Lag0001710 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0001710 vs. NCBI nr
Match: XP_038874970.1 (uncharacterized protein LOC120067490 [Benincasa hispida])

HSP 1 Score: 422.5 bits (1085), Expect = 3.3e-114
Identity = 213/321 (66.36%), Postives = 260/321 (81.00%), Query Frame = 0

Query: 21  DSASTTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKWKLAL 80
           ++ +++  +R+SRD+KPLHYTL+IQSFSLLKAA+A+S+  R+RYESQ F +GGYKWKLAL
Sbjct: 19  NNENSSLVIRTSRDEKPLHYTLKIQSFSLLKAAVAASS-IRNRYESQIFKAGGYKWKLAL 78

Query: 81  YPNGDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGKVRRF 140
           YPNGD KRN ++HISLYLV AED++  ++  E+ VM  FLVYD+LKD+Y T+ DGKVR F
Sbjct: 79  YPNGDFKRNVANHISLYLVSAEDEIS-SSGLEIKVMIKFLVYDNLKDRYWTIQDGKVRCF 138

Query: 141 HAIKKEWGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMK-FEGNGEVLSFIKQPKDYK 200
           HAIKKEWGFEKLVSLDTFND S+GLLVDDCCAFGVDVIVMK ++GN E+LS IKQ K+YK
Sbjct: 139 HAIKKEWGFEKLVSLDTFNDTSNGLLVDDCCAFGVDVIVMKSYDGNNEILSLIKQLKNYK 198

Query: 201 FTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQG 260
           +TWK  NFS    N YESDPFTV+NY WKI LYP GDS A TG++S++LR DS +++PQG
Sbjct: 199 YTWKINNFSQLSKNVYESDPFTVDNYTWKIELYPRGDSLAMTGYMSMFLRFDSFEKLPQG 258

Query: 261 FKVYVESEMAVLSQLGVGHRRYLTQKHWFEGE-GGRGWSEFMPLSNLKKPMKGFLHDDTL 320
            +VYVE ++AVLSQLG  +  Y   KHWF+GE    G+  FM LSN+K+PMKGFL DDTL
Sbjct: 259 SRVYVEYDIAVLSQLGGAYTNY-PVKHWFDGETKSWGFGNFMSLSNVKEPMKGFLCDDTL 318

Query: 321 MVEVKINVISTSKKIHRKENG 340
           MVEVKINVIS  K  +RK NG
Sbjct: 319 MVEVKINVISILKSFYRKGNG 336

BLAST of Lag0001710 vs. NCBI nr
Match: XP_038875772.1 (uncharacterized protein LOC120068142 [Benincasa hispida])

HSP 1 Score: 381.7 bits (979), Expect = 6.5e-102
Identity = 204/339 (60.18%), Postives = 256/339 (75.52%), Query Frame = 0

Query: 1   MEEKKRKTNDNFSPYPTPNPDSASTTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQ 60
           M + +RK N+N     +PNP   +++S V S+R +KP+HYTL+IQSFSLLK  LASS  Q
Sbjct: 1   MGKGRRKNNNN---TVSPNPHGDNSSSVVMSTRHEKPVHYTLKIQSFSLLKNGLASS--Q 60

Query: 61  RDRYESQTFNSGGYKWKLALYPNGDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFL 120
           R R+ESQ FN+G Y+WKLALYPNGD++RN S+HISLYLV+  +++L +TTSEVNV+FTFL
Sbjct: 61  RQRFESQKFNAGCYEWKLALYPNGDERRNVSNHISLYLVIVGNNIL-STTSEVNVVFTFL 120

Query: 121 VYDHLKDKYLTVHDGKVRRFHAIKKEWGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVM 180
           VYD L+ KYLTV DGK+RRF A+K EWG EKL+ LDTFNDAS+G LVDDCC FGVD+ VM
Sbjct: 121 VYDTLRGKYLTVQDGKMRRFSAMKTEWGIEKLLPLDTFNDASNGFLVDDCCVFGVDIFVM 180

Query: 181 KFEGN-GEVLSFIKQPKDYKFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRA 240
             +G  GEV S IKQPK+Y++TWK  NFS       ES+PFTVE+YRWKI LYP+G S+A
Sbjct: 181 SCDGGMGEVFSLIKQPKNYEYTWKLNNFSQLGSTLKESNPFTVESYRWKILLYPSGCSKA 240

Query: 241 RTGFLSLYLRLDSLKEVPQGFKVYVESEMAVLSQLGVGHRRYLTQKHWFEGEG---GRGW 300
           ++GFLS+YL  DS  ++PQG +VYVE EMA+LSQL     +  T   WF  +G   GRGW
Sbjct: 241 KSGFLSMYLLFDSSNQLPQGSQVYVEYEMAILSQLEAVPVKE-TYNCWFGFDGVEAGRGW 300

Query: 301 S--EFMPLSNLKKPMKGFLHDDTLMVEVKINVISTSKKI 334
               F  LS+L++P KG+L +DTL+ EVKINVIST KKI
Sbjct: 301 GAPNFSSLSDLEEPTKGYLLNDTLVTEVKINVISTLKKI 332

BLAST of Lag0001710 vs. NCBI nr
Match: KAE8648447.1 (hypothetical protein Csa_008888 [Cucumis sativus])

HSP 1 Score: 366.3 bits (939), Expect = 2.8e-97
Identity = 197/337 (58.46%), Postives = 244/337 (72.40%), Query Frame = 0

Query: 4   KKRKTNDNFSPYP-TPNPDSASTTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRD 63
           K R+ N N + +P +   D +++ S V SSR +KP+HY L+IQSFSLLK  LA S   R 
Sbjct: 3   KGRRKNKNINVFPKSHGHDDSASASVVTSSRYEKPVHYILKIQSFSLLKEGLACS--PRQ 62

Query: 64  RYESQTFNSGGYKWKLALYPNGDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVY 123
           R+ESQ FN+GGY+WKLALYPNGD +R+ SDHISLYLVM  D++L +TTSEVN +FTFLVY
Sbjct: 63  RFESQKFNAGGYEWKLALYPNGDQRRDVSDHISLYLVMVGDNIL-STTSEVNAVFTFLVY 122

Query: 124 DHLKDKYLTVHDGKVRRFHAIKKEWGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMKF 183
           D L+ KYLTV DGK+RRF A K EWG EKL+ L+TF DAS+G LVDDCC FGVD+ VM  
Sbjct: 123 DTLRGKYLTVQDGKMRRFSATKTEWGIEKLLPLNTFKDASNGFLVDDCCVFGVDIFVMNS 182

Query: 184 E-GNGEVLSFIKQPKDYKFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRART 243
           + G GEV S I+QP +YK+TWK  NFS    +  E +PFTVEN  WKI L+P+GD +A+ 
Sbjct: 183 DVGKGEVFSLIEQPNNYKYTWKLNNFSKLDSSLRECNPFTVENCCWKIRLFPSGDLQAKP 242

Query: 244 GFLSLYLRLDSLKEVPQGFKVYVESEMAVLSQLGVGHRRYLTQKHWF-----EGEGGRGW 303
           GF S+YL L +LKE PQG +VYVE EMAVLSQL        T   WF     E + G G 
Sbjct: 243 GFFSMYLMLTNLKEFPQGAQVYVEYEMAVLSQLEDVPPIKETYICWFGFGGIEADKGCGA 302

Query: 304 SEFMPLSNLKKPMKGFLHDDTLMVEVKINVISTSKKI 334
           S F+ LS+LK+P +G+L +DTL++EVKINVIST KKI
Sbjct: 303 SNFLSLSDLKEPARGYLLNDTLVIEVKINVISTLKKI 336

BLAST of Lag0001710 vs. NCBI nr
Match: XP_022146380.1 (probable inactive serine/threonine-protein kinase fnkC [Momordica charantia])

HSP 1 Score: 357.1 bits (915), Expect = 1.7e-94
Identity = 183/307 (59.61%), Postives = 219/307 (71.34%), Query Frame = 0

Query: 29  VRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKWKLALYPNGDDKR 88
           +RS+RD+KPLHYTLQIQSFSLLKAALASSN  RDRYESQ FN+GGYKWKLA+YPNGD KR
Sbjct: 9   IRSTRDEKPLHYTLQIQSFSLLKAALASSN--RDRYESQIFNAGGYKWKLAVYPNGDVKR 68

Query: 89  NGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGKVRRFHAIKKEWG 148
           NG+DHISLYLV A+DD+                             GKVRRFHA+K EWG
Sbjct: 69  NGADHISLYLVKADDDI------------------------NPCSHGKVRRFHAMKAEWG 128

Query: 149 FEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMKFEGNGEVLSFIKQPKDYKFTWKFTNFS 208
           FEKL+SLDTFND+S+G +V DCC FGVDV+VMK +G GEV+SFIK+PK +K+TWK  NFS
Sbjct: 129 FEKLISLDTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKTKNFS 188

Query: 209 LNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQGFKVYVESEM 268
                +YESDPFTVE YRWK+ LYP G S+A+TGFLSLYL  +S +E+PQG +VYVE E 
Sbjct: 189 QLDKKFYESDPFTVEGYRWKLWLYPKGSSKAKTGFLSLYLSYNSYEEIPQGSQVYVEYED 248

Query: 269 AVLSQLGVGHRRYLTQKHWFEGEGGRGWS--EFMPLSNLKKPMKGFLHDDTLMVEVKINV 328
           +     G G             E GRGW    F+ L +L +P +GF+ DDT++VEVKINV
Sbjct: 249 SHNCWFGFG-----------GVESGRGWGHPNFLSLRDLNEPTEGFIVDDTIIVEVKINV 278

Query: 329 ISTSKKI 334
           +ST KKI
Sbjct: 309 VSTLKKI 278

BLAST of Lag0001710 vs. NCBI nr
Match: XP_022146382.1 (uncharacterized protein LOC111015610 [Momordica charantia])

HSP 1 Score: 350.1 bits (897), Expect = 2.1e-92
Identity = 189/335 (56.42%), Postives = 236/335 (70.45%), Query Frame = 0

Query: 4   KKRKTNDNFSPYPTPNPDSASTTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDR 63
           K+ + N   SP        A + S +RS RD+KPLHYTLQIQ FSLLK ALA+S+  RDR
Sbjct: 34  KRMQRNHIISP-----SSQADSGSVLRSIRDEKPLHYTLQIQPFSLLKTALATSS--RDR 93

Query: 64  YESQTFNSGGYKWKLALYPNGDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYD 123
           YES  F+  G+KWKLA+YP+GD KRNGS+HISLYLV AEDD+    T ++ V+FT LVYD
Sbjct: 94  YESPIFSVAGFKWKLAVYPSGDVKRNGSNHISLYLVTAEDDI---PTLDLTVVFTILVYD 153

Query: 124 HLKDKYLTVHDGKVRRFHAIKKEWGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMKFE 183
           +L+D YL V DGKVRRFHA+KKEWGFEKLVSL+TFND+S+G LVDDCCAFGVD+ VMK +
Sbjct: 154 NLRDNYLAVLDGKVRRFHAMKKEWGFEKLVSLETFNDSSNGFLVDDCCAFGVDIFVMKCD 213

Query: 184 GNGEVLSFIKQPKDYKFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGF 243
           G GE LS IKQPK+ K+TWK  NFS    N              K+ LYP G  +A   +
Sbjct: 214 GKGEALSLIKQPKNNKYTWKTNNFSQLDEN-------------MKMSLYPKGSPKASPDY 273

Query: 244 LSLYLRLDSLKEVPQGFKVYVESEMAVLSQLGVGHRRYLTQKHWFE-GEGGRGWSEFMPL 303
           LSL+LR +SLKE+PQG +VY E E+AVLS            K+WFE G+ G G+ +FM L
Sbjct: 274 LSLFLRCESLKELPQGCRVYAEFEIAVLS------------KYWFEFGDLGLGFPDFMSL 333

Query: 304 SNLKKPMKGFLHDDTLMVEVKINVISTSKKIHRKE 338
            +L +  KG++ DDTL+VEVKINV+ST K++ +KE
Sbjct: 334 RDLNEVTKGYICDDTLIVEVKINVVSTLKELFKKE 333

BLAST of Lag0001710 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 8.8e-17
Identity = 49/138 (35.51%), Postives = 76/138 (55.07%), Query Frame = 0

Query: 193 KQPKDYKFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDS 252
           + P   KFTW   NFS      + SD F V  Y+W+I ++P G++      LS+YL +  
Sbjct: 50  EDPPTLKFTWTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKGNN---VDHLSMYLDVSD 109

Query: 253 LKEVPQGFKVYVESEMAVLSQLGVGHRRYLTQK---HWFEG-EGGRGWSEFMPLSNLKKP 312
              +P G+  Y +  +AV++Q+   H RY  +K   H F   E   G++ FMPLS L  P
Sbjct: 110 AASLPYGWSRYAQFSLAVVNQI---HTRYTVRKETQHQFNARESDWGFTSFMPLSELYDP 169

Query: 313 MKGFLHDDTLMVEVKINV 327
            +G+L +DT++VE ++ V
Sbjct: 170 SRGYLVNDTVLVEAEVAV 181

BLAST of Lag0001710 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 7.0e-14
Identity = 43/135 (31.85%), Postives = 74/135 (54.81%), Query Frame = 0

Query: 193 KQPKDYKFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDS 252
           + P   KFTW    F+      + SD F V  Y+W+I ++P G++      LS+YL +  
Sbjct: 49  EDPPSLKFTWTIPMFTRLNTRKHYSDVFVVGGYKWRILIFPKGNN---VDHLSMYLDVAD 108

Query: 253 LKEVPQGFKVYVESEMAVLSQLGVGHR-RYLTQKHWFEGEGGRGWSEFMPLSNLKKPMKG 312
              +P G+  Y +  +AV++Q+   +  R  TQ  +   E   G++ FMPLS L +P +G
Sbjct: 109 AANLPYGWSRYSQFSLAVVNQVNNRYSIRKETQHQFNARESDWGFTSFMPLSELYEPTRG 168

Query: 313 FLHDDTLMVEVKINV 327
           +L +DT+++E ++ V
Sbjct: 169 YLVNDTVLIEAEVAV 180

BLAST of Lag0001710 vs. ExPASy Swiss-Prot
Match: Q9M2H9 (MATH domain and coiled-coil domain-containing protein At3g58370 OS=Arabidopsis thaliana OX=3702 GN=At3g58370 PE=4 SV=3)

HSP 1 Score: 74.3 bits (181), Expect = 2.9e-12
Identity = 42/145 (28.97%), Postives = 74/145 (51.03%), Query Frame = 0

Query: 197 DYKFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEV 256
           D KFTW   NFS  + +   SDPF +   +W +  YP G+    +  LSLYL +D  + +
Sbjct: 7   DNKFTWVIKNFSTLQYDKIYSDPFVIGGCKWHLLAYPKGNKFNNS--LSLYLVVDDARAL 66

Query: 257 PQGFKVYVESEMAVLSQLGVGHRRYLTQKHWFEGEG-GRGWSEFMPLSNLKKPMKGFLHD 316
           P G++ Y +  + +++QL     +   +++WF     G G++  +PL NL     G+L +
Sbjct: 67  PCGWRRYAQFSLTIINQLTDKLSQRGEKQNWFNQRNLGSGFTSMIPLPNLHAKNAGYLVN 126

Query: 317 DTLMVEVKINVISTSKKIHRKENGD 341
             + + V+IN +    K+   E  +
Sbjct: 127 GEVKIVVEINDLEVIGKLDVSEESE 149

BLAST of Lag0001710 vs. ExPASy Swiss-Prot
Match: Q9M2H5 (MATH domain and coiled-coil domain-containing protein At3g58410 OS=Arabidopsis thaliana OX=3702 GN=At3g58410 PE=4 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 1.1e-11
Identity = 39/143 (27.27%), Postives = 74/143 (51.75%), Query Frame = 0

Query: 199 KFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQ 258
           KF W   NFS  +   + S PF + + +W++ +YP G++     +LSL+L +   K +P 
Sbjct: 8   KFAWVIKNFSSLQCKKFYSVPFQIGDCKWRLSIYPKGNN---CDYLSLFLEVADFKSLPS 67

Query: 259 GFKVYVESEMAVLSQLGVGHRRYLTQKHWFEGE-GGRGWSEFMPLSNLKKPMKGFLHDDT 318
           G++ YV+  + ++ QL    R+      WF+ E  G G+   +PL+ L    +GFL +  
Sbjct: 68  GWRRYVKLRLYIVKQLSTLIRK---THRWFDQEMWGWGFLYMLPLTKLHDEKEGFLVNGE 127

Query: 319 LMVEVKINVISTSKKIHRKENGD 341
           LM+  +++ +     ++  E  +
Sbjct: 128 LMIVAEVDALGFIDPLNESEESE 144

BLAST of Lag0001710 vs. ExPASy Swiss-Prot
Match: F4J4A1 (MATH domain and coiled-coil domain-containing protein At3g44800 OS=Arabidopsis thaliana OX=3702 GN=At3g44800 PE=4 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 8.0e-10
Identity = 41/136 (30.15%), Postives = 68/136 (50.00%), Query Frame = 0

Query: 199 KFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQ 258
           KFTW   NFS  +  Y +SD F +   +W +  YP G   A   +LSLYL   +LK +P 
Sbjct: 5   KFTWVIKNFSSLQSEYIKSDIFVIGGCKWCLLAYPNGKQNA--SYLSLYLDGPTLKTLPC 64

Query: 259 GFKVYVESEMAVLSQLGVGHRRYLTQKHWFEGEGGR-GWSEFMPLSNLKKPMKGFLHDDT 318
           G +  +   + V++QL     R    K WF+ +    G+ E + L+ L     GFL ++ 
Sbjct: 65  GCRRRIRFRLTVVNQLSENLSRRGEGKRWFDKKLPLCGYEEVLLLTKLNAKHGGFLVNNE 124

Query: 319 LMVEVKINVISTSKKI 334
           + +  +++V+    K+
Sbjct: 125 VKIVAEVDVLEVIGKL 138

BLAST of Lag0001710 vs. ExPASy TrEMBL
Match: A0A6J1CX47 (probable inactive serine/threonine-protein kinase fnkC OS=Momordica charantia OX=3673 GN=LOC111015608 PE=4 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 8.4e-95
Identity = 183/307 (59.61%), Postives = 219/307 (71.34%), Query Frame = 0

Query: 29  VRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKWKLALYPNGDDKR 88
           +RS+RD+KPLHYTLQIQSFSLLKAALASSN  RDRYESQ FN+GGYKWKLA+YPNGD KR
Sbjct: 9   IRSTRDEKPLHYTLQIQSFSLLKAALASSN--RDRYESQIFNAGGYKWKLAVYPNGDVKR 68

Query: 89  NGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGKVRRFHAIKKEWG 148
           NG+DHISLYLV A+DD+                             GKVRRFHA+K EWG
Sbjct: 69  NGADHISLYLVKADDDI------------------------NPCSHGKVRRFHAMKAEWG 128

Query: 149 FEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMKFEGNGEVLSFIKQPKDYKFTWKFTNFS 208
           FEKL+SLDTFND+S+G +V DCC FGVDV+VMK +G GEV+SFIK+PK +K+TWK  NFS
Sbjct: 129 FEKLISLDTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKTKNFS 188

Query: 209 LNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQGFKVYVESEM 268
                +YESDPFTVE YRWK+ LYP G S+A+TGFLSLYL  +S +E+PQG +VYVE E 
Sbjct: 189 QLDKKFYESDPFTVEGYRWKLWLYPKGSSKAKTGFLSLYLSYNSYEEIPQGSQVYVEYED 248

Query: 269 AVLSQLGVGHRRYLTQKHWFEGEGGRGWS--EFMPLSNLKKPMKGFLHDDTLMVEVKINV 328
           +     G G             E GRGW    F+ L +L +P +GF+ DDT++VEVKINV
Sbjct: 249 SHNCWFGFG-----------GVESGRGWGHPNFLSLRDLNEPTEGFIVDDTIIVEVKINV 278

Query: 329 ISTSKKI 334
           +ST KKI
Sbjct: 309 VSTLKKI 278

BLAST of Lag0001710 vs. ExPASy TrEMBL
Match: A0A6J1CZF7 (uncharacterized protein LOC111015610 OS=Momordica charantia OX=3673 GN=LOC111015610 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 1.0e-92
Identity = 189/335 (56.42%), Postives = 236/335 (70.45%), Query Frame = 0

Query: 4   KKRKTNDNFSPYPTPNPDSASTTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDR 63
           K+ + N   SP        A + S +RS RD+KPLHYTLQIQ FSLLK ALA+S+  RDR
Sbjct: 34  KRMQRNHIISP-----SSQADSGSVLRSIRDEKPLHYTLQIQPFSLLKTALATSS--RDR 93

Query: 64  YESQTFNSGGYKWKLALYPNGDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYD 123
           YES  F+  G+KWKLA+YP+GD KRNGS+HISLYLV AEDD+    T ++ V+FT LVYD
Sbjct: 94  YESPIFSVAGFKWKLAVYPSGDVKRNGSNHISLYLVTAEDDI---PTLDLTVVFTILVYD 153

Query: 124 HLKDKYLTVHDGKVRRFHAIKKEWGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMKFE 183
           +L+D YL V DGKVRRFHA+KKEWGFEKLVSL+TFND+S+G LVDDCCAFGVD+ VMK +
Sbjct: 154 NLRDNYLAVLDGKVRRFHAMKKEWGFEKLVSLETFNDSSNGFLVDDCCAFGVDIFVMKCD 213

Query: 184 GNGEVLSFIKQPKDYKFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGF 243
           G GE LS IKQPK+ K+TWK  NFS    N              K+ LYP G  +A   +
Sbjct: 214 GKGEALSLIKQPKNNKYTWKTNNFSQLDEN-------------MKMSLYPKGSPKASPDY 273

Query: 244 LSLYLRLDSLKEVPQGFKVYVESEMAVLSQLGVGHRRYLTQKHWFE-GEGGRGWSEFMPL 303
           LSL+LR +SLKE+PQG +VY E E+AVLS            K+WFE G+ G G+ +FM L
Sbjct: 274 LSLFLRCESLKELPQGCRVYAEFEIAVLS------------KYWFEFGDLGLGFPDFMSL 333

Query: 304 SNLKKPMKGFLHDDTLMVEVKINVISTSKKIHRKE 338
            +L +  KG++ DDTL+VEVKINV+ST K++ +KE
Sbjct: 334 RDLNEVTKGYICDDTLIVEVKINVVSTLKELFKKE 333

BLAST of Lag0001710 vs. ExPASy TrEMBL
Match: A0A6J1CXZ0 (ubiquitin carboxyl-terminal hydrolase 12-like OS=Momordica charantia OX=3673 GN=LOC111015607 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 3.0e-92
Identity = 195/323 (60.37%), Postives = 221/323 (68.42%), Query Frame = 0

Query: 21  DSASTTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKWKLAL 80
           D+ S    +RS+RD+KPLHYTLQIQSFSLLK AL  S+   DRYES+ FN+GGYKWKLA+
Sbjct: 16  DTDSGCVVIRSTRDEKPLHYTLQIQSFSLLKTALVRSD--CDRYESRIFNAGGYKWKLAM 75

Query: 81  YPNGDDKRNGS-DHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGKVRR 140
           YP GD KRNGS DHISLYLV AEDD+    TSEVNV+FTFLVYD LKDKYL V DG VRR
Sbjct: 76  YPTGDVKRNGSHDHISLYLVKAEDDI---PTSEVNVVFTFLVYDSLKDKYLAVQDGIVRR 135

Query: 141 FHAIKKEWGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMKFEGNGEVLSFIKQPKDYK 200
           FHAIK EWGFEKL+SLDTFND+S+G LVDDCCAFGVDV+VMK EG               
Sbjct: 136 FHAIKTEWGFEKLISLDTFNDSSNGFLVDDCCAFGVDVVVMKLEG--------------- 195

Query: 201 FTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQG 260
                                    YRWK+ LYP G S A TGFLSLYL LDS KE+ QG
Sbjct: 196 -------------------------YRWKLWLYPKGCSNAETGFLSLYLILDSSKEILQG 255

Query: 261 FKVYVESEMAVLSQLGVGHRRYLTQKHWFEGEGGRGWSEFMPLSNLKKPMKGFLHDDTLM 320
            KVYVE E+A+LSQLG    +  T  +WF G    G   F+ LS LKKP+KG+LH+DTL 
Sbjct: 256 SKVYVEYELALLSQLGAEPEKE-TYNYWFAG----GGPNFISLSELKKPLKGYLHNDTLK 288

Query: 321 VEVKINVISTSKKIH-RKENGDA 342
           VEVKINVISTSK  +  +EN  A
Sbjct: 316 VEVKINVISTSKSFNSSRENAAA 288

BLAST of Lag0001710 vs. ExPASy TrEMBL
Match: A0A6J1CZ69 (ubiquitin carboxyl-terminal hydrolase 13-like OS=Momordica charantia OX=3673 GN=LOC111015609 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 4.3e-91
Identity = 185/316 (58.54%), Postives = 222/316 (70.25%), Query Frame = 0

Query: 17  TPNPDSASTTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKW 76
           T +P S +  S +  +RD+KPLHYTLQIQSFSLLK ALA+SN   DRYESQ FN+GGYKW
Sbjct: 6   TISPSSEAADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASN--CDRYESQIFNAGGYKW 65

Query: 77  KLALYPNGDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGK 136
           KLA+YPNGD KRNGSDHISLYLV AED +    TSEVNV+FTFLVYD+L DKYL V D K
Sbjct: 66  KLAVYPNGDVKRNGSDHISLYLVKAEDGI---PTSEVNVVFTFLVYDNLNDKYLAVQDEK 125

Query: 137 VRRFHAIKKEWGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMKFEGNGEVLSFIKQPK 196
           VRRFH+I+ EW FEKL+SL+TFND+S+G LV DCCAFGVD++V K +G GEV+SFIK+PK
Sbjct: 126 VRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPK 185

Query: 197 DYKFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEV 256
           + ++TWK  NFS    N YESDPFTVE YR                           KE+
Sbjct: 186 NNEYTWKTNNFSQLDKNMYESDPFTVEGYR-------------------------CFKEL 245

Query: 257 PQGFKVYVESEMAVLSQLGVGHRRYLTQKHWFE-GEGGRGWSEFMPLSNLKKPMKGFLHD 316
           PQG +VY E E+AVLS            K+WFE G+ G G+  FM L +L +  KG++ D
Sbjct: 246 PQGSQVYTEFEIAVLS------------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICD 279

Query: 317 DTLMVEVKINVISTSK 332
           DTL+VEVKINV+ST K
Sbjct: 306 DTLIVEVKINVVSTLK 279

BLAST of Lag0001710 vs. ExPASy TrEMBL
Match: A0A1S3CQ20 (uncharacterized protein LOC103503506 OS=Cucumis melo OX=3656 GN=LOC103503506 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 2.8e-90
Identity = 187/328 (57.01%), Postives = 239/328 (72.87%), Query Frame = 0

Query: 17  TPNPDSASTTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKW 76
           +P+  ++++ SFV S+R +KP+HY L+IQSFSLLK A+ASS  Q  R+ESQ F +GG +W
Sbjct: 21  SPSTSASASASFVTSTRYEKPIHYILKIQSFSLLKEAVASSPCQ--RFESQKFYAGGSEW 80

Query: 77  KLALYPNGDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGK 136
           KLALYPNGD +R+ SDHISLYLV  E+++L +TTSEVNV+FTFLVYD L+ KYLTV D K
Sbjct: 81  KLALYPNGDRRRDVSDHISLYLVRVENNIL-STTSEVNVVFTFLVYDTLRGKYLTVQDEK 140

Query: 137 VRRFHAIKKEWGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMKFE-GNGEVLSFIKQP 196
           +R+F A K+EWG EKL+ L+TF+DAS+G LVDDCC FGVD+ VM      GEV S IKQP
Sbjct: 141 MRQFSATKREWGIEKLLPLNTFSDASNGFLVDDCCVFGVDIFVMNCNVEKGEVFSLIKQP 200

Query: 197 KDYKFTWKFTNFS-LNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLK 256
            +YK+TWK  NFS L   +++E + F VEN  WKI L+P+GD +A+ GF S+YL L +L 
Sbjct: 201 NNYKYTWKLDNFSKLLDCSFWECNSFRVENCWWKIRLFPSGDWQAKPGFFSMYLMLSNLI 260

Query: 257 EVPQGFKVYVESEMAVLSQLGVGHRRYLTQKHWF-----EGEGGRGWSEFMPLSNLKKPM 316
             P+G +VYVE EMAVLSQL        T   WF     E   G G S FM LS+LK+  
Sbjct: 261 AHPKGAQVYVEYEMAVLSQLEAVPPVKETYTCWFKSGCIEIYKGCGASNFMSLSDLKERT 320

Query: 317 KGFLHDDTLMVEVKINVISTSKKIHRKE 338
           KG+L +DTL+VEVKINVIST KK++R +
Sbjct: 321 KGYLVNDTLVVEVKINVISTIKKLYRSQ 345

BLAST of Lag0001710 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.7e-55
Identity = 117/308 (37.99%), Postives = 167/308 (54.22%), Query Frame = 0

Query: 22  SASTTSFVRSSRDQKPLHYTLQIQSFSLL-KAALASSNPQRDRYESQTFNSGGYKWKLAL 81
           S      ++S  D  P HY ++I+SFSLL K A+       +RYE+++F +GGYKWKL L
Sbjct: 4   SGDYDEIIKSISDAPPTHYMVKIESFSLLTKHAI-------ERYETESFEAGGYKWKLVL 63

Query: 82  YPNGDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGKVRRF 141
           YPNG+  +N  DH+S+YL +A+   L +   EV  +F   + D  KD YL +  G  RRF
Sbjct: 64  YPNGNKSKNTKDHVSVYLSLADSSSL-SPGWEVYAVFRLYLLDQNKDNYL-ILQGNERRF 123

Query: 142 HAIKKEWGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMK--FEGNGEVLSFIKQPKDY 201
           H++K+EWGF+K +   TF+DAS+G L++D C FG DV V K    G GE LS IK     
Sbjct: 124 HSVKREWGFDKFIPTGTFSDASNGYLMEDTCMFGADVFVSKERRSGRGECLSMIKDATSS 183

Query: 202 KFTWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQ 261
           K  WK  NFS      Y+S+ F   + +WKI  YPTG  +     LS+YL L   + +  
Sbjct: 184 KHVWKIENFSKLDKESYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISD 243

Query: 262 GFKVYVESEMAVLSQLGVGHRRYLTQKHWFEGEGGRGWSEFMPLSNLKKPMKGFLHDDTL 321
           G K++VE  + +  QL   H      K +       GW +++ +    +P  G L  D  
Sbjct: 244 GTKIFVEFTIRIFDQLQGRHIAGKVTKWFSRSSSEHGWVKYVSMVYFTQPNSGLLLKDVC 302

Query: 322 MVEVKINV 327
           +VE  + V
Sbjct: 304 LVEADVCV 302

BLAST of Lag0001710 vs. TAIR 10
Match: AT3G20360.1 (TRAF-like family protein )

HSP 1 Score: 188.3 bits (477), Expect = 9.9e-48
Identity = 116/314 (36.94%), Postives = 177/314 (56.37%), Query Frame = 0

Query: 24  STTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKWKLALYPN 83
           S +S V+  R++ P  Y+L+++SF+ L  ++ +     +RYES+ F  G Y W L +YP 
Sbjct: 53  SLSSTVKGLRERPPSSYSLKMESFNTLMKSVYT-----ERYESRPFRVGRYNWTLVVYPK 112

Query: 84  GDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGKVRRFHAI 143
           G+   NG+ HISLY+V+ ++  L + + EV+V   F V++  + KY T+ D  V RF AI
Sbjct: 113 GNKNDNGTGHISLYVVL-DNSTLTSQSEEVHVDLRFYVFNKKETKYFTIQDTDVWRFSAI 172

Query: 144 KKEWGFEKLVSLDTFNDASSGLLVD-DCCAFGVDVIVMKFEGNGEVLSFIKQPKDYKFTW 203
           K+ WGF K++ L TFN+  +G L D D C FGVDVI+  F    EV S  K     +FTW
Sbjct: 173 KRMWGFSKVLPLITFNNLKNGYLYDVDHCEFGVDVIIPPFYEKSEVFSVTKSFPSPRFTW 232

Query: 204 KFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRL---DSLKEVPQG 263
               +S    +Y  S+ F +    W + ++  G        LSLYL L   + LK  P  
Sbjct: 233 YIQGYSTLPTDYL-SEEFIIGGKSWNLRIFKNGFGAFEGKNLSLYLNLGPQELLKAKPYD 292

Query: 264 FKVYVESEMAVLSQLGVGHRRYLTQ--KHWFEGEG-GRGWSEFMPLSNLKKPMKGFLHDD 323
            KVYV +++ V +Q G      L +   +WF  +  G G+++FMPLS+L+   KGFL +D
Sbjct: 293 -KVYVRAKLRVPNQFGSQSNLVLERPLDNWFSPQTIGWGYADFMPLSDLRNSSKGFLVND 352

Query: 324 TLMVEVKINVISTS 331
            L+V+V +  IS++
Sbjct: 353 MLVVQVAMEEISST 358

BLAST of Lag0001710 vs. TAIR 10
Match: AT3G22080.1 (TRAF-like family protein )

HSP 1 Score: 179.1 bits (453), Expect = 6.0e-45
Identity = 97/311 (31.19%), Postives = 174/311 (55.95%), Query Frame = 0

Query: 22  SASTTSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKWKLALY 81
           S+ +++ +RS R++ P  Y+L++Q+ S ++ +   S+    +Y+S+ F+SGGY W++ +Y
Sbjct: 338 SSVSSTTLRSWRERTPNSYSLKLQNISQVEKSTLFSD---GKYQSRLFSSGGYNWRMIIY 397

Query: 82  PNGDDKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGKVRRFH 141
           P G+ K +GS  IS+Y+ +    +L T T+EV     F V++  ++KY T+   + + F+
Sbjct: 398 PKGNRKDDGSGFISMYVEIDSTSLLTTPTTEVFADLRFFVFNKKENKYYTIQHVESKLFN 457

Query: 142 AIKKEWGFEKLVSLDTFNDASSGLLVD-DCCAFGVDVIVMKFEGNGEVLSFIKQPKDYKF 201
           A +  WG  +++ +DTF D  +G + + D C FGVDVIV     N E+ +  +     KF
Sbjct: 458 AFRTIWGLAQVLPVDTFTDPKNGYIFEGDQCEFGVDVIVAAPPTNWEIHTLHEALSQPKF 517

Query: 202 TWKFTNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQGF 261
            W   NFS    N Y S  F++   +W + LYP GD +    +LSLYL LD  + + +  
Sbjct: 518 FWTVKNFSELNNNVYTSGNFSMRERKWVLKLYPKGDVKGDRKWLSLYLYLDQSETLKESE 577

Query: 262 KVYVESEMAVLSQLGVGHRRYLTQKHWFEGEGGRGWSEFMPLSNLKKPMKGFLHDDTLMV 321
           K++V++++ VL   G  H  +     +       G+ +F+ L+ +    K +L  DTL V
Sbjct: 578 KIFVQAQLRVLDPRGSNHVTHKISSWYTSSNTAWGYRKFVSLAEIP---KAYLDKDTLKV 637

Query: 322 EVKINVISTSK 332
           ++ + V+S ++
Sbjct: 638 QIDVEVVSEAE 642

BLAST of Lag0001710 vs. TAIR 10
Match: AT2G15710.1 (TRAF-like family protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.1e-43
Identity = 99/284 (34.86%), Postives = 154/284 (54.23%), Query Frame = 0

Query: 26  TSFVRSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKWKLALYPNGD 85
           ++ V + R+  P  Y+L+I   S L           D+YES  F SGGY W+L +YP G+
Sbjct: 87  STIVNNLREHPPSSYSLKINKLSQLTF---------DKYESHRFLSGGYNWRLVIYPKGN 146

Query: 86  DKRNGSDHISLYLVMAEDDVLWTTTSEVNVMFTFLVYDHLKDKYLTVHDGKVRRFHAIKK 145
           +K  GS  IS+Y+      V  T+  EV     F VY+  ++KY T+ D +V+RF+A++ 
Sbjct: 147 EKDKGSGFISMYVEFDNTKVSSTSPMEVFAYIIFFVYNKKENKYFTIQDVEVKRFNALRT 206

Query: 146 EWGFEKLVSLDTFNDASSGLLVD-DCCAFGVDVIVMKFEGNGEVLSFIKQPKDYKFTWKF 205
            WG  +++SL+TFND  +G   + + C FGVDV+V       EV+SF ++    KF+W  
Sbjct: 207 VWGLSQVLSLETFNDLENGYTFEGEQCEFGVDVMVASPITKWEVVSFDEKLDILKFSWSV 266

Query: 206 TNFSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQGFKVYV 265
            +FS+ K  +Y S+ F++    W + +YP GD R    +LS++LRL   + +    K+YV
Sbjct: 267 KDFSVLKEEFYVSERFSMGGRLWDLQMYPKGDPRRDKKWLSIFLRLSGSETLTVDEKIYV 326

Query: 266 ESEMAVLSQLGVGHRRYLTQKHWFEGEG-GRGWSEFMPLSNLKK 308
            + + VL  LG          +WF     G G+ EF+  S L+K
Sbjct: 327 IAHLRVLDPLG----------NWFRDRNKGWGYLEFLSFSKLRK 351

BLAST of Lag0001710 vs. TAIR 10
Match: AT5G26280.1 (TRAF-like family protein )

HSP 1 Score: 170.2 bits (430), Expect = 2.8e-42
Identity = 101/303 (33.33%), Postives = 157/303 (51.82%), Query Frame = 0

Query: 30  RSSRDQKPLHYTLQIQSFSLLKAALASSNPQRDRYESQTFNSGGYKWKLALYPNGDDKRN 89
           R  R+++P    + I SFS++K        + + YES  F + GYKW+L LY NG+    
Sbjct: 52  RELREERPSSKIVTITSFSVIKG-------RGEPYESSVFEAAGYKWRLVLYVNGNKNDG 111

Query: 90  GSDHISLYLVMAEDDVL---WTTTSEVNVMFTFLVYDHLKDKYLTVHDGKVRRFHAIKKE 149
           G+DHISLY  + E + L   W    EVNV     V++    KYLTV DG V+R++  KKE
Sbjct: 112 GNDHISLYARIEETNSLPLGW----EVNVDLKLFVHNGKLHKYLTVTDGLVKRYNNAKKE 171

Query: 150 WGFEKLVSLDTFNDASSGLLVDDCCAFGVDVIVMKFEGNGEVLSFIKQPKDYKFTWKFTN 209
           WGF +L+   TF +A+ G L  D  +FG ++ ++K     E ++FI  P +  FTWK   
Sbjct: 172 WGFGQLIPRSTFYNANEGYLDQDTGSFGAEIFIVKPAQQQEKVTFISNPPNNVFTWKILR 231

Query: 210 FSLNKLNYYESDPFTVENYRWKIGLYPTGDSRARTGFLSLYLRLDSLKEVPQGFKVYVES 269
           FS  +  +Y SD F VE+  W++G  P GD   R   L ++L     K        +   
Sbjct: 232 FSTLEDKFYYSDDFLVEDRYWRLGFNPKGDGGGRPHALPIFLFAQGHKANAVATNTWGAV 291

Query: 270 EMAVLSQLGVGHRRYLTQKHWFEGEG-GRGWSEFMPLSNLKKPMKGFLHDDTLMVEVKIN 329
            + + +Q    HR+  +   +  G G G G +  + L++L    KG+L +D ++ E ++ 
Sbjct: 292 NLRLKNQRSTNHRQIYSAAWYPIGSGYGVGVNNIILLADLNDASKGYLVNDAIIFEAEMV 343