Lag0001800 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0001800
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAuxin-responsive GH3 family protein
Locationchr4: 35499661 .. 35503518 (-)
RNA-Seq ExpressionLag0001800
SyntenyLag0001800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAGAGAAGATGGAGGAGTTTGATATGGAGAAGGTGATTGAGGAATTTGAGGAATTGACTAAAGATGCTGAGAGAGTTCAAAGAGAGACTTTGAAAATGATTTTGGAAGAAAATGGATCTGCTGAGTATTTGCAGAATTTGGGGCTTAATGGAAAAACAGATCCCGTGAGTTTCAAGGCTTGTGTTCCCATTGTAACACATGAGGACTTGGAGCCTTATATTCAGAGAATTGCTGATGGTCCTTGTGCTCCGATTCTTACCGGAAAACCGATCACTACGATCTCGTTAAGGTAGTTTAGTCTGGTTGCTTTAGTTTTATACCTTGTTTGAATATCCTTGAATGGAAGATTGAGATTGTTTTGGTGTATGTTGTAGTTCTGGTACTACTCAGGGGAAACCGAAGTTCGTCCCATTCAATGATGAGTTGATTGAAACTACAATACAGATATTTCGGACTTCTTTCGCGTTTAGAAACCGGTACGAAACAATTGGTACTTTTACAGTTTGGAATATGTTGAGTGATTTAATCATGTTGTGTGATTTGTTGAAGAGGATTCATCTGTTTGTGCAGAGATTTTCCAATTGAGAAAGGGAGGTCCTTGATGTTTATTTATGGCAGCAAGCAGTTCAAAACCAATGGAGATTTAATGGCTGCTACTGCCACGAGTAACCTGTACCGCGATCCGAGATTTAAGAGCACAATGAAAGCAATCATGTCTACGACCTGCAGCCCAGATGAAGTCATGTTTGGTTCTGATTTCCATCAATCACTGTATTGTCATCTGCTGTGTGGGCTTATTTTTAGGGATGAAATTCAGATGATATTCTCGACGTTTGCTCATAGCATCGTTCACGCGTTTAGAACGTTTGAAAGCATCTGGGAAGAGCTATGTTGTGACATTCAAAGTGGTGTTCTCTCCAGCCGAATCACGTCGCCTTCCATTCGTGCAGGGATGTTGAAATTGCTGAAACCGAATCCCGAACTGGCAAATTTGGTGTACGAAAAGTGTGTTGGATTGAGTAATTGGTATGGATTGATACCCCAACTCTTCCCTAACGCAAAGTACATATATGGAATCATGACCGGATCGATGGAACCATATGTGAAAAAGTTGAGACACTATGCAGGAGAGTTGCCTTTGATGAGTGCTGATTATGGTTCATCCGAAGGATGGATTGGTGCAAATACAAACCCGAAAATGCCTCCCGAATTCGCTACTTTCACCGTGCTTCCCAATATTGGTTACTTCGAATTCATTCCTCTCGGGGACGACATCTTCGAGCATGATAAGACTCAATCTTCTTCAATCTGCAACGAGTTGAAACCGGTGGATCTTACTCGAGTCCAAGTTGGTAAAGAGTATGAGATTCTAATCACAAGTGTTGCAGGTACTTCTGTCTTGATTGTTGGCCAATTCATTATACTTTTGTTGTTATTTTACCTCACTTTCAAGATGTCCCATAACTAGTTTTAGTAACCAAAAGAGAAGGATTCCAGGTCTGTATAGGTATAGGCTTGGAGATGTGGTTAAGGTCACAGGCTTCCATAACTCGACCCCGGTACTCAAATTCGTCTGTCGGAGAAACCTCCTGCTAACGATCAACATCGACAAGAACACTGAGAAAGACTTGCAGATAGCTGTTGAGGAAGCCTCAAAGCTACTAGCAGAGGAGAAACTCGAAGTTGTCGATTTCACGAGCTACGTGGATCGATCTACAGAGCCTGGCCACTACGTCATCTTCTGGGAGATGAGTGGTGAAGCTTATAACGACGACATTTTGAAGCAATGCTGCAACTGTTTGGATAGTTCCTTTGTTGATGCAGGCTATGTTAGCTCACGTAGAGTCAAGGCAATTGGCGCGTTGGAACTTCGGGTGGTCCAGAGAGGAACATTTCAGAAGATTCTAGATCACTTCCTGTCGTTAGGGGCGGCGGTGAGTCAGTTTAAAACGCCTCGATGCATCGGTCCAGCCAACAATAAGGTGCTGCAGATCTTGTGCAGTAATGTCAACAAGGTCTACTTCAGTACTGCTTTTCAGTAAGAGCAGCAAGAGAGCTGGAAAAAGAAATGCTATCCTTTTCCCTCAAAACAGTCAAAACATTAATCAAACTGTAAAGTTACAAAGAGAAAAAAAGGTTAATTTTTATAGTTTCAATGATGCCCTCAAATATATAAATTGTTTTAAAGATGTCCTTAAGTTTGTTTAGGAGAAATGATTCTTCATTTCTTTTTTCCAAACAGAATTAGGACTACAAATATTTTAAAAATAATTGATAAATTTGAGGGCATTTTTGAAATGTTAAAACTTTAAGATTAAACTGTACTTCAAGGTAAAAAAGTAAGGTTTAGGTAGAAAGAAGGGAAAGATTGTAAAAAAAAAAAAAAGTGTTGCAGTTAGATTCAATTCAGGCTGTAAATGAATGTATTCAGATGTCAAATCCCTTTGTGACAAATGAAAAAGTTAAAATATGCCATTAGCTCTTTGTACTTTAACTTTTATAATGCATTGGTTCTCATCTCATATACATGAATATAATGATTTTGTCTTTTACCCCCGAACTTGAAACAAATGCAACATTAAATCATGGTTAAATACGTAGGGACAGTTGCATAGATGACAAATTGGACCAAATTATTAGCAAATATAGCACAGTTGCAAACTAATTGCATATATAGCACAATTAATTGTACAAGACCAAAAAGCTATCGTTAGATTCTATCCGTGTCTATCACTGATAGACTTCTATTATTCAAAATCTATCGTTGATAGACTCCGATTAATCAAAATCATCAAAATCTCGGAGTCTATCAGTGATAGTCACAGATAGATTTTGATTAATCGGAGTCTATAAGCGATAGATACGGATAGATTCTAAATAATTGGAGTCTATCAGTAGACTGATAGATTTTGATGAATCAGTGTCTATTGCTGATAAACTCTAATAATCGAAATCTATTAGATAGATTCTAATTAACTAGAGTCTATTAGTGTCTATCAGTAATATACTATGATAGGTTGTGCTATATTTGTTATTCTTTGGAAAAAATACACTTTTGGTCCCTGAGATTTGGGTTAAGCGACACTTTGGTCCCTGAGTTTTCAATCTCAACAAAAATACCCCTGAGGTTTGAGATAAGTAACACTTTGGTCCCTGCCATCTATTTTGCCGTTAAACCCTAACGGTAAGCTGACGTGACATTATTATTTTAATTAAAAAAATGAATTTTTGATTTTTTAAATAAAAATATATAAAAATATTTAAAATATTATTATTTTATTATTATTTTTTAAATTTTCTCTCTTTCTTCTTCATCTTCCTTCTCATCTTCTTCATTCCCTCTCCCATCTTCTTCCTTCCCCTCCCATCTTCTTCAGTTCCGGTGGCCGGCCGCCGCCGGCTGTCGGCGAACGGTGGCGGTCGCCGGATTTTTTTTTTTTTTCTTCTCTTCTTCTCTTCTTCCCTTCTTCTTCTTCTTTCTCTTACTTTTTCTGTTTTTTTTTTTTTAAGGGAATTTCGGGGGAGGAGGAGCTCTCCTCCCTGATTCCGGAGCCGAATCGGGGAGGAGAGCTCCTCCTCCCCTTCCCGTTTCAGATCTGAAACGGGAAGGGGAGGAGGAGCTCCTCCTCCTCCGAAATTCCCTTAAAAAAAACAGAAAAAGTAAGAGAAAGAAGAAGGGAAGAAGAGAAGAAGGAGAAAAAAAAAATTTCGGCGACCGCCGCCGTTCGCCGACAGCCGGCGACGGCCGGCCACCAGAACTAAAGAAGATGGGAGGGGGAAGGAAGAAGATGGGAGAGGGAAGGAAGAAGATGAGAAGGAAGATGAAGAAGAAAGAGAGAAAATTTAAAAAATAA

mRNA sequence

ATGTTAGAGAAGATGGAGGAGTTTGATATGGAGAAGGTGATTGAGGAATTTGAGGAATTGACTAAAGATGCTGAGAGAGTTCAAAGAGAGACTTTGAAAATGATTTTGGAAGAAAATGGATCTGCTGAGTATTTGCAGAATTTGGGGCTTAATGGAAAAACAGATCCCGTGAGTTTCAAGGCTTGTGTTCCCATTGTAACACATGAGGACTTGGAGCCTTATATTCAGAGAATTGCTGATGGTCCTTGTGCTCCGATTCTTACCGGAAAACCGATCACTACGATCTCGTTAAGTTCTGGTACTACTCAGGGGAAACCGAAGTTCGTCCCATTCAATGATGAGTTGATTGAAACTACAATACAGATATTTCGGACTTCTTTCGCGTTTAGAAACCGAGATTTTCCAATTGAGAAAGGGAGGTCCTTGATGTTTATTTATGGCAGCAAGCAGTTCAAAACCAATGGAGATTTAATGGCTGCTACTGCCACGAGTAACCTGTACCGCGATCCGAGATTTAAGAGCACAATGAAAGCAATCATGTCTACGACCTGCAGCCCAGATGAAGTCATGTTTGGTTCTGATTTCCATCAATCACTGTATTGTCATCTGCTGTGTGGGCTTATTTTTAGGGATGAAATTCAGATGATATTCTCGACGTTTGCTCATAGCATCGTTCACGCGTTTAGAACGTTTGAAAGCATCTGGGAAGAGCTATGTTGTGACATTCAAAGTGGTGTTCTCTCCAGCCGAATCACGTCGCCTTCCATTCGTGCAGGGATGTTGAAATTGCTGAAACCGAATCCCGAACTGGCAAATTTGGTGTACGAAAAGTGTGTTGGATTGAGTAATTGGTATGGATTGATACCCCAACTCTTCCCTAACGCAAAGTACATATATGGAATCATGACCGGATCGATGGAACCATATGTGAAAAAGTTGAGACACTATGCAGGAGAGTTGCCTTTGATGAGTGCTGATTATGGTTCATCCGAAGGATGGATTGGTGCAAATACAAACCCGAAAATGCCTCCCGAATTCGCTACTTTCACCGTGCTTCCCAATATTGGTTACTTCGAATTCATTCCTCTCGGGGACGACATCTTCGAGCATGATAAGACTCAATCTTCTTCAATCTGCAACGAGTTGAAACCGGTGGATCTTACTCGAGTCCAAGTTGGTAAAGAGTATGAGATTCTAATCACAAGTGTTGCAGGTCTGTATAGGTATAGGCTTGGAGATGTGGTTAAGGTCACAGGCTTCCATAACTCGACCCCGGTACTCAAATTCGTCTGTCGGAGAAACCTCCTGCTAACGATCAACATCGACAAGAACACTGAGAAAGACTTGCAGATAGCTGTTGAGGAAGCCTCAAAGCTACTAGCAGAGGAGAAACTCGAAGTTGTCGATTTCACGAGCTACGTGGATCGATCTACAGAGCCTGGCCACTACGTCATCTTCTGGGAGATGAGTGGTGAAGCTTATAACGACGACATTTTGAAGCAATGCTGCAACTGTTTGGATAGTTCCTTTGTTGATGCAGGCTATGTTAGCTCACGTAGAGTCAAGGCAATTGGCGCGTTGGAACTTCGGGTGGTCCAGAGAGGAACATTTCAGAAGATTCTAGATCACTTCCTGTCGTTAGGGGCGGCGGTGAGTCAGTTTAAAACGCCTCGATGCATCGGTCCAGCCAACAATAAGGTGCTGCAGATCTTGTGCAGTAATGTCAACAAGGTCTACTTCAGGAATTTCGGGGGAGGAGGAGCTCTCCTCCCTGATTCCGGAGCCGAATCGGGGAGGAGAGCTCCTCCTCCCCTTCCCGTTTCAGATCTGAAACGGGAAGGGGAGGAGGAGCTCCTCCTCCTCCGAAATTCCCTTAAAAAAAACAGAAAAAGTAAGAGAAAGAAGAAGGGAAGAAGAGAAGAAGGAGAAAAAAAAAATTTCGGCGACCGCCGCCGTTCGCCGACAGCCGGCGACGGCCGGCCACCAGAACTAAAGAAGATGGGAGGGGGAAGGAAGAAGATGGGAGAGGGAAGGAAGAAGATGAGAAGGAAGATGAAGAAGAAAGAGAGAAAATTTAAAAAATAA

Coding sequence (CDS)

ATGTTAGAGAAGATGGAGGAGTTTGATATGGAGAAGGTGATTGAGGAATTTGAGGAATTGACTAAAGATGCTGAGAGAGTTCAAAGAGAGACTTTGAAAATGATTTTGGAAGAAAATGGATCTGCTGAGTATTTGCAGAATTTGGGGCTTAATGGAAAAACAGATCCCGTGAGTTTCAAGGCTTGTGTTCCCATTGTAACACATGAGGACTTGGAGCCTTATATTCAGAGAATTGCTGATGGTCCTTGTGCTCCGATTCTTACCGGAAAACCGATCACTACGATCTCGTTAAGTTCTGGTACTACTCAGGGGAAACCGAAGTTCGTCCCATTCAATGATGAGTTGATTGAAACTACAATACAGATATTTCGGACTTCTTTCGCGTTTAGAAACCGAGATTTTCCAATTGAGAAAGGGAGGTCCTTGATGTTTATTTATGGCAGCAAGCAGTTCAAAACCAATGGAGATTTAATGGCTGCTACTGCCACGAGTAACCTGTACCGCGATCCGAGATTTAAGAGCACAATGAAAGCAATCATGTCTACGACCTGCAGCCCAGATGAAGTCATGTTTGGTTCTGATTTCCATCAATCACTGTATTGTCATCTGCTGTGTGGGCTTATTTTTAGGGATGAAATTCAGATGATATTCTCGACGTTTGCTCATAGCATCGTTCACGCGTTTAGAACGTTTGAAAGCATCTGGGAAGAGCTATGTTGTGACATTCAAAGTGGTGTTCTCTCCAGCCGAATCACGTCGCCTTCCATTCGTGCAGGGATGTTGAAATTGCTGAAACCGAATCCCGAACTGGCAAATTTGGTGTACGAAAAGTGTGTTGGATTGAGTAATTGGTATGGATTGATACCCCAACTCTTCCCTAACGCAAAGTACATATATGGAATCATGACCGGATCGATGGAACCATATGTGAAAAAGTTGAGACACTATGCAGGAGAGTTGCCTTTGATGAGTGCTGATTATGGTTCATCCGAAGGATGGATTGGTGCAAATACAAACCCGAAAATGCCTCCCGAATTCGCTACTTTCACCGTGCTTCCCAATATTGGTTACTTCGAATTCATTCCTCTCGGGGACGACATCTTCGAGCATGATAAGACTCAATCTTCTTCAATCTGCAACGAGTTGAAACCGGTGGATCTTACTCGAGTCCAAGTTGGTAAAGAGTATGAGATTCTAATCACAAGTGTTGCAGGTCTGTATAGGTATAGGCTTGGAGATGTGGTTAAGGTCACAGGCTTCCATAACTCGACCCCGGTACTCAAATTCGTCTGTCGGAGAAACCTCCTGCTAACGATCAACATCGACAAGAACACTGAGAAAGACTTGCAGATAGCTGTTGAGGAAGCCTCAAAGCTACTAGCAGAGGAGAAACTCGAAGTTGTCGATTTCACGAGCTACGTGGATCGATCTACAGAGCCTGGCCACTACGTCATCTTCTGGGAGATGAGTGGTGAAGCTTATAACGACGACATTTTGAAGCAATGCTGCAACTGTTTGGATAGTTCCTTTGTTGATGCAGGCTATGTTAGCTCACGTAGAGTCAAGGCAATTGGCGCGTTGGAACTTCGGGTGGTCCAGAGAGGAACATTTCAGAAGATTCTAGATCACTTCCTGTCGTTAGGGGCGGCGGTGAGTCAGTTTAAAACGCCTCGATGCATCGGTCCAGCCAACAATAAGGTGCTGCAGATCTTGTGCAGTAATGTCAACAAGGTCTACTTCAGGAATTTCGGGGGAGGAGGAGCTCTCCTCCCTGATTCCGGAGCCGAATCGGGGAGGAGAGCTCCTCCTCCCCTTCCCGTTTCAGATCTGAAACGGGAAGGGGAGGAGGAGCTCCTCCTCCTCCGAAATTCCCTTAAAAAAAACAGAAAAAGTAAGAGAAAGAAGAAGGGAAGAAGAGAAGAAGGAGAAAAAAAAAATTTCGGCGACCGCCGCCGTTCGCCGACAGCCGGCGACGGCCGGCCACCAGAACTAAAGAAGATGGGAGGGGGAAGGAAGAAGATGGGAGAGGGAAGGAAGAAGATGAGAAGGAAGATGAAGAAGAAAGAGAGAAAATTTAAAAAATAA

Protein sequence

MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTIQIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCCDIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEFIPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVTGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFRNFGGGGALLPDSGAESGRRAPPPLPVSDLKREGEEELLLLRNSLKKNRKSKRKKKGRREEGEKKNFGDRRRSPTAGDGRPPELKKMGGGRKKMGEGRKKMRRKMKKKERKFKK
Homology
BLAST of Lag0001800 vs. NCBI nr
Match: XP_022158613.1 (jasmonic acid-amido synthetase JAR1-like isoform X1 [Momordica charantia])

HSP 1 Score: 1047.3 bits (2707), Expect = 5.6e-302
Identity = 515/580 (88.79%), Postives = 549/580 (94.66%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEE E ++K+A RVQRETLK ILEENGS+EYLQNLGLNG TDP SFK
Sbjct: 1   MLEKMEEFDTEKVIEEVEAMSKEAGRVQRETLKRILEENGSSEYLQNLGLNGNTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
           ACVPIVTHEDLEPYIQRIAD   A ILT KPITTISLSSGTT+GKPKFVPFNDEL+++TI
Sbjct: 61  ACVPIVTHEDLEPYIQRIADSHHASILTAKPITTISLSSGTTEGKPKFVPFNDELVDSTI 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
           QIF+ SFAFRNRDFPI KGRSLMFIYGSKQFKTNG L AATATSNLYRDP+FKSTMK+IM
Sbjct: 121 QIFQASFAFRNRDFPIGKGRSLMFIYGSKQFKTNGGLTAATATSNLYRDPKFKSTMKSIM 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +CSPDEV++GSDFHQSLYCHLLCGLIFRDEIQ+IFSTFAHSIVHAFRTFE IWEELCC
Sbjct: 181 SMSCSPDEVIYGSDFHQSLYCHLLCGLIFRDEIQLIFSTFAHSIVHAFRTFEHIWEELCC 240

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI++G+L+SRITSPSIRAGM  LLKPNPELA+LVYEKCVGLS WYGLIPQLFPNAKYIYG
Sbjct: 241 DIRNGILTSRITSPSIRAGMSNLLKPNPELADLVYEKCVGLSTWYGLIPQLFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPYVKKLRHYAG+LPLMSADYGSSEGWIGAN NPK+ PEFATFTVLPNIGYFEF
Sbjct: 301 IMTGSMEPYVKKLRHYAGDLPLMSADYGSSEGWIGANINPKLSPEFATFTVLPNIGYFEF 360

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPLG +I E+DK+QSS IC+ELKPVDLT+VQVGKEYEILITSVAG+YRYRLGDVVKVTGF
Sbjct: 361 IPLG-EISENDKSQSSLICSELKPVDLTQVQVGKEYEILITSVAGMYRYRLGDVVKVTGF 420

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           +NSTP LKFVCRRNLLLTINIDKNTEKDLQIAV EASKLLAEEKLEVVDFTSYVDRST+P
Sbjct: 421 YNSTPELKFVCRRNLLLTINIDKNTEKDLQIAVGEASKLLAEEKLEVVDFTSYVDRSTDP 480

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWEMSGEAYNDD+L +CCN LD+SFVDAGYVSSRRVK+IGALELRVVQRGTFQKI
Sbjct: 481 GHYVIFWEMSGEAYNDDLLNRCCNRLDTSFVDAGYVSSRRVKSIGALELRVVQRGTFQKI 540

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNV KVYF
Sbjct: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVIKVYF 579

BLAST of Lag0001800 vs. NCBI nr
Match: XP_022158615.1 (jasmonic acid-amido synthetase JAR1-like isoform X2 [Momordica charantia])

HSP 1 Score: 969.5 bits (2505), Expect = 1.5e-278
Identity = 487/580 (83.97%), Postives = 516/580 (88.97%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEE E ++K+A RVQRETLK ILEENGS+EYLQNLGLNG TDP SFK
Sbjct: 1   MLEKMEEFDTEKVIEEVEAMSKEAGRVQRETLKRILEENGSSEYLQNLGLNGNTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
           ACVPIVTHEDLEPYIQRIAD   A ILT KPITTISL                       
Sbjct: 61  ACVPIVTHEDLEPYIQRIADSHHASILTAKPITTISL----------------------- 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
                      RDFPI KGRSLMFIYGSKQFKTNG L AATATSNLYRDP+FKSTMK+IM
Sbjct: 121 -----------RDFPIGKGRSLMFIYGSKQFKTNGGLTAATATSNLYRDPKFKSTMKSIM 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +CSPDEV++GSDFHQSLYCHLLCGLIFRDEIQ+IFSTFAHSIVHAFRTFE IWEELCC
Sbjct: 181 SMSCSPDEVIYGSDFHQSLYCHLLCGLIFRDEIQLIFSTFAHSIVHAFRTFEHIWEELCC 240

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI++G+L+SRITSPSIRAGM  LLKPNPELA+LVYEKCVGLS WYGLIPQLFPNAKYIYG
Sbjct: 241 DIRNGILTSRITSPSIRAGMSNLLKPNPELADLVYEKCVGLSTWYGLIPQLFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPYVKKLRHYAG+LPLMSADYGSSEGWIGAN NPK+ PEFATFTVLPNIGYFEF
Sbjct: 301 IMTGSMEPYVKKLRHYAGDLPLMSADYGSSEGWIGANINPKLSPEFATFTVLPNIGYFEF 360

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPLG +I E+DK+QSS IC+ELKPVDLT+VQVGKEYEILITSVAG+YRYRLGDVVKVTGF
Sbjct: 361 IPLG-EISENDKSQSSLICSELKPVDLTQVQVGKEYEILITSVAGMYRYRLGDVVKVTGF 420

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           +NSTP LKFVCRRNLLLTINIDKNTEKDLQIAV EASKLLAEEKLEVVDFTSYVDRST+P
Sbjct: 421 YNSTPELKFVCRRNLLLTINIDKNTEKDLQIAVGEASKLLAEEKLEVVDFTSYVDRSTDP 480

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWEMSGEAYNDD+L +CCN LD+SFVDAGYVSSRRVK+IGALELRVVQRGTFQKI
Sbjct: 481 GHYVIFWEMSGEAYNDDLLNRCCNRLDTSFVDAGYVSSRRVKSIGALELRVVQRGTFQKI 540

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNV KVYF
Sbjct: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVIKVYF 545

BLAST of Lag0001800 vs. NCBI nr
Match: XP_041001957.1 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 [Juglans microcarpa x Juglans regia] >XP_041001958.1 jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 941.8 bits (2433), Expect = 3.3e-270
Identity = 453/582 (77.84%), Postives = 518/582 (89.00%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 1   MLEKMEEFDPEKVIEEFEAITQDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTHE+LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 61  TCVPLVTHEELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G++L FIY SKQF+T G L A TAT+N+YR+ +FK TMKAI 
Sbjct: 121 QIYRTSFAFRNREFPIGNGKALQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 181 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIIHAFRTFEQVWEELCI 240

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLSSR+++PSIRA M KLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 241 DIREGVLSSRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+PPE ATF VLPNIG+FEF
Sbjct: 301 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPELATFAVLPNIGFFEF 360

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT V++G++YEI++T+ AGLYRYRLGDVVKV 
Sbjct: 361 IPLKHNIEDQDQNQMDPSFFQIEPEPVDLTEVRIGEDYEIIVTNFAGLYRYRLGDVVKVM 420

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 421 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 480

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SFVDAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 481 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFVDAGYVSSRKVNAIGALELRVVRRGTFQ 540

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           KILDH+L LG+AVSQFKTPRC+GP NN VLQILC NV + YF
Sbjct: 541 KILDHYLGLGSAVSQFKTPRCVGPTNNLVLQILCDNVVESYF 581

BLAST of Lag0001800 vs. NCBI nr
Match: XP_041001955.1 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 [Juglans microcarpa x Juglans regia] >XP_041001956.1 jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 941.8 bits (2433), Expect = 3.3e-270
Identity = 453/582 (77.84%), Postives = 518/582 (89.00%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 22  MLEKMEEFDPEKVIEEFEAITQDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 81

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTHE+LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 82  TCVPLVTHEELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 141

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G++L FIY SKQF+T G L A TAT+N+YR+ +FK TMKAI 
Sbjct: 142 QIYRTSFAFRNREFPIGNGKALQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 201

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 202 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIIHAFRTFEQVWEELCI 261

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLSSR+++PSIRA M KLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 262 DIREGVLSSRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 321

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+PPE ATF VLPNIG+FEF
Sbjct: 322 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPELATFAVLPNIGFFEF 381

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT V++G++YEI++T+ AGLYRYRLGDVVKV 
Sbjct: 382 IPLKHNIEDQDQNQMDPSFFQIEPEPVDLTEVRIGEDYEIIVTNFAGLYRYRLGDVVKVM 441

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 442 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 501

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SFVDAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 502 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFVDAGYVSSRKVNAIGALELRVVRRGTFQ 561

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           KILDH+L LG+AVSQFKTPRC+GP NN VLQILC NV + YF
Sbjct: 562 KILDHYLGLGSAVSQFKTPRCVGPTNNLVLQILCDNVVESYF 602

BLAST of Lag0001800 vs. NCBI nr
Match: XP_041001954.1 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X1 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 941.8 bits (2433), Expect = 3.3e-270
Identity = 453/582 (77.84%), Postives = 518/582 (89.00%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 46  MLEKMEEFDPEKVIEEFEAITQDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 105

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTHE+LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 106 TCVPLVTHEELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 165

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G++L FIY SKQF+T G L A TAT+N+YR+ +FK TMKAI 
Sbjct: 166 QIYRTSFAFRNREFPIGNGKALQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 225

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 226 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIIHAFRTFEQVWEELCI 285

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLSSR+++PSIRA M KLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 286 DIREGVLSSRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 345

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+PPE ATF VLPNIG+FEF
Sbjct: 346 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPELATFAVLPNIGFFEF 405

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT V++G++YEI++T+ AGLYRYRLGDVVKV 
Sbjct: 406 IPLKHNIEDQDQNQMDPSFFQIEPEPVDLTEVRIGEDYEIIVTNFAGLYRYRLGDVVKVM 465

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 466 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 525

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SFVDAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 526 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFVDAGYVSSRKVNAIGALELRVVRRGTFQ 585

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           KILDH+L LG+AVSQFKTPRC+GP NN VLQILC NV + YF
Sbjct: 586 KILDHYLGLGSAVSQFKTPRCVGPTNNLVLQILCDNVVESYF 626

BLAST of Lag0001800 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 870.5 bits (2248), Expect = 1.2e-251
Identity = 418/580 (72.07%), Postives = 489/580 (84.31%), Query Frame = 0

Query: 1   MLEKME-EFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSF 60
           ++EK+E +FD EKVIEEFE+LTKDA ++Q ETLK ILE+NG  EYLQ  GLNG+TDP +F
Sbjct: 4   LVEKIEKKFDQEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLWGLNGRTDPQTF 63

Query: 61  KACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETT 120
           K CVPIVTH DLEPYIQRIADG  +PILTGKPI TISLSSGTTQGKPKFVPFNDEL+E+T
Sbjct: 64  KNCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMEST 123

Query: 121 IQIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAI 180
           +QIF+TSFAFRNR+FPI  G++L FIY SKQFKT G L A TAT+N+YR+ +FK TMKA+
Sbjct: 124 MQIFKTSFAFRNREFPIGNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAM 183

Query: 181 MSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELC 240
            +  CSPDEV+FG DFHQSLYCHLLCGLIF DE+Q++ STFAHSIVHAFRTFE +WE L 
Sbjct: 184 CTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALV 243

Query: 241 CDIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIY 300
            DI+ GVLSSR+T PSIR  M KLLKP+PELA+ +Y KC  LSNWYGLIP LFPN +YIY
Sbjct: 244 VDIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRLSNWYGLIPDLFPNTRYIY 303

Query: 301 GIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFE 360
           GIMTGSMEPY+KKLRHYAGELPL+SADYGSSEGW+G N NPK+PPE  T+ VLPNIGYFE
Sbjct: 304 GIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGYFE 363

Query: 361 FIPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVTG 420
           FIPLG ++   ++  S        PV LT V++G+EYE++ T+ AGLYRYRLGDVVKV G
Sbjct: 364 FIPLGGNLNGIEQANS--------PVGLTEVKLGEEYEVVFTNFAGLYRYRLGDVVKVKG 423

Query: 421 FHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTE 480
           FHN TP L+FVCR NLLL+INIDKNTEKDLQ+AVE A+K L +EKLEVVDFTS+V+ S +
Sbjct: 424 FHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEKLEVVDFTSHVNVSAD 483

Query: 481 PGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQK 540
           PGHYVIFWE+SGEA  D++L+ CCNCLD SF+DAGYVSSR+V AIGALELR+V+RGTF K
Sbjct: 484 PGHYVIFWELSGEA-TDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALELRIVKRGTFHK 543

Query: 541 ILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVY 580
           ILDHF+ LG AVSQFKTPRC+GP N+ +LQIL SNV + Y
Sbjct: 544 ILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETY 574

BLAST of Lag0001800 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 1.8e-250
Identity = 414/580 (71.38%), Postives = 494/580 (85.17%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           ++EK E+FD E+VIEEFE LTKDA ++Q ETL+ ILEENG  EYLQ  GLNGKTD +SFK
Sbjct: 4   VVEKTEKFDPEEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQWGLNGKTDSLSFK 63

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            C+PIVTH+DLEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFN+EL+E+T+
Sbjct: 64  NCIPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTM 123

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
           QIF+TSF FRNR+FP+  G++L FIYGSKQFKT G L A TAT+N+YR+ +FK TMKA+ 
Sbjct: 124 QIFKTSFVFRNREFPVVNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMQ 183

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           +  CSPDEV+FG DF QSLYCHLLCGLIFRDE+Q++ STFAHSIVHAFR FE IW+EL  
Sbjct: 184 TPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELVT 243

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           +I+ GVLSSR+  PS+RA M KLLKP+PELA+ ++ KC  LSNWYGLIP+LFPN +YIYG
Sbjct: 244 NIREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRLSNWYGLIPELFPNTRYIYG 303

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPY+KKLRHYAG+LPL+SADYGSSEGWIGAN NP++PPE  T+ VLPNIGYFEF
Sbjct: 304 IMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGYFEF 363

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPL +++             E  PV LT V++G+EYEI++T+ AGLYRYRLGDVVK+ GF
Sbjct: 364 IPLMENLDG----------LEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGDVVKIKGF 423

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           HN TP L+F+CRRNLLL+INIDKNTEKDLQ+AVE A+K+L++EKLEVVDFTS+V+ S +P
Sbjct: 424 HNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEKLEVVDFTSHVNVSADP 483

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWE++GEA +++ILK+CCNCLD SFVDAGYV SR+V AIGALELR+V+RGTF KI
Sbjct: 484 GHYVIFWELNGEA-SEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVKRGTFHKI 543

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           LDHF+ LGAAVSQFKTPRC+GP N  VLQIL SNV + YF
Sbjct: 544 LDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYF 572

BLAST of Lag0001800 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 4.3e-236
Identity = 376/570 (65.96%), Postives = 474/570 (83.16%), Query Frame = 0

Query: 11  EKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFKACVPIVTHED 70
           E+ I EFE LT+DA RVQ++TLK ILE N SAEYLQN GL G+TD  S+K+C+P+  H D
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAESYKSCIPLCVHND 66

Query: 71  LEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTIQIFRTSFAFR 130
           +EPYIQRI DG  +P++TG+PIT +SLSSGTT GKPKF+PFNDEL+ETT+QI+RTS+AFR
Sbjct: 67  IEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQIYRTSYAFR 126

Query: 131 NRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIMSTTCSPDEVM 190
           NR++PI +G++L F+YGSKQ  T G ++A TAT+NLYR  R+K  MK I S  CSPDEV+
Sbjct: 127 NREYPIGQGKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQSQCCSPDEVI 186

Query: 191 FGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCCDIQSGVLSSR 250
           FG DFHQSLYCHLLCGLI+ +E+  +FSTFAHS+VHAF+TFE +WE+LC DI+ GVLS +
Sbjct: 187 FGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCTDIRDGVLSKK 246

Query: 251 ITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYGIMTGSMEPYV 310
           +T+PSIR  + K+LKPNPELA+ +Y+KC+GLSNWYG+IP L+PNAKY+YGIMTGSMEPY+
Sbjct: 247 VTAPSIREAVSKILKPNPELADSIYKKCIGLSNWYGVIPALWPNAKYVYGIMTGSMEPYL 306

Query: 311 KKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEFIPLGDDIFEH 370
           KKLRHYAG LPL+SADYG+SEGW+G+N +P +PPE  T+ VLP +GYFEFIPL   I E 
Sbjct: 307 KKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEE 366

Query: 371 DKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVTGFHNSTPVLKFV 430
            +  +S    E  PV LT V+VGK YE++IT+ AGLYRYRLGDVVK+  FHNSTP L+F+
Sbjct: 367 TENSASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDVVKIARFHNSTPELQFI 426

Query: 431 CRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEPGHYVIFWEMS 490
           CRR+L+L+INIDKNTEKDLQ+AVEEASK L  EKLEV+DFTS+V+RS++PG YVIFWE+S
Sbjct: 427 CRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELS 486

Query: 491 GEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKILDHFLSLGAA 550
           G+A +D++L  C N LD +F+DAGY  SR++K IG LELR++++GTF++ILDHFLSLG A
Sbjct: 487 GDA-SDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGA 546

Query: 551 VSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           VSQFKTPR + P+N+KVLQIL  NV + YF
Sbjct: 547 VSQFKTPRFVNPSNSKVLQILSRNVTQSYF 575

BLAST of Lag0001800 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 789.6 bits (2038), Expect = 2.8e-227
Identity = 373/583 (63.98%), Postives = 474/583 (81.30%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGK-TDP-VS 60
           MLEK+E FDM +VI+EF+E+T++A +VQ++TLK IL +N SA YLQN GLNG  TDP  +
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIET 120
           FK+ VP+VT  +LEPYI+R+ DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TIQIFRTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMK 180
           T+Q+FRT+FAFRNRDFPI + G++L FI+ SKQ+ + G +   TAT+N+YR+P FK+ MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEE 240
           +I S +CSPDEV+F  D HQ+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCCDIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKY 300
           +  DI+ GVLS+RIT PS+R  M KLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGY 360
           +YGIMTGSMEPYV KLRHYAG+LPL+S DYGSSEGWI AN  P++ PE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKV 420
           FEF+P+           S +   E KPV LT+V++G+EYE++IT+ AGLYRYRLGDVVKV
Sbjct: 361 FEFLPV-----------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKV 420

Query: 421 TGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRS 480
            GF+N+TP LKF+CRRNL+L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D S
Sbjct: 421 IGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVS 480

Query: 481 TEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTF 540
           T+PGHY IFWE+SGE  N+D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF
Sbjct: 481 TDPGHYAIFWEISGET-NEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 540

Query: 541 QKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           +KI +HFL LG++  QFK PRC+ P+N KVLQILC NV   YF
Sbjct: 541 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYF 571

BLAST of Lag0001800 vs. ExPASy Swiss-Prot
Match: Q53P49 (Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.12 PE=2 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 3.9e-173
Identity = 309/606 (50.99%), Postives = 418/606 (68.98%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEE----FEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDP 60
           MLEK  +  M +  EE    FE  T+DA  VQRETL+ IL EN   EYL+ LGL G TD 
Sbjct: 1   MLEKEGKLIMSREDEEIMAWFERTTRDAADVQRETLRRILAENAGVEYLRELGLAGLTDA 60

Query: 61  VSFKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELI 120
            SF+A VP+VTH DL+PYIQR+ADG  +P+LT KP+T ISLSSGTTQGK K + FND+L+
Sbjct: 61  GSFRARVPVVTHADLDPYIQRVADGDASPVLTAKPVTAISLSSGTTQGKRKRLLFNDDLL 120

Query: 121 ETTIQIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTM 180
            ++I+ F  S+AF NR FP+E GR L F+YGS+   T G L A T  +NL R   F ++M
Sbjct: 121 RSSIRFFHASYAFTNRAFPVEDGRVLQFMYGSRHETTKGGLTATTVMTNLLRSEEFTASM 180

Query: 181 KAIMS---TTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFES 240
            A       +CSP EV+F  DF +SLYCHLLCGL+   E++ + ++FAHSIV A +  E 
Sbjct: 181 AARSRPRLPSCSPSEVVFSPDFDESLYCHLLCGLLLAGEVRAVSASFAHSIVVALQALER 240

Query: 241 IWEELCCDIQSGVLS-SRITSPSIRAGMLKLL-KPNPELANLVYEKCVGLSNWYGLIPQL 300
           +W ELC DI+ G  S +R+T+P++R  +  +L  PNP LA+ +  +C  L +W G+IP L
Sbjct: 241 VWRELCADIRRGAASPARVTTPAVRRAVAPILAAPNPALADALERRCAALGDWSGVIPAL 300

Query: 301 FPNAKYIYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTV 360
           +PNA+Y+   MTGSME YVKKLRHYAG +PL+S +Y SSEG IG N     PPE   FTV
Sbjct: 301 WPNARYVQATMTGSMEHYVKKLRHYAGGVPLVSGNYASSEGVIGINAEQHAPPESVVFTV 360

Query: 361 LPNIGYFEFIPL----GDDIFEHDK-TQSSSIC----NELKPVDLTRVQVGKEYEILITS 420
           LP+  YFEFIPL     D   + D    + S C    ++  PV LT V VG+ YE+++T+
Sbjct: 361 LPDAAYFEFIPLKPPCTDAAADDDNPAAAGSSCYVDADDANPVGLTDVVVGEHYEVVMTT 420

Query: 421 VAGLYRYRLGDVVKVTGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLA- 480
             GLYRYRLGDVVKV GFH++TP L+FVCRR+L+L+IN+DKN+E DLQ+AV+ A+K+LA 
Sbjct: 421 FTGLYRYRLGDVVKVAGFHHATPKLRFVCRRSLILSINVDKNSEHDLQLAVDSAAKILAG 480

Query: 481 ----EEKLEVVDFTSYVDRSTEPGHYVIFWEMSGEAYND--DILKQCCNCLDSSF-VDAG 540
                ++LE+ D+TS+ D S++PGHYV+FWE++G    D   +L++CC+ +D +F  DAG
Sbjct: 481 DGENHKQLEIADYTSHADTSSDPGHYVVFWELNGGGEEDGGGVLQRCCDEMDRAFGADAG 540

Query: 541 YVSSRRVKAIGALELRVVQRGTFQKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSN 581
           Y  SR+  AIGALELRV++RG FQ++L H+++ G++  QFK PRC+ P+N  VL++L  N
Sbjct: 541 YAQSRKTCAIGALELRVLRRGAFQEVLRHYVAGGSSAGQFKMPRCVAPSNAGVLRVLKDN 600

BLAST of Lag0001800 vs. ExPASy TrEMBL
Match: A0A6J1DWL3 (jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025068 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 2.7e-302
Identity = 515/580 (88.79%), Postives = 549/580 (94.66%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEE E ++K+A RVQRETLK ILEENGS+EYLQNLGLNG TDP SFK
Sbjct: 1   MLEKMEEFDTEKVIEEVEAMSKEAGRVQRETLKRILEENGSSEYLQNLGLNGNTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
           ACVPIVTHEDLEPYIQRIAD   A ILT KPITTISLSSGTT+GKPKFVPFNDEL+++TI
Sbjct: 61  ACVPIVTHEDLEPYIQRIADSHHASILTAKPITTISLSSGTTEGKPKFVPFNDELVDSTI 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
           QIF+ SFAFRNRDFPI KGRSLMFIYGSKQFKTNG L AATATSNLYRDP+FKSTMK+IM
Sbjct: 121 QIFQASFAFRNRDFPIGKGRSLMFIYGSKQFKTNGGLTAATATSNLYRDPKFKSTMKSIM 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +CSPDEV++GSDFHQSLYCHLLCGLIFRDEIQ+IFSTFAHSIVHAFRTFE IWEELCC
Sbjct: 181 SMSCSPDEVIYGSDFHQSLYCHLLCGLIFRDEIQLIFSTFAHSIVHAFRTFEHIWEELCC 240

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI++G+L+SRITSPSIRAGM  LLKPNPELA+LVYEKCVGLS WYGLIPQLFPNAKYIYG
Sbjct: 241 DIRNGILTSRITSPSIRAGMSNLLKPNPELADLVYEKCVGLSTWYGLIPQLFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPYVKKLRHYAG+LPLMSADYGSSEGWIGAN NPK+ PEFATFTVLPNIGYFEF
Sbjct: 301 IMTGSMEPYVKKLRHYAGDLPLMSADYGSSEGWIGANINPKLSPEFATFTVLPNIGYFEF 360

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPLG +I E+DK+QSS IC+ELKPVDLT+VQVGKEYEILITSVAG+YRYRLGDVVKVTGF
Sbjct: 361 IPLG-EISENDKSQSSLICSELKPVDLTQVQVGKEYEILITSVAGMYRYRLGDVVKVTGF 420

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           +NSTP LKFVCRRNLLLTINIDKNTEKDLQIAV EASKLLAEEKLEVVDFTSYVDRST+P
Sbjct: 421 YNSTPELKFVCRRNLLLTINIDKNTEKDLQIAVGEASKLLAEEKLEVVDFTSYVDRSTDP 480

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWEMSGEAYNDD+L +CCN LD+SFVDAGYVSSRRVK+IGALELRVVQRGTFQKI
Sbjct: 481 GHYVIFWEMSGEAYNDDLLNRCCNRLDTSFVDAGYVSSRRVKSIGALELRVVQRGTFQKI 540

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNV KVYF
Sbjct: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVIKVYF 579

BLAST of Lag0001800 vs. ExPASy TrEMBL
Match: A0A6J1DWB6 (jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025068 PE=3 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 7.2e-279
Identity = 487/580 (83.97%), Postives = 516/580 (88.97%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEE E ++K+A RVQRETLK ILEENGS+EYLQNLGLNG TDP SFK
Sbjct: 1   MLEKMEEFDTEKVIEEVEAMSKEAGRVQRETLKRILEENGSSEYLQNLGLNGNTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
           ACVPIVTHEDLEPYIQRIAD   A ILT KPITTISL                       
Sbjct: 61  ACVPIVTHEDLEPYIQRIADSHHASILTAKPITTISL----------------------- 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
                      RDFPI KGRSLMFIYGSKQFKTNG L AATATSNLYRDP+FKSTMK+IM
Sbjct: 121 -----------RDFPIGKGRSLMFIYGSKQFKTNGGLTAATATSNLYRDPKFKSTMKSIM 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +CSPDEV++GSDFHQSLYCHLLCGLIFRDEIQ+IFSTFAHSIVHAFRTFE IWEELCC
Sbjct: 181 SMSCSPDEVIYGSDFHQSLYCHLLCGLIFRDEIQLIFSTFAHSIVHAFRTFEHIWEELCC 240

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI++G+L+SRITSPSIRAGM  LLKPNPELA+LVYEKCVGLS WYGLIPQLFPNAKYIYG
Sbjct: 241 DIRNGILTSRITSPSIRAGMSNLLKPNPELADLVYEKCVGLSTWYGLIPQLFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPYVKKLRHYAG+LPLMSADYGSSEGWIGAN NPK+ PEFATFTVLPNIGYFEF
Sbjct: 301 IMTGSMEPYVKKLRHYAGDLPLMSADYGSSEGWIGANINPKLSPEFATFTVLPNIGYFEF 360

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPLG +I E+DK+QSS IC+ELKPVDLT+VQVGKEYEILITSVAG+YRYRLGDVVKVTGF
Sbjct: 361 IPLG-EISENDKSQSSLICSELKPVDLTQVQVGKEYEILITSVAGMYRYRLGDVVKVTGF 420

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           +NSTP LKFVCRRNLLLTINIDKNTEKDLQIAV EASKLLAEEKLEVVDFTSYVDRST+P
Sbjct: 421 YNSTPELKFVCRRNLLLTINIDKNTEKDLQIAVGEASKLLAEEKLEVVDFTSYVDRSTDP 480

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWEMSGEAYNDD+L +CCN LD+SFVDAGYVSSRRVK+IGALELRVVQRGTFQKI
Sbjct: 481 GHYVIFWEMSGEAYNDDLLNRCCNRLDTSFVDAGYVSSRRVKSIGALELRVVQRGTFQKI 540

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNV KVYF
Sbjct: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVIKVYF 545

BLAST of Lag0001800 vs. ExPASy TrEMBL
Match: A0A2I4E0S1 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 OS=Juglans regia OX=51240 GN=LOC108985239 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 2.0e-268
Identity = 448/582 (76.98%), Postives = 517/582 (88.83%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 11  MLEKMEEFDPEKVIEEFEAITRDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 70

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTH++LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 71  TCVPLVTHKELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 130

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G+ L FIY SKQF+T G L A TAT+N+YR+ +FK TMKAI 
Sbjct: 131 QIYRTSFAFRNREFPIGNGKGLQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 190

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 191 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSILHAFRTFEQVWEELCT 250

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLS+R+++PSIRA M KLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 251 DIREGVLSNRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 310

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+PPE ATF VLPNIG+FEF
Sbjct: 311 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPESATFAVLPNIGFFEF 370

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT V++G++YEI++T+ AGLYRY+LGDVVKV 
Sbjct: 371 IPLKHNIEDQDQNQMDPSFFQIEHEPVDLTEVKIGEDYEIIVTNFAGLYRYKLGDVVKVM 430

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 431 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 490

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SF+DAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 491 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFIDAGYVSSRKVNAIGALELRVVRRGTFQ 550

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           KILDH+L LG+A+SQFKTPRC+GP NN VLQILC NV + YF
Sbjct: 551 KILDHYLGLGSALSQFKTPRCVGPTNNLVLQILCGNVVESYF 591

BLAST of Lag0001800 vs. ExPASy TrEMBL
Match: A0A6P9E9C2 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X1 OS=Juglans regia OX=51240 GN=LOC108985239 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 2.0e-268
Identity = 448/582 (76.98%), Postives = 517/582 (88.83%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 46  MLEKMEEFDPEKVIEEFEAITRDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 105

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTH++LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 106 TCVPLVTHKELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 165

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G+ L FIY SKQF+T G L A TAT+N+YR+ +FK TMKAI 
Sbjct: 166 QIYRTSFAFRNREFPIGNGKGLQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 225

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 226 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSILHAFRTFEQVWEELCT 285

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLS+R+++PSIRA M KLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 286 DIREGVLSNRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 345

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+PPE ATF VLPNIG+FEF
Sbjct: 346 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPESATFAVLPNIGFFEF 405

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT V++G++YEI++T+ AGLYRY+LGDVVKV 
Sbjct: 406 IPLKHNIEDQDQNQMDPSFFQIEHEPVDLTEVKIGEDYEIIVTNFAGLYRYKLGDVVKVM 465

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 466 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 525

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SF+DAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 526 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFIDAGYVSSRKVNAIGALELRVVRRGTFQ 585

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           KILDH+L LG+A+SQFKTPRC+GP NN VLQILC NV + YF
Sbjct: 586 KILDHYLGLGSALSQFKTPRCVGPTNNLVLQILCGNVVESYF 626

BLAST of Lag0001800 vs. ExPASy TrEMBL
Match: A0A2I4E0R7 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 OS=Juglans regia OX=51240 GN=LOC108985239 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 2.0e-268
Identity = 448/582 (76.98%), Postives = 517/582 (88.83%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 1   MLEKMEEFDPEKVIEEFEAITRDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTH++LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 61  TCVPLVTHKELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G+ L FIY SKQF+T G L A TAT+N+YR+ +FK TMKAI 
Sbjct: 121 QIYRTSFAFRNREFPIGNGKGLQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 181 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSILHAFRTFEQVWEELCT 240

Query: 241 DIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLS+R+++PSIRA M KLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 241 DIREGVLSNRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+PPE ATF VLPNIG+FEF
Sbjct: 301 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPESATFAVLPNIGFFEF 360

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT V++G++YEI++T+ AGLYRY+LGDVVKV 
Sbjct: 361 IPLKHNIEDQDQNQMDPSFFQIEHEPVDLTEVKIGEDYEIIVTNFAGLYRYKLGDVVKVM 420

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 421 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 480

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SF+DAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 481 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFIDAGYVSSRKVNAIGALELRVVRRGTFQ 540

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           KILDH+L LG+A+SQFKTPRC+GP NN VLQILC NV + YF
Sbjct: 541 KILDHYLGLGSALSQFKTPRCVGPTNNLVLQILCGNVVESYF 581

BLAST of Lag0001800 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 789.6 bits (2038), Expect = 2.0e-228
Identity = 373/583 (63.98%), Postives = 474/583 (81.30%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGK-TDP-VS 60
           MLEK+E FDM +VI+EF+E+T++A +VQ++TLK IL +N SA YLQN GLNG  TDP  +
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIET 120
           FK+ VP+VT  +LEPYI+R+ DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TIQIFRTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMK 180
           T+Q+FRT+FAFRNRDFPI + G++L FI+ SKQ+ + G +   TAT+N+YR+P FK+ MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEE 240
           +I S +CSPDEV+F  D HQ+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCCDIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKY 300
           +  DI+ GVLS+RIT PS+R  M KLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGY 360
           +YGIMTGSMEPYV KLRHYAG+LPL+S DYGSSEGWI AN  P++ PE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKV 420
           FEF+P+           S +   E KPV LT+V++G+EYE++IT+ AGLYRYRLGDVVKV
Sbjct: 361 FEFLPV-----------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKV 420

Query: 421 TGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRS 480
            GF+N+TP LKF+CRRNL+L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D S
Sbjct: 421 IGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVS 480

Query: 481 TEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTF 540
           T+PGHY IFWE+SGE  N+D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF
Sbjct: 481 TDPGHYAIFWEISGET-NEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 540

Query: 541 QKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           +KI +HFL LG++  QFK PRC+ P+N KVLQILC NV   YF
Sbjct: 541 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYF 571

BLAST of Lag0001800 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 789.6 bits (2038), Expect = 2.0e-228
Identity = 373/583 (63.98%), Postives = 474/583 (81.30%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGK-TDP-VS 60
           MLEK+E FDM +VI+EF+E+T++A +VQ++TLK IL +N SA YLQN GLNG  TDP  +
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIET 120
           FK+ VP+VT  +LEPYI+R+ DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TIQIFRTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMK 180
           T+Q+FRT+FAFRNRDFPI + G++L FI+ SKQ+ + G +   TAT+N+YR+P FK+ MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEE 240
           +I S +CSPDEV+F  D HQ+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCCDIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKY 300
           +  DI+ GVLS+RIT PS+R  M KLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGY 360
           +YGIMTGSMEPYV KLRHYAG+LPL+S DYGSSEGWI AN  P++ PE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKV 420
           FEF+P+           S +   E KPV LT+V++G+EYE++IT+ AGLYRYRLGDVVKV
Sbjct: 361 FEFLPV-----------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKV 420

Query: 421 TGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRS 480
            GF+N+TP LKF+CRRNL+L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D S
Sbjct: 421 IGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVS 480

Query: 481 TEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTF 540
           T+PGHY IFWE+SGE  N+D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF
Sbjct: 481 TDPGHYAIFWEISGET-NEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 540

Query: 541 QKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           +KI +HFL LG++  QFK PRC+ P+N KVLQILC NV   YF
Sbjct: 541 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYF 571

BLAST of Lag0001800 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 789.6 bits (2038), Expect = 2.0e-228
Identity = 373/583 (63.98%), Postives = 474/583 (81.30%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGK-TDP-VS 60
           MLEK+E FDM +VI+EF+E+T++A +VQ++TLK IL +N SA YLQN GLNG  TDP  +
Sbjct: 12  MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 71

Query: 61  FKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIET 120
           FK+ VP+VT  +LEPYI+R+ DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E 
Sbjct: 72  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 131

Query: 121 TIQIFRTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMK 180
           T+Q+FRT+FAFRNRDFPI + G++L FI+ SKQ+ + G +   TAT+N+YR+P FK+ MK
Sbjct: 132 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 191

Query: 181 AIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEE 240
           +I S +CSPDEV+F  D HQ+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE
Sbjct: 192 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 251

Query: 241 LCCDIQSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKY 300
           +  DI+ GVLS+RIT PS+R  M KLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY
Sbjct: 252 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 311

Query: 301 IYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGY 360
           +YGIMTGSMEPYV KLRHYAG+LPL+S DYGSSEGWI AN  P++ PE ATF V+PN+GY
Sbjct: 312 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 371

Query: 361 FEFIPLGDDIFEHDKTQSSSICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKV 420
           FEF+P+           S +   E KPV LT+V++G+EYE++IT+ AGLYRYRLGDVVKV
Sbjct: 372 FEFLPV-----------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKV 431

Query: 421 TGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRS 480
            GF+N+TP LKF+CRRNL+L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D S
Sbjct: 432 IGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVS 491

Query: 481 TEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTF 540
           T+PGHY IFWE+SGE  N+D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF
Sbjct: 492 TDPGHYAIFWEISGET-NEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 551

Query: 541 QKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYF 581
           +KI +HFL LG++  QFK PRC+ P+N KVLQILC NV   YF
Sbjct: 552 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYF 582

BLAST of Lag0001800 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 706.4 bits (1822), Expect = 2.2e-203
Identity = 325/504 (64.48%), Postives = 410/504 (81.35%), Query Frame = 0

Query: 78  IADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTIQIFRTSFAFRNRDFPI- 137
           + DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E T+Q+FRT+FAFRNRDFPI 
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 138 EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIMSTTCSPDEVMFGSDFH 197
           + G++L FI+ SKQ+ + G +   TAT+N+YR+P FK+ MK+I S +CSPDEV+F  D H
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 198 QSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCCDIQSGVLSSRITSPSI 257
           Q+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE+  DI+ GVLS+RIT PS+
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 258 RAGMLKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYGIMTGSMEPYVKKLRHY 317
           R  M KLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY+YGIMTGSMEPYV KLRHY
Sbjct: 181 RTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHY 240

Query: 318 AGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFEFIPLGDDIFEHDKTQSS 377
           AG+LPL+S DYGSSEGWI AN  P++ PE ATF V+PN+GYFEF+P+           S 
Sbjct: 241 AGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPV-----------SE 300

Query: 378 SICNELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDVVKVTGFHNSTPVLKFVCRRNLL 437
           +   E KPV LT+V++G+EYE++IT+ AGLYRYRLGDVVKV GF+N+TP LKF+CRRNL+
Sbjct: 301 TGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLI 360

Query: 438 LTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEPGHYVIFWEMSGEAYND 497
           L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D ST+PGHY IFWE+SGE  N+
Sbjct: 361 LSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYAIFWEISGET-NE 420

Query: 498 DILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKILDHFLSLGAAVSQFKT 557
           D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF+KI +HFL LG++  QFK 
Sbjct: 421 DVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKM 480

Query: 558 PRCIGPANNKVLQILCSNVNKVYF 581
           PRC+ P+N KVLQILC NV   YF
Sbjct: 481 PRCVKPSNAKVLQILCENVVSSYF 492

BLAST of Lag0001800 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 581.3 bits (1497), Expect = 1.1e-165
Identity = 302/578 (52.25%), Postives = 399/578 (69.03%), Query Frame = 0

Query: 11  EKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQN-LG------LNGKTDPVSFKACV 70
           + VI  FE ++++A +VQ ETL+ ILE N   EYL+  LG      ++  T    F + V
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKMDDYTLETLFTSLV 68

Query: 71  PIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTIQIF 130
           PIV+H DL+PYIQRIADG  +P+LT +PIT +SLSSGTT+G+ K+VPF     +TT+QIF
Sbjct: 69  PIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQIF 128

Query: 131 RTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRDPRFKSTMKAIMST 190
           R S A+R+R +PI E GR L FIY  K+FKT G L   TAT++ Y    FK+  +   S 
Sbjct: 129 RLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKSF 188

Query: 191 TCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFESIWEELCCDI 250
           TCSP EV+ G DF Q  YCHLL GL +  +++ + S F+++IV AF  FE IW E+C DI
Sbjct: 189 TCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICADI 248

Query: 251 QSGVLSSRITSPSIRAGMLKLLKPNPELANLVYEKCVGLS---NWYGLIPQLFPNAKYIY 310
           + G LSSRIT P +R  +L L++PNP LA+ + E C+ L     W+GLI +L+PNAK+I 
Sbjct: 249 KEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFIS 308

Query: 311 GIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMPPEFATFTVLPNIGYFE 370
            IMTGSM PY+ KLRHYAG LPL+SADYGS+E WIG N +P +PPE  +F V+P   YFE
Sbjct: 309 SIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYFE 368

Query: 371 FIPLGDDIFEHDKTQSSSIC-----NELKPVDLTRVQVGKEYEILITSVAGLYRYRLGDV 430
           FIPL      + +   S IC      E KPV L++V++G+EYE+++T+  GLYRYRLGDV
Sbjct: 369 FIPL------YRRQNQSDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDV 428

Query: 431 VKVTGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEE-KLEVVDFTSY 490
           V+VT FH  TP L F+ RR L+LTINIDKNTEKDLQ  V++AS+LL+   + EVVDFTS+
Sbjct: 429 VEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSH 488

Query: 491 VDRSTEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQ 550
            D    PGHYVI+WE+ GEA +D  L++CC  +D++FVD GYV SRR+ +IG LELRVV+
Sbjct: 489 ADVIARPGHYVIYWEIRGEA-DDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVE 548

Query: 551 RGTFQKILDHFLSLGAAVSQFKTPRCIGPANNKVLQIL 572
           RGTF K+ +  +     ++QFKTPRC    N+ +L IL
Sbjct: 549 RGTFGKVAERCVGKCGGLNQFKTPRC--TTNSVMLDIL 577

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158613.15.6e-30288.79jasmonic acid-amido synthetase JAR1-like isoform X1 [Momordica charantia][more]
XP_022158615.11.5e-27883.97jasmonic acid-amido synthetase JAR1-like isoform X2 [Momordica charantia][more]
XP_041001957.13.3e-27077.84jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 [Juglans microcarpa x Ju... [more]
XP_041001955.13.3e-27077.84jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 [Juglans microcarpa x Ju... [more]
XP_041001954.13.3e-27077.84jasmonoyl--L-amino acid synthetase JAR4-like isoform X1 [Juglans microcarpa x Ju... [more]
Match NameE-valueIdentityDescription
A0A314KSQ41.2e-25172.07Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ... [more]
A0A1J6KGJ91.8e-25071.38Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ... [more]
Q6I5814.3e-23665.96Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q9SKE22.8e-22763.98Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ... [more]
Q53P493.9e-17350.99Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. jap... [more]
Match NameE-valueIdentityDescription
A0A6J1DWL32.7e-30288.79jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Momordica charantia OX=36... [more]
A0A6J1DWB67.2e-27983.97jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Momordica charantia OX=36... [more]
A0A2I4E0S12.0e-26876.98jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 OS=Juglans regia OX=5124... [more]
A0A6P9E9C22.0e-26876.98jasmonoyl--L-amino acid synthetase JAR4-like isoform X1 OS=Juglans regia OX=5124... [more]
A0A2I4E0R72.0e-26876.98jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 OS=Juglans regia OX=5124... [more]
Match NameE-valueIdentityDescription
AT2G46370.12.0e-22863.98Auxin-responsive GH3 family protein [more]
AT2G46370.22.0e-22863.98Auxin-responsive GH3 family protein [more]
AT2G46370.42.0e-22863.98Auxin-responsive GH3 family protein [more]
AT2G46370.32.2e-20364.48Auxin-responsive GH3 family protein [more]
AT4G03400.11.1e-16552.25Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 10..30
NoneNo IPR availableCOILSCoilCoilcoord: 446..466
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 609..624
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 640..671
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 625..639
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 592..694
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 672..694
NoneNo IPR availablePANTHERPTHR31901:SF40JASMONIC ACID-AMIDO SYNTHETASE JAR1-LIKE ISOFORM X1coord: 6..591
IPR004993GH3 familyPFAMPF03321GH3coord: 16..560
e-value: 1.8E-183
score: 611.2
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 6..591

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0001800.1Lag0001800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016881 acid-amino acid ligase activity
molecular_function GO:0102053 (-)-jasmonoyl-isoleucine synthetase activity
molecular_function GO:0102058 jasmonoyl-leucine synthetase activity
molecular_function GO:0102057 jasmonoyl-valine synthetase activity