA+NG
Sbjct: 160 AIEERLAGIPVYALSNASEEFVLVSGASTAKSLGFFCFNKEDAETLLGHIGTVDPSAQNG 219

Query: 210 SKVVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLIL 269
           SKVV VALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGIS +GFPGVPVFQSKSLIL
Sbjct: 220 SKVVTVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISDNGFPGVPVFQSKSLIL 279

Query: 270 RSQNKSYRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTW 329
           RS+NKSYRPAFFRKEDLENSL+RAS++QNQ+NPA RPGD+QVA FE+I+KGMKDNSISTW
Sbjct: 280 RSKNKSYRPAFFRKEDLENSLKRASQDQNQMNPALRPGDVQVAAFEDIIKGMKDNSISTW 339

Query: 330 DDVVFIPPGFDVSTDPKKQH 350
           DDVVFIPPGFDVSTDPKKQH
Sbjct: 340 DDVVFIPPGFDVSTDPKKQH 358

BLAST of Lag0001824 vs. NCBI nr
Match: XP_023514114.1 (protein TIC 22-like, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548.1 bits (1411), Expect = 5.4e-152
Identity = 281/320 (87.81%), Postives = 298/320 (93.12%), Query Frame = 0

Query: 30  TLSLLPPDEMSFRGNDPNSSLPKPNQLERSLRQALASFQNQCSGVLQGLSNLPPFNPPLA 89
           TLSLLPPDEMS +GNDPNSSLPKP+QLE +LRQALA  QN CSGVL  LSNLPPFNPPL+
Sbjct: 84  TLSLLPPDEMSSQGNDPNSSLPKPSQLECNLRQALAGIQNHCSGVLHNLSNLPPFNPPLS 143

Query: 90  LKTQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTE 149
           LK+QLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWAR+AETAKT   P AR  VALSTE
Sbjct: 144 LKSQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARIAETAKTP-RPYARPTVALSTE 203

Query: 150 AIEERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNG 209
           AIEERLAGIPVYALSNA+EEFVLVSGASTAKSLG FCF KEDAE LL HIGT+D SA+NG
Sbjct: 204 AIEERLAGIPVYALSNASEEFVLVSGASTAKSLGFFCFNKEDAETLLGHIGTVDPSAQNG 263

Query: 210 SKVVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLIL 269
           SKVV VALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGIS +GFPGVPVFQSKSLIL
Sbjct: 264 SKVVTVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISDNGFPGVPVFQSKSLIL 323

Query: 270 RSQNKSYRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTW 329
           RSQNKSYRPAFFRKEDLENSL+RAS++QNQ+NPA RPGD+QVA FE+I+KGMKDNSISTW
Sbjct: 324 RSQNKSYRPAFFRKEDLENSLKRASQDQNQMNPALRPGDVQVAAFEDIIKGMKDNSISTW 383

Query: 330 DDVVFIPPGFDVSTDPKKQH 350
           DDVVFIPPGFDVSTDPKKQH
Sbjct: 384 DDVVFIPPGFDVSTDPKKQH 402

BLAST of Lag0001824 vs. NCBI nr
Match: KAG7025300.1 (Protein TIC 22-like, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 535.4 bits (1378), Expect = 3.6e-148
Identity = 274/311 (88.10%), Postives = 291/311 (93.57%), Query Frame = 0

Query: 39  MSFRGNDPNSSLPKPNQLERSLRQALASFQNQCSGVLQGLSNLPPFNPPLALKTQLQSTF 98
           MS +GNDPNSSLPKP+QLE +LRQALA FQNQCSGVL  LSNLPPFNPPL+LK+QLQSTF
Sbjct: 1   MSSQGNDPNSSLPKPSQLECNLRQALAGFQNQCSGVLHNLSNLPPFNPPLSLKSQLQSTF 60

Query: 99  SSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTEAIEERLAGI 158
           SSLQNQAKRALDSRISAFDPVAKNPVWAR+AETAKT   P AR  VALSTEAIEERLAGI
Sbjct: 61  SSLQNQAKRALDSRISAFDPVAKNPVWARIAETAKTP-RPYARPTVALSTEAIEERLAGI 120

Query: 159 PVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNGSKVVPVALN 218
           PVYALSNA+EEFVLVSGASTAKSLG FCF KEDAE LL HIGT+D SA+NGSKVV VALN
Sbjct: 121 PVYALSNASEEFVLVSGASTAKSLGFFCFNKEDAETLLGHIGTVDPSAQNGSKVVTVALN 180

Query: 219 KVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLILRSQNKSYRP 278
           KVFQLHVNGVAFRLIPECSQVKNALSEREKAGIS +GFPGVPVFQSKSLILRSQNKSYRP
Sbjct: 181 KVFQLHVNGVAFRLIPECSQVKNALSEREKAGISDNGFPGVPVFQSKSLILRSQNKSYRP 240

Query: 279 AFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTWDDVVFIPPG 338
           AFFRKEDLENSL+RAS++QNQ+NPA RPGD+QVA FE+I+KGMKDNSISTWDDVVFIPPG
Sbjct: 241 AFFRKEDLENSLKRASQDQNQMNPALRPGDVQVAAFEDIIKGMKDNSISTWDDVVFIPPG 300

Query: 339 FDVSTDPKKQH 350
           FDVSTDPKKQH
Sbjct: 301 FDVSTDPKKQH 310

BLAST of Lag0001824 vs. NCBI nr
Match: KAG6592897.1 (Protein TIC 22-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 531.9 bits (1369), Expect = 4.0e-147
Identity = 273/311 (87.78%), Postives = 288/311 (92.60%), Query Frame = 0

Query: 39  MSFRGNDPNSSLPKPNQLERSLRQALASFQNQCSGVLQGLSNLPPFNPPLALKTQLQSTF 98
           MS +GNDPNSSLPKP+Q E +LRQALA FQNQCSGVL  LSNLPPFNPPL LK+QLQSTF
Sbjct: 1   MSSQGNDPNSSLPKPSQFECNLRQALAGFQNQCSGVLHNLSNLPPFNPPLFLKSQLQSTF 60

Query: 99  SSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTEAIEERLAGI 158
           SSLQNQAKRALDSRISAFDPVAKNPVWAR+AETAKT   P AR  VALSTEAIEERLAGI
Sbjct: 61  SSLQNQAKRALDSRISAFDPVAKNPVWARIAETAKTP-RPYARPTVALSTEAIEERLAGI 120

Query: 159 PVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNGSKVVPVALN 218
           PVYALSNA+EEFVLVSGASTAKSLG FCF KEDAE LL HIGT+D SA+NGSKVV VALN
Sbjct: 121 PVYALSNASEEFVLVSGASTAKSLGFFCFNKEDAETLLGHIGTVDPSAQNGSKVVTVALN 180

Query: 219 KVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLILRSQNKSYRP 278
           KVFQLHVNGVAFRLIPECSQVKNALSEREKAGIS  GFPGVPVFQSKSLILRSQNKSYRP
Sbjct: 181 KVFQLHVNGVAFRLIPECSQVKNALSEREKAGISDHGFPGVPVFQSKSLILRSQNKSYRP 240

Query: 279 AFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTWDDVVFIPPG 338
           AFFRKEDLENSL+RAS++QNQ+NPA RPGD+QVA FE+I+KGMKDNSISTWDDVVFIPPG
Sbjct: 241 AFFRKEDLENSLKRASQDQNQMNPALRPGDVQVAAFEDIIKGMKDNSISTWDDVVFIPPG 300

Query: 339 FDVSTDPKKQH 350
           FDVSTDPKKQH
Sbjct: 301 FDVSTDPKKQH 310

BLAST of Lag0001824 vs. ExPASy Swiss-Prot
Match: F4J469 (Protein TIC 22-like, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC22L PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-85
Identity = 180/313 (57.51%), Postives = 231/313 (73.80%), Query Frame = 0

Query: 47  NSSLPKPNQLERSL---RQALASFQNQCSGVLQGLSNL--PPFNPPL-ALKTQLQSTFSS 106
           NS++  P++ +  L   +Q+ ++ Q+QCS +L  +S    P FN      K  + S  +S
Sbjct: 2   NSNIFPPSKQQNELNNIQQSFSNLQSQCSNLLLNVSQTLNPLFNANTNNNKPNIFSALNS 61

Query: 107 LQNQAKRALDSRISAFDPVAKNPVWARVAET---AKTQFS-PSARLGVALSTEAIEERLA 166
            ++QAK+ALDSRIS F+   K PVWAR+++    A+ Q + P    G  LS +AIEERLA
Sbjct: 62  FRDQAKQALDSRISRFNS-GKAPVWARISDDGGGARAQVTVPIRGSGKGLSADAIEERLA 121

Query: 167 GIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASAR-NGSKVVPV 226
           G+PVYALSN+ EEFVLVSG S+ KSLGL   K+EDAE LL+ + +MD   R  GSKVV +
Sbjct: 122 GVPVYALSNSNEEFVLVSGTSSGKSLGLLFCKEEDAETLLKEMKSMDPRMRKEGSKVVAL 181

Query: 227 ALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLILRSQNKS 286
           AL+KVFQL VNGVAFRLIPE +QVKNAL ER+ AGI  D F GVPVFQSKSLILRS+N S
Sbjct: 182 ALSKVFQLKVNGVAFRLIPESTQVKNALKERKTAGIDDDDFHGVPVFQSKSLILRSENMS 241

Query: 287 YRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTWDDVVFI 346
           YRP FFRKEDLE SL RAS +QN++NPA +PGDIQVAVFE+I+KGM++++ S WDD+VFI
Sbjct: 242 YRPVFFRKEDLEKSLIRASSQQNRLNPALKPGDIQVAVFEDIVKGMRESTTSNWDDIVFI 301

Query: 347 PPGFDVSTDPKKQ 349
           PPGF+VST+  ++
Sbjct: 302 PPGFEVSTEQTQE 313

BLAST of Lag0001824 vs. ExPASy Swiss-Prot
Match: Q9ZST9 (Protein TIC 22, chloroplastic OS=Pisum sativum OX=3888 GN=TIC22 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 5.4e-38
Identity = 86/194 (44.33%), Postives = 120/194 (61.86%), Query Frame = 0

Query: 146 LSTEAIEERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDAS 205
           L+   + + LAG  VY +SN+  EFVL+S A  AKS+GL CF++EDAEA L  + +    
Sbjct: 53  LTPNHVAKSLAGTSVYTVSNSDNEFVLMSDAEGAKSIGLLCFRQEDAEAFLAQVRSRKKE 112

Query: 206 ARNGSKVVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSK 265
            R G+KVVP+ L++V+ L V G+AFR +P+  Q+KNAL   E    +   F GVPVFQS 
Sbjct: 113 FRGGAKVVPITLDQVYMLKVEGIAFRFLPDPVQIKNAL---ELRAANRGSFDGVPVFQSD 172

Query: 266 SLILRSQNKSYRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMK-DN 325
            L+++ +NK Y P +F KEDLE  L + SR    +  +     I V  FE++LK M+   
Sbjct: 173 LLVVKKKNKRYCPVYFSKEDLEYELSKVSRSSKGVGVS---QHIMVGSFEDVLKKMELSE 232

Query: 326 SISTWDDVVFIPPG 339
             S W+D+VFIPPG
Sbjct: 233 KSSGWEDLVFIPPG 240

BLAST of Lag0001824 vs. ExPASy Swiss-Prot
Match: Q9SZB2 (Protein TIC 22, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC22 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.1e-34
Identity = 93/251 (37.05%), Postives = 139/251 (55.38%), Query Frame = 0

Query: 92  TQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTEAI 151
           T+  S  S+     KR  ++  +    +   P +A V++      S S      LS   +
Sbjct: 22  TRFSSDLSARIEDTKRFAETLATRRFSLPTPPPFASVSQ------SKSGTPTTTLSPSLV 81

Query: 152 EERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNGSK 211
            + LAG  V+ +SN   EFVL+S  +  KS+GL CF++EDAEA L          +  +K
Sbjct: 82  AKALAGTSVFTVSNTNNEFVLISDPTGGKSIGLLCFRQEDAEAFLAQARLRRRELKTNAK 141

Query: 212 VVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLILRS 271
           VVP+ L++V+ L V G++FR +P+  Q+KNAL E + +G + +GF GVPVFQS+ L++R 
Sbjct: 142 VVPITLDQVYLLKVEGISFRFLPDPIQIKNAL-ELKSSG-NKNGFDGVPVFQSELLVVRK 201

Query: 272 QNKSYRPAFFRKEDLENSLQ---RASREQNQINPAFRPGDIQVAVFEEILKGMK-DNSIS 331
           +N+ Y P +F KED+E  L    RASR   Q         I V   E++L+ M+     S
Sbjct: 202 KNRRYCPVYFSKEDIERELSKYTRASRGDQQ---------IMVGSLEDVLRKMEMSEKNS 255

Query: 332 TWDDVVFIPPG 339
            W+DV+FIPPG
Sbjct: 262 GWEDVIFIPPG 255

BLAST of Lag0001824 vs. ExPASy TrEMBL
Match: A0A6J1H7U6 (protein TIC 22-like, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460879 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.8e-154
Identity = 284/320 (88.75%), Postives = 301/320 (94.06%), Query Frame = 0

Query: 30  TLSLLPPDEMSFRGNDPNSSLPKPNQLERSLRQALASFQNQCSGVLQGLSNLPPFNPPLA 89
           TLSLLPPDEMS +GNDPNSSLPKP+QLE +LRQALA FQNQCSGVL  LSNLPPFNPPL+
Sbjct: 39  TLSLLPPDEMSSQGNDPNSSLPKPSQLECNLRQALAGFQNQCSGVLHNLSNLPPFNPPLS 98

Query: 90  LKTQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTE 149
           LK+QLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWAR+AETAKT   P AR  VALSTE
Sbjct: 99  LKSQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARIAETAKTP-RPYARPTVALSTE 158

Query: 150 AIEERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNG 209
           AIEERLAGIPVYALSNA+EEFVLVSGASTAKSLGLFCF KEDAE LL HIGT+D SA+NG
Sbjct: 159 AIEERLAGIPVYALSNASEEFVLVSGASTAKSLGLFCFNKEDAETLLGHIGTVDPSAQNG 218

Query: 210 SKVVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLIL 269
           SKVV VALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGIS +GFPGVPVFQSKSLIL
Sbjct: 219 SKVVTVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISDNGFPGVPVFQSKSLIL 278

Query: 270 RSQNKSYRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTW 329
           RSQNKSYRPAFFRKEDLENSL+RAS++QNQ+NPA RPGD+QVA FE+I+KGMKDNSISTW
Sbjct: 279 RSQNKSYRPAFFRKEDLENSLKRASQDQNQMNPALRPGDVQVAAFEDIIKGMKDNSISTW 338

Query: 330 DDVVFIPPGFDVSTDPKKQH 350
           DDVVFIPPGFDVSTDPKKQH
Sbjct: 339 DDVVFIPPGFDVSTDPKKQH 357

BLAST of Lag0001824 vs. ExPASy TrEMBL
Match: A0A6J1KWA0 (protein TIC 22-like, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111498148 PE=4 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 4.0e-153
Identity = 282/320 (88.12%), Postives = 300/320 (93.75%), Query Frame = 0

Query: 30  TLSLLPPDEMSFRGNDPNSSLPKPNQLERSLRQALASFQNQCSGVLQGLSNLPPFNPPLA 89
           TLSLLPPDEMS +GNDPNSSLPKP+QLE +LRQALA FQNQCSGVL  LSNLPPFNPPL+
Sbjct: 40  TLSLLPPDEMSSQGNDPNSSLPKPSQLECNLRQALAGFQNQCSGVLHNLSNLPPFNPPLS 99

Query: 90  LKTQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTE 149
           LK+QLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWAR+AETAKT   P AR  VALSTE
Sbjct: 100 LKSQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARIAETAKTP-RPYARPTVALSTE 159

Query: 150 AIEERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNG 209
           AIEERLAGIPVYALSNA+EEFVLVSGASTAKSLG FCF KEDAE LL HIGT+D SA+NG
Sbjct: 160 AIEERLAGIPVYALSNASEEFVLVSGASTAKSLGFFCFNKEDAETLLGHIGTVDPSAQNG 219

Query: 210 SKVVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLIL 269
           SKVV VALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGIS +GFPGVPVFQSKSLIL
Sbjct: 220 SKVVTVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISDNGFPGVPVFQSKSLIL 279

Query: 270 RSQNKSYRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTW 329
           RS+NKSYRPAFFRKEDLENSL+RAS++QNQ+NPA RPGD+QVA FE+I+KGMKDNSISTW
Sbjct: 280 RSKNKSYRPAFFRKEDLENSLKRASQDQNQMNPALRPGDVQVAAFEDIIKGMKDNSISTW 339

Query: 330 DDVVFIPPGFDVSTDPKKQH 350
           DDVVFIPPGFDVSTDPKKQH
Sbjct: 340 DDVVFIPPGFDVSTDPKKQH 358

BLAST of Lag0001824 vs. ExPASy TrEMBL
Match: A0A6J1D9F7 (protein TIC 22-like, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018590 PE=4 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 4.8e-138
Identity = 271/352 (76.99%), Postives = 286/352 (81.25%), Query Frame = 0

Query: 1   MLARFDCFSPCEENAPKAL--SPFSSATANPTLSLLPPDEMSFRGNDPNSSLPKPNQLER 60
           MLA+ D  S    N       SPF            P DEMSF GNDPNSS       ER
Sbjct: 1   MLAQSDSLSHLRRNRSSIFCNSPF-----------FPLDEMSFHGNDPNSS------FER 60

Query: 61  SLRQALASFQNQCSGVLQGLSNLPPFNPPLALKTQLQSTFSSLQNQAKRALDSRISAFDP 120
           +LRQAL S QN CSG+L  LS+LPPFNPPL  KT        LQNQ KRALDS IS  DP
Sbjct: 61  NLRQALYSLQNHCSGLLHTLSHLPPFNPPLPFKTH-------LQNQPKRALDSAISPSDP 120

Query: 121 VAKNPVWARVAETAKTQFSPSAR--LGVALSTEAIEERLAGIPVYALSNAAEEFVLVSGA 180
            AKNP+WARVAE+A TQFSPSAR     ALSTEAIEERLAGIPVYALSN+AEEFVLVSGA
Sbjct: 121 AAKNPLWARVAESATTQFSPSARPAAAAALSTEAIEERLAGIPVYALSNSAEEFVLVSGA 180

Query: 181 STAKSLGLFCFKKEDAEALLEHIGTMDASARNGSKVVPVALNKVFQLHVNGVAFRLIPEC 240
           STAKSLGLFCFKKEDAEALL+H+G MDASA+NGSKVVPVALNKVFQLHVNGV FRL+PEC
Sbjct: 181 STAKSLGLFCFKKEDAEALLQHVGMMDASAQNGSKVVPVALNKVFQLHVNGVTFRLVPEC 240

Query: 241 SQVKNALSEREKAGISADGFPGVPVFQSKSLILRSQNKSYRPAFFRKEDLENSLQRASRE 300
           SQVKNALSER+KAGISADGF GVPVFQSKSLILRSQNKSYRPAFFRKEDLE SLQRASRE
Sbjct: 241 SQVKNALSERKKAGISADGFSGVPVFQSKSLILRSQNKSYRPAFFRKEDLEKSLQRASRE 300

Query: 301 QNQINPAFRPGDIQVAVFEEILKGMKDNSISTWDDVVFIPPGFDVSTDPKKQ 349
           QNQ+NPAFRPGDIQVA FEE++KGMKDNSISTWDDVVFIPPGFDVSTDPKKQ
Sbjct: 301 QNQVNPAFRPGDIQVAGFEEVIKGMKDNSISTWDDVVFIPPGFDVSTDPKKQ 328

BLAST of Lag0001824 vs. ExPASy TrEMBL
Match: A0A6J1DA19 (protein TIC 22-like, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018590 PE=4 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 2.3e-124
Identity = 254/352 (72.16%), Postives = 268/352 (76.14%), Query Frame = 0

Query: 1   MLARFDCFSPCEENAPKAL--SPFSSATANPTLSLLPPDEMSFRGNDPNSSLPKPNQLER 60
           MLA+ D  S    N       SPF            P DEMSF GNDPNSS       ER
Sbjct: 1   MLAQSDSLSHLRRNRSSIFCNSPF-----------FPLDEMSFHGNDPNSS------FER 60

Query: 61  SLRQALASFQNQCSGVLQGLSNLPPFNPPLALKTQLQSTFSSLQNQAKRALDSRISAFDP 120
           +LRQAL S QN CSG+L  LS+LPPFNPPL  KT        LQNQ KRALDS IS  DP
Sbjct: 61  NLRQALYSLQNHCSGLLHTLSHLPPFNPPLPFKTH-------LQNQPKRALDSAISPSDP 120

Query: 121 VAKNPVWARVAETAKTQFSPSAR--LGVALSTEAIEERLAGIPVYALSNAAEEFVLVSGA 180
            AKNP+WARVAE+A TQFSPSAR     ALSTEAIEERLAGIPVYALSN+AEEFVLVSGA
Sbjct: 121 AAKNPLWARVAESATTQFSPSARPAAAAALSTEAIEERLAGIPVYALSNSAEEFVLVSGA 180

Query: 181 STAKSLGLFCFKKEDAEALLEHIGTMDASARNGSKVVPVALNKVFQLHVNGVAFRLIPEC 240
           STAKSLGLFCFKKEDAEALL+H+G MDASA+NGSKVVPVALNKVFQLHVNGV FRL+PEC
Sbjct: 181 STAKSLGLFCFKKEDAEALLQHVGMMDASAQNGSKVVPVALNKVFQLHVNGVTFRLVPEC 240

Query: 241 SQVKNALSEREKAGISADGFPGVPVFQSKSLILRSQNKSYRPAFFRKEDLENSLQRASRE 300
           SQVKNALS                   SKSLILRSQNKSYRPAFFRKEDLE SLQRASRE
Sbjct: 241 SQVKNALS-------------------SKSLILRSQNKSYRPAFFRKEDLEKSLQRASRE 300

Query: 301 QNQINPAFRPGDIQVAVFEEILKGMKDNSISTWDDVVFIPPGFDVSTDPKKQ 349
           QNQ+NPAFRPGDIQVA FEE++KGMKDNSISTWDDVVFIPPGFDVSTDPKKQ
Sbjct: 301 QNQVNPAFRPGDIQVAGFEEVIKGMKDNSISTWDDVVFIPPGFDVSTDPKKQ 309

BLAST of Lag0001824 vs. ExPASy TrEMBL
Match: A0A5D3BU87 (Protein TIC 22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold128G00260 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 1.0e-119
Identity = 242/313 (77.32%), Postives = 259/313 (82.75%), Query Frame = 0

Query: 39  MSFRGNDPNSSLPKPNQLERSLRQALASFQNQCSGVLQGLSNLPPFNPPLALKTQLQSTF 98
           MS  GNDP SS         +L QAL SFQN CS +L  LSN      PL LKTQLQSTF
Sbjct: 1   MSSHGNDPISSF-------LNLPQALNSFQNHCSTLLHNLSN------PLPLKTQLQSTF 60

Query: 99  SSLQN-QAKRALDSRISAFDPVA-KNPVWARVAETAKTQFS-PSARLGVALSTEAIEERL 158
           S+L N +    L S IS  D V  KNP+WAR+ ETAKTQFS PS+   ++LSTEAIEERL
Sbjct: 61  STLLNPKPNPPLHSTISPSDSVPNKNPLWARLPETAKTQFSRPSSPSPLSLSTEAIEERL 120

Query: 159 AGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNGSKVVPV 218
           AGIPVYALSNA+EEFVLVSGAST KSLG FCFKK+DAE LL+HIGTMD SA  GSKVVPV
Sbjct: 121 AGIPVYALSNASEEFVLVSGASTQKSLGFFCFKKDDAETLLQHIGTMDPSAHYGSKVVPV 180

Query: 219 ALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLILRSQNKS 278
           ALNKVFQLHVNGVAFRLIPECSQVKNAL+ER+KAGISAD F GVPVFQSKSL+LRSQNKS
Sbjct: 181 ALNKVFQLHVNGVAFRLIPECSQVKNALTERKKAGISADEFSGVPVFQSKSLVLRSQNKS 240

Query: 279 YRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTWDDVVFI 338
           YRPAFFRKEDLENSLQ A REQNQINPA RPGDIQVAVFEEI+KGMKDNSISTWDD+VFI
Sbjct: 241 YRPAFFRKEDLENSLQSACREQNQINPALRPGDIQVAVFEEIIKGMKDNSISTWDDIVFI 300

Query: 339 PPGFDVSTDPKKQ 349
           PPGFDVSTDPKKQ
Sbjct: 301 PPGFDVSTDPKKQ 300

BLAST of Lag0001824 vs. TAIR 10
Match: AT3G23710.1 (Tic22-like family protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.4e-86
Identity = 180/313 (57.51%), Postives = 231/313 (73.80%), Query Frame = 0

Query: 47  NSSLPKPNQLERSL---RQALASFQNQCSGVLQGLSNL--PPFNPPL-ALKTQLQSTFSS 106
           NS++  P++ +  L   +Q+ ++ Q+QCS +L  +S    P FN      K  + S  +S
Sbjct: 2   NSNIFPPSKQQNELNNIQQSFSNLQSQCSNLLLNVSQTLNPLFNANTNNNKPNIFSALNS 61

Query: 107 LQNQAKRALDSRISAFDPVAKNPVWARVAET---AKTQFS-PSARLGVALSTEAIEERLA 166
            ++QAK+ALDSRIS F+   K PVWAR+++    A+ Q + P    G  LS +AIEERLA
Sbjct: 62  FRDQAKQALDSRISRFNS-GKAPVWARISDDGGGARAQVTVPIRGSGKGLSADAIEERLA 121

Query: 167 GIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASAR-NGSKVVPV 226
           G+PVYALSN+ EEFVLVSG S+ KSLGL   K+EDAE LL+ + +MD   R  GSKVV +
Sbjct: 122 GVPVYALSNSNEEFVLVSGTSSGKSLGLLFCKEEDAETLLKEMKSMDPRMRKEGSKVVAL 181

Query: 227 ALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLILRSQNKS 286
           AL+KVFQL VNGVAFRLIPE +QVKNAL ER+ AGI  D F GVPVFQSKSLILRS+N S
Sbjct: 182 ALSKVFQLKVNGVAFRLIPESTQVKNALKERKTAGIDDDDFHGVPVFQSKSLILRSENMS 241

Query: 287 YRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMKDNSISTWDDVVFI 346
           YRP FFRKEDLE SL RAS +QN++NPA +PGDIQVAVFE+I+KGM++++ S WDD+VFI
Sbjct: 242 YRPVFFRKEDLEKSLIRASSQQNRLNPALKPGDIQVAVFEDIVKGMRESTTSNWDDIVFI 301

Query: 347 PPGFDVSTDPKKQ 349
           PPGF+VST+  ++
Sbjct: 302 PPGFEVSTEQTQE 313

BLAST of Lag0001824 vs. TAIR 10
Match: AT4G33350.1 (Tic22-like family protein )

HSP 1 Score: 147.9 bits (372), Expect = 1.5e-35
Identity = 93/251 (37.05%), Postives = 139/251 (55.38%), Query Frame = 0

Query: 92  TQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTEAI 151
           T+  S  S+     KR  ++  +    +   P +A V++      S S      LS   +
Sbjct: 22  TRFSSDLSARIEDTKRFAETLATRRFSLPTPPPFASVSQ------SKSGTPTTTLSPSLV 81

Query: 152 EERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNGSK 211
            + LAG  V+ +SN   EFVL+S  +  KS+GL CF++EDAEA L          +  +K
Sbjct: 82  AKALAGTSVFTVSNTNNEFVLISDPTGGKSIGLLCFRQEDAEAFLAQARLRRRELKTNAK 141

Query: 212 VVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLILRS 271
           VVP+ L++V+ L V G++FR +P+  Q+KNAL E + +G + +GF GVPVFQS+ L++R 
Sbjct: 142 VVPITLDQVYLLKVEGISFRFLPDPIQIKNAL-ELKSSG-NKNGFDGVPVFQSELLVVRK 201

Query: 272 QNKSYRPAFFRKEDLENSLQ---RASREQNQINPAFRPGDIQVAVFEEILKGMK-DNSIS 331
           +N+ Y P +F KED+E  L    RASR   Q         I V   E++L+ M+     S
Sbjct: 202 KNRRYCPVYFSKEDIERELSKYTRASRGDQQ---------IMVGSLEDVLRKMEMSEKNS 255

Query: 332 TWDDVVFIPPG 339
            W+DV+FIPPG
Sbjct: 262 GWEDVIFIPPG 255

BLAST of Lag0001824 vs. TAIR 10
Match: AT4G33350.2 (Tic22-like family protein )

HSP 1 Score: 122.9 bits (307), Expect = 5.2e-28
Identity = 81/248 (32.66%), Postives = 125/248 (50.40%), Query Frame = 0

Query: 92  TQLQSTFSSLQNQAKRALDSRISAFDPVAKNPVWARVAETAKTQFSPSARLGVALSTEAI 151
           T+  S  S+     KR  ++  +    +   P +A V++      S S      LS   +
Sbjct: 22  TRFSSDLSARIEDTKRFAETLATRRFSLPTPPPFASVSQ------SKSGTPTTTLSPSLV 81

Query: 152 EERLAGIPVYALSNAAEEFVLVSGASTAKSLGLFCFKKEDAEALLEHIGTMDASARNGSK 211
            + LAG  V+ +SN   EFVL+S  +  KS+GL CF++EDAEA L          +  +K
Sbjct: 82  AKALAGTSVFTVSNTNNEFVLISDPTGGKSIGLLCFRQEDAEAFLAQARLRRRELKTNAK 141

Query: 212 VVPVALNKVFQLHVNGVAFRLIPECSQVKNALSEREKAGISADGFPGVPVFQSKSLILRS 271
           VVP+ L++V+ L V G++FR +P+  Q+KNAL E + +G + +GF GVPVFQS+ L++R 
Sbjct: 142 VVPITLDQVYLLKVEGISFRFLPDPIQIKNAL-ELKSSG-NKNGFDGVPVFQSELLVVRK 201

Query: 272 QNKSYRPAFFRKEDLENSLQRASREQNQINPAFRPGDIQVAVFEEILKGMK-DNSISTWD 331
           +N+ Y P                                V   E++L+ M+     S W+
Sbjct: 202 KNRRYCP--------------------------------VGSLEDVLRKMEMSEKNSGWE 229

Query: 332 DVVFIPPG 339
           DV+FIPPG
Sbjct: 262 DVIFIPPG 229

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022959990.19.8e-15488.75protein TIC 22-like, chloroplastic [Cucurbita moschata][more]
XP_023005035.18.3e-15388.13protein TIC 22-like, chloroplastic [Cucurbita maxima][more]
XP_023514114.15.4e-15287.81protein TIC 22-like, chloroplastic [Cucurbita pepo subsp. pepo][more]
KAG7025300.13.6e-14888.10Protein TIC 22-like, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6592897.14.0e-14787.78Protein TIC 22-like, chloroplastic, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
F4J4692.0e-8557.51Protein TIC 22-like, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC22L PE=... [more]
Q9ZST95.4e-3844.33Protein TIC 22, chloroplastic OS=Pisum sativum OX=3888 GN=TIC22 PE=1 SV=1[more]
Q9SZB22.1e-3437.05Protein TIC 22, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC22 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H7U64.8e-15488.75protein TIC 22-like, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460879... [more]
A0A6J1KWA04.0e-15388.13protein TIC 22-like, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111498148 P... [more]
A0A6J1D9F74.8e-13876.99protein TIC 22-like, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1DA192.3e-12472.16protein TIC 22-like, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=... [more]
A0A5D3BU871.0e-11977.32Protein TIC 22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold128G... [more]
Match NameE-valueIdentityDescription
AT3G23710.11.4e-8657.51Tic22-like family protein [more]
AT4G33350.11.5e-3537.05Tic22-like family protein [more]
AT4G33350.25.2e-2832.66Tic22-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007378Tic22-likePFAMPF04278Tic22coord: 97..344
e-value: 4.1E-89
score: 298.0
IPR007378Tic22-likePANTHERPTHR33926PROTEIN TIC 22, CHLOROPLASTICcoord: 60..347
NoneNo IPR availableGENE3D3.40.1350.100coord: 150..233
e-value: 2.6E-15
score: 58.7
NoneNo IPR availableGENE3D3.40.1350.100coord: 234..303
e-value: 2.6E-15
score: 58.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 19..33
NoneNo IPR availablePANTHERPTHR33926:SF1PROTEIN TIC 22-LIKE, CHLOROPLASTICcoord: 60..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0001824.1Lag0001824.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
cellular_component GO:0009507 chloroplast