Lag0001676 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0001676
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionadenylate isopentenyltransferase 5, chloroplastic-like
Locationchr4: 34211447 .. 34212445 (-)
RNA-Seq ExpressionLag0001676
SyntenyLag0001676
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGACAGCGTTGAGTATCTGAGCAACAAGAAGCACAAAATCGTGTTCGTTATGGGCTGCACTGCTACTGGAAAATCAAAACTCTCCGTCGATTTGGCTACTTTCTTCCCGTCAGAGATCATCAACTCCGACAAGATTCAGTTCTACAAAGGCCTCGACATCATCACCAACAAGATCACCGATTCCGATCGTCGCGGCGTTCCTCACCATCTCCTCGGCGTCATCGAGGATCCGGACGCAGATTTGACGGCCGGAGAGTTCTGCCGCCTCGTCGAAGGGTCGATCGCAGAGATTATCGGACGCGGACGGCTTCCGATCGTCGTCGGAGGATCGAACAACTACATCGAGGCACTGGTGGAGAATCCGATTACGGATTTCCGTTCGCGATTCGACTGTTGTTTTGTTTGGATCGACGTTTCGATTCCGGTTCTGTACAGGTACATCGCGAAGCGAGTCGATCAGATGGTGGAGGCAGGGCTAGTGGAGGAGGTTCGAGAGATGTTCGTTCCTGGCGCCGACTACAGCCGCGGAATCCGCCGCGCGATCGGCGCTCCGGAGATGGACAGTTACTTCAAAGCGGAGAGAAACGACGAAGAATCGTACAAGGACGATTTGCTGAAATCGGCGATTCAAGAAATCAAGGACAATACCTGCAAGCTCGCGTTCCGTCAATTCGGAAAAATTCATCGGCTCCGAGACGAGCTCGGTTGGGGACTCCACCGGATCGACGCAACCGCGGTGTTCGAGAAGAGCGGAGAAGACGCCGCCGATGAGTGGATGAATGCGGTTCTGAAACCGAGCTTGGCGATCGTCGGCGATTTCCTCAACGAAAAGCATGAGGAGAAAACTTCCAAAAGAATCAAGAACCGATTCGCGAGAAACGGTGGTTTCGAAGAACTCAAAACGAAGTTCAATCTCGGGTCCAACGCGATCGGATTCGGCCTTGTGTTCGCATTCGCGGCTTTAGGTTTGGTAACGAACTGGATTTGCTGTTGA

mRNA sequence

ATGAAGGACAGCGTTGAGTATCTGAGCAACAAGAAGCACAAAATCGTGTTCGTTATGGGCTGCACTGCTACTGGAAAATCAAAACTCTCCGTCGATTTGGCTACTTTCTTCCCGTCAGAGATCATCAACTCCGACAAGATTCAGTTCTACAAAGGCCTCGACATCATCACCAACAAGATCACCGATTCCGATCGTCGCGGCGTTCCTCACCATCTCCTCGGCGTCATCGAGGATCCGGACGCAGATTTGACGGCCGGAGAGTTCTGCCGCCTCGTCGAAGGGTCGATCGCAGAGATTATCGGACGCGGACGGCTTCCGATCGTCGTCGGAGGATCGAACAACTACATCGAGGCACTGGTGGAGAATCCGATTACGGATTTCCGTTCGCGATTCGACTGTTGTTTTGTTTGGATCGACGTTTCGATTCCGGTTCTGTACAGGTACATCGCGAAGCGAGTCGATCAGATGGTGGAGGCAGGGCTAGTGGAGGAGGTTCGAGAGATGTTCGTTCCTGGCGCCGACTACAGCCGCGGAATCCGCCGCGCGATCGGCGCTCCGGAGATGGACAGTTACTTCAAAGCGGAGAGAAACGACGAAGAATCGTACAAGGACGATTTGCTGAAATCGGCGATTCAAGAAATCAAGGACAATACCTGCAAGCTCGCGTTCCGTCAATTCGGAAAAATTCATCGGCTCCGAGACGAGCTCGGTTGGGGACTCCACCGGATCGACGCAACCGCGGTGTTCGAGAAGAGCGGAGAAGACGCCGCCGATGAGTGGATGAATGCGGTTCTGAAACCGAGCTTGGCGATCGTCGGCGATTTCCTCAACGAAAAGCATGAGGAGAAAACTTCCAAAAGAATCAAGAACCGATTCGCGAGAAACGGTGGTTTCGAAGAACTCAAAACGAAGTTCAATCTCGGGTCCAACGCGATCGGATTCGGCCTTGTGTTCGCATTCGCGGCTTTAGGTTTGGTAACGAACTGGATTTGCTGTTGA

Coding sequence (CDS)

ATGAAGGACAGCGTTGAGTATCTGAGCAACAAGAAGCACAAAATCGTGTTCGTTATGGGCTGCACTGCTACTGGAAAATCAAAACTCTCCGTCGATTTGGCTACTTTCTTCCCGTCAGAGATCATCAACTCCGACAAGATTCAGTTCTACAAAGGCCTCGACATCATCACCAACAAGATCACCGATTCCGATCGTCGCGGCGTTCCTCACCATCTCCTCGGCGTCATCGAGGATCCGGACGCAGATTTGACGGCCGGAGAGTTCTGCCGCCTCGTCGAAGGGTCGATCGCAGAGATTATCGGACGCGGACGGCTTCCGATCGTCGTCGGAGGATCGAACAACTACATCGAGGCACTGGTGGAGAATCCGATTACGGATTTCCGTTCGCGATTCGACTGTTGTTTTGTTTGGATCGACGTTTCGATTCCGGTTCTGTACAGGTACATCGCGAAGCGAGTCGATCAGATGGTGGAGGCAGGGCTAGTGGAGGAGGTTCGAGAGATGTTCGTTCCTGGCGCCGACTACAGCCGCGGAATCCGCCGCGCGATCGGCGCTCCGGAGATGGACAGTTACTTCAAAGCGGAGAGAAACGACGAAGAATCGTACAAGGACGATTTGCTGAAATCGGCGATTCAAGAAATCAAGGACAATACCTGCAAGCTCGCGTTCCGTCAATTCGGAAAAATTCATCGGCTCCGAGACGAGCTCGGTTGGGGACTCCACCGGATCGACGCAACCGCGGTGTTCGAGAAGAGCGGAGAAGACGCCGCCGATGAGTGGATGAATGCGGTTCTGAAACCGAGCTTGGCGATCGTCGGCGATTTCCTCAACGAAAAGCATGAGGAGAAAACTTCCAAAAGAATCAAGAACCGATTCGCGAGAAACGGTGGTTTCGAAGAACTCAAAACGAAGTTCAATCTCGGGTCCAACGCGATCGGATTCGGCCTTGTGTTCGCATTCGCGGCTTTAGGTTTGGTAACGAACTGGATTTGCTGTTGA

Protein sequence

MKDSVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKITDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGIRRAIGAPEMDSYFKAERNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHEEKTSKRIKNRFARNGGFEELKTKFNLGSNAIGFGLVFAFAALGLVTNWICC
Homology
BLAST of Lag0001676 vs. NCBI nr
Match: XP_038888774.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 570.9 bits (1470), Expect = 7.4e-159
Identity = 282/334 (84.43%), Postives = 306/334 (91.62%), Query Frame = 0

Query: 1   MKDSVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKI 60
           MKDSVEYLSNKKHKIVFVMG TATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKI
Sbjct: 1   MKDSVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKI 60

Query: 61  TDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALV 120
           T+SDRRGVPHHLLGVI+DPDADLTAGEFCRLVE SIA+II  GRLPI+VGGSNNYIEALV
Sbjct: 61  TESDRRGVPHHLLGVIDDPDADLTAGEFCRLVEDSIADIISHGRLPIIVGGSNNYIEALV 120

Query: 121 ENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGIR 180
           ENPITDFRSRFDCCF+WIDV++PVLY+YI KRVDQMVE GLVEEVR+MFVPGADYSRGIR
Sbjct: 121 ENPITDFRSRFDCCFLWIDVALPVLYKYIGKRVDQMVELGLVEEVRDMFVPGADYSRGIR 180

Query: 181 RAIGAPEMDSYFKAER-NDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWG 240
           RAIGAPE+DSYFKAE+ N+EESYK+DLLKS I EIK+NTCKLA RQFGKIHRLRDELGWG
Sbjct: 181 RAIGAPELDSYFKAEKNNEEESYKNDLLKSGIHEIKENTCKLALRQFGKIHRLRDELGWG 240

Query: 241 LHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHEE-KTSKRIKNRFARNGGF 300
           LHR+DATAVFEKSGE+AADEWMNAVLKPSL IVGDFLNEK ++ +    IKNR A   G+
Sbjct: 241 LHRVDATAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQKDYQIEAVIKNRLAIMNGY 300

Query: 301 EELKTKFNLGS-NAIGFGLVFAFAALGLVTNWIC 332
           EELK K N GS NA+G+GLV  FAA GL+TNW+C
Sbjct: 301 EELKAKMNFGSNNAVGYGLVIGFAAFGLITNWVC 334

BLAST of Lag0001676 vs. NCBI nr
Match: KAA0036515.1 (adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK13465.1 adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 538.9 bits (1387), Expect = 3.1e-149
Identity = 274/335 (81.79%), Postives = 295/335 (88.06%), Query Frame = 0

Query: 1   MKDS-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKDS VEY SNKKHKIVFV+G TATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEAL 120
           I  SDRRGVPHHLLGVI +PDADLTAGEFCRLVE SIA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCF+W DV++PVLY+YI KRVDQMVE GLVEEVREMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFKAER-NDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGW 240
           RRAIGAPE++SYFKAE+ N+EE+YK +LLKSAI EIK+NTCKLA RQFGKIHRLRDELGW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHEE-KTSKRIKNRF-ARNG 300
           GLHRID TAVFEKSGE+AADEWMNAVLKPSL IVGDFLNEK    +     KNR    N 
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNRMGVMNN 300

Query: 301 GFEELKTKFNLGS-NAIGFGLVFAFAALGLVTNWI 331
           G+EELK K+N GS NAIGFGLV  F +L L+ NWI
Sbjct: 301 GYEELKGKWNFGSNNAIGFGLVIGFTSLSLMANWI 335

BLAST of Lag0001676 vs. NCBI nr
Match: XP_004148309.1 (adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus])

HSP 1 Score: 537.7 bits (1384), Expect = 6.9e-149
Identity = 273/335 (81.49%), Postives = 297/335 (88.66%), Query Frame = 0

Query: 1   MKDS-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKD+ VEYLSNKKHKIVFVMG TATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEAL 120
           I  SDRRGVPHHLLGVI DPDADLTAGEFC LVE +IA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCF+W DV++PVLY+YIAKRVDQMVE GLVEEVREMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFKAER-NDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGW 240
           RRAIGAPE+DSYFKAE+ N+EE+YK+DLLKS I EIK+NTC+LA RQFGKIHRLRDE+GW
Sbjct: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHE-EKTSKRIKNR-FARNG 300
           GLHRIDATAVFEKSGE+AADEWMN VLKPSL IVG+FLNEK +  +     KNR      
Sbjct: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300

Query: 301 GFEELKTKFNLGS-NAIGFGLVFAFAALGLVTNWI 331
            +EELK K+N GS NAIGFGLV  F +LGL+TNWI
Sbjct: 301 EYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWI 335

BLAST of Lag0001676 vs. NCBI nr
Match: XP_008447303.1 (PREDICTED: adenylate isopentenyltransferase 5, chloroplastic-like [Cucumis melo])

HSP 1 Score: 537.0 bits (1382), Expect = 1.2e-148
Identity = 273/335 (81.49%), Postives = 294/335 (87.76%), Query Frame = 0

Query: 1   MKDS-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKDS VEY SNKKHKIVFV+G TATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEAL 120
           I  SDRRGVPHHLLGVI +PDADLTAGEFCRLVE SIA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCF+W DV++PVLY+YI KRVDQMVE GLVEEV EMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVGEMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFKAER-NDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGW 240
           RRAIGAPE++SYFKAE+ N+EE+YK +LLKSAI EIK+NTCKLA RQFGKIHRLRDELGW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHEE-KTSKRIKNRF-ARNG 300
           GLHRID TAVFEKSGE+AADEWMNAVLKPSL IVGDFLNEK    +     KNR    N 
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNRMGVMNN 300

Query: 301 GFEELKTKFNLGS-NAIGFGLVFAFAALGLVTNWI 331
           G+EELK K+N GS NAIGFGLV  F +L L+ NWI
Sbjct: 301 GYEELKAKWNFGSNNAIGFGLVIGFTSLSLMANWI 335

BLAST of Lag0001676 vs. NCBI nr
Match: XP_022972357.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 503.1 bits (1294), Expect = 1.9e-138
Identity = 248/305 (81.31%), Postives = 270/305 (88.52%), Query Frame = 0

Query: 1   MKDSVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKI 60
           M +SVEYLSNKKHK++FVMGCTATGKSKLSVDLATFFPSEIINSDKIQFY+GLDIITNKI
Sbjct: 1   MNNSVEYLSNKKHKVIFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYEGLDIITNKI 60

Query: 61  TDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALV 120
            +SDRRGVPHHLLGVI+DPDAD TA EFCRLVE SIAEIIGRGR+PI+VGGSNNYIEALV
Sbjct: 61  AESDRRGVPHHLLGVIQDPDADFTAAEFCRLVEDSIAEIIGRGRIPIIVGGSNNYIEALV 120

Query: 121 ENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGIR 180
           ENPI DFRSRFDCCFVW DV++PVL RYI+KRVDQMVEAGLVEEVREM+VP ADYSRGIR
Sbjct: 121 ENPIADFRSRFDCCFVWTDVALPVLNRYISKRVDQMVEAGLVEEVREMYVPNADYSRGIR 180

Query: 181 RAIGAPEMDSYFKAERNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGL 240
           RAIGAPEMDSYFK+E+ND     D LLKSAIQEIKDNTC LA RQ GKIHRLRDELGW +
Sbjct: 181 RAIGAPEMDSYFKSEKND-----DSLLKSAIQEIKDNTCTLALRQRGKIHRLRDELGWRI 240

Query: 241 HRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHEE-KTSKRIKNRFARNGGFE 300
           HRIDATAVFEKSGED AD WMN VLKPSL IVG+FLN++H++ +     KN F  N G+ 
Sbjct: 241 HRIDATAVFEKSGEDTADVWMNTVLKPSLDIVGEFLNKEHKDYRIETMSKNGFTTNSGYG 300

Query: 301 ELKTK 305
           ELK K
Sbjct: 301 ELKAK 300

BLAST of Lag0001676 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 296.6 bits (758), Expect = 3.5e-79
Identity = 146/269 (54.28%), Postives = 196/269 (72.86%), Query Frame = 0

Query: 11  KKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKITDSDRRGVPH 70
           +K K+VFVMG T TGKS+L++DLAT FP+EI+NSDKIQ YKGLDI+TNK+T  +  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 71  HLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSR 130
           HLLG + D   D TA +F R    ++  I+ R R+PI+ GGSN+YIEALV N   DFR R
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV-NDCVDFRLR 150

Query: 131 FDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVP-GADYSRGIRRAIGAPEMD 190
           ++CCF+W+DVS PVL+ ++++RVD+MV+ GLV+EVR +F P  +DYS GIRRAIG PE+D
Sbjct: 151 YNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELD 210

Query: 191 SYFKAE-RNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGLHRIDATAV 250
            + ++E RN      + LL++AI++IK+NTC LA RQ  KI RL  +  W +HR+DAT V
Sbjct: 211 EFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEV 270

Query: 251 FEKSGEDAADEWMNAVLKPSLAIVGDFLN 278
           F + GE+A + W N+V  PS   V  FL+
Sbjct: 271 FLRRGEEADEAWDNSVAHPSALAVEKFLS 298

BLAST of Lag0001676 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.7e-76
Identity = 144/268 (53.73%), Postives = 192/268 (71.64%), Query Frame = 0

Query: 12  KHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKITDSDRRGVPHH 71
           K K+V +MG T TGKS+LSVD+AT F +EIINSDKIQ ++GLDI+TNKIT  +  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 72  LLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSRF 131
           LLGV+  P+ADLTA  +C +   SI  ++ RG+LPI+VGGSN+Y+EALV++    FRSR+
Sbjct: 100 LLGVL-PPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRY 159

Query: 132 DCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMF-VPGADYSRGIRRAIGAPEMDS 191
           DCCF+W+DV++PVL+ ++++RVD+MVE+G+VEEVRE F    +DYSRGI++AIG PE D 
Sbjct: 160 DCCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDR 219

Query: 192 YFKAERNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGLHRIDATAVFE 251
           +F+ E+      +++LL   ++EIK NT +LA RQ  KI RLR    W + R+DAT VF 
Sbjct: 220 FFRNEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFT 279

Query: 252 K--SGEDAADEWMNAVLKPSLAIVGDFL 277
           K  S  DA   W   V  PS   V  FL
Sbjct: 280 KRRSKMDANVAWERLVAGPSTDTVSRFL 306

BLAST of Lag0001676 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 282.0 bits (720), Expect = 9.0e-75
Identity = 142/283 (50.18%), Postives = 192/283 (67.84%), Query Frame = 0

Query: 2   KDSVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKIT 61
           K +V    + K K++FVMG T +GKS+L++DLAT F  EIINSDKIQ YKGLD++TNK+T
Sbjct: 23  KVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVT 82

Query: 62  DSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALVE 121
             + RGVPHHLLGV +    +LTA ++ RL   +I+++    +LPIV GGSN+YIEALV 
Sbjct: 83  PKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVN 142

Query: 122 NPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGIRR 181
           +      + +DCCF+W+DVS+PVL  +++KRVD+M+EAGL+EEVRE+F P A+YS GIRR
Sbjct: 143 HSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRR 202

Query: 182 AIGAPEMDSYFKAERNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGLH 241
           AIG PE+  Y + E   + + K  +L  A++ IK NT  LA RQ  KI RL  +    +H
Sbjct: 203 AIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWKMSMH 262

Query: 242 RIDATAVFEKSGEDAADE-WMNAVLKPSLAIVGDFLNEKHEEK 284
           R+DAT VF K   +  DE W N V +PS  IV  F N  ++ K
Sbjct: 263 RVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNNNQLK 305

BLAST of Lag0001676 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-63
Identity = 135/304 (44.41%), Postives = 187/304 (61.51%), Query Frame = 0

Query: 10  NKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKITDSDRRGVP 69
           ++K K++ +MG T TGKS+LS+DLA  FP E+INSDK+Q YKGLDI TNKI+  DR GVP
Sbjct: 27  HRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVP 86

Query: 70  HHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALV--------- 129
           HHLLG ++    +LT  +F  L   +++EI GR +LP++VGGSN++I AL+         
Sbjct: 87  HHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGP 146

Query: 130 ------ENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVP-GA 189
                  + +     R+DCCF+W+DVS+ VL  Y+AKRVD M+E G+ +E+ E + P   
Sbjct: 147 GVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDE 206

Query: 190 DYSR------GIRRAIGAPEMDSYFKAER----NDEESYKDDLLKSAIQE----IKDNTC 249
           D+        G+R+AIG PE D YF+  R      E+  +D + + A +E    IK+NTC
Sbjct: 207 DHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTC 266

Query: 250 KLAFRQFGKIHRLRDELGWGLHRIDAT-----AVFEKSGEDAADEWMNAVLKPSLAIVGD 279
            LA RQ GKI RL+   GW L R+DAT     A+   SGE   + W   VL+PS+ IV  
Sbjct: 267 HLAKRQIGKILRLKG-AGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSR 326

BLAST of Lag0001676 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 241.9 bits (616), Expect = 1.0e-62
Identity = 133/290 (45.86%), Postives = 180/290 (62.07%), Query Frame = 0

Query: 10  NKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKITDSDRRGVP 69
           N+K K+V ++G T  GKS+LSVDLAT FPSEIINSDKIQ Y+GL+I TN+IT  DRRGVP
Sbjct: 62  NRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVP 121

Query: 70  HHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALV----ENPIT 129
           HHLLGVI     +LTAGEF       + EI  R ++PI+ GGSN+++ AL+    +    
Sbjct: 122 HHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 181

Query: 130 DFRS---------RFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVP---GA 189
            F S         R++CCF+W+DVS  VLY Y+ +RVD+M+++G+ EE+   + P   G 
Sbjct: 182 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 241

Query: 190 DYSRGIRRAIGAPEMDSYFKAERNDEESYKDDLLK-----SAIQEIKDNTCKLAFRQFGK 249
           +   GIR+AIG PE D YFK    +++  K D L+      A+ +IK NT  LA RQ  K
Sbjct: 242 ETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKK 301

Query: 250 IHRLRDELGWGLHRIDATAVFE------KSGEDAADEWMNAVLKPSLAIV 273
           I  L+D  GW + R+DATA F+       S +   + W   VL+PS+ IV
Sbjct: 302 IEMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIV 350

BLAST of Lag0001676 vs. ExPASy TrEMBL
Match: A0A5D3CPK4 (Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold179G00270 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.5e-149
Identity = 274/335 (81.79%), Postives = 295/335 (88.06%), Query Frame = 0

Query: 1   MKDS-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKDS VEY SNKKHKIVFV+G TATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEAL 120
           I  SDRRGVPHHLLGVI +PDADLTAGEFCRLVE SIA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCF+W DV++PVLY+YI KRVDQMVE GLVEEVREMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFKAER-NDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGW 240
           RRAIGAPE++SYFKAE+ N+EE+YK +LLKSAI EIK+NTCKLA RQFGKIHRLRDELGW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHEE-KTSKRIKNRF-ARNG 300
           GLHRID TAVFEKSGE+AADEWMNAVLKPSL IVGDFLNEK    +     KNR    N 
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNRMGVMNN 300

Query: 301 GFEELKTKFNLGS-NAIGFGLVFAFAALGLVTNWI 331
           G+EELK K+N GS NAIGFGLV  F +L L+ NWI
Sbjct: 301 GYEELKGKWNFGSNNAIGFGLVIGFTSLSLMANWI 335

BLAST of Lag0001676 vs. ExPASy TrEMBL
Match: A0A0A0K7L1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G253720 PE=4 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.4e-149
Identity = 273/335 (81.49%), Postives = 297/335 (88.66%), Query Frame = 0

Query: 1   MKDS-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKD+ VEYLSNKKHKIVFVMG TATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEAL 120
           I  SDRRGVPHHLLGVI DPDADLTAGEFC LVE +IA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCF+W DV++PVLY+YIAKRVDQMVE GLVEEVREMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFKAER-NDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGW 240
           RRAIGAPE+DSYFKAE+ N+EE+YK+DLLKS I EIK+NTC+LA RQFGKIHRLRDE+GW
Sbjct: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHE-EKTSKRIKNR-FARNG 300
           GLHRIDATAVFEKSGE+AADEWMN VLKPSL IVG+FLNEK +  +     KNR      
Sbjct: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300

Query: 301 GFEELKTKFNLGS-NAIGFGLVFAFAALGLVTNWI 331
            +EELK K+N GS NAIGFGLV  F +LGL+TNWI
Sbjct: 301 EYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWI 335

BLAST of Lag0001676 vs. ExPASy TrEMBL
Match: A0A1S3BH37 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489774 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 5.7e-149
Identity = 273/335 (81.49%), Postives = 294/335 (87.76%), Query Frame = 0

Query: 1   MKDS-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKDS VEY SNKKHKIVFV+G TATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEAL 120
           I  SDRRGVPHHLLGVI +PDADLTAGEFCRLVE SIA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCF+W DV++PVLY+YI KRVDQMVE GLVEEV EMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVGEMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFKAER-NDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGW 240
           RRAIGAPE++SYFKAE+ N+EE+YK +LLKSAI EIK+NTCKLA RQFGKIHRLRDELGW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHEE-KTSKRIKNRF-ARNG 300
           GLHRID TAVFEKSGE+AADEWMNAVLKPSL IVGDFLNEK    +     KNR    N 
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNRMGVMNN 300

Query: 301 GFEELKTKFNLGS-NAIGFGLVFAFAALGLVTNWI 331
           G+EELK K+N GS NAIGFGLV  F +L L+ NWI
Sbjct: 301 GYEELKAKWNFGSNNAIGFGLVIGFTSLSLMANWI 335

BLAST of Lag0001676 vs. ExPASy TrEMBL
Match: A0A6J1I4L3 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111470933 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 9.2e-139
Identity = 248/305 (81.31%), Postives = 270/305 (88.52%), Query Frame = 0

Query: 1   MKDSVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKI 60
           M +SVEYLSNKKHK++FVMGCTATGKSKLSVDLATFFPSEIINSDKIQFY+GLDIITNKI
Sbjct: 1   MNNSVEYLSNKKHKVIFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYEGLDIITNKI 60

Query: 61  TDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALV 120
            +SDRRGVPHHLLGVI+DPDAD TA EFCRLVE SIAEIIGRGR+PI+VGGSNNYIEALV
Sbjct: 61  AESDRRGVPHHLLGVIQDPDADFTAAEFCRLVEDSIAEIIGRGRIPIIVGGSNNYIEALV 120

Query: 121 ENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGIR 180
           ENPI DFRSRFDCCFVW DV++PVL RYI+KRVDQMVEAGLVEEVREM+VP ADYSRGIR
Sbjct: 121 ENPIADFRSRFDCCFVWTDVALPVLNRYISKRVDQMVEAGLVEEVREMYVPNADYSRGIR 180

Query: 181 RAIGAPEMDSYFKAERNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGL 240
           RAIGAPEMDSYFK+E+ND     D LLKSAIQEIKDNTC LA RQ GKIHRLRDELGW +
Sbjct: 181 RAIGAPEMDSYFKSEKND-----DSLLKSAIQEIKDNTCTLALRQRGKIHRLRDELGWRI 240

Query: 241 HRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHEE-KTSKRIKNRFARNGGFE 300
           HRIDATAVFEKSGED AD WMN VLKPSL IVG+FLN++H++ +     KN F  N G+ 
Sbjct: 241 HRIDATAVFEKSGEDTADVWMNTVLKPSLDIVGEFLNKEHKDYRIETMSKNGFTTNSGYG 300

Query: 301 ELKTK 305
           ELK K
Sbjct: 301 ELKAK 300

BLAST of Lag0001676 vs. ExPASy TrEMBL
Match: A0A6J1GLR3 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111455496 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.9e-138
Identity = 247/305 (80.98%), Postives = 267/305 (87.54%), Query Frame = 0

Query: 1   MKDSVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKI 60
           M  SVEYLSNKKHK++FVMGCTATGKSKLSVDLA FFPSEIINSDKIQFY+GLDIITNKI
Sbjct: 4   MNHSVEYLSNKKHKVIFVMGCTATGKSKLSVDLANFFPSEIINSDKIQFYEGLDIITNKI 63

Query: 61  TDSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALV 120
            +SDRRGVPHHLLGVI+DPDADLTA EFCRLVE SIAEIIGRGR+PI+VGGSNNYIEALV
Sbjct: 64  VESDRRGVPHHLLGVIQDPDADLTAAEFCRLVEDSIAEIIGRGRIPIIVGGSNNYIEALV 123

Query: 121 ENPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGIR 180
           ENPI DFRSRFDCCFVW DV++PVL RYI+KRVDQMVEAGLVEEVREM+VP ADYSRGIR
Sbjct: 124 ENPIADFRSRFDCCFVWTDVALPVLNRYISKRVDQMVEAGLVEEVREMYVPNADYSRGIR 183

Query: 181 RAIGAPEMDSYFKAERNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGL 240
           RAIGAPEMDSYFK+E+ND     D LLKSAIQEIKDNTC LA RQ GKIHRLRDELGWG+
Sbjct: 184 RAIGAPEMDSYFKSEKND-----DSLLKSAIQEIKDNTCTLALRQRGKIHRLRDELGWGI 243

Query: 241 HRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHEE-KTSKRIKNRFARNGGFE 300
           HRIDATAVFE SGED AD WMN VLKPSL IVG FLN++H++ +     KN F  N  + 
Sbjct: 244 HRIDATAVFENSGEDTADVWMNTVLKPSLEIVGKFLNKEHKDYRIETMTKNGFTTNSSYG 303

Query: 301 ELKTK 305
           ELK K
Sbjct: 304 ELKAK 303

BLAST of Lag0001676 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 296.6 bits (758), Expect = 2.5e-80
Identity = 146/269 (54.28%), Postives = 196/269 (72.86%), Query Frame = 0

Query: 11  KKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKITDSDRRGVPH 70
           +K K+VFVMG T TGKS+L++DLAT FP+EI+NSDKIQ YKGLDI+TNK+T  +  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 71  HLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSR 130
           HLLG + D   D TA +F R    ++  I+ R R+PI+ GGSN+YIEALV N   DFR R
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV-NDCVDFRLR 150

Query: 131 FDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVP-GADYSRGIRRAIGAPEMD 190
           ++CCF+W+DVS PVL+ ++++RVD+MV+ GLV+EVR +F P  +DYS GIRRAIG PE+D
Sbjct: 151 YNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELD 210

Query: 191 SYFKAE-RNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGLHRIDATAV 250
            + ++E RN      + LL++AI++IK+NTC LA RQ  KI RL  +  W +HR+DAT V
Sbjct: 211 EFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEV 270

Query: 251 FEKSGEDAADEWMNAVLKPSLAIVGDFLN 278
           F + GE+A + W N+V  PS   V  FL+
Sbjct: 271 FLRRGEEADEAWDNSVAHPSALAVEKFLS 298

BLAST of Lag0001676 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 286.6 bits (732), Expect = 2.6e-77
Identity = 144/268 (53.73%), Postives = 192/268 (71.64%), Query Frame = 0

Query: 12  KHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKITDSDRRGVPHH 71
           K K+V +MG T TGKS+LSVD+AT F +EIINSDKIQ ++GLDI+TNKIT  +  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 72  LLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSRF 131
           LLGV+  P+ADLTA  +C +   SI  ++ RG+LPI+VGGSN+Y+EALV++    FRSR+
Sbjct: 100 LLGVL-PPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRY 159

Query: 132 DCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMF-VPGADYSRGIRRAIGAPEMDS 191
           DCCF+W+DV++PVL+ ++++RVD+MVE+G+VEEVRE F    +DYSRGI++AIG PE D 
Sbjct: 160 DCCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDR 219

Query: 192 YFKAERNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGLHRIDATAVFE 251
           +F+ E+      +++LL   ++EIK NT +LA RQ  KI RLR    W + R+DAT VF 
Sbjct: 220 FFRNEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFT 279

Query: 252 K--SGEDAADEWMNAVLKPSLAIVGDFL 277
           K  S  DA   W   V  PS   V  FL
Sbjct: 280 KRRSKMDANVAWERLVAGPSTDTVSRFL 306

BLAST of Lag0001676 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 282.0 bits (720), Expect = 6.4e-76
Identity = 142/283 (50.18%), Postives = 192/283 (67.84%), Query Frame = 0

Query: 2   KDSVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKIT 61
           K +V    + K K++FVMG T +GKS+L++DLAT F  EIINSDKIQ YKGLD++TNK+T
Sbjct: 23  KVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVT 82

Query: 62  DSDRRGVPHHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALVE 121
             + RGVPHHLLGV +    +LTA ++ RL   +I+++    +LPIV GGSN+YIEALV 
Sbjct: 83  PKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVN 142

Query: 122 NPITDFRSRFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVPGADYSRGIRR 181
           +      + +DCCF+W+DVS+PVL  +++KRVD+M+EAGL+EEVRE+F P A+YS GIRR
Sbjct: 143 HSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRR 202

Query: 182 AIGAPEMDSYFKAERNDEESYKDDLLKSAIQEIKDNTCKLAFRQFGKIHRLRDELGWGLH 241
           AIG PE+  Y + E   + + K  +L  A++ IK NT  LA RQ  KI RL  +    +H
Sbjct: 203 AIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWKMSMH 262

Query: 242 RIDATAVFEKSGEDAADE-WMNAVLKPSLAIVGDFLNEKHEEK 284
           R+DAT VF K   +  DE W N V +PS  IV  F N  ++ K
Sbjct: 263 RVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNNNQLK 305

BLAST of Lag0001676 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 241.9 bits (616), Expect = 7.3e-64
Identity = 133/290 (45.86%), Postives = 180/290 (62.07%), Query Frame = 0

Query: 10  NKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKITDSDRRGVP 69
           N+K K+V ++G T  GKS+LSVDLAT FPSEIINSDKIQ Y+GL+I TN+IT  DRRGVP
Sbjct: 62  NRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVP 121

Query: 70  HHLLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALV----ENPIT 129
           HHLLGVI     +LTAGEF       + EI  R ++PI+ GGSN+++ AL+    +    
Sbjct: 122 HHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 181

Query: 130 DFRS---------RFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVP---GA 189
            F S         R++CCF+W+DVS  VLY Y+ +RVD+M+++G+ EE+   + P   G 
Sbjct: 182 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 241

Query: 190 DYSRGIRRAIGAPEMDSYFKAERNDEESYKDDLLK-----SAIQEIKDNTCKLAFRQFGK 249
           +   GIR+AIG PE D YFK    +++  K D L+      A+ +IK NT  LA RQ  K
Sbjct: 242 ETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKK 301

Query: 250 IHRLRDELGWGLHRIDATAVFE------KSGEDAADEWMNAVLKPSLAIV 273
           I  L+D  GW + R+DATA F+       S +   + W   VL+PS+ IV
Sbjct: 302 IEMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIV 350

BLAST of Lag0001676 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 229.6 bits (584), Expect = 3.8e-60
Identity = 128/288 (44.44%), Postives = 177/288 (61.46%), Query Frame = 0

Query: 12  KHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKITDSDRRGVPHH 71
           K K+V +MG T +GKS LS+DLAT F  EI+NSDKIQFY GL + TN+++  +R GVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 72  LLGVIEDPDADLTAGEFCRLVEGSIAEIIGRGRLPIVVGGSNNYIEALVEN-------PI 131
           LLG +   D++LT  EF  L   SI+EI  RG LPI+ GGSN++I AL+ +       P 
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPF 161

Query: 132 TDFRS-----RFDCCFVWIDVSIPVLYRYIAKRVDQMVEAGLVEEVREMFVP----GADY 191
           +   S     R++CCF+W+DVS+ VL+ Y++KRVDQM+E+G+ EE+   + P     A  
Sbjct: 162 SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 221

Query: 192 SRGIRRAIGAPEMDSYFK---AERNDEESYKDDLLK----SAIQEIKDNTCKLAFRQFGK 251
           + GI + IG PE D YF     ER  + S  D   K     A+QEIK+NT +LA +Q  +
Sbjct: 222 AHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIER 281

Query: 252 IHRLRDELGWGLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFL 277
           I +L+   GW + R+DAT  F   G  + + W N VL  S+ +V  FL
Sbjct: 282 IMKLKSS-GWDIQRLDATPSF---GRSSREIWDNTVLDESIKVVKRFL 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888774.17.4e-15984.43adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida][more]
KAA0036515.13.1e-14981.79adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK13465.1 adenyl... [more]
XP_004148309.16.9e-14981.49adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus][more]
XP_008447303.11.2e-14881.49PREDICTED: adenylate isopentenyltransferase 5, chloroplastic-like [Cucumis melo][more]
XP_022972357.11.9e-13881.31adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q94ID23.5e-7954.28Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q93WC93.7e-7653.73Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID19.0e-7550.18Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q5GHF71.2e-6344.41Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID31.0e-6245.86Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A5D3CPK41.5e-14981.79Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0K7L13.4e-14981.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G253720 PE=4 SV=1[more]
A0A1S3BH375.7e-14981.49adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 G... [more]
A0A6J1I4L39.2e-13981.31adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A6J1GLR35.9e-13880.98adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
AT5G19040.12.5e-8054.28isopentenyltransferase 5 [more]
AT3G63110.12.6e-7753.73isopentenyltransferase 3 [more]
AT3G23630.16.4e-7650.18isopentenyltransferase 7 [more]
AT1G68460.17.3e-6445.86isopentenyltransferase 1 [more]
AT3G19160.13.8e-6044.44ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 142..240
e-value: 3.6E-106
score: 356.3
NoneNo IPR availablePFAMPF01715IPPTcoord: 130..226
e-value: 8.8E-13
score: 48.5
coord: 48..125
e-value: 1.2E-20
score: 74.2
NoneNo IPR availablePANTHERPTHR11088:SF67BINDING PROTEIN, PUTATIVE-RELATEDcoord: 7..279
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 12..276
e-value: 3.6E-106
score: 356.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 10..171
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 7..279

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0001676.1Lag0001676.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0052381 tRNA dimethylallyltransferase activity