Lag0001805 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0001805
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	rRNA N-glycosidase
Location	chr4: 35588173 .. 35589000 (-)
RNA-Seq Expression	Lag0001805
Synteny	Lag0001805

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGATGAAGAAATTGGTTGGGTTTTGCTTTGTTGTGGCTCTCTATCTTGGTGCTCATACCACAGAGGCTAATGTCAGCTTCTCTCTATTGGGTGCCACTAGTGCAAGCTACAGACAGTTCATAAACAATCTGCGCACCACACTCACGGTCGGAACTCGAAGAGTGTACAATATACCTGTGTTGAGGAACGGAACAGCAGGGGCGGCGCGATACCTCTTAATGCAGCTCACGAATCTTAATCGTGAATCTATTACTATGGCACTGGATGTGATGGACGTGTATGTTGTTGGATATCAAGCTGGAAATACTCGCTACTTTCTCAATGATACGCCAGCAGGAGCGGCCAATGTGCTATTTACAGGCTCCAATAGAACGGTAAGATTTTCATTTGGAGGCAATTATGATGGCCTTGAGAGGGCTGCAAGGGCAAGTAGAGAGAATATTGACCTTGGATTTTCCGTAATGAGTAGTTCCATTGGTAACATGTTTCGCAACGCTGCCGGTACTTCTGTCCCTAGAGCATTTATTATCATCATCCAAATGGTCTCTGAAGCTGCAAGATTTAAATACATTGAGCAAAGAGTTTCTCAAAGTGTTCAAACCACGTTTCGGCCTGACCCTGCATTTTTAAGCTTACAAAATAATTGGGGCAGTCTCTCTGAACAAATACTGATTGCTGAATTTCGAGGAGGGAATTTTAGTCGTCCAGTTGAGCTTCGAAGTGTCAGCAACAATATAGTTATCGTGAACAATGTTAATTCGCCTGTTGTGCAAGGGATTGCACTTCTAAAACACTTCAATGTTAGTGTTCCCATGCATTGA

mRNA sequence

ATGGAAAAGATGAAGAAATTGGTTGGGTTTTGCTTTGTTGTGGCTCTCTATCTTGGTGCTCATACCACAGAGGCTAATGTCAGCTTCTCTCTATTGGGTGCCACTAGTGCAAGCTACAGACAGTTCATAAACAATCTGCGCACCACACTCACGGTCGGAACTCGAAGAGTGTACAATATACCTGTGTTGAGGAACGGAACAGCAGGGGCGGCGCGATACCTCTTAATGCAGCTCACGAATCTTAATCGTGAATCTATTACTATGGCACTGGATGTGATGGACGTGTATGTTGTTGGATATCAAGCTGGAAATACTCGCTACTTTCTCAATGATACGCCAGCAGGAGCGGCCAATGTGCTATTTACAGGCTCCAATAGAACGGTAAGATTTTCATTTGGAGGCAATTATGATGGCCTTGAGAGGGCTGCAAGGGCAAGTAGAGAGAATATTGACCTTGGATTTTCCGTAATGAGTAGTTCCATTGGTAACATGTTTCGCAACGCTGCCGGTACTTCTGTCCCTAGAGCATTTATTATCATCATCCAAATGGTCTCTGAAGCTGCAAGATTTAAATACATTGAGCAAAGAGTTTCTCAAAGTGTTCAAACCACGTTTCGGCCTGACCCTGCATTTTTAAGCTTACAAAATAATTGGGGCAGTCTCTCTGAACAAATACTGATTGCTGAATTTCGAGGAGGGAATTTTAGTCGTCCAGTTGAGCTTCGAAGTGTCAGCAACAATATAGTTATCGTGAACAATGTTAATTCGCCTGTTGTGCAAGGGATTGCACTTCTAAAACACTTCAATGTTAGTGTTCCCATGCATTGA

Coding sequence (CDS)

ATGGAAAAGATGAAGAAATTGGTTGGGTTTTGCTTTGTTGTGGCTCTCTATCTTGGTGCTCATACCACAGAGGCTAATGTCAGCTTCTCTCTATTGGGTGCCACTAGTGCAAGCTACAGACAGTTCATAAACAATCTGCGCACCACACTCACGGTCGGAACTCGAAGAGTGTACAATATACCTGTGTTGAGGAACGGAACAGCAGGGGCGGCGCGATACCTCTTAATGCAGCTCACGAATCTTAATCGTGAATCTATTACTATGGCACTGGATGTGATGGACGTGTATGTTGTTGGATATCAAGCTGGAAATACTCGCTACTTTCTCAATGATACGCCAGCAGGAGCGGCCAATGTGCTATTTACAGGCTCCAATAGAACGGTAAGATTTTCATTTGGAGGCAATTATGATGGCCTTGAGAGGGCTGCAAGGGCAAGTAGAGAGAATATTGACCTTGGATTTTCCGTAATGAGTAGTTCCATTGGTAACATGTTTCGCAACGCTGCCGGTACTTCTGTCCCTAGAGCATTTATTATCATCATCCAAATGGTCTCTGAAGCTGCAAGATTTAAATACATTGAGCAAAGAGTTTCTCAAAGTGTTCAAACCACGTTTCGGCCTGACCCTGCATTTTTAAGCTTACAAAATAATTGGGGCAGTCTCTCTGAACAAATACTGATTGCTGAATTTCGAGGAGGGAATTTTAGTCGTCCAGTTGAGCTTCGAAGTGTCAGCAACAATATAGTTATCGTGAACAATGTTAATTCGCCTGTTGTGCAAGGGATTGCACTTCTAAAACACTTCAATGTTAGTGTTCCCATGCATTGA

Protein sequence

MEKMKKLVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRNGTAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSNRTVRFSFGGNYDGLERAARASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVSEAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVSNNIVIVNNVNSPVVQGIALLKHFNVSVPMH

Homology

[Close]

BLAST of Lag0001805 vs. NCBI nr
Match: P98184.1 (RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica])

HSP 1 Score: 340.9 bits (873), Expect = 1.0e-89
Identity = 184/267 (68.91%), Postives = 216/267 (80.90%), Query Frame = 0


Query: 8   VGFCFVVALYLGAHTTE-ANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRNG 67
           +GF  V+ALY+GAH TE  +++FSL+GAT A+Y+ FI NLRT LTVGT RVY+IPVLRN 
Sbjct: 4   IGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNA 63

Query: 68  TAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSNR 127
            AG AR+ L+ LTN N ES+T+ALDV++VYVV Y+AGNT YFL D    A NVLF G N 
Sbjct: 64  AAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINH 123

Query: 128 TVRFSFGGNYDGLERAA-RASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVS 187
            VR  +GGNYDGLE AA R SRENI+LGFS +SS+IGNMFR+  GTSVPRAFI+IIQ VS
Sbjct: 124 -VRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVS 183

Query: 188 EAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVS 247
           EAARFKYIEQRVS++V T F+PDPAFLSLQN WGSLSEQI IA+ RGG F+RPVELR+VS
Sbjct: 184 EAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVS 243

Query: 248 NNIVIVNNVNSPVVQGIALLKHFNVSV 273
           N    V NVNSPVV+GIALL +F V+V
Sbjct: 244 NTPTFVTNVNSPVVKGIALLLYFRVNV 269

[Close]

BLAST of Lag0001805 vs. NCBI nr
Match: BAG12408.1 (karasurin-H precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 334.3 bits (856), Expect = 9.7e-88
Identity = 179/268 (66.79%), Postives = 215/268 (80.22%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRN 65
           KLVGF  V+ALY+GA  TEA+V+FSLLG+TSA+Y+QFI NLR  LTVG+  V NIPVL+ 
Sbjct: 2   KLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKG 61

Query: 66  GTAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSN 125
             AG AR+LL+ LTN N ESIT+A+DV++VYVV Y+AGN  YFLND  A A NVLF G N
Sbjct: 62  TAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGIN 121

Query: 126 RTVRFSFGGNYDGLERAA-RASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMV 185
             VR  +GGNYDGLE AA R SRE I LGFS +SS+IG+MF   AGTSVP+AFI+IIQ V
Sbjct: 122 H-VRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 181

Query: 186 SEAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSV 245
           SEAARFKYI+QRVS++VQT F PDPAFLSL+N WG LSEQI IA++RGG F+RP+ELR+V
Sbjct: 182 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 241

Query: 246 SNNIVIVNNVNSPVVQGIALLKHFNVSV 273
           SN    V NVNSPVV+G+ALL ++ V+V
Sbjct: 242 SNTPTFVTNVNSPVVKGVALLLYYKVNV 268

[Close]

BLAST of Lag0001805 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 331.6 bits (849), Expect = 6.3e-87
Identity = 175/269 (65.06%), Postives = 217/269 (80.67%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRN 65
           +L+ F F +ALYLGA   EA+VSFS+LG+TS +Y+QFI N+R+ LTVG++ VY+IPVL+ 
Sbjct: 2   RLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKA 61

Query: 66  GTAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSN 125
              G+AR+LL+ LTN N ESI +A+DV++VYVV Y+AGN  YFLND  A A  VLF G N
Sbjct: 62  TATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGIN 121

Query: 126 RTVRFSFGGNYDGLERAARASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVS 185
             V+ S+ GNYDGLE AA+ SRE IDLGFS +SSSIGNMF + AGTSVPRAFI+IIQ VS
Sbjct: 122 H-VKLSYKGNYDGLETAAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVS 181

Query: 186 EAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVS 245
           EAARFKYIEQRVS++V+TTF+PDPAFLSLQN W  LSEQI IA+ RGG F+R VELR+VS
Sbjct: 182 EAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVS 241

Query: 246 NNIVIVNNVNSPVVQGIALLKHFNVSVPM 275
           N  ++V +V+SPVV+GIALL ++ VSV M
Sbjct: 242 NKPILVTDVSSPVVKGIALLLYYKVSVVM 269

[Close]

BLAST of Lag0001805 vs. NCBI nr
Match: XP_022158192.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 307.8 bits (787), Expect = 9.7e-80
Identity = 165/268 (61.57%), Postives = 208/268 (77.61%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRN 65
           + + F FV+AL  GA   EA+VSFS+LGATS +YRQFI NLR+TLT+ +  VY IPVL++
Sbjct: 2   RFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQS 61

Query: 66  GTAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSN 125
              G+AR++L+ LTN N ESIT+A+DV+++YVV Y+AGN  YFLND  A A NVLF G  
Sbjct: 62  TAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI- 121

Query: 126 RTVRFSFGGNYDGLERAA-RASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMV 185
           + VR  + GNYDGLE AA + SRE IDLGFS +SSSIGNMF +  GTS+ RAFI+IIQ V
Sbjct: 122 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 181

Query: 186 SEAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSV 245
            EAARFKYIEQRVS++V+T F+PDPAFLSL+N W  LSEQI IA+ R G F+R +ELR+V
Sbjct: 182 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 241

Query: 246 SNNIVIVNNVNSPVVQGIALLKHFNVSV 273
           SN  +IV NV+SP+V+GIALL ++ V V
Sbjct: 242 SNKPIIVTNVSSPIVKGIALLLYYKVRV 268

[Close]

BLAST of Lag0001805 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 272.3 bits (695), Expect = 4.5e-69
Identity = 141/242 (58.26%), Postives = 183/242 (75.62%), Query Frame = 0


Query: 25  ANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRNGTAGAARYLLMQLTNLNRE 84
           +NVSFSLLGAT  +Y+QFI NLR  LT+ ++ VYNIPVL      +AR++L+ LTN   E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 85  SITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSNRTVRFSFGGNYDGLERAA- 144
           +IT+A+DV++VY+V YQAGN  YFL D    A +VLF G  + +   + GNYDGLE AA 
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEI-LPYKGNYDGLETAAG 154

Query: 145 RASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVSEAARFKYIEQRVSQSVQT 204
           + SRE IDLGFS + S+IGNM+   AGTSVPRAFI++IQ +SEAARFKYIE +VSQ+V+T
Sbjct: 155 KISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVET 214

Query: 205 TFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVSNNIVIVNNVNSPVVQGIA 264
            F+PDP FLSL+N W  LSEQ+ IA+ R G F+RP+E+RSV+N  ++V NV S VV+G+A
Sbjct: 215 KFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMA 274

Query: 265 LL 266
           LL
Sbjct: 275 LL 275

[Close]

BLAST of Lag0001805 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.4e-92
Identity = 184/267 (68.91%), Postives = 216/267 (80.90%), Query Frame = 0


Query: 8   VGFCFVVALYLGAHTTE-ANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRNG 67
           +GF  V+ALY+GAH TE  +++FSL+GAT A+Y+ FI NLRT LTVGT RVY+IPVLRN 
Sbjct: 4   IGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNA 63

Query: 68  TAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSNR 127
            AG AR+ L+ LTN N ES+T+ALDV++VYVV Y+AGNT YFL D    A NVLF G N 
Sbjct: 64  AAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINH 123

Query: 128 TVRFSFGGNYDGLERAA-RASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVS 187
            VR  +GGNYDGLE AA R SRENI+LGFS +SS+IGNMFR+  GTSVPRAFI+IIQ VS
Sbjct: 124 -VRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVS 183

Query: 188 EAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVS 247
           EAARFKYIEQRVS++V T F+PDPAFLSLQN WGSLSEQI IA+ RGG F+RPVELR+VS
Sbjct: 184 EAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVS 243

Query: 248 NNIVIVNNVNSPVVQGIALLKHFNVSV 273
           N    V NVNSPVV+GIALL +F V+V
Sbjct: 244 NTPTFVTNVNSPVVKGIALLLYFRVNV 269

[Close]

BLAST of Lag0001805 vs. ExPASy Swiss-Prot
Match: Q5F0I3 (Ribosome-inactivating protein gynostemmin OS=Gynostemma pentaphyllum OX=182084 PE=1 SV=2)

HSP 1 Score: 197.6 bits (501), Expect = 1.8e-49
Identity = 123/272 (45.22%), Postives = 174/272 (63.97%), Query Frame = 0


Query: 6   KLVGFCFVVA--LYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVL 65
           ++  FC VVA  LY G H  E +++FSL GA   +Y  FI  LR  L++GT++V NI VL
Sbjct: 2   RVARFCTVVAILLYFGFHIAECDINFSLAGADGQTYNTFIAKLRQELSIGTQKVANITVL 61

Query: 66  RNG-TAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAAN--VL 125
           ++  ++   R+L + L N N + IT+ +DV DVYVVG+  G   Y   + P  A N  +L
Sbjct: 62  KHHVSSNTQRFLSINLFNYNGDKITLGIDVFDVYVVGFLTGTNSYIFKEAPDLAYNKSLL 121

Query: 126 FTGSNRTVRFSFGGNYDGLERAARASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIII 185
           F GS R    S+ G YD LER   A RE+I LG   + ++I N+FR  + TS  R+FI+I
Sbjct: 122 FPGSVRE-NLSYTGGYDDLERRG-AGREDIPLGLLPLDTAITNLFRRDS-TSFRRSFIVI 181

Query: 186 IQMVSEAARFKYIEQRVSQSV--QTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRP 245
           IQMVSEAARFK IE ++++++  + TF+PD A +SL+NNWG+LS+QI  A+ RGG F   
Sbjct: 182 IQMVSEAARFKIIEAKIAKNLYGENTFKPDQAIISLENNWGALSKQIQKAQDRGGVFPNL 241

Query: 246 VELRSVSNNIVIVNNVNSPVVQ-GIALLKHFN 270
           V L + S   +I+ N + P+VQ GIALLK+ +
Sbjct: 242 VTLTTSSGKPLIIRNDSDPLVQNGIALLKYMS 270

[Close]

BLAST of Lag0001805 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 6.0e-48
Identity = 117/265 (44.15%), Postives = 165/265 (62.26%), Query Frame = 0


Query: 7   LVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRNG 66
           LV    ++ L+L A   E +VSF L GATS+SY  FI+NLR  L    R++Y+IP+LR+ 
Sbjct: 5   LVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPY-ERKLYDIPLLRST 64

Query: 67  TAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPA-GAANVLFTGSN 126
             G+ RY L+ LTN   E+I++A+DV +VYV+GY+AG+T YF N+  A  AA  +F  + 
Sbjct: 65  LPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFKDAK 124

Query: 127 RTVRFSFGGNYDGLERAARASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVS 186
           R V   + GNY+ L+ AA   RENI LG   + S+I  +F   A  S   A +++IQ  S
Sbjct: 125 RKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNA-NSAASALMVLIQSTS 184

Query: 187 EAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVS 246
           EAAR+K+IEQ++ + V  TF P  A +SL+N+W +LS+QI IA    G F  PV L +  
Sbjct: 185 EAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINAQ 244

Query: 247 NNIVIVNNVNSPVV-QGIALLKHFN 270
           N  V + NV++ VV   IALL + N
Sbjct: 245 NQRVTITNVDAGVVTSNIALLLNRN 267

[Close]

BLAST of Lag0001805 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 1.3e-47
Identity = 115/265 (43.40%), Postives = 164/265 (61.89%), Query Frame = 0


Query: 7   LVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRNG 66
           LV    ++ L+L     E +VSF L GATS+SY  FI+NLR  L    R++Y+IP+LR+ 
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSS 64

Query: 67  TAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPA-GAANVLFTGSN 126
             G+ RY L+ LTN   E+I++A+DV +VY++GY+AG+T YF N+  A  AA  +F  + 
Sbjct: 65  LPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAM 124

Query: 127 RTVRFSFGGNYDGLERAARASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVS 186
           R V   + GNY+ L+ AA   RENI LG   + S+I  +F   A  S   A +++IQ  S
Sbjct: 125 RKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNA-NSAASALMVLIQSTS 184

Query: 187 EAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVS 246
           EAAR+K+IEQ++ + V  TF P  A +SL+N+W +LS+QI IA    G F  PV L +  
Sbjct: 185 EAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQ 244

Query: 247 NNIVIVNNVNSPVV-QGIALLKHFN 270
           N  V + NV++ VV   IALL + N
Sbjct: 245 NQRVTITNVDAGVVTSNIALLLNRN 267

[Close]

BLAST of Lag0001805 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 190.7 bits (483), Expect = 2.3e-47
Identity = 112/268 (41.79%), Postives = 166/268 (61.94%), Query Frame = 0


Query: 4   MKKLVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVL 63
           +K LV +  ++ ++L + T E +VSF L GAT+ SY  FI NLR  L    R+VYNIP+L
Sbjct: 2   IKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPY-ERKVYNIPLL 61

Query: 64  RNGTAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPA-GAANVLFT 123
           R+  +G+ RY L+ LTN   E+I++A+DV +VY++GY AG+  YF N+  A  AA  +F 
Sbjct: 62  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFK 121

Query: 124 GSNRTVRFSFGGNYDGLERAARASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQ 183
            + + V   + GNY+ L+ AA   RENI LG   + S+I  ++   A +S   A +++IQ
Sbjct: 122 DAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTA-SSAASALLVLIQ 181

Query: 184 MVSEAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELR 243
             +E+AR+K+IEQ++ + V  TF P  A +SL+NNW +LS+QI IA    G F  PV L 
Sbjct: 182 STAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNGQFESPVVLI 241

Query: 244 SVSNNIVIVNNVNSPVV-QGIALLKHFN 270
             +N  V + N ++ VV   IALL + N
Sbjct: 242 DGNNQRVSITNASARVVTSNIALLLNRN 267

[Close]

BLAST of Lag0001805 vs. ExPASy TrEMBL
Match: B1B626 (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 4.7e-88
Identity = 179/268 (66.79%), Postives = 215/268 (80.22%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRN 65
           KLVGF  V+ALY+GA  TEA+V+FSLLG+TSA+Y+QFI NLR  LTVG+  V NIPVL+ 
Sbjct: 2   KLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKG 61

Query: 66  GTAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSN 125
             AG AR+LL+ LTN N ESIT+A+DV++VYVV Y+AGN  YFLND  A A NVLF G N
Sbjct: 62  TAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGIN 121

Query: 126 RTVRFSFGGNYDGLERAA-RASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMV 185
             VR  +GGNYDGLE AA R SRE I LGFS +SS+IG+MF   AGTSVP+AFI+IIQ V
Sbjct: 122 H-VRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 181

Query: 186 SEAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSV 245
           SEAARFKYI+QRVS++VQT F PDPAFLSL+N WG LSEQI IA++RGG F+RP+ELR+V
Sbjct: 182 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 241

Query: 246 SNNIVIVNNVNSPVVQGIALLKHFNVSV 273
           SN    V NVNSPVV+G+ALL ++ V+V
Sbjct: 242 SNTPTFVTNVNSPVVKGVALLLYYKVNV 268

[Close]

BLAST of Lag0001805 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 3.0e-87
Identity = 175/269 (65.06%), Postives = 217/269 (80.67%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRN 65
           +L+ F F +ALYLGA   EA+VSFS+LG+TS +Y+QFI N+R+ LTVG++ VY+IPVL+ 
Sbjct: 2   RLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKA 61

Query: 66  GTAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSN 125
              G+AR+LL+ LTN N ESI +A+DV++VYVV Y+AGN  YFLND  A A  VLF G N
Sbjct: 62  TATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGIN 121

Query: 126 RTVRFSFGGNYDGLERAARASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVS 185
             V+ S+ GNYDGLE AA+ SRE IDLGFS +SSSIGNMF + AGTSVPRAFI+IIQ VS
Sbjct: 122 H-VKLSYKGNYDGLETAAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVS 181

Query: 186 EAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVS 245
           EAARFKYIEQRVS++V+TTF+PDPAFLSLQN W  LSEQI IA+ RGG F+R VELR+VS
Sbjct: 182 EAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVS 241

Query: 246 NNIVIVNNVNSPVVQGIALLKHFNVSVPM 275
           N  ++V +V+SPVV+GIALL ++ VSV M
Sbjct: 242 NKPILVTDVSSPVVKGIALLLYYKVSVVM 269

[Close]

BLAST of Lag0001805 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 4.7e-80
Identity = 165/268 (61.57%), Postives = 208/268 (77.61%), Query Frame = 0


Query: 6   KLVGFCFVVALYLGAHTTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRN 65
           + + F FV+AL  GA   EA+VSFS+LGATS +YRQFI NLR+TLT+ +  VY IPVL++
Sbjct: 2   RFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQS 61

Query: 66  GTAGAARYLLMQLTNLNRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSN 125
              G+AR++L+ LTN N ESIT+A+DV+++YVV Y+AGN  YFLND  A A NVLF G  
Sbjct: 62  TAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI- 121

Query: 126 RTVRFSFGGNYDGLERAA-RASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMV 185
           + VR  + GNYDGLE AA + SRE IDLGFS +SSSIGNMF +  GTS+ RAFI+IIQ V
Sbjct: 122 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 181

Query: 186 SEAARFKYIEQRVSQSVQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSV 245
            EAARFKYIEQRVS++V+T F+PDPAFLSL+N W  LSEQI IA+ R G F+R +ELR+V
Sbjct: 182 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 241

Query: 246 SNNIVIVNNVNSPVVQGIALLKHFNVSV 273
           SN  +IV NV+SP+V+GIALL ++ V V
Sbjct: 242 SNKPIIVTNVSSPIVKGIALLLYYKVRV 268

[Close]

BLAST of Lag0001805 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 2.2e-69
Identity = 141/242 (58.26%), Postives = 183/242 (75.62%), Query Frame = 0


Query: 25  ANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRNGTAGAARYLLMQLTNLNRE 84
           +NVSFSLLGAT  +Y+QFI NLR  LT+ ++ VYNIPVL      +AR++L+ LTN   E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 85  SITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSNRTVRFSFGGNYDGLERAA- 144
           +IT+A+DV++VY+V YQAGN  YFL D    A +VLF G  + +   + GNYDGLE AA 
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEI-LPYKGNYDGLETAAG 154

Query: 145 RASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVSEAARFKYIEQRVSQSVQT 204
           + SRE IDLGFS + S+IGNM+   AGTSVPRAFI++IQ +SEAARFKYIE +VSQ+V+T
Sbjct: 155 KISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVET 214

Query: 205 TFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVSNNIVIVNNVNSPVVQGIA 264
            F+PDP FLSL+N W  LSEQ+ IA+ R G F+RP+E+RSV+N  ++V NV S VV+G+A
Sbjct: 215 KFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMA 274

Query: 265 LL 266
           LL
Sbjct: 275 LL 275

[Close]

BLAST of Lag0001805 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 3.7e-69
Identity = 143/245 (58.37%), Postives = 184/245 (75.10%), Query Frame = 0


Query: 22  TTEANVSFSLLGATSASYRQFINNLRTTLTVGTRRVYNIPVLRNGTAGAARYLLMQLTNL 81
           +  AN+SFSLLGAT+ +Y+QFIN+LR  LTV + +VY+IPVL     G AR++L+ LTN 
Sbjct: 31  SANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNY 90

Query: 82  NRESITMALDVMDVYVVGYQAGNTRYFLNDTPAGAANVLFTGSNRTVRFSFGGNYDGLER 141
             ESIT+A+D +DVY+V YQAGN  YFL D    A +VLF GS      S+ GNYD LER
Sbjct: 91  KGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHET-LSYKGNYDDLER 150

Query: 142 -AARASRENIDLGFSVMSSSIGNMFRNAAGTSVPRAFIIIIQMVSEAARFKYIEQRVSQS 201
            A + SRENI+LGFS +SSSIGNMF   AG SVPR+FI++IQM+SEA+RFKYIE R  + 
Sbjct: 151 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 210

Query: 202 VQTTFRPDPAFLSLQNNWGSLSEQILIAEFRGGNFSRPVELRSVSNNIVIVNNVNSPVVQ 261
           V++ F+PDP +LSL+N W +LSEQ+  A+  GG F  PV++RSVSN  V+V++VNSPVV+
Sbjct: 211 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 270

Query: 262 GIALL 266
           GIALL
Sbjct: 271 GIALL 274

The following BLAST results are available for this feature:

BLAST of Lag0001805 vs. NCBI nr


Match Name	E-value	Identity	Description
P98184.1	1.0e-89	68.91	RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltNa...	[more]
BAG12408.1	9.7e-88	66.79	karasurin-H precursor [Trichosanthes kirilowii var. japonica]	[more]
XP_022158193.1	6.3e-87	65.06	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158192.1	9.7e-80	61.57	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158191.1	4.5e-69	58.26	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]

BLAST of Lag0001805 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	1.4e-92	68.91	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
Q5F0I3	1.8e-49	45.22	Ribosome-inactivating protein gynostemmin OS=Gynostemma pentaphyllum OX=182084 P...	[more]
P24478	6.0e-48	44.15	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P09989	1.3e-47	43.40	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P33185	2.3e-47	41.79	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]

BLAST of Lag0001805 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
B1B626	4.7e-88	66.79	rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1	[more]
A0A6J1DVE7	3.0e-87	65.06	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]
A0A6J1DWK4	4.7e-80	61.57	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1E093	2.2e-69	58.26	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1EKL0	3.7e-69	58.37	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1	[more]

BLAST of Lag0001805 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 37..50 score: 42.48 coord: 177..197 score: 61.28 coord: 147..161 score: 42.11 coord: 209..226 score: 41.96 coord: 85..100 score: 48.03
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 190..272 e-value: 1.6E-25 score: 90.7
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 26..189 e-value: 1.1E-43 score: 150.8
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 35..226 e-value: 7.4E-35 score: 120.4
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 21..266
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 21..266
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 181..197
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 27..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0001805.1	Lag0001805.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity