Lag0001803 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0001803
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	rRNA N-glycosidase
Location	chr4: 35575793 .. 35576650 (-)
RNA-Seq Expression	Lag0001803
Synteny	Lag0001803

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGAAGATGACAGCAATTGTTGTATGTTTTGTTCTGGCTGCTTCACTCGCCAGCATCCATGGGGTCTCGGCGGCTCATAAAATCATCCTCGGTAGATCGACGCATATCATCGGTCAAGATGGGCTGTGTTTGGACGTGATCGGAGGATACAACGACGATCATATTCCAACTCAGATATGGCCATGCGGACCTCAAAACAACCAACTATGGACGATTCGAACCGACGGGACGGTTCGAGCCATGGGAAAATGCTTGGTTCCCAATGGCAATGACCCCGGATCCTACACCATGATAAACAATTGCGATACCTCCGCACCCGATGACAAAACTTGGAAACTATGGCTCGATGGAACCCTAACCCACGTCCACTCGGGCCTGGTCTTGACGGCTCATGCAGCCGGGCCACACCCCATAACCACAATAGAGAGCAACACATACGCTACCGCGCAGAGTTGGGACACGGCGGAGAACGGGGCACCGGTCGTGACGGCGATCGTCGGGATCCGCCGCATGTGCCTGCAGGCTCAGAATGACAATGTCCATGTTTGGTTGAATAAATGCGTCAAAAGCGATAGAAAACAGTATTGGGCATTTTACGGCGATGGCACCATAAGAGCCAACTACAACACAAGCTTGTGCGTAACTGGTGGCGGCAAAGGCTTGAACGATGCAGTGGTTTTGGGTAAGTGTGAGGGCTCAGCTCAGCAGCGATGGGTAGCCAAAGAAGATGGCACGATCTTAAACCCAGGTACTAATTTGGTGATGGATGTGAAAGGAAGTGATCCTAATCTTCGACAGATTATTCTGTTTTCGGCTACTGGTAATCCTAACCAGCGCTGGATGTTTCCTTAG

mRNA sequence

ATGGAGAAGAAGATGACAGCAATTGTTGTATGTTTTGTTCTGGCTGCTTCACTCGCCAGCATCCATGGGGTCTCGGCGGCTCATAAAATCATCCTCGGTAGATCGACGCATATCATCGGTCAAGATGGGCTGTGTTTGGACGTGATCGGAGGATACAACGACGATCATATTCCAACTCAGATATGGCCATGCGGACCTCAAAACAACCAACTATGGACGATTCGAACCGACGGGACGGTTCGAGCCATGGGAAAATGCTTGGTTCCCAATGGCAATGACCCCGGATCCTACACCATGATAAACAATTGCGATACCTCCGCACCCGATGACAAAACTTGGAAACTATGGCTCGATGGAACCCTAACCCACGTCCACTCGGGCCTGGTCTTGACGGCTCATGCAGCCGGGCCACACCCCATAACCACAATAGAGAGCAACACATACGCTACCGCGCAGAGTTGGGACACGGCGGAGAACGGGGCACCGGTCGTGACGGCGATCGTCGGGATCCGCCGCATGTGCCTGCAGGCTCAGAATGACAATGTCCATGTTTGGTTGAATAAATGCGTCAAAAGCGATAGAAAACAGTATTGGGCATTTTACGGCGATGGCACCATAAGAGCCAACTACAACACAAGCTTGTGCGTAACTGGTGGCGGCAAAGGCTTGAACGATGCAGTGGTTTTGGGTAAGTGTGAGGGCTCAGCTCAGCAGCGATGGGTAGCCAAAGAAGATGGCACGATCTTAAACCCAGGTACTAATTTGGTGATGGATGTGAAAGGAAGTGATCCTAATCTTCGACAGATTATTCTGTTTTCGGCTACTGGTAATCCTAACCAGCGCTGGATGTTTCCTTAG

Coding sequence (CDS)

ATGGAGAAGAAGATGACAGCAATTGTTGTATGTTTTGTTCTGGCTGCTTCACTCGCCAGCATCCATGGGGTCTCGGCGGCTCATAAAATCATCCTCGGTAGATCGACGCATATCATCGGTCAAGATGGGCTGTGTTTGGACGTGATCGGAGGATACAACGACGATCATATTCCAACTCAGATATGGCCATGCGGACCTCAAAACAACCAACTATGGACGATTCGAACCGACGGGACGGTTCGAGCCATGGGAAAATGCTTGGTTCCCAATGGCAATGACCCCGGATCCTACACCATGATAAACAATTGCGATACCTCCGCACCCGATGACAAAACTTGGAAACTATGGCTCGATGGAACCCTAACCCACGTCCACTCGGGCCTGGTCTTGACGGCTCATGCAGCCGGGCCACACCCCATAACCACAATAGAGAGCAACACATACGCTACCGCGCAGAGTTGGGACACGGCGGAGAACGGGGCACCGGTCGTGACGGCGATCGTCGGGATCCGCCGCATGTGCCTGCAGGCTCAGAATGACAATGTCCATGTTTGGTTGAATAAATGCGTCAAAAGCGATAGAAAACAGTATTGGGCATTTTACGGCGATGGCACCATAAGAGCCAACTACAACACAAGCTTGTGCGTAACTGGTGGCGGCAAAGGCTTGAACGATGCAGTGGTTTTGGGTAAGTGTGAGGGCTCAGCTCAGCAGCGATGGGTAGCCAAAGAAGATGGCACGATCTTAAACCCAGGTACTAATTTGGTGATGGATGTGAAAGGAAGTGATCCTAATCTTCGACAGATTATTCTGTTTTCGGCTACTGGTAATCCTAACCAGCGCTGGATGTTTCCTTAG

Protein sequence

MEKKMTAIVVCFVLAASLASIHGVSAAHKIILGRSTHIIGQDGLCLDVIGGYNDDHIPTQIWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSYTMINNCDTSAPDDKTWKLWLDGTLTHVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTAENGAPVVTAIVGIRRMCLQAQNDNVHVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVTGGGKGLNDAVVLGKCEGSAQQRWVAKEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGNPNQRWMFP

Homology

[Close]

BLAST of Lag0001803 vs. NCBI nr
Match: XP_022150387.1 (nigrin b-like [Momordica charantia] >XP_022156716.1 nigrin b-like [Momordica charantia])

HSP 1 Score: 468.4 bits (1204), Expect = 4.4e-128
Identity = 213/284 (75.00%), Postives = 246/284 (86.62%), Query Frame = 0


Query: 1   MEKKMTAIVVCFVLAASLASIHGVSAAHKIILGRSTHIIGQDGLCLDVIGGYNDDHIPTQ 60
           MEKKM  IV+C VLAASL  IH VSAAH I+  RSTHI+G+DGLCLDVIGGYND+HIPTQ
Sbjct: 1   MEKKMMTIVLCLVLAASLVGIHHVSAAHTIVFSRSTHIVGRDGLCLDVIGGYNDNHIPTQ 60

Query: 61  IWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSYTMINNCDTSAPDDKTWKLWLDGT 120
           +W C PQN+QLWTI+TDGT+R+MGKCLVPNG  PGSYTMI++C+ + PDDKTWKL+ DGT
Sbjct: 61  LWTCNPQNHQLWTIQTDGTIRSMGKCLVPNGRSPGSYTMIDDCNDANPDDKTWKLYPDGT 120

Query: 121 LTHVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTAENGAPVVTAIVGIRRMCLQAQND 180
           L HV SGLVLTA A GPH ITTIE+NTYA  QSW T ++G P+VT IVG+RRMCLQAQND
Sbjct: 121 LAHVRSGLVLTAQATGPHTITTIETNTYAAMQSWTTGDDGTPIVTTIVGLRRMCLQAQND 180

Query: 181 NVHVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVTGGGKGLNDAVVLGKCEGSAQQRW 240
           NVHV +N C+K++R+QYWA YGDGTIRAN+NTSLC++G GKG NDA+VL KCEGS QQRW
Sbjct: 181 NVHVSMNSCMKNNRQQYWALYGDGTIRANFNTSLCMSGTGKGSNDAIVLAKCEGSPQQRW 240

Query: 241 VAKEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGNPNQRWMF 285
           +AKEDGTILN  TNLVMDVKGSDPNLRQIILF  TGNPNQ+W++
Sbjct: 241 LAKEDGTILNLNTNLVMDVKGSDPNLRQIILFGRTGNPNQQWIW 284

[Close]

BLAST of Lag0001803 vs. NCBI nr
Match: XP_016903609.1 (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 452.6 bits (1163), Expect = 2.5e-123
Identity = 204/282 (72.34%), Postives = 240/282 (85.11%), Query Frame = 0


Query: 3   KKMTAIVVCFVLAASLASIHGVSAAHKIILGRSTHIIGQDGLCLDVIGGYNDDHIPTQIW 62
           K  T I VC VLAASL  IH VSAA  I+L RSTH++GQDGLCLDVIGGY+D+H+PTQ+W
Sbjct: 4   KSTTIIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPTQLW 63

Query: 63  PCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSYTMINNCDTSAPDDKTWKLWLDGTLT 122
           PCGPQNNQLWTI+ DGT+R MGKCLVPNG+DPGSYTMI++C+ + P+DKTWKL+ DGTLT
Sbjct: 64  PCGPQNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDGTLT 123

Query: 123 HVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTAENGAPVVTAIVGIRRMCLQAQNDNV 182
           HV S LVLT+   G + ITTIE+NT A  QSW TA++G+P+V AIVG+RRMCLQAQNDN+
Sbjct: 124 HVRSSLVLTSQGTGAYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQNDNI 183

Query: 183 HVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVTGGGKGLNDAVVLGKCEGSAQQRWVA 242
           HVW+N CVK++R+QYWA + DGTIRANYNTS C++  GKG NDA+VL KCEG  QQRWVA
Sbjct: 184 HVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQRWVA 243

Query: 243 KEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGNPNQRWMF 285
           KEDGTIL+  TNLVMDVKGSDPNLR+IILF+ TGNPNQ WMF
Sbjct: 244 KEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMF 285

[Close]

BLAST of Lag0001803 vs. NCBI nr
Match: KAA0059853.1 (nigrin b-like [Cucumis melo var. makuwa] >TYK24807.1 nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 452.6 bits (1163), Expect = 2.5e-123
Identity = 204/282 (72.34%), Postives = 240/282 (85.11%), Query Frame = 0


Query: 3   KKMTAIVVCFVLAASLASIHGVSAAHKIILGRSTHIIGQDGLCLDVIGGYNDDHIPTQIW 62
           K  T I VC VLAASL  IH VSAA  I+L RSTH++GQDGLCLDVIGGY+D+H+PTQ+W
Sbjct: 9   KSTTIIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPTQLW 68

Query: 63  PCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSYTMINNCDTSAPDDKTWKLWLDGTLT 122
           PCGPQNNQLWTI+ DGT+R MGKCLVPNG+DPGSYTMI++C+ + P+DKTWKL+ DGTLT
Sbjct: 69  PCGPQNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDGTLT 128

Query: 123 HVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTAENGAPVVTAIVGIRRMCLQAQNDNV 182
           HV S LVLT+   G + ITTIE+NT A  QSW TA++G+P+V AIVG+RRMCLQAQNDN+
Sbjct: 129 HVRSSLVLTSQGTGAYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQNDNI 188

Query: 183 HVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVTGGGKGLNDAVVLGKCEGSAQQRWVA 242
           HVW+N CVK++R+QYWA + DGTIRANYNTS C++  GKG NDA+VL KCEG  QQRWVA
Sbjct: 189 HVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQRWVA 248

Query: 243 KEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGNPNQRWMF 285
           KEDGTIL+  TNLVMDVKGSDPNLR+IILF+ TGNPNQ WMF
Sbjct: 249 KEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMF 290

[Close]

BLAST of Lag0001803 vs. NCBI nr
Match: XP_038885951.1 (nigrin b-like [Benincasa hispida])

HSP 1 Score: 441.0 bits (1133), Expect = 7.6e-120
Identity = 199/282 (70.57%), Postives = 234/282 (82.98%), Query Frame = 0


Query: 3   KKMTAIVVCFVLAASLASIHGVSAAHKIILGRSTHIIGQDGLCLDVIGGYNDDHIPTQIW 62
           KKMT + +  VLAAS    H VSAA  I+L RSTH++G+DGLCLDVIGGY+D HIPTQ+W
Sbjct: 4   KKMTIVFLVVVLAASFTGNHDVSAARTIVLSRSTHLVGRDGLCLDVIGGYSDSHIPTQLW 63

Query: 63  PCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSYTMINNCDTSAPDDKTWKLWLDGTLT 122
           PCGPQNNQLWTI+ DGT+R MGKCLVPNG++PGSYTMI++C+     DKTWKL+ DGTL 
Sbjct: 64  PCGPQNNQLWTIQVDGTIRTMGKCLVPNGHEPGSYTMIDDCNKVDTQDKTWKLYPDGTLV 123

Query: 123 HVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTAENGAPVVTAIVGIRRMCLQAQNDNV 182
           HV SGLVLT    GPH ITTIE+NT A  QSW TA++G+P+V AIVG+R MCLQAQNDNV
Sbjct: 124 HVWSGLVLTGQGTGPHAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRHMCLQAQNDNV 183

Query: 183 HVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVTGGGKGLNDAVVLGKCEGSAQQRWVA 242
           HVW+N CVK++R+QYWA Y DGTIR NYNTS C++  GKGLNDA+ L KC+G  QQRWVA
Sbjct: 184 HVWMNSCVKNNRQQYWAVYNDGTIRTNYNTSFCLSSTGKGLNDAIGLAKCQGLPQQRWVA 243

Query: 243 KEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGNPNQRWMF 285
           KEDGTILNP  N+VMDVKGSDPNLR+IILF+ TGNPNQ+W+F
Sbjct: 244 KEDGTILNPINNMVMDVKGSDPNLRKIILFAPTGNPNQQWVF 285

[Close]

BLAST of Lag0001803 vs. NCBI nr
Match: KAG5543116.1 (hypothetical protein RHGRI_016012 [Rhododendron griersonianum])

HSP 1 Score: 288.5 bits (737), Expect = 6.3e-74
Identity = 133/250 (53.20%), Postives = 166/250 (66.40%), Query Frame = 0


Query: 35  STHIIGQDGLCLDVIGGYNDDHIPTQIWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDP 94
           STHI G+DGLC+DV    +DD  P Q+WPCG Q+NQ WT +TDGT+R++GKC+      P
Sbjct: 307 STHISGRDGLCVDVRDEKDDDGNPIQLWPCGQQSNQQWTFQTDGTIRSLGKCMTTYSYSP 366

Query: 95  GSYTMINNCDTSAPDDKTWKLWLDGTLTHVHSGLVLTAHAAGPHPITTIESNTYATAQSW 154
           G Y MI NC T+  D   W L  DGT+T+  SGLVLTA+ A      T+E+N +A  Q W
Sbjct: 367 GVYVMIFNCGTAVQDATKWSLSSDGTITNPRSGLVLTANQAAQGTTLTVETNVHAARQGW 426

Query: 155 D-TAENGAPVVTAIVGIRRMCLQAQNDNVHVWLNKCVKSDRKQYWAFYGDGTIRANYNTS 214
               E   P VT I+G+  +C+QA NDN  VWL  CV S ++Q WA YGDGTIR N N +
Sbjct: 427 RVVGEEVNPTVTTIIGLNDLCMQANNDNSRVWLESCVDSSQQQQWALYGDGTIRVNSNHN 486

Query: 215 LCVTGGGKGLNDAVVLGKCEGSAQQRWVAKEDGTILNPGTNLVMDVKGSDPNLRQIILFS 274
           LC+T  G    D +++ +C+G A QRWV K DGTILNP T LVMDVK SD +L QIIL+ 
Sbjct: 487 LCLTSDGHSSLDVIIILRCQGWADQRWVFKSDGTILNPNTKLVMDVKDSDVSLHQIILYQ 546

Query: 275 ATGNPNQRWM 284
            TGNPNQRW+
Sbjct: 547 PTGNPNQRWL 556

[Close]

BLAST of Lag0001803 vs. ExPASy Swiss-Prot
Match: Q9M653 (Ribosome-inactivating protein PMRIPt OS=Polygonatum multiflorum OX=45371 GN=RIPt PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.4e-68
Identity = 124/248 (50.00%), Postives = 157/248 (63.31%), Query Frame = 0


Query: 38  IIGQDGLCLDVIGGYNDDHIPTQIWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSY 97
           I G DGLC+DV    N+D IP Q+WPCG Q NQ WT  TDGT+++MGKC+  NG  PG Y
Sbjct: 344 ISGLDGLCMDVRNESNNDGIPIQLWPCGAQRNQQWTFHTDGTIQSMGKCMTSNGYHPGDY 403

Query: 98  TMINNCDTS-APDDKTWKLWLDGTLTHVHSGLVLTAHAAGPHPITTIESNTYATAQSWDT 157
            MI NC T+  PD   W + +DG++T+ HSGLVLTA  A    I  +  NT++  Q    
Sbjct: 404 VMIFNCSTAPVPDATKWVVSIDGSITNPHSGLVLTAPQAAQTTILLVVRNTHSAKQGRSV 463

Query: 158 AENGAPVVTAIVGIRRMCLQAQND-NVHVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLC 217
            ++  P+VT IVG + MCLQ  N+ N  VWL  C     +Q+WA Y DGTIR + + SLC
Sbjct: 464 GDDVEPIVTYIVGFKYMCLQGNNENNTRVWLEDCAVDRPQQWWALYSDGTIRVDSDRSLC 523

Query: 218 VTGGGKGLNDAVVLGKCEGSAQQRWVAKEDGTILNPGTNLVMDVKGSDPNLRQIILFSAT 277
           VT  G    DA+++  C+G   QR V   DGTILNP   LVMDV+ S+  LRQIIL+  T
Sbjct: 524 VTSDGHSSRDAIIILTCDGGINQRLVFNTDGTILNPNAQLVMDVRQSNVALRQIILYQPT 583

Query: 278 GNPNQRWM 284
           GNPNQ+WM
Sbjct: 584 GNPNQQWM 591

[Close]

BLAST of Lag0001803 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 256.5 bits (654), Expect = 3.5e-67
Identity = 114/247 (46.15%), Postives = 158/247 (63.97%), Query Frame = 0


Query: 37  HIIGQDGLCLDVIGGYNDDHIPTQIWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGS 96
           +I+G+DGLC+DV  GY+ D  P Q+WPCG Q NQ WT  +D T+R+MGKC+  NG + GS
Sbjct: 311 NIVGRDGLCVDVRNGYDTDGTPLQLWPCGTQRNQRWTFDSDDTIRSMGKCMTANGLNNGS 370

Query: 97  YTMINNCDTSAPDDKTWKLWLDGTLTHVHSGLVLTAHAAGPHPITTIESNTYATAQSWDT 156
             +I NC T+A +   W++ +DG++ +  SGLV+TA  A    I  +E N YA +Q W  
Sbjct: 371 NIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIYAASQGWTV 430

Query: 157 AENGAPVVTAIVGIRRMCLQAQNDNVHVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCV 216
             N  P+V +IVG + MCLQ+  +N  VW+  C  +  +Q WA YGD TIR N    LCV
Sbjct: 431 TNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYGDRTIRVNSTRGLCV 490

Query: 217 TGGGKGLNDAVVLGKCEGSAQQRWVAKEDGTILNPGTNLVMDVKGSDPNLRQIILFSATG 276
           T  G    D +++ KC+G   QRW    DG I+NP +  VMDV+ S+ +LR+II+F ATG
Sbjct: 491 TTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNVSLREIIIFPATG 550

Query: 277 NPNQRWM 284
           NPNQ+W+
Sbjct: 551 NPNQQWV 557

[Close]

BLAST of Lag0001803 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.2e-62
Identity = 121/246 (49.19%), Postives = 157/246 (63.82%), Query Frame = 0


Query: 38  IIGQDGLCLDVIGGYNDDHIPTQIWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSY 97
           I G DGLC+DV  G+  D  P Q+ PCG + NQLWT RTDGT+R +GKCL  +     S 
Sbjct: 325 ISGWDGLCVDVRYGHYIDGNPVQLRPCGNECNQLWTFRTDGTIRWLGKCLTAS-----SS 384

Query: 98  TMINNCDTSAPDDKTWKLWLDGTLTHVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTA 157
            MI +C+T  P+   W + +DGT+T+ HSGLVLTA  A      ++E+N +A  Q W T 
Sbjct: 385 VMIYDCNTVPPEATKWVVSIDGTITNPHSGLVLTAPQAAEGTALSLENNIHAARQGW-TV 444

Query: 158 ENGAPVVTAIVGIRRMCLQAQNDNVHVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVT 217
            +  P+VT IVG ++MCL+   +N  VWL  CV +  +Q WA YGDGTIR N N SLCVT
Sbjct: 445 GDVEPLVTFIVGYKQMCLRENGENNFVWLEDCVLNRVQQEWALYGDGTIRVNSNRSLCVT 504

Query: 218 GGGKGLNDAVVLGKCEGSAQQRWVAKEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGN 277
                 +D +V+ KCEGS  QRWV   +GTI NP   L+MDV   D +LR+IIL+  TGN
Sbjct: 505 SEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLLMDVAQRDVSLRKIILYRPTGN 564

Query: 278 PNQRWM 284
           PNQ+W+
Sbjct: 565 PNQQWI 564

[Close]

BLAST of Lag0001803 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 6.4e-61
Identity = 120/246 (48.78%), Postives = 154/246 (62.60%), Query Frame = 0


Query: 38  IIGQDGLCLDVIGGYNDDHIPTQIWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSY 97
           I G DGLC+DV  G+  D    Q+ PCG + NQLWT RTDGT+R +GKCL  +     S 
Sbjct: 325 ISGWDGLCVDVRDGHYIDGNTVQLGPCGNECNQLWTFRTDGTIRWLGKCLTTS-----SS 384

Query: 98  TMINNCDTSAPDDKTWKLWLDGTLTHVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTA 157
            MI +C+T  P+   W +  DGT+T+  SGLVLTA  A      ++E+N +A  Q W T 
Sbjct: 385 VMIYDCNTVPPEATKWVVSTDGTITNPRSGLVLTAPQAAEGTALSLENNIHAARQGW-TV 444

Query: 158 ENGAPVVTAIVGIRRMCLQAQNDNVHVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVT 217
            +  P+VT IVG ++MCL    +N  VWL  CV +  +Q WA YGDGTIR N N SLCVT
Sbjct: 445 GDVEPLVTFIVGYKQMCLTENGENNFVWLEDCVLNRVEQEWALYGDGTIRVNSNRSLCVT 504

Query: 218 GGGKGLNDAVVLGKCEGSAQQRWVAKEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGN 277
                 +D +V+ KCEGS  QRWV   +GTI NP   LVMDV  S+ +LR+IIL+  TGN
Sbjct: 505 SEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLVMDVAQSNVSLRKIILYPPTGN 564

Query: 278 PNQRWM 284
           PNQ+W+
Sbjct: 565 PNQQWI 564

[Close]

BLAST of Lag0001803 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.4e-54
Identity = 113/247 (45.75%), Postives = 145/247 (58.70%), Query Frame = 0


Query: 40  GQDGLCLDVIGGYNDDHIPTQIWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSYTM 99
           G+DG C +V  G   D  P Q+  CG Q+NQ WT  TDGT++++GKCL  +     S  M
Sbjct: 323 GRDGFCAEVKNGDEKDGTPVQLSSCGEQSNQQWTFSTDGTIQSLGKCLTTS-----SSVM 382

Query: 100 INNCDTSAPDDKTWKLWLDGTLTHVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTAEN 159
           I NC    P+   W + +DGT+T+  SGLVLTA  A    + ++E N +A  Q W    N
Sbjct: 383 IYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAARQGW-IVGN 442

Query: 160 GAPVVTAIVGIRRMCLQAQNDNVHVWLNKC-VKSDRK--QYWAFYGDGTIRANYNTSLCV 219
             P+VT IVG  +MCL+    N  V L  C VKS  K  Q WA YGDGTIR N + SLCV
Sbjct: 443 VEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWALYGDGTIRVNNDRSLCV 502

Query: 220 TGGGKGLNDAVVLGKCEGSAQQRWVAKEDGTILNPGTNLVMDVKGSDPNLRQIILFSATG 279
           T  GK  N+ +++ KC G A QRWV   DGTI NP + LVM V  +D  LR+IIL   +G
Sbjct: 503 TSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNPDSKLVMHVDQNDVPLRKIILSHPSG 562

Query: 280 NPNQRWM 284
             NQ+W+
Sbjct: 563 TSNQQWI 563

[Close]

BLAST of Lag0001803 vs. ExPASy TrEMBL
Match: A0A6J1DVT2 (nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111023562 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.2e-128
Identity = 213/284 (75.00%), Postives = 246/284 (86.62%), Query Frame = 0


Query: 1   MEKKMTAIVVCFVLAASLASIHGVSAAHKIILGRSTHIIGQDGLCLDVIGGYNDDHIPTQ 60
           MEKKM  IV+C VLAASL  IH VSAAH I+  RSTHI+G+DGLCLDVIGGYND+HIPTQ
Sbjct: 1   MEKKMMTIVLCLVLAASLVGIHHVSAAHTIVFSRSTHIVGRDGLCLDVIGGYNDNHIPTQ 60

Query: 61  IWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSYTMINNCDTSAPDDKTWKLWLDGT 120
           +W C PQN+QLWTI+TDGT+R+MGKCLVPNG  PGSYTMI++C+ + PDDKTWKL+ DGT
Sbjct: 61  LWTCNPQNHQLWTIQTDGTIRSMGKCLVPNGRSPGSYTMIDDCNDANPDDKTWKLYPDGT 120

Query: 121 LTHVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTAENGAPVVTAIVGIRRMCLQAQND 180
           L HV SGLVLTA A GPH ITTIE+NTYA  QSW T ++G P+VT IVG+RRMCLQAQND
Sbjct: 121 LAHVRSGLVLTAQATGPHTITTIETNTYAAMQSWTTGDDGTPIVTTIVGLRRMCLQAQND 180

Query: 181 NVHVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVTGGGKGLNDAVVLGKCEGSAQQRW 240
           NVHV +N C+K++R+QYWA YGDGTIRAN+NTSLC++G GKG NDA+VL KCEGS QQRW
Sbjct: 181 NVHVSMNSCMKNNRQQYWALYGDGTIRANFNTSLCMSGTGKGSNDAIVLAKCEGSPQQRW 240

Query: 241 VAKEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGNPNQRWMF 285
           +AKEDGTILN  TNLVMDVKGSDPNLRQIILF  TGNPNQ+W++
Sbjct: 241 LAKEDGTILNLNTNLVMDVKGSDPNLRQIILFGRTGNPNQQWIW 284

[Close]

BLAST of Lag0001803 vs. ExPASy TrEMBL
Match: A0A5A7UV90 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G00550 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.2e-123
Identity = 204/282 (72.34%), Postives = 240/282 (85.11%), Query Frame = 0


Query: 3   KKMTAIVVCFVLAASLASIHGVSAAHKIILGRSTHIIGQDGLCLDVIGGYNDDHIPTQIW 62
           K  T I VC VLAASL  IH VSAA  I+L RSTH++GQDGLCLDVIGGY+D+H+PTQ+W
Sbjct: 9   KSTTIIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPTQLW 68

Query: 63  PCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSYTMINNCDTSAPDDKTWKLWLDGTLT 122
           PCGPQNNQLWTI+ DGT+R MGKCLVPNG+DPGSYTMI++C+ + P+DKTWKL+ DGTLT
Sbjct: 69  PCGPQNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDGTLT 128

Query: 123 HVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTAENGAPVVTAIVGIRRMCLQAQNDNV 182
           HV S LVLT+   G + ITTIE+NT A  QSW TA++G+P+V AIVG+RRMCLQAQNDN+
Sbjct: 129 HVRSSLVLTSQGTGAYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQNDNI 188

Query: 183 HVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVTGGGKGLNDAVVLGKCEGSAQQRWVA 242
           HVW+N CVK++R+QYWA + DGTIRANYNTS C++  GKG NDA+VL KCEG  QQRWVA
Sbjct: 189 HVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQRWVA 248

Query: 243 KEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGNPNQRWMF 285
           KEDGTIL+  TNLVMDVKGSDPNLR+IILF+ TGNPNQ WMF
Sbjct: 249 KEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMF 290

[Close]

BLAST of Lag0001803 vs. ExPASy TrEMBL
Match: A0A1S4E5V9 (nigrin b-like OS=Cucumis melo OX=3656 GN=LOC107992255 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.2e-123
Identity = 204/282 (72.34%), Postives = 240/282 (85.11%), Query Frame = 0


Query: 3   KKMTAIVVCFVLAASLASIHGVSAAHKIILGRSTHIIGQDGLCLDVIGGYNDDHIPTQIW 62
           K  T I VC VLAASL  IH VSAA  I+L RSTH++GQDGLCLDVIGGY+D+H+PTQ+W
Sbjct: 4   KSTTIIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPTQLW 63

Query: 63  PCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGSYTMINNCDTSAPDDKTWKLWLDGTLT 122
           PCGPQNNQLWTI+ DGT+R MGKCLVPNG+DPGSYTMI++C+ + P+DKTWKL+ DGTLT
Sbjct: 64  PCGPQNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDGTLT 123

Query: 123 HVHSGLVLTAHAAGPHPITTIESNTYATAQSWDTAENGAPVVTAIVGIRRMCLQAQNDNV 182
           HV S LVLT+   G + ITTIE+NT A  QSW TA++G+P+V AIVG+RRMCLQAQNDN+
Sbjct: 124 HVRSSLVLTSQGTGAYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQNDNI 183

Query: 183 HVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCVTGGGKGLNDAVVLGKCEGSAQQRWVA 242
           HVW+N CVK++R+QYWA + DGTIRANYNTS C++  GKG NDA+VL KCEG  QQRWVA
Sbjct: 184 HVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQRWVA 243

Query: 243 KEDGTILNPGTNLVMDVKGSDPNLRQIILFSATGNPNQRWMF 285
           KEDGTIL+  TNLVMDVKGSDPNLR+IILF+ TGNPNQ WMF
Sbjct: 244 KEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMF 285

[Close]

BLAST of Lag0001803 vs. ExPASy TrEMBL
Match: A0A6A4L4D0 (Uncharacterized protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3L33_15736 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.9e-71
Identity = 129/257 (50.19%), Postives = 169/257 (65.76%), Query Frame = 0


Query: 31  ILGRSTHIIGQDGLCLDVIGGYNDDHIPTQIWPCG-PQNNQLWTIRTDGTVRAMGKCLVP 90
           IL  + H+ G+DG C+DV  G ++D  P Q+WPCG  Q+NQ WT RTDGT+R++GKC+  
Sbjct: 89  ILNSTRHLSGRDGFCMDVRDGRDNDGNPIQLWPCGQQQSNQQWTFRTDGTIRSLGKCMTA 148

Query: 91  NGNDPGSYTMINNCDTSAPDDKTWKLWLDGTLTHVHSGLVLTA---HAAGPHPITTIESN 150
           +G  PG Y MI +C+T+  D   W L  DGT+T+ HSGLVLTA   H  G     T+E+N
Sbjct: 149 SGYSPGHYVMIFDCETAGQDATKWSLSTDGTITNPHSGLVLTATNEHVQG--NTLTVETN 208

Query: 151 TYATAQSWDTAENGAPVVTAIVGIRRMCLQAQNDNVHVWLNKCVKSDRKQYWAFYGDGTI 210
            +A +Q W   E   P VT I+G   +C+QA NDN  VWL  CV S ++Q WA YGDGTI
Sbjct: 209 IHAASQGWRVVEEVYPTVTTIMGFNDLCMQANNDNTRVWLESCVDSRQQQQWALYGDGTI 268

Query: 211 RANYNTSLCVTGGGKGLNDAVVLGKCEGSAQQRWVAKEDGTILNPGTNLVMDVKGSDPNL 270
           R N + +LC+T  G    D +++ +C+G   QRWV   DGTILNP   LVMDV+ SD +L
Sbjct: 269 RVNSDHNLCLTSDGHSSLDVIIVRRCQGWGNQRWVFHTDGTILNPNAKLVMDVRQSDVSL 328

Query: 271 RQIILFSATGNPNQRWM 284
           +QIIL+  TGNPNQRW+
Sbjct: 329 KQIILYQPTGNPNQRWL 343

[Close]

BLAST of Lag0001803 vs. ExPASy TrEMBL
Match: Q8GTA5 (Monomeric lectin SNAlm OS=Sambucus nigra OX=4202 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 8.0e-67
Identity = 116/247 (46.96%), Postives = 161/247 (65.18%), Query Frame = 0


Query: 37  HIIGQDGLCLDVIGGYNDDHIPTQIWPCGPQNNQLWTIRTDGTVRAMGKCLVPNGNDPGS 96
           +I+G+DGLC+DV  GY+ D  P Q+WPCG Q NQ WT  TD T+R+MGKC+  NG + GS
Sbjct: 64  NIVGRDGLCVDVRNGYDTDGTPIQLWPCGTQRNQQWTFHTDDTIRSMGKCMTANGLNNGS 123

Query: 97  YTMINNCDTSAPDDKTWKLWLDGTLTHVHSGLVLTAHAAGPHPITTIESNTYATAQSWDT 156
             MI NC T+  +   W++ +DG++ +  SGLV+TA +A    I  +++N YA +Q W  
Sbjct: 124 NIMIFNCSTAVENAIKWEVTIDGSIINPSSGLVITAPSAASRTILLLQNNIYAASQGWTV 183

Query: 157 AENGAPVVTAIVGIRRMCLQAQNDNVHVWLNKCVKSDRKQYWAFYGDGTIRANYNTSLCV 216
           + N  P+V +IVG R MCLQA  +N  VW+  C  +  +Q WA +GD TIR N N  LCV
Sbjct: 184 SNNVQPIVASIVGFREMCLQANGENNGVWMEDCEATSLQQQWALFGDRTIRVNSNRGLCV 243

Query: 217 TGGGKGLNDAVVLGKCEGSAQQRWVAKEDGTILNPGTNLVMDVKGSDPNLRQIILFSATG 276
           T  G    D +++ KC+G   QRW    DG I+NP + LVMDVK S+ +LR+II++ ATG
Sbjct: 244 TTNGYNSRDLIIILKCQGLPSQRWFFNSDGAIVNPKSKLVMDVKSSNVSLREIIIYPATG 303

Query: 277 NPNQRWM 284
            PNQ+W+
Sbjct: 304 RPNQQWV 310

The following BLAST results are available for this feature:

BLAST of Lag0001803 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150387.1	4.4e-128	75.00	nigrin b-like [Momordica charantia] >XP_022156716.1 nigrin b-like [Momordica cha...	[more]
XP_016903609.1	2.5e-123	72.34	PREDICTED: nigrin b-like [Cucumis melo]	[more]
KAA0059853.1	2.5e-123	72.34	nigrin b-like [Cucumis melo var. makuwa] >TYK24807.1 nigrin b-like [Cucumis melo...	[more]
XP_038885951.1	7.6e-120	70.57	nigrin b-like [Benincasa hispida]	[more]
KAG5543116.1	6.3e-74	53.20	hypothetical protein RHGRI_016012 [Rhododendron griersonianum]	[more]

BLAST of Lag0001803 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M653	2.4e-68	50.00	Ribosome-inactivating protein PMRIPt OS=Polygonatum multiflorum OX=45371 GN=RIPt...	[more]
P33183	3.5e-67	46.15	Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2	[more]
Q41358	1.2e-62	49.19	Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1	[more]
O22415	6.4e-61	48.78	Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=...	[more]
P93543	3.4e-54	45.75	Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1	[more]

BLAST of Lag0001803 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DVT2	2.2e-128	75.00	nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111023562 PE=3 SV=1	[more]
A0A5A7UV90	1.2e-123	72.34	Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G00550 ...	[more]
A0A1S4E5V9	1.2e-123	72.34	nigrin b-like OS=Cucumis melo OX=3656 GN=LOC107992255 PE=3 SV=1	[more]
A0A6A4L4D0	1.9e-71	50.19	Uncharacterized protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3...	[more]
Q8GTA5	8.0e-67	46.96	Monomeric lectin SNAlm OS=Sambucus nigra OX=4202 PE=2 SV=1	[more]

BLAST of Lag0001803 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000772	Ricin B, lectin domain	SMART	SM00458	ricin_3	coord: 33..157 e-value: 2.1E-17 score: 73.8 coord: 161..285 e-value: 2.1E-25 score: 100.4
IPR000772	Ricin B, lectin domain	PFAM	PF00652	Ricin_B_lectin	coord: 167..282 e-value: 1.5E-22 score: 80.2 coord: 36..154 e-value: 1.2E-16 score: 61.1
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 160..285 score: 33.265499
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 34..157 score: 26.533501
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 167..284 e-value: 3.65947E-25 score: 95.265
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 37..156 e-value: 6.41321E-16 score: 70.6122
None	No IPR available	GENE3D	2.80.10.50		coord: 162..284 e-value: 1.3E-43 score: 149.6
None	No IPR available	GENE3D	2.80.10.50		coord: 32..161 e-value: 1.2E-30 score: 108.2
None	No IPR available	PANTHER	PTHR33453:SF23	RRNA N-GLYCOSIDASE	coord: 37..283
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 37..283
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 35..156
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 162..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0001803.1	Lag0001803.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity